CN114174826A - Animal blood cell analysis method, analyzer and storage medium - Google Patents
Animal blood cell analysis method, analyzer and storage medium Download PDFInfo
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- CN114174826A CN114174826A CN201980098599.8A CN201980098599A CN114174826A CN 114174826 A CN114174826 A CN 114174826A CN 201980098599 A CN201980098599 A CN 201980098599A CN 114174826 A CN114174826 A CN 114174826A
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Abstract
An animal blood cell analysis method applied to an animal blood cell analyzer (100), the method comprising: selecting a target measurement mode from a plurality of animal measurement modes, and determining a target dosage of the diluent according to the target measurement mode (401); mixing the target dose of diluent with the blood of the animal to be detected and performing hemolysis treatment to obtain a detection sample (402); the test sample is tested for white blood cell count and classification (403). An animal blood cell analyzer (100) and a storage medium are also disclosed.
Description
The embodiment of the invention relates to an animal blood cell analysis technology, and relates to but is not limited to an animal blood cell analysis method, an animal blood cell analyzer and a storage medium.
The main principle of animal blood cell analysis is to test the parameters of white blood cells, red blood cells, platelets and the like of a diluted animal blood sample through micropores.
On an animal blood cell analyzer, blood samples of various animals such as dogs, cats, rats, mice, rabbits, etc. are required to be tested, but the hemolysis process of the leukocyte channel of each animal is different. Therefore, in the related art, the accuracy of the white blood cell count and classification result cannot be guaranteed in one animal blood cell analyzer.
Disclosure of Invention
The embodiment of the invention provides an animal blood cell analysis method, an analyzer and a storage medium, which can improve the accuracy of the white blood cell counting and classifying results of different types of animals.
The embodiment of the invention provides an animal blood cell analysis method, which is applied to an animal blood cell analyzer and comprises the following steps:
selecting a target measurement mode from a plurality of animal measurement modes, and determining a target dosage of the diluent according to the target measurement mode;
mixing the target dose of diluent with the blood of the animal to be detected and performing hemolysis treatment to obtain a detection sample;
and testing the detection sample to obtain a white blood cell counting and classifying result.
The embodiment of the invention provides an animal blood cell analyzer, which comprises:
the sampling needle assembly is used for sucking blood of an animal to be detected and sending the blood to the first counting cell assembly;
the mode selection module is used for selecting a target measurement mode from a plurality of animal measurement modes and determining the target dosage of the diluent according to the target measurement mode;
the reagent conveying assembly is used for mixing the target dosage of diluent with the blood of the animal to be detected and performing hemolysis treatment to obtain a detection sample;
and the first counting cell component is used for testing the detection sample to obtain a white blood cell counting and classifying result.
An embodiment of the present invention provides a storage medium, wherein the storage medium stores an executable program, and when the executable program is executed by a processor, the steps of the animal blood cell analysis method executed by the animal blood cell analyzer are realized.
In the embodiment of the invention, the target dosage of the diluent for diluting the animal blood is determined based on the animal measurement mode corresponding to the animal type, and the blood of the animal to be tested is diluted by the diluent with the target dosage, so that when the blood of the animal to be tested is tested, the blood of the animal to be tested is diluted by the diluent with the corresponding dosage for the blood of different types of animals, and the accuracy of the test result of leucocytes such as lymphocytes is improved by controlling the dosage of the diluent.
FIG. 1 is a schematic diagram of an alternative animal blood cell analyzer architecture provided by an embodiment of the present invention;
FIG. 2 is a schematic diagram illustrating the principle of an impedance counting test according to an embodiment of the present invention;
FIG. 3 is a schematic illustration of a distribution curve of white blood cells according to an embodiment of the present invention;
FIG. 4 is a schematic flow chart of an alternative method for analyzing animal blood cells according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of an alternative animal blood cell analyzer architecture provided by embodiments of the present invention;
FIG. 6 is a schematic flow chart of an alternative method for analyzing animal blood cells according to an embodiment of the present invention.
The present invention will be described in further detail below with reference to the accompanying drawings and examples. It should be understood that the examples provided herein are merely illustrative of the invention and are not intended to limit the invention. In addition, the following embodiments are provided as partial embodiments for implementing the present invention, not all embodiments for implementing the present invention, and the technical solutions described in the embodiments of the present invention may be implemented in any combination without conflict.
In various embodiments of the invention: the animal blood cell analyzer selects a target measurement mode from a plurality of animal measurement modes, and determines a target dosage of diluent according to the target measurement mode; mixing the target dose of diluent with the blood of the animal to be detected and performing hemolysis treatment to obtain a detection sample; and testing the detection sample to obtain a white blood cell counting and classifying result.
