CN114642999A - Reaction vessel mixing device, cleaning measuring device and in vitro diagnostic analyzer - Google Patents

Abstract

The invention discloses a reaction vessel blending device and application thereof, comprising: the driving mechanism drives the eccentric wheel to rotate; the mounting shell is provided with a limiting frame, and one end of the oscillating seat is provided with a central hole for accommodating the eccentric wheel; the other end of the vibration seat penetrates through the limiting frame and is provided with a clamping groove, the reaction container is inserted into the clamping groove, a gap is reserved between the reaction container and the side wall of the clamping groove, the eccentric wheel can drive the vibration seat to reciprocate in the limiting frame in the center hole, and when the vibration seat reciprocates, the side walls on two sides of the clamping groove can impact the reaction container.

Description

Reaction vessel mixing device, cleaning measuring device and in vitro diagnostic analyzer

technical field

The invention relates to the in vitro diagnostic industry, in particular to a reaction vessel mixing device, a cleaning and measuring device and an in vitro diagnostic analyzer.

Background technique

In the sample analysis instrument, a mixing device is often required to realize the mixing of the cuvette at each operating position. The existing sample reagent mixing is mostly contact mixing, such as the stirring rod method. The contact mixing method may cause cross-contamination. risk, or set up an independent mixing mechanism. After the mixing mechanism is mixed, it will be transferred to the next operating position by the robot, and the additional mixing mechanism will take up space.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a reaction container mixing device with ingenious structure, compactness and good vibration effect, which is suitable for mixing reaction containers arranged in the card slot in various in vitro diagnostic instruments.

The technical scheme adopted in the present invention is as follows: a reaction vessel mixing device, comprising:

Eccentric wheel;

a driving mechanism, the driving mechanism drives the eccentric wheel to rotate;

an installation shell, the installation shell and the bottom plate-shaped limiting frame to be installed with the mixing device;

An oscillation seat, one end of the oscillation seat has a center hole for accommodating the eccentric wheel; the other end of the oscillation seat passes through the limit frame and has a card slot, and the reaction container is inserted into the card slot, and is connected with the card slot. There is a gap between the side walls of the slot. The rotation of the eccentric wheel in the central hole can drive the oscillation seat to reciprocate in the limit frame. When the oscillation seat reciprocates, the two sides of the slot The side walls can strike the reaction vessel.

Further, the mixing device further includes an optocoupler sheet and a detection optocoupler, the detection optocoupler is connected to the end wall of the oscillation seat with a central hole, and the detection optocoupler is based on whether the optocoupler sheet is Reset to the detection photocoupler to detect whether the vibration seat hits the reaction vessel.

Further, the reaction vessel is a reaction cup.

The present invention also provides a cleaning and measuring device, including the above reactor mixing device.

Further, the cleaning and cleaning measuring device includes:

a transfer plate, used for transferring the cuvette to a corresponding operation position, and the operation position is distributed in the circumferential direction of the transfer plate;

The reaction vessel mixing device is used for mixing the reaction cup in the operation position; the lower part of the reaction cup in the operation position is inserted into the card slot and has a gap with the side wall of the card slot.

The reaction vessel mixing device is suitable for mixing reaction cups on any operating position.

The present invention also provides an in vitro diagnostic analyzer, comprising the above-mentioned mixing device or the above-mentioned cleaning and measuring device

Beneficial effects of the present invention: The reaction vessel mixing device of the present invention has an ingenious structure, is compact, and has a good vibration effect, and is suitable for mixing reaction vessels arranged in a card slot in various in vitro diagnostic instruments.

Description of drawings

In order to illustrate the technical solutions of the present invention more clearly, the accompanying drawings that need to be used in the embodiments will be briefly introduced below. Other drawings can also be obtained from these drawings.

Fig. 1 is the structure schematic diagram of mixing mechanism;

Fig. 2 is a cross-sectional view of a mixing mechanism;

Figure 3 is the bottom view of the mixing mechanism;

Fig. 4 is a schematic diagram of a fully shielded structure of the pick-and-place opening of the cleaning and measuring device cuvette;

Fig. 5 is a schematic diagram of the fully open structure of the cuvette taking and placing opening of the cleaning measuring device;

6 is a sectional view of the cleaning measuring device;

7 is a schematic structural diagram of a shading device;

