CN115006581A - Material for wound protection and preparation method thereof - Google Patents
Material for wound protection and preparation method thereof Download PDFInfo
- Publication number
- CN115006581A CN115006581A CN202210624949.3A CN202210624949A CN115006581A CN 115006581 A CN115006581 A CN 115006581A CN 202210624949 A CN202210624949 A CN 202210624949A CN 115006581 A CN115006581 A CN 115006581A
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- Prior art keywords
- carrier
- virus
- wound
- preparing
- protecting
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- 239000000463 material Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title description 6
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 49
- 206010052428 Wound Diseases 0.000 claims abstract description 47
- 241000700605 Viruses Species 0.000 claims abstract description 37
- 239000000725 suspension Substances 0.000 claims abstract description 21
- 230000029663 wound healing Effects 0.000 claims abstract description 12
- 230000001737 promoting effect Effects 0.000 claims abstract description 7
- 238000004113 cell culture Methods 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 11
- 239000003480 eluent Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- 238000012423 maintenance Methods 0.000 claims description 6
- 239000008188 pellet Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 210000003501 vero cell Anatomy 0.000 claims description 6
- 230000000120 cytopathologic effect Effects 0.000 claims description 4
- 238000009966 trimming Methods 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 claims description 3
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 231100000645 Reed–Muench method Toxicity 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 235000009120 camo Nutrition 0.000 claims description 3
- 235000005607 chanvre indien Nutrition 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 239000011487 hemp Substances 0.000 claims description 3
- 238000002941 microtiter virus yield reduction assay Methods 0.000 claims description 3
- 231100000572 poisoning Toxicity 0.000 claims description 3
- 230000000607 poisoning effect Effects 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000004448 titration Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 2
- 238000009941 weaving Methods 0.000 claims description 2
- 230000035876 healing Effects 0.000 description 5
- 230000006378 damage Effects 0.000 description 3
- 206010063560 Excessive granulation tissue Diseases 0.000 description 2
- 210000001126 granulation tissue Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035752 proliferative phase Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/76—Viruses; Subviral particles; Bacteriophages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Materials Engineering (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Zoology (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a material for wound protection, which comprises a carrier, wherein a virus suspension for protecting a wound and promoting wound healing is dip-coated on the carrier. The wound protection material with the structure is attached to the wound by the carrier which is dipped and coated with the virus suspension for protecting the wound and promoting the wound healing, so that the wound healing can be promoted on the basis of avoiding the wound from being polluted by the outside.
Description
Technical Field
The invention relates to a wound protection technology, in particular to a material for wound protection and a preparation method thereof.
Background
Natural healing of wounds in the body is a complex process that starts from the time of injury. First, the body minimizes injury by delivering proteins and other clotting substances to the wound via blood flow. The blood clots to prevent blood loss while the cells engulf bacteria and debris at the wound site. Next, a healing phase called the proliferative phase, the body begins to repair itself and granulation tissue is deposited on the wound bed. The granulation tissue provides a base structure on the wound and cells can migrate from the perimeter of the wound to the interior to close the wound in a peri-wound manner. Eventually, scars form over time.
In most cases, the natural healing process is very effective. However, some wounds are affected by factors such as physical quality of patients, size and severity of wounds, and complicated infection, so that the healing speed is slow, and secondary damage caused by infection is always easy to be caused in the healing process, so that a material for wound protection and a preparation method thereof are urgently needed.
Disclosure of Invention
The invention aims to provide a material for wound protection, which is attached to a wound by a carrier which is dipped and coated with virus suspension for protecting the wound and promoting the wound healing, and can promote the wound healing on the basis of avoiding the wound from being polluted by the outside.
In order to achieve the above object, the present invention provides a material for wound protection, comprising a carrier on which a viral suspension for wound protection and wound healing promotion is dip-coated.
Preferably, the carrier is woven from primarily cotton, hemp and fiber.
Preferably, the strength of the carrier is not less than 300CN/tex, and the breaking elongation of the carrier is not less than 6%.
Preferably, the carrier has a ply twist of 10 twists/inch and the carrier has a ply twist direction of S twist.
The preparation method based on the material for wound protection comprises the following steps:
s1, preparing the pellet block,
trimming the carrier into a square block, cleaning the square block by using deionized water, and finally drying and sterilizing the square block to obtain a carrier block for later use;
s2, poisoning
S21, dividing the carrier blocks obtained by the processing of the step S1 into three types and eight types in a biological safety cabinet, and respectively placing the carrier blocks of each type into a twelve-hole cell culture plate;
s22, sucking 50 mul of virus suspension by a pipette, dripping the virus suspension on the surface of the pellet block, and standing at room temperature and 35% relative humidity;
s23, eluting the virus for 0h, 1h, 2h, 6h, 24h, 48h and 72h after the virus suspension is added;
s3 Virus titration experiment
S31, adding Vero cells into a ninety-six-hole cell culture plate in advance;
s32, continuously diluting the virus eluent by 10 times, adding the virus eluent into a ninety-six-hole cell culture plate to titrate the virus, observing and recording cytopathic effect on the fourth day of titration, and calculating TCID50 by a Reed-Muench method.
