CN103636798B - A kind of preparation method of cow-goat milk mixed-type infant formula milk powder - Google Patents

Abstract

The invention discloses a method for preparing cow's and goat's milk mixed formula milk powder for infants and young children. The cow's milk casein is modified, and goat's milk is added according to the ratio of 40:60 according to the ratio of casein and whey protein in human milk. Beta-casein and demineralized whey powder. The protein composition and ratio of the present invention are closer to breast milk, can significantly improve the immunity and resistance of the baby's body, promote the growth of the baby's brain, bones, muscles, fat and overall growth and development, are easier to digest and absorb, and will not cause stomach problems. Discomfort, babies will not suffer from constipation after drinking, it does not contain heterosexual proteins that can cause allergies, and will not cause diarrhea and other dairy product allergy symptoms. It is suitable for babies of any constitution, especially those with weak gastrointestinal tract and poor constitution.

Description

A kind of preparation method of cow-goat milk mixed infant formula milk powder

technical field

The invention relates to a method for preparing infant formula milk powder mixed with cow and goat milk, and belongs to the technical field of dairy product processing.

Background technique

Breast milk is the most ideal natural food for newborns. However, due to the influence of various factors of infants or mothers, about 30%-50% of infants in China cannot be breast-fed at present, so choosing suitable formula milk powder is very important for their growth. Development is very important. There are many types of infant formula milk powders available on the market, most of which use cow's milk as the main raw material, and there are fewer formula milk powders with goat's milk as the main raw material. In fact, from the perspective of infant nutrition, goat milk is rich in various nutrients and is closer to breast milk, especially in terms of protein composition and properties, essential amino acid content, non-protein nitrogen composition, fat properties and fatty acid composition, and oligomerization. The type and content of sugar are superior to cow milk. Milk protein is the most important component in milk. Milk protein mainly includes casein and whey protein. There is no significant difference in the content of casein in goat milk and cow milk. In terms of protein types, cow milk is dominated by αs-CN, while goat milk has the most β-CN content. Human milk does not contain αs-CN, and goat milk contains less αs-CN and a large amount of β-CN. Cow's milk is closer to human milk.

Many properties of human milk, such as strong antibacterial activity, anti-infection ability, hormone-like function and immune function biological activities are closely related to β-casein. Infant formula milk powder does not have some related functions of human milk. Goat milk β-casein content is not only close to that of human milk, but also its structure is similar to breast milk. Calcium in goat milk mainly exists in the form of casein, which is easily absorbed by the body. It is easy for babies to drink, does not contain allergens, and will not cause constipation. It is suitable for babies of any constitution, especially those with weak gastrointestinal tract and poor constitution.

In terms of infant formula milk powder, the past researches simply changed the protein composition of cow milk or goat milk, or artificially added some biologically active substances. This patent focuses on the original infant formula milk powder, inventing a suitable A new type of cow-goat milk mixed infant formula milk powder for 3-month-old infants.

Contents of the invention

The present invention relates to a kind of preparation method of cow and goat milk mixed type infant formula milk powder, and its technical scheme is as follows:

(1) Separating milk casein from fresh milk to obtain milk casein and separation liquid, and then using rennet to hydrolyze and modify milk casein;

(2) Add modified cow's milk casein, goat's β-casein and desalinated whey powder to the cow's milk separation liquid obtained in step (1), and adjust the ratio of casein to whey protein in the formula milk powder to be 40: 60;

(3) Homogenize, sterilize and dry the mixed solution obtained in step (2) to obtain a powdery base powder;

(4) Add ingredients to the powdery base powder obtained in step (3) to prepare infant formula milk powder.

The ingredients in the preparation method are: carbohydrates, dietary fiber, fatty acids, vitamins and minerals.

The preparation method, the ingredients are: energy 2016kJ/100g, carbohydrates 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaene Acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/ 100g, lecithin 182mg/100g, nucleotide 15mg/100g, taurine 27mg/100g, inositol 39mg/100g, L-carnitine 8.6mg/100g, chlorine ≤ 902mg/100g, biotin 14.0μg/100g, bile Alkali 43.1mg/100g, Sodium≤381mg/100g, Zinc 3.41mg/100g, Selenium 12.0μg/100g, Iodine 44μg/100g, Copper 245μg/100g, Manganese 40～44μg/100g, Phosphorus 451mg/100g, Potassium 461mg/100g , magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, and the rest are milk solids and fat.

