CN116035992A - Composition with hair growth and fixation function, preparation method and application - Google Patents

Abstract

The invention discloses a composition with functions of growing and fixing hair, a preparation method and application, and belongs to the technical field of growing and fixing hair. The composition comprises the following raw materials in parts by weight: the main components comprise 1020 to 3050 parts of protein and 22.4 to 140 parts of nutrition enhancer; the functional components comprise 20-200 parts of powdered plant sterol ester, 100-500 parts of millet extract, 100-500 parts of rhizoma polygonati powder, 100-400 parts of mulberry powder and 100-500 parts of Aronia melanocarpa fruit powder; auxiliary ingredients, including 200-1800 parts of flavoring agent. The composition has no toxic or side effect, can promote metabolism and proliferation of hair follicle cells, can effectively promote formation of hair follicle melanin, shortens the time of hair follicle entering into growing period from resting period and growing hair, and can strengthen the tightness of hair in scalp, thereby achieving the effects of preventing hair loss and fixing hair.

Description

Composition with hair growth and fixation function, preparation method and application

Technical Field

The present invention belongs to the technical field of hair growth and fixation, and specifically relates to a composition with hair growth and fixation functions, a preparation method and application thereof.

Background Art

According to statistics, hair loss is becoming more and more common and is occurring at younger ages.

There are many products on the market that claim to have the function of hair growth and hair fixation. However, these products are not very ideal in terms of hair growth and hair fixation effects. Moreover, most of the hair growth products are shampoo and conditioner products, and other products are relatively few. However, since shampoo and conditioner products need to have a cleaning function, it is inevitable to add surfactants, conditioners and other chemical ingredients that may damage the hair. Therefore, there is an urgent need for a composition with good hair growth and hair fixation effects and a hair growth and hair fixation function.

Summary of the invention

In order to solve the above problems in the prior art, the present invention provides a composition with hair growth and fixation function, a preparation method and application thereof.

To achieve the above object, the present invention provides the following technical solutions:

One of the technical solutions of the present invention: a composition with the function of hair growth and fixation, wherein the raw materials of the composition with the function of hair growth and fixation include, by weight:

Furthermore, the protein includes small molecule peptides and amino acids; the small molecule peptides include 500-1500 parts of bovine collagen peptides and 500-1500 parts of hydrolyzed collagen peptides by weight; and the amino acids include 20-50 parts of taurine by weight.

Furthermore, the nutritional enhancers are vitamin enhancers and mineral enhancers.

Furthermore, the vitamin enhancer includes 0.2-10 parts of vitamin A, 10-50 parts of vitamin C, 1-10 parts of vitamin E and 10-50 parts of yeast powder by weight; the mineral enhancer includes 1-10 parts of ferrous fumarate and 0.2-10 parts of zinc oxide by weight.

Furthermore, the powdered phytosterol ester is β-sitosterol ester, and the chemical formula is C ₄₇ O ₂ H ₈₀ .

Furthermore, the preparation method of the millet extract is: crushing the millet, adding water to extract, concentrating the extract, and freeze-drying to obtain the millet extract.

Furthermore, the flavoring agent comprises 100 to 500 parts of erythritol and 100 to 500 parts of grapefruit powder by weight.

The second technical solution of the present invention: The preparation method of the above-mentioned composition having the function of hair growth and fixation comprises the following steps:

(1) Weigh each raw material by weight, sieve and set aside;

(2) mixing the nutritional enhancer and flavoring agent weighed in step (1) to obtain a mixed raw material A;

(3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, and then fluidized bed drying and granulating with a granulator to obtain a mixed raw material B;

(4) Mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3), and sieving them to obtain the composition having the function of hair growth and fixation.

Furthermore, in step (1), the sieving is through a 40-100 mesh sieve.

Furthermore, in step (2), the mixing is equal and incremental mixing.

