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CN115340935A - Efficient continuous tremella cell separation method and device - Google Patents

Efficient continuous tremella cell separation method and device Download PDF

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CN115340935A
CN115340935A CN202210754996.XA CN202210754996A CN115340935A CN 115340935 A CN115340935 A CN 115340935A CN 202210754996 A CN202210754996 A CN 202210754996A CN 115340935 A CN115340935 A CN 115340935A
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陈铭林
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Fujian Baiaoyuan Biotechnology Co ltd
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Abstract

The invention discloses a high-efficiency continuous tremella cell separation method and a device, relates to the field related to tremella cell separation, and aims to solve the problem that the existing tremella cell separation device cannot continuously separate tremella cells in the prior art.

Description

一种高效连续式银耳细胞分离方法及装置A high-efficiency continuous tremella cell separation method and device

技术领域technical field

本发明涉及银耳细胞分离相关领域,具体为一种高效连续式银耳细胞分离方法及装置。The invention relates to the related field of tremella cell separation, in particular to a high-efficiency continuous tremella cell separation method and device.

背景技术Background technique

银耳是一种担子菌门、银耳纲、银耳目、银耳科、银耳属真菌的子实体,其中所含有的活性成分银耳多糖具有特殊的保健功能,而银耳多糖在进行提取时,往往需要对银耳细胞进行分离处理,从而需要使用细胞分离装置来实现对银耳细胞的分离处理。Tremella is a kind of fruiting body of Basidiomycota, Tremella, Tremella, Tremellaceae, and Tremella genus fungi. The active ingredient Tremella polysaccharide contained in it has special health care functions, and Tremella polysaccharide often needs to be extracted. The cells are separated and processed, so a cell separation device is required to realize the separation and processing of Tremella cells.

例如公告号为CN112457967A的中国授权专利(细胞分离装置及细胞分离方法):包括搅拌腔体、连接装置和分离腔体,搅拌腔体通过连接装置与分离腔体相连,连接装置包括连接管和卡设在连接管上的通道阀门,连接管的两端分别伸入搅拌腔体和分离腔体,通道阀门包括与连接管垂直设置的转轴,转轴中部开设有通孔,通孔匹配连接管的管道设置,转轴端部安设有转动柄。For example, the Chinese authorized patent (cell separation device and cell separation method) whose notification number is CN112457967A: includes a stirring chamber, a connecting device and a separating chamber, the stirring chamber is connected to the separating chamber through a connecting device, and the connecting device includes a connecting tube and a card The passage valve on the connecting pipe, the two ends of the connecting pipe extend into the mixing chamber and the separation chamber respectively, the passage valve includes a rotating shaft perpendicular to the connecting pipe, and a through hole is opened in the middle of the rotating shaft, and the through hole matches the pipe of the connecting pipe The end of the rotating shaft is provided with a rotating handle.

上述现有技术在进行使用时虽然具备固液分离彻底与不易生菌等优点,但其在对银耳细胞的单次细胞分离时,需要装置先对细胞进行分裂再进行离心,才可实现对银耳细胞的提取,在对大量银耳细胞进行提取时,往往需要耗费大量的时间来多次进行细胞分离操作,从而极易影响到使用者的使用体验,因此市场急需研制一种高效连续式银耳细胞分离装置来帮助人们解决现有的问题。Although the above-mentioned prior art has the advantages of thorough solid-liquid separation and not easy to grow bacteria when it is used, it needs a device to split the cells first and then centrifuge the tremella cells in a single cell separation, so as to realize the separation of white fungus cells. For the extraction of cells, when extracting a large number of Tremella cells, it often takes a lot of time to perform cell separation operations many times, which can easily affect the user experience. Therefore, the market urgently needs to develop a high-efficiency continuous Tremella cell separation devices to help people solve existing problems.

发明内容Contents of the invention

本发明的目的在于提供一种高效连续式银耳细胞分离装置,以解决上述背景技术中提出的无法解决现有银耳细胞分离装置难以连续的对银耳细胞进行分离的问题。The purpose of the present invention is to provide a high-efficiency continuous Tremella cell separation device to solve the problem that the existing Tremella cell separation device is difficult to continuously separate Tremella cells proposed in the background technology.

为实现上述目的,本发明提供如下技术方案:一种高效连续式银耳细胞分离装置,包括装置外壳,所述装置外壳的内部设置有透气板,所述透气板上设置有多个透气孔,且透气孔设置为圆孔状,所述透气板的上端设置有第二电动滑道,所述第二电动滑道与透气板通过螺栓连接,所述第二电动滑道的内部设置有第二电动滑块,所述第二电动滑块与第二电动滑道设置为电性连接,所述第二电动滑块上设置有连接架,所述连接架与第二电动滑块通过螺栓连接,所述连接架上设置有固定环,所述固定环设置为圆环状,所述固定环与连接架设置为固定连接,所述固定环的下端设置有旋转块,所述旋转块的外侧设置有调节箱,所述调节箱与旋转块设置为转动连接,所述调节箱与固定环上均设置有连接套,所述连接套设置有多个,所述连接套上设置有连接环,所述连接环的数量与连接套的数量设置为一致,所述连接环与连接套通过螺栓连接。In order to achieve the above object, the present invention provides the following technical solutions: a high-efficiency continuous Tremella cell separation device, comprising a device shell, the inside of the device shell is provided with a ventilating plate, and the ventilating plate is provided with a plurality of ventilating holes, and The ventilation hole is set in the shape of a round hole, the upper end of the ventilation plate is provided with a second electric slideway, and the second electric slideway is connected with the ventilation plate by bolts, and the inside of the second electric slideway is provided with a second electric slideway. The slider, the second electric slider is electrically connected to the second electric slideway, the second electric slider is provided with a connecting frame, and the connecting frame is connected to the second electric slider by bolts, so The connecting frame is provided with a fixed ring, the fixed ring is arranged in a ring shape, the fixed ring is fixedly connected with the connecting frame, the lower end of the fixed ring is provided with a rotating block, and the outer side of the rotating block is provided with An adjustment box, the adjustment box and the rotating block are set to be rotationally connected, the adjustment box and the fixed ring are provided with connecting sleeves, and there are multiple connecting sleeves, and the connecting sleeves are provided with connecting rings. The number of connecting rings is set to be consistent with the number of connecting sleeves, and the connecting rings and connecting sleeves are connected by bolts.

