CN114518461A - Sample analyzer and sample analyzing method - Google Patents

Abstract

A sample analyzer and sample analysis method, the sample analyzer includes the sample and dispenses the organization, reagent and dispenses the organization, reaction mechanism, measuring mechanism, ultrasonic apparatus and control device separately, the sample dispenses the organization and is used for absorbing the sample and discharging the sample to holding the cup; the reagent dispensing mechanism is used for sucking the reagent and discharging the reagent into the containing cup; the reaction mechanism is used for providing an incubation place for the reaction liquid in the containing cup, and the reaction liquid is formed by mixing a sample and a reagent; the measuring mechanism is used for measuring the reaction liquid; the ultrasonic device is used for generating ultrasonic vibration to form ultrasonic waves; the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample in the containing cup. Because the sample analyzer is provided with the ultrasonic device, the ultrasonic device is used for transmitting ultrasonic waves to the sample, and can uniformly disperse all components in the sample, so that the subsequent sample can fully react with the reagent, and the detection accuracy is further improved.

Description

Sample analyzer and sample analysis method

technical field

The invention relates to in vitro detection equipment, in particular to a sample analyzer and a sample analysis method.

Background technique

In vitro diagnosis refers to products and services that obtain clinical diagnostic information by testing human samples, such as blood, urine, etc., outside the human body, and then judge diseases or body functions. Because in vitro diagnostic methods can quickly and accurately diagnose diseases in the early stage, they are playing an increasingly important role in clinical medicine and related medical research.

In the process of detecting human samples, it is usually necessary to mix the samples to be tested and the corresponding reagents to form a reaction solution, and then use a mixing device to mix the reaction solution, so that the samples and the reagents can fully react. However, the initial test sample itself contains heterogeneous substances, and the introduction of the heterogeneous substances in the sample into the reaction system will easily affect the accuracy of the final test results.

SUMMARY OF THE INVENTION

In one embodiment, a sample analyzer is provided, comprising:

A sample dispensing mechanism for drawing the sample and discharging the sample into the holding cup;

The reagent dispensing mechanism is used to draw the reagent and discharge the reagent into the holding cup;

The reaction mechanism is used to provide an incubation place for the reaction solution in the holding cup, and the reaction solution is formed by mixing the sample and the reagent;

The measuring mechanism is used to measure the reaction solution;

an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and

A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample in the holding cup.

In one embodiment, the controller is configured to acquire test item parameters, and according to the test item parameters, perform an ultrasonic mixing operation on the sample by matching an ultrasonic mode from a plurality of preset ultrasonic modes.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In one embodiment, the sample dispensing mechanism includes a sampling needle, the ultrasonic device includes an ultrasonic transducer, the ultrasonic transducer is connected to the sampling needle, and the sampling needle is used to convert the ultrasonic The ultrasonic vibration generated by the transducer is transmitted into the sample.

In an embodiment, the ultrasonic device includes an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, the transmission member The first end is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the accommodating cup; the moving device is connected with the ultrasonic transducer, and the moving device is used to drive the The ultrasonic transducer and the transmission member move relative to the accommodating cup, and the second end of the transmission member can be inserted into the sample in the accommodating cup, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the accommodating cup in the sample.

In one embodiment, the ultrasonic device includes an ultrasonic transducer and a transmission member, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, and the first end of the transmission member is The end is connected with the ultrasonic transducer; the second end of the transmission member is used to abut on the outer wall of the accommodating cup, and the part where the outer wall of the accommodating cup and the transmission member is in contact is the part surrounding the sample, so that the The ultrasonic vibration generated by the ultrasonic transducer is transmitted to the sample in the holding cup.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases in steps from the first end to the second end.

In one embodiment, a sample analyzer is provided, comprising:

a sample carrying mechanism for carrying a sample container containing a sample;

a sample dispensing mechanism for sucking the sample in the sample container on the sample carrying mechanism and discharging the sample into the holding cup;

The reagent dispensing mechanism is used to draw the reagent and discharge the reagent into the holding cup;

The reaction mechanism is used to provide an incubation place for the reaction solution in the holding cup, and the reaction solution is formed by mixing the sample and the reagent;

The measuring mechanism is used to measure the reaction solution;

an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and

A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample in the sample container.

In one embodiment, the controller is configured to acquire test item parameters, and according to the test item parameters, perform an ultrasonic mixing operation on the sample by matching an ultrasonic mode from a plurality of preset ultrasonic modes.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In one embodiment, the sample dispensing mechanism includes a sampling needle, the ultrasonic device includes an ultrasonic transducer, the ultrasonic transducer is connected to the sampling needle, and the sampling needle is used to convert the ultrasonic The ultrasonic vibration generated by the transducer is transmitted into the sample.

In an embodiment, the ultrasonic device includes an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, the transmission member The first end is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the sample container; the moving device is connected with the ultrasonic transducer, and the moving device is used to drive the The ultrasonic transducer and the transmission member move relative to the sample container, and the second end of the transmission member can be inserted into the sample in the sample container, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the sample container in the sample.

In one embodiment, the ultrasonic device includes an ultrasonic transducer and a transmission member, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, and the first end of the transmission member is The end is connected with the ultrasonic transducer; the second end of the transmission member is used to abut against the outer wall of the sample container, and the part of the outer wall of the sample container in contact with the transmission member is the part surrounding the sample, so that the The ultrasonic vibration generated by the ultrasonic transducer is transmitted to the sample in the sample container.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases in steps from the first end to the second end.

In one embodiment, a sample analyzer is provided, comprising:

a dispensing mechanism for drawing the sample and discharging the sample into the holding cup, the dispensing mechanism is also used for drawing the pretreatment liquid and discharging the pretreatment liquid into the holding cup containing the sample;

an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and

A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample injected with the pretreatment liquid.

In one embodiment, the pretreatment solution includes at least one of a diluent and a pretreatment reagent.

In one embodiment, the dispensing mechanism includes a sample dispensing mechanism and a reagent dispensing mechanism,

The sample dispensing mechanism is used for sucking the sample and discharging the sample into the accommodating cup, and the sample dispensing mechanism is also used for sucking the diluent and discharging the diluent into the accommodating cup with the sample;

The reagent dispensing mechanism is used to draw the pretreatment reagent and discharge the pretreatment reagent into the holding cup with the sample.

In one embodiment, the controller is used to obtain test item parameters, and according to the test item parameters, match an ultrasonic mode from a plurality of preset ultrasonic modes to perform an ultrasonic mixing operation on the sample injected with the pretreatment reagent. .

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In one embodiment, the sample dispensing mechanism includes a sampling needle, the ultrasonic device includes an ultrasonic transducer, the ultrasonic transducer is connected to the sampling needle, and the sampling needle is used to convert the ultrasonic The ultrasonic vibration generated by the energy generator is transmitted to the sample injected with the pretreatment liquid.

In an embodiment, the ultrasonic device includes an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, the transmission member The first end is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the sample container; the moving device is connected with the ultrasonic transducer, and the moving device is used to drive the The ultrasonic transducer and the transmission member move relative to the sample container, and the second end of the transmission member can be inserted into the sample injected with the pretreatment liquid in the sample container, so as to transmit the ultrasonic waves generated by the ultrasonic transducer. The vibrations are transmitted to the sample infused with the pretreatment solution in the sample container.

In one embodiment, the ultrasonic device includes an ultrasonic transducer and a transmission member, the ultrasonic transducer is used to form ultrasonic vibration, the transmission member has a first end and a second end, and the first end of the transmission member is The end is connected with the ultrasonic transducer; the second end of the transmission member is used to abut on the outer wall of the sample container, and the part of the outer wall of the sample container in contact with the transmission member is a part surrounding the sample injected with the pretreatment liquid. part, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the sample injected with the pretreatment liquid in the sample container.

In one embodiment, the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases in steps from the first end to the second end.

In one embodiment, a sample analysis method is provided, comprising the following steps:

The sample dispensing mechanism injects the sample into the holding cup;

The ultrasonic device emits ultrasonic waves into the sample of the holding cup;

The reagent dispensing mechanism injects the reagent into the holding cup, and the reagent and the ultrasonicated sample form a reaction solution;

The reaction solution is incubated in the reaction mechanism;

The measuring mechanism performs optical measurement on the reaction solution.

