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CN116136495A - Method for Simultaneously Detecting Protein Content and Fat Content in Grain-Containing Milk - Google Patents

Method for Simultaneously Detecting Protein Content and Fat Content in Grain-Containing Milk Download PDF

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Publication number
CN116136495A
CN116136495A CN202111360944.6A CN202111360944A CN116136495A CN 116136495 A CN116136495 A CN 116136495A CN 202111360944 A CN202111360944 A CN 202111360944A CN 116136495 A CN116136495 A CN 116136495A
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milk
content
fat
protein content
fat content
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董勇
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Inner Mongolia Yili Industrial Group Co Ltd
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Inner Mongolia Yili Industrial Group Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water

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  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
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Abstract

The invention provides a method for simultaneously detecting the protein content and the fat content in milk containing particles. The method comprises the following steps: and (3) temperature adjustment: heating the milk containing the particles to 30-55deg.C to obtain temperature-regulating milk; and (3) filtering: filtering the temperature-regulated milk to obtain filtered milk; and (3) infrared spectrum analysis: and carrying out infrared spectrum analysis on the filtered milk to obtain the content of protein and fat. The method can shorten the detection time, improve the production efficiency, ensure that production personnel can accurately control the production process by higher detection accuracy and more detection index items, and achieve the effect of accurately dosing and controlling the production cost.