优选的,所述连接环上设置有调节杆,所述调节杆设置有多个,所述调节杆上设置有调节槽,所述调节杆与连接环通过螺栓与调节槽连接,所述调节杆上设置有夹板,所述夹板设置为弧板状,所述夹板上设置有软垫片,且软垫片与夹板设置为粘贴连接,所述夹板的内侧设置有第一分离管,所述第一分离管设置有多个,所述第一分离管的上端设置有第二分离管,所述第二分离管的形状结构数量与第一分离管的形状结构数量均设置为一致。Preferably, the connecting ring is provided with an adjusting rod, and there are multiple adjusting rods, and the adjusting rod is provided with an adjusting groove, and the adjusting rod and the connecting ring are connected with the adjusting groove by a bolt, and the adjusting rod A splint is arranged on the splint, the splint is set in the shape of an arc plate, a soft gasket is arranged on the splint, and the soft gasket and the splint are set to be glued and connected, the inner side of the splint is provided with a first separation tube, and the second There are multiple separation pipes, and the upper end of the first separation pipe is provided with a second separation pipe. The number of shapes and structures of the second separation pipes is set to be consistent with the number of shapes and structures of the first separation pipes.

优选的,所述旋转块的下端设置有贴合套,所述贴合套的下端设置有电机转轴,所述电机转轴的下端设置有驱动电机,所述电机转轴上设置有转动盘,所述转动盘上设置有电动推杆。Preferably, the lower end of the rotating block is provided with a fitting sleeve, the lower end of the fitting sleeve is provided with a motor shaft, the lower end of the motor shaft is provided with a driving motor, and a rotating disk is provided on the motor shaft. An electric push rod is arranged on the rotating disk.

优选的,所述装置外壳的上端设置有密封盖,所述透气板的下端设置有送风机。Preferably, the upper end of the housing of the device is provided with a sealing cover, and the lower end of the air-permeable plate is provided with a blower.

优选的,所述密封盖的下端设置有第一电动滑道,所述第一电动滑道设置有多个,多个所述第一电动滑道呈圆周状布置,所述第一电动滑道与装置外壳通过螺栓连接,所述第一电动滑道的内部设置有第一电动滑块,所述第一电动滑块与第一电动滑道设置为电性连接,所述第一电动滑块上设置有第一电动环道,所述第一电动环道与第一电动滑块通过螺栓连接,所述第一电动环道的一侧设置有隔板,所述隔板与第一电动环道通过螺栓连接,所述隔板的一侧设置有第二电动环道,所述第二电动环道与第一电动环道均设置为半圆弧状,所述第二电动环道与隔板通过螺栓连接。Preferably, the lower end of the sealing cover is provided with a first electric slideway, and there are multiple first electric slideways, and the plurality of first electric slideways are arranged in a circular shape, and the first electric slideway It is connected with the housing of the device by bolts, and a first electric slider is arranged inside the first electric slideway, and the first electric slideway is electrically connected with the first electric slideway, and the first electric slideway A first electric ring is arranged on the top, and the first electric ring is connected with the first electric slider by bolts. One side of the first electric ring is provided with a partition, and the partition and the first electric ring The road is connected by bolts, and a second electric ring is arranged on one side of the partition, and the second electric ring and the first electric ring are both set in a semicircular arc shape, and the second electric ring and the partition pass through Bolted.

优选的,所述第一电动环道的内部设置有第一转动块,所述第一转动块与第一电动环道设置为电性连接,所述第二电动环道的内侧设置有第二转动块,所述第二转动块与第二电动环道设置为电性连接,所述第二转动块与第一转动块上均设置有固定套,所述固定套分别与第二转动块和第一转动块通过螺栓连接。Preferably, the inside of the first electric ring is provided with a first rotating block, the first rotating block is electrically connected with the first electric ring, and the inside of the second electric ring is provided with a second Rotating block, the second rotating block is electrically connected to the second electric circuit, the second rotating block and the first rotating block are provided with fixed sleeves, and the fixed sleeves are respectively connected to the second rotating block and the first rotating block. The first rotating block is connected by bolts.

优选的,所述固定套上设置有第一连接杆,所述第一连接杆与固定套通过螺栓连接,所述第一连接杆的下端设置有防护外壳,所述防护外壳与第一连接杆设置为固定连接,所述防护外壳的内部设置有抽吸泵,所述抽吸泵的下端设置有抽吸管,所述抽吸管与抽吸泵设置为固定连接,所述抽吸泵的一侧设置有导液管,所述导液管与抽吸泵设置为固定连接,所述导液管的一侧设置有存液箱,所述存液箱设置有多个,多个所述存液箱呈半圆周状布置,所述存液箱与装置外壳通过螺栓连接,所述存液箱上设置有进液管,所述进液管上设置有电动阀门,所述进液管与存液箱设置为固定连接。Preferably, the fixed sleeve is provided with a first connecting rod, and the first connecting rod is connected with the fixed sleeve by bolts, and the lower end of the first connecting rod is provided with a protective shell, and the protective shell and the first connecting rod Set as a fixed connection, the inside of the protective shell is provided with a suction pump, the lower end of the suction pump is provided with a suction pipe, the suction pipe and the suction pump are fixedly connected, the suction pump A catheter is provided on one side, and the catheter is fixedly connected to the suction pump. A liquid storage tank is provided on one side of the catheter, and multiple storage tanks are provided. The liquid storage tank is arranged in a semi-circumferential shape, and the liquid storage tank is connected with the device shell by bolts. The liquid storage tank is provided with a liquid inlet pipe, and the liquid inlet pipe is provided with an electric valve. The liquid inlet pipe and the The liquid storage tank is provided as a fixed connection.

优选的,所述防护外壳的一侧设置有第二连接杆,所述第二连接杆的下端设置有超声波破碎器。Preferably, a second connecting rod is provided on one side of the protective shell, and an ultrasonic breaker is provided at the lower end of the second connecting rod.

