CN103999857A - Application of tetrachloro bisphenol A as antibacterial agent and antibacterial material - Google Patents
Application of tetrachloro bisphenol A as antibacterial agent and antibacterial material Download PDFInfo
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- CN103999857A CN103999857A CN201410178888.8A CN201410178888A CN103999857A CN 103999857 A CN103999857 A CN 103999857A CN 201410178888 A CN201410178888 A CN 201410178888A CN 103999857 A CN103999857 A CN 103999857A
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- antibacterial
- tetrachlorobisphenol
- antibacterial agent
- tetrachloro bisphenol
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- KYPYTERUKNKOLP-UHFFFAOYSA-N Tetrachlorobisphenol A Chemical compound C=1C(Cl)=C(O)C(Cl)=CC=1C(C)(C)C1=CC(Cl)=C(O)C(Cl)=C1 KYPYTERUKNKOLP-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 38
- 239000000463 material Substances 0.000 title claims abstract description 27
- 239000003242 anti bacterial agent Substances 0.000 title claims abstract description 21
- 241000192125 Firmicutes Species 0.000 claims abstract description 11
- 229920003023 plastic Polymers 0.000 claims description 7
- -1 polypropylene Polymers 0.000 claims description 6
- 239000003822 epoxy resin Substances 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 229920000647 polyepoxide Polymers 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- KXGFMDJXCMQABM-UHFFFAOYSA-N 2-methoxy-6-methylphenol Chemical compound [CH]OC1=CC=CC([CH])=C1O KXGFMDJXCMQABM-UHFFFAOYSA-N 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 229920001568 phenolic resin Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000005007 epoxy-phenolic resin Substances 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 9
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 4
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 3
- 241000187747 Streptomyces Species 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 6
- RNFJDJUURJAICM-UHFFFAOYSA-N 2,2,4,4,6,6-hexaphenoxy-1,3,5-triaza-2$l^{5},4$l^{5},6$l^{5}-triphosphacyclohexa-1,3,5-triene Chemical compound N=1P(OC=2C=CC=CC=2)(OC=2C=CC=CC=2)=NP(OC=2C=CC=CC=2)(OC=2C=CC=CC=2)=NP=1(OC=1C=CC=CC=1)OC1=CC=CC=C1 RNFJDJUURJAICM-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000003063 flame retardant Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000088 plastic resin Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 239000006391 Luria-Bertani Medium Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005011 phenolic resin Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
Description
技术领域technical field
本发明涉及抗菌剂领域，特别涉及一种四氯双酚A作为抗菌剂的应用及抗菌材料。The invention relates to the field of antibacterial agents, in particular to an application of tetrachlorobisphenol A as an antibacterial agent and an antibacterial material.
背景技术Background technique
抗菌材料是一类具有杀菌、抑菌性能的新型功能材料，应用广泛。其核心成分是抗菌剂，抗菌剂主要分为两大类：无机抗菌剂和有机抗菌剂，通过将少量抗菌剂添加到普通材料中，使之具有抗菌性能。Antibacterial materials are a new class of functional materials with bactericidal and antibacterial properties, and are widely used. Its core component is antibacterial agent, and antibacterial agent is mainly divided into two categories: inorganic antibacterial agent and organic antibacterial agent, by adding a small amount of antibacterial agent to ordinary materials, it has antibacterial properties.
抗菌材料主要分为无机抗菌材料和有机抗菌材料。无机抗菌材料虽然活性高，但易变色、成本较高；而有机抗菌剂由于耐热性差，不适于添加入塑料、树脂，这些都限制了抗菌材料的使用范围。Antibacterial materials are mainly divided into inorganic antibacterial materials and organic antibacterial materials. Although inorganic antibacterial materials have high activity, they are prone to discoloration and high cost; organic antibacterial agents are not suitable for adding to plastics and resins due to their poor heat resistance, which limits the scope of application of antibacterial materials.