Description

Method for simultaneously detecting protein content and fat content in milk containing particles
Technical Field
The invention belongs to the technical field of component detection in milk products, and particularly relates to a method for simultaneously detecting protein content and fat content in milk containing particles.
Background
At present, only fat in the particle-containing milk can be detected by a gambling method, but the detection time is about 30 minutes, the accuracy is about +/-15 percent, and the detection of protein in the particle-containing milk is long in time consumption, and dangerous chemical samples such as concentrated sulfuric acid and the like are required to be used, so that a great deal of time is consumed if the fat and the protein in the particle-containing milk are detected simultaneously, the production efficiency is affected, and the inspection work is more dangerous.
Thus, there is a need for a new detection method that can simultaneously and rapidly detect proteins and fats in milk containing particles.
Disclosure of Invention
The invention aims to provide a method for simultaneously detecting the protein content and the fat content in granular milk, which can shorten the detection time and improve the production efficiency.
To achieve the above object, the present invention provides a method for simultaneously detecting protein content and fat content in milk containing particles, comprising the steps of: and (3) temperature adjustment: heating the milk containing the particles to 30-55deg.C to obtain temperature-regulating milk; and (3) filtering: filtering the temperature-regulated milk to obtain filtered milk; and (3) infrared spectrum analysis: and carrying out infrared spectrum analysis on the filtered milk to obtain the content of protein and fat.
According to some embodiments of the invention, the infrared spectrum analysis step comprises the following operations: measuring the absorbance value of the milk containing the particles by a milk component analyzer; the absorbance values are converted into fat content and protein content in the particle-containing milk according to a calibration curve pre-recorded in the milk component analyzer.
According to some embodiments of the invention, the formula according to which the conversion is based is the beer's law formula: a=abc; in the above formula: a=absorbance at a certain wavelength, a=molar absorption coefficient, b=optical path length of the absorption medium, c=sample concentration.
According to some embodiments of the invention, the calibration curve is obtained by: the method comprises the steps of respectively measuring the first fat content and the first protein content of each concentration by a manual method through pre-selecting particle-containing milk with different concentrations; filtering the granular milk with different concentrations respectively to obtain filtered milk, and detecting the filtered milk by using a milk component analyzer to obtain a second fat content and a second protein content of each concentration respectively; and calibrating the first fat content and the first protein content with the second fat content and the second protein content to obtain a calibrated fat curve and a calibrated protein curve.
According to some embodiments of the invention, the milk component analyzer is selected from FT1/FT120 milk component analyzers.
According to some embodiments of the invention, 10-15 different concentrations of the particle-containing milk are selected.
According to some embodiments of the invention, the method for determining fat content by hand is selected from the group consisting of alkaline hydrolysis GB5009.6-2016.
According to some embodiments of the invention, the method for determining the protein content in a manual method is selected from Kjeldahl method GB5009.5-2016.
According to some embodiments of the invention, the filtering step is performed using a filter screen.
According to some embodiments of the invention, the filter screen has a pore size of 300-400 mesh.
The method for simultaneously detecting the protein content and the fat content in the granular milk provided by the invention can shorten the detection time, improve the production efficiency, ensure that production personnel can accurately control the production process, achieve the effect of accurately dosing and thus controlling the production cost, simultaneously lighten the working intensity of inspectors, avoid using dangerous chemical samples such as concentrated sulfuric acid and the like, and ensure that the inspection work becomes safer and easier.
Detailed Description
In order to more clearly understand the technical features, objects and advantages of the present invention, a further detailed description will now be made of the technical scheme of the present invention. It should be understood that the following detailed description is merely exemplary, and the technical solutions of the present invention are not limited to the following detailed description.
The invention aims to provide a method for simultaneously detecting the protein content and the fat content in granular milk, which can enable production staff to accurately control the production process and achieve the effect of accurately dosing so as to control the production cost.
