CN116297416A - Photo-excitation chemiluminescence detection device and detection method - Google Patents
Photo-excitation chemiluminescence detection device and detection method Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 100
- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 20
- 230000001443 photoexcitation Effects 0.000 title claims description 14
- 239000013307 optical fiber Substances 0.000 claims abstract description 255
- 230000007246 mechanism Effects 0.000 claims abstract description 173
- 230000005284 excitation Effects 0.000 claims abstract description 63
- 230000003287 optical effect Effects 0.000 claims abstract description 52
- 238000009434 installation Methods 0.000 claims abstract description 43
- 238000007789 sealing Methods 0.000 claims description 59
- 239000000126 substance Substances 0.000 claims description 20
- 239000000835 fiber Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 18
- 230000004907 flux Effects 0.000 abstract description 7
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- 238000005516 engineering process Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 2
- 238000000504 luminescence detection Methods 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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- 230000002285 radioactive effect Effects 0.000 description 1
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- 239000010703 silicon Substances 0.000 description 1
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Abstract
The application relates to a light excitation chemiluminescence detection device and a detection method. The detection device comprises a carrying mechanism, a light excitation mechanism, a detection mechanism, an optical fiber installation module and a rotating mechanism, wherein the carrying mechanism is provided with a plurality of carrying positions for carrying the reaction container; the light excitation mechanism comprises an exciter and an emergent optical fiber; the detection mechanism comprises a detector and an incident optical fiber; the optical fiber installation module is provided with a plurality of optical fiber interfaces which are respectively connected with the emergent optical fiber and the incident optical fiber; the rotating mechanism enables the carrying mechanism and the optical fiber installation module to rotate relatively, so that reaction containers carried by carrying levels at different positions on the carrying mechanism are respectively opposite to optical fiber interfaces at different positions on the optical fiber installation module. According to the scheme, the structure of the light laser mechanism and the structure of the detection mechanism can be designed flexibly, laser irradiation and optical signal detection on different reaction containers can be executed synchronously, and the detection efficiency and the detection flux are improved.
Description
Technical Field
The application relates to the technical field of chemiluminescence immunoassay, in particular to a light-activated chemiluminescence detection device and a detection method.
Background
Chemiluminescent immunoassay is a non-radioactive immunoassay technology which has been developed rapidly in recent years, and a light-activated chemiluminescent analyzer mainly comprises a light-activated mechanism and a detection mechanism. The laser system generally adopts a 650-700 nm laser, and adopts proper optical power to carry out laser irradiation on the reaction liquid to be detected; the detection mechanism mainly adopts a high-sensitivity photomultiplier as a detector, the reaction liquid to be detected emits 500-620 nm optical signals after being irradiated by 650-700 nm laser, the optical signals are collected by the photomultiplier and then output weak current signals, and the current signals are amplified and converted into pulse signals to be output and read and output by computer upper computer software.
In the related art, the optical laser mechanism and the detection mechanism are generally separated by the shutter assembly, so that the structures of the optical laser mechanism and the detection mechanism cannot be flexibly designed due to the limitation of the optical path structure, and the structure is complex and large in size. And the detection efficiency and the detection flux are low because the substances to be detected in the reaction vessel can be detected successively only under the limitation of the structures of the light excitation mechanism and the detection mechanism.
Disclosure of Invention
In order to solve or partially solve the problems existing in the related art, the application provides a light excitation chemiluminescence detection device and a detection method, which can flexibly design structures of a light excitation mechanism and a detection mechanism, synchronously execute laser irradiation and optical signal detection on different reaction vessels, and improve detection efficiency and detection flux.
A first aspect of the present application provides a photoexcitation chemiluminescent detection device, comprising:
the carrying mechanism is provided with a plurality of carrying positions for carrying the reaction containers;
the light excitation mechanism comprises an exciter and an emergent optical fiber, and the emergent optical fiber is used for emergent excitation light of the exciter;
the detection mechanism comprises a detector and an incident optical fiber, wherein the incident optical fiber is used for incident light signals to the detector;
the optical fiber installation module is provided with a plurality of optical fiber interfaces, and the optical fiber interfaces are respectively connected with the emergent optical fiber and the incident optical fiber;
and the rotating mechanism enables the carrying mechanism and the optical fiber installation module to relatively rotate, so that reaction containers carried by the carrying levels at different positions on the carrying mechanism are respectively opposite to the optical fiber interfaces at different positions on the optical fiber installation module.
As an alternative embodiment, the rotation mechanism is configured to rotate the carrying mechanism and/or the fiber optic mounting module.
As an alternative embodiment, the optical fiber installation module comprises a turntable and a light-sealing structure, the optical fiber interface is arranged on the turntable, and the light-sealing structure is slidably arranged in the optical fiber interface; the method comprises the steps of,
the light-dense structure is configured to: the optical fiber interface can move upwards relative to the rotary disc when the optical fiber interface contacts the reaction container, and can move downwards relative to the rotary disc to seal the reaction cup opening of the reaction container when the optical fiber interface is opposite to the reaction container.