一种高效连续式银耳细胞分离装置的分离方法,包括如下步骤:A separation method of a high-efficiency continuous tremella cell separation device, comprising the steps of:

步骤一:使用者可以通过开启密封盖来将银耳细胞分别放置在第一分离管与第二分离管的内部,通过电性连接的第二电动环道与第二转动块来驱动超声波破碎器进行移动;Step 1: The user can place the tremella cells in the first separation tube and the second separation tube respectively by opening the sealing cover, and drive the ultrasonic breaker through the electrically connected second electric circuit and the second rotating block move;

步骤二:通过超声波破碎器来伸入对应的第一分离管中,通过超声波破碎器来实现对银耳细胞的破碎处理,破碎完成后,通过电性连接的第二电动滑道与第二电动滑块来转动第一分离管,通过转动的第一分离管来实现对破碎后的银耳细胞进行离心,一段时间后,通过电性连接的第一电动环道与第一转动块来驱动抽吸管进行移动,以实现对第一分离管内部的细胞液进行抽取,从而将分层的细胞液抽吸进存液箱的内部;Step 2: Use the ultrasonic breaker to extend into the corresponding first separation tube, and use the ultrasonic breaker to realize the crushing treatment of Tremella cells. block to rotate the first separation tube, through the rotation of the first separation tube to realize the centrifugation of the broken tremella cells, after a period of time, the suction tube is driven by the electrically connected first electric loop and the first rotating block moving to realize the suction of the cell fluid inside the first separation tube, thereby sucking the stratified cell fluid into the interior of the liquid storage tank;

步骤三:而超声波破碎器在破碎完成后,重新移动超声波破碎器来将超声波破碎器伸入对应的第二分离管中,并重复上述步骤二的操作,以实现对第二分离管中的银耳细胞进行分层处理,同时在第二分离管中银耳细胞进行分层操作时,第一分离管中重新添加的银耳细胞则可以在超声波破碎器的作用下进行破碎,以实现对该分离装置的连续分离。Step 3: After the ultrasonic breaker is crushed, re-move the ultrasonic breaker to extend the ultrasonic breaker into the corresponding second separation tube, and repeat the operation of the above step 2 to realize the cleaning of the white fungus in the second separation tube. The cells are stratified, and when the tremella cells are stratified in the second separation tube, the tremella cells added in the first separation tube can be crushed under the action of the ultrasonic breaker to realize the separation of the separation device. continuous separation.

与现有技术相比,本发明的有益效果是:Compared with prior art, the beneficial effect of the present invention is:

1、该发明通过第一分离管、第二分离管、第一电动环道与第二电动环道的设置,使用者在对该银耳细胞分离装置进行使用时,通过超声波破碎器来伸入对应的第一分离管中,通过超声波破碎器来实现对银耳细胞的破碎处理,破碎完成后,通过电性连接的第二电动滑道与第二电动滑块来转动第一分离管,通过转动的第一分离管来实现对破碎后的银耳细胞进行离心,一段时间后,通过电性连接的第一电动环道与第一转动块来驱动抽吸管进行移动,以实现对第一分离管内部的细胞液进行抽取,从而将分层的细胞液抽吸进存液箱的内部,而超声波破碎器在破碎完成后,重新移动超声波破碎器来将超声波破碎器伸入对应的第二分离管中,并重复上述步骤二的操作,以实现对第二分离管中的银耳细胞进行分层处理,同时在第二分离管中银耳细胞进行分层操作时,第一分离管中重新添加的银耳细胞则可以在超声波破碎器的作用下进行破碎,以实现对该分离装置的连续分离,从而可以在一定程度上解决现有银耳细胞分离装置难以连续的对银耳细胞进行分离的问题;1. In this invention, through the setting of the first separation tube, the second separation tube, the first electric loop and the second electric loop, when the user uses the tremella cell separation device, the ultrasonic breaker is used to insert the corresponding In the first separation tube, the tremella cells are crushed by an ultrasonic breaker. After the crushing is completed, the first separation tube is rotated through the electrically connected second electric slideway and the second electric slider. The first separation tube is used to centrifuge the broken tremella cells. After a period of time, the suction tube is driven to move through the electrically connected first electric loop and the first rotating block, so as to realize the inside of the first separation tube. The cell fluid is extracted, so that the stratified cell fluid is sucked into the interior of the liquid storage tank, and after the ultrasonic breaker is crushed, the ultrasonic breaker is re-moved to extend the ultrasonic breaker into the corresponding second separation tube , and repeat the operation of the above-mentioned step 2, to realize the layering of the Tremella cells in the second separation tube, and when the layering operation of the Tremella cells in the second separation tube is performed, the Tremella cells added again in the first separation tube Then it can be crushed under the action of the ultrasonic breaker to realize the continuous separation of the separation device, thereby solving the problem that the existing tremella cell separation device is difficult to continuously separate the tremella cells to a certain extent;

2、该发明通过第一转动块、第二转动块与存液箱的设置,使用者在使用该银耳细胞分离装置的过程中,使用者可以通过驱动第一转动块与第二转动块进行转动来实现对抽吸结构与破碎结构进行快速切换,同时也可以通过多个存液箱的设置,来实现对分离管中分层的溶液进行收集,从而可以在一定程度上提高整个细胞分离装置的工作效率;2. In this invention, through the setting of the first rotating block, the second rotating block and the liquid storage tank, the user can rotate the first rotating block and the second rotating block during the process of using the tremella cell separation device. To realize the rapid switching between the suction structure and the crushing structure, and at the same time, the collection of the stratified solution in the separation tube can be realized through the setting of multiple liquid storage tanks, so that the efficiency of the entire cell separation device can be improved to a certain extent. work efficiency;

3、该发明通过透气板与送风机的设置,使用者在使用该银耳细胞分离装置的过程中,使用者可以通过送风机的设置,来实现对装置外壳的内部进行加热或降温,并通过透气板的设置,来实现对装置外壳内部温度的传递效率,从而可以大幅度的提高整个银耳细胞分离装置的使用寿命,进而可以在一定程度上缓解使用者的使用压力;3. The invention uses the setting of the air-permeable plate and the air blower. During the process of using the tremella cell separation device, the user can heat or cool down the inside of the device shell through the setting of the air-exhaust plate, and through the air-permeable plate setting to achieve the transfer efficiency of the temperature inside the device shell, which can greatly improve the service life of the whole tremella cell separation device, and thus relieve the user's pressure to a certain extent;

