CN116077405A - Anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and its preparation process - Google Patents

Abstract

The invention discloses an anti-desquamation shampoo containing saccharomyces cerevisiae protein hydrolysate, which is prepared from five phases of A, B, C, D and E, wherein the phase A at least comprises water and a cleaning component, the phase B at least comprises a desquamation component, the phase C at least comprises an antipruritic component, the phase D at least comprises a plant extract, and the phase E at least comprises a protein component. The invention adopts the synergistic effect of the myristamide propyl PG-dimethyl ammonium chloride phosphate and the piroctone olamine salt, can effectively inhibit malassezia and improve the antibacterial rate, thereby effectively radically treating dandruff and relieving itching, is different from the traditional product which temporarily eliminates dandruff in a degreasing way, and has long-acting property in removing dandruff. And the shampoo can produce better oil control and moisture preservation effects, reduce the irritation of salicylic acid to skin mucous membrane and eye mucous membrane, improve the alopecia effect of the shampoo, reduce the problem of hair loss in the shampoo process, and increase the hair density.

Description

Anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and its preparation process

technical field

The invention relates to an anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and a preparation process thereof, and relates to A61K, in particular to the field of makeup preparations.

Background technique

With the development of society, people's life pressure is increasing, and hair loss is becoming more and more common. Most of the hair loss problems caused by stress and hormone secretion disorders are becoming more and more common. In addition to stress hair loss, unreasonable use of shampoo has further aggravated the problem of hair loss. Along with hair loss, dandruff and itching also plague most people. At present, most anti-dandruff shampoos on the market temporarily eliminate dandruff from the appearance by degreasing. Once stopped, severe dandruff will occur again, and long-term use of degreasing to remove dandruff will cause scalp sensitivity , destroy the skin barrier. Therefore it is very important to develop a kind of anti-dandruff antipruritic shampoo with anti-shedding effect.

Chinese invention patent CN202210798060.7 discloses an anti-hair loss, anti-dandruff and anti-itch plant shampoo and its preparation method, by introducing plant combination extract, cocamidopropyl PG-dimethyl ammonium chloride phosphate, The plant shampoo prepared by the synergistic action of piroctone olamine is safe and mild, and has the effects of preventing hair loss, strengthening hair, removing dandruff and relieving itching, but can only achieve short-term anti-dandruff effect, and cannot achieve long-term anti-dandruff. Chinese invention patent CN201710952333.8 discloses a traditional Chinese medicine shampoo powder for anti-dandruff, anti-pruritic and anti-hair loss and its preparation method. By grinding a variety of Chinese medicinal plants into powder, according to the matching of traditional Chinese medicine, it can dispel wind, activate blood circulation, dredge meridians, Nourishes blood and moisturizes hair, anti-allergic, sterilizing and decontaminating effects, but the prepared shampoo powder is inconvenient to use, and users need to configure it into a solution for use, and the steps are cumbersome.

Contents of the invention

In order to improve the anti-dandruff and anti-itch effect of the shampoo, and maintain the long-term anti-dandruff effect, the first aspect of the present invention provides an anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate, the preparation raw materials include: A, B, C, D, E five phases, the A phase at least includes water and cleansing components, the B phase includes at least an anti-dandruff component, the C phase includes at least an antipruritic component, and the D phase It includes at least plant extracts, and the E phase includes at least protein components; water supplements the total mass of the shampoo to 100%.

As a preferred embodiment, the A phase also includes 0.1-1% of conditioning agent, 5-60% of cleaning component, 0.1-1% of moisturizing agent, 0.1-2% of thickener, emulsifier 0.1-2%, pH regulator 0.05-0.3%, softener 0.05-0.5%.

As a preferred embodiment, the cleaning component is selected from one or a combination of anionic surfactants, cationic surfactants, nonionic surfactants, and zwitterionic surfactants.

As a preferred embodiment, the zwitterionic surfactant is selected from one of amino acid amphoteric surfactants or betaine amphoteric surfactants, and the substituents in the zwitterionic surfactant include cocoamide substitution base.