In one embodiment, the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps:

Get test project parameters;

According to the test item parameters, an ultrasonic mode is matched from a plurality of preset ultrasonic modes to perform an ultrasonic mixing operation on the sample.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

In an embodiment, between the step of injecting the sample into the holding cup by the sample dispensing mechanism and the step of transmitting ultrasonic waves into the sample in the holding cup, the step further includes the following steps:

At least one of a diluent and a pretreatment reagent is injected into the holding cup with the sample.

In one embodiment, a sample analysis method is provided, comprising the following steps:

The ultrasonic device emits ultrasonic waves into the sample in the sample container on the sample carrying mechanism;

The sample dispensing mechanism sucks the sonicated sample in the sample container on the sample carrying mechanism, and injects the sonicated sample into the holding cup;

The reagent dispensing mechanism injects the reagent into the holding cup;

The reaction solution is incubated in the reaction mechanism, and the reaction solution is formed by mixing the reagents and the sonicated sample;

The measuring mechanism performs optical measurement on the reaction solution.

In one embodiment, the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps:

Get test project parameters;

According to the test item parameters, an ultrasonic mode is matched from a plurality of preset ultrasonic modes to perform an ultrasonic mixing operation on the sample.

In one embodiment, the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively.

According to the sample analyzer and the sample analysis method of the above-mentioned embodiments, since the sample analyzer is provided with an ultrasonic device, the ultrasonic device is used to transmit ultrasonic waves into the sample, and the components in the sample can be uniformly dispersed, so that the subsequent sample can fully react with the reagent, Thus, the detection accuracy is improved.

Description of drawings

1 is a schematic structural diagram of an immunoluminescence analyzer in an embodiment;

Fig. 2 is a structural block diagram of the control part of the immunoluminescence analyzer in an embodiment;

3 is a schematic structural diagram of a vortex mixing device in an embodiment;

4 is a schematic structural diagram of a contact ultrasonic device in an embodiment;

FIG. 5 is a structural view of a transmission member in an embodiment;

FIG. 6 is a structural view of a transmission member in an embodiment;

7 is a schematic diagram of a mobile device in an embodiment;

8 is a schematic diagram of an ultrasonic device in an embodiment;

9 is a schematic structural diagram of a non-contact ultrasonic device in an embodiment;

10 is a schematic structural diagram of a non-contact ultrasonic device in an embodiment;

Figure 11 is a side view of a gripping device in one embodiment;

FIG. 12 is a top view of a gripping device in one embodiment;

13 is a sequence diagram of a sample analysis method in an embodiment;

14 is a flowchart of a sample analysis method in one embodiment;

Figure 15 is a comparison diagram of the test results of ultrasonic mixing and non-ultrasonic mixing in the sample analysis process in one embodiment;

16 is a flowchart of a sample analysis method in one embodiment;

Figure 17 is a flow diagram of a sample analysis method in one embodiment.

Detailed ways

The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments have been associated with similar element numbers. In the following embodiments, many details are described so that the present application can be better understood. However, those skilled in the art will readily recognize that some of the features may be omitted under different circumstances, or may be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application from being overwhelmed by excessive description, and for those skilled in the art, these are described in detail. Correlation operations are not necessary, and they can be completely de-correlated operations based on descriptions in the specification and general technical knowledge in the field.

Additionally, the features, acts, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a necessary order unless otherwise stated, a certain order must be followed.

The serial numbers themselves, such as "first", "second", etc., for the components herein are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "connection" mentioned in this application, unless otherwise specified, include both direct and indirect connections (connections).

For the immunoluminescence analyzer, the one-step test item in the present invention means that one test item only needs to be incubated in one step; correspondingly, the multi-step test item means that one test item needs to be incubated with multiple steps For example, a two-step test item means that the test item requires two-step incubation. First, add the reagents required for the first-step incubation to the sample, and then perform the first-step incubation. After the first-step incubation time is reached, then Add the reagents required for the second step of incubation, and then perform the second step of incubation. After the second step of incubation time, perform another magnetic separation, and then perform the measurement. Generally speaking, in a multi-step test project, magnetic separation needs to be performed after the last step of incubation before the measurement can be performed; while in a multi-step test project, in addition to the last step of incubation, whether magnetic separation is required after other steps of incubation The separation depends on factors such as the type of test items. For example, in a two-step test item, if magnetic separation is required after incubation in the first step of the test, the two-step test item can be called a two-step, two-separation test item. Magnetic separation is not required after incubation, so the two-step test item can be called a two-step-one-separation test item.

In a one-step test item or a multi-step test item, for each step of incubation or each incubation, the types of reagents that need to be added can be one or more, which are determined according to factors such as the type of test items When there are one-step or multi-step tests in a one-step test item or a multi-step test item, and there are multiple types of reagents to be added for incubation, this test item can be called a multi-component test project.

In one embodiment, a sample analyzer is provided. The sample analyzer is provided with an ultrasonic device, and the sample is ultrasonically mixed by the ultrasonic device to improve the detection accuracy. The sample analyzer may be a biochemical analyzer or an immunological analyzer, and this embodiment takes an immunoluminescence analyzer as an example for description.

1 and 2, the immunoluminescence analyzer mainly includes an ultrasonic device 10, a sample carrying mechanism 21, a sample dispensing mechanism 22, a reagent carrying mechanism 31, a reagent dispensing mechanism 32, a reaction mechanism 40, a magnetic separation mechanism 50 and a control device 60. The sample carrying mechanism 21 , the sample dispensing mechanism 22 , the reagent carrying mechanism 31 , the reagent dispensing mechanism 32 , the reaction mechanism 40 , the magnetic separation mechanism 50 and the ultrasonic device 10 are all installed on the base 100 , and the controller 60 is installed on the base 100 The controller 60 can also be installed on the base 100. The above-mentioned magnetic separation mechanism is not included in the biochemical analyzer.

The immunoluminescence analyzer also includes a cup-upper mechanism 71 , a cup-throwing position 72 , a first transfer mechanism 81 , a second transfer mechanism 82 and a measurement mechanism 90 mounted on the base 100 .

The reaction mechanism 40 is arranged in the middle, and the reagent carrying mechanism 31 , the magnetic separation mechanism 50 , the ultrasonic device 10 , the cup upper mechanism 71 , the cup throwing position 72 and the measuring mechanism 90 are respectively arranged around the reaction mechanism 40 .

The cup-upper mechanism 71 is used to store a new accommodating cup 103 that has not been used, and the accommodating cup 103 is also called a reaction cup. The cup-upper mechanism 71 itself also has a cup-shifting function, which can transfer the accommodating cup 103 from the storage position to the position to be grasped.

The first transfer mechanism 81 is arranged between the cup-upper mechanism 71 and the reaction mechanism 40 . The first transfer mechanism 81 is a cup grabbing mechanism, and the first transfer mechanism 81 is used to transfer the new accommodating cup 103 on the cup-upper mechanism 71 to a position close to the reaction mechanism. on the sample adding position 101 of the mechanism 40 , and transfer the accommodating cup 103 on the sample adding position 101 into the reaction mechanism 40 .

The cup throwing position 72 is located within the moving range of the first transfer mechanism 81 , the cup throwing position 72 is connected to the recovery box, and the cup throwing position 72 is used to recover the used accommodating cup 103 . The first transfer mechanism 81 is also used for transferring the accommodating cup 103 detected on the reaction mechanism 40 to the cup throwing position 72 .

The sample carrying mechanism 21 is used to carry the sample. In some examples, the sample carrying mechanism 21 may include a sample distribution module (SDM, Sample Delivery Module); in other examples, the sample carrying mechanism 21 may also be a sample tray, and the sample tray includes a plurality of sample positions such as sample tubes. By rotating the disk-like structure of the disk, the sample can be dispatched to a corresponding position, such as a position for the sample dispensing mechanism 22 to draw the sample.

The sample dispensing mechanism 22 includes a sampling needle, a moving mechanism and a driving pump. The moving mechanism is used to drive the sampling needle to move two-dimensionally or three-dimensionally between the sample carrying mechanism 21 and the sample adding position 101, and the driving pump is used to provide suction for the sampling needle. Kind and spit motivation. The sample dispensing mechanism 22 is used for sucking the sample in the sample tube on the sample carrying mechanism 21 , and for filling the sucked sample into the accommodating cup 103 on the sample filling position 101 .