As an alternative embodiment:
the light-sealing structure comprises a light-sealing piece, a sliding sleeve is sleeved on the light-sealing piece, and the light-sealing piece is arranged in the optical fiber interface in a sliding manner through the sliding sleeve; and/or the number of the groups of groups,
the light-sealing structure further comprises an elastic piece, wherein the elastic piece is arranged between the light-sealing piece and the optical fiber interface, and when the optical fiber interface is opposite to the reaction container, the elastic piece resets and drives the light-sealing piece to move downwards to an initial position relative to the rotary disc.
As an alternative embodiment, the outer circumference of the turntable is provided with a light shielding ring in a protruding way, and the light shielding ring is used for realizing the light tightness of the reaction cup opening of the reaction container when the optical fiber interface is switched between the reaction containers at different positions.
As an alternative embodiment:
the plurality of carrying positions of the carrying mechanism comprise reaction container positions, excitation cup positions, detection cup positions and discarding cup positions, and the reaction container positions, the excitation cup positions, the detection cup positions and the discarding cup positions are distributed at intervals along the circumferential direction of the carrying mechanism; and the initial positions of the excitation cup position and the detection cup position respectively correspond to a first optical fiber interface connected with the emergent optical fiber and a second optical fiber interface communicated with the incident optical fiber.
As an optional embodiment, the inner wall of the reaction vessel is provided with a diffuse reflection structure, and the diffuse reflection structure is used for diffusely reflecting the optical signal generated by the substance to be detected in the reaction vessel into the incident optical fiber.
A second aspect of the present application provides a light-activated chemiluminescence detection method, including:
s1, providing the light-activated chemiluminescence detection device, and driving the optical fiber installation module to move to the position above a reaction container in the loading level;
s2, enabling the carrying mechanism and the optical fiber installation module to relatively rotate so as to enable a first optical fiber interface connected with the emergent optical fiber to be opposite to the reaction container;
s3, the exciter irradiates laser into the opposite reaction container through the emergent optical fiber;
s4, enabling the carrying mechanism and the optical fiber installation module to relatively rotate so that the excited reaction container is opposite to a second optical fiber interface connected with the incident optical fiber;
s5, the incidence optical fiber is used for incidence of the optical signal generated by the excited reaction vessel to the detector, and the optical signal of the excited reaction vessel is detected;
s6, repeatedly executing the steps S2 to S5.
As an alternative embodiment:
in the step S4, when the carrying mechanism and the optical fiber installation module are rotated relatively, the first optical fiber interface is also opposite to another reaction container;
step S5 further includes: the exciter irradiates laser into the other opposite reaction container through the emergent optical fiber.
As an alternative embodiment, before driving the fiber installation module to move above the reaction container in the loading level in step S1, the method includes:
loading a plurality of incubated reaction vessels to the load level; or loading a plurality of empty reaction containers to the loading level, and then injecting the reaction liquid into the plurality of empty reaction containers and heating.
The technical scheme that this application provided can include following beneficial effect:
according to the embodiment of the application, the object carrying mechanism and the optical fiber installation module are enabled to rotate relatively through the rotating mechanism, so that the incident optical fiber and the emergent optical fiber can be opposite to the reaction containers at different positions on the object carrying mechanism through the optical fiber interfaces at different positions, laser irradiation and optical signal detection on different reaction containers can be synchronously executed, and the detection efficiency is improved. The optical excitation mechanism and the detection mechanism are mutually independent and do not interfere with each other, and the detection mechanism transmits optical signals generated by the substances to be detected in the reaction container after excitation into the detector through the incident optical fiber, so that a mode of collecting optical signals by adopting a complex lens (an aspheric lens) or a spherical lens with multiple incident planes as planes in the related technology is replaced; furthermore, the excitation light generated by the exciter is transmitted into the reaction container by the light excitation mechanism through the emergent optical fiber, the light signal generated by the substance to be detected in the reaction container after excitation is transmitted into the detector by the detection mechanism through the incident optical fiber, the light excitation mechanism and the detection mechanism work independently, and the light excitation mechanism and the detection mechanism do not need to be separated through the shutter assembly, so that the structures of the light excitation mechanism and the detection mechanism are not limited by the light path structure, the structures of the light excitation mechanism and the detection mechanism can be flexibly designed, and the cost of the light excitation chemiluminescence detection device is reduced. And the substances to be detected in a small gap can be guided to a position with larger space in an optical fiber conduction mode, so that the simplicity and the flexibility of the structural design of the light laser mechanism and the detection mechanism are improved.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a schematic diagram of a photo-activated chemiluminescent detection device according to an embodiment of the present application;
FIG. 2 is a full cross-sectional view of a fiber optic installation module shown in an embodiment of the present application;
fig. 3 is a schematic structural diagram of an object carrying mechanism according to an embodiment of the present application.