An embodiment of the present invention provides an animal blood cell analysis method, which is applied to an animal blood cell analyzer, as shown in fig. 1, the animal blood cell analyzer 100 includes: a sampling needle assembly 101, a mode selection module 102, a reagent delivery assembly 103, a first counting cell assembly 104. The sampling needle assembly 101 can comprise a sampling needle and a syringe, and is used for sucking blood of an animal to be tested and pumping the sucked blood of the animal to be tested into the first counting cell assembly; the mode selection module 102 is implemented by a processor in the animal blood cell analyzer for determining a current animal measurement mode. The reagent conveying assembly 103 is used for controlling the amount and time of reagents such as diluent and hemolytic agent entering the first counting cell assembly of the animal blood cell analyzer, and may include a syringe and a liquid path, wherein the liquid path is disposed between the storage location for storing the diluent and hemolytic agent and the first counting cell assembly, and is used for transferring the reagents such as diluent and hemolytic agent. The first counting cell assembly 104 is capable of performing a white blood cell sorting and counting test on blood samples of different species of animals in the first counting cell assembly.
The blood sample includes white blood cells, red blood cells, platelets, and the like, and can be obtained by treating whole blood drawn from an animal with a diluent, a hemolytic agent, and the like. The diluent is an isotonic solution having an acid-base buffering action and appropriate ionic strength and conductivity, and for example, the diluent contains hypoxanthine or xanthine compound or salt thereof as a main component, or may be other diluents capable of playing the role. The hemolytic agent is used for lysing the red blood cells to classify and count the white blood cells. The hemolytic agent includes a surfactant, which may specifically include a cationic surfactant and a nonionic surfactant. The amount and concentration of the hemolytic agent may be selected reasonably according to actual sample preparation requirements, and are not specifically limited herein. The hemolytic agent may be a quaternary ammonium salt ionic surfactant as a main component, or may be any other surfactant capable of exerting the above-described effects.
Here, the sampling needle sends the blood of the animal to be tested into the first counting cell assembly, the reagent conveying assembly adds the diluent and the hemolytic agent into the first counting cell assembly to obtain a blood sample, namely a detection sample, and the first counting cell assembly detects the detection sample, namely the blood sample, in the first counting cell assembly.
The counting and sorting of leukocytes may include three-sorting, four-sorting and five-sorting. The triage is an example of classifying leukocytes into three major groups, namely, a small cell group (a cell group consisting of lymphocytes), an intermediate cell group (a cell group consisting of monocytes) and a large cell group (a fine cell group consisting of granulocytes) are separately prepared from a fixed diluent, and the numbers of lymphocytes, monocytes and granulocytes in a blood sample are obtained. The five classifications can directly classify the white blood cells into neutrophils, lymphocytes, eosinophils, basophils and monocytes by means of certain dilution and chemical staining or impedance methods.
The first counting cell assembly can perform a white blood cell test on a blood sample of an animal by an impedance counting test. The procedure of the method of the first cuvette assembly for performing an impedance method counting test on a test sample in the first cuvette assembly may be as follows: the sampling needle sucks out quantitative animal blood to be detected from a blood sample, the animal blood is injected into the first counting cell assembly and is uniformly mixed with quantitative diluent to form diluent test solution with a certain dilution ratio, hemolytic agent is added, and impedance counting test is carried out in the first counting cell assembly after uniform mixing, so that a leukocyte channel counting result is obtained. The method comprises the steps of firstly diluting in a front pool of a first counting cell assembly to obtain a detection sample, and then leading the front pool to a rear pool of the first counting cell assembly through a detection hole under the action of negative pressure. In the counting process, the diluted sample flows into the rear pool from the front pool to form a stable flow field. Since a constant current source is applied between the positive electrode and the negative electrode, a stable electric field is formed as shown in fig. 2. Pulse signals are formed when blood cells pass through the detection pores 142, and particle division and counting are performed by the pulse signals.
According to the principle of an impedance method, after being treated by a hemolytic agent, red blood cells are ruptured, the volume of broken red blood cell fragments does not influence the counting of a white blood cell result, the white blood cells are reduced in volume under the action of the hemolytic agent, when the white blood cells with different volumes pass through small holes, the pulse sizes are different, and the white blood cells with the volume of 35-250 Fisher (fl) are treated. After treatment with a hemolytic agent, lymphocytes are in the small cell area; granulocytes are in the large cell region and monocytes are in the intermediate cell region. The animal blood cell analyzer calculates the percentage of each subgroup of cells according to the proportion of each subgroup in the total amount. In fig. 3, the white blood cell classification is shown schematically, GOHST is red blood cell debris, LYM is lymphocytes, MON is monocytes, and GRAN is neutrophils.
In the embodiment of the present invention, the first counting cell assembly does not have any limitation on the classifying number of the white blood cells of the detection sample.
Of course, the embodiments of the present invention are not limited to being provided as methods and hardware, and various implementations are possible, such as providing a storage medium (storing a program or instructions for executing the animal blood cell analysis method provided by the embodiments of the present invention).
Fig. 4 is a schematic flow chart of an implementation of the method for analyzing animal blood cells according to the embodiment of the present invention, as shown in fig. 4, including:
s401, selecting a target measurement mode from a plurality of animal measurement modes, and determining a target dosage of the diluent according to the target measurement mode.
A mode selection module in an animal blood cell analyzer provides a plurality of animal measurement modes, such as: rat measurement mode, cat measurement mode, dog measurement mode, etc., further for example, animal measurement mode 1, animal measurement mode 2, etc. In one example, an animal measurement mode corresponds to a species of animal, such as: the animal type corresponding to the rat measurement mode is rat, the animal type corresponding to the cat measurement mode is cat, and the animal type corresponding to the dog measurement mode is dog. In yet another example, one animal measurement mode corresponds to multiple species of animals, such as: the animal species corresponding to the animal measurement mode 1 include: mouse and cat, animal measurement mode 2 corresponds to animal species including: a dog.