The reagent carrying mechanism 31 is used for carrying reagents. In one embodiment, the reagent carrying mechanism 31 may be a reagent disk, the reagent disk is arranged in a disc-like structure and has multiple positions for carrying the reagent containers, and the reagent carrying mechanism 31 can rotate and drive the reagent containers it carries to rotate, It is used to rotate the reagent container to a specific position, for example, the position where the reagent is sucked by the reagent dispensing mechanism 32 . The number of reagent carrying mechanisms 31 may be one or more.

The reagent dispensing mechanism 32 includes a reagent needle, a moving mechanism and a driving pump. The moving mechanism is used to drive the reagent needle to move two-dimensionally or three-dimensionally between the reagent carrying mechanism 31 and the reaction mechanism 40, and the driving pump is used to provide the reagent needle with suction reagents And the power to spit reagents. The reagent dispensing mechanism 32 is used for absorbing the reagent in the reagent tube on the reagent carrying mechanism 31, and for filling the sucked reagent into the accommodating cup 103 containing the sample on the reaction mechanism 40, and the sample in the accommodating cup 103 and the reagent are mixed and reacted A reaction solution is formed.

The reaction mechanism 40 is used to provide an incubation place for the reaction liquid. The reaction mechanism 40 can be a reaction disk, which is arranged in a disc-shaped structure and has one or more placement positions for placing the reaction cup. The reaction disk can rotate and drive the reaction cup. The cuvette in the placement position is rotated for scheduling the cuvette in the reaction tray and incubating the reaction solution in the cuvette.

The magnetic separation mechanism 50 includes a cleaning liquid dispensing structure, a magnetic suction structure, a liquid suction structure and a substrate dispensing mechanism. The cleaning liquid dispensing structure is used to inject the cleaning liquid into the reaction solution after incubation, and the cleaning liquid is used to inject the cleaning liquid into the incubated reaction liquid. The free substances in the reaction solution after incubation are separated; the magnetic suction structure is used to perform the magnetic suction operation on the reaction solution filled with the cleaning solution, and the magnetic suction structure is used to adsorb the reaction complexes bound to the magnetic beads; the suction structure is used for The other components except the reaction complex bound to the magnetic beads are discharged out of the holding cup 103; the substrate dispensing mechanism is used to add the substrate into the reaction liquid in the holding cup 103, and the reaction between the substrate and the reaction liquid The complex reacts, and the substrate luminescently labels the reaction complex.

There are two magnetic separation mechanisms 50 , and the two magnetic separation mechanisms 50 can work independently of each other, so as to improve the efficiency of the test.

The second transfer mechanism 82 is installed between the reaction mechanism 40 and the magnetic separation mechanism 50, and a mixing position 102 is provided at a position close to the reaction mechanism 40 and the magnetic separation mechanism 50. Both the mixing position 102 and the sample adding position 101 are provided with For the cup holder on which the accommodating cup 103 is placed, the second transfer mechanism 82 is used to transfer the accommodating cup 103 among the reaction mechanism 40 , the magnetic separation mechanism 50 and the mixing station 102 .

The measurement mechanism 90 is used to perform optical measurement on the reaction solution after incubation to obtain reaction data of the sample. For example, the measuring mechanism 90 detects the luminescence intensity of the reaction solution to be measured, and calculates the concentration of the component to be measured in the sample based on the calibration curve.

The machine base 100 is further provided with a cleaning mechanism and a waste liquid suction mechanism, the cleaning mechanism is used for cleaning the sampling needle and the reagent needle, and the waste liquid suction mechanism is used for suctioning the detected reaction liquid.

The ultrasonic device 10 is set close to the sample adding position 101. The ultrasonic device 10 is used to perform ultrasonic mixing operation on the sample located in the holding cup 103 on the sample adding position 101. Ultrasonic mixing can make the components in the sample uniformly dispersed, so that the sample can be Fully react with the reagent, thereby improving the accuracy of the detection.

In one embodiment, the ultrasonic device 10 is disposed close to the reaction mechanism 40 , or the ultrasonic device 10 is installed in the reaction mechanism 40 .

Referring to FIG. 3, in an embodiment, a vortex mixing device 200 is also installed at the mixing position 102. The vortex mixing device 200 includes a driving motor 201, a transmission belt 202, an eccentric rotating shaft 203 and a mounting seat 204, etc. The driving motor 201 is fixedly installed on the mounting seat 204, the output shaft of the driving motor 201 is arranged downward, the eccentric rotating shaft 203 is rotatably installed on the mounting seat 204 through a bearing, the eccentric rotating shaft 203 is vertically arranged, and the eccentric rotating shaft 203 has a non-collinear first. One section and the second section, the first section is located at the lower position, the second section is located at the upper position, and the first and second sections of the eccentric shaft 203 are both parallel to the output shaft of the drive motor 201 . Pulleys are installed on the output shaft of the drive motor 201 and on the first section of the eccentric shaft 203, respectively. The drive belt 202 is connected to the pulley of the drive motor 201 and the eccentric shaft 203. The drive motor 201 drives the eccentric shaft 203 to rotate through the drive belt 202. The eccentric rotating shaft 203 is installed with a cup holder 205 for placing the holding cup 103 , and the eccentric rotating shaft 203 can drive the holding cup 103 located on the cup holder 205 to rotate eccentrically, and perform a vortex mixing operation on the reaction liquid in the holding cup 103 . The drive motor 201 is connected to the controller 60, and the controller 60 controls the output power and output duration of the drive motor 201 to realize various vortex mixing modes with different intensities and times. .

In this embodiment, the base 100 is provided with a diluent carrying mechanism 104, the diluent carrying mechanism 104 is used to carry the diluent, and the sample dispensing mechanism 22 is used to absorb the diluent on the diluent carrying mechanism 104, and draw the diluent The diluent is filled into the holding cup 103 with the sample on the sample filling position 101 . The diluent is used to dilute the sample with higher concentration, and reduce the concentration of the sample to an appropriate value, so that the sample can fully react with the reagent.

In one embodiment, a diluent dispensing mechanism is provided on the base 100 , the diluent dispensing mechanism replaces the sample dispensing mechanism 22 to add diluent, and the diluent dispensing mechanism is used to absorb the diluent on the diluent carrying mechanism 104 . diluent, and fill the sucked diluent into the holding cup 103 with the sample on the sample filling position 101 .

In this embodiment, the reagent carrying mechanism 31 is also used for carrying the pretreatment reagent, and the reagent dispensing mechanism 32 is also used for sucking the pretreatment reagent from the reagent carrying mechanism 31, and filling the pretreatment reagent into the accommodating cup 103 with the sample . Pretreatment reagents are different from other reagents. Pretreatment reagents do not participate in the reactions required by the test items. Pretreatment reagents are used to perform corresponding pretreatment on samples, so that the samples can fully react with reagents (such as magnetic bead reagents).

In one embodiment, the base 100 is provided with a pretreatment reagent carrying mechanism, and the pretreatment reagent carrying mechanism is specially used to carry the pretreatment reagent.

In one embodiment, the base 100 is provided with a pretreatment reagent dispensing mechanism, the pretreatment reagent dispensing mechanism replaces the reagent dispensing mechanism 32 to add pretreatment reagents, and the pretreatment reagent dispensing mechanism is used to absorb the reagent carrying mechanism. The pretreatment reagent on 31 is filled, and the sucked pretreatment reagent is filled into the holding cup 103 with the sample on the sample filling position 101 .

In this embodiment, the controller 60 is respectively connected with the ultrasonic device 10 , the sample dispensing mechanism 22 , the reagent dispensing mechanism 32 , the reaction mechanism 40 , the magnetic separation mechanism 50 , the first transfer mechanism 81 and the second transfer mechanism 82 . 60 is used to control the test sequence of the entire sample analyzer.

When the controller 60 controls the ultrasound device 10, the controller 60 obtains the test items input or selected by the doctor, obtains test item parameters corresponding to the test items, and matches an ultrasound mode from a plurality of ultrasound modes to the sample according to the test item parameters. Perform ultrasonic mixing.

