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CN102671235A - High-orientation nanofiber nerve conduit and preparation method thereof - Google Patents

High-orientation nanofiber nerve conduit and preparation method thereof Download PDF

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CN102671235A
CN102671235A CN2012101535940A CN201210153594A CN102671235A CN 102671235 A CN102671235 A CN 102671235A CN 2012101535940 A CN2012101535940 A CN 2012101535940A CN 201210153594 A CN201210153594 A CN 201210153594A CN 102671235 A CN102671235 A CN 102671235A
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nerve guide
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highly oriented
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oriented nanofiber
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冯淑芹
沈新元
冯虎
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Donghua University
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Abstract

The invention relates to a high-orientation nanofiber nerve conduit and a preparation method thereof. The nerve conduit comprises the following components in parts by weight: 2-40 parts of polymer and 0.01-3 parts of carbon nano tube. The length of the nerve conduit is 1mm-10m, the inner diameter is 0.01mm-10cm, and the wall thickness is 0.01mm-5mm. The preparation method for the high-orientation nanofiber nerve conduit comprises the following steps of: 1-, firstly, ultrasonically dispersing the carbon nano tube in an organic solvent; then, adding the polymer; and stirring to prepare a transparent and even spinning solution; 2-, performing electrostatic spinning by adopting the spinning solution; receiving a fiber support by a winding receiving device; taking down the fiber support; and finally, drying to prepare the nerve conduit. The high-orientation nanofiber nerve conduit has the advantages that the biocompatibility of the polymer of the base material is good, and hemolysis or blood coagulation cannot be caused. Moreover, the high-orientation nanofiber nerve conduit has required physical and mechanical performance and processability, is capable of enduring disinfection and is wide in application. The preparation method has the advantages that the operation is simple, and the economic cost is reduced.

Description

A kind of high oriented nanofibers nerve trachea and preparation method thereof
Technical field
The invention belongs to nerve trachea and preparation field thereof, particularly a kind of high oriented nanofibers nerve trachea and preparation method thereof.
Background technology
Nervous system is standard and the complication system of coordinating body movement.It has two main systems: the central nervous system peripheral nervous system of unifying.The central nervous system comprises brain and spinal cord, and peripheral nervous system has related to all nerves from the spinal cord to extremity.Neuron does not possess regeneration capacity in the central nervous system.Therefore, recovering its function is neurologic significant challenge.Recently, the timbering material of utilization organizational project preparation is to replace the important selection that traditional heteroplastic transplantation is repaired.For example; People such as Yang (Yang F.Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering [J] .Biomaterials, 2004) utilize the method for liquid liquid phase-splitting to prepare high porosity and porous fibre polylactic acid bracket material system is used in nervous tissue's engineering.These fibrous frameworks can be supported cell differentiation of nerve cord and enation.Electrostatic spinning be a kind of can producd fibers diameter little simple, effective method to 5nm, can the analog cell epimatrix by the support of nanofiber preparation, for the growth and the breeding of cell provides the environment that is more suitable for.Synthctic fat adoption ester and copolyesters macromolecular material have been made into porous timbering material, to improve nervous tissue's regeneration.
CNT is introduced in the polymer nanofiber, both can be strengthened nanofiber, also can give nanofiber excellent physics and chemical property.CNT has high intensity and great toughness, and high draw ratio (generally greater than 1000), and its structure is unique, physics, chemical characteristic excellence, and the research of carbon nano tube/polymer nano fiber causes people's extensive concern.In recent years; The exploratory development that with the biological applications is purpose CNT/biomolecule based composites increases rapidly; Through integrating the performance of bioactive molecule (enzyme, protein, polysaccharide etc.) and CNT, become a new research focus gradually.
Summary of the invention
Technical problem to be solved by this invention provides a kind of high oriented nanofibers nerve trachea and preparation method thereof; This nerve trachea good biocompatibility, not carcinogenic, content of beary metal is low, pH value is little; Do not cause performances such as haemolysis and blood coagulation; And having required physics, mechanical property and processing characteristics, this method is simple, economical, is fit to suitability for industrialized production.