Furthermore, in step (3), the stirring speed is 200 to 500 rpm; the alcohol dosage is 280 to 480 parts by weight of 70 to 95% alcohol sprayed within 2 to 10 minutes; the fluidized bed drying temperature is 51 to 58° C., and the drying time is 25 to 35 minutes; and the granulator is a 20-mesh granulator.

Furthermore, in step (4), the sieving is through a 40-100 mesh sieve.

The third technical solution of the present invention: application of the above-mentioned composition with hair growth and hair fixation function in hair growth and hair fixation.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The composition with hair growth and fixation function prepared by the present invention can shorten the time for hair follicles to enter the growth phase from the resting phase, accelerate the time for hair to grow, and has a significant promoting effect on hair growth;

(2) The composition with hair growth and hair fixation function prepared by the present invention has no toxic side effects and can effectively promote the regeneration of hair follicles;

(3) The present invention adopts fluidized bed granulation instead of oven drying. Compared with oven drying, the present invention has the advantages of uniform flow state, uniform drying, less damage to the surface of the material, and looser powder; high thermal efficiency and good energy-saving effect, which can save 30-60% energy compared with oven drying.

The hydrolyzed collagen peptide added in the present invention is a necessary component of human skin, which can effectively ensure the moisture of the skin and increase the firmness of the skin. The main protein in hair is keratin, and collagen may support and increase the hair growth protein in the body. This can help strengthen hair, promote hair growth, and prevent hair loss;

In addition, the present application selects two types of collagen peptides because one is a collagen peptide extracted from animal skins, and the other is a collagen peptide derived from bones, which have different amino acid compositions and peptide segments and can complement each other;

The taurine added in the present invention not only has the effect of repairing damaged hair, but also has the effect of increasing the production of hair growth factor and promoting hair growth. Taurine can increase the production of growth factor IGF-1, which can act on the human hair cycle, prolong the growth period, and produce thick and strong hair;

The multivitamins added in the present invention can increase the nutrition of the scalp, promote the metabolism of hair follicle cells, and thus promote the growth of hair;

The yeast powder added in the present invention contains vitamin H and nicotinic acid. The vitamin H contained therein is also called biotin, which is a nutrient necessary for maintaining the natural growth, development and normal function of the human body. It can directly regulate the metabolism of hair follicle cells and can also indirectly affect the development of hair follicles by affecting the food intake or metabolism.

In addition, the niacin contained in yeast powder is also an indispensable nutrient for the human body. It plays an important role in promoting the normal growth and development of the human body. It can promote iron absorption and blood cell production and maintain the normal function of the skin.

The present invention adds ferrous fumarate, which provides oxygen to every cell in the body, including the cells in the scalp and hair follicles;

The present invention adds zinc oxide, which is a cofactor of multiple enzymes in the body and an important component of antioxidant enzymes in the body. It can enhance the activity of blood cells and is a trace element necessary for hair growth and keratin synthesis.

The powdered phytosterol ester added in the present invention is prepared by esterification reaction or transesterification reaction of phytosterol and fatty acid. Sterol ester can be converted into sterol and fatty acid in the human body, so its physiological function includes the physiological functions of phytosterol and fatty acid. Phytosterol ester is more easily absorbed by the human body, and its use efficiency is much higher than that of ordinary phytosterol, and it can prevent hair loss by inhibiting the production of androgen;

The millet extract added in the present invention is a rare oil extracted from millet, specifically millet extract (chemical formula: C ₃₁ H ₅₂ O). The rare oil acts on the roots of hair follicles, can accelerate cell metabolism and proliferation, keep hair growing, nourish and repair damaged scalp, create good basic conditions for healthy hair quality, and also has anti-inflammatory and anti-toxic effects;

The polygonatum powder added in the present invention belongs to the kidney meridian, has the effect of replenishing essence and filling marrow, and has a good effect on treating kidney deficiency type hair loss and the like;