In the figure:
1. a loading mechanism; 10. carrying a material level; 10a, reaction vessel position; 10b, exciting the cup position; 10c, detecting the cup position; 10d, discarding the cup position;
2. a reaction vessel;
3. a light excitation mechanism; 30. an exciter; 31. an exit optical fiber;
4. a detection mechanism; 40. a detector; 41. an incident optical fiber;
5. an optical fiber installation module; 50. an optical fiber interface; 50a, a first fiber optic interface; 50b, a second fiber optic interface; 51. a turntable; 52. a light-dense structure; 520. a light-sealing member; 521. a sliding sleeve; 522. an elastic member; 53. a shading ring.
Detailed Description
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the related art, the optical laser mechanism and the detection mechanism are generally separated by the shutter assembly, so that the structures of the optical laser mechanism and the detection mechanism cannot be flexibly designed due to the limitation of the optical path structure, and the structure is complex and large in size. And the detection efficiency and the detection flux are low because the substances to be detected in the reaction vessel can be detected successively only under the limitation of the structures of the light excitation mechanism and the detection mechanism.
In view of the above problems, embodiments of the present application provide a light excitation chemiluminescence detection device, which can flexibly design structures of a light excitation mechanism and a detection mechanism, and can synchronously perform laser irradiation and optical signal detection on different reaction vessels, thereby improving detection efficiency and detection flux.
The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.
Fig. 1 is a schematic structural diagram of a photo-excitation chemiluminescent detection device according to an embodiment of the present application.
Referring to fig. 1, an embodiment of the present application provides a light excitation chemiluminescence detection device, including a carrying mechanism 1, a light excitation mechanism 3, a detection mechanism 4, an optical fiber installation module 5, and a rotation mechanism (not shown in the figure), where the carrying mechanism 1 has a plurality of carrying levels 10 for carrying a reaction vessel 2; the light excitation mechanism 3 comprises an exciter 30 and an emergent optical fiber 31, wherein the emergent optical fiber 31 is used for emergent excitation light of the exciter 30; the detection mechanism 4 includes a detector 40 and an incident optical fiber 41, the incident optical fiber 41 being for incident optical signals to the detector 40; the optical fiber installation module 5 is provided with a plurality of optical fiber interfaces 50, and the optical fiber interfaces 50 are respectively connected with the emergent optical fiber 31 and the incident optical fiber 41, wherein the optical fiber interface 50 connected with the emergent optical fiber 31 is a first optical fiber interface 50a, and the optical fiber interface 50 connected with the incident optical fiber 41 is a second optical fiber interface 50b; the rotation mechanism enables the carrying mechanism 1 and the optical fiber installation module 5 to rotate relatively (wherein the positions of the exciter 30 and the detector 40 are kept unchanged and cannot rotate along with the optical fiber installation module 5), so that the reaction containers 2 carried by the carrying positions 10 at different positions on the carrying mechanism 1 are respectively opposite to the optical fiber interfaces 50 at different positions on the optical fiber installation module 5.
The working principle of the photo-excitation chemiluminescence detection device in the embodiment of the application is as follows:
if the carrying mechanism 1 is in the initial no-load running state, loading the reaction vessel 2 to the carrying level 10, and relatively rotating the carrying mechanism 1 and the optical fiber installation module 5 by the rotating mechanism, so that the outgoing optical fiber 31 and the incoming optical fiber 41 respectively correspond to the reaction vessel 2 in different positions through the optical fiber interfaces 50 (i.e. the first optical fiber interface 50a and the second optical fiber interface 50 b) in different positions; the exciter 30 then irradiates the opposite reaction vessel 2 with laser light via the exit optical fiber 31. And then the carrying mechanism 1 and the optical fiber mounting module 5 are rotated relatively again, the excited reaction vessel is opposite to the optical fiber interface 50 (namely the second optical fiber interface 50 b) connected with the incident optical fiber 41, the incident optical fiber 41 is used for making an optical signal incident on the detector 40, and the detector 40 is used for detecting the optical signal of the excited reaction vessel, so that the first batch of detection tasks are completed. In the optical signal detection in the first batch of detection task, the optical fiber interface 50 (i.e., the first optical fiber interface 50 a) connected to the incident optical fiber 41 is simultaneously opposite to the other reaction vessel, the exciter 30 irradiates laser into the opposite other reaction vessel through the outgoing optical fiber 31, and then rotates to detect the optical signal of the excited other reaction vessel, so as to complete the second detection task. Therefore, the rotating mechanism enables the carrying mechanism 1 and the optical fiber installation module 5 to rotate relatively, so that the incident optical fiber 41 and the emergent optical fiber 31 can be opposite to the reaction vessels 2 at different positions on the carrying mechanism 1 through the optical fiber interfaces 50 at different positions, laser irradiation and optical signal detection on different reaction vessels 2 can be synchronously performed, and detection efficiency and detection flux are improved.