A kind of high oriented nanofibers nerve trachea of the present invention, this nerve trachea comprises by weight: 2 ~ 40 parts in polymer, 0.01 ~ 3 part of CNT; Described nerve trachea length is 1mm～10m, internal diameter 0.01mm～10cm, wall thickness 0.01mm～5mm.
Described polymer is biodegradable polycaprolactone (PCL), polylactic acid, gather in Acetic acid, hydroxy-, bimol. cyclic ester, the poly (glycolide-co-lactide) block copolymer (PLGA) one or more.
The average diameter of nanofiber is 200nm～700nm in the described high oriented nanofibers nerve trachea, the fibre orientation degree>80%, voidage is 75%～98%.
The method for preparing of a kind of high oriented nanofibers nerve trachea of the present invention comprises the steps:
(1) earlier CNT is dispersed in the organic solvent, behind the ultra-sonic dispersion, adds polymer again, stir and make the transparent and uniform spinning solution; Wherein according to the weight meter, 2 ~ 40 parts in polymer, 97.99 ~ 57 parts of organic solvents, 0.01 ~ 3 part of CNT;
(2) adopt above-mentioned spinning solution to carry out electrostatic spinning, final drying is processed nerve trachea.
CNT described in the step (1) is for the CNT that do not carry out any processing, through the CNT after physical method such as grinding or ultrasonic and handling, a kind of through in the oxide/carbon nanometer tube after the chemical treatment, the CNT after biological treatments such as chitosan or enzyme.
Organic solvent described in the step (1) is N; N dimethyl formamide (DMF), N; N dimethyl acetylamide (DMAc), ethanol, dichloromethane, chloroform, 1, the mixed solvent of one or more in 2-dichloroethanes, oxolane, trichloroethylene, Hexalin, dimethyl sulfoxine, hexafluoroisopropanol, the trifluoroethanol.
The concrete technology of the electrostatic spinning described in the step (2) is: the fltting speed of spinning solution is 1 μ l/h～10ml/h, 0～40 ℃ of ambient temperature, and voltage 5kv～20kv, spout aperture 1 μ m～2mm, the coiling receiving system receives fibrous framework.
The aperture of winch spool is 1mm～50cm in the described coiling receiving system, and rotary speed is 200-10000rpm.
The aperture of winch spool is 1mm～50cm in the coiling receiving system described in the step (2), and rotary speed is 200-10000rpm.
Process nerve trachea for dried fibrous framework is wound into conduit on the conduit plug described in the step (2), described diameter of mandrel is 0.01mm～10cm.
The objective of the invention is to propose a kind of orderly superfine fibre that contains CNT with the preparation of static spinning process; Thereby preparation has the nerve trachea of the cell growth of can exciting nerve; This conduit is beneficial to cell and grows along duct direction, has the effect of the peripheral nerve of reparation.
Can dried fibrous framework be wound into conduit on the conduit plug of the different-diameter of correspondence according to the requirement of different conduit diameters among the present invention; Described diameter of mandrel is 0.01mm～10cm.
CNT among the present invention in the high orientation superfine fibre nerve trachea has cell guiding growth and the ability of conducting; And the fiber of arranging along the nerve growth direction can provide the neurocyte guide channel; The guiding axon growth, thus the neural reparation that is developed as of this high orientation superfine fibre nerve trachea provides a new thinking.
The average diameter of fiber is 200nm～700nm in the high oriented nanofibers nerve trachea of the present invention; The fibre orientation degree>80%, voidage is 75%～98%.Fibrous framework in this conduit extensively not can be used for conduit, can be used for also that cardiac branch, hernia are damaged, the patching material at bone transplantation substitute material, brain lobe, cerebrovascular and carotid artery place, can be used for human body face-liftings such as lip repairing, bridge of the nose finishing in addition.