As a preferred embodiment, the cleaning component is selected from sodium lauryl sulfate, sodium dodecylbenzenesulfonate, sodium laureth sulfate, sodium α-alkenylsulfonate, imidazoline betaine, In Coconut Diethanolamide, Cocamidopropyl Betaine, Lauryl Dimethyl Betaine, Lauryl Dimethyl Amine Oxide, Alkyl Glucosides, Sodium Methyl Cocoyl Taurate one or a combination of several.

As a preferred embodiment, the cleaning component is a combination of anionic, nonionic and zwitterionic surfactants.

As a preferred embodiment, the weight ratio of the anionic, nonionic and zwitterionic surfactants is (5-40):(0.5-5):(1-15).

As a preferred embodiment, the cleaning component is a combination of sodium laureth sulfate, cocamidopropyl betaine, alkyl glucoside, and sodium methyl cocoyl taurate.

As a preferred embodiment, the sodium laureth sulfate, alkyl glucoside, cocamidopropyl betaine, and the weight ratio of sodium methyl cocoyl taurate are (5-40): (0.5-5): (1-10): (0.5-3).

During the experiment, the applicant found that the synergistic effect of anionic, nonionic and zwitterionic surfactants can make the shampoo have a good cleaning effect, and form delicate and stable foam during the cleaning process, which is easy to rinse. The reason is that anionic, nonionic and zwitterionic surfactants are synergistic, which can increase the cleaning power of surfactants. The dense foam formed during use can quickly moisten the hair, combine with the oil on the hair surface, and take away the oil on the hair surface after washing. , to achieve a good cleaning effect. The applicant further found that the weight ratio of (5-40):(0.5-5):(1-15) for anionic, nonionic and zwitterionic surfactants can make the shampoo have good storage stability and make the side Cypress leaf extract, Sophora flavescens extract and hydrolyzed yeast protein can be uniformly dispersed in the solvent to form a stable emulsified system.

As a preferred embodiment, the thickener is selected from one or a combination of sodium chloride, ammonium chloride, carbomer, xanthan gum, and acrylate copolymers.

As a preferred embodiment, the conditioner is selected from polydimethylsiloxane, polyquaternium-7, polyquaternium-39, guar hydroxypropyltrimethylammonium chloride, polyquaternium One or more combinations of quaternary ammonium salts-10.

As a preferred embodiment, the conditioner is a combination of guar hydroxypropyltrimethylammonium chloride and polyquaternium-10.

As a preferred embodiment, the weight ratio of guar hydroxypropyltrimethylammonium chloride to polyquaternium-10 is (0.05-0.5):(0.05-0.5).

As a preferred embodiment, the humectant is PEG-7 glyceryl cocoate. The thickener is cocamide methyl MEA. The emulsifier is myristamidopropyl PG-dimethylammonium chloride phosphate. The pH regulator is citric acid. Described softening agent is stearamidopropyl dimethylamine.

As a preferred embodiment, the phase B includes 0.1-0.5% of stabilizer I, 0.1-2% of anti-dandruff component, 0.1-1% of panthenol and 0.5-1% of zinc salt in weight percentage.

As a preferred embodiment, the anti-dandruff component is piroctone olamine salt and salicylic acid.

As a preferred embodiment, the weight ratio of the piroctone olamine salt to salicylic acid is (0.1-1):(0.05-1).

During the experiment, the applicant found that the synergistic effect of myrismidopropyl PG-dimethylammonium chloride phosphate and piroctone olamine salt can significantly improve the anti-dandruff and anti-itch effect of the shampoo, and achieve long-acting anti-dandruff. Scabs relieve itching. The possible reason is: the solubility of piroctone olamine salt in water is not good, and it is not easy to add too much in the shampoo with water as the solvent, resulting in poor anti-dandruff effect. Base PG-dimethylammonium chloride phosphate and piroctone olamine salt synergistically, can increase the solubility of piroctone olamine salt in water solvent, increase its addition amount, increase piroctone olamine salt in shampoo Increase the amount of deposition in the system, thereby inhibiting the reproduction of Malassezia, improving the antibacterial rate of shampoo, increasing the deposition amount, enhancing the anti-dandruff effect, fundamentally blocking the external channels of dandruff production, and eradicating dandruff and itching.