The rennet described in the preparation method is calf rennet.

The addition amounts of goat milk β-casein and desalted whey powder in step (2) of the preparation method are 20g/100g-30g/100g and 30g/100g-45g/100g respectively.

The preferred addition amount of goat milk β casein is 25g/100g, and the preferred addition amount of desalted whey powder is 37.5g/100g.

In the preparation method, the homogenization pressure in step (3) is 15-20MPa, and the homogenization temperature is 40-50°C.

In the preparation method, the sterilization temperature in step (3) is 82-90°C, and the sterilization time is 20-25s.

Described preparation method, concrete steps are as follows:

(1) Separating milk casein from fresh milk to obtain milk casein and separation liquid, and then using rennet to hydrolyze and modify milk casein;

(2) Adding modified cow milk casein, goat milk β-casein and desalted whey powder to the milk separation liquid obtained in step (1), the amount of the goat milk β-casein added is 20g/100g～ 30g/100g, the added amount of desalted whey powder is 30g/100g～45g/100g, and the ratio of casein to whey protein in formula milk powder is adjusted to 40:60;

(3) Homogenize the mixed liquid obtained in step (2) under high pressure, the homogenization pressure is 15-20MPa, and the homogenization temperature is 40-50°C;

(4) Control the sterilization temperature at 82-90°C, and the sterilization time is 20-25s;

(5) Use a pressure down-flow multi-nozzle drying tower for spray drying to obtain a powdery base powder;

(6) mixing carbohydrates, dietary fiber, fatty acids, vitamins and minerals with the powdery base powder obtained in step (5) to obtain formula milk powder for infants and young children;

(7) Packaging.

Furthermore, the specific steps of the above-mentioned preparation method are as follows:

(1) Milk casein modification: separate milk casein from fresh milk, obtain milk casein and separation liquid, and then use rennet to hydrolyze and modify casein;

(2) Adjusting the protein ratio: adding modified cow's milk casein, goat's milk β-casein and desalted whey powder to the cow's milk separation liquid obtained in step (1), the addition amount of the goat's milk beta-casein is 25g/100g, the amount of desalted whey powder added is 37.5g/100g, and the ratio of casein to whey protein in formula milk powder is adjusted to 40:60;

(3) Homogenization: The mixed solution obtained in step (2) is homogenized under high pressure, the homogenization pressure is 16MPa, and the homogenization temperature is 45°C;

(4) Sterilization: Control the sterilization temperature at 85°C, and the sterilization time is 24s;

(5) Spray drying: use a pressure downstream multi-nozzle drying tower for spray drying to obtain powdery base powder;

(6) Dry blending: mixing carbohydrates, dietary fiber, fatty acids, vitamins, minerals and other nutrients with the powdered base powder obtained in step (5) to obtain formula milk powder for infants and young children;

(7) Packaging: After the formula milk powder is completely exhausted, seal it tightly.

Beneficial effects of the present invention: the protein composition and ratio of the formula milk powder provided by the present invention are closer to breast milk. Spleen index experiment shows that the new infant formula milk powder can significantly improve the immunity and resistance of the infant body. The data of the mouse swimming test showed that the new infant formula has more anti-fatigue ability than ordinary infant formula. Through the verification of bone growth experiments in rats, the data show that the new infant formula milk powder has outstanding performance in promoting bone and overall growth and development. Through hydrolyzed milk solids experiment and goat milk β-casein experiment, the influence of the allergenic heterosexual protein αs-casein in milk was eliminated. The experimental results show that the new infant formula milk powder can effectively prevent diarrhea and other dairy product allergies occurrence of symptoms.

Description of drawings

Fig. 1 Contrast electrophoresis diagram of cow milk casein modification.