To this end, the invention provides a method for simultaneously detecting the protein content and the fat content of a particle-containing milk, preferably cereal milk, comprising the steps of:
(1) And (3) temperature adjustment: heating the milk containing the particles to 30-55deg.C to obtain temperature-regulating milk;
(2) And (3) filtering: filtering the temperature-regulated milk by using a filter screen with 300-400 meshes (preferably 350 meshes) to obtain filtered milk;
(3) And (3) infrared spectrum analysis: and carrying out infrared spectrum analysis on the filtered milk to obtain the content of protein and fat.
According to one embodiment, the infrared spectrum analysis step comprises the following operations:
(A) Acquisition of calibration fat and calibration protein curves
The method comprises the steps of respectively measuring the first fat content and the first protein content of each concentration by a manual method through pre-selecting 10-15 particle-containing milks with different concentrations;
filtering the 10-15 granular milk with different concentrations respectively to obtain filtered milk, and detecting the filtered milk by using a milk component analyzer to obtain a second fat content and a second protein content of each concentration;
calibrating the first fat content and the first protein content with the second fat content and the second protein content to obtain a calibrated fat curve and a calibrated protein curve, and recording the calibrated fat curve and the calibrated protein curve into a FT1/FT120 milk component analyzer;
(B) Sample loading detection:
measuring the absorbance value of the filtered milk by a milk component analyzer;
(C) The detection result is provided with:
the absorbance values are converted into fat content and protein content in the milk containing particles according to a calibration curve recorded in advance in the milk component analyzer, and the data is read.
According to one embodiment, the formula according to which the transformation is based is the beer's law formula: a=abc; in the above formula: a = absorbance at a certain wavelength; a = molar absorption coefficient; b = optical path of the absorption medium; c = sample concentration.
According to one embodiment, the method for determining the fat content in the above-mentioned manual method is selected from the group consisting of basic hydrolysis methods GB5009.6-2016, and the method for determining the protein content in the above-mentioned manual method is selected from the group consisting of Kjeldahl method GB5009.5-2016.
The method for simultaneously detecting the protein content and the fat content can also simultaneously detect sucrose, full-fat milk solids and the like.
The method for simultaneously detecting the protein content and the fat content in the grain-containing milk has small absolute deviation compared with a manual detection method (fat: alkaline hydrolysis method GB5009.6-2016 third method, protein: kjeldahl nitrogen method GB5009.5-2016 first method: fat is less than or equal to 0.05 percent, protein is less than or equal to 0.03 percent) and high repeatability (when the milk component analyzer is used for detecting the grain-containing milk, the relative standard deviation of the fat and the protein is less than 1 percent, the maximum value-minimum value is less than or equal to 0.03 percent (6 parallel tests)), and the detection efficiency is high (each sample takes 3 minutes).
Example 1
The embodiment provides a method for simultaneously detecting the protein content and the fat content in the milk containing particles, which comprises the following steps:
(1) Calibration curve: selecting 10-15 batches of granular milk with different concentrations, determining the fat content and the protein content by adopting a manual method (fat: alkaline hydrolysis method GB5009.6-2016 third method, protein: kjeldahl nitrogen method GB5009.5-2016 first method), detecting the filtrate after filtering with 350 meshes by using a milk component analyzer (FT 1/FT120 milk component analyzer) to establish a sample set, and calibrating a fat curve and a protein curve;
(2) Sample detection
a) And (3) filtering: preheating 8 groups of samples in Table 1 (30-35 ℃) and pouring the samples into a 350-mesh filter screen for filtering;
b) And (3) detection: and placing the filtered filtrate at a sample inlet of a milk component analyzer (FT 1/FT120 milk component analyzer), selecting a corresponding module, pressing an F9 operation key to start analyzing a sample, and directly reading a detection result on instrument software after the instrument display and the inspection are finished. The test results are shown in Table 1.
TABLE 1
Figure BDA0003359243100000041
As is clear from table 1, the fat and protein contents detected by the milk component analyzer of the present application have small test deviation and high reproducibility as compared with the reference fat and protein contents.
The foregoing is only a preferred embodiment of the present invention. It will be understood that various modifications, combinations, alterations, or substitutions of the details and features of the invention may be made by those skilled in the art without departing from the spirit and nature of the invention. Such modifications, combinations, variations, or alternatives are also to be understood as being included within the scope of the invention as claimed.