Beneficial effect:
1, described catheter-based material/polymer have nonhazardous, nonirritant, chemical stability good, degrade, do not cause inflammation, do not have irritated reaction, good biocompatibility, not carcinogenic, content of beary metal is low, pH value is little, does not cause performances such as haemolysis and blood coagulation; And have required physics, mechanical property and processing characteristics, and can stand to disinfect;
2,, have that operational approach is simple, Financial cost reduces through the catheter holder of electrostatic spinning process preparation; But also have the fento effect, and electric property, and mechanical property such as very large specific surface area, flexible and superpower mechanical behavior (like hardness and tensile strength), these excellent characteristics make nanofiber be more suitable for medical and health, fields such as organizational project;
3, will introduce CNT in the high oriented nanofibers nerve trachea of the present invention, strengthen the mechanical property of fibrous framework, also give the excellent physical and the chemical property of superfine fibre support;
4, high oriented nanofibers nerve trachea of the present invention not only structurally can effectively imitate extracellular matrix, and the orientations of its fiber is beneficial to neural along fiber orientation directions growth extension.
Description of drawings
Fig. 1 is the surface scan figure of the nerve trachea prepared of the present invention;
Fig. 2 is the nerve trachea projection Electronic Speculum figure that the embodiment of the invention 2 is prepared.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) preparation of fibrous framework
Figure BDA00001643831700031
Figure BDA00001643831700041
Earlier with CNT be dispersed in carry out ultra-sonic dispersion in the organic solvent after, again with polymer dissolution in scattered solution, stir with magnetic stirring apparatus and to make the transparent and uniform electrostatic spinning solution; According to the weight meter, 20 parts of PCL, 77 parts of solvents, 3 parts of CNTs.
Prepared static spinning stock solution is carried out static to be spun.Spinning parameter is controlled at voltage 15kv, spout aperture 0.4mm, solution flow rate 0.5ml/h; 0 ℃ of ambient temperature, winch spool (diameter is 5cm) and spout spacing 15cm, winding speed is controlled at 10000rpm; The fltting speed of spinning solution is 0.05ml/h, and spinning 12h finishes reaction.Fibre diameter is 200nm in the conduit; Fibre orientation degree 90%, porosity are 85%.
(2) nerve trachea preparation
After static spins end, take off fibrous framework, be immersed in 48h in the organic solvent ethanol, carry out vacuum drying again from winch spool.At diameter is that the conduit plug upper edge of 0.5mm fiber orientation directions and is wound into conduit.
Embodiment 2
(1) preparation of fibrous framework
Figure BDA00001643831700042
The 100g CNT is joined (dense HSO in the mixed acid solution of 100ml 4With dense HNO 3Mixing), ultra-sonic dispersion 2 ~ 3 hours (h) carries out oxidation in the room temperature magnetic agitation then.Through Merlon filter paper vacuum filtration mixture, be 7 by deionized water wash to pH value again.CNTs after the processing is subsequent use behind the dry 24h with vacuum drying oven at 80 ℃.
After the CNT that oxidation processes is crossed is dispersed in and carries out ultra-sonic dispersion in the organic solvent, again with polymer dissolution in scattered solution, stir with magnetic stirring apparatus and to make the transparent and uniform electrostatic spinning solution; According to the weight meter, PLLA2 part, 97.8 parts of solvents, 0.2 part of CNT.
Prepared static spinning stock solution is carried out static to be spun.Spinning parameter is controlled at voltage 20kv, spout aperture 0.7mm, solution flow rate 2ml/h; 25 ℃ of ambient temperatures, winch spool (diameter is 50cm) and spout spacing 5cm, winding speed is controlled at 2000rpm; The fltting speed of spinning solution is 0.5ml/h, finishes reaction behind the spinning 1h.Fibre diameter is 250nm in the conduit; Fibre orientation degree 80%, porosity are 75%.
(2) nerve trachea preparation
After static spins end, take off fibrous framework, be immersed in 48h in the organic solvent ethanol, carry out vacuum drying again from winch spool.At diameter is that the conduit plug upper edge of 5mm fiber orientation directions and is wound into conduit.
The performance of the oriented nanofibers of high oriented nanofibers nerve trachea of the present invention embodies in the accompanying drawings.
Embodiment 3
(1) preparation of fibrous framework
Figure BDA00001643831700051
After the CNT that treatment with chitosan is crossed is dispersed in and carries out ultra-sonic dispersion in the organic solvent, again with polymer dissolution in scattered solution, stir with magnetic stirring apparatus and to make the transparent and uniform electrostatic spinning solution; According to the weight meter, 5 parts of PLGA, 94 parts of solvents, 1 part of CNT.
