CN115266661A - Plant leaf chlorophyll fluorescence parameter detection device and method - Google Patents

Abstract

The invention provides a plant leaf chlorophyll fluorescence parameter detection device and method, wherein the device includes: a data acquisition module, a temperature measurement module and a main control processing module; the data acquisition module includes: a leaf clip, an optical fiber, a filter and a spectrometer The blade clamp is used to clamp the blade to be tested inside the blade clamp; the optical channel is used to make the light enter the blade clamp, reach the surface of the blade to be tested, and the reflected light signal is transmitted to the optical fiber; the optical fiber is used to obtain the light passing through the optical channel to be tested The light signal reflected by the leaf; the spectrometer is used to determine the irradiance of the leaf to be measured when no filter is set at the light entrance of the optical channel; when the filter is set at the light entrance of the optical channel, the fluorescence spectrum of the leaf to be measured is determined; The temperature measurement module includes: a temperature sensor; the temperature sensor is used to measure the temperature of the blade to be measured; the main control processing module is used to receive the measurement data of the blade to be measured, and determine the chlorophyll fluorescence parameter of the blade to be measured based on the measurement data of the blade to be measured.

Description

Plant leaf chlorophyll fluorescence parameter detection device and method

technical field

The invention relates to the technical field of chlorophyll analysis, in particular to a detection device and method for chlorophyll fluorescence parameters of plant leaves.

Background technique

The light energy absorbed by plants has three destinations: photosynthesis, fluorescence and heat dissipation. About 0-82% of the light is used for photosynthesis, 17.5-98% of the light is emitted in the form of heat, and 0.5-2% of the light is emitted in the form of fluorescence.

Photosynthesis is a key physiological process of vegetation, which directly reflects the nutrition and stress status of plants. However, based on photosystem II primary light energy conversion efficiency (Fv/Fm), photochemical quenching coefficient (qP), non-photochemical quenching coefficient (qN) and other chlorophyll fluorescence parameters are related to the photosynthetic ability, stress status and physiological status of plants. It is related, and more essentially reflects the process of light energy absorption and transmission by the photosystem in the process of leaf photosynthesis. It is fast, non-destructive and accurate. damage probe. Therefore, rapid and non-destructive detection of plant chlorophyll fluorescence parameters is a current research hotspot, and it is also the basis for the evaluation of crop photosynthetic ability and physiological response to stress.

Although there are devices and methods in the prior art that can accurately measure chlorophyll fluorescence parameters, they all require dark treatment of plants for a certain period of time, which consumes manpower and takes a long time to measure, which affects the efficiency of its application in the field.

Therefore, how to provide a device and method for detecting chlorophyll fluorescence parameters of plant leaves, reduce the time required for measurement, and realize efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves has become an urgent problem to be solved.

Contents of the invention

Aiming at the defects in the prior art, the present invention provides a device and method for detecting chlorophyll fluorescence parameters of plant leaves.

The invention provides a plant leaf chlorophyll fluorescence parameter detection device, comprising:

Data acquisition module, temperature measurement module and main control processing module;

The data acquisition module includes: blade clips, optical fibers, optical filters and spectrometers;

The blade clip is made of light-shielding material, and is used to clamp the blade to be tested inside the blade clip;

The blade clamp is provided with an optical channel; the optical channel is used to allow light to enter the blade clamp, reach the surface of the blade to be tested, and transmit the reflected light signal to the optical fiber;

One end of the optical fiber is perpendicular to the blade to be tested, and is arranged inside the blade clamp, for obtaining optical signals reflected by light passing through the optical channel through the blade to be tested;

The spectrometer is connected to the other end of the optical fiber to obtain the optical signal, which is used to determine the irradiance of the blade to be measured according to the optical signal when no filter is set at the light entrance of the optical channel; When the optical filter is set at the light entrance of the optical channel, according to the optical signal, the fluorescence spectrum of the leaf to be measured is determined;

The temperature-to-be-measured module includes: a temperature sensor; the temperature sensor is used to measure the temperature of the blade to be measured;

The main control processing module is used to receive the measurement data of the blade to be measured, and determine the chlorophyll fluorescence parameters of the blade to be measured based on the measurement data of the blade to be measured;

Wherein, the measurement data of the leaf to be measured includes: the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

According to the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, the optical channel includes: a first optical channel and a second optical channel; the optical fiber includes: a first optical fiber and a second optical fiber;

The filter is arranged at the light entrance of the second optical channel;

The spectrometer is connected with the first optical fiber and the second optical fiber, acquires a first optical signal through the first optical fiber, and determines the irradiance of the blade to be measured according to the first optical signal; through the The second optical fiber acquires a second light signal, and according to the second light signal, the fluorescence spectrum of the leaf to be tested is determined.

According to the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, the light-shielding material is ABS black plastic.

The present invention provides a method for detecting chlorophyll fluorescence parameters of plant leaves based on the above-mentioned detection device for chlorophyll fluorescence parameters of plant leaves, comprising: determining the chlorophyll fluorescence parameters of the leaves to be tested based on the measurement data of the leaves to be tested;

Wherein, the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the determination of the chlorophyll fluorescence parameters of the leaves to be measured based on the measurement data of the leaves to be measured specifically includes:

determining the fluorescence spectral reflectance of the leaf to be measured based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured;

Based on the irradiance of the leaf to be measured, the fluorescence spectral reflectance and the temperature of the leaf to be measured, determine the chlorophyll fluorescence parameters of the leaf to be measured.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the determination of the fluorescence spectral reflectance of the leaves to be measured based on the irradiance of the leaves to be measured and the fluorescence spectrum of the leaves to be measured specifically includes:

Based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured, according to the formula for calculating the fluorescence spectral reflectance, determine the fluorescence spectral reflectance of the leaf to be measured;

The formula for calculating the fluorescent spectral reflectance is:

Among them, R is the fluorescent spectral reflectance, L is the irradiance of the leaf to be measured, B is the dark current, and I is the irradiance of the whiteboard.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the fluorescence of the leaf to be measured is determined based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured according to the calculation formula of the fluorescence spectrum reflectance. Before the spectral reflectance step, also include:

Carrying out whiteboard calibration based on the plant leaf chlorophyll fluorescence parameter detection device, and obtaining the irradiance of the whiteboard;

When the plant leaf chlorophyll fluorescence parameter detection device does not clamp the leaf to be tested, the dark current is collected.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the chlorophyll fluorescence parameters of the leaves to be measured are determined based on the irradiance of the leaves to be measured, the fluorescent spectral reflectance and the temperature of the leaves to be measured, Specifically include:

