CN103990503A - Disposable pipette coating tip and using method thereof - Google Patents
Disposable pipette coating tip and using method thereof Download PDFInfo
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- CN103990503A CN103990503A CN201410207441.9A CN201410207441A CN103990503A CN 103990503 A CN103990503 A CN 103990503A CN 201410207441 A CN201410207441 A CN 201410207441A CN 103990503 A CN103990503 A CN 103990503A
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- 238000000576 coating method Methods 0.000 title claims abstract description 55
- 239000011248 coating agent Substances 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 32
- 238000003780 insertion Methods 0.000 claims abstract description 4
- 230000037431 insertion Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 229920002635 polyurethane Polymers 0.000 claims description 4
- 239000004814 polyurethane Substances 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 229920001971 elastomer Polymers 0.000 claims description 3
- 238000004528 spin coating Methods 0.000 claims description 3
- 239000000853 adhesive Substances 0.000 claims description 2
- 230000001070 adhesive effect Effects 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
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Abstract
本发明涉及一种一次性移液枪涂布枪头及其使用方法，该枪头与移液枪配合使用，接口部、中间螺纹管、枪头本体。接口部、中间螺纹管、枪头本体三部分适于按照接口部、中间螺纹管、枪头本体的次序依次连接，接口部还适用于与移液器的插入部结合；该接口部为圆台形或圆柱形，表面为光滑表面；接口部、中间螺纹管、枪头本体其所使用的制作材质透明或者半透明；该中间螺纹管由一定韧性且可变形的材料组成，其材质是与接口部和枪头本体相一致，或者根据需要适用其它材质，其外径内径上下均一。本发明所涉及一次性涂布枪头具有移液和涂布两种功能，操作简便，可弯曲，耐高温，耐高压，一次性，省时省工，污染少，用量大，用户广。本发明还涉及一次性移液枪涂布枪头的使用方法和步骤。
The invention relates to a disposable pipette gun coating gun head and a use method thereof. The gun head is used in conjunction with the pipette gun, and comprises an interface part, an intermediate threaded tube, and a gun head body. The three parts of the interface, the intermediate threaded tube, and the tip body are suitable for sequential connection in the order of the interface, the intermediate threaded tube, and the tip body, and the interface is also suitable for combining with the insertion portion of the pipette; the interface is in the shape of a truncated cone Or cylindrical, the surface is a smooth surface; the material used for the interface, the intermediate threaded tube, and the gun head body is transparent or translucent; the intermediate threaded tube is composed of a certain toughness and deformable material, and its material is the same as that of the interface. It is consistent with the gun head body, or other materials can be used according to the needs, and its outer diameter and inner diameter are uniform up and down. The disposable coating tip of the present invention has two functions of pipetting and coating, and is easy to operate, bendable, high temperature resistant, high pressure resistant, disposable, saves time and labor, less pollution, large dosage and wide users. The present invention also relates to the use method and steps of the coating tip of the disposable pipette gun.
Description
技术领域 technical field
本发明属于生命科学/生物领域，涉及微生物学、分子生物学实验用具及使用的具体技术领域，尤其涉及一种用于实验液体涂布的一次性移液枪涂布枪头及其使用方法。 The invention belongs to the field of life science/biology, relates to the specific technical field of microbiology, molecular biology experiment equipment and use, and in particular relates to a disposable pipette gun coating tip for experimental liquid coating and a use method thereof.
背景技术 Background technique
在微生物学实验和分子生物学实验中，涂布棒的使用较为广泛，如常见的L型涂布棒，而且普通枪头也是频繁更换，用量极大。目前平板涂布用的是涂布棒，而涂布棒材料一般为是玻璃的或者不锈钢的。 In microbiology experiments and molecular biology experiments, coating rods are widely used, such as common L-shaped coating rods, and ordinary pipette tips are frequently replaced, and the amount used is extremely large. At present, coating rods are used for flat plate coating, and the materials of coating rods are generally glass or stainless steel.