1- Bottom plate; 2- Outer shell; 3- Transfer plate; 4- Detection mechanism; 5- Reaction cup pick and place port; 6- Shading device; 7- Liquid injection mechanism; 8- Liquid discharge mechanism; 9- Mixing device ; 10- reaction cup; 11- acid addition mechanism; 12- alkali addition mechanism; 101- lift motor; 102- screw rod; 103- lift plate; 104- guide mechanism; 201- base; 202- top cover; 203- drive Motor; 204-rolling bearing; 61-shading plate; 62-drive unit; 63-code disc; 64-detection photocoupler one; 901-eccentric wheel; 902-drive mechanism; 903-installation shell; 904-oscillating seat; 905- Mounting hole; 906-card slot; 907-optical coupler; 908-detection coupler II.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the corresponding drawings. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. The technical solutions provided by the embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

Referring to FIGS. 1-3 , a reaction vessel mixing device 9 includes: an eccentric wheel 901 , a driving mechanism 902 , a mounting shell 903 and a vibration seat 904 .

The function of the driving mechanism 902 is to drive the eccentric wheel 901 to rotate, and the specific structure may not be limited.

The mounting shell 903 is composed of an upper shell and a lower shell, forming a limit frame. The specific structure is not limited. At the same time, it is convenient to install the mixing mechanism.

One end of the vibration seat 904 has a mounting hole 905 for accommodating the eccentric wheel 901 ; the other end of the vibration seat 904 passes through the limit frame and has a card slot 906 , and the lower part of the reaction vessel is inserted into the card slot 906 and is connected with the side wall of the card slot 906 There is a gap therebetween, and the rotation of the eccentric wheel 901 in the installation hole 905 can drive the vibration seat 904 to reciprocate in the limit frame 906 . The specific shape of the above reaction vessel is not limited, and may be a test tube or a reaction cup.

In the embodiment provided by the present invention, the vibration seat 904 passes through the limiting frame, the end with the mounting hole 905 is located in the mounting shell 906, and the other end with the slot 906 protrudes out of the mounting shell 903. The shape of the middle section of the vibration seat is The size is the same as the size of the limit frame, the vibration seat 904 is limited up, down, front and rear, and only the vibration seat 904 is allowed to reciprocate left and right.

Further, in order to realize the reciprocating motion of the oscillating seat 904 more stably, in this embodiment, the mounting hole 905 is arranged in the center of the end of the oscillating seat 904, the shape of the mounting hole 905 is a waist shape, and the width of the waist-shaped mounting hole is the same as that of the eccentric wheel. The length of the diameter of 901 is the same, and the width of the card slot is not more than 2 times the distance from the rotation point of the eccentric wheel to the center point.

During operation, when the eccentric wheel 901 drives the vibration seat 904 to move to the right in the limit frame 906, the left side wall of the card slot 906 can hit the cuvette. When the eccentric wheel drives the vibration seat 904 to move to the left in the limit frame 906 , the right side wall of the card slot 906 can hit the cuvette, so that the liquid in the cuvette forms a turbulent flow and promotes the reaction of the liquid in the cuvette.

The above-mentioned reaction vessel mixing device also includes an optocoupler 907 and a detection optocoupler 2 908. The detection optocoupler 2 908 is connected to the end wall of the oscillation seat 904 with the mounting hole, and the detection optocoupler 2 908 is reset to the position according to whether the optocoupler 907 is reset. The second 908 of the optical coupler is detected to detect whether the vibration seat 904 hits the cuvette.

Example 2

A cleaning and measuring device, as shown in Figures 4-7, includes a magnetic bead cleaning and separation device. The magnetic bead cleaning and separation device is an existing structure, including a bottom plate 1, an outer casing 2; a transfer plate 3, and a magnetic part, note Liquid mechanism 7 and drain mechanism 8.

An outer casing 2 is mounted on the bottom plate 1, and the outer casing 2 is made of opaque material. In this embodiment, as shown in FIG. 3, the outer casing includes a base 201 and a cover arranged on the bottom plate The top cover 202 on the base, the upper part of the base has a hollow cavity, the transfer plate 3 is arranged in the hollow cavity of the base 201, one end of the rotating shaft passes through the base and is connected to the gear plate 203, and the gear plate 203 is connected to the motor through a belt, The other end is connected to the transfer plate 3 through the base 201, the rotating shaft is connected to the base 201 through the rolling bearing 204, and the transfer plate has a plurality of holes for placing the reaction cups; when the motor rotates, the base is fixed, and the motor rotates to drive the transfer plate 3 to place the reaction cups. Transfer to the appropriate operating location.