Figure 2 Chromatogram of goat milk β-casein purified by DEAE-Sepharose Fast Flow.

Figure 3 SDS-PAGE analysis chart of goat milk β-casein DEAE-Sepharose Fast Flow anion exchange chromatographic elution peak; (M: Marker; 1: Peak Ⅰ; 2: Peak Ⅱ Goat milk β-casein; 3: Peak Ⅲ ; 4: Peak IV).

Figure 4 Effects of different milk powders on bone growth in rats.

Detailed ways

Materials and Reagents

Fresh goat milk is collected from Fuwang Dairy Goat Farm in Suiling County, Heilongjiang Province; fresh cow milk is collected from Harbin Songhuajiang Dairy Farm. Healthy female Kunming first-class mice, 45-60 days old, 18-20g, provided by Harbin Animal Research Institute. Urea, Solarbio Company; Sigma Company; Trypsin, Sigma Company; Sodium Chloride, Tianjin Kemiou Chemical Reagent Development Center; Hydrochloric acid, Tianjin Kemiou Chemical Reagent Development Center; 95% ethanol (v/v), the United States Lai Biological Technology Co., Ltd.; ammonium persulfate (AP), Tianjin Kemiou Chemical Reagent Development Center.

Instruments and Equipment

Tris, Glycine, Sodium Dodecyl Sulfonate (SDS), β-Mercaptoethanol (2-ME), N,N'-Methylenebisacrylamide, Acrylamide, Glycerin, Coomassie Brilliant Blue (CBB) R- 250. Ammonium persulfate (AP), bromophenol blue, hydrochloric acid, ethanol with a volume fraction of 95%, methanol, and glacial acetic acid are of analytical grade; urea, sodium chloride, and DEAE-Sepharose Fast Flow; ultrapure water.

GL-21M high-speed refrigerated centrifuge; DELTA320pH meter; PL2002 electronic balance; small freeze dryer; DYY-10C electrophoresis apparatus; AKTA purifer100 protein purification apparatus; ziptip C18 chromatography column.

Embodiment 1: the modification of cow's milk casein

(1) Extraction and activity determination of rennet enzyme

Calf abomasum→cleaning→fine cutting→extraction (three times)→filtering→dialysis→drying→Renminase rennetase activity determination, please refer to the product manual of Wuxi Enzyme Preparation Factory.

(2) Separation of cow milk casein

Centrifuge the fresh milk to remove fat, adjust the pH of the milk to 4.3 with hydrochloric acid, and collect the precipitate, which is casein.

(3) Human emulsification modification and optimization experiment of casein

Dissolve the casein precipitate collected in the previous step.

Casein solution → rennet enzyme action → enzyme inactivation → centrifugation, the supernatant is the modified casein, detected by SDS-PAGE gel electrophoresis, it is found that compared with the original casein, the position of αs-casein The electrophoresis bands weakened a lot, but the intensity of the electrophoresis bands at the position of β casein did not decrease relatively. This shows that the casein modified by human emulsification has successfully removed a large amount of αs-casein, while retaining most of β-casein.

From the above results, it can be seen that in the modified milk solids, αs-casein, which is abundant in bovine milk but hardly exists in human milk, and which will become the main obstacle for infants to digest and absorb bovine milk, is hydrolyzed, and human αs-casein is retained. Beta-casein, the main protein in milk, is not hydrolyzed.

Embodiment 2: Separation and purification of goat milk β-casein

(1) Fat removal

Select fresh goat milk sample (10mL) and centrifuge at 10400g for 30min at 4°C. The sample is divided into three layers, wherein the milk fat part is in the upper layer, the whey part is in the middle layer, and the milk particle part is in the bottom layer. Avoid the upper lipid layer and absorb the whey part. spare.