目前这样的实验用具存在很大的缺陷：涂布棒本身弊端很多，玻璃涂布棒使用时必需在火焰的灼烧下，高温不易握持，而且容易打碎；不锈钢的涂布棒由于钢铁比热太小，升温后散热缓慢，无谓的等待时间过长，而且涂布棒均涂一次需要烧一次，用起来较为麻烦。本发明可以克服以上缺点，优点在于：1、枪头在移除了密封套后，可以弯曲，其初始状态可以具备普通枪头的移液功能，拉伸弯曲后可以用于涂布；2、枪头可以作为涂布棒，也可移液；3、枪头为塑料制品，一次性的，生产价格低廉，比涂布棒节约成本；4、可以高压灭菌；5、使用过程中不用火焰灼烧；6、一个枪头替代了以往的枪头和涂布棒，操作方便、简单快速，省时省力；7、具有一般枪头的移液枪功能；8、可减少遗撒溅出等污染；9、适用于液体提取涂布的各个领域。10、能够与标准移液枪枪配套使用,具有广泛的应用领域。 At present, such experimental equipment has great defects: the coating rod itself has many disadvantages, and the glass coating rod must be burned by the flame when it is used, and it is not easy to hold at high temperature, and it is easy to break; The heat is too small, the heat dissipation is slow after heating up, the unnecessary waiting time is too long, and the coating rod needs to be burned once for every coating, which is troublesome to use. The present invention can overcome the above disadvantages, and the advantages are: 1. The pipette head can be bent after the sealing sleeve is removed, and its initial state can have the liquid transfer function of a common pipette head, and can be used for coating after being stretched and bent; 2. The tip of the gun can be used as a coating rod, and can also be used for pipetting; 3. The tip is made of plastic, disposable, and the production price is low, which saves cost compared with the coating rod; 4. It can be sterilized by autoclaving; 5. No flame is used during use Burning; 6. One tip replaces the previous tip and coating rod, which is convenient, simple and fast to operate, saving time and effort; 7. It has the function of a pipette with a general tip; 8. It can reduce pollution such as spilling and splashing ; 9. Applicable to various fields of liquid extraction and coating. 10. It can be used with standard pipette guns and has a wide range of applications.
总之，一次性移液枪涂布枪头易于操作，省时省力，涂布效果好，既能移液也能涂布，无菌操作有保障。 In short, the disposable pipette gun coating tip is easy to operate, saves time and effort, and has a good coating effect. It can be used for both pipetting and coating, and the aseptic operation is guaranteed.
发明内容 Contents of the invention
本发明提出一种一次性移液枪涂布枪头，与移液枪配合使用，以及使用其的涂布方法： The present invention proposes a disposable pipette gun coating tip, used in conjunction with the pipette gun, and a coating method using it:
涂布枪头结构组成包括：接口部、中间螺纹管、枪头本体。 The structural composition of the coating gun head includes: the interface part, the intermediate threaded pipe, and the gun head body.
其接口部、中间螺纹管、枪头本体三部分适于按照接口部、中间螺纹管、枪头本体的次序依次连接，接口部还适用于与移液器的插入部结合；接口部的内径与一般标准移液器的内径相互配合；该接口部为圆台形或圆柱形，表面为光滑表面；为圆台形时，与移液器相接的口部口径较大。 Its three parts, the interface part, the middle threaded tube and the gun head body, are suitable to be connected in sequence according to the order of the interface part, the middle threaded tube, and the gun head body, and the interface part is also suitable for combining with the insertion part of the pipette; the inner diameter of the interface part and Generally, the inner diameters of standard pipettes match each other; the interface is frustum-shaped or cylindrical, with a smooth surface; when it is in the shape of a frustum, the diameter of the mouth connected to the pipette is larger.
该中间螺纹管由一定韧性且可变形的材料组成，其外径内径上下均一；其材质可以是聚氨酯类材料，如TPU等。 The intermediate threaded pipe is made of a tough and deformable material, and its outer diameter and inner diameter are uniform up and down; its material can be polyurethane material, such as TPU.
该枪头本体为锥形，出液口口径较细，与中间螺纹管连接处口径较粗，外表面光滑。 The gun head body is tapered, the diameter of the liquid outlet is relatively small, the diameter of the joint with the intermediate threaded pipe is relatively thick, and the outer surface is smooth.
