CN103875657A - Uvioresistant farm-oriented water dispersible granule containing natamycin and preparation method of water dispersible granule - Google Patents

Abstract

The invention belongs to the field of farm-oriented antibacterial agents, and specifically discloses an uvioresistant farm-oriented water dispersible granule containing natamycin and a preparation method of the water dispersible granule. The water dispersible granule comprises the following components in percentage by weight: 10%-30% of a natamycin slow release agent, 1%-10% of a dispersing agent, 20%-45% of a wetting agent, 0.5%-5% of a binding agent, 1%-20% of a disintegrating agent and 20%-40% of blue-green algae powder. The uvioresistant farm-oriented water dispersible granule containing the natamycin, which is provided by the invention, has the characteristics that the uvioresistant farm-oriented water dispersible granule is green and environment-friendly, efficient and stable, long in lasting period, safe to use. resistant to ultraviolet rays and the like; the uvioresistant farm-oriented water dispersible granule containing natamycin is used for fields such as field planting, long-distance transportation, storage preservation and the like of agricultural products.

Description

A kind of anti-ultraviolet agricultural water dispersible granule containing natamycin and its preparation method

technical field

The invention relates to an agricultural antibacterial agent, in particular to an anti-ultraviolet agricultural water dispersible granule containing natamycin which has protective effects on the planting, transportation and storage of agricultural products and has good photostability and a preparation method thereof.

Background technique

Water-dispersible granules are a new formulation of pesticides developed in the 1980s. Because of their good safety, no organic solvents, no dust, and convenient packaging, transportation, and use, they have been rapidly developed. As an important development direction of new pesticide environmental protection formulations, water dispersible granules have become the main formulations of pesticides at home and abroad in recent years.

Natamycin (Natamycin) is a natural antifungal compound produced by Streptomyces fermentation. It belongs to polyene macrolides. It relies on its lactone ring structure to interact with sterol compounds on the fungal cell membrane to form antibiotic-sterol compounds. , thereby disrupting the structure of the fungal cytoplasmic membrane. The hydrophilic part (polyol part) of the macrolide forms water holes on the membrane, which damages the permeability of the cell membrane, which in turn causes the leakage of amino acids, electrolytes and other substances in the bacteria, and the bacteria die. When there is no sterol compound on the cell membrane of certain microorganisms, natamycin has no effect on it, so natamycin can not only widely and effectively inhibit the growth of various molds and yeasts, but also inhibit the production of mycotoxins. At the same time, natamycin is a biological antibacterial product with good biological safety. It has been officially approved by the China Food Additive Standard Chemical Technology Committee. Natamycin can be used as a food preservative. It is currently widely used in food preservation and antifungal. treat.

Fungal diseases are the largest category of plant diseases, accounting for about 70-80% of plant diseases. Many plant diseases with serious damage and wide distribution are caused by fungi. In 1970, the great spot disease of corn in the United States was prevalent, which reduced the corn production by 16.5 billion kilograms; in 1980, the small spot disease was prevalent, which reduced the corn production by 1.7 billion kilograms; in 1974, the Asian rice blast epidemic caused a loss of 6 billion kilograms of rice production. In addition, wheat rust, corn smut, potato late blight, and cotton wilt are also fungal diseases. As a high-efficiency green antifungal substance, natamycin has not been widely promoted and applied in agricultural production. One of the important reasons is that it has a ring chemical structure, is sensitive to ultraviolet rays, and is easy to decompose during field use. Inactivation, the duration of drug effect is not long, so it limits its promotion and application in the agricultural field as a green high-efficiency antibacterial agent.

Contents of the invention

In order to overcome the deficiencies of the prior art above, the present invention provides a natamycin-containing anti-ultraviolet agricultural water dispersible granule and a preparation method thereof. It can solve the shortcomings of natamycin, which is easy to decompose when exposed to light and reduce biological activity during the application process in the agricultural field, and prolong the use period. And processed into water dispersible granules, no organic solvents are used, the active ingredient content is as high as 10-50%, the dispersion and suspension stability are good, and it has slow-release effect, the duration of effect can reach 25-30 days, and the suspension rate can reach 90% % or more, the fineness of dispersed particles reaches 1-20 microns, and the medicinal effect and commercial performance are good.

The present invention is achieved through the following technical solutions:

An anti-ultraviolet agricultural water dispersible granule containing natamycin, which is composed of the following components in weight percentage: 10-30% of natamycin slow-release agent, 1-10% of dispersant, and 20-10% of wetting agent 45%, binder 0.5-5%, disintegrant 1-20%, blue-green algae powder 20-40%.

Water-dispersible granules are generally composed of active ingredients, wetting agents, dispersants, disintegrants, stabilizers, binders and carriers, etc., with a particle size of 200-5000um, and can quickly disintegrate and disperse after entering water to form a high-suspension dispersion system . Usually, the content of wetting agent in water-dispersible granules is 2-8%; however, the content of wetting agent in water-dispersible granules for natamycin in the invention is as high as 20-45%, preferably 20-30%.