(2) Separation of β-casein

Adjust the whey pH to 4.6, reach the isoelectric point of casein, centrifuge at 40000g for 30min at 4°C, collect the precipitate, weigh 100mg of casein precipitate and dissolve in 50mL of equilibrium buffer (20mmol/L Tris, 3.3mol/L Tris urea), 1M NaOH to adjust the pH to 9.5. The samples were stirred intermittently in a water bath at 20°C for 0.5-1h. After fully dissolving, centrifuge at 10,000 g for 30 min at 4°C, filter the supernatant with a 0.45 μm filter membrane, and pass through a DEAE-Sepharose Fast Flow ion exchange column. The adsorbed protein was eluted with a continuous gradient of 0.3mol/L NaCl, and the elution peak at A _280nm was collected. The purity of the eluted peak was determined by SDS-PAGE electrophoresis. According to the properties and literature, peak 2 was determined to be goat milk β-casein, which was dialyzed and then freeze-dried.

From the above results, it can be seen that goat milk β-casein can be separated in vitro, and the α-casein that is easy to cause digestion and absorption disorders and allergies in babies is removed. Babies will not experience constipation after drinking it, and it is suitable for babies of any constitution.

Embodiment 3: Addition of demineralized whey powder

Simulating the ratio of casein to whey protein in human milk, artificially added desalted whey powder, so that the ratio of casein to whey protein in formula milk powder was 40:60.

Embodiment 4: preparation method

Taking the addition amount of goat milk beta casein as 25g/100g and the addition amount of desalted whey powder as 37.5g/100g as an example, the specific steps of the preparation method are as follows:

(1) Separating milk casein from fresh milk to obtain milk casein and separation liquid, and then using rennet to hydrolyze and modify milk casein;

(2) Adding modified cow milk casein, goat milk β-casein and desalted whey powder to the milk separation liquid obtained in step (1), the addition amount of the goat milk β-casein is 25g/100g, The amount of desalted whey powder added is 37.5g/100g, and the ratio of casein to whey protein in formula milk powder is adjusted to 40:60;

(3) Homogenize the mixed liquid obtained in step (2) under high pressure, the homogenization pressure is 16MPa, and the homogenization temperature is 45°C;

(4) Control the sterilization temperature at 85°C, and the sterilization time is 24s;

(5) Use a pressure down-flow multi-nozzle drying tower for spray drying to obtain a powdery base powder;

(6) mixing carbohydrates, dietary fiber, fatty acids, vitamins and minerals with the powdery base powder obtained in step (5) to obtain formula milk powder for infants and young children;

(7) Packaging.

Embodiment 5: a kind of infant formula milk powder

An infant formula milk powder, on the basis of the original infant formula, cow milk casein is modified, goat milk β-casein and cow milk desalted whey powder are added to simulate the content of casein and whey protein in human milk , adjust the ratio of the two to 40:60.

The formula of an infant formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g , Lecithin 182mg/100g, Nucleotide 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0μg/100g, iodine 44μg/100g, copper 245μg/100g, manganese 40～44μg/100g, phosphorus 451mg/100g, potassium 461mg/100g, Magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, goat milk β-casein 20g/100g, desalted whey powder 30g/100g, and the rest are modified milk solids and fat.

Example 6

The formula of an infant formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g , Lecithin 182mg/100g, Nucleotide 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0μg/100g, iodine 44μg/100g, copper 245μg/100g, manganese 40～44μg/100g, phosphorus 451mg/100g, potassium 461mg/100g, Magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, goat milk β-casein 22.5g/100g, desalted whey 33.75g/100g and the rest are modified milk solids and fat.

Example 7

The formula of an infant formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g , Lecithin 182mg/100g, Nucleotide 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0μg/100g, iodine 44μg/100g, copper 245μg/100g, manganese 40～44μg/100g, phosphorus 451mg/100g, potassium 461mg/100g, Magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, goat milk β-casein 25g/100g, desalted whey 37.5g/100g and the rest are modified milk solids and fat.

Example 8

The formula of an infant formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g , Lecithin 182mg/100g, Nucleotide 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0μg/100g, iodine 44μg/100g, copper 245μg/100g, manganese 40～44μg/100g, phosphorus 451mg/100g, potassium 461mg/100g, Magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, goat milk β-casein 27.5g/100g, desalted whey powder 41.25g/100g, and the rest are modified milk solids and Fat.