One animal measurement mode has a corresponding dose of diluent. The dosage of the diluent corresponding to different animal measurement modes can be the same or different, that is, the dosage of the diluent corresponding to different animals can be the same or different.
In practical applications, the animal species with the same diluent dosage can correspond to the same animal measurement mode, and the animal species with different diluent dosages can correspond to different animal measurement modes. Such as: the animal species include: class A, class B, class C, and class D. The dosage of the diluent corresponding to the type a and the type C is dosage 1, the dosage of the diluent corresponding to the type B is dosage 2, and the dosage of the diluent corresponding to the type D is dosage 3, then the animal measurement mode corresponding to the type a and the type C is animal measurement mode a, the animal measurement mode corresponding to the type B is animal measurement mode B, the animal measurement mode corresponding to the type D is animal measurement mode C, the dosage of the diluent corresponding to the animal measurement mode a is dosage 1, the dosage of the diluent corresponding to the animal measurement mode B is dosage 2, and the dosage of the diluent corresponding to the animal measurement mode C is dosage 3.
The mode selection module in the animal blood cell analyzer determines an animal measurement mode corresponding to the blood of the animal to be tested based on the operation of a user or the scanning of a scanner in the animal blood cell analyzer, and takes the dosage of the diluent corresponding to the determined animal measurement mode as the current target dosage so as to determine the dosage of the diluent which needs to be injected into the first counting cell assembly currently.
S402, mixing the diluent with the target dose with the blood of the animal to be detected, and performing hemolysis treatment to obtain a detection sample.
After a mode selection module in the animal blood cell analyzer determines the target dosage of the diluent, the determined target is calculated and sent to a reagent conveying assembly, the reagent conveying assembly mixes the diluent with the target dosage with the blood of the animal to be detected, hemolysis treatment is carried out, and the detection sample is obtained. Here, the sampling needle assembly is used for sucking blood of the animal to be tested and sending the blood to the first counting cell assembly.
The reagent conveying assembly mixes the hemolytic agent with the blood of the animal to be detected so as to perform hemolytic treatment on the blood of the animal to be detected, dissolve red blood cells in the blood of the animal to be detected and retain white blood cells in the blood of the animal to be detected.
The sampling needle assembly sends the blood of the animal to be detected into the first counting cell assembly, the reagent conveying assembly adds the diluent with the target dose and the hemolytic agent into the first counting cell assembly, and the blood of the animal to be detected is diluted and subjected to hemolytic treatment in the first counting cell assembly to obtain a detection sample. Optionally, the reagent delivery assembly adds a target dose of diluent to the first count cell assembly at one time; optionally, the reagent delivery assembly dispenses a target dose of diluent into the first counting cell assembly.
In one example, the animal blood cell analyzer is provided with a diluent storage area for storing diluent and a hemolytic agent storage area for storing hemolytic agent, wherein the diluent storage area and the hemolytic agent storage area are respectively provided with a liquid path connected with the first counting pool assembly, and the reagent conveying assembly controls the flow of the diluent in the diluent storage area and the hemolytic agent in the hemolytic agent storage area to the first counting pool assembly and stops the flow of the diluent in the diluent storage area and the hemolytic agent in the hemolytic agent storage area by controlling the opening and closing of the corresponding liquid paths.
In an example, the animal blood cell analyzer is provided with a sampling needle for sucking a certain amount of blood of an animal to be tested from a test tube containing the blood of the animal to be tested, after the sampling needle sucks a certain amount of blood of the animal to be tested from the test tube containing the blood of the animal to be tested under the control of an injector, the sampling needle moves to a detection area where the first counting cell component is located, the injector pumps the blood of the animal to be tested in the sampling needle into the first counting cell component, and therefore the blood of the animal to be tested is added into the first counting cell component. Here, the sampling needle may be moved to above the first counting chamber assembly, before the injector injects the blood of the animal to be tested in the sampling needle into the first counting chamber assembly, the sampling needle may be moved to the inside of the first counting chamber assembly from above the first counting chamber assembly in the vertical direction, and the injector injects the blood of the animal to be tested in the sampling needle located inside the chamber into the first counting chamber assembly, thereby preventing the blood of the animal to be tested in the sampling needle from splashing out of the first counting chamber assembly.
The amount of the animal sample to be measured sucked by the sampling needle can be the same or different for different target measurement modes. In practical application, the same animal blood cell analyzer, the sampling needle absorbs the same dosage of the animal sample to be tested from the test tube, that is, the dosage of the animal blood to be tested added into the first counting cell component is the same, so that the test results of different types of animals can be compared conveniently.
In one embodiment of the present invention, the reagent delivery assembly may include one or more syringes, and the syringe for adding the diluent and the hemolysis agent to the first cuvette assembly may be one syringe or different syringes. Such as: the reagent delivery assembly includes a syringe through which the diluent and hemolysing agent are added to the first counting cell assembly, respectively. For another example: the reagent delivery assembly includes a first syringe for adding diluent to the first cuvette assembly and a second syringe. The second syringe is used to add a hemolysing agent to the first counting cell assembly. The number of syringes included in the reagent delivery assembly and the target of action of the syringes are not limited in any way in the embodiments of the present invention.