Based on the temperature of the leaf to be measured, calculate the heat dissipation energy of the leaf to be measured, and determine the corrected fluorescence intensity;

determining a compensated fluorescent spectral reflectance based on the fluorescent spectral reflectance and the corrected fluorescent intensity;

Based on the compensated fluorescence spectral reflectance, the chlorophyll fluorescence parameter of the leaf to be tested is determined.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the determination of the compensated fluorescence spectral reflectance based on the irradiance of the leaf to be measured, the fluorescent spectral reflectance and the corrected fluorescent intensity, specifically includes:

Based on the irradiance of the leaves to be measured, the fluorescence inversion is carried out according to the Fraunhofer dark-line method, and the fluorescence intensity after the inversion is determined;

Based on the inverted fluorescence intensity and the fluorescent spectral reflectance, a compensated fluorescent spectral reflectance is determined.

According to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the determination of the chlorophyll fluorescence parameters of the leaves to be tested based on the compensated fluorescence spectral reflectance specifically includes:

Determine the modified fluorescence ratio index based on the compensated fluorescence spectral reflectance; wherein, the modified fluorescence ratio index is used to represent the ratio of the compensated fluorescence reflectance of the leaves to be measured in different bands;

Based on the modified fluorescence ratio index, the chlorophyll fluorescence parameter of the leaf to be tested is determined.

The device and method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention calculate the chlorophyll fluorescence parameters based on the obtained radiance information, fluorescence spectrum and leaf temperature of plant leaves, and realize real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves detection.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.

Fig. 1 is one of the structural schematic diagrams of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention;

Fig. 2 is one of partial structural schematic diagrams of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention;

Fig. 3 is the second schematic diagram of the partial structure of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention;

Fig. 4 is the second structural diagram of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention;

5 is a schematic diagram of the structure and principle of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention;

Fig. 6 is the flowchart of the method for detecting the fluorescence parameters of plant leaf chlorophyll provided by the present invention;

7 is a schematic flow chart of a method for detecting fluorescence parameters of plant leaf chlorophyll provided by the present invention;

Fig. 8 is the Fraunhofer chlorophyll fee dark line inversion curve diagram of solar irradiance provided by the present invention;

Fig. 9 is the irradiance curve of the Fraunhofer chlorophyll fee dark line inversion provided by the present invention;

FIG. 10 is a schematic diagram of the physical structure of the electronic device provided by the present invention.

Reference signs:

110: data acquisition module; 120: temperature measurement module;

130: main control processing module; 210: blade clip;

220: optical fiber; 230: optical filter;

240: optical channel; 250: temperature sensor;

310: first optical channel; 320: second optical channel;

330: first optical fiber; 340: second optical fiber;

410: spectrometer; 420: power supply module;

430: Touch the display screen.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

The devices used to detect chlorophyll fluorescence parameters in the prior art include the PAM-2500 portable modulated chlorophyll fluorescence instrument produced by German WALZ company and the FluorCam closed chlorophyll fluorescence imaging system produced by Beijing Yiketai Ecological Technology Co., Ltd.

The PAM-2500 portable modulated chlorophyll fluorescence instrument produced by German WALZ company is composed of LED light source, detector, and control unit. The middle part of the leaf is clamped with a dark adaptation clip and the leaf is fully dark adapted for 30 minutes. After that, a certain intensity and time saturation pulse light is applied. Measure light and actinic light to obtain a range of chlorophyll fluorescence parameters.

The FluorCam closed-type chlorophyll fluorescence imaging system produced by Beijing Yiketai Ecological Technology Co., Ltd. includes: excitation light source, filter, detector, computer and control module, etc., which can obtain the chlorophyll fluorescence signal of plant leaves or canopy, and also need to go through Only after 15-30min of dark treatment can a fast kinetic curve and a series of chlorophyll fluorescence parameters be obtained.

The above instrument can accurately measure the chlorophyll fluorescence parameters, but it needs dark treatment for a certain period of time, which consumes manpower and takes a long time to measure, which affects the efficiency of its application in the field.

There are following problems in prior art:

(1) The current existing technology only uses the reflectance spectrum of the leaves, and mainly uses the vegetation index that can detect the fluorescence information to estimate the chlorophyll fluorescence parameters, such as the reflectance ratio index, but the fluorescence signal is relatively weak and accounts for about 5% of the reflected light, indirectly The detection accuracy of weak fluorescence signal extracted from reflectance spectrum is low, and it cannot essentially reflect the mechanism of chlorophyll fluorescence.

(2) The emitted fluorescence is mainly superimposed with the plant reflection spectrum in the red light and red edge area of 650-800nm. Since chlorophyll absorbs red light, the emitted fluorescence is reabsorbed by chlorophyll, which greatly affects the instrument's red light band range to chlorophyll. The detection of fluorescence leads to a large error in the measurement of the single-point fluorescence spectral reflectance in the red light range.

(3) About 17.5-98% of the light energy absorbed by plants is used for heat dissipation, which affects the detection of fluorescence signals of the instrument, but the heat dissipation of crops can also indirectly reflect the fluorescence intensity of crops, and the heat dissipation of crop leaves Large interference in chlorophyll fluorescence detection.

In view of the above three problems, the present invention provides a device and method for detecting chlorophyll fluorescence parameters of plant leaves. The radiance information, fluorescence spectrum and leaf temperature of plant leaves are obtained, and the chlorophyll fluorescence parameters are calculated to realize real-time, Non-destructive efficient and accurate detection.