The mulberry powder added in the present invention has the effects of nourishing the kidney and replenishing the essence, and promoting hair growth, and is suitable for treating symptoms of premature aging, premature graying of hair, hair loss, alopecia areata, and hair withering; mulberry is a material in the catalog of medicine and food homology, and according to the pharmacopoeia, it has the effect of treating premature graying of hair; mulberry is rich in proanthocyanidins, and proanthocyanidins may activate stem cells in the bulge of hair follicles, induce the transformation of resting hair follicles to growth phase, and promote hair regeneration;

The aronia melanocarpa fruit powder added by the invention is rich in various vitamins, antioxidant components and mineral elements, and has the effect of improving the blood circulation of the head.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

FIG1 is a diagram showing the changes in the back hair of mice treated with sample 3#;

FIG2 is a diagram showing the changes in the back hair of mice administered with a low-dose sample;

FIG3 is a diagram showing the changes in the back hair of mice administered with a high-dose sample;

FIG4 is a diagram showing the changes in the back hair of mice administered with a positive control sample;

FIG5 is a diagram showing the changes in the back hair of mice administered with a negative control sample;

FIG6 is a statistical diagram of the number of newly formed hair follicles under a microscope for the drug-administered sample 3#, the low-dose sample, the high-dose sample, the positive control sample, and the negative control sample;

Figure 7 is a HE staining image of the administration sample 3#, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

FIG8 is a HE staining image of a low-dose sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

FIG9 is a HE staining image of a high-dose sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

Figure 10 is a HE staining image of the administration positive control sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

Figure 11 is the HE staining image of the negative control sample, wherein (a) is the HE staining image of the mouse skin histology at 7 days, (b) is the HE staining image of the mouse skin histology at 14 days, (c) is the HE staining image of the mouse skin histology at 28 days, and (d) is the HE staining image of the mouse skin histology at 56 days.

DETAILED DESCRIPTION

Now, various exemplary embodiments of the present invention are described in detail, which should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

In addition, for the numerical range in the present invention, it is understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and/or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

The “parts” in the embodiments of the present invention are “parts by weight” unless otherwise specified.

In the following examples, the powdered phytosterol esters were prepared according to patent CN201410445798.0;

In the following examples, the preparation method of the millet extract is:

1) Dry the millet in a hot air drying oven at 37°C for 24 hours and grind it through an 80-mesh sieve;

2) vortex extraction of the sieved millet in step 1) with water at a solid-liquid ratio of 1:10 at 50° C. for 1 h, and suction filtration, and repeating twice, and collecting the filtrate and the filter residue respectively;

3) Concentrating the filtrate obtained in step 2) to a solid content of 25-35% to obtain a concentrated solution;

4) freeze-drying the concentrated solution obtained in step 3) to obtain the millet extract, wherein the freeze-drying parameter is to keep the moisture content below 6%;

The mulberry powder, Aronia nigra fruit powder and Polygonatum sibiricum powder described in the following examples are commercially available, wherein the content of proanthocyanidins in the mulberry powder and Aronia nigra fruit powder is 10%, and the content of Polygonatum sibiricum polysaccharide in the Polygonatum sibiricum powder is 15%;

The bovine bone collagen peptide and hydrolyzed collagen peptide described in the following examples are commercially available and are both food raw materials with a protein content of 95%;

The vitamin A described in the following examples is commercially available and is a food-grade additive with an effective substance content of 325,000 IU/g;

The vitamin C described in the following examples is commercially available and is a food grade additive with an effective substance content of 97%;

The taurine and ferrous fumarate described in the following examples are commercially available, both are food grade additives, and the effective substance content is 99%;

The vitamin E described in the following examples is commercially available and is a food grade additive with an active ingredient content of 50%;

The yeast powder described in the following examples is commercially available, and has an effective substance niacin content of 40 mg/g and a biotin content of 300 ppm.

In the following embodiments, the term "mixing in equal amounts in increments" means that the equal amounts are taken as the least amount of raw material and the same amount of other raw materials, mixed evenly, and then the same amount of other raw materials are added and mixed evenly, and the amount is increased in multiples until all the raw materials are mixed.