The function of the transfer plate 3 transfers the cuvette to the corresponding operating position, and the operating position is distributed in the circumferential direction of the transfer disk, including at least the cuvette pick-and-place position and the cleaning position; the cleaning position includes the liquid injection level and the liquid discharge level.

The magnetic piece is arranged on the side of the transfer path of the transfer plate 3; the specific magnetic piece is arranged on the inside or outside of the transfer path of the transfer plate according to the cleaning effect, so as to realize that the magnetic beads of the cuvette are respectively gathered to the magnetic piece at the corresponding operating position one side of the cuvette to fully disperse and effectively clean the magnetic beads during the transfer of the cuvette.

The liquid injection mechanism 7 is correspondingly arranged at the liquid injection position, and is used for injecting cleaning liquid into the reaction cup at the liquid injection position;

The liquid draining mechanism 8 is correspondingly disposed at the liquid draining level, and is used for draining the liquid in the reaction cup at the liquid draining level.

Specifically, the cleaning position includes three liquid injection positions and three liquid discharge positions, and the three liquid injection positions and three liquid discharge positions are located between the pick-and-place position of the reaction cup and the acid addition position and along the circumference of the transfer plate. Alternately distributed, the liquid injection mechanism 7 includes three liquid injection needles, and the three liquid injection needles are respectively set corresponding to the three liquid injection levels. The liquid discharge mechanism 8 includes three liquid discharge needles, three liquid discharge needles. They are respectively set corresponding to the discharge positions.

The liquid injection mechanism 7 is fixed at the liquid injection level of the top cover, and the liquid discharge mechanism 8 is driven vertically up and down by the lifting mechanism to drain the liquid in the reaction cup at the liquid discharge level; the lifting mechanism is installed above the transfer plate, including lifting and lowering The motor 101, the screw rod 102 and the lifting plate 103, the discharge needles are all fixed on the lifting plate, the upper end of the screw rod 102 is connected with the lifting motor 101, and the lifting motor 101 drives the screw rod 102 to rotate, thereby realizing the liquid injection needle vertical lifting movement. Further, the lifting mechanism 10 also includes two guide mechanisms 104 symmetrically arranged, so as to realize the lifting and lowering of the lifting plate more stably. The guide mechanism 10 includes a guide shaft and a guide bushing. The lower part of the guide shaft is fixed with the top cover, and the upper part is It is fixed with the lifting plate through the guide bushing.

In this embodiment, the cleaning, separation and measurement of magnetic beads are integrated, and specifically, it further includes an acid adding mechanism 11 , an alkali adding mechanism 12 , a detection mechanism 4 and the above-mentioned reactor mixing device 9 .

The acid addition mechanism 11 is correspondingly arranged at the acid addition position, and the specific structure is not limited, as long as the acid excitation liquid can be injected into the acid addition position reaction cup, and the acid addition mechanism 11 is fixed at the top cover acid addition position .

The alkali addition mechanism 12 is correspondingly arranged at the alkali addition position, and the specific structure is not limited, as long as the alkali excitation liquid can be injected into the alkali addition position reaction cup, and the alkali addition mechanism 12 is fixed on the top cover alkali addition position place.

The detection mechanism 4, corresponding to the photometric position, can be an existing detection mechanism for detecting the luminous intensity of the cuvette in the photometric position. The top wall of the outer casing 2 corresponds to the cuvette. The placement position is also provided with a cuvette pick-and-place port 5; a photometric through hole is opened on the side wall of the outer casing, and the detection mechanism 4 is installed at the photometric through hole and passes through the photometric through hole. The hole detects the luminous intensity of the cuvette in the photometric position.

The above-mentioned reactor mixing device 9 is correspondingly arranged at the acid addition position, and is used for mixing the reaction cup in the acid addition position. Specifically, the lower part of the reaction cup 10 in the acid addition position is inserted into the card slot. 906, and there is a gap with the side wall of the card slot 906.

Working process: the cuvette 10 is placed in the cuvette pick-and-place position of the transfer plate 3 from the cuvette pick-and-place port 5, and as the transfer disk 3 rotates to the liquid injection level, the liquid injection needle injects the cleaning liquid into the cuvette to realize Cleaning of the reaction cup; the reaction cup 10 continues to rotate with the transfer plate 3 to the liquid discharge level, and the liquid discharge needle moves vertically downward into the reaction cup to suck away the liquid in the reaction cup; one pumping and suction is one cleaning ; Repeat the liquid injection and suction three times to achieve three cleanings; after the three cleanings, the reaction cup rotates with the transfer plate 3 to add acid, and the acid excitation liquid is injected into the reaction cup, at this time, the mixing mechanism of the reactor will gather in the reaction cup one. The magnetic beads on the side are scattered to make them fully contact and react with the acidic excitation solution. The cuvette rotates to the alkali-adding position with the transfer plate 3, and then the alkaline excitation solution is injected into the cuvette, and the detection mechanism starts to measure light.