Fig. 1 is one of the structural schematic diagrams of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention. As shown in Fig. 1, the present invention provides a plant leaf chlorophyll fluorescence parameter detection device, including: a data acquisition module, a temperature measurement module and a main control processing module;

The data acquisition module includes: blade clips, optical fibers, optical filters and spectrometers;

The blade clip is made of light-shielding material, and is used to clamp the blade to be tested inside the blade clip;

The blade clamp is provided with an optical channel; the optical channel is used to allow light to enter the blade clamp, reach the surface of the blade to be tested, and transmit the reflected light signal to the optical fiber;

One end of the optical fiber is perpendicular to the blade to be tested, and is arranged inside the blade clamp, for obtaining optical signals reflected by light passing through the optical channel through the blade to be tested;

The spectrometer is connected to the other end of the optical fiber to obtain the optical signal, which is used to determine the irradiance of the blade to be measured according to the optical signal when no filter is set at the light entrance of the optical channel; When the optical filter is set at the light entrance of the optical channel, according to the optical signal, the fluorescence spectrum of the leaf to be measured is determined;

The temperature-to-be-measured module includes: a temperature sensor; the temperature sensor is used to measure the temperature of the blade to be measured;

The main control processing module is used to receive the measurement data of the blade to be measured, and determine the chlorophyll fluorescence parameters of the blade to be measured based on the measurement data of the blade to be measured;

Wherein, the measurement data of the leaf to be measured includes: the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

Specifically, Fig. 2 is one of the partial structural diagrams of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention. As shown in Fig. 2, the plant leaf chlorophyll fluorescence parameter detection device includes: a data acquisition module 110, a temperature measurement module 120 and a main control The processing module 130 ; wherein, the data acquisition module 110 includes: a blade clip 210 , an optical fiber 220 , an optical filter 230 and a spectrometer; the temperature-to-be-measured module 120 includes: a temperature sensor 250 .

The blade clamp 210 is made of light-shielding material, and can be divided into an upper hinge and a lower hinge, and is used to hold the blade clamp 210 to be tested inside the blade clamp 210 (clamped in the middle area of the upper hinge and the lower hinge).

The blade clamp 210 is provided with an optical channel 240 , and the optical channel 240 is used to allow light to enter the blade clamp 210 and reach the surface of the blade to be tested, and the reflected light signal is transmitted to the optical fiber 220 . The diameter of the optical channel 240 where the light source enters and where the optical fiber 220 is located is the same, for example, both are set to 12.8 mm.

One end of the optical fiber 220 is perpendicular to the blade to be tested, and is arranged inside the blade clamp 210 for acquiring light signals reflected by the light passing through the optical channel 240 and the blade to be tested.

The spectrometer is connected to the other end of the optical fiber 220 to obtain optical signals. In this device, the installation position of the filter 230 can be moved. When the optical filter 230 is not set at the light entrance of the optical channel 240, the spectrometer acquires the optical signal through the optical fiber 220, and determines the irradiance of the blade to be measured according to the optical signal.

When the filter 230 is set at the light entrance of the optical channel 240, the light entrance of the optical channel 240 is completely blocked by the filter 230. When the filter 230 is set, the excitation light source of chlorophyll fluorescence is filtered out, and what is obtained is filtered The optical signal of part of the band is obtained to obtain the fluorescence spectrum of the leaf to be measured by separating the reflection spectrum. Wherein, the filter 230 can be a 650nm short-pass filter 230 .

The temperature sensor 250 is used to measure the temperature of the blade to be tested, and is set close to the blade to be tested, which reduces the interference of the external environment on the measured temperature and can accurately measure the surface temperature of the blade.

The main control processing module 130 is used to receive the measurement data of the leaf to be tested collected by the spectrometer and the temperature sensor 250, and determine the chlorophyll fluorescence parameters of the leaf to be tested based on the measurement data of the leaf to be tested. Wherein, the measurement data of the leaf to be tested includes: the irradiance of the leaf to be tested, the fluorescence spectrum of the leaf to be tested, and the temperature of the leaf to be tested.

It can be understood that, in addition to detecting leaves, the detection device provided by the present invention can also be adaptively applied to detect other tissues of plants, which is not limited in the present invention.

The plant leaf chlorophyll fluorescence parameter detection device provided by the present invention can directly collect the fluorescence spectrum of leaves by designing a new type of leaf clip to isolate the interference of external light intensity, effectively solving the problem of low detection accuracy of indirect extraction of fluorescence signals from reflection spectrum, which cannot essentially reflect chlorophyll The problem of the mechanism of fluorescence generation. A patch temperature sensor is added to the leaf clip to measure the surface temperature of the leaf and obtain the information of the heat released by the leaf, which can indirectly reflect the intensity of the fluorescence signal released by the leaf. It can solve the problem that the fluorescence signal is reabsorbed in the red light band to extract the weak fluorescence signal, which causes a large error in the measurement of the single-point fluorescence spectral reflectance. Through the obtained radiance information, fluorescence spectrum and leaf temperature of plant leaves, the fluorescence spectrum reflectance is compensated and corrected, and the chlorophyll fluorescence parameters are further calculated to realize real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves.

Optionally, according to the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, the optical channel includes: a first optical channel and a second optical channel; the optical fiber includes: a first optical fiber and a second optical fiber;

The filter is arranged at the light entrance of the second optical channel;

The spectrometer is connected with the first optical fiber and the second optical fiber, acquires a first optical signal through the first optical fiber, and determines the irradiance of the blade to be measured according to the first optical signal; through the The second optical fiber acquires a second light signal, and according to the second light signal, the fluorescence spectrum of the leaf to be tested is determined.

Specifically, Fig. 3 is the second schematic diagram of the partial structure of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention. As shown in Fig. 3, in order to more conveniently measure the optical signal, the leaf clip is designed as a dual-channel leaf clip. Wherein, the optical channels include: a first optical channel 310 and a second optical channel 320 . The first optical channel 310 and the second optical channel 320 are arranged on the upper hinge of the blade clip and separated from each other, and the lower hinge is used to fix the blade.

Correspondingly, the optical fiber includes: a first optical fiber 330 and a second optical fiber 340 . The filter is arranged at the light entrance of the second optical channel 320 and completely covers the entrance of the second optical channel 320 .

The spectrometer 410 is connected with the first optical fiber 330 and the second optical fiber 340 , acquires the first optical signal through the first optical fiber 330 , and determines the irradiance of the blade to be measured according to the first optical signal. The second optical signal is obtained through the second optical fiber 340, and the fluorescence spectrum of the leaf to be measured is determined according to the second optical signal.

The plant leaf chlorophyll fluorescence parameter detection device provided by the present invention can directly collect the irradiance and fluorescence spectrum of the leaves by designing a new double-channel leaf clip to isolate the interference of external light intensity, and effectively solve the detection accuracy of indirect extraction of fluorescence signals from the reflection spectrum Low, it cannot essentially reflect the problem of the mechanism of chlorophyll fluorescence. A patch temperature sensor is added to the leaf clip to measure the surface temperature of the leaf and obtain the information of the heat released by the leaf, which can indirectly reflect the intensity of the fluorescence signal released by the leaf. It can solve the problem that the fluorescence signal is reabsorbed in the red light band to extract the weak fluorescence signal, which causes a large error in the measurement of the single-point fluorescence spectral reflectance. Through the obtained radiance information, fluorescence spectrum and leaf temperature of plant leaves, the fluorescence spectrum reflectance is compensated and corrected, and the chlorophyll fluorescence parameters are further calculated to realize real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves.

