CN115381925A - A kind of cervical repair dressing and preparation method thereof - Google Patents

Abstract

The invention provides a cervix repairing dressing, which is prepared from aesculus hippocastanum extract, chlorella extract and plant stem cells. The cervical repairing dressing is applied to the treatment of cervical diseases such as cervical erosion, cervical polyp, cervical hypertrophy, cervical cyst, cervical intraepithelial neoplasia, cervical cancer and cervical sarcoma. The cervical repairing dressing in the technical scheme can penetrate into a muscular layer, purify and help to metabolize toxins of cervical tissues, eliminate congestion of the cervical tissues, effectively eliminate discomfort such as itching, red swelling and the like caused by inflammation, play a role in inhibiting the release of cell histamine so as to reduce the pain of a patient, accelerate the repair and regeneration of damaged tissues and capillaries, promote the quick healing of the damaged tissues and promote the healing of wounds. The cervical repairing dressing is a pure natural formula, is natural and non-irritant, adopts a propeller administration mode, has no side effect, can not cause drug resistance of a human body, and has a better healing effect on cervical diseases.

Description

A kind of cervical repair dressing and preparation method thereof

technical field

The invention belongs to the technical field of biomedicine, and in particular relates to a cervical repair dressing and a preparation method thereof.

Background technique

The cervix, also known as the cervix, is one of the important tissues and organs in the female reproductive system. Women's natural aging, improper sex life, improper cleaning and nursing, pregnancy and childbirth contraception and other reasons will cause cervical lesions. Because the cervix is located deep in the vagina, it is difficult for patients to detect cervical lesions when they are mild. After examination, it is inconvenient to treat or take medicine because of the deep location. Cervical lesions are more serious. When symptoms such as pain or bleeding occur, oral medicine, drug application or laser, microwave and other means are often used for treatment. However, laser, microwave and other means of treatment will cause cumulative damage to the nerves and connective tissue around the cervix. Oral medicine may also damage the gastric mucosa, and oral medicine can only provide short-term treatment, and it is easy to relapse. Drug therapy or laser, microwave and other means of treatment can not play a role in the repair and regeneration of cervical tissue, to achieve a long-term healing effect.

Existing coating medicines for the treatment of the cervix contain too many chemical components, which are likely to bring greater irritation to the vagina and cervix, causing discomfort to patients, and may even cause allergies to the vagina and cervix. Chinese patent CN202110967993.X discloses a liquid dressing and its application that has hemostatic function after cervical surgery and prevents infection. Chinese patent CN200810039461.4 discloses a gelatin/bioactive glass composite sponge dressing and its preparation method, all of which use bioactive Glass promotes tissue repair but is prone to stone accumulation. The above-mentioned problems cannot be completely solved.

Contents of the invention

In order to solve the above technical problems, the first aspect of the present invention provides a cervical repair dressing, the raw materials of which include horse chestnut extract, chlorella extract and plant stem cells.

As a preferred technical solution, in parts by weight, the preparation raw materials include 0.1-40 parts of horse chestnut extract, 0.1-30 parts of chlorella extract and 0.1-60 parts of plant stem cells.

Horse chestnut extract can improve the blood circulation of damaged mucosal tissue, accelerate the discharge of metabolic aging tissue and garbage in the vagina, promote the production of actin and myosin, increase the number of fibroblasts, and make the mucosa and muscle layer Tight and elastic, improve the contractility of cervical tissue and then adjust and strengthen its overall shape.

Chlorella extract can penetrate deep into the muscle layer, purify and help metabolize toxins in cervical tissue, remove free radicals in cells, enhance cell vitality, delay cell aging, accelerate the repair of damaged cells and DNA and the regeneration of damaged mucosal tissues, It can better enhance the immune function of the body, form a body repair barrier, expel fluids and toxins in the body in time, and eliminate itching, pain, redness and swelling caused by inflammation.

As a preferred technical solution, the preparation raw materials also include sodium guaiazulene sulfonate.

The inventors found that adding sodium guaiazulene sulfonate to the cervical repair dressing can improve the repair and regeneration and anti-inflammatory effects of the cervical repair dressing on damaged tissues, promote the rapid healing of damaged tissues, and reduce the pain of patients. The inventors believe that the possible reason is that sodium guaiazulene sulfonate can inhibit the migration of leukocytes, thereby improving the anti-inflammatory effect, inhibiting the permeability of capillaries in the cervix, and promoting granulation. Regenerate, repair damaged tissue, thereby promoting wound healing, and inhibit the release of cellular histamine to reduce the patient's pain. However, the addition of sodium guaiazulene sulfonate will increase the roughness of the cervical repair dressing and reduce the comfort of the patient when administering the drug.

