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CN115316397A - Application of bactericidal composition for preventing and treating diaporthe citri - Google Patents

Application of bactericidal composition for preventing and treating diaporthe citri Download PDF

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Publication number
CN115316397A
CN115316397A CN202211099457.3A CN202211099457A CN115316397A CN 115316397 A CN115316397 A CN 115316397A CN 202211099457 A CN202211099457 A CN 202211099457A CN 115316397 A CN115316397 A CN 115316397A
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citrus
pyraclostrobin
mancozeb
composition
diaporthe citri
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Inventor
李林虎
李新生
孙敬权
蒋伟
马衍峰
孙丽梅
孔令飞
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Limin Chemical Co ltd
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Limin Chemical Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A01N47/14—Di-thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses an application of a sterilization composition for preventing and treating aschersonia citrosum, wherein the sterilization composition takes mancozeb and pyraclostrobin as main effective components, and the mass ratio of the mancozeb to the pyraclostrobin is 1-16; the application is to prevent or control the main stem, branch, leaf and fruit of citrus from being infected by toxin formed by the citrus inhabitant. The mancozeb and the pyraclostrobin are compounded, so that the bactericide has an obvious treatment effect on gummosis caused by diaporthe citrea infection, and shows an excellent synergistic sterilization effect. Meanwhile, the mancozeb and the pyraclostrobin are compounded, so that the risk that the pyraclostrobin is easy to generate drug resistance in the using process can be reduced.

Description

Application of bactericidal composition for preventing and treating diaporthe citri
Technical Field
The invention belongs to the field of pesticides, and relates to an application of a sterilization composition for preventing and treating Ascomycotina sp.
Background
Mancozeb, english generic name: mancozeb, chemical name: manganese and zinc ethylene bis-dithiocarbamate complex salts. Mancozeb is a protective bactericide with a wide bactericidal spectrum, mainly inhibits the oxidation of pyruvic acid in thalli, is effective on various diseases such as anthracnose, early blight and the like on fruit trees and vegetables, and is often mixed with a systemic bactericide to delay the generation of resistance.
Pyraclostrobin (Pyraclostrobin), also known as pyraclostatin, formula C 19 H 18 ClN 3 O 4 Chemical name of N- [2- [ [1- (4-chlorophenyl) pyrazol-3-yl]Oxymethyl radical]Phenyl radical]-methyl N-methoxycarbamate. Pyraclostrobin is a novel broad-spectrum bactericide, has an action mechanism of a mitochondrial respiration inhibitor, and has the effects of protection, treatment and leaf penetration and conduction. The results of field efficacy tests show that the pyraclostrobin missible oil has good control effects on powdery mildew, downy mildew, banana scab, leaf spot, sclerotinia, and the like of cucumbers, has systemic conductivity and rain wash resistance, and has a wide application range.
Citrus resinifera, also known as sand skin disease and stem rot disease, is a disease which is caused by infection of diaporthe citri and occurs on citrus, mainly harms branches, fruits, leaves and other parts, and can be classified into 4 types in citrus orchard, namely, gummosis type, dry-dry type, sand skin type and stem rot type. When serious, the method causes large-area garden damage, influences the service life of trees or a large amount of rotten fruits in the process of storage and transportation, and influences the commodity value of the fruits.
In order to reduce or prevent the infestation of citrus by the fungus of the genus diaporthe, the measures currently used are mainly the following:
agricultural control: cultivating a variety which is not easily infected by citrus interstation shell fungi, and introducing disease-free seedling diseases; organic fertilizer is additionally applied in autumn and after fruit picking, soil is improved, cold and freezing are prevented, other plant diseases and insect pests are prevented, and wounds are avoided; in early spring, the tree trunk is whitered, reasonably pruned and prevented by a medicament; the trunks are whitered in summer, sunburn is prevented, the pruning is reasonable, and the humidity of the orchard is reduced by paying attention to ventilation and drainage in rainy seasons; cleaning the garden in winter, scraping off gummosis hard blocks and rotten cortex and wood layers at the lower part, thoroughly cutting off resin diseased branches and resin diseased leaves, burning out in a concentrated manner to reduce sources of orchard pathogens, and whitening trunks; applying calcium magnesium phosphate fertilizer additionally, supplementing trace elements and avoiding the lack of element and yellowing of leaves; reasonably pruning, leaving a proper amount of fruits to avoid the overload of fruit trees, harvesting in time, lightly picking and lightly releasing, reducing fruit injury, removing diseased fruits and damaged fruits in time, packaging and storing, and combining some antiseptic agents and measures to prevent and reduce the stem rot of the fruits.