Optionally, according to the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, the light-shielding material is ABS black plastic.

Specifically, in the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, the light-shielding material is preferably ABS black plastic.

ABS plastic is a terpolymer of acrylonitrile (A), butadiene (B), and styrene (S). ABS plastic has the common properties of the three components. A makes it resistant to chemical corrosion and Heat, and has a certain surface hardness, B makes it have high elasticity and toughness, S makes it have the processing and molding characteristics of thermoplastics and improve electrical properties.

ABS black plastic is easy to process, corrosion-resistant, and light in weight, which can well meet the experimental requirements (for example, the distance between the front end of the clip and the shaft is set to 50mm, and the clip can clamp any part of the crop blade), used to make blades The clip can effectively isolate the interference of external light intensity and make the detection process easy to operate.

The plant leaf chlorophyll fluorescence parameter detection device provided by the present invention calculates the chlorophyll fluorescence parameters based on the obtained plant leaf radiance information, fluorescence spectrum and leaf temperature, and realizes real-time, non-destructive, efficient and accurate detection of the plant leaf chlorophyll fluorescence parameters . Among them, the light-shielding material for making the blade clip is ABS black plastic, which makes the detection process easy to operate on the basis of effectively isolating the interference of external light intensity.

Fig. 4 is the second structural diagram of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, and Fig. 5 is a structural schematic diagram of the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention, as shown in Fig. 4 and Fig. 5, for plant leaf chlorophyll The hardware design of the fluorescence parameter detection device in the actual application process is described in detail:

The plant leaf chlorophyll fluorescence parameter detection device includes: a main control processing module 130 , a leaf clip 210 , a spectrometer 410 , a power supply module 420 and a touch screen 430 .

FIG. 5 is a detailed schematic diagram of the device, wherein the power supply module 420 supplies power to the spectrometer 410 , the temperature measurement module 120 , and the main control processing module 130 .

The main control processing module uses Raspberry Pi Zero W, and uses a 1GHz ARM11 core architecture processor. The data acquisition module is mainly composed of a dual-channel leaf clip, a bifurcated optical fiber and a micro-spectrometer. It mainly measures the fluorescence spectrum and irradiance information of the leaves. The short-pass filter cut-off wavelength is 650nm, and the wavelength range of the fluorescence spectrum obtained by the micro-spectrometer is 650 nm. -800nm, the concentration range of fluorescence bands, and the spectral resolution is 1nm. The temperature measurement module uses a patch thermocouple temperature sensor, and the WZP-pt100 patch thermocouple sensor is used for measurement. Paste on the surface of the blade, press the button to measure the surface temperature of the blade; the control software and display module mainly realize the data acquisition control software operation, data storage and processing, and calling the detection model.

It should be noted that the design of the above hardware is only used as a specific example to illustrate the plant leaf chlorophyll fluorescence parameter detection device provided by the present invention. In the actual application process, in the present invention, the specific structure and The shape, the installation position of each module, and the specific selection of hardware in each module. For the software setup of the system, it matches the hardware design. The specific settings of hardware design and software design can be adjusted according to actual needs, which is not limited in this embodiment.

Fig. 6 is the flowchart of the method for detecting the chlorophyll fluorescence parameters of plant leaves provided by the present invention. As shown in Fig. 6, the present invention provides a method for detecting the chlorophyll fluorescence parameters of plant leaves based on the above-mentioned device for detecting the chlorophyll fluorescence parameters of plant leaves, including:

Step S1, based on the measurement data of the leaf to be tested, determine the chlorophyll fluorescence parameters of the leaf to be tested;

Wherein, the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

Specifically, in step S1, the chlorophyll fluorescence parameter of the leaf to be tested is determined based on the measurement data of the leaf to be tested detected by the plant leaf chlorophyll fluorescence parameter detection device.

Among them, the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

It can be understood that when determining the chlorophyll fluorescence parameters of the leaves to be tested according to the irradiance of the leaves to be tested, the fluorescence spectrum of the leaves to be tested, and the temperature of the leaves to be tested, the data test data can be used to fit the chlorophyll fluorescence parameters and the leaf irradiance , the relationship between leaf fluorescence spectrum and leaf temperature, determine the fitting formula, and determine the chlorophyll fluorescence parameters according to the formula. In addition, a neural network model can also be constructed to obtain a chlorophyll fluorescence parameter determination model through a large amount of data training, and the chlorophyll fluorescence parameter can be determined through the neural network model. The specific method can be selected according to actual needs, which is not limited in the present invention.

The method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention calculates the chlorophyll fluorescence parameters based on the acquired radiance information, fluorescence spectrum and leaf temperature of plant leaves, and realizes real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves .

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided in the present invention, the determination of the chlorophyll fluorescence parameters of the leaves to be tested based on the measurement data of the leaves to be tested specifically includes:

determining the fluorescence spectral reflectance of the leaf to be measured based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured;

Based on the irradiance of the leaf to be measured, the fluorescence spectral reflectance and the temperature of the leaf to be measured, determine the chlorophyll fluorescence parameters of the leaf to be measured.

Specifically, Fig. 7 is a schematic flowchart of a method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention. As shown in Fig. 7, based on the measurement data of the leaves to be measured, the chlorophyll fluorescence parameters of the leaves to be tested are determined, specifically including:

Based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured obtained by the detection device, the reflectance of the fluorescence spectrum of the leaf to be measured is calculated and determined.

Based on the calculated fluorescent spectral reflectance, as well as the detected irradiance and temperature of the leaf to be tested, the chlorophyll fluorescence parameters of the leaf to be tested are determined.

Further, it can be understood that after determining the chlorophyll fluorescence parameter, it is determined that the chlorophyll fluorescence parameter is within a normal threshold range to ensure the accuracy of the calculation result.

In the method for detecting the chlorophyll fluorescence parameters of plant leaves provided by the invention, the radiance information, fluorescence spectrum and leaf temperature of the plant leaves are obtained, and the irradiance of the leaves to be measured and the fluorescence spectrum of the leaves to be measured obtained by the detection device are calculated and determined. The fluorescence spectral reflectance of leaves reduces the measurement error of fluorescence spectral reflectance, compensates and corrects the fluorescence spectral reflectance, further calculates the chlorophyll fluorescence parameters, and realizes real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves.