In the embodiment of the invention, the order of the sampling needle assembly for sending the blood of the animal to be tested into the first counting cell assembly and the reagent conveying assembly for adding the diluent and the hemolytic agent into the first counting cell assembly is not limited. Such as: the sampling needle assembly and the reagent conveying assembly sequentially add diluent, the blood of the animal to be tested and hemolytic agent into the first counting pool assembly. For another example: the sampling needle assembly and the reagent conveying assembly sequentially add diluent, hemolytic agent and blood of the animal to be tested into the first counting cell assembly. For another example: the sampling needle assembly and the reagent conveying assembly simultaneously add the diluent and the blood of the animal to be tested into the first counting pool assembly, and after the diluent and the blood of the animal to be tested are added, the reagent conveying assembly adds the hemolytic agent into the first counting pool assembly.
In practical application, the diluent is added while the blood of the animal to be detected is added, and the sampling needle for sucking the blood of the animal to be detected is cleaned through the diluent, so that the blood of the animal to be detected sucked in the sampling needle can be completely added into the first counting cell assembly.
In an embodiment, before the target dosage of the diluent is mixed with the blood of the animal to be tested and subjected to hemolysis treatment, the reagent conveying assembly of the animal blood cell analyzer evacuates the first counting cell assembly, so that the liquid in the first counting cell assembly is completely removed, and the influence of the residual liquid in the first counting cell assembly on the test result of the test is avoided.
In an embodiment, in the process of mixing the target dosage of diluent with the blood of the animal to be tested and performing hemolysis treatment, when the first counting cell component includes at least two of the diluent, the blood of the animal to be tested and the hemolytic agent, the reagent conveying component of the animal blood cell analyzer uniformly mixes the liquid in the first counting cell component, so that the diluent, the blood of the animal to be tested, the hemolytic agent and the blood of the animal to be tested are fully reacted, and the accuracy of the test result is ensured.
In the embodiment of the present invention, the amount of the hemolytic agent added by the reagent delivery assembly to the first counting chamber assembly can be adjusted according to the animal measurement mode, and the time for the hemolysis treatment, i.e. the reaction time between the hemolytic agent and the blood of the animal to be tested, can also be adjusted according to the animal measurement mode.
And S403, testing the detection sample to obtain a white blood cell counting and classifying result.
The sampling needle assembly sends the blood of the animal to be detected into the first counting cell assembly, the reagent conveying assembly adds the diluent and the hemolytic agent with target dose into the first counting cell assembly to obtain a detection sample after dilution and hemolysis treatment, and the detection result in the animal blood cell analyzer tests the detection sample in the first counting cell assembly to obtain the counting and classifying result of the leucocytes.
Optionally, after obtaining the test sample in the first cuvette assembly, the animal blood cell analyzer triggers testing of the test sample by the first cuvette assembly based on user manipulation. Optionally, the animal blood cell analyzer automatically triggers testing of the test sample by the first cuvette assembly after the test sample is obtained in the first cuvette assembly. In one example, the animal blood cell analyzer automatically triggers the testing of the test sample by the first cuvette assembly based on the reaction time of the hemolytic agent and the blood of the test animal.
After the first counting cell assembly obtains the white blood cell counting and classifying result, the white blood cell counting and classifying result can be sent to a display of the animal blood cell analyzer and presented to a detection person through the display of the animal blood cell analyzer, and the white blood cell counting and classifying result can also be sent to displays of other devices except the animal blood cell analyzer and presented to the detection person through the displays of the other devices. Here, the animal blood cell analyzer and other devices may be connected by wire, wireless, or the like.
In the embodiment of the invention, the target dosage of the diluent for diluting the animal blood is determined based on the animal measurement mode corresponding to the animal type, and the blood of the animal to be tested is diluted by the diluent with the target dosage, so that when the blood of the animal to be tested is tested, the blood of the animal to be tested is diluted by the diluent with the corresponding dosage for the blood of different types of animals, and the accuracy of the test result of leucocytes such as lymphocytes is improved by controlling the dosage of the diluent.
In the embodiment of the invention, for different types of animals, the dilution and hemolysis treatment of the blood of the animal to be tested can be adjusted by adjusting the dosage of the diluent, the dosage of the hemolytic agent and the reaction time of the hemolytic agent and the blood of the animal to be tested, so as to improve the accuracy of the leukocyte classification and counting results of the blood of various animals.
Based on the animal blood cell analyzer shown in fig. 1, the animal blood cell analyzer provided in the embodiment of the present invention can also include, as shown in fig. 5: a display 105 and an input device 106. The display 105 is used for displaying a software interface, and the input device 106 includes a keyboard or a mouse and other devices for a user to input information or instructions. In the embodiment of the present invention, the display 105 and the input device 106 may be integrated as a touch display, and receive information or instructions input by a user while displaying a software interface.