Different ultrasonic modes have different ultrasonic mixing intensities or different ultrasonic mixing times. The mixing intensity is controlled by the input power. It can be set to include at least three ultrasonic mixing intensities: strong, medium and weak, and can be set to include at least 1s. and 2s two ultrasonic mixing times.

Ultrasonic mixing modes include at least the following:

In the first mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is medium, and the ultrasonic mixing time is 1 s;

In the second mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is weak, and the ultrasonic mixing time is 2s;

In the third mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 s.

The test item parameters include numbers, letters, or a combination of the two. For example, the test item parameter of the TNI (troponin) item is 2, and the test item parameter of the E2 (estradiol) item includes 0 and 1. The controller 60 pre-stores item test parameters corresponding to different test items, and each item test parameter corresponds to a mixing mode. If the test item parameter 0 corresponds to the first mixing mode, when the test item parameter obtained by the controller 60 is 0, the ultrasonic device 10 is driven to perform ultrasonic mixing with a medium intensity and a time of 1 s on the sample; the test item Parameter 1 corresponds to the second mixing mode. When the parameter of the test item obtained by the controller 60 is 1, the ultrasonic device 10 is driven to perform ultrasonic mixing with a weak intensity and a time of 2s on the sample; Corresponding to the third mixing mode, when the parameter of the test item acquired by the controller 60 is operation 2, the ultrasonic device 10 is driven to perform ultrasonic mixing with a strong intensity and a time of 1 s on the sample.

Different ultrasonic mixing modes can be set according to specific test items, so that the ultrasonic device 10 can effectively ultrasonically mix the samples in different test items.

In one embodiment, there are multiple ultrasonic devices 10 , and one ultrasonic device 10 is disposed near the mixing position 102 , so that the ultrasonic device 10 can ultrasonically mix the reaction solution in the holding cup 103 on the mixing position 102 .

Referring to FIG. 4 , in this embodiment, the ultrasonic device 10 is a device independent of other mechanisms, that is, the ultrasonic device 10 can operate independently. For example, the ultrasonic device 10 can operate independently of the sample dispensing mechanism 22 . Run synchronously or asynchronously to improve the efficiency of item detection.

The ultrasonic device 10 is a contact ultrasonic device. The ultrasonic device 10 includes an ultrasonic transducer 11, a transmission member 12 and a moving device 13. The ultrasonic transducer 11 includes a backing layer, a piezoelectric layer and a matching layer that are connected in sequence. The layer is a piezoelectric crystal, and the piezoelectric crystal produces compression and expansion in the thickness direction through the inverse piezoelectric effect under the action of the driving electrical signal. The frequency of this deformation reaches the ultrasonic frequency, forming ultrasonic vibration.

Please refer to FIG. 4 and FIG. 5 , the transmission member 12 is a solid rod-shaped structure, and the transmission member 12 has a first end and a second end, wherein the first end is the upper end, the second end is the lower end, and the first end of the transmission member 12 There is an external thread, the lower end of the ultrasonic transducer 11 is provided with an internal thread, and the transmission member 12 is installed on the lower end of the ultrasonic transducer 11 by means of screw connection. device 11 is connected. The transmission member 12 is a resonant rod, the transmission member 12 is connected with the matching layer of the ultrasonic transducer 11 , and the transmission member 12 is used for transmitting ultrasonic vibration. Compared with the transmission member 12 with a hollow structure, the solid transmission member 12 is conducive to the transmission of axial vibration, and when the outer diameter of the transmission member 12 decreases along the direction of ultrasonic vibration transmission, the solid transmission member 12 is conducive to the convergence of energy , in order to achieve better ultrasonic mixing effect.

The outer diameter of the transmission member 12 gradually decreases or decreases in steps from the first end to the second end, and the transmission member 12 has the function of concentrating energy. When the ultrasonic vibration is transmitted from the first end to the second end, the second end is opposite to the first end. The axial cross-sectional area of one end is reduced, and the ultrasonic vibration is more concentrated at the second end relative to the first end, so that the second end of the transmission member 12 amplifies the amplitude of the outgoing ultrasonic vibration relative to the first end, thereby increasing the outgoing ultrasonic energy. .

Specifically, the transmission member 12 includes a first end 121 , a middle section 122 and a second end 123 , wherein the first end 121 is a connection end with an external thread, the second end 123 is a needle bar structure, and the outer end of the second end 123 The diameter is smaller than the inner diameter of the receiving cup 103 so that the second end 123 of the transmission member 12 can be inserted into the receiving cup 103 . The middle section 122 is a horn-shaped structure, the end of the middle section 122 connected with the first end 121 is the big end of the horn, the end of the middle section 122 connected with the second end 123 is the small end of the horn, and the middle section 122 is from the big end of the horn to the small end of the horn. The shaft diameter of the end gradually decreases.

The middle section 122 can also be composed of one or any combination of cylindrical rods and conical rods. Please refer to FIG. 6 , the middle section 122 of structure a includes two sections of cylindrical rods with different diameters; the middle section 122 of structure b includes four sections of cylindrical rods with different diameters; the middle section 122 of structure c includes a section of conical rods; the middle section 122 of structure c includes a section of conical rods; The middle section 122 includes two sections of cylindrical rods of different diameters and a section of conical rods. The above five structures of the transmission member 12 are all structures that gradually decrease or stepwise decrease from the first end to the second end, and can play a role of amplifying the amplitude.

Referring to FIG. 7 , the mobile device 13 includes a mounting base 131 , a swing arm assembly 132 , a first moving assembly 133 and a second moving assembly 134 .

The swing arm assembly 132 includes a swing arm 1321 and a lift rod 1322, the lift rod 1322 is vertically movably liftable and rotatable mounted on the mounting seat 131, the swing arm 1321 is arranged horizontally, one end of the swing arm 1321 is connected to the lift rod 1322, and the ultrasonic The transducer 11 is mounted on one end of the swing arm 1321 away from the lift rod 1322 . The swing arm assembly 132 is used to drive the vertical lift and horizontal rotation of the ultrasonic transducer 11 and the transmission member 12 . In one embodiment, the swing arm 1321 and the lift rod 1322 can also be an integrated structure.

The first moving assembly 133 is a lifting assembly. The first moving assembly 133 includes a lifting motor 1331 and a lifting transmission assembly 1332. The lifting motor 1331 is installed on the mounting seat 131. The lifting transmission assembly 1332 includes a transmission wheel, a transmission belt, a gear and a rack, and the tooth The bar is vertically installed on the lifting rod 1322, the gear is rotatably installed on the mounting seat 131, the gear is meshed with the rack, the lifting motor 1331 is connected with the gear through a transmission wheel and a transmission belt, and the lifting motor 1331 drives the lifting rod through the rack and pinion. 1322 Lift and move. In one embodiment, the first moving component 133 is a linear motor, and the output shaft of the linear motor is directly connected with the lift rod 1322 , and can also drive the lift rod 1322 to move up and down.

The second moving assembly 134 is a rotating assembly. The second moving assembly 134 includes a rotating motor 1341 and a rotating transmission assembly 1342. The rotating motor 1341 is mounted on the mounting seat 131. The rotating transmission assembly 1342 includes a transmission belt and a rotating gear. The gear is sleeved on the lifting rod 1322, the rotating gear is connected with the lifting rod 1322 by a key, the lifting rod 1322 can move up and down relative to the rotating gear, the rotating gear is used to drive the lifting rod 1322 to rotate, the rotating motor 1341 is connected with the rotating gear through a transmission belt, and the rotating motor 1341 is used to drive the lift rod 1322 to rotate. In one embodiment, the rotating motor 1341 is connected to the lifting rod 1322 through a gear set, and can also drive the lifting rod 1322 to rotate.

In one embodiment, the moving device 13 only includes the mounting base 131 , the swing arm assembly 132 and the first moving assembly 133 , the ultrasonic device 10 has a lifting function, and the ultrasonic device 10 is used to perform an operation on the sample 104 in the accommodating cup 103 at a specific position. Mixing operation.

In one embodiment, the second moving component 134 can also be a plane moving component composed of X-axis movement and Y-axis movement. The member 12 moves alternately among the plurality of mixing positions 102 .