Example 9

The formula of an infant formula milk powder is: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, Arachidonic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g , Lecithin 182mg/100g, Nucleotide 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g, sodium≤381mg/100g, zinc 3.41mg/100g, selenium 12.0μg/100g, iodine 44μg/100g, copper 245μg/100g, manganese 40～44μg/100g, phosphorus 451mg/100g, potassium 461mg/100g, Magnesium 40.7mg/100g, calcium 561mg/100g, iron 7.42mg/100g, goat milk β-casein 30g/100g, desalted whey powder 45g/100g, and the rest are modified milk solids and fat.

Embodiment 10 Novel formula milk powder improves immunity experiment

Take 45-60-day-old mice (whole mother), divide them into control group, test group 1, test 2, 3 groups, 10 in each group, a total of 30 mice, respectively fed with normal saline, ordinary milk powder, and implement Example 6 New infant formula milk powder (concentration is 1g/ml) 4mL/mouse, once a day, continuous medication for 10 days, then kill the mice in the control group, experimental group 1, experimental group 2 by spinal dislocation, weigh the mice, and take out the spleen weighing. The ratio of spleen weight/mg to body weight/g was used as the spleen index. The effects of different preparations on the spleen index of mice are shown in Table 1.

Table 1 Effects of different milk powders on the spleen index of mice

As shown in Table 1, after data analysis, it was found that the spleen index difference between the control group and the test group 2 was extremely significant (p<0.01); the spleen index between the control group and the test group 1 had a significant difference (p<0.05 ).

From the above experiments, the spleen index of mice can be significantly increased after adding ordinary infant formula milk powder and new formula milk powder in the feed, but the spleen index increases more after adding new formula milk powder, indicating that the new formula milk powder is more effective than ordinary infant formula. Formula milk powder can improve immunity even more.

Embodiment 11 novel formula milk powder anti-fatigue experiment

Swimming test The mice were gavaged continuously for 10 days according to Table 1, and their body weights were weighed 60 minutes after the last gavage. Then tie a 2.0g weight to the base of the tail of the mouse, put the mouse into a swimming pool with a size of 45cm×30cm×20cm and a water temperature of 18.5°C, and start timing. When the mouse’s head was submerged in the water for 10 seconds and could not emerge from the water, it was considered to be physically exhausted. Timed immediately, it was the swimming time of the mouse. The effects of different preparations on the swimming time of the mice are shown in Table 2.

Table 2 Effects of different milk powders on the swimming time of mice

The above experiments show that the new infant formula milk powder has obvious improvement in anti-fatigue compared with ordinary infant formula milk powder.

Example 12 Novel formula milk powder promotes bone development experiment

Select 3-week-old clean-grade pure-line male SD rats and feed them with common feed for 4 weeks, then force-feed 0.8g/d for 10 days. The control group was the experimental group fed with normal saline, the experimental group 1 and the experimental group 2 were the experimental groups fed with common formula milk powder and new infant formula milk powder, respectively. On the 6th day of intragastric administration, the fluorescent matrix-tetracycline was injected intraperitoneally, and the second injection of the fluorescent matrix-tetracycline was performed on the 8th day. On the 10th day, the rats were anesthetized to death, and the tibia was completely stripped out. The distance between the end articular surfaces is the length of the tibia after feeding for 10 days, and the average value of three measurements is taken, and the results are shown in Figure 4. Figure 4 shows that the growth length of the tibia in the control group 0 was 65 μm, the growth length of the tibia in the experimental group 1 was 75 μm, and the growth length of the tibia in the experimental group 2 was 82 μm. The new infant formula has a significant performance in promoting bone development.