As a preferred embodiment, the zinc salt is a combination of zinc PCA and zinc gluconate.

As a preferred embodiment, the weight ratio of zinc PCA to zinc gluconate is (0.05-0.5): (0.005-0.5).

During the experiment, the applicant found that the introduction of salicylic acid into the shampoo can clean the excessively secreted oil on the surface of the scalp and has a good oil control effect, but long-term use of salicylic acid will excessively remove the cuticle, resulting in a decrease in the defense ability of the scalp. Cause stinging or allergy problems. By introducing salicylic acid, betaine salicylate and PCA zinc, the applicant can reduce the irritation of the shampoo to the skin and mucous membranes of the scalp, and it will not be too irritating after accidentally entering the eyes. Guess the possible reason Yes: The introduction of betaine salicylate can neutralize part of the acidity of salicylic acid, reduce the acid irritation of salicylic acid, and the presence of PCA zinc can bind water, keep the cuticle of the scalp in a hydrated state, and maintain the barrier Healthy, further reducing the irritation of salicylic acid to the skin. The applicant further found that the introduction of PCA zinc can also inhibit the activity of 5α-reductase and the conversion of androgen, thereby reducing sebum secretion and achieving oil control and antibacterial effects.

As a preferred embodiment, the phase C includes 0.1-1% antipruritic component and 0.01-0.5% amino acid in weight percentage.

As a preferred embodiment, the antipruritic component is betaine salicylate, and the amino acid is arginine.

As a preferred embodiment, the D phase includes 0.3-0.8% of stabilizer II, 0.00005-0.2% of vitamins, 0.1-1% of consistency regulator, 0.5-1% of essence, and 0.2% of plant extract in terms of weight percentage. -3%.

As a preferred embodiment, the stabilizer I is sodium benzoate, and the stabilizer II is phenoxyethanol. The vitamin is vitamin H, commonly known as biotin. The consistency regulator is sodium chloride.

As a preferred embodiment, the plant extract is selected from one or more of the extracts of oriental cypress extracts, Sophora flavescens extracts, ginger root extracts, angelica extracts, Polygonum multiflorum extracts, Sapindus extracts The combination.

As a preferred embodiment, the plant extract is a combination of Platycladus orientalis (L.) Franco leaf extract and Sophoraflavescens extract.

As a preferred embodiment, the weight ratio of the cypress leaf extract to the Sophora flavescens extract is (0.1-2): (0.1-1).

As a preferred embodiment, the E phase includes 0.0001-0.5% of protein components in weight percentage, and the protein components are hydrolyzed protein components.

As a preferred embodiment, the protein component is hydrolyzed yeast protein, which is a protein hydrolyzate of yeast used for brewing fermentation, and consists of 16 kinds of amino acids.

The second aspect of the present invention provides a preparation process of anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate, comprising the following steps:

(1) Add the components of phase A into the stirring pot, stir and heat to 80-90°C, keep for 20-40min, until all the substances of phase A are dissolved;

(2) Cool down the stirring pot to 55-65°C, add phase B, stir and mix;

(3) Cool down the stirring pot to 50-60°C, add phase C, stir and mix;

(4) Cool down the stirring pot to 40-50°C, add phase D, stir and mix;

(5) Cool down the stirring pot to 35-45°C, add phase E, stir and mix;

(6) Cool down to 35-40°C, control the pH to 5-6, and get the product after discharging.

As a preferred embodiment, step 6 controls the pH to be 5.2-5.7.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The anti-dandruff shampoo of the present invention adopts myristamidopropyl PG-dimethylammonium chloride phosphate and piroctone ethanolamine salt to act synergistically, which can effectively inhibit Malassezia and improve Antibacterial rate, so as to effectively cure dandruff and relieve itching. Unlike traditional products that temporarily eliminate dandruff by degreasing, anti-dandruff has long-term effect.