Example 1

(1) Weigh each raw material according to the weight portion recorded in 1# in Table 1, and pass it through a 40-mesh sieve for later use;

(2) mixing the nutritional enhancer and flavoring agent weighed in step (1) in equal amounts in increasing amounts to obtain a mixed raw material A;

(3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, and then fluidized bed drying, granulating with a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 200 rpm, the alcohol dosage is 280 parts of 70% alcohol sprayed within 2 minutes, the fluidized bed drying temperature is 51° C., and the time is 25 minutes;

(4) Mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3), and passing through a 40-mesh sieve to obtain a composition having the function of hair growth and fixation.

Example 2

(1) Weigh the raw materials according to the weight parts recorded in 2# in Table 1, and pass them through a 100-mesh sieve for later use;

(2) mixing the nutritional enhancer and flavoring agent weighed in step (1) in equal amounts in increasing amounts to obtain a mixed raw material A;

(3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, and then fluidized bed drying, granulating with a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 500 rpm, the alcohol dosage is 480 parts of 95% alcohol sprayed within 10 minutes, the fluidized bed drying temperature is 58° C., and the drying time is 35 minutes;

(4) Mixing the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3), and filtering through a 100-mesh sieve to obtain a composition having the function of hair growth and fixation.

Example 3

(1) Weigh the raw materials according to the weight of 3# in Table 1, and pass them through a 60-mesh sieve for later use;

(2) mixing the nutritional enhancer and flavoring agent weighed in step (1) in equal amounts in increasing amounts to obtain a mixed raw material A;

(3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, and then fluidized bed drying, granulating with a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 350 rpm, the alcohol dosage is 300 parts of 85% alcohol sprayed within 5.5 minutes, the fluidized bed drying temperature is 55° C., and the time is 30 minutes;

(4) Mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3), and passing through a 60-mesh sieve to obtain a composition having the function of hair growth and fixation.

Table 1 Amount of each raw material

Comparative Example 1

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 4# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 2

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 5# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 3

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 6# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 4

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 7# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 5

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 8# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 6

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 9# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 7

The same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 10# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 8

The same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 11# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 9

Same as Example 3, except that in step (1), each raw material is weighed according to the weight portion recorded in 12# in Table 2, and passed through a 60-mesh sieve for later use.

Comparative Example 10

Same as Example 3, except that black bean powder is used to replace mulberry powder in step (1).

Table 2 Amount of each raw material

Effect verification

1. Verification of prescription effect

1. Selection of subjects

Select eligible subjects according to the inclusion and exclusion criteria;

1. Selection criteria

(1) 25-45 years old, healthy men or women;

Hair length is between 5 and 40 cm;

(2) Those who have problems with excessive hair loss and mild hair thinning, and the hair count after 60 combings is greater than 10 and still greater than 10 after a 2-week preparation period;

(3) Those who have not undergone any special hair treatments such as hair dyeing, perming, styling, etc. in the past month;

2. Exclusion criteria

(1) Pregnant or breastfeeding women, or those who are planning to get pregnant in the near future;

(2) Those who suffer from mental or psychological diseases; or those who have long-term sleep or emotion control disorders;

(3) Those who have used cosmetics with anti-hair loss effects or other products with such effects or hair growth effects in the past three months;

(4) Those who have taken or topically applied any medication that affects hair growth in the past 6 months;

3. Subject Restrictions

(1) Subjects should not wash their hair within 48±4 hours before screening and each visit during the trial period, and the time before each visit should be basically the same. Subjects should not comb their hair on their own on the day of the visit.