Chemical control: the control result of the bactericide on the acerola citruses shows that the concentration of 16% of imiben-iprodione is 320 mg/l, the concentration of 325 g/l of phenylate-azoxystrobin is 435.5 mg/l, the concentration of 42.4% of azolidone-flutolanil is 211.8 mg/l, the concentration of 430g/l of tebuconazole is 71.6 mg/l, the concentration of 27.8% of thifluzamide-hexaconazole is 184.8 mg/l, and the maximum concentration of 10% of hexaconazole is 70 mg/l, so that the bactericide has a remarkable inhibiting effect on the growth of the acerola citruses.
However, agricultural control is a purely defensive measure, and efficiency, efficacy are not satisfactory and reliable, especially when prevailing climatic conditions are favorable for fungal infestation. Similarly, the effects of the above chemical control agents are reduced with time in practical use, with the development of drug resistance.
Disclosure of Invention
The invention aims to provide the application of the sterilizing composition containing mancozeb and pyraclostrobin in preventing or controlling diaphora citri which is a fungus of ascomycotina in oranges.
The purpose of the invention is realized by the following technical scheme:
the application of the bactericidal composition for preventing and treating the aschersonia citrifolia is characterized in that the bactericidal composition takes mancozeb and pyraclostrobin as main effective components, and the mass ratio of the mancozeb to the pyraclostrobin is 1-16.
The mass ratio of mancozeb to pyraclostrobin is preferably 1 to 14, and more preferably 1 to 9, and can be selected from the following 1.
The citrus chaetomium is a compound species of a whole genome sequence ZJUD2 and ZJUD 14.
Preferably, the application is to prevent or control infection of the trunk, branch, leaf and fruit of citrus by toxins formed by aschersonia aurantiaca of the subdivision ascomycotina.
Preferably, the citrus plant is sugar orange, wogonin, mandarin orange, emperor orange, ponkan orange, oriental tangerine, ugly orange and the like.
Preferably, the application is the treatment of citrus plants with the fungicidal composition or the treatment of soil in which citrus plants grow with the fungicidal composition.
Further preferably, said application is the simultaneous or separate treatment of the trunk, branches, leaves and fruits of citrus with the germicidal composition.
Further preferably, the application is root irrigation of citrus plants by using the bactericidal composition.
The fungicidal compositions of the invention may also be used in combination with other agents having herbicidal, insecticidal or fungicidal properties, especially in combination with a protective fungicide, or with an insecticide, protectant, growth regulator, phytonutrient or soil conditioner.
If mancozeb and pyraclostrobin are used in combination with other fungicides, they are preferably used in combination with one or two other fungicides.