As a preferred technical solution, the preparation raw materials also include peptide compounds.

As a preferred technical solution, the peptide compound is an arginine/lysine polypeptide.

The inventors found that the arginine/lysine polypeptide can improve the gelation of the overall cervical repair dressing, significantly improve the integrity of the cervical repair dressing, can fully cover the damaged part of the cervix, and can promote the repair of the cervical repair dressing on the damaged part. Absorption of extravasation and excretion of metabolic waste. Arginine/lysine polypeptide can also better improve the lubricity, water holding capacity and comfort of administration of cervical repair dressings, reduce the roughness of cervical repair dressings, and reduce the excessive roughness of cervical repair dressings during medication. , so that the cervical repair dressing will have greater friction with the vagina and cervix, causing the patient's vaginal pain to increase.

As a preferred technical solution, the plant stem cells are sea fennel stem cells and/or sunflower stem cells.

As a preferred technical solution, the plant stem cells are a mixture of sea fennel stem cells and sunflower stem cells.

As a preferred technical solution, the plant stem cells are a mixture of sea fennel stem cells and sunflower stem cells, and the weight ratio between sea fennel stem cells and sunflower stem cells is (1-10):1.

As a preferred technical solution, the plant stem cells are a mixture of sea fennel stem cells and sunflower stem cells, and the weight ratio between sea fennel stem cells and sunflower stem cells is (5-7):1.

Sea fennel stem cells strengthen the cervical tissue, form a protective film on the surface of the damaged mucosal tissue, reduce the contact between the damaged mucosal tissue, bacteria and metabolites, allow the penetration of gas, avoid re-infection, and promote the discharge and elimination of metabolites Congestion of cervical tissue improves the repair environment of damaged mucosal tissue. By reducing the release of histamine, it effectively relieves the pain caused by inflammation and stimulates allergic reactions, prevents tissue damage, lesions and aging caused by repeated inflammation, and can also clear Vaginal odor. However, the inventor found that the penetration effect of sea fennel stem cells in tissues was poor, and the repair dressing that was pushed into the vagina could not fully exert its effect. Penetration, and can promote the absorption of cells to cervical repair dressings, repair elastic fibers, enhance the tension and contraction of the inner and outer genitals, improve the ability to resist the invasion of germs, and improve the antibacterial and anti-inflammatory properties of the overall drug.

The inventor believes that the internal mechanism of the cervical repair dressing is that when the cervical repair dressing acts on the cervical lesion, it will increase the cell energy ATP, increase the keratinocyte growth factor, increase the production capacity of actin and myosin, and damage the Improve the regeneration ability of cells and mucosal tissue, promote the formation of granulation and epithelial tissue, improve the toughness and contractility of cervical tissue, accelerate the microcirculation of mucosal tissue and the reconstruction of capillaries, thereby reducing the hyperemia of cervical tissue; make pathogenic microorganisms Cell autolysis and death, while reducing the contact time between damaged mucosal tissue and germs and the possibility of cervical tissue lesions, the generation of IL-8&TNF-α and the gene expression of IL-2&IL-6 in cells are reduced, and the relationship between mast cells and Th2 cells The activation ability is enhanced, the activity of COX-2 enzyme, the activation of B cells and T cells, and the activity of NF-κB are reduced, thereby reducing the release of cellular histamine, reducing the chronic inflammation and allergic reactions of the cervix, improving the immune regulation, and eradicating Symptoms of cervical hypertrophy, improve the phagocytic activity of macrophages, enhance the DNA damage repair ability of fibroblasts, promote the blood circulation of damaged mucosal tissues, thereby improving the toxin metabolism ability of cervical tissues, and discharge the mucus retained in the deep gland.

The second aspect of the present invention provides the preparation method of the cervical repair dressing. The preparation method of the cervical repair dressing is as follows: mixing the raw materials uniformly at 20-80° C., and filtering out the material to obtain the product.

The third aspect of the present invention provides that the cervical repair dressing is used in the treatment of cervical erosion, cervical polyps, cervical hypertrophy, cervical cysts, cervical intraepithelial neoplasia, cervical cancer and cervical sarcoma.