In one embodiment, based on the animal blood cell analyzer shown in fig. 5, S401 selects a target measurement mode from a plurality of animal measurement modes, including: outputting an animal measurement mode selection interface; receiving an input operation or a selection operation for an animal measurement mode in the measurement mode selection interface; determining a target measurement mode from a plurality of animal measurement modes according to the input operation or the selection operation.
Here, a display 105 for outputting an animal measurement mode selection interface; an input device 106 for receiving an input operation or a selection operation for an animal measurement mode in the measurement mode selection interface; a mode selection module 102 for determining a target measurement mode from a plurality of animal measurement modes according to the input operation or the selection operation.
The display can output an animal measurement mode selection interface used for selecting an animal measurement mode, a plurality of animal measurement modes or animal types can be displayed in the animal measurement mode, so that a user inputs the type or target measurement mode of the current animal to be measured through the input device based on the animal measurement mode selection interface, and when the information input by the user is the type of the animal, the mode selection module can determine the animal measurement mode corresponding to the input type of the animal from the animal measurement modes as the target measurement mode.
The input device may receive an input operation or a selection operation by a user. In the case where the input device receives an input operation of a user, an input interface may be provided in the animal measuring mode selection interface, through which the animal kind or the target measuring mode input by the user is received. In the case where the input device receives an input operation of a user, a plurality of animal species or animal measurement modes may be provided in the animal measurement mode selection interface, and the animal species or target measurement mode selected by the user may be determined.
Based on the animal blood cell analyzer shown in fig. 1, the animal blood cell analyzer provided in the embodiment of the present invention can also include, as shown in fig. 5: a scanner 107. The scanner 107 is configured to scan information identifiers such as a barcode and a two-dimensional code, and obtain information carried by the information identifiers.
In one embodiment, based on the animal blood cell analyzer shown in fig. 5, a target measurement mode is selected from a plurality of animal measurement modes, including: scanning an information identifier on a test tube containing the blood of the animal to be detected to obtain sample information of the blood of the animal to be detected; and selecting a target measuring mode from a plurality of animal measuring modes according to the sample information.
Here, the scanner 107 is configured to scan an information identifier on a test tube containing blood of the animal to be tested, so as to obtain sample information of the blood of the animal to be tested; and the mode selection module 102 is used for selecting a target measurement mode from a plurality of animal measurement modes according to the sample information.
Hold the test tube of awaiting measuring animal blood and paste to have the label, and have information sign such as bar code, the two-dimensional code that carries the information of awaiting measuring animal blood on the label, the information that the information sign carried can include: the type of the animal to which the blood of the animal to be tested belongs, a Universal Unique Identifier (UUID) of the blood of the animal to be tested, a test item and the like. The sample information carried by the information identifier is not limited in any way in the embodiment of the invention.
When a test tube containing animal blood to be measured is located in the scanning range of a scanner, the scanner scans an information identifier on the test tube to obtain sample information of the animal blood to be measured carried by the information identifier, and sends the scanned sample information of the animal blood to be measured to a mode selection module, and the mode selection module determines an animal measurement mode, namely a target measurement mode, corresponding to the animal blood to be measured in the test tube according to sample information related to the animal type or the animal measurement mode in the sample information scanned by the scanner.
Such as: the sample information scanned by the scanner comprises information representing an animal measurement mode, and if the animal measurement mode is a cat measurement mode, the mode selection module determines that the target measurement mode is the cat measurement mode. For another example: the sample information scanned by the scanner comprises the animal type to which the blood of the animal to be detected belongs, and if the animal type is a cat, the model selection module determines that the target measurement mode is the cat measurement mode. For another example, the sample information scanned by the scanner includes a sample ID, i.e., UUID, of the blood of the animal to be measured, the sample identifier is 1111, the mode selection module determines that the animal measurement mode corresponding to the blood of the animal to be measured is the cat measurement mode according to the sample identifier 1111, and then determines that the target measurement mode is the cat measurement mode.
In the embodiment of the present invention, based on whether the target dose of the diluent is added to the first counting cell assembly at one time, the manner of adding the diluent, the blood of the animal to be tested and the hemolytic agent to the first counting cell assembly in the embodiment of the present invention may include:
adding mode one, adding at one time
In the first addition mode, S402 mixes the target dose of the diluent with blood of an animal to be tested and performs hemolysis treatment to obtain a test sample, including: adding a target dosage of diluent to the first counting cell assembly; and adding a hemolytic agent and the blood of the animal to be detected into the first counting pool component to obtain a detection sample. Here, the sampling needle assembly sends blood of an animal to be tested into a first counting cell assembly, and the reagent conveying assembly adds a target dosage of diluent into the first counting cell assembly; and adding a hemolytic agent to the first counting well assembly.
When the reagent conveying assembly adds the diluent to the first counting well assembly, the target dosage of the diluent is directly added to the first counting well assembly, and after the target dosage of the diluent is added, the hemolytic agent is added to the first counting well assembly. The sampling needle can send the blood of the animal to be tested into the first counting cell assembly during or after the reagent conveying assembly adds the diluent into the first counting cell assembly. Such as: the reagent conveying component adds a target dosage of diluent into the first counting pool component, the sampling needle component adds blood of an animal to be tested into the first counting pool component after the reagent conveying component adds the target dosage of diluent, and the reagent conveying component continues to add hemolytic agent into the first counting pool component after the sampling needle component adds the blood of the animal to be tested. For another example: in the process of adding the reagent conveying assembly into the first counting pool assembly with the target dosage of the diluent, the sampling needle assembly adds the blood of the animal to be tested into the first counting pool assembly, and after the sampling needle assembly adds the blood of the animal to be tested into the first counting pool assembly, the reagent conveying assembly continues to add the hemolytic agent into the first counting pool assembly.