The excitation light that the excitation mechanism 3 produced the exciter 30 through the emergent optical fiber 31 is conducted to the reaction vessel 2, the detection mechanism 4 is conducted to the detector 40 through the incident optical fiber 41 after excitation of the substance to be detected in the reaction vessel 2, the light excitation mechanism 3 and the detection mechanism 4 work independently of each other, the structure of the light excitation mechanism 3 and the structure of the detection mechanism 4 are not limited by the light path structure without separating through a shutter assembly, and therefore the structures of the light excitation mechanism 3 and the detection mechanism 4 can be flexibly designed, and the cost of the light excitation chemical luminescence detection device is reduced. And the substances to be detected in a small gap can be led to a position with larger space in a mode of optical fiber conduction, so that the simplicity and the flexibility of the structural design of the light excitation mechanism 3 and the detection mechanism 4 are improved.
Moreover, since the optical fibers are soft optical fibers, a certain degree of folding and winding can be performed, when the object carrying mechanism 1 and the optical fiber mounting module 5 relatively rotate, the positions of the first optical fiber interface 50a and the second optical fiber interface 50b are changed, in order to connect the incident optical fiber 41 and the emergent optical fiber 31 with the first optical fiber interface 50a and the second optical fiber interface 50b again respectively, the optical fibers can be rotated, wound, folded, moved and the like to realize the connection of the incident optical fiber 41 and the emergent optical fiber 31 with the first optical fiber interface 50a and the second optical fiber interface 50b respectively, so that when the positions of the first optical fiber interface 50a and the second optical fiber interface 50b are changed, the positions of the exciter 30 and the detector 40 away from one end of the exciter 30 and the detector 40 can be changed, and the incident optical fiber 41 and the emergent optical fiber 31 can be always kept connected with the first optical fiber interface 50a and the second optical fiber interface 50b, thereby corresponding operations can be performed on the reaction containers 2 at different positions. In addition, the optical signals are conducted through the outgoing optical fiber 31 and the incoming optical fiber 41, so that only one ends of the outgoing optical fiber 31 and the incoming optical fiber 41, which are close to the exciter 30 and the detector 40, are required to be aligned with the exciter 30 and the detector 40, and the exciter 30 and the detector 40 are not required to be positioned by other mechanical structures and debugged, so that the detection efficiency is greatly improved. Moreover, the incident optical fiber 41 of the embodiment of the present application has strong optical signal transmission capability and small optical power loss, and accordingly improves the detection efficiency.
As an alternative embodiment, a rotation mechanism is used to drive the rotation of the loading mechanism 1 and/or the fiber mounting module 5.
The rotary mechanism of the embodiment of the application has three driving modes:
1. the rotation mechanism only drives the loading mechanism 1 to rotate, and the optical fiber mounting module 5 does not rotate. This mode is more suitable for a small-structured carrier mechanism 1, and this drive mode is preferred in the embodiment of the present application.
2. The rotation mechanism only drives the optical fiber mounting module 5 to rotate, and the loading mechanism 1 does not rotate. This mode can prevent the substances to be measured in the reaction vessel 2 from being scattered during the rotation of the carrying mechanism 1.
3. The rotation mechanism simultaneously drives the loading mechanism 1 and the optical fiber mounting module 5 to rotate. This mode can improve the detection efficiency, and the accuracy of the execution mode of the whole photo-excitation chemiluminescence detection device is required to be higher.
As a preferred embodiment, the rotation mechanism is used to drive the rotation of the loading mechanism 1 and/or the fiber mounting module 5 for periodic rotation.
In the above three modes, since the time required for each link is known and the relative positions between the reaction vessels 2 at different positions are also known during the execution of the detection task, and the rotation speed is adjustable, the rotation time of each link can be designed so that the carrying mechanism 1 and/or the optical fiber mounting module 5 can be periodically rotated, thereby improving the versatility of the photo-activated chemiluminescence detection device.
As an alternative embodiment, referring to fig. 2, the optical fiber mounting module 5 includes a turntable 51 and a light-sealing structure 52, the optical fiber interface 50 is disposed on the turntable 51, and the light-sealing structure 52 is slidably disposed in the optical fiber interface 50; and, the light-dense structure 52 is configured to: the optical fiber interface device can move upwards relative to the rotary disc 51 when the light-sealing structure 52 touches the reaction vessel 2, and can move downwards relative to the rotary disc 51 to seal the reaction cup opening of the reaction vessel 2 when the optical fiber interface 50 is opposite to the reaction vessel 2.
In the process of relatively rotating the carrying mechanism 1 and the optical fiber installation module 5, when the optical fiber interface 50 is about to be opposite to the reaction container 2, the light-sealing structure 52 will first touch the reaction container 2, and when the light-sealing structure 52 touches the reaction container 2, the light-sealing structure 52 can move upwards relative to the turntable 51, so that the carrying mechanism 1 and the optical fiber installation module 5 can continue to relatively rotate; then, the object carrying mechanism 1 and the optical fiber mounting module 5 continue to rotate relatively until the optical fiber interface 50 is opposite to the reaction vessel 2 (i.e. the optical fiber interface 50 is located right above the reaction cup opening of the reaction vessel 2), at this time, the light-sealing structure 52 can move downwards relative to the turntable 51 to seal the reaction cup opening of the reaction vessel 2, so as to realize the light-sealing property of the optical fiber interface 50 and the reaction cup opening of the opposite reaction vessel 2 when the laser irradiates and detects the optical signal.