In particular, mancozeb and pyraclostrobin may be applied in combination with the following fungicides:
acylalanines, such as benalaxyl (benalaxyl), metalaxyl (metalaxyl), furoylamide (ofarace), oxadixyl (oxadixyl);
amine derivatives, such as 4-dodecyl-2, 6-dimethylmorpholine (aldimorph), dodine (dodine), dodemorph (dodemorph), fenpropimorph (fenpropimorph), fenpropidin (fenpropidine), biguanide salts (guazatine), iminoctadine acetate (iminoctadine), spiroxamine (spiroxamine), tridemorph (tridemorph);
anilinopyrimidines, such as pyrimethanil, mepanipyrim or cyprodinil;
antibiotics, such as cycloheximide (cycloheximide), griseofulvin (griseofulvin), kasugamycin (kasugamycin), polymalemycin (natamycin), polyoxin (polyoxin) or streptomycin (streptomycin);
azoles, such as bitertanol (bitertanol), bromuconazole (bromoconazole), cyproconazole (cyproconazole), difenoconazole (difenoconazole), diniconazole (dinitroconazol), fenbuconazole (fenbuconazole), fluquinconazole (fluquinconazole), flusilazole (flusilazole), hexaconazole (hexaconazole), imazalil (imazalil), myclobutanil (mycobutyranil), penconazole (penconazole), propiconazole (propiconazole), prochloraz (prochlororaz), prothioconazole (prothioconazole), tebuconazole (tebuconazole), triadimefon (triabendazole), triadimenol (triabendazole), triflumizole (triflumizole), triticonazole (triticonazole), 5-4-methyl-4- (1-4-methyl-phenyl-4-methyl-4-phenyl-4- (1-4-methyl-phenyl-4-methyl-triazole [1,5,6,5,5,4,5,4,2,4,5,;
dicarboximides, such as iprodione, myclozolin, procymidone, vinclozolin;
dithiocarbamates, such as ferbam, sodium (nabam), maneb (maneb), metam (metam), metiram (metiram), propineb (propineb), propineb (polycarbamate), thiram (thiram), ziram (ziram), zineb (zineb);
heterocyclic compounds, such as trichlorfon (anilazine), benomyl (benomyl), boscalid (boscalid), carbendazim (carbendazim), carboxin (carboxin), carboxin (oxacarboxin), cyazofamid (cyazofamid), dazomet (dazomet), dithianon (dithianon), oxazobium (famoxadone), fenamidone (fenamidone), isocrimol (fenamol), fuberidazole (furapizone), flutolanil (flutolanil), furametpyr (furametpyr), mepronil (mepronil), nuarimol (nurimol), thiabendazole (probenazole), proquinazid (proquinazid), praziquantel (pyrifenox), pyroquillofen (pyroquillox), quinoxyfen (silthiofam), thiabendazole (thiabendazole), fludioxonil (thifluzamide), thiophanate-methyl (thiophanate-methyl), tiadinil (tiadinil), tricyclazole (tricyclazole);
copper fungicides, such as bordeaux mixture, copper acetate, copper oxychloride (copperoxochloride), basic copper sulfate;
phenylpyrroles, such as fenpiclonil or fluoroxacillin (fluoroxonil);
sulfenic acid derivatives, such as captafol (captafol), captan (captan), dichlofluanid (dichlofluanid), folpet (folpet), tolylfluanid (tolyfluoride);
cinnamide and similar compounds, such as dimethomorph (dimethomorph), flurbiprofen (flumetover) or flumorph (flumorph);
strobilurins (strobilurins), such as azoxystrobin (azoxystrobin), dimoxystrobin (dimoxystrobin), fluoxastrobin (fluoxastrobin), fluopyram (kresoxim-methyl), metominostrobin (metominostrobin), orysastrobin (orysastrobin), picoxystrobin (picoxystrobin), or trifloxystrobin (trifloxystrobin);
other fungicides are used in combination with other fungicides, such as thiadiazoline (acibenzolar-S-methyl), benthiavalicarb (benthiavalicarb), chlorocyclopropanamide (carpropamid), chlorothalonil (chlorothalonil), cyflufenamid (cyflufenamid), cymoxanil (cymoxanil), diclomezine (diclomezine), diclocyanamide (diclocymet), diethofencarb (diethofencarb), edifenphos (edifenphos), ethaboxam (ethaboxam), fenhexamid (fenhexamid), fenpyrad (fenoxanil), pyrimethanil (ferimzone), fluazinam (fluazinam), fosetyl-aluminium (fosetyl-alum), iprovalicarb, hexachlorobenzene (hexachlorobenzene), fenpropiconazole (fenpropiophenone), penfluazuron (penflufenuron), pencyflufen (tetrachloropenoxsulam), chlorothalonil (chlorothalonil).