(2) Anti-shedding and anti-dandruff shampoo of the present invention adopts salicylic acid, betaine salicylate and PCA zinc to act synergistically, and adopts (0.05-1): (0.1-1): (0.05-0.5 ) weight ratio, can produce better oil control and moisturizing effect, and reduce the irritation of salicylic acid to skin mucous membranes and eye mucous membranes.

(3) The anti-shedding and anti-dandruff shampoo of the present invention adopts the combination of anionic, nonionic and amphoteric surfactants as the cleaning component, which can make the shampoo produce delicate, stable and rich foam, with excellent cleaning effect. The prepared The shampoo has good stability, it is not easy to separate under high and low temperature environment, and it can be stored for a long time.

(4) The anti-shedding and anti-dandruff shampoo of the present invention introduces cypress leaf extract, Sophora flavescens extract, and hydrolyzed yeast protein into D phase and E phase, wherein, hydrolyzed yeast protein acts on the seven hair follicles Target proteins (keratin 71, hyalin, keratin 14, keratin 17, collagen IV, collagen I, CD34), comprehensively protect hair follicle tissue, synergistically improve the anti-hair loss effect of shampoo, reduce shampooing Processes hair fall problems and increases hair density.

(5) The anti-shedding and anti-dandruff shampoo of the present invention has better oil control effect, can fully relieve scalp inflammation, and shape the healthy state of hair, especially for young men with strong oil secretion, the effect is remarkable.

Description of drawings

Fig. 1-3 is the test diagram of the inhibition zone of three parallel experiments of embodiment 1.

Detailed ways

Example 1

An anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate, the preparation raw materials include five phases A, B, C, D and E.

In percent by weight: said phase A also includes 0.5% conditioner, 40% cleansing component, 0.5% moisturizer, 1% thickener, 1.2% emulsifier, 0.15% pH regulator, 0.26% softener, water Replenishes the total mass of shampoo to 100%.

The conditioner is a combination of guar gum hydroxypropyltrimethylammonium chloride and polyquaternium-10, the weight ratio is 0.1:0.25, and the polyquaternium-10 is purchased from Shanghai Yuanye Biotechnology Co., Ltd. Company, CAS No.: 68610-92-4. The cleaning component is a combination of sodium laureth sulfate, cocamidopropyl betaine, decyl glucoside and sodium methyl cocoyl taurate, and the weight ratio is 30:2.5:5:1.5.

The humectant is PEG-7 glyceryl cocoate. The thickener is cocamide methyl MEA. The emulsifier is myristamidopropyl PG-dimethylammonium chloride phosphate. The pH regulator is citric acid. Described softening agent is stearamidopropyl dimethylamine.

The phase B includes 0.1% of stabilizer I, 1.5% of anti-dandruff component, 0.5% of panthenol and 0.75% of zinc salt.

The anti-dandruff component is piroctone olamine salt and salicylic acid, and the weight ratio is 0.5:0.55.

The zinc salt is a combination of zinc PCA and zinc gluconate, with a weight ratio of 0.25:0.1.

The C phase comprises antipruritic components 0.5% and amino acids 0.03%.

The antipruritic component is betaine salicylate, and the amino acid is arginine.

The D phase includes 0.3% of stabilizer II, 0.1% of vitamins, 0.1% of consistency regulator, 0.5% of essence and 1.5% of plant extract.

The stabilizer I is sodium benzoate, and the stabilizer II is phenoxyethanol. The vitamin is biotin. The consistency regulator is sodium chloride.

The plant extract is a combination of arborvitae leaf extract and Sophora flavescens extract, with a weight ratio of 1.2:0.5.

The E phase includes 0.25% of protein components, and the protein components are hydrolyzed yeast proteins.

A preparation process of anti-shedding and anti-dandruff shampoo, comprising the following steps:

(1) Add the components of phase A into the stirring pot, stir and heat to 85°C, and keep for 30 minutes until all the substances of phase A are dissolved;

(2) Cool down the stirring pot to 60°C, add phase B, stir and mix;

(3) Cool down the stirring pot to 55°C, add phase C, and stir to mix;

(4) Cool down the stirring pot to 45°C, add phase D, stir and mix;

(5) Cool the stirring pot to 40°C, add phase E, and stir to mix;

(6) Cool down to 38°C, control the pH to 5.5, and get the product after discharging.