The ingredients of the natamycin slow-release agent include: 20-35% natamycin, 60-80% blue-green algae powder and 1-2% carbomer resin. The preparation method of described natamycin slow-release agent, comprises the following steps:

S1. Prepare natamycin methanol solution at room temperature at 25°C, add dry cyanobacteria powder, oscillate and soak in an air bath oscillator at a constant temperature of 25-30°C for 20-30 hours, rotary evaporate, and dry to prepare natamycin- Spirulina powder. the

S2. Natamycin-cyanobacteria powder was added to the magnetically stirred carbomer resin alcohol solution, and the solvent was removed by rotary evaporation, and the natamycin sustained-release agent was prepared by jet crushing.

The dispersant includes one or more mixtures of T-2700, NNO, T-2020, KY-555, and SHQ-1.

The wetting agent includes fatty alcohol sulfate, alkyl sulfate, alkyl alcohol polyoxyethylene ether sulfate sodium, alkylphenol polyoxyethylene ether sodium sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate Sulfates, Sodium Alkyl Sulfonates, Alkyl Benzene Sulfonates, Alkyl Naphthalene Sulfonates, Alkyl Succinate Sulfonates, Sodium Monoalkylphenyl Polyoxyethylene Succinate Sulfonates, Fatty One or more of alcohol ethylene oxide adduct sulfonate, alkylphenol formaldehyde condensate ethylene oxide adduct sulfonate, alkanamide taurate, T-1004, K12 mix.

The binder includes one or more mixtures of xanthan gum, carboxymethyl cellulose, soluble starch, bentonite and polyethylene glycol.

The disintegrating agent includes one or more mixtures of ammonium sulfate, sodium sulfate, sodium chloride, urea, and soluble starch.

 The preparation method of the above-mentioned anti-ultraviolet agricultural water dispersible granule containing natamycin is carried out in the following steps:

S1. Prepare natamycin methanol solution at room temperature at 25°C, add dry cyanobacteria powder, oscillate and soak in an air bath oscillator at a constant temperature of 25-30°C for 20-30 hours, rotary evaporate, and dry to prepare natamycin- Spirulina powder.

S2. Natamycin-cyanobacteria powder was added to the magnetically stirred carbomer resin alcohol solution, and the solvent was removed by rotary evaporation, and the natamycin sustained-release agent was prepared by jet crushing.

S3. According to the formula ratio mentioned above, after stirring and mixing natamycin slow-release agent, dispersant, wetting agent, binder, disintegrating agent, and blue-green algae powder, pulverize to obtain 500～800 mesh fine powder, add to Add an appropriate amount of water to the fine powder, mix well, extrude to granulate, dry, and sieve to obtain water-dispersible granules.

The beneficial effects of the present invention are:

Since the 1980s, water-dispersible granules have gradually become an important development direction of new pesticide environmental protection formulations. A large number of water-dispersible granules for different active ingredients have also been disclosed in the prior art, but they are different from general water-dispersible granules. Compared, in the process of preparing the anti-ultraviolet agricultural water dispersible granule containing natamycin, in order to overcome the problem that natamycin is easy to decompose and fail under ultraviolet rays, the present invention is used to protect natamycin by adding cyanobacteria powder, and Adding carbomer resin, the effective use period is extended to 25-30 days.

The present invention disperses natamycin in water with micronized particles, and has the following characteristics: 1. The product cost is not high, and it is easy to pack and transport. 2. Water-based, no organic solvent, no dust, less environmental pollution, safe for production and users. 3. After the preparation is sprayed, it is evenly dispersed on the surface of crops and products with fine particles. It has good antibacterial effect, is not easy to be decomposed by light, and has a long antibacterial cycle.

Detailed ways

The present invention will be further described in detail below in conjunction with specific examples. Unless otherwise specified, the reagents and equipment used in the present invention are conventional reagents and equipment in the technical field.

Example 1

  Firstly, weigh the various components for the preparation of natamycin sustained release agent: 20% natamycin, 79% cyanobacteria powder and 1% carbomer resin. The preparation of natamycin sustained-release preparation follows the following steps: 1) Prepare natamycin methanol solution at room temperature at 25°C, add dry cyanobacteria powder, shake and soak in an air bath oscillator at a constant temperature of 25-30°C for 20-30 hours , rotary evaporation, and drying to prepare natamycin-cyanobacteria powder. 2) Natamycin-cyanobacteria powder was added to the magnetically stirred carbomer resin alcohol solution, the solvent was removed by rotary evaporation, and the natamycin sustained-release agent was prepared by jet crushing.