As a preferred embodiment, referring to FIG. 2, the light-sealing structure 52 comprises a light-sealing member 520, wherein the light-sealing member 520 is sleeved with a sliding sleeve 521, and the light-sealing member 520 is slidably arranged in the optical fiber interface 50 through the sliding sleeve 521.
The light-sealing member 520 in the embodiment of the present application is slidably disposed in the optical fiber interface 50 through the sliding sleeve 521, so that the light-sealing member 520 can move up and down relative to the turntable 51, thereby realizing the light sealing of the light-sealing member 520 to the reaction cup opening of the reaction vessel 2.
In addition, the light-sealing member 520 of the embodiment of the present application is impermeable to natural light, so as to prevent the natural light of the external environment from affecting the photo-activated chemiluminescent reaction in the reaction vessel 2. However, the light-sealing member 520 has a hollow structure, and the optical fiber may be inserted therein, that is, the inside of the light-sealing member 520 may be transparent to the excitation light and the optical signal, so that the excitation light is not affected to enter the reaction vessel 2 through the light-sealing member 520, and the optical signal is not affected to exit into the detector 40 through the light-sealing member 520. As a preferred embodiment, referring to fig. 2, the light-sealing structure 52 further includes an elastic member 522, where the elastic member 522 is disposed between the light-sealing member 520 and the optical fiber interface 50, and when the optical fiber interface 50 is opposite to the reaction vessel 2, the elastic member 522 resets and drives the light-sealing member 520 to move downward to an initial position relative to the turntable 51.
In the process of relatively rotating the carrying mechanism 1 and the optical fiber installation module 5, when the optical fiber interface 50 is about to be opposite to the reaction vessel 2, the light-sealing member 520 will first touch the reaction vessel 2, and when the light-sealing member 520 touches the reaction vessel 2, the light-sealing member 520 can move upwards relative to the turntable 51 through the sliding sleeve 521, and compresses the elastic member 522, so that the elastic member 522 is elastically deformed; then, the object carrying mechanism 1 and the optical fiber mounting module 5 continue to rotate relatively until the optical fiber interface 50 is opposite to the reaction vessel 2, and at this time, the elastic piece 522 resets and drives the light sealing piece 520 to move downwards to an initial position relative to the turntable 51, so that the light sealing piece 520 seals the reaction cup opening of the reaction vessel 2, thereby realizing the light sealing property of the optical fiber interface 50 and the reaction cup opening of the opposite reaction vessel 2 when the laser irradiates and detects the optical signal. As a preferred embodiment, referring to fig. 2, the outer circumference of the turntable 51 is formed with a light shielding ring 53 protruding downward, and the light shielding ring 53 is used to realize the light tightness of the reaction cup opening of the reaction vessel 2 when the optical fiber interface 50 is switched between the reaction vessels 2 at different positions.
Since the reaction cup opening of the reaction vessel 2 loses the light-tight protection of the light-tight member 520 when the optical fiber interface 50 is switched between the reaction vessels 2 at different positions, there is a risk of being affected by external natural light. Therefore, in the embodiment of the present application, a circle of light shielding ring 53 is protruding downwards on the outer circumference of the turntable 51, so as to realize the light tightness of the reaction cup opening of the reaction vessel 2 in the switching process. And the height of the light shielding ring 53 is not higher than the height of the light-sealing member 520 to maximally realize the sealing of the reaction cup opening of the reaction vessel 2.
As an alternative embodiment, referring to fig. 3, the plurality of carrier positions 10 of the carrier mechanism 1 include a reaction container position 10a, an excitation cup position 10b, a detection cup position 10c, and a discard cup position 10d, and the reaction container position 10a, the excitation cup position 10b, the detection cup position 10c, and the discard cup position 10d are distributed at intervals along the circumferential direction of the carrier mechanism 1; and the initial positions of the excitation cup 10b and the detection cup 10c correspond to the first optical fiber interface 50a connected to the outgoing optical fiber 31 and the second optical fiber interface 50b connected to the incoming optical fiber 41, respectively.
For the case of four load levels 10, taking the rotation of the load mechanism 1 as an example, the periodic operation of the load mechanism 1 in the embodiment of the present application will be described as follows:
the carrying mechanism 1 (reading turntable) rotates anticlockwise, and rotates one cell (one cell is one cup position) in each cycle.
The first cycle: the reaction vessel 2 is loaded into the reaction vessel station 10a.
The second cycle: the reaction vessel 2 at the reaction vessel position 10a is turned to the excitation cup position 10b, and in the latter half of the period, the exciter 30 emits excitation light (for example, 650nm-700nm red light), the emergent optical fiber 31 is conducted to the first optical fiber interface 50a through the emergent optical fiber 31, and then the excitation light is conducted to the reaction vessel 2 at the reaction vessel position 10a through the first optical fiber interface 50a, so as to excite the substance to be measured in the reaction vessel 2.