四氯双酚A(Tetrachlorobisphenol A，TCBPA)是一种阻燃剂，可作为反应型阻燃剂，添加于环氧树脂中，也可作为添加剂型阻燃剂，添加于抗冲击聚苯乙烯和酚醛树脂中。由于四氯双酚A对化学纤维的良好阻燃效果，使得该阻燃剂用途不断扩大。Tetrachlorobisphenol A (Tetrachlorobisphenol A, TCBPA) is a kind of flame retardant, which can be used as a reactive flame retardant, added to epoxy resin, and can also be used as an additive flame retardant, added to impact-resistant polystyrene and In phenolic resin. Due to the good flame retardant effect of tetrachlorobisphenol A on chemical fibers, the use of this flame retardant is continuously expanding.
发明内容Contents of the invention
为了解决现有技术的问题，本发明实施例提供了一种四氯双酚A作为抗菌剂的应用及抗菌材料。所述技术方案如下：In order to solve the problems in the prior art, an embodiment of the present invention provides an application of tetrachlorobisphenol A as an antibacterial agent and an antibacterial material. Described technical scheme is as follows:
一方面，本发明实施例提供了一种四氯双酚A作为抗菌剂的应用，所述四氯双酚A适用于革兰氏阳性菌的抗菌剂，所述四氯双酚A的结构式为：On the one hand, the embodiment of the present invention provides an application of tetrachlorobisphenol A as an antibacterial agent, the tetrachlorobisphenol A is suitable for the antibacterial agent of Gram-positive bacteria, and the structural formula of the tetrachlorobisphenol A is: :
另一方面，本发明实施例提供了一种抗菌材料，所述抗菌材料包括塑料和树脂中的一种、以及四氯双酚A，且所述四氯双酚A的质量分数为5％-20％。On the other hand, an embodiment of the present invention provides an antibacterial material, the antibacterial material includes one of plastic and resin, and tetrachlorobisphenol A, and the mass fraction of tetrachlorobisphenol A is 5%- 20%.
具体地，所述塑料为聚丙烯、聚乙烯或聚苯乙烯。Specifically, the plastic is polypropylene, polyethylene or polystyrene.
具体地，所述树脂为环氧树脂或酚醛树脂。Specifically, the resin is epoxy resin or phenolic resin.
本发明实施例提供的技术方案带来的有益效果是：本发明提供的四氯双酚A能够显著抑制革兰氏阳性菌的生长，对金黄色葡萄球菌、芽胞杆菌和链霉菌等的最小抑制浓度为2-8μg/ml，可见该四氯双酚A的抗菌效果良好，是良好的抗菌剂，本发明提供的抗菌材料包括四氯双酚A，通过添加四氯双酚A可使抗菌率达99％以上，使得该抗菌材料对革兰氏阳性菌的生长能够起到良好的抑制作用。The beneficial effects brought by the technical solutions provided by the embodiments of the present invention are: tetrachlorobisphenol A provided by the present invention can significantly inhibit the growth of Gram-positive bacteria, and has minimal inhibition on Staphylococcus aureus, Bacillus and Streptomyces, etc. Concentration is 2-8 μ g/ml, it can be seen that the antibacterial effect of this tetrachlorobisphenol A is good, is good antibacterial agent, the antibacterial material provided by the present invention comprises tetrachlorobisphenol A, can make antibacterial rate by adding tetrachlorobisphenol A Reaching more than 99%, the antibacterial material can play a good inhibitory effect on the growth of Gram-positive bacteria.
具体实施方式Detailed ways
为使本发明的目的、技术方案和优点更加清楚，下面将对本发明实施方式作进一步地详细描述。In order to make the purpose, technical solution and advantages of the present invention clearer, the implementation manners of the present invention will be further described in detail below.
实施例一Embodiment one
将四氯双酚A进行最小抑制浓度(Minimum Inhibition Concentration，MIC)测试实验，具体实验步骤如下：Tetrachlorobisphenol A is carried out minimum inhibitory concentration (Minimum Inhibition Concentration, MIC) test experiment, concrete experiment procedure is as follows:
从平板上接种少许菌于装有LB培养基(Luria-Bertani培养基)的锥形瓶中，37℃摇床过夜培养12h-14h；其中，菌包括：革兰氏阳性菌和革兰氏阴性菌；LB培养基的配方如下:胰蛋白胨(Tryptone)10g/L，酵母提取物(Yeast extract)5g/L，氯化钠(NaCl)10g/L。Inoculate a little bacteria from the plate in an Erlenmeyer flask equipped with LB medium (Luria-Bertani medium), and culture overnight on a shaker at 37°C for 12h-14h; among them, the bacteria include: Gram-positive bacteria and Gram-negative bacteria Bacteria; The formula of LB medium is as follows: tryptone (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium chloride (NaCl) 10g/L.