Example 2

An anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and its preparation process, the specific steps are the same as in Example 1, the difference is that the emulsifier is cocamidopropyl PG-dimethyl ammonium chloride phosphoric acid The ester, the anti-dandruff component is piroctone olamine salt, the mass fraction is 1%.

Example 3

An anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and its preparation process, the specific steps are the same as in Example 1, the difference is that the anti-dandruff components are piroctone olamine salt and salicylic acid, weight The ratio is 0.5:1. The zinc salt is zinc gluconate.

Example 4

An anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate and its preparation process, the specific steps are the same as in Example 1, the difference is that the cleaning components are sodium laureth sulfate, cocamidopropyl Combination of betaine, lauryl glucoside, sodium methyl cocoyl taurate in a weight ratio of 26:3:8:2.

Performance Testing

1. Bacteriostasis test: According to the QW66-inhibition zone test guide, three parallel antibacterial performance tests were carried out on the shampoo of Example 1, and A～I are 9 parallel samples. The larger the antibacterial diameter D (mm), the better the antibacterial effect, and the better the anti-dandruff effect; see Table 1 for the antibacterial circle table. See Figure 1-3 for pictures of the inhibition zone.

Table 1

The measured diameter of each antibacterial hole is greater than 7mm, which has antibacterial ability. The mixture of shampoo and water with a mass concentration of 10% has the effect of inhibiting Malassezia.

2. Volunteers' use experience test: Volunteers who live in a test group use the shampoo prepared in Example 1, and the number of people who complete the questionnaire is 30. Test the user experience (aroma, foam), product efficacy (dandruff control, oil control, itching relief, suppleness), sensitivity (itchy head, small bumps, dandruff, tight scalp, red scalp).

See Table 2 for the experience test. The flavor effect test is shown in Table 3. See Table 4 for foam volume effect test.

Table 2

number of people Anti-dandruff effect oil control effect Antipruritic effect smooth effect satisfied/person 16 16 20 9 normal person 14 13 10 twenty one dissatisfied/person 0 1 0 0

table 3

like it very much Kind of like generally I don't like it very dislike Fragrance effect/person 7 9 13 1 0

Table 4

Much a little too much Moderate a little less very few Foam volume/person 5 6 18 1 0

3. Anti-hair loss effect test: select 10 healthy adults aged 18-60, with hair length between 5-40cm: those who suffer from frequent hair loss and mild thinning of hair. And according to 60 hair combing methods, the hair loss count is more than 10, and the hair loss is still more than 10 after the 2-week wash-out period, and has not undergone hair dyeing, perm, or special hair styling treatment in the past 1 month.

Local hair density: Fix a 1.5cm*1.5cm hair-cutting area on the subject’s head, and position it well. At each visit, ensure that the hair-cutting area is consistent, and the hair is cut until the residual length does not exceed 1mm: Image acquisition During the process, the operator put the subject in a comfortable position, placed the dermatoscope in the center of the hair-cutting area to take partial hair image shooting, and the dermatoscope lens was completely attached to the scalp and kept vertical when shooting, and the captured image was checked clarity. By manual counting method, the number (root) and density (root/cm ² ) of local hairs were counted and recorded.

Before use, the subject sits quietly for 30min in a laboratory with a temperature of 21°C and a humidity of 50%, performs 60 times of hair combing, and records the amount of hair loss. After using the shampoo of Example 1 for 4 weeks and 8 weeks, the subjects were respectively Sit quietly for 30 minutes in a laboratory with a temperature of 21° C. and a humidity of 50%, perform 60 combing methods, and record the number of hair loss. The test results are shown in Table 5.

table 5

60 hair combing method: the subject cannot wash his hair within 48#4 hours before each visit. No hair combing on the morning of the visit: after acclimatization at the testing facility each time. The trained technicians use combs of the same specifications to comb 60 times at a constant speed (30 times each for left and right) in the order from front left to back left, front right to back right, collect and count the fallen hairs.