接口部、中间螺纹管、枪头本体均由耐高压材料制成，其所使用的制作材质透明或者半透明；中间螺纹管的材质可以与接口部和枪头本体相一致；也可根据需要适用其它材质；中间螺纹管具有可简单去除的密封套，中间螺纹管初始呈紧密的螺纹全紧缩状态，同时外套密封套，去除密封套后可拉伸、缩回，并弯曲一定角度；密封套与中间螺纹管为同一材质，之间有多处片状连接，片状连接处均具有易于分离的开口，便于在普通的外力作用下使密封套与中间螺纹管分离；呈紧密的螺纹全紧缩状态的中坚螺纹管，其结构与一般枪头没有大的区别，可以承担普通移液任务等。 The interface, the intermediate threaded tube, and the gun head body are all made of high-pressure resistant materials, and the materials used are transparent or translucent; the material of the intermediate threaded tube can be consistent with the interface and the gun head body; it can also be applied as needed Other materials; the intermediate threaded pipe has a sealing sleeve that can be easily removed. The intermediate threaded pipe is initially in a state of tight thread full tightening. At the same time, the sealing sleeve is covered. The middle threaded pipe is made of the same material, and there are multiple sheet-shaped connections between them, and the sheet-shaped connections have openings that are easy to separate, which facilitates the separation of the sealing sleeve from the middle threaded pipe under the action of ordinary external force; it is in a state of tight thread full tightening The backbone of the threaded tube, its structure is not much different from the general tip, and can undertake common pipetting tasks.
接口部与中间螺纹管的连接，以及中间螺纹管与枪头本体的连接，可以是粘贴式密封连接，也可以是螺纹式密封连接，或带密封胶圈的螺纹式密封连接，在这里，本领域技术人员可以根据具体需要选择连接方式，如实验用液体、实验条件要求不高，则可以用粘贴式密封连接，如果对实验条件要求比较苛刻，则可以选择带密封胶圈的螺纹式密封连接；其用于接口部、中间螺纹管、枪头本体的材质为塑料，颜色为无色或者具备一定颜色。 The connection between the interface part and the middle threaded pipe, and the connection between the middle threaded pipe and the body of the gun head, can be a paste-type sealed connection, a threaded sealed connection, or a threaded sealed connection with a sealing rubber ring. Here, this Those skilled in the art can choose the connection method according to the specific needs. For example, the liquid used in the experiment and the experimental conditions are not demanding, you can use the adhesive seal connection. If the experimental conditions are more demanding, you can choose the threaded seal connection with a sealing rubber ring The material used for the interface part, the intermediate threaded pipe, and the gun head body is plastic, and the color is colorless or has a certain color.
其适合200μl 移液枪规格的枪头的一次性移液枪涂布枪头的具体参数为：接口部长度1-2cm，开口部内径5-6mm，外径7-8mm，厚度1-2mm，用于插入移液枪；中间螺纹管可伸缩弯曲90˚，长度5mm-1cm，内腔直径3-4mm，外径5-6mm；枪头本体：锥形长度3-4cm，锥形上部腔直径3-4mm，外径4-6mm，厚度1-2mm。 The specific parameters of the disposable pipette gun coating tip suitable for the tip of the 200μl pipette gun specification are: the length of the interface is 1-2cm, the inner diameter of the opening is 5-6mm, the outer diameter is 7-8mm, and the thickness is 1-2mm. Used to insert a pipette; the middle threaded tube can be stretched and bent by 90°, the length is 5mm-1cm, the inner diameter is 3-4mm, and the outer diameter is 5-6mm; the tip body: the tapered length is 3-4cm, the tapered upper chamber diameter 3-4mm, outer diameter 4-6mm, thickness 1-2mm.
其适合1000μl移液枪规格的枪头的一次性移液枪涂布枪头的具体参数：接口部长度1-2cm，开口部内径5-6mm，外径7-8mm，厚度1-2mm，用于插入移液枪；中间螺纹管可伸缩弯曲90˚，长度1-2cm，内腔直径4-5mm，外径6-7mm；枪头本体：锥形，长度4-6cm，锥形上部腔直径5-6mm，外径7-8mm，厚度1-2mm。 The specific parameters of the disposable pipette gun coating tip suitable for the tip of the 1000μl pipette gun specification: the length of the interface is 1-2cm, the inner diameter of the opening is 5-6mm, the outer diameter is 7-8mm, and the thickness is 1-2mm. For inserting a pipette; the middle threaded tube can be stretched and bent by 90°, the length is 1-2cm, the inner diameter is 4-5mm, and the outer diameter is 6-7mm; the tip body: tapered, the length is 4-6cm, the diameter of the upper chamber is tapered 5-6mm, outer diameter 7-8mm, thickness 1-2mm.
接口部、中间螺纹管、枪头本体的材质分别可以是PP、聚氨酯材料、PP，密封套可以是任何可以稍用力便方便去除的塑料材料。 The material of the interface part, the middle threaded pipe, and the gun head body can be PP, polyurethane material, and PP respectively, and the sealing sleeve can be any plastic material that can be easily removed with a little force.