Referring to FIG. 8 , in this embodiment, the transmitting member 12 of the ultrasonic device 10 is directly inserted into the sample 104 of the accommodating cup 103 . The ultrasonic device 10 has a preset frequency and voltage, so that the ultrasonic vibration mainly propagates along the axial direction, and the second end face of the transmitting member 12 is the emitting surface of the ultrasonic wave. During ultrasonic mixing, the second end face of the transmission member 12 emits ultrasonic waves into the sample 104, an ultrasonic sound field is formed in the sample 104, and the sample 104 will form a violent liquid flow under the action of the ultrasonic sound field to realize the various components in the sample 104. of mixing.

In addition to the vibration effect of the ultrasonic waves, which can realize the mixing of the sample 104 , the cavitation effect generated by the ultrasonic waves in the liquid can also uniformly disperse some agglomerated and adhered substances in the sample 104 . When the frequency and sound pressure of the ultrasonic waves are controlled, combined with the amplification effect of the transmission member 12, the ultrasonic energy entering the sample 104 of the holding cup 103 is greater than the threshold of ultrasonic cavitation, then during the ultrasonic mixing process, the energy in the sample 104 can be Ultrasonic cavitation occurs. When ultrasonic cavitation occurs, a large amount of energy will be released to generate a certain force on some agglomerated and adhered substances in the sample 104 to disperse them. At the same time, under the action of ultrasonic vibration and mixing, these substances can be uniformly dispersed in the reaction. in the cup.

In one embodiment, the ultrasonic device 10 is a non-contact ultrasonic mixing, the ultrasonic device 10 is in contact with the holding cup 103 , and the ultrasonic waves emitted by the ultrasonic device 10 are transmitted to the sample in the holding cup 103 through the holding cup 103 .

Please refer to FIG. 9 and FIG. 10 , the ultrasonic device 10 includes an ultrasonic transducer 11 and a transmission member 12 . During ultrasonic mixing, the transmission member 12 abuts the second end against the outer wall of the accommodating cup 103 , and transmits the ultrasonic vibration into the sample 104 through the accommodating cup 103 . Since the transmission member 12 does not need to be inserted into the receiving cup 103, the axial length of the transmission member 12 is shorter than that of the contact type transmission member, but it also has a gradual or stepped reduction from the first end to the second end. features to achieve amplified amplitude.

During ultrasonic mixing, the second end face of the transmission member 12 in this embodiment abuts against the outer wall of the accommodating cup 103, and the part of the outer wall of the accommodating cup 103 in contact with the transmission member 12 is the part surrounding the sample 104, so as to convert the ultrasonic The ultrasonic vibration generated by the energy generator 11 is transmitted to the liquid in the holding cup 103 . The part of the accommodating cup 103 surrounding the sample 104 is the bottom of the accommodating cup 103 and the lower end sidewall connected to the bottom, so the second end of the transmission member 12 abuts against the bottom of the accommodating cup 103 and any position of the lower end sidewall connected to the bottom Both can transmit ultrasonic vibration to the liquid in the holding cup 103 .

In this embodiment, the ultrasonic device 10 is a movable structure. The ultrasonic device 10 further includes a moving device. The moving device includes a mounting seat and a horizontal moving component. The horizontal moving component is installed on the mounting seat, and the ultrasonic transducer is installed on the horizontal moving component. , the horizontal moving component is an air cylinder or a linear motor, and the horizontal moving component is used to drive the second end of the transmission member 12 to abut or leave the outer wall of the accommodating cup 103 on the mixing position 102 .

In one embodiment, the ultrasonic device 10 is set as a fixed structure, and the transmission member 12 is located at a preset position, so that after the accommodating cup 103 is placed on the mixing station 102 , the accommodating cup 103 will directly contact the second end of the transmission member 12 .

In this embodiment, the sample analyzer further includes a holding device 110 , and the holding device 110 is used to limit the radial degree of freedom of the accommodating cup 103 on the mixing position 102 .

Please refer to FIG. 11 and FIG. 12 , the gripping device 110 includes two oppositely arranged gripping assemblies, each gripping assembly includes a gripping motor 111 , a gripping cam 112 and a gripping clamping block 113 , and the gripping motor 111 is installed on the On the machine base 100, the output shaft of the gripping motor 111 is arranged vertically upward, the output shaft of the gripping motor 111 is connected to the gripping cam 112 in a transmission connection, the gripping cam 112 is arranged horizontally, and the gripping cam 112 is connected with the gripping clamping block 113. In contact connection, the holding and clamping block 113 is installed on the machine base 100 movably horizontally. If the accommodating cup 103 is a circular tube, the surface of the clamping block 113 facing the accommodating cup 103 is a concave arc surface; if the accommodating cup 103 is a square tube, the surface of the clamping block 113 facing the accommodating cup 103 is a flat surface. The convex portion of the gripping cam 112 is a convex arc surface, so the surface of the gripping block 113 facing the gripping cam 112 is a concave arc surface with a larger curvature, so that the concave arc surface of the gripping block 113 can guide the gripping cam 112. The raised portion of the tightening cam 112 slides in and out. The gripping motor 111 is used to drive the gripping cam 112 to rotate, so that the gripping cam 112 drives the gripping clamping block 113 to approach or move away from the accommodating cup 103 . When they are on a line, the two holding clamp blocks 113 hold the holding cup 103 tightly, and the radial freedom of the holding cup 103 is limited, thereby avoiding the shaking of the holding cup 103 during the ultrasonic mixing process and ensuring the holding cup 103 Good contact with the transfer element 12 .

In this embodiment, since the ultrasonic device 10 is in contact with the lower end of the holding cup 103 to realize ultrasonic mixing, the holding clamping block 113 of the holding device 110 holds the side wall of the lower end of the holding cup 103 tightly to improve the holding stability . When the transmission member 12 of the ultrasonic device 10 abuts on the lower end sidewall of the accommodating cup 103 , the transmission member 12 and the clamping block 113 are arranged staggered from each other on the lower end sidewall of the accommodating cup 103 .

In one embodiment, the gripping device 110 may also include a linear driving member and a gripping clamping block. The linear driving member is an air cylinder or a linear motor. The limit of the accommodating cup 103 .

This embodiment adopts the non-contact ultrasonic device 10 , which can also transmit ultrasonic vibration to the sample 104 in the holding cup 103 to form an ultrasonic sound field and ultrasonic cavitation, so as to ultrasonically mix the sample 104 in the holding cup 103 .

In this embodiment, both the contact and non-contact ultrasonic devices 10 are independent of the sample dispensing mechanism 22 . The ultrasound device 10 and the sample dispensing mechanism 22 are two spaced apart components.

In one embodiment, the ultrasonic device 10 and the sample dispensing mechanism 22 are connected in an integrated structure. The ultrasonic device 10 includes an ultrasonic transducer, the ultrasonic transducer is arranged on the moving mechanism of the sample dispensing mechanism 22, the ultrasonic transducer is connected with the sampling needle, and the ultrasonic vibration generated by the ultrasonic transducer is transmitted to the sampling needle, and the sampling needle is regenerated. Send ultrasound into the sample. After the ultrasonic device 10 and the sample dispensing mechanism 22 are combined, the sampling needle can not only absorb the sample and the diluent, but also can be used to perform an ultrasonic mixing operation on the sample. Specifically, the sampling needle can perform an ultrasonic mixing operation on the sample in the sample container on the sample carrying mechanism 21 , and can perform an ultrasonic mixing operation on the sample in the accommodating cup 103 on the sample adding position 101 .

In one embodiment, the ultrasonic device 10 is disposed close to the sample carrying mechanism 21 , or the ultrasonic device 10 is disposed on the sample carrying mechanism 21 .

In one embodiment, a sample analysis method is provided, and the sample analysis method is performed by the sample analyzer in the above embodiment.

Please refer to Figure 13. In the whole machine test, according to different reagent items, the sample analyzer mainly includes the following five different test procedures:

Test process 1. One-step separation: After adding sample S and reagent R respectively, carry out one incubation and one magnetic separation operation, and then carry out adding substrate A, incubation and photometry.