Preferably, in step S1: trimming the carrier into a 1x1cm square block;
sterilizing with pressure steam equipment.
Preferably, in step S22: the virus titer of the virus suspension is 106TCID 50/ml;
the temperature range of room temperature is 25-27 ℃.
Preferably, the step of eluting the virus in step S23 specifically includes:
s231, adding 500 mu l of cell maintenance liquid into a twelve-hole cell culture plate;
s232, repeatedly blowing and beating the surface of the cell maintenance liquid, and then collecting eluent;
s233, three replicates were made at each time point in each sample.
Preferably, the number of Vero cells in step S31 is 2X 104 cells/well.
Therefore, the wound protection material with the structure is attached to the wound by the carrier which is dipped and coated with the virus suspension for protecting the wound and promoting the wound healing, and the wound healing can be promoted on the basis of avoiding the wound from being polluted by the outside.
The technical solution of the present invention is further described in detail by the following examples.
Detailed Description
The present invention will be further described below, and it should be noted that the present embodiment is based on the technical solution, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the present embodiment.
The structure of the invention comprises a carrier, and the carrier is dipped and coated with virus suspension for protecting wounds and promoting wound healing. The carrier is mainly formed by weaving cotton, hemp and fibers. The strength of the carrier is not less than 300CN/tex, and the breaking elongation of the carrier is not less than 6%. The ply twist of the carrier is 10 twists/inch, the ply twist direction of the carrier is S twist, the carrier material is easy to obtain, the manufacturing cost is low, the production cost is reduced, the carrier can be repeatedly used after being soaked in the virus suspension again after being fully washed, and the carrier is more energy-saving and environment-friendly.
The preparation method based on the material for wound protection comprises the following steps:
s1, preparing the pellet block,
trimming the carrier into square blocks, cleaning the square blocks by using deionized water, and finally drying and sterilizing the square blocks to obtain carrier blocks for later use;
preferably, in step S1: the carrier is trimmed into a 1x1cm square block shape;
sterilizing with pressure steam equipment.
S2, poisoning
S21, dividing the carrier blocks obtained by the processing of the step S1 into three types and eight types in a biological safety cabinet, and respectively placing the carrier blocks of each type into a twelve-hole cell culture plate;
s22, sucking 50 mul of virus suspension by a pipette, dripping the virus suspension on the surface of the pellet block, and standing at room temperature and 35% relative humidity;
preferably, in step S22: the virus titer of the virus suspension is 106TCID 50/ml;
s23, eluting the virus for 0h, 1h, 2h, 6h, 24h, 48h and 72h after the virus suspension is added;
the temperature range of room temperature is 25-27 ℃.
Preferably, the step of eluting the virus in step S23 specifically includes:
s231, adding 500 mu l of cell maintenance liquid into a twelve-hole cell culture plate;
s232, repeatedly blowing and beating the surface of the cell maintenance liquid, and then collecting eluent;
s233, three replicates were made at each time point in each sample.
S3 Virus titration experiment
S31, adding Vero cells into a ninety-six-hole cell culture plate in advance;
preferably, the number of Vero cells in step S31 is 2X 104 cells/well.
S32, virus eluent is diluted continuously by 10 times, added into a ninety-six-hole cell culture plate to be titrated with virus, cytopathic effect (CPE) is observed and recorded on the fourth day of titration, and TCID50 is calculated by a Reed-Muench method.
Therefore, the wound protection material with the structure is attached to the wound by the carrier which is dipped and coated with the virus suspension for protecting the wound and promoting the wound healing, and the wound healing can be promoted on the basis of avoiding the wound from being polluted by the outside.
Finally, it should be noted that: the above embodiments are only intended to illustrate the technical solution of the present invention and not to limit the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.
Claims (9)
1. A material for wound protection, characterized in that: comprises a carrier, wherein the carrier is dipped and coated with virus suspension for protecting wounds and promoting wound healing.
2. A material for wound protection according to claim 1, wherein: the carrier is mainly formed by weaving cotton, hemp and fibers.
3. A material for wound protection according to claim 2, wherein: the strength of the carrier is not less than 300CN/tex, and the breaking elongation of the carrier is not less than 6%.
4. A material for wound protection according to claim 2, wherein: the carrier has a ply twist of 10 twists/inch and the carrier has a ply twist direction of S twist.