The fourth aspect of the present invention provides the method of using the cervical repair dressing in the treatment of cervical diseases: distributing the cervical repair dressing into a gynecological pusher, opening the protective cover of the pusher during use, and inserting the pusher The push tube goes deep into the vagina, and when the push tube reaches the cervix, push the push rod to extrude the cervical repair dressing to cover the cervix.

In addition, all raw materials used are commercially available unless otherwise stated.

Beneficial effect:

(1) The cervical repair dressing in this technical solution can penetrate deep into the muscle layer, purify and help metabolize toxins in cervical tissue, eliminate congestion of cervical tissue, effectively eliminate itching, redness and swelling caused by inflammation, and inhibit the release of cellular histamine. It plays an inhibitory role to reduce the pain of patients, accelerate the repair and regeneration of damaged tissues and capillaries, and promote the rapid healing of damaged tissues, thereby promoting wound healing.

(2) The cervical repair dressing in the technical solution can improve the repair environment of damaged mucosal tissue, prevent tissue damage, pathological changes and aging caused by repeated inflammation, and can also remove vaginal odor.

(3) The cervical repair dressing in this technical solution can repair elastic fibers, promote the formation of collagen, make the skin tighter, improve the defense ability of the body, tighten the inner and outer genitals, enhance the ability to resist the invasion of germs, and improve tension and contraction Force, reshape the body structure.

(4) The cervical repair dressing in this technical solution is a pure natural formula, which is naturally non-irritating and has a high degree of comfort in administration. It adopts the mode of propeller administration without any side effects and will not cause the body to develop drug resistance. It has a good curative effect on cervical diseases.

Description of drawings

Fig. 1. itching sensation test result figure among the present invention;

Fig. 2. wound healing test effect diagram of the present invention;

Fig. 3. skin defense ability test rendering of the present invention;

Fig. 4. The present invention inhibits the expression result of interleukin IL1-β caused by endotoxin;

Fig. 5. The present invention inhibits the expression result of chemokine CXCL10 caused by endotoxin;

Fig. 6. The detection result of the safety index of the present invention.

Detailed ways

Example

Explanation: The specific designations of A to G in the following examples are as follows.

A: sea fennel stem cells, the sea fennel (crithmum maritimum) stem cells were purchased from Chaoniu International Group in Hong Kong, China;

B: sunflower stem cells, the sunflower (Helianthus annuus L.) stem cells were purchased from Zhongnan Yuanpin Stem Cell Technology Park;

C: horse chestnut extract, described horse chestnut (Aesculus chinensis Bunge) extract is purchased from Baoji Fangsheng Biological Development Co., Ltd.;

D: Chlorella extract, which was purchased from Xi'an Ruierli Biological Engineering Co., Ltd.;

E: arginine/lysine polypeptide, the arginine/lysine polypeptide was purchased from Saier Ruicheng (Beijing) Life Science Technology Co., Ltd.;

F: sodium guaiazulene sulfonate, the sodium guaiazulene sulfonate was purchased from Wuhan Kabuda Chemical Co., Ltd.;

G: licorice extract, the licorice (Glycyrrhiza uralensis Fisch.) extract was purchased from IDbio.

Table 1. The raw material composition of the cervical repair dressing provided by embodiment 1～embodiment 11

Example A B C D. E. f G 1 25 copies 5 copies 20 copies 15 copies 3 copies 4 parts 0 2 21 copies 3 copies 15 copies 10 copies 2 copies 3 copies 0 3 0 copies 5 copies 20 copies 15 copies 3 copies 4 parts 0 4 25 copies 0 copies 20 copies 15 copies 3 copies 4 parts 0 5 25 copies 5 copies 0 copies 15 copies 3 copies 4 parts 0 6 25 copies 5 copies 20 copies 0 copies 3 copies 4 parts 0 7 25 copies 5 copies 20 copies 15 copies 0 copies 4 parts 0 8 25 copies 5 copies 20 copies 15 copies 3 copies 0 copies 0 9 25 copies 25 copies 20 copies 15 copies 3 copies 4 parts 0 10 25 copies 2.5 servings 20 copies 15 copies 3 copies 4 parts 0 11 0 copies 5 copies 20 copies 15 copies 3 copies 4 parts 25 copies

Performance Testing

performance test one

Irritation test: The cervical dressings prepared in Examples 1 to 11 were applied to animal epidermis to conduct an irritation test, and the non-irritating ones continued to conduct subsequent tests.

