CN102706874A - Reagent and method for detecting copper ion content in water environment - Google Patents
Reagent and method for detecting copper ion content in water environment Download PDFInfo
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- CN102706874A CN102706874A CN2012102264274A CN201210226427A CN102706874A CN 102706874 A CN102706874 A CN 102706874A CN 2012102264274 A CN2012102264274 A CN 2012102264274A CN 201210226427 A CN201210226427 A CN 201210226427A CN 102706874 A CN102706874 A CN 102706874A
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- copper
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- water
- copper ion
- organic phase
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 229910001431 copper ion Inorganic materials 0.000 title claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title abstract description 7
- 239000012074 organic phase Substances 0.000 claims abstract description 57
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000010949 copper Substances 0.000 claims abstract description 25
- 229910052802 copper Inorganic materials 0.000 claims abstract description 25
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims abstract description 18
- ORIHZIZPTZTNCU-YVMONPNESA-N salicylaldoxime Chemical group O\N=C/C1=CC=CC=C1O ORIHZIZPTZTNCU-YVMONPNESA-N 0.000 claims abstract description 17
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims abstract description 14
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims abstract description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims abstract description 6
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000003556 assay Methods 0.000 claims description 20
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 claims description 18
- 238000004737 colorimetric analysis Methods 0.000 claims description 4
- 241001597008 Nomeidae Species 0.000 claims description 2
- 239000008096 xylene Substances 0.000 claims description 2
- 230000000536 complexating effect Effects 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 abstract 2
- 229960001701 chloroform Drugs 0.000 abstract 1
- 239000003086 colorant Substances 0.000 abstract 1
- 230000006866 deterioration Effects 0.000 abstract 1
- 230000009514 concussion Effects 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 239000012467 final product Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000009088 enzymatic function Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a reagent and method for detecting copper ion content in a water environment. The reagent is solution in which a copper color-developing agent is dissolved in organic phases; the copper color-developing agent is salicylaldoxime and/or salicylaldoxime derivatives; and the organic phase is one or more of benzene, methylbenzene, dimethylbenzene, styrene, ethyl acetate, carbon tetrachloride, trichloromethane and dichloromethane. The reagent is formed by dissolving the copper color-developing agent in the organic phases; and when the organic phases are fully mixed with water solution, the copper color-developing agent and copper ions are subjected to complexing to enter the organic phases, and the color of the organic phases are changed along with the concentration of the copper ions, so that the concentration of the copper ions can be detected according to the colors of the organic phases. The reagent comprises less components, is low in cost and good in stability, and can be stored for a long time without deterioration; and moreover, as all the organic phases of the reagent and the water are immiscible, waste liquid after detection can be separated and recovered, and can be reused after being processed, so that secondary pollution is avoided.
Description
Technical field
The present invention relates to a kind of analytical reagent and method, the mensuration reagent and the assay method thereof of content of copper ion in especially a kind of water environment.
Background technology
Copper is the very wide trace element of a kind of distribution, and the average abundance of copper is 55ppm in the earth's crust.Copper is the life trace elements necessary, participates in the enzymatic function, also is the ingredient of cupreins such as blood of human body, liver and brain tissue, and the requirement of being grown up every day is estimated as 20mg, but excessive copper all is harmful to people and animals and plants.When copper content reaches 0.01mg/L in the water, the self-purification of water there is the obvious suppression effect, surpasses 3mg/L, can produce peculiar smell, surpass 15mg/L, just can't drink.The copper ion of excessive concentrations can cause the water body bacterium, and the death of mushroom also can cause the damage of human liver and kidney and the wadding unrest of functions of intestines and stomach.Therefore, the inspection of copper ion aspect environmental protection and the health, had very important meaning.
At present, adopt colourimetry to detect the copper in the water quality, required reagent type is more, operates comparatively loaded down with trivial detailsly, and sensing range is narrower, thereby the waste liquid after the detection can't be recycled and is prone to cause secondary pollution.
Summary of the invention
The technical matters that the present invention will solve provides the mensuration reagent that a kind of composition is simple, detect content of copper ion in the callable water environment of back waste liquid.The present invention also provides the assay method of content of copper ion in a kind of water environment that adopts said determination reagent.
For solving the problems of the technologies described above, the technical scheme that the present invention taked is: it is for being dissolved with the solution of copper developer in organic phase; Described copper developer is salicylaldoxime and/or salicylaldoxime derivant; Described organic phase is one or more in benzene,toluene,xylene, styrene, ethyl acetate, phenixin, methenyl choloride and the methylene chloride.
Preferred solution concentration is 0.5g/L～10g/L.
Preferred copper developer is 3-bromo-5-tert-butyl group salicylaldoxime and/or 5-tert-butyl group salicylaldoxime.
The inventive method adopts above-mentioned mensuration reagent, and the mensuration process is: in copper ions water, add and measure reagent, fully mix, judge the concentration of copper ion according to the color employing colourimetry of organic phase.