In order to measure more accurately, form a completely closed whole, avoid external light from entering the metering position to the greatest extent, and ensure the accuracy of the measurement results.

In another embodiment provided by the present invention, the cleaning measurement device further includes:

The shading device 6 is correspondingly arranged at the opening of the cuvette, which can completely cover or open the cuvette. bit position.

Specifically, the shading device 6 includes: a shading sheet 61 , a driving unit 62 , a code wheel 63 and a detection optical coupler 1 64 .

The shading sheet 61 rotates to completely cover or open the access opening of the reaction cup; the specific shape of the shading sheet 61 is not limited, and can be square, triangular or fan-shaped. In this embodiment, as shown in FIG. The shape is fan.

The driving unit 62 drives the light-shielding sheet 61 to rotate. The specific structure of the driving unit 62 is not limited, as long as it can drive the light-shielding sheet 61 to rotate forward and reverse, it can be an existing stepping motor.

The code disc 63 is driven by the driving unit 62 and rotates synchronously with the light shielding sheet 61. A gap 65 is provided on the outer edge of the code disc; the code disc 63 and the light shielding sheet 61 are both fixed on the stepping motor shaft superior.

The detection optical coupler 1 64 is arranged on the outer edge of the code disc 63 , and the detection optical coupler 1 64 detects the rotation angle of the code disc 63 according to whether the code disc is reset to one of the detection optical couplers. That is, to detect the rotation angle of the stepping motor, when the detection optocoupler 1 64 is blocked by the sidewall of the gap 65, it means that the code disc is successfully reset, and when the detection optocoupler is blocked by the sidewall of the gap , the shading sheet can be in the state of completely covering or opening the access opening of the reaction cup; in this embodiment, when the detection optocoupler 1 64 is blocked by the sidewall of the notch 65, it indicates that the code disc Successful reset, at this time, the light shielding sheet 61 completely opens the cuvette taking and placing port 5; when measurement is required, in order to form a completely closed measurement environment, the stepping motor rotates a preset angle, and the light shielding sheet 61 completely shields the The cuvette pick-and-place port 5 is described. After the measurement is completed, the stepping motor reverses the same angle, and the light shielding sheet 61 completely opens the cuvette-cup pick-and-place port 5 .

Example 3

An in-vitro diagnostic analyzer, comprising the above-mentioned measuring and cleaning device.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims (6)

1. The utility model provides a reaction vessel mixing device which characterized in that includes:

an eccentric wheel;

the driving mechanism drives the eccentric wheel to rotate;

the mounting shell is provided with a limiting frame;

the oscillating seat is provided with a central hole for accommodating the eccentric wheel at one end; the other end of the vibration seat penetrates through the limiting frame and is provided with a clamping groove, a reaction container is inserted into the clamping groove, a gap is reserved between the side walls of the clamping groove, the eccentric wheel can drive the vibration seat to rotate in the central hole to reciprocate in the limiting frame, and when the vibration seat reciprocates, the side walls on the two sides of the clamping groove can impact the reaction container.

2. The reaction vessel blending device of claim 1, wherein the blending device further comprises a light coupling piece and a detection optical coupler, the detection light coupler is coupled to the end wall of the oscillating base with the central hole, and the detection optical coupler is used for detecting whether the light coupling piece resets to the detection optical coupler to detect whether the oscillating base impacts the reaction vessel.

3. The apparatus of claim 1, wherein the reaction vessel is a reaction cup.

4. A cleaning and measuring device, comprising: the apparatus of any one of claims 1 to 3 for homogenizing a reactor.

5. The cleaning and measuring device of claim 4, comprising:

the transfer disc is used for transferring the reaction cups to corresponding operation positions, and the operation positions are distributed on the circumference of the transfer disc;

the reaction container uniformly mixing device is used for uniformly mixing the reaction containers in the operation position; the lower part of the reaction cup at the operation position is inserted into the clamping groove, and a gap is reserved between the lower part of the reaction cup and the side wall of the clamping groove.

6. An in vitro diagnostic analyzer, comprising the mixing device of any one of claims 1 to 3 or the cleaning and measuring device of claim 4.

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