The results show that: Examples 1, 2, 5, 7, 8, 9, 10 and 11 can pass the irritation test and can carry out follow-up tests.

Performance test two

Itching sensation elimination test: Select 60 patients with gynecological diseases and divide them into two groups on average, namely the blank group and the test group. The raw materials in Example 1 are prepared into cervical repair dressings for the test group volunteers, and the blank group volunteers use placebo The dressing was used once a day for 3 consecutive days. Each group was independently observed and counted. The patients recorded and scored the itching sensation. After 3 days, the statistical scoring results (0-10 points, the stronger the itching sensation, the higher the score) are shown in the figure 1. Volunteers in the test group had obvious antipruritic effect after using the cervical repair dressing in Example 1 (that is, the personal care gel in Figure 1); the itching sensation of the volunteers in the blank group did not change significantly.

performance test three

Healing effect test: Examples 1, 2, 5, 7, 8, 9, 10 and 11 can pass the irritation test, and further carry out the cervical disease healing effect test, wherein the curing effect of embodiment 1, 2 is the best, and the comfort is high , the healing effect of embodiment 5,7,8,9,10 and 11 is general, and comfort is higher.

Performance Test Four

Wound healing test: the cervical repair dressing prepared in Example 1 was applied to the epidermal wound of the experimental group, and the placebo dressing was applied to the epidermal wound of the animal in the blank control group. It was used twice a day for 9 consecutive days. Statistics on wound healing, as shown in Figure 2, the epidermal wounds of the animals in the experimental group healed obviously, while the epidermal wounds of the animals in the blank control group healed slowly and not obviously.

Performance Test Five

The cervical dressing prepared in Example 1 was tested for skin defense ability, and the results are shown in FIG. 3 . It can be seen that the obtained cervical auxiliary material has a significant ability to improve skin defense.

Performance Test Six

As shown in Figures 4 and 5, the cervical repair dressing in Example 1 can significantly inhibit the overexpression of interleukin IL1-β and chemokine CXCL10 induced by endotoxin.

Wherein, the HPV gel corresponds to the cervical repair dressing obtained in Example 1.

Performance Test Seven

According to the 2015 edition of the "Safety and Technical Standards for Cosmetics", the cervical repair dressing prepared in Example 1 was tested, and the test results are shown in Figure 6.

Performance Test Eight

Three copies of the cervical repair dressing provided in Example 1 were set up in parallel, and placed at 5°C, 25°C, and 40°C for 15 days respectively. After returning to room temperature (20°C), no difference in properties was observed, and the temperature resistance was stable.

Claims (10)

1. A cervical repair dressing, characterized in that its preparation raw materials include horse chestnut extract, chlorella extract and plant stem cells. 2. The cervical repair dressing according to claim 1, characterized in that, in parts by weight, its preparation raw materials include 0.1-40 parts of horse chestnut extract, 0.1-30 parts of chlorella extract and 0.1-60 parts of plant stem cells. 3. The cervical repair dressing according to claim 1, characterized in that, the preparation raw materials also include sodium guaiazulene sulfonate. 4. The cervical repair dressing according to claim 1, characterized in that, said preparation raw materials also include peptide compounds. 5. The cervical repair dressing according to claim 4, wherein the peptide compound is an arginine/lysine polypeptide. 6. The cervical repair dressing according to claim 1 or 2, wherein the plant stem cells are sea fennel stem cells and/or sunflower stem cells. 7. The cervical repair dressing according to claim 6, wherein the plant stem cells are a mixture of sea fennel stem cells and sunflower stem cells. 8. A method for preparing the cervical repair dressing according to any one of claims 1-7, characterized in that the preparation method of the cervical repair dressing is to mix the raw materials uniformly at 20-80°C, and filter out Material, that is. 9. A cervical repair dressing according to claims 1-7 is used in the treatment of cervical erosion, cervical polyps, cervical hypertrophy, cervical cysts, cervical intraepithelial neoplasia, cervical cancer and cervical sarcoma. 10. A method of using the cervical repair dressing according to claims 1-7 in the treatment of cervical diseases is as follows: distributing the cervical repair dressing in a gynecological pusher, opening the pusher protective cover during use, and pushing the pusher The push tube goes deep into the vagina, and when the push tube reaches the cervix, push the push rod to extrude the cervical repair dressing to cover the cervix.

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