Preferably, the volume ratio of said mensuration reagent and copper ions water is 1:1～1:3; Best, the volume ratio of said mensuration reagent and copper ions water is 1:1.
Preferably, said copper ions water fully mixes back vibration 3～5 minutes with mensuration reagent.
Preferably, the pH value of said copper ions water is 2～6.
Adopt the beneficial effect that technique scheme produced to be: the present invention is by dissolving forms with the copper developer in the organic phase; When organic phase is fully mixed with the WS; The copper developer will get into organic phase with the copper ion complexing; The color of organic phase changes, and color is along with copper ion concentration changes, thereby according to the color detection copper ion concentration of organic phase.
Reagent composition of the present invention is few, and cost is low, and the stable reagent that is mixed with is good, but long-term storage is never degenerated; Organic phase and water that the present invention is all do not dissolve each other, and the waste liquid after therefore detecting can Separation and Recovery, and treated laggard can the repeated use can not caused secondary pollution.
The inventive method adopts colourimetry to detect, and it is few to measure the reagent demand, has simple to operately, quick, detects the low characteristics of cost.
Embodiment
Below in conjunction with specific embodiment the present invention is done further detailed explanation.
Embodiment 1: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: with 3-bromo-5 tert-butyl group salicylaldoximes is developer, is organic phase with benzene.Take by weighing 1g 3-bromo-5 tert-butyl group salicylaldoximes, be dissolved in the 100ml organic phase, and be transferred in the volumetric flask of 1L, add organic phase again and be settled to 1L, can obtain measuring reagent.
Assay method: get the pH value and be 2 copper ions water; Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 6ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 3min, observe the color of organic phase and can judge the copper ion concentration in this copper ions water environment fast under 25 ℃.
Embodiment 2: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: with 3-bromo-5 tert-butyl group salicylaldoximes is developer, is organic phase with benzene.Take by weighing 0.5g 3-bromo-5 tert-butyl group salicylaldoximes, be dissolved in the 100ml organic phase, and be transferred in the volumetric flask of 1L, add organic phase again and be settled to 1L, can obtain measuring reagent.
Assay method: get the pH value and be 4 copper ions water; Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 10ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 5min, observe the color of organic phase and can judge the copper ion concentration in this copper ions water environment fast under 25 ℃.
Embodiment 3: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: with 3-bromo-5 tert-butyl group salicylaldoximes is developer, is organic phase with benzene.Take by weighing 10g 3-bromo-5 tert-butyl group salicylaldoximes, be dissolved in the 100ml organic phase, and be transferred in the volumetric flask of 1L, add organic phase again and be settled to 1L, can obtain measuring reagent.
Assay method: get the pH value and be 6 copper ions water; Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 12ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 10min, the color of observing organic phase gets final product the copper ion concentration in quick this copper ions water environment under 25 ℃.
Embodiment 4: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: with 5-tert-butyl group salicylaldoxime is developer, is organic phase with the chloroform.Take by weighing 10g 3-bromo-5-tert-butyl group salicylaldoxime, be dissolved in the 100ml organic phase, and be transferred in the volumetric flask of 1L, add organic phase again and be settled to 1L, can obtain measuring reagent.
Assay method: get the pH value and be 6 copper ions water; Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 4ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 10min, the color of observing organic phase gets final product the copper ion concentration in quick this copper ions water environment under 25 ℃.
Embodiment 5: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: the potpourri with 3-bromo-5-tert-butyl group salicylaldoxime and 5 tert-butyl group salicylaldoximes is a developer, is organic phase with toluene.Take by weighing the 3-bromo-5-tert-butyl group salicylaldoxime of 5g, the tert-butyl group salicylaldoxime of 5g is dissolved in the 100ml organic phase, and is transferred in the volumetric flask of 1L, adds organic phase again and is settled to 1L, can obtain measuring reagent.
Assay method: get the pH value and be 6 copper ions water; Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 12ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 10min, the color of observing organic phase gets final product the copper ion concentration in quick this copper ions water environment under 25 ℃.
Embodiment 6: the mensuration reagent and the assay method of content of copper ion is described below in this water environment.
Measure the configuration of reagent: with 3-bromo-5-tert-butyl group salicylaldoxime is developer, is organic phase with toluene.Take by weighing 10g 3-bromo-5-tert-butyl group salicylaldoxime, be dissolved in the 100ml organic phase, and be transferred in the volumetric flask of 1L, add organic phase again and be settled to 1L, can obtain measuring reagent.
Assay method: getting the pH value and be 3 copper ions concentration is the industrial waste water of 60.1mg/L (ICP detection); Get 4ml and measure reagent and the fully mixing in the 25ml color comparison tube of 12ml copper ions water; After copper developer and copper ion complexing get into organic phase; The color of organic phase is along with copper ion concentration changes; Behind the concussion 10min, observe the color of organic phase under 25 ℃, the color of organic phase and 60mg/L copper mark liquid phase are together.