The bactericidal composition can be mainly prepared in a preparation form, namely, all the substances in the composition are mixed; the active ingredients of the germicidal composition may also be provided in a single dose, mixed in a tub or tank prior to use, and then diluted to the desired concentration. Preferably, the preparation provided by the invention is mainly used.
Preferably, the sterilization composition is wettable powder, a suspending agent or water dispersible granules prepared by taking mancozeb and pyraclostrobin as main active ingredients.
Further preferably, in the bactericidal composition, the total mass fraction of mancozeb and pyraclostrobin is 40-75%.
The invention has the beneficial effects that:
the traditional chemical control method has the defects that a lot of areas have drug resistance along with the lapse of time, so that the use amount of pesticides is increased year by year. After strict biological test screening, the inventor surprisingly finds that the traditional protective bactericide mancozeb and the pyraclostrobin are compounded, so that the bactericidal composition has an obvious treatment effect on the resinosis caused by the infection of the diaporthe citri, and shows an excellent synergistic bactericidal effect.
Similarly, the traditional agricultural control is time-consuming and labor-consuming, has an unobvious effect, and is easy to treat unclean so as to cause the continuous spread of diseases. The method for treating soil or main stems, branches, leaves and fruits of citrus plants greatly improves the killing effect on the aschersonia citrullus.
Meanwhile, the mancozeb and the pyraclostrobin are compounded, so that the risk that the pyraclostrobin is easy to generate drug resistance in the using process can be reduced.
Detailed Description
The technical solutions of the present invention are explained by using preferred embodiments, and it should be understood that the preferred embodiments described herein are only used for explaining and explaining the present invention, and do not limit the present invention.
"%" is mass fraction.
Example 1
32% mancozeb +8% pyraclostrobin suspending agent
Figure BDA0003839165410000051
After being crushed by a superfine crusher, the pyraclostrobin original drug is evenly mixed with other components, and then the suspending agent is obtained after high-speed shearing.
Example 2
70% mancozeb and 5% pyraclostrobin water dispersible granule
Figure BDA0003839165410000052
Figure BDA0003839165410000061
The components are weighed according to the formula, evenly mixed, crushed by an ultrafine crusher, mixed by adding water, extruded and granulated, dried and screened to obtain the 56 percent mancozeb and 4 percent trifloxystrobin water dispersible granules with the granularity of 20-80 meshes.
Example 3
Wettable powder of 64% mancozeb and 8% pyraclostrobin
Figure BDA0003839165410000062
The components are weighed according to the formula, evenly mixed and crushed by an ultrafine crusher to obtain 64 percent mancozeb and 8 percent pyraclostrobin wettable powder.
And (3) bioassay test:
and (3) measuring indoor toxicity of the pyraclostrobin and the mancozeb to citrus resiniferous pathogens.
The citrus hypocotyl is citrus hypocotyl (Diaporthe citri): the whole genome sequences ZJUD2 and ZJUD14 are in a compound variety.
The test medium was a PDA medium: 200g of potato, 20g of glucose and 20g of agar, and distilled water is added to the mixture to reach 1000mL.
The activity of the single medicament and the compound medicament on the diaporthe citri is measured indoors by a hypha growth rate method. The single preparation and the compound preparation are prepared by DMSO, the medicaments with corresponding concentrations are added into a preheated sterilized PDA culture medium to prepare medicament-containing flat plates with different concentrations, and a blank PDA flat plate without the medicaments is used as a reference. Using a puncher with the diameter of 5mm to select the edge of the citrus intercalary shell colony cultured for 7 days at 28 ℃ in the dark to punch and prepare a fungus cake, then using an inoculating needle to pick the fungus cake, and respectively inoculating the fungus cake on PDA plates with different concentrations, wherein the PDA plates contain mancozeb, pyraclostrobin and a compound medicament. Each plate was placed in a biochemical incubator and incubated at 28 ℃ for 7 days in the dark, and the colony diameter was measured and recorded. Each treatment was repeated 3 times and the experiment was repeated 3 times.