In the embodiment of the invention, in the case of adding the target dose of the diluent at one time, the process of adding the diluent is uninterrupted, but the sequence of adding the diluent, the hemolytic agent and the blood of the animal to be tested is not limited at all.
Adding mode two, adding in portions
In the first addition mode, S402, mixing the target dose of the diluent with blood of the animal to be tested and performing hemolysis treatment, including: adding the blood of the animal to be detected into the diluent with the first dose to obtain a reference detection sample; adding a second dose of diluent and hemolytic agent to the reference test sample to obtain the test sample, wherein the sum of the first dose and the second dose is the target dose. Here, the reagent delivery assembly adds a first dose of diluent to the first counting chamber assembly before or while the sampling needle assembly feeds the blood of the test animal into the first counting chamber; and adding a second dose of diluent and the hemolytic agent into the first counting pool assembly after adding the blood of the animal to be tested into the first counting pool assembly.
The reagent conveying assembly firstly adds a first dose of diluent into the first counting cell assembly, the sampling needle assembly adds the blood of the animal to be detected into the first counting cell assembly, after the blood of the animal to be detected is added into the first counting cell assembly by the sampling needle assembly, the reagent conveying assembly continuously adds a second dose of diluent into the first counting cell assembly, and adds a hemolytic agent, so that the first dose of diluent is firstly added into the first counting cell assembly, and then the second dose of diluent is added into the first counting cell assembly.
Here, the addition of the first dose of diluent to the first counting chamber assembly by the reagent delivery assembly may be performed prior to the addition of the blood of the test animal to the first counting chamber assembly by the sampling needle assembly, or may be performed simultaneously with the addition of the blood of the test animal to the first counting chamber assembly by the sampling needle assembly.
Under the condition that the dosages of the blood of the animal to be tested added in different animal measurement modes are the same, the first dosage can be the same or different.
In one embodiment, the animal blood cell analyzer aspirates a fixed dose of the reference test sample from the reference test sample prior to adding a second dose of the diluent and the hemolytic agent to the reference test sample; performing a red blood cell test and/or a platelet test on the fixed dose of the reference test sample. Here, a sampling needle assembly in the animal blood cell analyzer sucks a fixed dose of a reference test sample composed of the first dose of the diluent and the blood of the test animal from the first cuvette assembly; the second counting cell assembly is used for carrying out a red blood cell test and/or a platelet test on the fixed dose of the reference detection sample.
The second counting cell component is a different counting cell component than the first counting cell component. Optionally, the second counting cell assembly and the first counting cell assembly belong to the same animal blood cell analyzer. Optionally, the second counting cell assembly and the first counting cell assembly belong to different animal blood cell analyzers.
Before the hemolytic agent is added into the first counting cell assembly to form the detection sample, the first counting cell assembly only comprises a first dosage of diluent and blood of the animal to be detected, namely, a reference detection sample for diluting the blood of the animal to be detected in the first counting cell assembly, at this time, the reference detection sample can be sucked from the first counting cell assembly by the sampling needle assembly, and the reference detection sample sucked by the sampling needle can be subjected to at least one of the following tests related to red blood cells by the second counting cell assembly: red blood cell tests and platelet tests.
The dose of the reference test sample drawn from the first cuvette assembly by the sampling needle assembly, i.e. the fixed dose, may be 9uL, 11uL, etc. The size of the fixed dose can be set according to actual requirements, and the embodiment of the present invention is not limited in any way.
In one embodiment, the first dose is the same for different animal measurement modes.
For different animal measurement modes, namely different animal types, the dosage of the animal sample to be detected in the reference detection sample is the same, and under the condition that the dosage of the diluent is the same, the dilution ratios of the reference detection samples corresponding to the different animal types are the same, so that the comparison of the erythrocyte detection or platelet detection results of the different animal types is facilitated.
In the second addition mode, based on whether the first dose of diluent is added to the first counting chamber assembly at one time, the second addition mode can include the following two cases:
case one, the first dose of diluent is added at one time
In a first case, the adding the blood of the test animal to the first dose of the diluent to obtain a reference test sample includes: adding a first dose of diluent to the first counting cell assembly; and adding the blood of the animal to be detected into the first counting cell assembly to obtain a reference detection sample. Here, the reagent delivery assembly adds a first dose of diluent to the first counting cell assembly prior to the sampling needle assembly delivering the blood of the test animal to the first counting cell assembly.
Case two, the first dose of diluent was added in portions
In case two, the adding the blood of the animal to be tested to the first dose of the diluent to obtain a reference test sample includes: adding a third dose of diluent to the first counting cell assembly; and adding a fourth dose of diluent and the blood of the animal to be detected into the first counting cell assembly to obtain a reference detection sample, wherein the sum of the third dose and the fourth dose is the first dose. Here, the reagent delivery assembly adds a third dose of diluent to the first counting cell assembly prior to the sampling needle assembly delivering the blood of the test animal to the first counting cell assembly; and adding a fourth dose of diluent and the blood of the animal to be tested into the first counting assembly when the blood of the animal to be tested is sent into the first counting assembly by the sampling needle assembly.