Claims (8)
1. the mensuration reagent of content of copper ion in the water environment, it is characterized in that: it is for being dissolved with the solution of copper developer in organic phase; Described copper developer is salicylaldoxime and/or salicylaldoxime derivant; Described organic phase is one or more in benzene,toluene,xylene, styrene, ethyl acetate, phenixin, methenyl choloride and the methylene chloride.
2. the mensuration reagent of content of copper ion in the water environment according to claim 1 is characterized in that: the concentration of said solution is 0.5g/L～10g/L.
3. the mensuration reagent of content of copper ion in the water environment according to claim 1 and 2 is characterized in that: described copper developer is 3-bromo-5-tert-butyl group salicylaldoxime and/or 5-tert-butyl group salicylaldoxime.
4. the assay method of content of copper ion in the water environment; It adopts claims 1,2 or 3 described mensuration reagent; It is characterized in that: in copper ions water, add and measure reagent, fully mix, judge the concentration of copper ion according to the color employing colourimetry of organic phase.
5. the assay method of content of copper ion in the water environment according to claim 4 is characterized in that: the volume ratio of said mensuration reagent and copper ions water is 1:1～1:3.
6. the assay method of content of copper ion in the water environment according to claim 5 is characterized in that: the volume ratio of said mensuration reagent and copper ions water is 1:1.
7. according to the assay method of content of copper ion in claim 4, the 5 or 6 described water environments, it is characterized in that: said copper ions water fully mixes back vibration 3～5 minutes with mensuration reagent.
8. according to the assay method of content of copper ion in claim 4, the 5 or 6 described water environments, it is characterized in that: the pH value of said copper ions water is 2～6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102264274A CN102706874A (en) | 2012-07-03 | 2012-07-03 | Reagent and method for detecting copper ion content in water environment |
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| CN2012102264274A CN102706874A (en) | 2012-07-03 | 2012-07-03 | Reagent and method for detecting copper ion content in water environment |
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| CN102706874A true CN102706874A (en) | 2012-10-03 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103233286A (en) * | 2013-04-19 | 2013-08-07 | 中国水产科学研究院东海水产研究所 | Production method of nano-fiber for determining copper ion content in water body |
| CN106198522A (en) * | 2016-07-05 | 2016-12-07 | 河南城建学院 | A kind of fast detection kit for copper ions and detection method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1033077A (en) * | 1987-07-28 | 1989-05-24 | Po化学研究院 | From chloride soln, reclaim the method for metal |
| US6546939B1 (en) * | 1990-11-05 | 2003-04-15 | Ekc Technology, Inc. | Post clean treatment |
| CN101386912A (en) * | 2008-10-24 | 2009-03-18 | 南京市华凯化工有限公司 | Copper extractive agent |
| JP2011144437A (en) * | 2010-01-18 | 2011-07-28 | Sumitomo Metal Mining Co Ltd | Method for separating and recovering copper from copper-containing iron sulfide |
| CN102608048A (en) * | 2012-04-13 | 2012-07-25 | 中国科学院唐山高新技术研究与转化中心 | Method for detecting concentration of copper ions in water environment through spectrophotometry |
-
2012
- 2012-07-03 CN CN2012102264274A patent/CN102706874A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1033077A (en) * | 1987-07-28 | 1989-05-24 | Po化学研究院 | From chloride soln, reclaim the method for metal |
| US6546939B1 (en) * | 1990-11-05 | 2003-04-15 | Ekc Technology, Inc. | Post clean treatment |
| CN101386912A (en) * | 2008-10-24 | 2009-03-18 | 南京市华凯化工有限公司 | Copper extractive agent |
| JP2011144437A (en) * | 2010-01-18 | 2011-07-28 | Sumitomo Metal Mining Co Ltd | Method for separating and recovering copper from copper-containing iron sulfide |
| CN102608048A (en) * | 2012-04-13 | 2012-07-25 | 中国科学院唐山高新技术研究与转化中心 | Method for detecting concentration of copper ions in water environment through spectrophotometry |
Non-Patent Citations (3)
| Title |
|---|
| C.K. BHASKARE, S.G. KAWATKAR: "Extractive photometric simultaneous determination of iron(II) and copper(II) with syn-phenyl-α-pyridyl ketoxime and analysis of ferrites", 《TALNNTO》 * |
| 吕文东: "调节剂对烷基水杨醛肟萃取铜铁的影响", 《广东有色金属学报》 * |
| 张艳: "间接原子吸收法测定水杨醛", 《山东大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103233286A (en) * | 2013-04-19 | 2013-08-07 | 中国水产科学研究院东海水产研究所 | Production method of nano-fiber for determining copper ion content in water body |
| CN106198522A (en) * | 2016-07-05 | 2016-12-07 | 河南城建学院 | A kind of fast detection kit for copper ions and detection method thereof |
| CN106198522B (en) * | 2016-07-05 | 2019-02-22 | 河南城建学院 | A kind of copper ion rapid detection kit and detection method thereof |
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Application publication date: 20121003 |