(2) No haircut within 2 weeks before each visit assessment during the trial;

(3) During the trial period, patients are not allowed to take any hair care or hairdressing treatments, nor receive any hair loss prevention or hair growth treatments;

(4) During the trial, you need to maintain your original lifestyle and avoid emotional fluctuations;

(II) Test substances

Test samples: compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 9;

Usage: Test samples are randomly distributed to subjects, and they are instructed on the use of samples to ensure that they use the test substances correctly during the test period. The test substances are used for 12 weeks. During the test, subjects are required to record the use time and any discomfort and adverse reaction symptoms during use;

(III) Test equipment

Comb: The tooth density is moderate (tooth spacing 0.9-1.1mm), the tooth length is 2.0-3.0cm, and the comb length is not less than 10cm (excluding the comb handle). The comb of the same specification and material must be used throughout the test. After each use, it must be disinfected in accordance with the relevant requirements of the Technical Specifications for Disinfection of Medical Institutions (WS-T 367-2012);

(IV) Test process

Subjects were recruited as required, and the number of hair loss was counted and recorded using the 60-combing method;

Qualified subjects underwent a 2-week preparation period, during which 0.9% sodium chloride solution was used. After the preparation period, the subjects were combed 60 times again. Those with hair loss counts still greater than 10 would enter the formal trial.

The subjects selected for the formal trial were divided into 12 groups according to the stratified random method to ensure the balance of factors that may affect the trial results, such as gender, age, hair length, and severity of hair loss;

The selected subjects were evaluated for hair basal values before using the product, including hair loss counts, and recorded; the same evaluation and testing were performed again 4, 8, and 12 weeks after using the product;

(V) Hair loss count: During each visit, trained staff combed the subject's hair 60 times, counted the number of lost hair, and recorded the number.

(VI) Test results

The results of prescription effect verification are shown in Table 3;

Table 3 Prescription effect verification results

VII. Conclusion

According to the data in Table 3, it can be found that the difference in hair loss counts after 12 weeks of Examples 1 to 3 (hair loss counts before product use - hair loss counts after 12 weeks of product use) is higher than that of Comparative Examples 1 to 10. Therefore, the anti-hair loss efficacy of Examples 1 to 3 is better than that of Comparative Examples 1 to 10; in addition, among Examples 1 to 3, the anti-hair loss effect of Example 3 is better than that of Examples 1 and 2.

2. Dose effect verification

1. Experimental Materials and Methods

1. Grouping of mice:

Fifty C57BL/6J mice (SPF grade) aged 38-40 days and weighing 15-20 g (provided by the Experimental Animal Center of Southern Medical University) were randomly divided into five groups of 10 mice each after three days of adaptive feeding. The hair on the back of the mice was shaved 24 hours before administration to induce the hair to enter the growth phase from the resting phase.

2. Sample configuration:

1.54 g of the composition prepared in Example 3 was completely dissolved in 20 mL of water to obtain sample 3#;

0.77 g of the composition prepared in Example 3 was completely dissolved in 20 mL of water to obtain a low-dose sample;

3.08 g of the composition prepared in Example 3 was completely dissolved in 20 mL of water to obtain a high-dose sample;

The finasteride solid is prepared into a 0.2% finasteride solution to obtain a positive control sample;

Sodium chloride solid is prepared into a 0.9% sodium chloride solution to obtain a negative control sample;

3. Drug administration

Sample 3# and four groups of control samples were administered to five groups of mice, respectively, by oral administration once a day, 0.2 mL each time, for 8 weeks.

(II) Experimental results

1. Mouse Weight Changes

During the test, the mice were weighed and recorded every 7 days, and the results were expressed as mean ± standard deviation. The specific results are shown in Table 4;

Table 4 Changes in mouse body weight (unit: g)

It can be found from the data in the above table that during the entire testing process, the weight change trends of the five groups of mice were basically the same, and there were no abnormalities in the mice.

2. Time required for mouse skin changes and hair growth

The skin and hair growth of the depilatory area of each group of mice were observed every day. The specific hair growth conditions are shown in Figures 1 to 5 (photographed every 7 days), and the time when the skin color of the depilatory area of each mouse changed from pink to black and the time when hair began to grow were recorded, expressed as mean ± standard deviation. The specific results are shown in Table 5;

FIG1 is a diagram showing the changes in the back hair of mice treated with sample 3#;

FIG2 is a diagram showing the changes in the back hair of mice administered with a low-dose sample;

FIG3 is a diagram showing the changes in the back hair of mice administered with a high-dose sample;

FIG4 is a diagram showing the changes in the back hair of mice administered with a positive control sample;

FIG5 is a diagram showing the changes in the back hair of mice administered with a negative control sample;

As shown in the figure above, in sample 3#, the skin of 2 mice turned gray at D14, and 2 mice had grown hair. At D21, 4 mice were found to have obvious hair growth, of which 1 mouse had hair growing on 60% of the back area, and 3 mice had hair growing on the entire back area.