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided in the present invention, the fluorescence spectral reflectance of the leaf to be measured is determined based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured, specifically include:

Based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured, according to the formula for calculating the fluorescence spectral reflectance, determine the fluorescence spectral reflectance of the leaf to be measured;

The formula for calculating the fluorescent spectral reflectance is:

Among them, R is the fluorescent spectral reflectance, L is the irradiance of the leaf to be measured, B is the dark current, and I is the irradiance of the whiteboard.

Specifically, based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured, the fluorescence spectral reflectance of the leaf to be measured is determined, specifically including:

The irradiance of the leaf to be tested, the fluorescence spectrum of the leaf to be tested, the dark current and the irradiance of the white board are substituted into the calculation formula of the fluorescence spectral reflectance to determine the fluorescence spectral reflectance of the leaf to be tested.

The formula for calculating the fluorescence spectral reflectance is:

Among them, R is the fluorescent spectral reflectance, L is the irradiance of the leaf to be measured, B is the dark current, and I is the irradiance of the whiteboard.

Compared with the existing technology, based on the irradiance of the whiteboard and the irradiance of the leaves to be measured, the chlorophyll fluorescence intensity (fluorescence spectrum of the leaves to be measured) is introduced to correct the reflectance of the fluorescence spectrum, which reduces the fluorescence signal being absorbed by chlorophyll in the red light band. The influence of reabsorption reduces the measurement error of single-point fluorescence spectral reflectance in the red light range.

The method for detecting the chlorophyll fluorescence parameters of plant leaves provided by the present invention combines the radiance information, fluorescence spectrum and leaf temperature of plant leaves obtained by the detection device with the irradiance of the leaves to be measured and the fluorescence spectrum of the leaves to be measured, combined with whiteboard irradiation degree and dark current, calculate and determine the fluorescent spectral reflectance of the leaves to be tested, reduce the measurement error of the fluorescent spectral reflectance, compensate and correct the fluorescent spectral reflectance, further calculate the chlorophyll fluorescence parameters, and realize the real-time and non-linear analysis of the chlorophyll fluorescence parameters of the plant leaves. Destructive efficient and accurate detection.

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided in the present invention, the irradiance of the leaves to be measured and the fluorescence spectrum of the leaves to be measured are determined according to the calculation formula of the fluorescence spectrum reflectance. Before the step of measuring the fluorescence spectral reflectance of the blade, it also includes:

Carrying out whiteboard calibration based on the plant leaf chlorophyll fluorescence parameter detection device, and obtaining the irradiance of the whiteboard;

When the plant leaf chlorophyll fluorescence parameter detection device does not clamp the leaf to be tested, the dark current is collected.

Specifically, as shown in Figure 7, before the step of determining the fluorescent spectral reflectance of the leaf to be measured based on the irradiance of the leaf to be measured and the fluorescence spectrum of the leaf to be measured, according to the calculation formula of the fluorescence spectral reflectance, it also includes:

The whiteboard is calibrated based on the plant leaf chlorophyll fluorescence parameter detection device, and the whiteboard is clamped at the position where the leaf to be tested is clamped, and the irradiance of the whiteboard (that is, the solar irradiance) is obtained through an optical channel without a filter.

When the plant leaf chlorophyll fluorescence parameter detection device does not clamp the leaf to be measured, the irradiance at this time, that is, the dark current, is collected by a spectrometer.

The method for detecting the chlorophyll fluorescence parameters of plant leaves provided by the present invention combines the radiance information, fluorescence spectrum and leaf temperature of plant leaves obtained by the detection device with the irradiance of the leaves to be measured and the fluorescence spectrum of the leaves to be measured, combined with whiteboard irradiation degree and dark current, calculate and determine the fluorescent spectral reflectance of the leaves to be tested, reduce the measurement error of the fluorescent spectral reflectance, compensate and correct the fluorescent spectral reflectance, further calculate the chlorophyll fluorescence parameters, and realize the real-time and non-linear analysis of the chlorophyll fluorescence parameters of the plant leaves. Destructive efficient and accurate detection.

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided in the present invention, the irradiance of the leaves to be tested is determined based on the irradiance of the leaves to be tested, the fluorescence spectral reflectance and the temperature of the leaves to be tested. Chlorophyll fluorescence parameters, including:

Based on the temperature of the leaf to be measured, calculate the heat dissipation energy of the leaf to be measured, and determine the corrected fluorescence intensity;

determining a compensated fluorescent spectral reflectance based on the fluorescent spectral reflectance and the corrected fluorescent intensity;

Based on the compensated fluorescence spectral reflectance, the chlorophyll fluorescence parameter of the leaf to be tested is determined.

Specifically, since the blade releases heat through long-wave infrared radiation (LR _em ), the long-wave infrared radiation released by the blade is proportional to the fourth power of its absolute temperature (T) and emissivity (ε), using the following formula:

LR _em =ε·σ·T ⁴

Among them, σ is a ratio constant, that is, the Stefan-Boltzmann constant; the theoretical value of ε is 1, and the value of ε of objects in reality varies between 0 and 1. The corrected fluorescence intensity (the fluorescence intensity of crop leaves corrected by heat dissipation radiation) _Fheat is:

F _heat = K*(1-k*LR _em -m) = K/(1-k*ε·σ·T ⁴ -m);

Among them, K and k are proportional coefficients, and m is between 0 and 1.

Based on the fluorescence spectral reflectance and the temperature of the leaf to be measured, determine the chlorophyll fluorescence parameters of the leaf to be measured, specifically including:

Substitute the collected temperature of the leaf to be measured into the above corrected fluorescence intensity calculation formula to determine the corrected fluorescence intensity.

Based on the fluorescent spectral reflectance collected by the device and the corrected fluorescence intensity, the fluorescent spectral reflectance is compensated, and the compensated fluorescent spectral reflectance is determined.

Based on the compensated fluorescence spectral reflectance, the chlorophyll fluorescence parameters of the leaves to be tested were determined.

The method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention can indirectly reflect the intensity of fluorescence signal released by leaves by obtaining the radiance information, fluorescence spectrum and leaf temperature of plant leaves, and according to the surface temperature of leaves, the information of heat released by leaves can reflect the intensity of fluorescence signals released by leaves. The temperature compensates and corrects the reflectance of the fluorescence spectrum, and further calculates the chlorophyll fluorescence parameters. It can effectively solve the problem of large interference of heat dissipation of crop leaves in the detection of chlorophyll fluorescence, and realize real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves.