Prepared static spinning stock solution is carried out static to be spun.Spinning parameter is controlled at voltage 5kv, spout aperture 1mm, and solution flow rate 0.5ml/h, 25 ℃ of ambient temperatures, winch spool (diameter is 5cm) and spout spacing 5cm, winding speed is controlled at 200rpm, and the fltting speed of spinning solution is 1ml/h, spinning 4h.Fibre diameter is 550nm in the conduit; Fibre orientation degree 85%, porosity are 88%.
(2) nerve trachea preparation
After static spins end, take off fibrous framework, be immersed in 48h in the organic solvent ethanol, carry out vacuum drying again from winch spool.At diameter is that the conduit plug upper edge of 10mm fiber orientation directions and is wound into conduit.
Embodiment 4
(1) preparation of fibrous framework
Figure BDA00001643831700052
After the CNT that treatment with chitosan is crossed is dispersed in and carries out ultra-sonic dispersion in the organic solvent, again with polymer dissolution in scattered solution, stir with magnetic stirring apparatus and to make the transparent and uniform electrostatic spinning solution; According to the weight meter, 10 parts of PGA, 90 parts of solvents, 0.5 part of CNT.
Prepared static spinning stock solution is carried out static to be spun.Spinning parameter is controlled at voltage 20kv, spout aperture 1mm, and solution flow rate 1ml/h, 25 ℃ of ambient temperatures, winch spool (diameter is 0.5cm) and spout spacing 20cm, winding speed is controlled at 4000rpm, and the fltting speed of spinning solution is 2ml/h, spinning 2h.Fibre diameter is 600nm in the conduit; Fibre orientation degree 85%, porosity are 98%.
(2) nerve trachea preparation
After static spins end, take off catheter holder, be immersed in 48h in the organic solvent ethanol, carry out vacuum drying again from winch spool.Being cut into diameter is 0.5cm, and length is 0.5m, and thickness is the nerve trachea of 0.2mm.

Claims (10)

1.一种高取向纳米纤维神经导管，该神经导管按重量份数包括：聚合物2~40份，碳纳米管0.01~3份；所述的神经导管长度为1mm～10m，内径0.01mm～10cm，壁厚0.01mm～5mm。1. A highly oriented nanofiber nerve guide, which comprises by weight: 2 to 40 parts of polymer, 0.01 to 3 parts of carbon nanotube; the length of the nerve guide is 1 mm to 10 m, and the inner diameter is 0.01 mm to 10cm, wall thickness 0.01mm ~ 5mm. 2.根据权利要求1所述的一种高取向纳米纤维神经导管，其特征在于：所述的聚合物为生物可降解聚己内酯、聚乳酸、聚乙交酯、聚乙交酯丙交酯嵌段共聚物中的一种或几种。2. A kind of highly oriented nanofiber nerve guide according to claim 1, characterized in that: said polymer is biodegradable polycaprolactone, polylactic acid, polyglycolide, polyglycolide lactide One or more of ester block copolymers. 3.根据权利要求1所述的一种高取向纳米纤维神经导管，其特征在于：所述的高取向纳米纤维神经导管中纳米纤维的平均直径为200nm～700nm，纤维取向度>80%，空隙率为75%～98%。3. A highly oriented nanofiber nerve guide according to claim 1, characterized in that: the average diameter of the nanofibers in the highly oriented nanofiber nerve guide is 200 nm to 700 nm, the degree of fiber orientation is >80%, and the gap The rate is 75% to 98%. 4.一种高取向纳米纤维神经导管的制备方法，包括如下步骤：4. A preparation method for a highly oriented nanofiber nerve guide, comprising the steps of: （1）先将碳纳米管分散在有机溶剂中，超声分散后，再加入聚合物，搅拌制得透明均匀的纺丝溶液；其中按照重量计，聚合物2~40份，有机溶剂97.99~57份，碳纳米管0.01~3份；(1) First disperse the carbon nanotubes in an organic solvent, ultrasonically disperse, then add the polymer, and stir to obtain a transparent and uniform spinning solution; in which, by weight, 2~40 parts of the polymer, 97.99~57 parts of the organic solvent parts, carbon nanotubes 0.01~3 parts; （2）采用上述纺丝溶液进行静电纺丝，最后干燥，制成神经导管。