It should be noted that, the first case and the second case are examples of adding the target amount of the diluent to the first counting chamber assembly twice and adding the target amount of the diluent to the first counting chamber assembly three times, respectively, and in practical applications, the number of times of adding the target amount of the diluent to the first counting chamber assembly, and the adding sequence of the hemolyzing agent and the blood to be tested may be controlled according to practical requirements.
The reagent conveying assembly and the sampling needle assembly can simultaneously add fourth dosage of diluent and the blood of the animal to be detected into the first counting cell assembly, the blood of the animal to be detected on the sampling needle is washed by the added diluent, the blood of the animal to be detected can be completely added into the first counting cell assembly, and the accuracy of classification and counting results is guaranteed.
According to the animal blood cell analysis method provided by the embodiment of the invention, an operator can flexibly set a target dosage of diluent adding process according to actual requirements, such as: when the target dosage of diluent is added, the first dosage of diluent and the blood of the animal to be detected are added into the first counting cell assembly, and then the second dosage of diluent and the hemolytic agent are added into the first counting cell assembly, so that a reference detection sample is obtained in the process of forming the detection sample, and the tests related to red blood cells, such as a red blood cell test, a platelet test and the like, can be carried out while the white blood cell test is carried out.
In the following, the method for analyzing animal blood cells provided by the embodiment of the present invention is described by using a specific test scenario, as shown in fig. 6, including:
s601, the sampling needle assembly sucks blood of an animal to be detected.
The sampling needle assembly draws a prescribed dose of blood from the test tube.
And S602, emptying the first counting cell assembly.
S603, adding a first dosage of diluent into the first counting chamber assembly by the reagent conveying assembly.
And the injector in the reagent conveying assembly opens a liquid path between the first counting cell assembly and the diluent storage position, diluent is added into the first counting cell assembly through the opened liquid path, and the opened liquid path is closed after the diluent with the first dosage is added. In the process, the liquid path between the first counting cell assembly and the hemolytic agent storage position is in a closed state.
And S604, adding the blood of the animal to be tested into the first counting cell assembly by the sampling needle assembly.
The injector in the sampling needle assembly controls the sampling needle to move from a sample suction area for sucking the blood of the animal to be tested from the test tube to the upper part of the first counting cell assembly in a detection area for detecting the blood sample and move from the upper part of the first counting cell assembly into the pool of the first counting cell assembly, and the injector in the sampling needle assembly pumps the blood of the animal to be tested in the sampling needle in the pool of the first counting cell assembly into the first counting cell assembly.
And S605, uniformly mixing.
And uniformly mixing the diluent in the first counting cell component and the blood of the animal to be detected to obtain a reference detection sample.
And S606, the sampling needle assembly sucks the reference detection sample from the first counting cell assembly to perform red blood cell testing and platelet testing.
S607, the reagent conveying assembly adds hemolytic agent to the first counting well assembly.
And a syringe in the reagent conveying assembly opens a liquid path between the first counting pool assembly and the hemolytic agent storage position, hemolytic agent is added into the first counting pool assembly through the opened liquid path, and the opened liquid path is closed after the hemolytic agent is added. In the process, a liquid path between the first counting cell assembly and the diluent storage position is in a closed state.
And S608, adding a second dosage of diluent into the first counting cell assembly by the reagent conveying assembly.
The reagent delivery assembly adds diluent to the first counting chamber assembly as in S603.
And S609, uniformly mixing.
And uniformly mixing the diluent, the animal blood to be detected and the hemolytic agent in the first counting cell assembly to obtain a detection sample.
S610, testing the first counting cell assembly.
The first counting cell assembly tests the reference sample in the first counting cell assembly to obtain a white blood cell counting and classifying result.
According to the animal blood cell analysis method provided by the embodiment of the invention, when the leukocyte channel test is carried out among different animal species, different diluent dosage is used among different animals, and the accuracy of the lymphocyte test result is provided on the premise of ensuring the accuracy of the leukocyte count value test result by adjusting the hemolytic dose and hemolytic time.
Before the animal blood cell analysis method provided by the embodiment of the invention is not adopted, the percentage of lymphocytes in the blood sample of the mouse is 65%, the blood sample is subjected to microscopic examination, the percentage of lymphocytes in the microscopic examination result is 75%, and the microscopic examination result is 10% different from the actual measurement result. We found, when tested: the same problem exists in not one sample but almost all samples, and the lymphocyte count can be improved by adjusting the adding amount of different hemolytic agents and increasing the reaction time, but the problem of inaccurate lymphocyte count cannot be completely solved. According to the animal blood cell analysis method provided by the embodiment of the invention, the accuracy of a lymphocyte test result can be further improved by changing the amount of the diluent.
The results of the animal blood cell analysis method provided in the examples of the present invention will be described below with reference to the results of counting lymphocytes in blood samples of different kinds of animals.