第2天上午从锥形瓶中吸取菌液以1％的体积比转接于装有5ml的LB培养基的锥形瓶中，继续于37℃摇床中培养4h-5h，至OD600(OD600表示该菌液在600nm波长处的吸光值)为0.6～0.8，并记录菌液的具体OD值(optical density，光密度)。In the morning of the second day, the bacterium solution was drawn from the Erlenmeyer flask and transferred to an Erlenmeyer flask equipped with 5 ml of LB medium at a volume ratio of 1%, and continued to cultivate in a shaker at 37°C for 4h-5h until OD600 (OD600 Indicates that the absorbance value of the bacterial liquid at a wavelength of 600nm) is 0.6 to 0.8, and the specific OD value (optical density, optical density) of the bacterial liquid is recorded.
用LB培养基将培养后的菌液稀释1000倍，使菌液浓度为105CFU/ml，吸取稀释后的菌液，并加至96孔板中，每孔150μl。The cultured bacterial solution was diluted 1000 times with LB medium to make the concentration of the bacterial solution 10 5 CFU/ml. The diluted bacterial solution was pipetted and added to a 96-well plate, 150 μl per well.
配制浓度为128μg/ml的四氯双酚A溶液，吸取150μl该四氯双酚A溶液加至96孔板中的第1孔中，混匀后吸出150μl至第2孔中，以此类推。Prepare a tetrachlorobisphenol A solution with a concentration of 128 μg/ml, pipette 150 μl of the tetrachlorobisphenol A solution into the first well of a 96-well plate, mix well and pipette 150 μl into the second well, and so on.
滴加完毕后，将96孔板放入摇床中经37℃培养12h-14h。After the dropwise addition, the 96-well plate was placed in a shaker and incubated at 37°C for 12h-14h.
肉眼观察，没有变浑浊的孔所对应的最小的浓度即为该菌的最小抑制浓度，具体参见表1。Visually, the minimum concentration corresponding to the wells that do not become turbid is the minimum inhibitory concentration of the bacteria, see Table 1 for details.
表1为四氯双酚A对几种细菌的最小抑制浓度(MIC)Table 1 is the minimum inhibitory concentration (MIC) of tetrachlorobisphenol A to several bacteria
由表1可见，四氯双酚A对革兰氏阳性菌具有良好的抑制效果。It can be seen from Table 1 that tetrachlorobisphenol A has a good inhibitory effect on Gram-positive bacteria.
本发明提供的四氯双酚A能够显著抑制革兰氏阳性菌的生长，对金黄色葡萄球菌、芽胞杆菌和链霉菌等的最小抑制浓度为2-8μg/ml，可见该四氯双酚A的抗菌效果良好，是良好的抗菌剂。Tetrachlorobisphenol A provided by the present invention can significantly inhibit the growth of Gram-positive bacteria, and the minimum inhibitory concentration to Staphylococcus aureus, Bacillus and Streptomyces etc. is 2-8 μ g/ml, it can be seen that tetrachlorobisphenol A It has good antibacterial effect and is a good antibacterial agent.
实施例二Embodiment two
按照中华人民共和国国家标准“家用和类似用途电器的抗菌、除菌、净化功能抗菌材料的特殊要求”中使用的方法，将四氯双酚A分别添加于聚丙烯塑料和环氧树脂中制备成抗菌材料，并对该抗菌材料进行抗菌测试，其中，四氯双酚A的质量分数可以为5％、10％和20％。According to the method used in the National Standard of the People's Republic of China "Special Requirements for Antibacterial, Sterile and Purifying Antibacterial Materials for Household and Similar Electrical Appliances", tetrachlorobisphenol A is added to polypropylene plastic and epoxy resin to prepare antibacterial material, and carry out antibacterial test on this antibacterial material, wherein, the mass fraction of tetrachlorobisphenol A can be 5%, 10% and 20%.