Claims (9)

1.一种同时检测含颗粒奶中蛋白质含量和脂肪含量的方法，其特征在于，包括以下步骤：1. A method for simultaneously detecting protein content and fat content in granular milk, characterized in that, comprising the following steps: 调温：将含颗粒奶加热至30-55℃，得调温奶；Tempering: heat the milk containing granules to 30-55°C to obtain tempered milk; 过滤：将所述调温奶进行过滤，得过滤奶；Filtration: filtering the tempered milk to obtain filtered milk; 红外光谱分析：将所述过滤奶进行红外光谱分析，同时得出蛋白质含量和脂肪含量。Infrared spectrum analysis: the filtered milk is subjected to infrared spectrum analysis to obtain protein content and fat content at the same time. 2.根据权利要求1所述的方法，其特征在于，红外光谱分析步骤包括以下操作：2. method according to claim 1, is characterized in that, infrared spectroscopic analysis step comprises the following operations: 通过乳成分分析仪测定含颗粒奶的吸光度值；Determination of the absorbance value of milk containing particles by a milk component analyzer; 根据预先录至乳成分分析仪中的定标校准曲线将所述吸光度值转换成含颗粒奶中的脂肪含量和蛋白质含量。The absorbance values were converted to fat content and protein content in the milk containing particles according to a calibration calibration curve pre-recorded in the milk composition analyzer. 3.根据权利要求2所述的方法，其特征在于，转换所依据的公式为比尔定律公式：A＝abc3. method according to claim 2, is characterized in that, the formula that conversion basis is Beer's law formula: A=abc 上式中：A＝某一波长的吸光度In the above formula: A = absorbance at a certain wavelength a＝摩尔吸光系数a = molar absorptivity b＝吸收介质的光程b = optical path of the absorbing medium c＝样品浓度。c = sample concentration. 4.根据权利要求2所述的方法，其特征在于，所述定标校准曲线是通过如下方式获得的：4. The method according to claim 2, wherein the calibration calibration curve is obtained as follows: 通过预先选取不同浓度的含颗粒奶，用手工法分别测定出各浓度的第一脂肪含量和第一蛋白质含量；The first fat content and the first protein content of each concentration are measured respectively by manual method by pre-selecting different concentrations of grain-containing milk; 将所述不同浓度的含颗粒奶分别过滤后得到过滤奶，然后将所述过滤奶使用乳成分分析仪检测分别获得各浓度的第二脂肪含量和第二蛋白质含量；Filter the milk containing particles of different concentrations to obtain filtered milk, and then use a milk component analyzer to detect the filtered milk to obtain the second fat content and the second protein content of each concentration; 将所述第一脂肪含量和第一蛋白质含量与所述第二脂肪含量和第二蛋白质含量进行校准，获得校准脂肪曲线和校准蛋白质曲线。The first fat content and the first protein content are calibrated with the second fat content and the second protein content to obtain a calibration fat curve and a calibration protein curve. 5.根据权利要求2所述的方法，其特征在于，所述乳成分分析仪选自FT1/FT120乳成分分析仪。5. The method according to claim 2, wherein the milk component analyzer is selected from FT1/FT120 milk component analyzer. 6.根据权利要求4所述的方法，其特征在于，选取10-15个不同浓度的含颗粒奶。6. The method according to claim 4, characterized in that 10-15 grain-containing milks of different concentrations are selected. 7.根据权利要求4所述的方法，其特征在于，手工法中的脂肪含量测定方法选自碱水解法GB5009.6-2016。7. The method according to claim 4, characterized in that the fat content determination method in the manual method is selected from the alkali hydrolysis method GB5009.6-2016. 8.根据权利要求4所述的方法，其特征在于，手工法中蛋白质含量的测定方法选自凯氏定氮法GB5009.5-2016。8. The method according to claim 4, characterized in that, the assay method of protein content in manual method is selected from Kjeldahl method GB5009.5-2016. 9.根据权利要求1所述的方法，其特征在于，过滤步骤中，采用过滤网进行过滤，所述过滤网的孔径为300-400目。9. The method according to claim 1, characterized in that, in the filtering step, a filter screen is used for filtering, and the aperture of the filter screen is 300-400 mesh.
CN202111360944.6A 2021-11-17 2021-11-17 Method for Simultaneously Detecting Protein Content and Fat Content in Grain-Containing Milk Pending CN116136495A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258620A (en) * 1990-06-15 1993-11-02 Snow Brand Milk Products Co., Ltd. Method and apparatus for determining the constituents of dairy products
US20040135088A1 (en) * 2001-03-16 2004-07-15 Lars-Ove Sjaunja Mid infra red analysis
EP2418474A1 (en) * 2010-08-02 2012-02-15 OOO "Novye Energeticheskie Technologii" Near infrared spectroscopy method for simultaneously determining the components of raw milk
CN102435580A (en) * 2011-12-23 2012-05-02 吉林大学 Quick near-infrared measuring device and method for milk ingredients
CN107655852A (en) * 2017-09-29 2018-02-02 广东出入境检验检疫局检验检疫技术中心 The near infrared spectrum quick determination method of essential nutrient in baby formula milk powder
CN211122522U (en) * 2019-12-05 2020-07-28 南京先进激光技术研究院 Device for rapid identification of fat and protein content in raw milk based on infrared spectroscopy

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258620A (en) * 1990-06-15 1993-11-02 Snow Brand Milk Products Co., Ltd. Method and apparatus for determining the constituents of dairy products
US20040135088A1 (en) * 2001-03-16 2004-07-15 Lars-Ove Sjaunja Mid infra red analysis
EP2418474A1 (en) * 2010-08-02 2012-02-15 OOO "Novye Energeticheskie Technologii" Near infrared spectroscopy method for simultaneously determining the components of raw milk
CN102435580A (en) * 2011-12-23 2012-05-02 吉林大学 Quick near-infrared measuring device and method for milk ingredients
CN107655852A (en) * 2017-09-29 2018-02-02 广东出入境检验检疫局检验检疫技术中心 The near infrared spectrum quick determination method of essential nutrient in baby formula milk powder
CN211122522U (en) * 2019-12-05 2020-07-28 南京先进激光技术研究院 Device for rapid identification of fat and protein content in raw milk based on infrared spectroscopy

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