In the low-dose sample, the skin of one mouse turned gray on D14, and hair grew in five mice on D21, including two mice with hair growing on 70% of their backs and two mice with hair growing on 80% of their backs.

In the high-dose sample, 5 mice had hair growing on their backs at D14, and 5 mice had hair growing on D21, of which 3 mice had hair growing on 90% of their backs, and 2 mice had hair growing on the entire backs.

In the positive control sample, the skin of 4 mice turned gray at D14, and one mouse had obvious hair growth. At D21, 90% of the back area of 6 mice was found to have hair growth.

In the negative control sample, the skin of two mice turned gray on D14, and hair grew on 70% of the back area of three mice and on 90% of the back area of two mice on D21.

Table 5 Skin changes and time required for hair growth in mice (unit: d)

From the data in the above table, it can be found that compared with the negative control sample, sample 3#, high-dose sample and positive control sample shortened the time to enter the growth phase and grow hair to varying degrees; among them, sample 3# more significantly shortened the time for hair follicles to enter the growth phase and grow hair from the resting phase.

3 Histological examination of mouse back skin

On the 7th, 14th, 28th and 56th days, the mice were humanely killed 24 hours after administration. Two mice were killed each time. Skin specimens were taken from the same part of the shaved area, fixed, routinely prepared, and HE stained. The number of newly formed hair follicles under the microscope was expressed as the mean value. The specific results are shown in Tables 6 to 9 and Figure 6;

Table 6 Histological indexes of mouse skin at 7 days (unit: piece)

Table 7 Histological indexes of mouse skin at day 14 (unit: piece)

Table 8 Histological indexes of mouse skin at 28 days (unit: piece)

Table 9 Histological indexes of mouse skin at day 56 (unit: piece)

HE staining images of sample 3#, low-dose sample, high-dose sample, positive control sample and negative control sample are shown in Figures 7 to 11:

Figure 7 is a HE staining image of sample 3#, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

FIG8 is a HE staining image of a low-dose sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

FIG9 is a HE staining image of a high-dose sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

Figure 10 is a HE staining image of the positive control sample, wherein (a) is a 7-day mouse skin histological HE staining image, (b) is a 14-day mouse skin histological HE staining image, (c) is a 28-day mouse skin histological HE staining image, and (d) is a 56-day mouse skin histological HE staining image;

Figure 11 is the HE staining image of the negative control sample, wherein (a) is the HE staining image of the mouse skin histology at 7 days, (b) is the HE staining image of the mouse skin histology at 14 days, (c) is the HE staining image of the mouse skin histology at 28 days, and (d) is the HE staining image of the mouse skin histology at 56 days.

From Figures 6 to 11, we can see that

Histological examination of the back skin of mice revealed that at D7, D14, and D28, the number of new hair follicles in the negative control group showed a downward trend throughout the entire experimental period, and reached a peak on D7; while the number of new hair follicles in the 3# and high-dose groups reached a maximum on D14, and was zero on D28; the number of new hair follicles in the positive control group reached a peak on D7, and showed a downward trend throughout the entire experimental process.

The number of new hair follicles in the negative control group was lower than that in the 3# and positive control groups at D7 and D14, and the number of new hair follicles in the negative control group was 0 at D28;

The number of new hair follicles in the low-dose group was basically the same as that in the negative control group at D7 and D14, and the number of new hair follicles in the low-dose group was higher than that in the 3# and negative control groups at D28;

The number of new hair follicles in 3# on D7, D14 and D28 was significantly higher than that in the negative control group. The number of new hair follicles on D7 was 48.40% higher than that in the negative control group, and the number of new hair follicles on D14 reached a peak, which was 218.00% higher than that in the negative control group.