Third cycle: the carrying mechanism 1 is driven to rotate, the reaction container 2 positioned at the excitation cup position 10b is rotated to the detection cup position 10c, in the first half period, substances to be detected which are excited in the second period are excited, 615 nm-wavelength optical signals generated by excitation are led into the incident optical fiber 41 through the second optical fiber interface 50b, and then are conducted onto the detection surface of the detector 40 through the incident optical fiber 41; the light with the unnecessary interference wavelength and the impurity wavelength is filtered by the filter after being processed by different filters, and the wavelength actually reflected by the substance to be detected can be collected on the detector 40 in a pure way; the final detector 40 converts the optical signal into an electrical signal and measures the specific concentration of the substance to be measured.
Fourth cycle: the reaction vessel 2 at the detection cup position 10c is transferred to the discard cup position 10d, and the reaction vessel 2 is removed.
As an alternative embodiment, the inner wall of the reaction vessel 2 is provided with a diffuse reflection structure for diffusely reflecting the light signal generated by the substance to be measured in the reaction vessel 2 into the incident optical fiber 41.
The embodiment of the present application irradiates the luminescence signal into the incident optical fiber 41 at a plurality of angles by diffuse emission of the inner wall of the reaction vessel 2. In addition, the diffuse reflection structure may be a protrusion or a depression provided on the inner wall of the reaction vessel 2, or may be a diffuse reflection coating coated on the inner wall of the reaction vessel 2.
As an alternative embodiment, the detector 40 is a single photon counter, photomultiplier tube, silicon photocell, or photometry integrating sphere; the wavelength of the luminescence signal that can be detected by the detector 40 is 500 to 620nm. Corresponding to the embodiment of the application function implementation method, the application also provides a light excitation chemiluminescence detection method and a corresponding embodiment. A method of photoexcitation chemiluminescence detection, comprising:
s1, providing the light-activated chemiluminescence detection device, and driving the optical fiber installation module 5 to move to the position above the reaction vessel 2 in the loading level 10.
Before driving the optical fiber installation module 5 to move above the reaction vessel 2 in the loading level in step S1 of this embodiment of the present application, the method includes:
loading a plurality of incubated reaction vessels to a load level 10; alternatively, a plurality of empty reaction containers are loaded on the carrier level 10, and then the reaction liquid (for example, a sample and a reagent) is injected into the plurality of empty reaction containers and heated (for example, heated by a heating module provided in the carrier mechanism 1).
S2, the carrying mechanism 1 and the optical fiber mounting module 5 are rotated relatively so that the first optical fiber interface 50a connected with the emergent optical fiber 31 is opposite to the reaction container 2.
If the loading mechanism 1 is in the initial no-load operation state, the reaction vessel 2 is loaded to the loading position 10, and the rotating mechanism makes the loading mechanism 1 and the optical fiber installation module 5 rotate relatively, so that the outgoing optical fiber 31 and the incoming optical fiber 41 respectively correspond to the reaction vessel 2 in different positions through the optical fiber interfaces 50 (i.e. the first optical fiber interface 50a and the second optical fiber interface 50 b) in different positions.
S3, the exciter 30 irradiates the inside of the opposing reaction vessel 2 with laser light through the emission optical fiber 31.
The exciter 30 irradiates the opposite reaction vessel 2 with laser light through the outgoing optical fiber 31, but since the reaction vessel 2 corresponding to the incoming optical fiber 41 has not been irradiated with laser light yet in the initial operation, the detector 40 and the incoming optical fiber 41 do not perform the detection task in the initial operation state.
S4, the carrying mechanism 1 and the optical fiber installation module 5 are rotated relatively, so that the excited reaction vessel is opposite to the second optical fiber interface 50b connected with the incident optical fiber 41.
The loading mechanism 1 is then rotated relative to the fiber mounting module 5 again so that the excited reaction vessel is opposite the fiber interface 50 (i.e., the second fiber interface 50 b) to which the incident fiber 41 is connected.
S5, the incidence optical fiber 41 is used for incidence of the optical signal generated by the excited reaction vessel to the detector 40, and the detector 40 is used for detecting the optical signal of the excited reaction vessel.
The incident optical fiber 41 irradiates the optical signal generated by the excited reaction vessel to the detector 40, and the detector 40 detects the optical signal of the excited reaction vessel, thereby completing the first batch of detection tasks.
As an alternative embodiment, when the carrier mechanism 1 and the fiber mounting module 5 are driven to rotate relatively in step S4, the first fiber interface 50a is also made to face another reaction container. Step S5 further includes: the exciter 30 irradiates laser light into the other reaction vessel via the emission optical fiber 31.
In the optical signal detection in the first batch of detection task, the optical fiber interface 50 (i.e., the first optical fiber interface 50 a) connected to the incident optical fiber 41 is simultaneously opposite to the other reaction vessel, the exciter 30 irradiates laser into the opposite other reaction vessel through the outgoing optical fiber 31, and then rotates to detect the optical signal of the excited other reaction vessel, so as to complete the second detection task.
S6, repeatedly executing the steps S2 to S5.