4、该发明通过驱动电机、电动推杆、第二电动滑道与第二电动滑块的设置,使用者在使用该银耳细胞分离装置的过程中,使用者可以通过驱动电机来驱动多个第一分离管进行转动,并通过电动推杆的设置,来实现对第一分离管的高度进行调节,同时也可以通过电性连接的第二电动滑道与第二电动滑块来实现对第二分离管的转动离心,进而可以在一定程度上提高整个银耳细胞分离装置的使用适用性;4. In this invention, through the setting of the drive motor, electric push rod, second electric slideway and second electric slider, the user can drive a plurality of first A separation pipe is rotated, and the height of the first separation pipe can be adjusted through the setting of the electric push rod, and the height of the second separation pipe can also be adjusted through the electrically connected second electric slideway and the second electric slider. The rotation and centrifugation of the separation tube can improve the applicability of the whole tremella cell separation device to a certain extent;

5、该发明通过连接环、调节杆与夹板的设置,使用者在使用该银耳细胞分离装置的过程中,使用者可以通过两个夹板来实现对分离管的夹持,同时也可以通过调节调节杆在连接环上的位置,以实现对不同规格的分离管进行夹取,进而可以在一定程度上缓解使用者的使用压力。5. In this invention, through the setting of the connecting ring, the adjusting rod and the splint, the user can use the tremella cell separation device to clamp the separation tube through the two splints, and can also adjust the The position of the rod on the connecting ring can realize the clamping of separation tubes of different specifications, which can relieve the pressure of the user to a certain extent.

附图说明Description of drawings

图1为本发明的透气板的结构示意图;Fig. 1 is a schematic structural view of a ventilating panel of the present invention;

图2为本发明的局部剖视结构示意图;Fig. 2 is the partial sectional structure schematic diagram of the present invention;

图3为本发明的调节箱的局部剖视结构示意图;Fig. 3 is a partial sectional structural schematic diagram of the regulating box of the present invention;

图4为本发明的A处结构示意图;Fig. 4 is a structural schematic diagram of A place of the present invention;

图5为本发明的防护外壳的局部剖视结构示意图。Fig. 5 is a partial sectional structural schematic diagram of the protective shell of the present invention.

图中:1、装置外壳;2、密封盖;3、第一电动滑道;4、第一电动滑块;5、第一电动环道;6、第一转动块;7、固定套;8、第一连接杆;9、防护外壳;10、抽吸泵;11、抽吸管;12、导液管;13、存液箱;14、进液管;15、固定环;16、连接架;17、第二电动滑道;18、第二电动滑块;19、透气板;20、调节箱;21、驱动电机;22、电机转轴;23、转动盘;24、电动推杆;25、贴合套;26、旋转块;27、连接套;28、连接环;29、调节杆;30、夹板;31、第一分离管;32、第二分离管;33、送风机;34、第二电动环道;35、第二转动块;36、第二连接杆;37、超声波破碎器;38、隔板。In the figure: 1. Device shell; 2. Sealing cover; 3. The first electric slideway; 4. The first electric slider; 5. The first electric ring; 6. The first rotating block; 7. Fixed sleeve; 8 , the first connecting rod; 9, the protective shell; 10, the suction pump; 11, the suction pipe; 12, the catheter; 13, the liquid storage tank; 14, the liquid inlet pipe; 15, the fixed ring; ; 17, the second electric slideway; 18, the second electric slider; 19, the breathable plate; 20, the adjustment box; 21, the driving motor; 22, the motor shaft; 23, the rotating disc; 24, the electric push rod; 25, Fitting sleeve; 26, rotating block; 27, connecting sleeve; 28, connecting ring; 29, adjusting rod; 30, splint; 31, first separation pipe; 32, second separation pipe; 33, air blower; 34, second Electric circuit; 35, the second rotating block; 36, the second connecting rod; 37, the ultrasonic breaker; 38, the dividing plate.

具体实施方式Detailed ways

下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

请参阅图1-5,本发明提供的一种实施例:一种高效连续式银耳细胞分离装置,包括装置外壳1,装置外壳1的内部设置有透气板19,透气板19上设置有多个透气孔,且透气孔设置为圆孔状,透气板19的上端设置有第二电动滑道17,第二电动滑道17与透气板19通过螺栓连接,第二电动滑道17的内部设置有第二电动滑块18,第二电动滑块18与第二电动滑道17设置为电性连接,第二电动滑块18上设置有连接架16,连接架16与第二电动滑块18通过螺栓连接,连接架16上设置有固定环15,固定环15设置为圆环状,固定环15与连接架16设置为固定连接,固定环15的下端设置有旋转块26,旋转块26的外侧设置有调节箱20,调节箱20与旋转块26设置为转动连接,调节箱20与固定环15上均设置有连接套27,连接套27设置有多个,连接套27上设置有连接环28,连接环28的数量与连接套27的数量设置为一致,连接环28与连接套27通过螺栓连接。Please refer to Fig. 1-5, a kind of embodiment provided by the present invention: a kind of efficient continuous Tremella cell separation device, comprises device shell 1, and the interior of device shell 1 is provided with ventilating plate 19, is provided with a plurality of Vent hole, and vent hole is set to circular hole shape, and the upper end of breathable plate 19 is provided with the second electric slideway 17, and the second electric slideway 17 is connected with breathable plate 19 by bolt, and the inside of the second electric slideway 17 is provided with The second electric slider 18, the second electric slider 18 is electrically connected with the second electric slideway 17, the second electric slider 18 is provided with a connecting frame 16, and the connecting frame 16 and the second electric sliding block 18 pass through Bolt connection, the connecting frame 16 is provided with a fixed ring 15, the fixed ring 15 is arranged in a ring shape, the fixed ring 15 and the connecting frame 16 are fixedly connected, the lower end of the fixed ring 15 is provided with a rotating block 26, and the outer side of the rotating block 26 An adjustment box 20 is provided, and the adjustment box 20 and the rotating block 26 are arranged to be rotationally connected. Both the adjustment box 20 and the fixed ring 15 are provided with a connecting sleeve 27, and there are multiple connecting sleeves 27, and the connecting sleeve 27 is provided with a connecting ring 28 , the number of connecting rings 28 is set to be consistent with the number of connecting sleeves 27, and the connecting rings 28 and connecting sleeves 27 are connected by bolts.