Test process 2. Two-step method for one-time separation: after adding sample S, add reagent R1 in the first step. Reagent R1 is one or more reagents, and the sample and reagent R1 are mixed to form a reaction solution for the first incubation; After the first incubation, the second step is to add reagent R2, which is one or more reagents, and the reagent R2 and the reaction solution after the first incubation form a new reaction solution for the second incubation; the second incubation The resulting reaction solution was subjected to magnetic separation, substrate A addition, incubation and photometry in sequence.

Test process 3. Two-step separation: after adding the sample, add reagent R1 in the first step, mix the sample and reagent R1 to form a reaction solution for the first incubation, and perform the first magnetic separation after the first incubation Operation; after the first magnetic separation operation, the second step is to add reagent R2, and the reagent R2 and the reaction solution after the first magnetic separation form a new reaction solution for the second incubation; the second incubation reaction solution is subjected to the first step. The second magnetic separation operation; the reaction solution after the second magnetic separation operation is sequentially added with substrate A, incubated and photometric.

Test process 4. Sample pretreatment: add sample S, then add pretreatment reagent, pretreatment reagent preprocesses the sample to form sample S'; add reagent R to the pretreated sample S', and then proceed in sequence Incubation, magnetic separation, addition of substrate A, incubation and photometry.

Test process 5. Sample pretreatment: add sample S, then add diluent, and the diluent will dilute the sample to obtain sample S' with lower concentration; add reagents to the diluted sample S', and then proceed in sequence Incubation, magnetic separation, addition of substrate A, incubation and photometry.

In this embodiment, the sample analysis method is controlled and executed by the controller 60, and the one-step magnetic separation is taken as an example for description. In this sample analysis method, ultrasonic mixing is performed on the sample after adding the sample.

Please refer to FIG. 14 , the sample analysis method of this embodiment includes the following steps:

S101: add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S102: ultrasonic mixing;

The ultrasonic device 10 transmits ultrasonic waves into the sample on the sample adding position 101, and performs an ultrasonic mixing operation on the sample. The ultrasonic device 10 uniformly disperses each component in the sample, so that the sample can fully react with the reagent.

The principle that the controller 60 controls the ultrasonic device 10 to perform ultrasonic mixing is as follows:

The controller 60 acquires the test items input or selected by the doctor, and acquires the test item parameters corresponding to the test items, and matches one ultrasonic mode from a plurality of ultrasonic modes according to the test item parameters to perform an ultrasonic mixing operation on the sample. Performed by the ultrasound device 10 .

Different ultrasonic modes have different ultrasonic mixing intensities or different ultrasonic mixing times. The mixing intensity is controlled by the input power. Three ultrasonic mixing intensities can be set: strong, medium and weak, or more or less. Ultrasonic mixing intensity gradient selection, you can set two ultrasonic mixing times of 1s and 2s.

Ultrasonic mixing modes include at least the following:

In the first mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is medium, and the ultrasonic mixing time is 1 s;

In the second mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is weak, and the ultrasonic mixing time is 2s;

In the third mixing mode, the ultrasonic device 10 is used to perform the ultrasonic mixing operation, the ultrasonic mixing intensity is strong, and the ultrasonic mixing time is 1 s.

The test item parameters include numbers, letters, or a combination of the two. For example, the test item parameter of the TNI (troponin) item is 2, and the test item parameter of the E2 (estradiol) item includes 0 and 1. The controller 60 pre-stores project test parameters corresponding to different test items, and each project test parameter corresponds to a mixing mode. For example, the test item parameter 0 corresponds to the first mixing mode. When the test item obtained by the controller 60 When the parameter is 0, the ultrasonic device 10 is driven to perform ultrasonic mixing with a medium intensity and a time of 1 s; the test item parameter 1 corresponds to the second mixing mode, when the test item parameter obtained by the controller 60 is 1 , then drive the ultrasonic device 10 to perform ultrasonic mixing with a weak intensity and a time of 2 s on the sample; the test item parameter 2 corresponds to the third mixing mode, and when the test item parameter obtained by the controller 60 is operation 2, Then, the ultrasonic device 10 is driven to perform ultrasonic mixing with a strong intensity and a time of 1 s on the sample.

Different ultrasonic mixing modes can be set according to specific test items, so that the ultrasonic device 10 can effectively ultrasonically mix the samples in different test items.

S103: add reagent;

The first transfer mechanism 81 transfers the accommodating cup 103 containing the sonicated sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the accommodating cup 103 to which the reagent R needs to be added to the reagent adding position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sonicated sample S and the reagent R in the accommodating cup 103 mixed to form a reaction solution.

S104: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S105: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S106: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the ultrasonically mixed reaction solution from the mixing position 102 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S107: Add substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 dispenses the substrate A into the holding cup 103 after liquid aspiration, and the substrate A carries out luminescence labeling on the reaction complex in the reaction solution.

S108: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

S109: light measurement;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In order to verify the effectiveness of this sample analysis method in improving the accuracy of detection, the following verification work is carried out:

Referring to Figure 15, there are different substances in the sample, including interfering substances, this validation was used to study the effect of interfering substances on the final measured value. For the same batch of model samples, use the methods with and without ultrasonic mixing for clinical testing, ultrasonically process the samples after adding the samples and before adding the reagents, compare the final test results, and compare with the sample's Comparison of standard test results for centrifugation supernatants. It can be seen from the comparison of test results that after ultrasonic mixing, the test of the model sample is no longer closer to the real value of the sample. That is, ultrasonically mixing the reaction solution can improve the mixing effect and make the measured value more accurate.

In an embodiment, in step 102, an ultrasonic mixing operation is performed on the sample on the reaction mechanism 40, and the specific steps are as follows:

The first transfer mechanism 81 transfers the accommodating cup 103 containing the sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40 , and the ultrasonic device 10 performs an ultrasonic mixing operation on the sample in the reaction mechanism 40 .

The method can also perform ultrasonic mixing operation on the sample before adding the reagent, so that the sample and the reagent can fully react.

In an embodiment, ultrasonic mixing is performed in step 104, and the ultrasonic device 10 performs ultrasonic mixing on the reaction solution in the holding cup 103 on the mixing position 102, which can achieve a better mixing effect, so that the sample S can be mixed. Fully react with reagent R.

In one embodiment, a sample analysis method is provided. The difference between this sample analysis method and the sample analysis method in the above-mentioned embodiment is that the sample is first subjected to ultrasonic mixing operation, and then the sample is added.

During the ultrasonic operation of the sample: the ultrasonic device 10 transmits ultrasonic waves into the sample in the sample container on the sample carrying mechanism 21, and performs an ultrasonic mixing operation on the sample.

The operation of ultrasonic mixing is the same as the above-mentioned embodiment, and different ultrasonic modes are matched for different test items to effectively mix the samples.

After the ultrasonication is completed, the sample dispensing mechanism 22 sucks the ultrasonicated sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

In this embodiment, the ultrasonic mixing operation is performed on the sample in the sample container on the sample carrying mechanism 21, which can also uniformly disperse the components of the sample, which is conducive to the sufficient reaction between the sample and the reagent.

In one embodiment, a sample analysis method is provided. The difference between the sample analysis method and the sample analysis method in the above-mentioned embodiment is that ultrasonic mixing is performed on the sample filled with the diluent.

Please refer to FIG. 16 , the sample analysis method of this embodiment includes the following steps:

S201: Add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S202: add diluent;

The sample dispensing mechanism 22 sucks the diluent from the diluent carrying mechanism 104, and fills the sucked diluent into the holding cup 103 with the sample on the sample filling position 101. The concentration is reduced to an appropriate value to allow the sample to react adequately with the reagents.

S203: ultrasonic mixing;

The ultrasonic device 10 transmits ultrasonic waves into the sample filled with diluent on the sample adding position 101, and performs an ultrasonic mixing operation on the sample filled with diluent. The ultrasonic device 10 uniformly disperses the components in the sample, so that the sample filled with the diluent can fully react with the reagent.

The operation of ultrasonic mixing is the same as that of the above-mentioned embodiment, and different ultrasonic modes are matched for different test items, so as to effectively mix the samples filled with diluent.

S204: add reagent;

The first transfer mechanism 81 transfers the accommodating cup 103 containing the ultrasonically filled sample S with the diluent from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the accommodating cup 103 to which the reagent R needs to be added to the reagent addition position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sonicated sample S and the reagent R in the accommodating cup 103 mixed to form a reaction solution.