According to the embodiment of the application, the object carrying mechanism 1 and the optical fiber installation module 5 are driven to rotate relatively through the rotating mechanism, so that the incident optical fiber 41 and the emergent optical fiber 31 are opposite to the reaction containers 2 at different positions on the object carrying mechanism 1 through the first optical fiber interface 50a and the second optical fiber interface 50b respectively, laser irradiation and optical signal detection on different reaction containers 2 can be synchronously executed, and the detection efficiency and the detection flux are improved.
According to the optical excitation mechanism 3, excitation light generated by the exciter 30 is conducted into the reaction container 2 through the emergent optical fiber 31, the detection mechanism 4 conducts light signals generated by the substances to be detected in the reaction container 2 after excitation into the detector 40 through the incident optical fiber 41, the optical excitation mechanism 3 and the detection mechanism 4 work independently, the optical excitation mechanism 3 and the detection mechanism 4 do not need to be separated through a shutter assembly, the structures of the optical excitation mechanism 3 and the detection mechanism 4 are not limited by the light path structure, and therefore the structures of the optical excitation mechanism 3 and the detection mechanism 4 can be flexibly designed, and the cost of the optical excitation chemical luminescence detection device is reduced. And the substances to be detected in a small gap can be led to a position with larger space in a mode of optical fiber conduction, so that the simplicity and the flexibility of the structural design of the light excitation mechanism 3 and the detection mechanism 4 are improved.
As an alternative embodiment, the driving the carrier mechanism 1 to rotate relative to the optical fiber mounting module 5 in step S2 and step S4 includes: the loading mechanism 1 and/or the fiber mounting module 5 are driven to rotate.
The rotary mechanism of the embodiment of the application has three driving modes:
1. the rotation mechanism only drives the loading mechanism 1 to rotate, and the optical fiber mounting module 5 does not rotate. This mode is applicable to a small-structure carrier mechanism 1.
2. The rotation mechanism only drives the optical fiber mounting module 5 to rotate, and the loading mechanism 1 does not rotate. This mode can prevent the substances to be measured in the reaction vessel 2 from being scattered during the rotation of the carrying mechanism 1.
3. The rotation mechanism simultaneously drives the loading mechanism 1 and the optical fiber mounting module 5 to rotate. This mode can improve the detection efficiency, and the accuracy of the execution mode of the whole photo-excitation chemiluminescence detection device is required to be higher.
As an alternative embodiment, when the carrier mechanism 1 is driven to rotate relative to the optical fiber mounting module 5 in step S2 and step S4, the method further includes:
when the light-sealing structure 52 of the optical fiber installation module 5 touches the reaction vessel 2, the light-sealing structure 52 moves upwards relative to the turntable 51 of the optical fiber installation module 5, and when the optical fiber interface 50 is opposite to the reaction vessel 2, the light-sealing structure 52 moves downwards relative to the turntable 51 to seal the reaction cup opening of the reaction vessel 2.
In the process of relatively rotating the carrying mechanism 1 and the optical fiber installation module 5, when the optical fiber interface 50 is about to be opposite to the reaction container 2, the light-sealing structure 52 will first touch the reaction container 2, and when the light-sealing structure 52 touches the reaction container 2, the light-sealing structure 52 can move upwards relative to the turntable 51, so that the carrying mechanism 1 and the optical fiber installation module 5 can continue to relatively rotate; then, the object carrying mechanism 1 and the optical fiber mounting module 5 continue to rotate relatively until the optical fiber interface 50 is opposite to the reaction vessel 2 (i.e. the optical fiber interface 50 is located right above the reaction cup opening of the reaction vessel 2), at this time, the light-sealing structure 52 can move downwards relative to the turntable 51 to seal the reaction cup opening of the reaction vessel 2, so as to realize the light-sealing property of the first optical fiber interface 50a and the reaction cup opening of the opposite reaction vessel 2 when the laser irradiates, and the light-sealing property of the second optical fiber interface 50b and the reaction cup opening of the opposite reaction vessel 2 when the optical signal is detected.
The aspects of the present application have been described in detail hereinabove with reference to the accompanying drawings. In the foregoing embodiments, the descriptions of the embodiments are focused on, and for those portions of one embodiment that are not described in detail, reference may be made to the related descriptions of other embodiments. Those skilled in the art will also appreciate that the acts and modules referred to in the specification are not necessarily required in the present application. In addition, it can be understood that the steps in the method of the embodiment of the present application may be sequentially adjusted, combined and pruned according to actual needs, and the modules in the apparatus of the embodiment of the present application may be combined, divided and pruned according to actual needs.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A photo-activated chemiluminescent detection device comprising:
a loading mechanism (1), wherein the loading mechanism (1) is provided with a plurality of loading positions (10) for loading the reaction container (2);
a light excitation mechanism (3), wherein the light excitation mechanism (3) comprises an exciter (30) and an emergent optical fiber (31), and the emergent optical fiber (31) is used for emergent excitation light of the exciter (30);
-a detection mechanism (4), the detection mechanism (4) comprising a detector (40) and an incident optical fiber (41), the incident optical fiber (41) being for incident optical signals to the detector (40);
the optical fiber installation module (5), a plurality of optical fiber interfaces (50) are arranged on the optical fiber installation module (5), and the optical fiber interfaces (50) are respectively connected with the emergent optical fiber (31) and the incident optical fiber (41);
the rotating mechanism enables the carrying mechanism (1) and the optical fiber installation module (5) to rotate relatively, so that reaction containers (2) carried by the carrying positions (10) at different positions on the carrying mechanism (1) are respectively opposite to the optical fiber interfaces (50) at different positions on the optical fiber installation module (5).