进一步,连接环28上设置有调节杆29,调节杆29设置有多个,调节杆29上设置有调节槽,调节杆29与连接环28通过螺栓与调节槽连接,调节杆29上设置有夹板30,夹板30设置为弧板状,夹板30上设置有软垫片,且软垫片与夹板30设置为粘贴连接,夹板30的内侧设置有第一分离管31,第一分离管31设置有多个,第一分离管31的上端设置有第二分离管32,第二分离管32的形状结构数量与第一分离管31的形状结构数量均设置为一致。Further, the connecting ring 28 is provided with an adjusting rod 29, and there are multiple adjusting rods 29. The adjusting rod 29 is provided with an adjusting groove, and the adjusting rod 29 and the connecting ring 28 are connected with the adjusting groove by bolts. 30, the splint 30 is set in the shape of an arc plate, the splint 30 is provided with a soft gasket, and the soft gasket and the splint 30 are set to be pasted and connected, the inner side of the splint 30 is provided with a first separation pipe 31, and the first separation pipe 31 is provided with Multiple, the upper end of the first separation pipe 31 is provided with a second separation pipe 32 , the number of shapes and structures of the second separation pipe 32 is set to be consistent with the number of shapes and structures of the first separation pipe 31 .

进一步,旋转块26的下端设置有贴合套25,贴合套25与旋转块26设置为固定连接,贴合套25的下端设置有电机转轴22,电机转轴22与贴合套25设置为转动连接,电机转轴22的下端设置有驱动电机21,驱动电机21与电机转轴22通过联轴器连接,电机转轴22上设置有转动盘23,转动盘23与电机转轴22设置为固定连接,转动盘23上设置有电动推杆24,电动推杆24设置有多个,电动推杆24与转动盘23设置为固定连接,电动推杆24与旋转块26设置为固定连接。Further, the lower end of the rotating block 26 is provided with a fitting sleeve 25, the fitting sleeve 25 and the rotating block 26 are fixedly connected, the lower end of the fitting sleeve 25 is provided with a motor shaft 22, and the motor shaft 22 and the fitting sleeve 25 are arranged to rotate Connect, the lower end of the motor shaft 22 is provided with a drive motor 21, the drive motor 21 is connected with the motor shaft 22 through a coupling, the motor shaft 22 is provided with a rotating disc 23, the rotating disc 23 and the motor shaft 22 are fixedly connected, and the rotating disc 23 is provided with electric push rod 24, and electric push rod 24 is provided with a plurality of, and electric push rod 24 is set to be fixedly connected with rotating disc 23, and electric push rod 24 is set to be fixedly connected with rotating block 26.

进一步,装置外壳1的上端设置有密封盖2,密封盖2与装置外壳1设置为贴合,密封盖2与装置外壳1通过螺栓连接,透气板19的下端设置有送风机33,送风机33设置有多个,送风机33与装置外壳1通过螺栓连接。Further, the upper end of the device housing 1 is provided with a sealing cover 2, the sealing cover 2 and the device housing 1 are arranged to fit together, the sealing cover 2 and the device housing 1 are connected by bolts, the lower end of the air-permeable plate 19 is provided with a blower 33, and the blower 33 is provided with A plurality of air blowers 33 are connected to the device housing 1 by bolts.

进一步,密封盖2的下端设置有第一电动滑道3,第一电动滑道3设置有多个,多个第一电动滑道3呈圆周状布置,第一电动滑道3与装置外壳1通过螺栓连接,第一电动滑道3的内部设置有第一电动滑块4,第一电动滑块4与第一电动滑道3设置为电性连接,第一电动滑块4上设置有第一电动环道5,第一电动环道5与第一电动滑块4通过螺栓连接,第一电动环道5的一侧设置有隔板38,隔板38与第一电动环道5通过螺栓连接,隔板38的一侧设置有第二电动环道34,第二电动环道34与第一电动环道5均设置为半圆弧状,第二电动环道34与隔板38通过螺栓连接。Further, the lower end of the sealing cover 2 is provided with a first electric slideway 3, the first electric slideway 3 is provided with a plurality, and the plurality of first electric slideway 3 is arranged in a circular shape, the first electric slideway 3 and the device casing 1 Connected by bolts, the inside of the first electric slideway 3 is provided with a first electric slide block 4, the first electric slide block 4 is electrically connected with the first electric slideway 3, and the first electric slide block 4 is provided with a second An electric circuit 5, the first electric circuit 5 and the first electric slider 4 are connected by bolts, one side of the first electric circuit 5 is provided with a partition 38, and the partition 38 and the first electric circuit 5 are connected by bolts Connect, one side of the dividing plate 38 is provided with the second electric loop 34, the second electric loop 34 and the first electric loop 5 are all arranged in a semi-arc shape, the second electric loop 34 and the dividing plate 38 are connected by bolts.

进一步,第一电动环道5的内部设置有第一转动块6,第一转动块6与第一电动环道5设置为电性连接,第二电动环道34的内侧设置有第二转动块35,第二转动块35与第二电动环道34设置为电性连接,第二转动块35与第一转动块6上均设置有固定套7,固定套7分别与第二转动块35和第一转动块6通过螺栓连接。Further, the inside of the first electric ring 5 is provided with a first rotating block 6, the first rotating block 6 is electrically connected with the first electric ring 5, and the inner side of the second electric ring 34 is provided with a second rotating block 35, the second rotating block 35 and the second electric circuit 34 are set to be electrically connected, the second rotating block 35 and the first rotating block 6 are provided with fixed sleeves 7, and the fixed sleeves 7 are respectively connected to the second rotating block 35 and the second rotating block 35. The first rotating block 6 is connected by bolts.