Next, weigh and prepare each component of the anti-ultraviolet agricultural water dispersible granule containing natamycin: 10% natamycin sustained-release agent, 5% dispersant (T-2700), wetting agent (T-1004) 30%, binder xanthan gum 2%, disintegrant sodium sulfate 13%, blue-green algae powder 40%. Above-mentioned raw material is prepared according to the following steps:

Stir and mix natamycin sustained-release agent, dispersant, wetting agent, binder, disintegrant, and blue-green algae powder according to the above proportions, then pulverize to obtain 500-800 mesh fine powder, and add appropriate amount of water to the fine powder, After mixing, extrude to granulate, dry and sieve to obtain water-dispersible granules.

Example 2

First weigh the various components for preparing the natamycin sustained-release agent: 30% natamycin, 68% cyanobacteria powder and 2% carbomer resin. The preparation of natamycin sustained-release preparation follows the following steps: 1) Prepare natamycin methanol solution at room temperature at 25°C, add dry cyanobacteria powder, shake and soak in an air bath oscillator at a constant temperature of 25-30°C for 20-30 hours , rotary evaporation, and drying to make natamycin-cyanobacteria powder. 2) Natamycin-cyanobacteria powder was added to the magnetically stirred carbomer resin alcohol solution, the solvent was removed by rotary evaporation, and the natamycin sustained-release agent was prepared by jet crushing.

Next, weigh and prepare each component of the anti-ultraviolet agricultural water dispersible granule containing natamycin: Natamycin sustained-release agent 20%, dispersant NNO 5%, wetting agent (K12) 25%, binding agent Bentonite 5%, disintegrant sodium chloride 15%, filler cyanobacteria powder 30%. The above raw materials are prepared according to the following steps: after stirring and mixing natamycin slow-release agent, dispersant, wetting agent, binder, disintegrating agent, and blue-green algae powder according to the above ratio, pulverize to obtain 500-800 mesh fine powder, Add an appropriate amount of water to the fine powder, mix well, extrude to granulate, dry, and sieve to obtain water-dispersible granules.

Measure the performance of the anti-ultraviolet agricultural water dispersible granules containing natamycin described in Examples 1-2, the results are as follows: the water dispersible granules prepared by the present invention do not use organic solvents, and the active ingredient content is as high as 10-50 %, good dispersion and suspension stability, and has slow-release effect, the duration of effect can reach 25-30 days, the suspension rate can reach more than 90%, the particle size after dispersion can reach 1-20 microns, the efficacy and commercial performance good. Moreover, after 4 hours of ultraviolet light irradiation, the natamycin content of the water-dispersible granules prepared in Examples 1-2 was only reduced by 20%, while that of the control sample (natamycin) was reduced by 50%. the

Claims (8)

1. containing a uvioresistant agricultural water dispersible granule for natamycin, it is characterized in that, formed by the component of following percentage by weight: natamycin sustained release agent 10～30%, dispersant 1～10%, wetting agent 20～45%, binding agent 0.5～5%, disintegrant 1～20%, blue algae powder 20～40%.

2. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, is characterized in that, described natamycin sustained release agent composition comprises: 20～35% natamycins, 60～80% blue algae powders and 1～2% carbomer resin.

3. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1 and 2, is characterized in that, the preparation method of natamycin sustained release agent is:

Under S1.25 DEG C of room temperature, prepare natamycin methanol solution, add dry blue algae powder, in the gas bath oscillator under 25～30 DEG C of constant temperature, vibration is soaked 20～30 hours, and rotary evaporation is dry, prepares natamycin-blue algae powder;

S2. natamycin-blue algae powder is added in the carbomer resin alcoholic solution of magnetic agitation, rotary evaporation, except desolventizing, through air-flow crushing, makes natamycin sustained release agent.

4. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, is characterized in that, described dispersant comprises one or more mixing in T-2700, NNO, T-2020, KY-555, SHQ-1.

5. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, it is characterized in that, described wetting agent comprises aliphatic alcohol sulfate, alkyl sulfate, alkylol polyoxyethylene groups ether sodium sulfate, alkylphenol-polyethenoxy base ether sodium sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, sodium alkyl sulfonate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, dialkyl succinylsuccinate sulfonate, monoalkyl phenyl polyoxyethylene ether succinic acid sodium sulfonate, fatty alcohol ethylene oxide adduct sulfonate, alkylphenol formaldehyde condensation product ethylene oxide adduct sulfonate, alkane phthalein amido taurate, T-1004, one or more mixing in K12.

6. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, is characterized in that, described binding agent comprises xanthans, carboxymethyl cellulose, soluble starch, bentonite, one or more mixing in polyethylene glycol.

7. the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, is characterized in that, described disintegrant comprises one or more mixing in ammonium sulfate, sodium sulphate, sodium chloride, urea, soluble starch.

8. the preparation method of the uvioresistant agricultural water dispersible granule containing natamycin according to claim 1, it is characterized in that, by formula rate claimed in claim 1, after natamycin sustained release agent, dispersant, wetting agent, binding agent, disintegrant, blue algae powder are uniformly mixed, pulverizing obtains 500～800 order fine powders, in fine powder, add suitable quantity of water, mix rear extruder grain, dry, sieve and obtain water dispersible granules.