利用了本发明的一次性移液枪涂布枪头的移液使用方法，包括以下步骤： Utilize the pipetting use method of disposable pipette gun coating tip of the present invention, comprise the following steps:
1）选取合适规格的移液枪，将移液枪插入接口部，保持紧密连接； 1) Select a pipette of appropriate specifications, insert the pipette into the interface, and keep it tightly connected;
2）用移液枪吸取样品液体至需要的量，移取液体量不超过移液枪规格所能够容纳的液体量； 2) Use the pipette gun to draw the sample liquid to the required amount, and the amount of liquid pipetted shall not exceed the amount of liquid that the pipette gun specification can hold;
3）利用枪头，将吸取液体滴到平皿培养基表面：这里滴出的液体量是适于涂布的量，具体根据检测的对象液体的不同、实验目的的不同而有所不同； 3) Using the tip of the pipette, drop the absorbed liquid onto the surface of the culture medium of the plate: the amount of liquid dripped here is suitable for coating, which varies according to the different liquids to be tested and the different experimental purposes;
4）将枪头本体尖端顶在平皿盖内面，让枪头中间螺纹部分弯曲，同时让枪倾斜，与培养基表面成一定角度，先前后推拉数次，然后再旋转涂布，这样可完成液体的均匀涂布； 4) Put the tip of the gun head against the inner surface of the petri dish cover, let the middle thread part of the gun head bend, and at the same time let the gun tilt to form a certain angle with the surface of the culture medium, push and pull several times before and after, and then rotate to coat, so that the liquid can be completed uniform coating;
5）涂布完成后，将移液枪枪头弃去； 5) After the coating is completed, discard the tip of the pipette;
步骤（4）中的操作具体为：将枪头本体尖端顶在平皿盖内面，让枪头中间螺纹部分弯曲90˚，同时让枪倾斜，与培养基表面成30-45°，先前后推拉2-3次，然后再旋转涂布，这样可完成菌液的均匀涂布；所选取的可以配合的移液枪规格可以是200μl或者1000μl的两种规格，也可以选取其他常见规格移液枪。如400、600、800ul等等。 The operation in step (4) is as follows: put the tip of the pipette body against the inner surface of the petri dish cover, bend the middle threaded part of the pipette head by 90°, and at the same time, tilt the pipette to form a 30-45° angle with the surface of the culture medium, push and pull 2 -3 times, and then spin coating, so that the uniform coating of the bacterial solution can be completed; the selected pipette specifications can be 200μl or 1000μl, and other common specifications can also be selected. Such as 400, 600, 800ul and so on.
附图说明： 具体见说明书摘要附图1。附图1一次性移液枪涂布枪头的结构组成。 1. 接口部 2. 中间螺纹管 3. 枪头本体。 Description of drawings: For details, please refer to attached drawing 1 of the abstract of the description. Accompanying drawing 1 is the structural composition of disposable pipette gun coating tip. 1. Interface part 2. Intermediate threaded tube 3. Gun head body.
具体实施方式：Detailed ways:
使用了一次性移液枪涂布枪头，其组成包括接口部、中间螺纹管、枪头本体。 A disposable pipette gun is used to coat the tip, which consists of an interface, an intermediate threaded tube, and the tip body.
接口部、中间螺纹管、枪头本体三部分适于按照接口部、中间螺纹管、枪头本体的次序依次连接，接口部还适用于与移液器的插入部结合。 The three parts of the interface part, the intermediate threaded tube and the gun head body are suitable to be connected in sequence according to the order of the interface part, the intermediate threaded tube and the gun head body, and the interface part is also suitable for combining with the insertion part of the pipette.
该接口部为圆柱形，表面为光滑表面；该中间螺纹管由一定韧性且可变形的材料组成，其外径内径上下均一。 The interface is cylindrical with a smooth surface; the intermediate threaded pipe is made of a tough and deformable material, and its outer diameter and inner diameter are uniform up and down.
该枪头本体为锥形，出液口口径较细，与中间螺纹管连接处口径较粗，外表面光滑。 The gun head body is tapered, the diameter of the liquid outlet is relatively small, the diameter of the joint with the intermediate threaded pipe is relatively thick, and the outer surface is smooth.
接口部、中间螺纹管、枪头本体均由耐高压材料制成，其所使用的制作材质透明或者半透明。中间螺纹管的材质可以与接口部和枪头本体相一致；也可根据需要适用其它材质。 The interface part, the intermediate threaded pipe, and the gun head body are all made of high-pressure resistant materials, and the materials used are transparent or translucent. The material of the intermediate threaded pipe can be consistent with the interface part and the body of the gun head; other materials can also be applied as required.