进一步,固定套7上设置有第一连接杆8,第一连接杆8与固定套7通过螺栓连接,第一连接杆8的下端设置有防护外壳9,防护外壳9与第一连接杆8设置为固定连接,防护外壳9的内部设置有抽吸泵10,抽吸泵10的下端设置有抽吸管11,抽吸管11与抽吸泵10设置为固定连接,抽吸泵10的一侧设置有导液管12,导液管12与抽吸泵10设置为固定连接,导液管12的一侧设置有存液箱13,存液箱13设置有多个,多个存液箱13呈半圆周状布置,存液箱13与装置外壳1通过螺栓连接,存液箱13上设置有进液管14,进液管14上设置有电动阀门,进液管14与存液箱13设置为固定连接。Further, the fixed sleeve 7 is provided with a first connecting rod 8, the first connecting rod 8 and the fixed sleeve 7 are connected by bolts, the lower end of the first connecting rod 8 is provided with a protective shell 9, and the protective shell 9 and the first connecting rod 8 are arranged For fixed connection, the inside of protective shell 9 is provided with suction pump 10, and the lower end of suction pump 10 is provided with suction pipe 11, and suction pipe 11 and suction pump 10 are arranged as fixed connection, and one side of suction pump 10 A catheter 12 is provided, and the catheter 12 is fixedly connected to the suction pump 10. One side of the catheter 12 is provided with a liquid storage tank 13, and the liquid storage tank 13 is provided with multiple, multiple liquid storage tanks 13 Arranged in a semi-circular shape, the liquid storage tank 13 is connected to the device shell 1 by bolts, the liquid storage tank 13 is provided with a liquid inlet pipe 14, and the liquid inlet pipe 14 is provided with an electric valve, and the liquid inlet pipe 14 and the liquid storage tank 13 are arranged for a fixed connection.

进一步,防护外壳9的一侧设置有第二连接杆36,第二连接杆36与固定套7通过螺栓连接,第二连接杆36的下端设置有超声波破碎器37,超声波破碎器37与第二连接杆36通过螺栓连接。Further, one side of the protective casing 9 is provided with a second connecting rod 36, the second connecting rod 36 is connected with the fixed sleeve 7 by bolts, and the lower end of the second connecting rod 36 is provided with an ultrasonic breaker 37, and the ultrasonic breaker 37 is connected to the second connecting rod 36. The connecting rods 36 are connected by bolts.

一种高效连续式银耳细胞分离装置的分离方法,包括如下步骤:A separation method of a high-efficiency continuous tremella cell separation device, comprising the steps of:

步骤一:使用者可以通过开启密封盖2来将银耳细胞分别放置在第一分离管31与第二分离管32的内部,通过电性连接的第二电动环道34与第二转动块35来驱动超声波破碎器37进行移动;Step 1: The user can place the tremella cells in the first separation tube 31 and the second separation tube 32 by opening the sealing cover 2 respectively, and the second electric circuit 34 and the second rotating block 35 are electrically connected to separate the tremella cells. Drive ultrasonic breaker 37 to move;

步骤二:通过超声波破碎器37来伸入对应的第一分离管31中,通过超声波破碎器37来实现对银耳细胞的破碎处理,破碎完成后,通过电性连接的第二电动滑道17与第二电动滑块18来转动第一分离管31,通过转动的第一分离管31来实现对破碎后的银耳细胞进行离心,一段时间后,通过电性连接的第一电动环道5与第一转动块6来驱动抽吸管11进行移动,以实现对第一分离管31内部的细胞液进行抽取,从而将分层的细胞液抽吸进存液箱13的内部;Step 2: Insert the ultrasonic breaker 37 into the corresponding first separation tube 31, and use the ultrasonic breaker 37 to realize the crushing treatment of Tremella cells. After the crushing is completed, the second electric slideway 17 electrically connected with The second electric slider 18 rotates the first separating tube 31, and the broken tremella cells are centrifuged through the rotating first separating tube 31. After a period of time, the electrically connected first electric loop 5 and the second A rotating block 6 drives the suction tube 11 to move, so as to extract the cell fluid inside the first separation tube 31, thereby sucking the layered cell fluid into the liquid storage tank 13;

步骤三:而超声波破碎器37在破碎完成后,重新移动超声波破碎器37来将超声波破碎器37伸入对应的第二分离管32中,并重复上述步骤二的操作,以实现对第二分离管32中的银耳细胞进行分层处理,同时在第二分离管32中银耳细胞进行分层操作时,第一分离管31中重新添加的银耳细胞则可以在超声波破碎器37的作用下进行破碎,以实现对该分离装置的连续分离。Step 3: After the ultrasonic breaker 37 is crushed, move the ultrasonic breaker 37 again to extend the ultrasonic breaker 37 into the corresponding second separation pipe 32, and repeat the operation of the above step 2 to realize the second separation The tremella cells in the tube 32 are stratified, and when the tremella cells in the second separation tube 32 are stratified, the tremella cells added in the first separation tube 31 can be crushed under the action of the ultrasonic breaker 37 , to achieve continuous separation of the separation device.