试验结果显示在聚丙烯塑料和环氧树脂中的四氯双酚A对金黄色葡萄球菌的抗菌率均达99％以上。The test results show that tetrachlorobisphenol A in polypropylene plastic and epoxy resin has an antibacterial rate of more than 99% against Staphylococcus aureus.
本发明提供的抗菌材料包括四氯双酚A，通过添加四氯双酚A可使抗菌率达99％以上，使得该抗菌材料对革兰氏阳性菌的生长能够起到良好的抑制作用。The antibacterial material provided by the invention includes tetrachlorobisphenol A, and the antibacterial rate can reach more than 99% by adding tetrachlorobisphenol A, so that the antibacterial material can play a good inhibitory effect on the growth of Gram-positive bacteria.
以上所述仅为本发明的较佳实施例，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the present invention. within range.
Claims (4)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410178888.8A CN103999857A (en) | 2014-04-29 | 2014-04-29 | Application of tetrachloro bisphenol A as antibacterial agent and antibacterial material |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410178888.8A CN103999857A (en) | 2014-04-29 | 2014-04-29 | Application of tetrachloro bisphenol A as antibacterial agent and antibacterial material |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410178888.8A Pending CN103999857A (en) | 2014-04-29 | 2014-04-29 | Application of tetrachloro bisphenol A as antibacterial agent and antibacterial material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107897181A (en) * | 2017-12-08 | 2018-04-13 | 江汉大学 | A kind of application of bisphenol-A and anti-biotic material |
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| US3651128A (en) * | 1968-06-11 | 1972-03-21 | Givaudan Corp | Bacteriostatic carbamates of bisphenols |
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| US5846215A (en) * | 1997-12-11 | 1998-12-08 | Chesebrough-Pond's Usa Co., Division Of Conopco, Inc. | Antibacterial swabs |
| CN1380370A (en) * | 2002-03-25 | 2002-11-20 | 葛明槽 | Fire-resistant powder coating material and its production process |
| US20060167115A1 (en) * | 2003-01-06 | 2006-07-27 | Yossef Gohary | Compositions for the preservation of timber |
| CN102558807A (en) * | 2010-12-27 | 2012-07-11 | 重庆工商大学 | Formula of glass fiber reinforced flame-retardant antistatic polycarbonate (PC) plastic for oil treatment |
-
2014
- 2014-04-29 CN CN201410178888.8A patent/CN103999857A/en active Pending
Patent Citations (6)
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|---|---|---|---|---|
| US3651128A (en) * | 1968-06-11 | 1972-03-21 | Givaudan Corp | Bacteriostatic carbamates of bisphenols |
| US3860705A (en) * | 1971-11-08 | 1975-01-14 | Armour Pharma | Antimicrobial compositions containing aluminum halide compounds |
| US5846215A (en) * | 1997-12-11 | 1998-12-08 | Chesebrough-Pond's Usa Co., Division Of Conopco, Inc. | Antibacterial swabs |
| CN1380370A (en) * | 2002-03-25 | 2002-11-20 | 葛明槽 | Fire-resistant powder coating material and its production process |
| US20060167115A1 (en) * | 2003-01-06 | 2006-07-27 | Yossef Gohary | Compositions for the preservation of timber |
| CN102558807A (en) * | 2010-12-27 | 2012-07-11 | 重庆工商大学 | Formula of glass fiber reinforced flame-retardant antistatic polycarbonate (PC) plastic for oil treatment |
Non-Patent Citations (2)
| Title |
|---|
| OKAZAKI, KANZO; ET AL.: "Chemotherapeutics for dermatomycoses. V. Fungistatic activity of bis- and triphenols", 《YAKUGAKU ZASSHI》, vol. 72, no. 11, 31 December 1952 (1952-12-31), pages 1403 - 1404 * |
| 张亨: "几种重要的有机氯系阻燃剂", 《中国氯碱》, no. 07, 31 July 2002 (2002-07-31), pages 25 - 27 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107897181A (en) * | 2017-12-08 | 2018-04-13 | 江汉大学 | A kind of application of bisphenol-A and anti-biotic material |
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