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention, the compensated fluorescence spectral reflectance is determined based on the irradiance of the leaf to be measured, the fluorescent spectral reflectance and the corrected fluorescence intensity , including:

Based on the irradiance of the leaves to be measured, the fluorescence inversion is carried out according to the Fraunhofer dark-line method, and the fluorescence intensity after the inversion is determined;

Based on the inverted fluorescence intensity and the fluorescent spectral reflectance, a compensated fluorescent spectral reflectance is determined.

Specifically, the vegetation reflection irradiance spectrum measured by the spectrometer under natural lighting conditions includes the emission spectrum of solar-induced fluorescence and the reflection spectrum of leaves to incident light. Due to the absorption of the solar atmosphere and the earth's atmosphere, there are many small dark lines in the solar irradiance spectrum reaching the surface, called Fraunhofer dark lines.

At the solar Fraunhofer dark line or the Earth's oxygen absorption line, the proportion of fluorescence to the total radiant energy is relatively large. Within the wavelength range covered by the fluorescence spectrum, there are multiple absorption lines of the sun or the earth's atmosphere that can be used for chlorophyll fluorescence detection. In the present invention, fluorescence inversion is performed according to the Fraunhofer dark line of the sun, and the extracted fluorescence signal is used to correct the interference of chlorophyll reabsorption on the fluorescence spectrum.

Based on the irradiance of the leaves to be measured, the fluorescence spectral reflectance and the corrected fluorescence intensity, determine the compensated fluorescent spectral reflectance, specifically including:

Based on the irradiance of the leaves to be measured, the fluorescence inversion was carried out according to the Fraunhofer dark line method, and the inversion fluorescence intensity was determined.

Since the Fraunhofer dark lines formed by the two oxygen absorptions at 687nm and 760nm have obvious characteristics and strong fluorescence, the present invention will be described by taking the use of these two wave bands as an example. Figure 8 is the Fraunhofer dark lines provided by the present invention Inverting the solar irradiance curve, Fig. 9 is the Fraunhofer and chlorophyll fee dark line inversion curve of the blade irradiance to be measured provided by the present invention, as shown in Fig. 8 and Fig. 9, it is determined that the two oxygen absorption bands are O ₂ -B (687nm) and _O2 -A (760nm).

Among them, F _in is the fluorescence intensity of the band inside the absorption line, ω _left and ω _right are the weights occupied by the left and right reference bands of the absorption line, and λ _left , λ _in , λ _right are the right, inner and left bands of the absorption line Wavelength, I _left , I _in , I _right are the solar irradiance spectral intensity (whiteboard irradiance spectral intensity) on the left, inner and right of the absorption line, L _left , L _in , L _right are the left, inner and right of the absorption line The vegetation canopy reflectance radiance spectral intensity of .

It can be understood that the exact value of the absorption band will change according to the resolution of the spectrometer, and it is roughly around 687 and 760nm.

Taking the selected light wavelengths of 761nm and 687nm as an example, the fluorescence intensity F of crop leaves extracted for the Fraunhofer dark line is as follows:

F＝k ₁ *F761+k ₂ *F687

Among them, k ₁ and k ₂ are the coefficients of each parameter, and F761 and F687 represent the fluorescence intensity at 761nm and 687nm bands.

It can be understood that the calculation method based on the fluorescence intensity at the 761nm and 687nm bands in this scheme is only used as a specific example to describe the scheme of the present invention in detail, and the wavelength of light selected in the actual application process can be based on actual needs. adjustments, which are not limited in the present invention.

The calculation formula of the compensated fluorescence spectral reflectance MR is:

MR＝R±F

Substitute the inverted fluorescence intensity and fluorescence spectral reflectance into the compensated fluorescence spectral reflectance MR calculation formula to determine the compensated fluorescent spectral reflectance.

The method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention obtains the radiance information, fluorescence spectrum and leaf temperature of plant leaves, compensates and corrects the reflectance of the fluorescence spectrum, further calculates the chlorophyll fluorescence parameters, and directly obtains the plant fluorescence spectrum and heat consumption Scattered information, and compensate for the reflectance of the fluorescence spectrum in the red light band range to improve the detection accuracy of chlorophyll fluorescence parameters, and realize real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters of plant leaves.

Optionally, according to the method for detecting chlorophyll fluorescence parameters of plant leaves provided in the present invention, the determination of the chlorophyll fluorescence parameters of the leaves to be tested based on the compensated fluorescence spectral reflectance specifically includes:

Determine the modified fluorescence ratio index based on the compensated fluorescence spectral reflectance; wherein, the modified fluorescence ratio index is used to represent the ratio of the compensated fluorescence reflectance of the leaves to be measured in different bands;

Based on the modified fluorescence ratio index, the chlorophyll fluorescence parameter of the leaf to be tested is determined.

Specifically, based on the compensated fluorescence spectral reflectance, the chlorophyll fluorescence parameters of the leaves to be tested are determined, specifically including:

Based on the compensated fluorescence spectral reflectance, a modified fluorescence ratio index is determined, and based on the modified fluorescence ratio index, the chlorophyll fluorescence parameters of the leaves to be tested are determined. Among them, the modified fluorescence ratio index is used to represent the ratio of the compensated fluorescence reflectance of leaves in different bands.

The formula for calculating the modified fluorescence ratio index is:

Among them, band1, band2, band3 and band4 are the bands selected for calculation, and R _band1 , R _band2 , R _band3 and R _band4 are the fluorescence spectral reflectance at each band (wherein, the numerator and denominator are the fluorescence spectrum after compensation of the corresponding band Reflectivity).

Taking two fluorescence spectrum peak positions 685nm and 740nm and bands 655nm and 720nm less affected by fluorescence as examples, the construction of the modified fluorescence ratio index is explained. The specific formula is as follows:

Among them, k ₃ , k ₄ , k ₅ , and k ₆ are the coefficients of each parameter, and R685, R655, R740, and R720 represent the fluorescence spectral reflectance at 685nm, 655nm, 740nm, and 720nm bands.

The fluorescence intensity extracted from the Fraunhofer dark line, the fluorescence intensity corrected for heat dissipation, and the modified fluorescence ratio index were combined.