工作原理:使用时,使用者可以通过开启密封盖2来将银耳细胞分别放置在第一分离管31与第二分离管32的内部,通过电性连接的第二电动环道34与第二转动块35来驱动超声波破碎器37进行移动,通过超声波破碎器37来伸入对应的第一分离管31中,通过超声波破碎器37来实现对银耳细胞的破碎处理,破碎完成后,通过电性连接的第二电动滑道17与第二电动滑块18来转动第一分离管31,通过转动的第一分离管31来实现对破碎后的银耳细胞进行离心,一段时间后,通过电性连接的第一电动环道5与第一转动块6来驱动抽吸管进行移动,以实现对第一分离管31内部的细胞液进行抽取,从而将分层的细胞液抽吸进存液箱13的内部,而超声波破碎器37在破碎完成后,重新移动超声波破碎器37来将超声波破碎器37伸入对应的第二分离管32中,并重复上述步骤二的操作,以实现对第二分离管32中的银耳细胞进行分层处理,同时在第二分离管32中银耳细胞进行分层操作时,第一分离管31中重新添加的银耳细胞则可以在超声波破碎器37的作用下进行破碎,以实现对该分离装置的连续分离,而使用者在使用该银耳细胞分离装置的过程中,可以通过驱动第一转动块6与第二转动块35进行转动来实现对抽吸结构与破碎结构进行快速切换,同时也可以通过多个存液箱13的设置,来实现对分离管中分层的溶液进行收集,从而可以在一定程度上提高整个细胞分离装置的工作效率,同时也可以通过送风机33的设置,来实现对装置外壳1的内部进行加热或降温,并通过透气板19的设置,来实现对装置外壳1内部温度的传递效率,从而可以大幅度的提高整个银耳细胞分离装置的使用寿命,进而可以在一定程度上缓解使用者的使用压力,再通过驱动电机21来驱动多个第一分离管31进行转动,并通过电动推杆24的设置,来实现对第一分离管31的高度进行调节,同时也可以通过电性连接的第二电动滑道17与第二电动滑块18来实现对第二分离管32的转动离心,进而可以在一定程度上提高整个银耳细胞分离装置的使用适用性,最后也可以通过两个夹板30来实现对分离管的夹持,同时也可以通过调节调节杆29在连接环28上的位置,以实现对不同规格的分离管进行夹取,进而可以在一定程度上缓解使用者的使用压力。Working principle: When in use, the user can place the tremella cells in the first separation tube 31 and the second separation tube 32 by opening the sealing cover 2, and the second electric circuit 34 electrically connected with the second rotation block 35 to drive the ultrasonic breaker 37 to move, to extend into the corresponding first separation tube 31 through the ultrasonic breaker 37, to realize the crushing treatment of Tremella cells by the ultrasonic breaker 37, after the crushing is completed, through the electrical connection The second electric slideway 17 and the second electric slide block 18 are used to rotate the first separation tube 31, and the broken tremella cells are centrifuged through the rotating first separation tube 31. After a period of time, through the electrically connected The first electric circuit 5 and the first rotating block 6 drive the suction tube to move, so as to extract the cell fluid inside the first separation tube 31, thereby sucking the layered cell fluid into the liquid storage tank 13. Inside, after the ultrasonic breaker 37 is broken, move the ultrasonic breaker 37 again to extend the ultrasonic breaker 37 into the corresponding second separation tube 32, and repeat the operation of the above-mentioned step 2, to realize the second separation tube Tremella cells in 32 are stratified, while Tremella cells in the second separation tube 32 are stratified, Tremella cells added in the first separation tube 31 can be crushed under the action of ultrasonic breaker 37, In order to realize the continuous separation of the separation device, the user can realize the suction structure and the crushing structure by driving the first rotating block 6 and the second rotating block 35 to rotate in the process of using the Tremella cell separation device. Fast switching, and at the same time, the collection of the stratified solution in the separation tube can be realized through the setting of multiple liquid storage tanks 13, so that the working efficiency of the entire cell separation device can be improved to a certain extent, and the blower 33 can also be used. The setting of the device shell 1 is realized to heat or cool down the inside of the device shell 1, and the transmission efficiency of the internal temperature of the device shell 1 is realized through the setting of the air-permeable plate 19, so that the service life of the whole tremella cell separation device can be greatly improved , and then can relieve the pressure of the user to a certain extent, and then drive a plurality of first separation pipes 31 to rotate through the drive motor 21, and realize the height adjustment of the first separation pipe 31 through the setting of the electric push rod 24 At the same time, the rotation and centrifugation of the second separation tube 32 can also be realized through the electrically connected second electric slideway 17 and the second electric slide block 18, thereby improving the use of the whole tremella cell separation device to a certain extent. Applicability, finally, the separation tube can be clamped by two splints 30, and the position of the adjustment rod 29 on the connecting ring 28 can also be adjusted to realize the clamping of the separation tube of different specifications, and then can To a certain extent, the user's pressure is relieved.

对于本领域技术人员而言,显然本发明不限于上述示范性实施例的细节,而且在不背离本发明的精神或基本特征的情况下,能够以其他的具体形式实现本发明。因此,无论从哪一点来看,均应将实施例看作是示范性的,而且是非限制性的,本发明的范围由所附权利要求而不是上述说明限定,因此旨在将落在权利要求的等同要件的含义和范围内的所有变化囊括在本发明内。不应将权利要求中的任何附图标记视为限制所涉及的权利要求。It will be apparent to those skilled in the art that the invention is not limited to the details of the above-described exemplary embodiments, but that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Accordingly, the embodiments should be regarded in all points of view as exemplary and not restrictive, the scope of the invention being defined by the appended claims rather than the foregoing description, and it is therefore intended that the scope of the invention be defined by the appended claims rather than by the foregoing description. All changes within the meaning and range of equivalents of the elements are embraced in the present invention. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (9)