The number of new hair follicles in the high-dose group was higher than that in the negative control group at D7 and D14. The number of new hair follicles on D7 was 19.36% higher than that in the negative control group, and the number of new hair follicles on D14 was 32.15% higher than that in the negative control group. The number of new hair follicles on D28 was both 0 and that in the negative control group.

The peak value was reached in the positive control group on D7, and it showed a downward trend after D14. The number of new hair follicles on D28 and the number of new hair follicles in the negative control group were both 0.

In summary, the composition of the present invention has no toxic side effects, and can promote the metabolism and proliferation of hair follicle cells, promote the regeneration of hair follicles, shorten the time for hair follicles to enter the growth phase from the resting phase, has a significant promoting effect on hair growth, and helps to strengthen the firmness of hair in the scalp, thereby achieving the purpose of preventing hair loss and strengthening hair.

The above description is only a preferred specific implementation manner of the present invention, and the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, and they should be covered by the protection scope of the present invention.

Claims (10)

1. A composition having the function of promoting hair growth and fixing hair, characterized in that the raw materials of the composition having the function of promoting hair growth and fixing hair include, by weight:

2. The composition with hair growth and fixation function according to claim 1, characterized in that the protein comprises small molecule peptides and amino acids; the small molecule peptides comprise 500 to 1500 parts of bovine collagen peptides and 500 to 1500 parts of hydrolyzed collagen peptides by weight; and the amino acids comprise 20 to 50 parts of taurine by weight. 3. The composition with hair growth and fixation function according to claim 1, characterized in that the nutritional enhancer is a vitamin enhancer and a mineral enhancer. 4. The composition with hair growth and fixation function according to claim 3, characterized in that the vitamin strengthener comprises, by weight, 0.2 to 10 parts of vitamin A, 10 to 50 parts of vitamin C, 1 to 10 parts of vitamin E and 10 to 50 parts of yeast powder; the mineral strengthener comprises, by weight, 1 to 10 parts of ferrous fumarate and 0.2 to 10 parts of zinc oxide. 5. The composition with hair growth and fixation function according to claim 1, characterized in that the powdered phytosterol ester is β-sitosterol ester. 6. The composition with hair growth and fixation function according to claim 1, characterized in that the millet extract is prepared by: crushing millet, extracting with water, concentrating the extract, and freeze-drying to obtain the millet extract. 7. The composition with hair growth and fixation function according to claim 1, characterized in that the flavoring agent comprises 500-1200 parts of erythritol and 300-600 parts of grapefruit powder by weight. 8. A method for preparing the composition having the function of hair growth and fixation according to any one of claims 1 to 7, characterized in that it comprises the following steps: (1) Weigh each raw material by weight, sieve and set aside; (2) mixing the nutritional enhancer and flavoring agent weighed in step (1) to obtain a mixed raw material A; (3) placing the protein and functional ingredients weighed in step (1) in a granulator and stirring, granulating with alcohol as a binder, and then fluidized bed drying and granulating with a granulator to obtain a mixed raw material B; (4) Mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3), and sieving them to obtain the composition having the function of hair growth and fixation. 9. The preparation method according to claim 8 is characterized in that, in step (1), the sieving is through a 40-100 mesh sieve; in step (2), the mixing is equal and incremental mixing; in step (3), the stirring speed is 200-500 rpm; the alcohol dosage is 280-480 parts by mass of 70-95% alcohol sprayed within 2-10 minutes; the fluidized bed drying temperature is 51-58°C, and the time is 25-35 minutes; the granulator is a 20-mesh granulator; in step (4), the sieving is through a 40-100 mesh sieve. 10. Use of the composition having hair growth and fixation function according to any one of claims 1 to 7 in hair growth and fixation.

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