Calculating the growth inhibition rate of the agents with each concentration on pathogenic bacteria according to the linear growth rate of the citrus intercropping fungi on the flat plates with the agents with different concentrations, and calculating the EC of the single agent and the mixed agent with each treatment ratio by using DPS software 50 And calculating the synergy coefficient SR under different proportions according to a Wadley (1945) method, and judging the combined action mode of the mixed medicaments according to the SR value: SR < 0.5 is antagonistic; 1.5 SR is more than or equal to 0.5 for additive effect; SR > 1.5 is synergistic effect.
TABLE 1 indoor bioassay result of pyraclostrobin and mancozeb compounded pair diaporthe citri
Figure BDA0003839165410000071
Analysis and evaluation: the mixed mancozeb and pyraclostrobin has higher bactericidal activity to citrus resiniferous diseases, and the mass ratio of mancozeb to pyraclostrobin is (1)7 50 0.0136, 0.0166, 0.0180, 0.0203, 0.0220, 0.0254, 0.0387, 0.0614 and 0.1094 respectively, and the synergistic coefficients are 0.94, 1.92, 2.13, 2.21, 2.33, 2.27, 1.65, 1.56 and 0.99 respectively. According to the Wadley (1945) method, 1.5 is more than or equal to SR more than or equal to 0.5, and is additive effect, SR more than 1.5 is synergistic effect, the mass ratio of mancozeb to pyraclostrobin is 4; the mass ratio of mancozeb to pyraclostrobin is 1, the synergistic coefficient of 1 is 0.5-1.5, and the addition effect is as follows. However, in actual production, special environment, processing technology and other factors are needed to be further comprehensively considered.
The citrus tristimania (the whole genome sequences ZJUD2 and ZJUD14 composite species) used in the embodiment of the invention is only used for illustrating that the mancozeb and the pyraclostrobin have a synergistic effect on the citrus tristimania after being compounded, but the citrus tristimania used is not limited to the source. It will be clear to those skilled in the art that the mancozeb of the invention has a synergistic effect on pyraclostrobin after being compounded therewith, as long as the compound identified as diaphora citri is used.
Biological control example: field test for preventing and treating citrus resiniferosis
Crop and target: citrus tree (sugar orange, or citrus), resinosis
The experimental conditions are as follows: the method is carried out in Guangxi Guilin orchard from 20 months in 2020 to 25 months in 2020.
Medicament: example 1-example 3, a 25% pyraclostrobin suspension (Shandong Kangqiao Biotech Co., ltd.), 430g/L mancozeb suspension (Keditwa agricultural science Co., ltd.).
The pressure of the knapsack electric sprayer is 0.5-0.6MPa, and the pressure of the knapsack electric sprayer is 3 WBD-20L. The medicine is applied once every ten days for three times. Examples 1 to 3 the preparation prepared in example was diluted 500 times and used in an amount of 1 kg per tree; the using dilution multiple of 430g/L mancozeb SC is 200 times, and the water consumption of each tree is 1 kg; the dilution multiple of 25% pyraclostrobin SC is 1000 times, and the water consumption of each tree is 6 kg.
Investigation time and number of times: investigating once before pesticide application to ensure that the test tree area is not subjected to any pesticide application treatment; the study was conducted 14 days after three applications.
Investigation time before application of drug: 2020, 4 months and 20 days; the first application time: year 2020, 4, 21 months; investigation time after three applications: 2020, 5 and 25 months.
The investigation method comprises the following steps: 2 plants in each cell are investigated at each point according to a 5-point sampling method in east, west, south and north, 4 leaves and all fruits below the top leaf of each branch are examined, and disease index and prevention and treatment effect are calculated. The grading method comprises the following steps: stage 0: the leaves and fruits have no disease spots; level 1: the area of the resin disease spots on the leaves and the fruits accounts for less than 5 percent of the area of the leaves and the fruits; and 3, level: the area of the resin disease spots on the leaves and the fruits accounts for 6 to 10 percent of the area of the leaves and the fruits; and 5, stage: the area of the resin disease spots on the leaves and the fruits accounts for 11 to 25 percent of the area of the leaves and the fruits; and 7, stage: the area of the resin disease spots on the leaves and the fruits accounts for 26 to 50 percent of the area of the leaves and the fruits; and 9, stage: the area of the resin disease spots on the leaves and the fruits accounts for more than 51 percent of the area of the leaves and the fruits.