1. The utility model provides a high-efficient continuous type white fungus cell separation device, includes device shell (1), its characterized in that: the device is characterized in that a ventilation plate (19) is arranged in the device shell (1), a plurality of ventilation holes are formed in the ventilation plate (19), the ventilation holes are arranged to be circular holes, a second electric slide (17) is arranged at the upper end of the ventilation plate (19), the second electric slide (17) is connected with the ventilation plate (19) through bolts, a second electric slide (18) is arranged in the second electric slide (17), the second electric slide (18) and the second electric slide (17) are electrically connected, a connecting frame (16) is arranged on the second electric slide (18), the connecting frame (16) is connected with the second electric slide (18) through bolts, a fixing ring (15) is arranged on the connecting frame (16), the fixing ring (15) is arranged to be a connecting sleeve, the fixing ring (15) is fixedly connected with the connecting frame (16), a rotating block (26) is arranged at the lower end of the fixing ring (15), an adjusting box (20) is arranged at the outer side of the rotating block (26), the adjusting box (20) is rotatably connected with the rotating block (26), a plurality of connecting sleeves (27) are arranged on the adjusting box (20), and a plurality of fixing rings (27) are arranged on the connecting sleeve (27), the number of the connecting rings (28) is consistent with that of the connecting sleeves (27), and the connecting rings (28) are connected with the connecting sleeves (27) through bolts.
2. The efficient continuous tremella cell separating device as claimed in claim 1, wherein: be provided with on go-between (28) and adjust pole (29), it is provided with a plurality ofly to adjust pole (29), be provided with the adjustment tank on adjusting pole (29), it is connected with the adjustment tank through the bolt with go-between (28) to adjust pole (29), be provided with splint (30) on adjusting pole (29), splint (30) set up to the arc platelike, be provided with the soft gasket on splint (30), and soft gasket and splint (30) set up to paste and are connected, the inboard of splint (30) is provided with first separator tube (31), first separator tube (31) are provided with a plurality ofly, the upper end of first separator tube (31) is provided with second separator tube (32), the shape structure quantity of second separator tube (32) and the shape structure quantity of first separator tube (31) all set up to the unanimity.
3. The efficient continuous tremella cell separating device as claimed in claim 1, wherein: the lower extreme of rotatory piece (26) is provided with laminating cover (25), the lower extreme of laminating cover (25) is provided with motor shaft (22), the lower extreme of motor shaft (22) is provided with driving motor (21), be provided with rolling disc (23) on motor shaft (22), be provided with electric putter (24) on rolling disc (23).
4. The efficient continuous tremella cell separating device as claimed in claim 1, wherein: the upper end of the device shell (1) is provided with a sealing cover (2), and the lower end of the ventilation plate (19) is provided with a blower (33).
5. The efficient continuous tremella cell separating device as claimed in claim 4, wherein: the lower extreme of sealed lid (2) is provided with first electronic slide (3), first electronic slide (3) are provided with a plurality ofly, and are a plurality of first electronic slide (3) are the circumference form and arrange, first electronic slide (3) pass through bolted connection with device shell (1), the inside of first electronic slide (3) is provided with first electronic slider (4), first electronic slider (4) set up to electric connection with first electronic slide (3), be provided with first electronic loop (5) on first electronic slider (4), first electronic loop (5) pass through bolted connection with first electronic slider (4), one side of first electronic loop (5) is provided with baffle (38), baffle (38) pass through bolted connection with first electronic loop (5), one side of baffle (38) is provided with second electronic loop (34), second electronic loop (34) all set up to the semicircle arc-shape with first electronic loop (5), second electronic loop (34) pass through bolted connection with electronic loop (38).
6. The efficient continuous tremella cell separating device as claimed in claim 5, wherein: the inside of first electronic ring way (5) is provided with first turning block (6), first turning block (6) set up to electric connection with first electronic ring way (5), the inboard of second electronic ring way (34) is provided with second turning block (35), second turning block (35) set up to electric connection with second electronic ring way (34), all be provided with fixed cover (7) on second turning block (35) and first turning block (6), fixed cover (7) pass through bolted connection with second turning block (35) and first turning block (6) respectively.
7. The efficient continuous tremella cell separating device as claimed in claim 6, wherein: be provided with head rod (8) on fixed cover (7), head rod (8) and fixed cover (7) pass through bolted connection, the lower extreme of head rod (8) is provided with protecting sheathing (9), protecting sheathing (9) set up to fixed connection with head rod (8), the inside of protecting sheathing (9) is provided with suction pump (10), the lower extreme of suction pump (10) is provided with suction tube (11), suction tube (11) set up to fixed connection with suction pump (10), one side of suction pump (10) is provided with catheter (12), catheter (12) set up to fixed connection with suction pump (10), one side of catheter (12) is provided with deposit liquid case (13), deposit liquid case (13) is provided with a plurality ofly, and is a plurality of deposit liquid case (13) are half week form and arrange, deposit liquid case (13) and device shell (1) pass through bolted connection, be provided with feed liquor pipe (14) on deposit liquid case (13), be provided with electric valve on feed liquor pipe (14), deposit liquid case (14) sets up to fixed connection with feed liquor deposit liquid case (13).
8. The efficient continuous tremella cell separating device as claimed in claim 7, wherein: one side of the protective shell (9) is provided with a second connecting rod (36), and the lower end of the second connecting rod (36) is provided with an ultrasonic crusher (37).
9. A separation method of a high-efficiency continuous tremella cell separation device is based on any one of claims 1 to 8, and is characterized by comprising the following steps:
the method comprises the following steps: a user can place tremella cells in the first separation tube (31) and the second separation tube (32) respectively by opening the sealing cover (2), and the ultrasonic crusher (37) is driven to move by the second electric loop (34) and the second rotating block (35) which are electrically connected;
step two: the tremella cells are crushed by the ultrasonic crusher (37) and extend into the corresponding first separating tube (31), the tremella cells are crushed by the ultrasonic crusher (37), after the tremella cells are crushed, the first separating tube (31) is rotated by the electrically connected second electric slide way (17) and the second electric slide block (18), the crushed tremella cells are centrifuged by the rotated first separating tube (31), and after a period of time, the suction tube (11) is driven to move by the electrically connected first electric loop (5) and the first rotating block (6) so as to extract the cell sap in the first separating tube (31) and suck the layered cell sap into the liquid storage box (13);
step three: and after the ultrasonic crusher (37) is crushed, the ultrasonic crusher (37) is moved again to enable the ultrasonic crusher (37) to stretch into the corresponding second separation tube (32), and the operation of the second step is repeated, so that the tremella cells in the second separation tube (32) are subjected to layering treatment, and meanwhile, when the tremella cells in the second separation tube (32) are subjected to layering operation, the tremella cells added again in the first separation tube (31) can be crushed under the action of the ultrasonic crusher (37), so that the separation device can be continuously separated.
CN202210754996.XA 2022-06-30 2022-06-30 Efficient continuous tremella cell separation method and device Pending CN115340935A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020187547A1 (en) * 1998-12-24 2002-12-12 Cepheid Container for holding cells or viruses for disruption
JP2006180756A (en) * 2004-12-27 2006-07-13 Tosho Denki Kk Ultrasonic cell crushing apparatus
CN208943509U (en) * 2018-10-10 2019-06-07 长春职业技术学院 A kind of ultrosonic extractor for tremella polysaccharides
CN215178998U (en) * 2020-11-30 2021-12-14 郑州迪安医学检验所有限公司 Centrifugal machine for food detection and thawing
CN215627910U (en) * 2021-06-17 2022-01-25 北京安溢生物科技有限公司 Stem cell extraction element
CN215947293U (en) * 2021-03-17 2022-03-04 济南科为生物科技有限公司 High-efficient biological tissue broken handle device

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020187547A1 (en) * 1998-12-24 2002-12-12 Cepheid Container for holding cells or viruses for disruption
JP2006180756A (en) * 2004-12-27 2006-07-13 Tosho Denki Kk Ultrasonic cell crushing apparatus
CN208943509U (en) * 2018-10-10 2019-06-07 长春职业技术学院 A kind of ultrosonic extractor for tremella polysaccharides
CN215178998U (en) * 2020-11-30 2021-12-14 郑州迪安医学检验所有限公司 Centrifugal machine for food detection and thawing
CN215947293U (en) * 2021-03-17 2022-03-04 济南科为生物科技有限公司 High-efficient biological tissue broken handle device
CN215627910U (en) * 2021-06-17 2022-01-25 北京安溢生物科技有限公司 Stem cell extraction element

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