5. A method of preparing a material for wound protection according to any one of claims 1 to 4, wherein: the method comprises the following steps:
s1, preparing the pellet block,
trimming the carrier into square blocks, cleaning the square blocks by using deionized water, and finally drying and sterilizing the square blocks to obtain carrier blocks for later use;
s2, poisoning
S21, dividing the carrier blocks obtained by the processing of the step S1 into three types and eight types in a biological safety cabinet, and respectively placing the carrier blocks of each type into a twelve-hole cell culture plate;
s22, sucking 50 mul of virus suspension by a pipette, dripping the virus suspension on the surface of the pellet block, and standing at room temperature and 35% relative humidity;
s23, eluting the virus for 0h, 1h, 2h, 6h, 24h, 48h and 72h after the virus suspension is added;
s3 Virus titration experiment
S31, adding Vero cells into a ninety-six-hole cell culture plate in advance;
s32, continuously diluting the virus eluent by 10 times, adding the virus eluent into a ninety-six-hole cell culture plate to titrate the virus, observing and recording cytopathic effect on the fourth day of titration, and calculating TCID50 by a Reed-Muench method.
6. The method for preparing a material for protecting wound according to claim 5, wherein: in step S1: the carrier is trimmed into a 1x1cm square block shape;
sterilizing with pressure steam equipment.
7. The method for preparing a material for protecting wound according to claim 5, wherein: in step S22: the virus titer of the virus suspension is 106TCID 50/ml;
the temperature range of room temperature is 25-27 ℃.
8. The method for preparing a material for protecting wound according to claim 5, wherein: the step of eluting the virus in step S23 specifically includes:
s231, adding 500 mu l of cell maintenance liquid into a twelve-hole cell culture plate;
s232, repeatedly blowing and beating the surface of the cell maintenance liquid, and then collecting eluent;
s233, three replicates were made at each time point in each sample.
9. The method for preparing a material for protecting wound according to claim 5, wherein: the number of Vero cells in step S31 was 2X 104 cells/well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210624949.3A CN115006581A (en) | 2022-06-02 | 2022-06-02 | Material for wound protection and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210624949.3A CN115006581A (en) | 2022-06-02 | 2022-06-02 | Material for wound protection and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115006581A true CN115006581A (en) | 2022-09-06 |
Family
ID=83072269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210624949.3A Pending CN115006581A (en) | 2022-06-02 | 2022-06-02 | Material for wound protection and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115006581A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6521265B1 (en) * | 2000-02-09 | 2003-02-18 | Biolife, L.L.C. | Method for applying a blood clotting agent |
| CN103191462A (en) * | 2012-01-05 | 2013-07-10 | 王伟 | Medical bleeding-stopping and disinfecting biological dressing |
| CN103747792A (en) * | 2011-06-23 | 2014-04-23 | 固定噬菌体有限公司 | Delivery of viral agents |
| CN104768567A (en) * | 2012-05-18 | 2015-07-08 | 奥塔哥创业有限公司 | Combination treatments and compositions for wound healing |
| CN105263529A (en) * | 2013-04-12 | 2016-01-20 | 芒果基因制药公司 | Therapeutic virus particles for promoting scaffold biofunction and wound healing in vertebrate individuals |
| CN105435295A (en) * | 2015-12-15 | 2016-03-30 | 哈尔滨工业大学 | Preparation method of RGD-M13 bacteriophage/oxidized regenerated cellulose composite hemostatic material |
| CN111643724A (en) * | 2020-07-01 | 2020-09-11 | 南京大学 | A kind of preparation method and application of hydrogel wound dressing loaded with bacteriophage and acidic fibroblast growth factor |
-
2022
- 2022-06-02 CN CN202210624949.3A patent/CN115006581A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6521265B1 (en) * | 2000-02-09 | 2003-02-18 | Biolife, L.L.C. | Method for applying a blood clotting agent |
| CN103747792A (en) * | 2011-06-23 | 2014-04-23 | 固定噬菌体有限公司 | Delivery of viral agents |
| CN103191462A (en) * | 2012-01-05 | 2013-07-10 | 王伟 | Medical bleeding-stopping and disinfecting biological dressing |
| CN104768567A (en) * | 2012-05-18 | 2015-07-08 | 奥塔哥创业有限公司 | Combination treatments and compositions for wound healing |
| CN105263529A (en) * | 2013-04-12 | 2016-01-20 | 芒果基因制药公司 | Therapeutic virus particles for promoting scaffold biofunction and wound healing in vertebrate individuals |
| CN105435295A (en) * | 2015-12-15 | 2016-03-30 | 哈尔滨工业大学 | Preparation method of RGD-M13 bacteriophage/oxidized regenerated cellulose composite hemostatic material |
| CN111643724A (en) * | 2020-07-01 | 2020-09-11 | 南京大学 | A kind of preparation method and application of hydrogel wound dressing loaded with bacteriophage and acidic fibroblast growth factor |
Non-Patent Citations (1)
| Title |
|---|
| 刘静杰: "《携带编码人血管内皮生长因子165的重组腺病毒能促进伤口愈合》", 《中国危重病急救医学》, vol. 7, pages 433 - 110 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220906 |
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