The test results showed that after 4 and 8 weeks of use, the amount of hair loss decreased or changed little, and the hair density increased or changed little.

4. Stability test: place the shampoo in an environment of -10°C and 55°C for 3 months, observe the apparent state of Examples 1-4, and observe whether there is delamination or floc problems. The test results are shown in Table 6.

Table 6

-10°C 55°C Example 1 No delamination, no flocculent turbidity No delamination, no flocculent turbidity Example 2 No delamination, flocs appear No delamination, no flocculent turbidity Example 3 No delamination, no flocculent turbidity No delamination, no flocculent turbidity Example 4 No delamination, no flocculent turbidity No delamination, no flocculent turbidity

Claims (10)

1. An anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate, is characterized in that, preparation raw material comprises A, B, C, D, E five phases, and described A phase comprises water and cleaning component at least, The phase B includes at least an anti-dandruff component, the phase C includes at least an antipruritic component, the phase D includes at least a plant extract, and the phase E includes at least a protein component; Quality to 100%. 2. The anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to claim 1, characterized in that, the A phase also includes 0.1-1% of conditioning agent and 5-60% of cleaning components by weight percentage. %, moisturizing agent 0.1-1%, thickener 0.1-2%, emulsifier 0.1-2%, pH regulator 0.05-0.3%, softener 0.05-0.5%. 3. the anti-dandruff shampoo containing saccharomyces cerevisiae protein hydrolyzate according to claim 2, is characterized in that, described cleaning component is selected from anionic surfactant, cationic surfactant, nonionic surfactant, One or a combination of zwitterionic surfactants. 4. the anti-dandruff shampoo containing saccharomyces cerevisiae protein hydrolyzate according to claim 3, is characterized in that, described zwitterionic surfactant is selected from amino acid amphoteric surfactant or betaine amphoteric surfactant One, the substituent in the zwitterionic surfactant comprises a cocoamide substituent. 5. The anti-dandruff and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to claim 1, is characterized in that, described B phase comprises stabilizing agent I 0.1-0.5% by weight percentage, anti-dandruff component 0.1% -2%, panthenol 0.1-1% and zinc salts 0.5-1%. 6. The anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to claim 1, characterized in that, the C phase includes 0.1-1% antipruritic component and 0.01-0.5% amino acid in weight percentage . 7. The anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to claim 1, characterized in that, the D phase includes stabilizer II 0.3-0.8% and vitamin 0.00005-0.2% by weight percentage , Consistency Regulator 0.1-1%, Flavor 0.5-1%, Plant Extract 0.2-3%. 8. the anti-shedding and anti-dandruff shampoo containing saccharomyces cerevisiae protein hydrolyzate according to claim 7, is characterized in that, said plant extract is selected from the group consisting of arborvitae extract, Sophora flavescens extract, ginger root extract, angelica extract, Polygonum multiflorum extract, Sapindus extract, or a combination of several. 9. The anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to claim 1, characterized in that, the E phase comprises 0.0001-0.5% of the protein components by weight percentage, and the protein components Divided into protein components after hydrolysis, preferably the protein hydrolyzate of yeast used for brewing fermentation. 10. A preparation process for anti-shedding and anti-dandruff shampoo containing Saccharomyces cerevisiae protein hydrolyzate according to any one of claims 1-9, characterized in that it comprises the following steps: (1) Add the components of phase A into the stirring pot, stir and heat to 80-90°C, keep for 20-40min, until all the substances of phase A are dissolved; (2) Cool down the stirring pot to 55-65°C, add phase B, stir and mix; (3) Cool down the stirring pot to 50-60°C, add phase C, stir and mix; (4) Cool down the stirring pot to 40-50°C, add phase D, stir and mix; (5) Cool down the stirring pot to 35-45°C, add phase E, stir and mix; (6) Cool down to 35-40°C, control the pH to 5-6, and get the product after discharging.

Images