The calculation formula of chlorophyll fluorescence parameters is as follows:

y＝k ₇ *F _heat +k ₈ *F+k ₉ *MRVI(band1,band2)+k ₁₀ *MRVI(band3,band4)

Enter the specific value to determine the chlorophyll fluorescence parameter as:

y＝k ₇ *F _heat +k ₈ *F+k ₉ *MRVI(685,655)+k ₁₀ *MRVI(740,720)

Among them, y represents the chlorophyll fluorescence parameters of crop leaves, k7, k8, k9, and k10 are the coefficients of each parameter, F is the fluorescence intensity of crop leaves extracted from the Fraunhofer dark line, and MRVI is the adjusted fluorescence ratio index.

It can be understood that in this scheme, two fluorescent spectrum peak positions 685nm and 740nm and wave bands 655nm and 720nm less affected by fluorescence are selected to construct a modified fluorescence ratio index method, which is only used as a specific example for the scheme of the present invention To describe in detail, the wavelength of light selected in the actual application process can be adjusted according to actual needs, which is not limited in the present invention.

The method for detecting chlorophyll fluorescence parameters of plant leaves provided by the present invention can measure and extract chlorophyll fluorescence signals more essentially by directly obtaining the fluorescence spectrum of leaves, which reduces the measurement time and improves the application and high-throughput testing in field environments. efficiency. Through the obtained radiance information, fluorescence spectrum and leaf temperature of plant leaves, the fluorescence intensity of crop leaves corrected by heat dissipation radiation is calculated, which reduces the problem that heat dissipation of crop leaves interferes greatly in the detection of chlorophyll fluorescence. The adjusted ratio index, as a parameter of the chlorophyll fluorescence parameter prediction model, makes the prediction result of the model more accurate than a single fluorescence intensity value, and realizes real-time, non-destructive, efficient and accurate detection of the chlorophyll fluorescence parameters of plant leaves.

In order to describe the technical solution of the present invention more clearly, the present invention is described in detail in conjunction with specific steps (in conjunction with Figure 3):

(1) Turn on the power switch of the plant leaf chlorophyll fluorescence parameter detection device, supply power to each module, and start preparing for data collection.

(2) Press the button of the patch thermocouple temperature sensor, the measurement result will be displayed and saved on the display screen, and the fluorescence intensity F _heat of the crop leaves corrected for heat dissipation will be calculated at the same time.

(3) Carry out the whiteboard calibration, put the whiteboard in the blade clip, utilize the first optical channel and the first optical fiber to obtain the whiteboard reflected light irradiance value, that is, the solar irradiance, denoted as 1.

(4) Collect the dark current of the instrument, the measuring head does not clamp the sample, and collect the irradiance (dark current) B at this time.

(5) The clip is closed to clamp the sample, and the irradiance of the crop leaves is collected by using the first optical channel and the first optical fiber, and the output value of the sensor is recorded, which is denoted as L.

(6) Using the second optical channel and the second optical fiber to collect the fluorescence spectrum of the crop, and calculate the reflectance R of the fluorescence spectrum.

(7) Based on the obtained leaf temperature, calculate the fluorescence intensity F687 at O ₂ -B (687nm) and the fluorescence intensity F761 at O ₂ -A (761nm). The calculated fluorescence intensity was used to correct and adjust the fluorescence ratio index to obtain MRVI (685, 655) and MRVI (740, 720) to eliminate the influence of chlorophyll reabsorption on the measurement, so as to improve the prediction accuracy of the model. Embedding the obtained model into the system software can display the leaf chlorophyll fluorescence parameters in real time.

It should be noted that the above steps are only used as a specific example to illustrate the method for detecting the chlorophyll fluorescence parameters of plant leaves provided by the present invention, and adaptive adjustments can be made during practical application, which is not limited by the present invention.

FIG. 10 is a schematic diagram of the physical structure of the electronic device provided by the present invention. As shown in FIG. 10, the electronic device may include: a processor (processor) 101, a communication interface (communication interface) 102, a memory (memory) 103 and a communication bus (bus) 104 , wherein, the processor 101 , the communication interface 102 , and the memory 103 communicate with each other through the communication bus 104 . The processor 101 can call the logic instructions in the memory 103 to execute the above method for detecting the chlorophyll fluorescence parameters of the plant leaves, including: determining the chlorophyll fluorescence parameters of the leaves to be tested based on the measurement data of the leaves to be tested; wherein, the The irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

In addition, the above-mentioned logic instructions in the memory 103 may be realized in the form of software functional units and may be stored in a computer-readable storage medium when sold or used as an independent product. Based on this understanding, the essence of the technical solution of the present invention or the part that contributes to the prior art or the part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including Several instructions are used to make a computer device (which may be a personal computer, a server, or a network device, etc.) execute all or part of the steps of the method described in each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-only Memory), random access memory (RAM, Random Access Memory), magnetic disk or optical disk, etc., which can store program codes. .

On the other hand, an embodiment of the present invention also provides a computer program product, the computer program product includes a computer program stored on a non-transitory computer-readable storage medium, the computer program includes program instructions, and when the program instructions When executed by a computer, the computer can execute the method for detecting the chlorophyll fluorescence parameter of the plant leaf provided by the method embodiments above, the method comprising: determining the chlorophyll fluorescence parameter of the leaf to be tested based on the measurement data of the leaf to be tested; wherein , the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured, and the temperature of the leaf to be measured.

In yet another aspect, an embodiment of the present invention also provides a non-transitory computer-readable storage medium, on which a computer program is stored, and when the computer program is executed by a processor, it is implemented to perform the functions provided by the above-mentioned embodiments to perform plant leaf chlorophyll A fluorescence parameter detection method, the method includes: based on the measurement data of the leaf to be measured, determining the chlorophyll fluorescence parameter of the leaf to be measured; wherein, the irradiance of the leaf to be measured, the fluorescence spectrum of the leaf to be measured and the Describe the temperature of the leaf to be measured.

The device embodiments described above are only illustrative, and the units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in One place, or it can be distributed to multiple network elements. Part or all of the modules can be selected according to actual needs to achieve the purpose of the solution of this embodiment. It can be understood and implemented by those skilled in the art without any creative effort.