S205: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S206: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S207: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the ultrasonically mixed reaction solution from the mixing position 102 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S208: Add substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 dispenses the substrate A into the holding cup 103 after liquid aspiration, and the substrate A carries out luminescence labeling on the reaction complex in the reaction solution.

S209: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

S210: light measurement;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In this embodiment, for a sample with an excessively high concentration, dilution is performed first, and then ultrasonic mixing is performed, which can not only improve the dilution effect, but also uniformly disperse various substances in the sample.

In one embodiment, a sample analysis method is provided. The difference between the sample analysis method and the sample analysis method in the above-mentioned embodiment is that ultrasonic mixing is performed on the sample filled with the pretreatment reagent.

Please refer to FIG. 17 , the sample analysis method of this embodiment includes the following steps:

S301: Add sample;

The first transfer mechanism 81 transfers the new accommodating cup 103 on the cup-upper mechanism 71 to the sample adding position 101;

The sample dispensing mechanism 22 sucks the sample S from the sample carrying mechanism 21 , and fills the sucked sample S into the accommodating cup 103 on the sample adding position 101 .

S302: add pretreatment reagent;

The first transfer mechanism 81 transfers the accommodating cup 103 containing the sonicated sample S from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the accommodating cup 103 to which the reagent R needs to be added to the reagent adding position;

The reagent dispensing mechanism 32 sucks the pretreatment reagent from the reagent carrying mechanism 31, and fills the sucked pretreatment reagent into the accommodating cup 103 with the sample on the sample filling position 101, and the pretreatment reagent is used for the corresponding pretreatment of the sample. Treated so that the sample can react sufficiently with reagents (eg, magnetic bead reagents).

S303: ultrasonic mixing;

The first transfer mechanism 81 transfers the accommodating cup 103 containing the sonicated sample S from the reaction mechanism 40 to the sample adding position 101;

The ultrasonic device 10 transmits ultrasonic waves into the sample filled with the pretreatment reagent on the sample adding position 101, and performs an ultrasonic mixing operation on the sample filled with the pretreatment reagent. The ultrasonic device 10 uniformly disperses the components in the sample, so that the sample filled with the pretreatment reagent can fully react with the reagent.

The operation of ultrasonic mixing is the same as that of the above-mentioned embodiment, and different ultrasonic modes are matched for different test items, so as to effectively mix the samples added with the pretreatment reagent.

S304: add reagent;

The first transfer mechanism 81 transfers the accommodating cup 103 containing the sample S filled with the ultrasonic pretreatment reagent from the sample adding position 101 to the outer ring in the reaction mechanism 40; the reaction mechanism 40 transfers the accommodating cup 103 to which the reagent R needs to be added Transfer to the reagent addition position;

The reagent dispensing mechanism 32 sucks the reagent R from the reagent carrying mechanism 31 , and fills the sucked reagent into the accommodating cup 103 at the reagent loading position in the reaction mechanism 40 , and the sonicated sample S and the reagent R in the accommodating cup 103 mixed to form a reaction solution.

S305: vortex mixing;

The second transfer mechanism 82 transfers the holding cup 103 containing the reaction solution to the mixing position 102;

A vortex mixing device is used to perform a vortex mixing operation on the reaction solution in the holding cup 103, so that the sample S and the reagent R can fully react.

S306: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the vortex-mixed reaction solution from the mixing position 102 back to the inner circle of the reaction mechanism 40 for incubation for a preset time.

S307: Magnetic separation;

The second transfer mechanism 82 transfers the holding cup 103 containing the ultrasonically mixed reaction solution from the mixing position 102 to the magnetic separation mechanism 50;

The cleaning liquid dispensing structure of the magnetic separation mechanism 50 fills the cleaning liquid into the holding cup 103 with the reaction liquid;

The magnetic attraction structure adsorbs the reaction complex with the magnetic beads in the holding cup 103 through the magnetic field;

The liquid suction structure discharges other substances and liquids except the adsorbed reaction complexes out of the holding cup 103 .

S308: Add substrate;

The substrate dispensing mechanism of the magnetic separation mechanism 50 dispenses the substrate A into the holding cup 103 after liquid aspiration, and the substrate A carries out luminescence labeling on the reaction complex in the reaction solution.

S309: incubation;

The second transfer mechanism 82 transfers the accommodating cup 103 containing the reaction solution injected with the substrate A from the magnetic separation mechanism 50 back to the outer ring of the reaction mechanism 40 for incubation.

S310: photometric;

The reaction mechanism 40 transfers the accommodating cup 103 of the incubated reaction solution to the detection position;

The measuring mechanism 90 detects the light-emitting reaction complex in the holding cup 103;

After completing the optical measurement, the waste liquid suction mechanism sucks the reaction liquid from the holding cup 103;

The first transfer mechanism 81 transfers the accommodating cup 103 from which the reaction liquid is discharged from the reaction mechanism 40 to the cup throwing position 72 .

In this embodiment, for a sample that needs to be pretreated, preprocessing is performed first, and then ultrasonic mixing is performed, which can not only improve the preprocessing effect, but also uniformly disperse various substances in the sample.

In an embodiment, the ultrasonic mixing in step 403 is performed on the reaction mechanism 40, and the specific steps are as follows:

The ultrasonic device 10 transmits ultrasonic waves into the sample filled with the pretreatment reagent in the reaction mechanism 40, and performs an ultrasonic mixing operation on the sample filled with the pretreatment reagent.

Ultrasonic mixing on the reaction mechanism 40 can also uniformly disperse the components of the sample filled with the pretreatment reagent, and the operation step of transferring the holding cup 103 to the sample adding position 101 can also be omitted, which saves the transfer time, thereby improving the detection efficiency.

In an embodiment, in the sample analysis method, diluent and pretreatment reagent are added to the sample successively, and the sample added with diluent is ultrasonically mixed, and the sample added with diluent and pretreatment reagent is ultrasonically mixed. uniform.

The method steps of the sample analysis method are: on the basis of steps 301 to 310 , the above-mentioned steps 402 and 403 are inserted into the method steps formed between steps 303 and 304 .

In the sample analysis method, the ultrasonic device successively performs ultrasonic mixing on the sample filled with diluent, the sample filled with diluent and pretreatment reagent, which can improve the dilution effect and pretreatment effect respectively, and uniformly disperse the components of the sample. Make the sample and reagent fully react, thereby improving the accuracy of detection.

The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention, and are not intended to limit the present invention. For those skilled in the art to which the present invention pertains, according to the idea of the present invention, several simple deductions, modifications or substitutions can also be made.

Claims (30)