Claims (4)

1. a preparation method of cow and goat milk mixed type infant formula milk powder, is characterized in that, the method is as follows: (1) separating bovine milk casein from fresh milk, obtaining bovine milk casein and separating liquid, and then utilizing rennet to hydrolyze and modify bovine milk casein; (2) Add modified cow's milk casein, goat's milk β-casein and desalted whey powder to the cow's milk separation liquid obtained in step (1), and the addition amount of the goat's milk beta casein is 20g/100g～ 30g/100g, the added amount of desalted whey powder is 30g/100g～45g/100g, and the ratio of casein to whey protein in formula milk powder is adjusted to 40:60; (3) Homogenize the mixed solution obtained in step (2) by high pressure, the homogenization pressure is 15-20MPa, and the homogenization temperature is 40-50°C; (4) Control the sterilization temperature at 82-90°C, and the sterilization time is 20-25s; (5) adopting the pressure down-flow type multi-nozzle drying tower to carry out spray drying to obtain powdery base powder; (6) mixing the ingredients with the powdered base powder obtained in step (5) to obtain formula milk powder for infants and young children; The ingredients are: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, peanut Tetraenoic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, Vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g, lecithin 182mg /100g, Nucleotides 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g , Sodium≤381mg/100g, Zinc 3.41mg/100g, Selenium 12.0μg/100g, Iodine 44μg/100g, Copper 245μg/100g, Manganese 40～44μg/100g, Phosphorus 451mg/100g, Potassium 461mg/100g, Magnesium 40.7mg/ 100g, calcium 561mg/100g, iron 7.42mg/100g, the rest is milk solids and fat; (7) Packaging. 2. the described preparation method of claim 1 is characterized in that, the rennet described in step (1) is calf rennet. 3. The preparation method according to claim 1, characterized in that, the addition amount of goat milk β-casein is 25g/100g, and the addition amount of desalted whey powder is 37.5g/100g. 4. the described preparation method of claim 1 is characterized in that, concrete steps are as follows: (1) Cow's milk casein modification: separate cow's milk casein from fresh milk, obtain cow's milk casein and separation liquid, and then use rennet to hydrolyze and modify the casein; (2) Adjust the protein ratio: add modified cow's milk casein, goat's milk β-casein and desalted whey powder to the cow's milk separation liquid obtained in step (1), and the addition amount of the goat's milk beta casein is 25g/100g, the amount of desalted whey powder added is 37.5g/100g, and the ratio of casein to whey protein in formula milk powder is adjusted to 40:60; (3) Homogenization: the mixed solution obtained in step (2) is homogenized under high pressure, the homogenization pressure is 16MPa, and the homogenization temperature is 45°C; (4) Sterilization: control the sterilization temperature at 85°C, and the sterilization time is 24s; (5) Spray drying: adopt the pressure down-flow type multi-nozzle drying tower to carry out spray drying to obtain powdery base powder; (6) Dry blending: mixing the ingredients with the powdery base powder obtained in step (5) to obtain formula milk powder for infants and young children; The ingredients are: energy 2016kJ/100g, carbohydrate 26.1g/100g, dietary fiber 199mg/100g, linoleic acid 1925mg/100g, α-linolenic acid 304mg/100g, docosahexaenoic acid 6867μg/100g, peanut Tetraenoic acid 13.5mg/100g, Vitamin A 469μg/100g, Vitamin D 7.1μg/100g, Vitamin E 4.82mg/100g, Vitamin K ₁ 25.2μg/100g, Vitamin B ₁ 355μg/100g, Vitamin B ₂ 372μg/100g, Vitamin B ₆ ≥240μg/100g, vitamin B ₁₂ ≥1.25μg/100g, vitamin C 45.8mg/100g, niacin 2785μg/100g, folic acid 30μg/100g, pantothenic acid 2050μg/100g, oligosaccharides 411mg/100g, lecithin 182mg /100g, Nucleotides 15mg/100g, Taurine 27mg/100g, Inositol 39mg/100g, L-Carnitine 8.6mg/100g, Chlorine≤902mg/100g, Biotin 14.0μg/100g, Choline 43.1mg/100g , Sodium≤381mg/100g, Zinc 3.41mg/100g, Selenium 12.0μg/100g, Iodine 44μg/100g, Copper 245μg/100g, Manganese 40～44μg/100g, Phosphorus 451mg/100g, Potassium 461mg/100g, Magnesium 40.7mg/ 100g, calcium 561mg/100g, iron 7.42mg/100g, the rest is milk solids and fat; (7) Packaging: After the formula milk powder is completely exhausted, seal it tightly.