中间螺纹管具有可简单去除的密封套，中间螺纹管初始呈紧密的螺纹全紧缩状态，同时外套密封套，去除密封套后可拉伸、缩回，并弯曲一定角度。 The intermediate threaded tube has a sealing sleeve that can be easily removed. The intermediate threaded tube is initially in a state of tight threads and fully tightened. At the same time, the sealing sleeve is covered. After the sealing sleeve is removed, it can be stretched, retracted, and bent at a certain angle.
接口部与中间螺纹管的连接，以及中间螺纹管与枪头本体的连接，是粘贴式密封连接。 The connection between the interface part and the middle threaded pipe, and the connection between the middle threaded pipe and the body of the gun head are pasting-type sealed connections.
用于接口部、中间螺纹管、枪头本体的材质为塑料，颜色为乳白色。 The material used for the interface part, the intermediate threaded pipe, and the body of the gun head is plastic, and the color is milky white.
该枪头为适合200μl 移液枪规格的枪头的一次性移液枪涂布枪头，具体参数： The pipette tip is a disposable pipette gun coating tip suitable for the pipette tip of 200μl pipette gun specification. Specific parameters:
接口部长度1-2cm，开口部内径5-6mm，外径7-8mm，厚度1-2mm，用于插入移液枪；中间螺纹管可伸缩弯曲90˚，长度5mm-1cm，内腔直径3-4mm，外径5-6mm；枪头本体：锥形长度3-4cm，锥形上部腔直径3-4mm，外径4-6mm，厚度1-2mm。 The length of the interface is 1-2cm, the inner diameter of the opening is 5-6mm, the outer diameter is 7-8mm, and the thickness is 1-2mm, which is used to insert the pipette; the middle threaded tube can be stretched and bent by 90°, the length is 5mm-1cm, and the inner diameter is 3mm -4mm, outer diameter 5-6mm; tip body: tapered length 3-4cm, tapered upper cavity diameter 3-4mm, outer diameter 4-6mm, thickness 1-2mm.
接口部、中间螺纹管、枪头本体的材质分别是PP、聚氨酯材料、PP。 The material of the interface part, the intermediate threaded tube, and the gun head body are PP, polyurethane material, and PP respectively.
一次性移液枪涂布枪头的其使用方法，包括以下步骤①选取所需枪头，将移液枪插入上部接口部，保持紧密连接； ②用移液枪吸取样品液体至需要的量，移取液体量不超过200μl；③将吸取液体滴到平皿培养基表面：④将枪头本体尖端顶在平皿盖内面，让枪头中间螺纹管弯曲90˚，同时让枪倾斜，与培养基表面成45°，先前后推拉3次，然后再旋转涂布，这样可完成菌液的均匀涂布。⑤涂布完成后，将移液枪枪头弃去。 The method of using the tip of the disposable pipette gun coating includes the following steps: ①Select the required tip, insert the pipette into the upper interface, and keep it tightly connected; ②Use the pipette to draw the sample liquid to the required amount, Pipette no more than 200μl of liquid; ③Drip the absorbed liquid onto the surface of the culture medium in the plate: ④Put the tip of the pipette body against the inner surface of the plate cover, let the middle threaded tube of the pipette bend 90˚, and at the same time let the gun tilt to meet the surface of the culture medium At 45°, push and pull 3 times before and after, and then spin coating, so that the uniform coating of the bacterial solution can be completed. ⑤ After the coating is completed, discard the tip of the pipette.
通过使用结果表明：本发明所涉及一次性涂布枪头具有移液和涂布两种功能，操作简便，可弯曲，耐高温，耐高压，一次性，省时省工，污染少，用量大，用户广。 The result of use shows that the disposable coating gun head involved in the present invention has two functions of pipetting and coating, easy to operate, bendable, high temperature resistance, high pressure resistance, disposable, time saving and labor saving, less pollution, and large consumption , a wide range of users.
以上所述用以说明本发明的技术方案，而非对其限制；尽管参照前述内容对本发明进行了详细的说明，本领域的普通技术人员应当理解：其依然可以对前述所记载的技术方案和相关内容进行修改，或者对其中部分技术特征进行等同替换；而这些修改或者替换，并不使相应技术方案的本质脱离本发明实施例技术方案的精神和范围。 The above description is used to illustrate the technical solution of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the aforementioned content, those of ordinary skill in the art should understand that: it can still understand the technical solution and the technical solution described above. Relevant content is modified, or some technical features are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiment of the present invention.
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| CN103990503B (en) | 2015-09-09 |
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