1. A sample analyzer, characterized in that, comprising: A sample dispensing mechanism for drawing the sample and discharging the sample into the holding cup; The reagent dispensing mechanism is used to draw the reagent and discharge the reagent into the holding cup; The reaction mechanism is used to provide an incubation place for the reaction solution in the holding cup, and the reaction solution is formed by mixing the sample and the reagent; The measuring mechanism is used to measure the reaction solution; an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample in the holding cup. 2. The sample analyzer according to claim 1, wherein the controller is used to obtain test item parameters, and according to the test item parameters, match a kind of ultrasonic pattern pair from preset multiple ultrasonic patterns. The sample is subjected to ultrasonic mixing. 3. The sample analyzer according to claim 2, wherein the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times respectively. 4. The sample analyzer of claim 1, wherein the sample dispensing mechanism comprises a sampling needle, the ultrasonic device comprises an ultrasonic transducer, and the ultrasonic transducer is connected to the sampling needle, The sampling needle is used to transmit the ultrasonic vibration generated by the ultrasonic transducer into the sample. 5. The sample analyzer according to claim 1, wherein the ultrasonic device comprises an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to form ultrasonic vibration, and the transmission member has a first one end and a second end, the first end of the transmission member is connected to the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the accommodating cup; the moving device is connected to the ultrasonic transducer The moving device is used to drive the ultrasonic transducer and the transmitting member to move relative to the accommodating cup, and the second end of the transmitting member can be inserted into the sample in the accommodating cup, so as to transmit the ultrasonic Ultrasonic vibrations generated by the transducer are transmitted to the sample within the holding cup. 6. The sample analyzer of claim 1, wherein the ultrasonic device comprises an ultrasonic transducer and a transmission member, the ultrasonic transducer is used to generate ultrasonic vibration, the transmission member has a first end and The second end, the first end of the transmission member is connected with the ultrasonic transducer; the second end of the transmission member is used to abut against the outer wall of the accommodating cup, and the outer wall of the accommodating cup is in contact with the transmission member The part is the part surrounding the sample, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the sample in the accommodating cup. 7 . The sample analyzer according to claim 5 or 6 , wherein the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases in steps from the first end to the second end. 8 . . 8. A sample analyzer, comprising: a sample carrying mechanism for carrying a sample container containing a sample; a sample dispensing mechanism for sucking the sample in the sample container on the sample carrying mechanism and discharging the sample into the holding cup; The reagent dispensing mechanism is used to draw the reagent and discharge the reagent into the holding cup; The reaction mechanism is used to provide an incubation place for the reaction solution in the holding cup, and the reaction solution is formed by mixing the sample and the reagent; The measuring mechanism is used to measure the reaction solution; an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample in the sample container. 9. The sample analyzer according to claim 8, wherein the controller is used to obtain test item parameters, and according to the test item parameters, match an ultrasonic mode pair from preset multiple ultrasonic modes The sample is subjected to ultrasonic mixing. 10 . The sample analyzer of claim 9 , wherein the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times, respectively. 11 . 11. The sample analyzer of claim 8, wherein the sample dispensing mechanism comprises a sampling needle, the ultrasonic device comprises an ultrasonic transducer, and the ultrasonic transducer is connected to the sampling needle, The sampling needle is used to transmit the ultrasonic vibration generated by the ultrasonic transducer into the sample. 12. The sample analyzer according to claim 8, wherein the ultrasonic device comprises an ultrasonic transducer, a transmission member and a moving device, the ultrasonic transducer is used to generate ultrasonic vibration, and the transmission member has a first one end and a second end, the first end of the transmission member is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the sample container; the moving device is connected to the ultrasonic transducer The moving device is used to drive the ultrasonic transducer and the transfer member to move relative to the sample container, and the second end of the transfer member can be inserted into the sample in the sample container, so as to transmit the ultrasonic wave to the sample container. Ultrasonic vibrations generated by the transducer are transmitted to the sample within the sample container. 13. The sample analyzer of claim 8, wherein the ultrasonic device comprises an ultrasonic transducer and a transmission member for generating ultrasonic vibrations, the transmission member having a first end and a transmission member. The second end, the first end of the transmission member is connected with the ultrasonic transducer; the second end of the transmission member is used to abut against the outer wall of the sample container, and the outer wall of the sample container is in contact with the transmission member The part is the part surrounding the sample, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the sample in the sample container. 14. The sample analyzer according to claim 12 or 13, wherein the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases stepwise from the first end to the second end . 15. A sample analyzer, comprising: a dispensing mechanism for drawing the sample and discharging the sample into the holding cup, the dispensing mechanism is also used for drawing the pretreatment liquid and discharging the pretreatment liquid into the holding cup containing the sample; an ultrasonic device for generating ultrasonic vibrations to form ultrasonic waves; and A controller is connected to the ultrasonic device, and the controller is used for controlling the ultrasonic device to emit ultrasonic waves into the sample injected with the pretreatment liquid. 16. The sample analyzer of claim 15, wherein the pretreatment solution comprises at least one of a diluent and a pretreatment reagent. 17. The sample analyzer according to claim 16, wherein the dispensing mechanism comprises a sample dispensing mechanism and a reagent dispensing mechanism, The sample dispensing mechanism is used for sucking the sample and discharging the sample into the accommodating cup, and the sample dispensing mechanism is also used for sucking the diluent and discharging the diluent into the accommodating cup with the sample; The reagent dispensing mechanism is used to draw the pretreatment reagent and discharge the pretreatment reagent into the holding cup with the sample. 18. The sample analyzer according to claim 15, wherein the controller is used to obtain test item parameters, and according to the test item parameters, match an ultrasonic mode pair from preset multiple ultrasonic modes. The sample injected with the pretreatment reagent is subjected to ultrasonic mixing. 19. The sample analyzer of claim 18, wherein the plurality of ultrasonic modes have different ultrasonic intensities and/or ultrasonic action times, respectively. 20. The sample analyzer of claim 17, wherein the sample dispensing mechanism comprises a sampling needle, the ultrasonic device comprises an ultrasonic transducer, and the ultrasonic transducer is connected to the sampling needle, The sampling needle is used for transmitting the ultrasonic vibration generated by the ultrasonic transducer into the sample injected with the pretreatment liquid. 21. The sample analyzer of claim 15, wherein the ultrasonic device comprises an ultrasonic transducer, a transmission member, and a moving device, the ultrasonic transducer is used to generate ultrasonic vibration, and the transmission member has a first one end and a second end, the first end of the transmission member is connected with the ultrasonic transducer, the outer diameter of the second end of the transmission member is smaller than the inner diameter of the sample container; the moving device is connected to the ultrasonic transducer The moving device is used for driving the ultrasonic transducer and the transmission member to move relative to the sample container, and the second end of the transmission member can be inserted into the sample injected with the pretreatment liquid in the sample container, The ultrasonic vibration generated by the ultrasonic transducer is transmitted to the sample injected with the pretreatment liquid in the sample container. 22. The sample analyzer of claim 15, wherein the ultrasonic device comprises an ultrasonic transducer and a transmission member for generating ultrasonic vibrations, the transmission member having a first end and The second end, the first end of the transmission member is connected with the ultrasonic transducer; the second end of the transmission member is used to abut against the outer wall of the sample container, and the outer wall of the sample container is in contact with the transmission member The part of the sample is the part surrounding the sample injected with the pretreatment liquid, so as to transmit the ultrasonic vibration generated by the ultrasonic transducer to the sample injected with the pretreatment liquid in the sample container. 23. The sample analyzer according to claim 21 or 22, wherein the transmission member is a solid structure, and the outer diameter of the transmission member gradually decreases or decreases stepwise from the first end to the second end . 24. A sample analysis method, comprising the steps of: The sample dispensing mechanism injects the sample into the holding cup; The ultrasonic device emits ultrasonic waves into the sample of the holding cup; The reagent dispensing mechanism injects the reagent into the holding cup, and the reagent and the ultrasonicated sample form a reaction solution; The reaction solution is incubated in the reaction mechanism; The measuring mechanism performs optical measurement on the reaction solution. 25. The sample analysis method according to claim 24, wherein the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps: Get test project parameters; According to the test item parameters, an ultrasonic mode is matched from a plurality of preset ultrasonic modes to perform an ultrasonic mixing operation on the sample. 26. The sample analysis method according to claim 25, wherein the plurality of ultrasonic modes respectively have different ultrasonic intensities and/or ultrasonic action times. 27. The sample analysis method according to any one of claims 24 to 26, wherein the step of injecting the sample into the holding cup by the sample dispensing mechanism and the step of transmitting ultrasonic waves into the sample in the holding cup In between, the following steps are also included: At least one of a diluent and a pretreatment reagent is injected into the holding cup with the sample. 28. A sample analysis method, comprising the steps of: The ultrasonic device emits ultrasonic waves into the sample in the sample container on the sample carrying mechanism; The sample dispensing mechanism sucks the sonicated sample in the sample container on the sample carrying mechanism, and injects the sonicated sample into the holding cup; The reagent dispensing mechanism injects the reagent into the holding cup; The reaction solution is incubated in the reaction mechanism, and the reaction solution is formed by mixing the reagent and the sample after ultrasonication; The measuring mechanism performs optical measurement on the reaction solution. 29. The sample analysis method according to claim 28, wherein the ultrasonic mixing operation of the ultrasonic device is controlled by the following steps: Get test project parameters; According to the test item parameters, an ultrasonic mode is matched from a plurality of preset ultrasonic modes to perform an ultrasonic mixing operation on the sample. 30. The sample analysis method according to claim 29, wherein the plurality of ultrasonic modes respectively have different ultrasonic intensities and/or ultrasonic action times.

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