The test complies with the quality management standard of pesticide registration test, the standard of pesticide field efficacy test, and the SOP-TM-073a citrus resinifera disease test, and the calculation formula is as follows:
Figure BDA0003839165410000081
Figure BDA0003839165410000082
TABLE 2 results of pyraclostrobin and mancozeb compounded sugar orange resinoid control
Figure BDA0003839165410000083
TABLE 3 results of pyraclostrobin and mancozeb compounded control of Or citrus resinifera disease
Figure BDA0003839165410000084
Figure BDA0003839165410000091
Test results show that the control effects of the compound preparation of mancozeb and pyraclostrobin prepared in examples 1-3 on the resin diseases of sugar oranges and citrus unshiu can reach more than 85%, and the control effects exceed the control effects of a single pyraclostrobin preparation in use. Meanwhile, the control effect on the resinifera is not very obvious when the mancozeb single agent is used, but the control effect is still far better than that of the mancozeb single agent when the pyraclostrobin and the mancozeb are compounded and used under the condition of lower application amount (calculated by effective components), so that the mancozeb single agent has an obvious synergistic effect.
Those of ordinary skill in the art will understand that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (9)

1. The application of the bactericidal composition for preventing and treating diaporthe citri is characterized in that: the sterilizing composition takes mancozeb and pyraclostrobin as main active ingredients, and the mass ratio of the mancozeb to the pyraclostrobin is (1).
2. The use of the fungicidal composition for the control of diaporthe citri according to claim 1, characterized in that: the mass ratio of mancozeb to pyraclostrobin is 1.
3. The use of the bactericidal composition for controlling diaporthe citri according to claim 2, characterized in that: the mass ratio of mancozeb to pyraclostrobin is 1-9.
4. The use of the fungicidal composition for the control of diaporthe citri according to claim 1, characterized in that: the application is to prevent or control main stems, branches, leaves and fruits of citrus plants from being infected by toxins formed by citrus aschersonia.
5. The use of the bactericidal composition for controlling diaporthe citri according to claim 4, characterized in that: the citrus plant is selected from sugar orange, wo citrus, mandarin orange, emperor citrus, ponkan citrus, eastern red citrus, and ugly citrus.
6. The use of the bactericidal composition for controlling diaporthe citri according to claim 1, characterized in that: the application is to treat citrus plants by using the bactericidal composition or treat soil where citrus plants grow by using the bactericidal composition.
7. The use of the fungicidal composition for the control of diaporthe citri according to claim 6, characterized in that: the stems, branches, leaves and fruits of citrus are treated simultaneously or separately with the germicidal composition.
8. The use of the bactericidal composition for controlling diaporthe citri according to claim 6, characterized in that: the citrus plant is subjected to root irrigation treatment by using the bactericidal composition.
9. The use of the fungicidal composition for the control of diaporthe citri according to claim 1, characterized in that: the sterilization composition is wettable powder, suspending agent and water dispersible granules which are prepared by taking mancozeb and pyraclostrobin as main effective components.
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WO2024131336A1 (en) * 2022-12-18 2024-06-27 张子勇 Ternary compound nanosuspension for prevention and control of fruit and vegetable diseases

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117794366A (en) * 2022-12-18 2024-03-29 张子勇 Nanometer suspending agent for preventing and treating fruit diseases
WO2024131336A1 (en) * 2022-12-18 2024-06-27 张子勇 Ternary compound nanosuspension for prevention and control of fruit and vegetable diseases
WO2024131338A1 (en) * 2022-12-18 2024-06-27 张子勇 Nanosuspension for fruit disease control

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