Through the above description of the implementations, those skilled in the art can clearly understand that each implementation can be implemented by means of software plus a necessary general hardware platform, and of course also by hardware. Based on this understanding, the essence of the above technical solution or the part that contributes to the prior art can be embodied in the form of software products, and the computer software products can be stored in computer-readable storage media, such as ROM/RAM, magnetic discs, optical discs, etc., including several instructions to make a computer device (which may be a personal computer, server, or network device, etc.) execute the methods described in various embodiments or some parts of the embodiments.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims (10)

1. A plant leaf chlorophyll fluorescence parameter detection device, characterized by includes: the device comprises a data acquisition module, a temperature measurement module and a main control processing module;

the data acquisition module comprises: the device comprises a blade clamp, an optical fiber, an optical filter and a spectrometer;

the blade clamp is made of shading materials and used for clamping the blade to be detected in the blade clamp;

the blade clamp is provided with an optical channel; the optical channel is used for enabling light rays to enter the blade clamp and reach the surface of the blade to be detected, and reflected light signals are transmitted to the optical fibers;

one end of the optical fiber is perpendicular to the blade to be measured, is arranged in the blade clamp and is used for acquiring an optical signal of light reflected by the blade to be measured through an optical channel;

the spectrometer is connected with the other end of the optical fiber to acquire the optical signal and is used for determining the irradiance of the blade to be measured according to the optical signal when no optical filter is arranged at the light ray inlet of the optical channel; when the optical filter is arranged at the light ray inlet of the optical channel, determining the fluorescence spectrum of the blade to be detected according to the optical signal;

the module that awaits measuring includes: a temperature sensor; the temperature sensor is used for measuring the temperature of the blade to be measured;

the main control processing module is used for receiving the measurement data of the blade to be measured and determining chlorophyll fluorescence parameters of the blade to be measured based on the measurement data of the blade to be measured;

wherein, the measured data of the blade to be measured includes: the irradiance of the blade to be measured, the fluorescence spectrum of the blade to be measured and the temperature of the blade to be measured.

2. The apparatus according to claim 1, wherein the optical channel comprises: a first optical channel and a second optical channel; the optical fiber includes: a first optical fiber and a second optical fiber;

the optical filter is arranged at the light ray inlet of the second optical channel;

the spectrometer is connected with the first optical fiber and the second optical fiber, a first optical signal is obtained through the first optical fiber, and irradiance of the blade to be measured is determined according to the first optical signal; and acquiring a second optical signal through the second optical fiber, and determining the fluorescence spectrum of the blade to be detected according to the second optical signal.

3. The method for detecting the chlorophyll fluorescence parameters of the plant leaves as claimed in claim 1, wherein said light-shielding material is black ABS plastic.

4. A method for detecting chlorophyll fluorescence parameters of plant leaves, which is implemented by the apparatus for detecting chlorophyll fluorescence parameters of plant leaves according to any one of claims 1 to 3, and comprises:

determining chlorophyll fluorescence parameters of the to-be-detected leaf based on the measurement data of the to-be-detected leaf;

the irradiance of the blade to be measured, the fluorescence spectrum of the blade to be measured and the temperature of the blade to be measured.

5. The method for detecting chlorophyll fluorescence parameters of plant leaves according to claim 4, wherein the determining chlorophyll fluorescence parameters of the leaves to be detected based on the measured data of the leaves to be detected specifically comprises:

determining the fluorescence spectrum reflectivity of the blade to be detected based on the irradiance of the blade to be detected and the fluorescence spectrum of the blade to be detected;

and determining chlorophyll fluorescence parameters of the blade to be detected based on the irradiance of the blade to be detected, the fluorescence spectrum reflectivity and the temperature of the blade to be detected.

6. The method for detecting the chlorophyll fluorescence parameters of the plant leaves as claimed in claim 5, wherein the determining the fluorescence spectrum reflectivity of the leaves to be detected based on the irradiance of the leaves to be detected and the fluorescence spectrum of the leaves to be detected specifically comprises:

determining the fluorescence spectrum reflectivity of the blade to be detected according to a fluorescence spectrum reflectivity calculation formula based on the irradiance of the blade to be detected and the fluorescence spectrum of the blade to be detected;

the fluorescence spectrum reflectivity calculation formula is as follows:

wherein R is the fluorescence spectrum reflectivity, L is the blade irradiance to be measured, B is the dark current, and I is the whiteboard irradiance.

7. The method for detecting the fluorescence parameters of the chlorophyll of the leaves of the plant according to claim 6, wherein before the step of determining the fluorescence spectrum reflectivity of the leaves to be detected according to a fluorescence spectrum reflectivity calculation formula based on the irradiance of the leaves to be detected and the fluorescence spectrum of the leaves to be detected, the method further comprises:

calibrating a white board based on the plant leaf chlorophyll fluorescence parameter detection device to obtain the white board irradiance;

and collecting the dark current when the plant leaf chlorophyll fluorescence parameter detection device does not clamp the leaf to be detected.

8. The method for detecting the chlorophyll fluorescence parameters of the plant leaves according to any one of claims 5 to 7, wherein the determining the chlorophyll fluorescence parameters of the leaves to be detected based on the irradiance of the leaves to be detected, the fluorescence spectrum reflectivity and the temperature of the leaves to be detected specifically comprises:

calculating heat dissipation energy of the blade to be detected based on the temperature of the blade to be detected, and determining corrected fluorescence intensity;

determining a compensated fluorescence spectral reflectance based on the fluorescence spectral reflectance and the corrected fluorescence intensity;

and determining the chlorophyll fluorescence parameters of the blade to be detected based on the compensated fluorescence spectrum reflectivity.

9. The method for detecting the chlorophyll fluorescence parameters of the plant leaves according to claim 8, wherein the determining the compensated fluorescence spectrum reflectivity based on the irradiance of the leaves to be detected, the fluorescence spectrum reflectivity and the corrected fluorescence intensity specifically comprises:

based on the irradiance of the blade to be detected, performing fluorescence inversion according to a Fraunhofer dark line method, and determining the inverted fluorescence intensity;

and determining the compensated fluorescence spectrum reflectivity based on the inverted fluorescence intensity and the fluorescence spectrum reflectivity.

10. The method for detecting chlorophyll fluorescence parameters of plant leaves according to claim 8, wherein the determining chlorophyll fluorescence parameters of the leaves to be detected based on the compensated fluorescence spectrum reflectivity specifically comprises:

determining a corrected fluorescence ratio index based on the compensated fluorescence spectrum reflectivity; the corrected fluorescence ratio index is used for expressing the ratio of the fluorescence reflectivity of the blade to be detected after different wave bands are compensated;

and determining the chlorophyll fluorescence parameters of the blade to be detected based on the corrected fluorescence ratio index.

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