In this embodiment, the amount of blood collected may be 9 ul. All measurement modes corresponded to 2.4ml of dilution before adjustment. Table 1 blood of 7 horses was collected to form blood of 7 test animals, and the amount of diluent after adjustment was set to 1.4ml, and the percentage of lymphocytes before the amount of diluent was adjusted, the percentage of lymphocytes after the amount of diluent was adjusted, and the percentage of lymphocytes in microscopic examination results were compared. Table 2 blood of 7 mice was collected to form blood of 7 test animals, and the amount of diluent after adjustment was set to 1.6ml, and the percentage of lymphocytes before the amount of diluent was adjusted, the percentage of lymphocytes after the amount of diluent was adjusted, and the percentage of lymphocytes in microscopic examination results were compared. Wherein Lym% represents the percentage of lymphocytes.
TABLE 1,
TABLE 2,
As can be seen from tables 1 and 2, the accuracy of the lymphocyte test result can be ensured on the basis of ensuring the accuracy of the white blood cell counting result by adjusting the dosage of the blood sample diluent of different animals in the white blood cell counting.
An embodiment of the present invention further provides a storage medium, that is, a computer-readable storage medium, where the storage medium stores an executable program, and the executable program, when executed by a processor, implements the steps of the animal blood cell analysis method executed by the animal blood cell analyzer.
The above description of the media embodiment is similar to the description of the method embodiment described above, with similar beneficial effects as the method embodiment. For technical details not disclosed in the embodiments of the storage medium according to the invention, reference is made to the description of the embodiments of the method according to the invention.
In the embodiment of the present invention, if the above-mentioned animal blood cell analysis method is implemented in the form of a software functional module and sold or used as a separate product, it may also be stored in a computer-readable storage medium. Based on such understanding, the technical solutions of the embodiments of the present invention may be essentially implemented or a part contributing to the prior art may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the methods described in the embodiments of the present invention. And the aforementioned storage medium includes: various media capable of storing program codes, such as a usb disk, a removable hard disk, a Read Only Memory (ROM), a magnetic disk, or an optical disk. Thus, embodiments of the invention are not limited to any specific combination of hardware and software.
It should be appreciated that reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of the phrases "in one embodiment" or "in an alternative embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. It should be understood that, in various embodiments of the present invention, the sequence numbers of the above-mentioned processes do not mean the execution sequence, and the execution sequence of each process should be determined by its function and inherent logic, and should not constitute any limitation on the implementation process of the embodiments of the present invention. The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
In the embodiments provided in the present invention, it should be understood that the disclosed apparatus and method may be implemented in other ways. The above-described device embodiments are merely illustrative, for example, the division of the unit is only a logical functional division, and there may be other division ways in actual implementation, such as: multiple units or components may be combined, or may be integrated into another system, or some features may be omitted, or not implemented. In addition, the coupling, direct coupling or communication connection between the components shown or discussed may be through some interfaces, and the indirect coupling or communication connection between the devices or units may be electrical, mechanical or other forms.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units; can be located in one place or distributed on a plurality of network units; some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, all the functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may be separately regarded as one unit, or two or more units may be integrated into one unit; the integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional unit.
Those of ordinary skill in the art will understand that: all or part of the steps for realizing the method embodiments can be completed by hardware related to program instructions, the program can be stored in a computer readable storage medium, and the program executes the steps comprising the method embodiments when executed; and the aforementioned storage medium includes: various media that can store program codes, such as a removable Memory device, a Read Only Memory (ROM), a magnetic disk, or an optical disk.
Alternatively, the integrated unit of the present invention may be stored in a computer-readable storage medium if it is implemented in the form of a software functional module and sold or used as a separate product. Based on such understanding, the technical solutions of the embodiments of the present invention may be essentially implemented or a part contributing to the prior art may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the methods described in the embodiments of the present invention. And the aforementioned storage medium includes: a removable storage device, a ROM, a magnetic or optical disk, or other various media that can store program code.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (19)
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| PCT/CN2019/106669 WO2021051349A1 (en) | 2019-09-19 | 2019-09-19 | Method for analysis of animal blood cells, analyzer, and storage medium |
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| CN113125785B (en) * | 2021-03-29 | 2024-02-27 | 深圳市科曼医疗设备有限公司 | Method for detecting high-concentration sample and invoking time sequence |
| EP4174488B1 (en) * | 2021-08-31 | 2025-07-23 | Shenzhen Mindray Animal Medical Technology Co., Ltd. | Specimen analysis apparatus and specimen analysis method |
| CN113777009B (en) * | 2021-08-31 | 2024-04-16 | 深圳迈瑞动物医疗科技股份有限公司 | Cell analyzer and cell detection method suitable for sheep, goats and cows |
| WO2024234317A1 (en) * | 2023-05-17 | 2024-11-21 | 深圳迈瑞动物医疗科技股份有限公司 | Blood analysis apparatus and method for species |
| CN116298348A (en) * | 2023-05-17 | 2023-06-23 | 深圳迈瑞动物医疗科技股份有限公司 | Blood analysis device and method for species |
| CN116359525B (en) * | 2023-06-01 | 2023-11-24 | 深圳市帝迈生物技术有限公司 | Blood analysis device and control method thereof |
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