2. The light-activated chemiluminescent detection arrangement according to claim 1, characterized in that the rotation mechanism is adapted to drive the rotation of the carrier mechanism (1) and/or the fiber attachment module (5).
3. The photoexcitation chemiluminescent detection device according to claim 1, wherein the fiber attachment module (5) includes a turntable (51) and a light-tight structure (52), the fiber interface (50) is provided on the turntable (51), and the light-tight structure (52) is slidably provided within the fiber interface (50); the method comprises the steps of,
the light-dense structure (52) is configured to: the optical fiber interface (50) can move upwards relative to the rotary disc (51) when the light-sealing structure (52) contacts the reaction container (2), and can move downwards relative to the rotary disc (51) to seal the reaction cup opening of the reaction container (2) when the optical fiber interface (50) is opposite to the reaction container (2).
4. The photoexcitation chemiluminescent detection system of claim 3, wherein:
the light-sealing structure (52) comprises a light-sealing piece (520), a sliding sleeve (521) is sleeved on the light-sealing piece (520), and the light-sealing piece (520) is slidably arranged in the optical fiber interface (50) through the sliding sleeve (521); and/or the number of the groups of groups,
the light-sealing structure (52) further comprises an elastic piece (522), wherein the elastic piece (522) is arranged between the light-sealing piece (520) and the optical fiber interface (50), and when the optical fiber interface (50) is opposite to the reaction container (2), the elastic piece (522) resets and drives the light-sealing piece (520) to move downwards to an initial position relative to the rotary disc (51).
5. A photoexcitation chemiluminescent detection unit according to claim 3, wherein a light shielding ring (53) is formed to protrude downward from an outer circumference of the turntable (51), and the light shielding ring (53) is used for realizing the light tightness of a reaction cup opening of the reaction vessel (2) when the optical fiber interface (50) is switched between reaction vessels (2) at different positions.
6. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the plurality of carrying positions (10) of the carrying mechanism (1) comprise reaction container positions (10 a), excitation cup positions (10 b), detection cup positions (10 c) and discarding cup positions (10 d), and the reaction container positions (10 a), the excitation cup positions (10 b), the detection cup positions (10 c) and the discarding cup positions (10 d) are distributed at intervals along the circumferential direction of the carrying mechanism (1); and the initial positions of the excitation cup position (10 b) and the detection cup position (10 c) respectively correspond to a first optical fiber interface (50 a) connected with the emergent optical fiber (31) and a second optical fiber interface (50 b) communicated with the incident optical fiber (41).
7. The photoexcitation chemiluminescence detection device according to claim 1, wherein a diffuse reflection structure is provided on an inner wall of the reaction vessel (2), and the diffuse reflection structure is used for diffusely reflecting an optical signal generated by a substance to be detected in the reaction vessel (2) into the incident optical fiber (41).
8. A method of photoexcitation chemiluminescence detection, comprising:
s1, providing a light-activated chemiluminescence detection device according to any one of claims 1-7, and driving the optical fiber installation module (5) to move above a reaction container (2) in the carrying level (10);
s2, enabling the carrying mechanism (1) and the optical fiber installation module (5) to relatively rotate so as to enable a first optical fiber interface (50 a) connected with the emergent optical fiber (31) to be opposite to the reaction container (2);
s3, the exciter (30) irradiates laser into the opposite reaction container (2) through the emergent optical fiber (31);
s4, enabling the carrying mechanism (1) and the optical fiber installation module (5) to relatively rotate so as to enable the excited reaction container to be opposite to a second optical fiber interface (50 b) connected with the incident optical fiber (41);
s5, the incidence optical fiber (41) is used for incidence of the optical signal generated by the excited reaction vessel to the detector (40) and detecting the optical signal of the excited reaction vessel;
s6, repeatedly executing the steps S2 to S5.
9. The method for detecting photo-activated chemiluminescence according to claim 8, wherein:
in the step S4, when the carrying mechanism (1) and the optical fiber installation module (5) are rotated relatively, the first optical fiber interface (50 a) is also opposite to another reaction container;
step S5 further includes: the exciter (30) irradiates laser light into the other reaction vessel which is opposite to the other reaction vessel through the emergent optical fiber (31).
10. The method according to claim 8, wherein before driving the fiber mounting module (5) to move above the reaction vessel (2) in the loading level (10) in step S1, the method comprises:
loading a plurality of incubated reaction vessels to the load level (10); alternatively, a plurality of empty reaction vessels are loaded on the loading level (10), and then the reaction solution is injected into the plurality of empty reaction vessels and heated.
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