(2) The above spinning solution is used for electrospinning, and finally dried to make a nerve guide. 5.根据权利要求4所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：步骤（1）中所述的碳纳米管为未进行任何处理的碳纳米管、经过物理方法处理后的碳纳米管、经过化学处理后的氧化碳纳米管、经过生物处理后的碳纳米管中的一种。5. A method for preparing a highly oriented nanofiber nerve guide according to claim 4, characterized in that: the carbon nanotubes described in step (1) are carbon nanotubes that have not undergone any treatment and have been treated by physical methods One of carbon nanotubes after carbon nanotubes, oxidized carbon nanotubes after chemical treatment, and carbon nanotubes after biological treatment. 6.根据权利要求5所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：所述的物理方法为研磨或超声，所述的生物处理为经壳聚糖或酶处理。6. A method for preparing a highly oriented nanofiber nerve guide according to claim 5, characterized in that: the physical method is grinding or ultrasonication, and the biological treatment is chitosan or enzyme treatment. 7.根据权利要求4所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：步骤（1）中所述的有机溶剂为N，N二甲基甲酰胺、N，N二甲基乙酰胺、乙醇、二氯甲烷、三氯甲烷、1，2-二氯乙烷、四氢呋喃、三氯乙烯、环己醇、二甲亚砜、六氟异丙醇、三氟乙醇中的一种或几种的混合溶剂。7. The preparation method of a highly oriented nanofiber nerve guide according to claim 4, characterized in that: the organic solvent described in step (1) is N,N dimethylformamide, N,N dimethylformamide Acetamide, ethanol, dichloromethane, chloroform, 1,2-dichloroethane, tetrahydrofuran, trichloroethylene, cyclohexanol, dimethyl sulfoxide, hexafluoroisopropanol, trifluoroethanol One or more mixed solvents. 8.根据权利要求4所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：步骤（2）中所述的静电纺丝的具体工艺为：纺丝溶液的推进速度为1μl/h～10ml/h，环境温度0～40℃，电压5kv～20kv，喷口孔径1μm～2mm，卷绕接收装置接收纤维支架。8. The preparation method of a highly oriented nanofiber nerve guide according to claim 4, characterized in that: the specific process of electrospinning described in step (2) is: the propulsion speed of the spinning solution is 1 μl/ h～10ml/h, ambient temperature 0～40℃, voltage 5kv～20kv, nozzle aperture 1μm～2mm, winding receiving device to receive fiber support. 9.根据权利要求8所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：所述的卷绕接收装置中卷绕筒的孔径为1mm～50cm，旋转速度为200-10000rpm。9. The method for preparing a highly oriented nanofiber nerve guide according to claim 8, characterized in that: the aperture of the winding drum in the winding receiving device is 1mm-50cm, and the rotation speed is 200-10000rpm. 10.根据权利要求4所述的一种高取向纳米纤维神经导管的制备方法，其特征在于：步骤（2）中所述的制成神经导管为将干燥后的纤维支架在导管芯棒上卷绕成导管，所述的导管芯棒的直径为0.01mm～10cm。10. The preparation method of a highly oriented nanofiber nerve guide according to claim 4, characterized in that: the preparation of the nerve guide described in step (2) is to roll the dried fiber scaffold on the guide mandrel Wound into a catheter, the diameter of the catheter mandrel is 0.01 mm to 10 cm.
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103432628A (en) * 2013-07-22 2013-12-11 山东省立医院 Making method of carbon nanotube reinforced composite material nerve conduit
CN103751839A (en) * 2013-12-17 2014-04-30 中国人民解放军第二军医大学 Polylactic acid-chitosan composite nerve conduit and preparation method thereof
CN103877612A (en) * 2014-04-01 2014-06-25 大连医科大学附属第一医院 Cell stent with carbon nano tube and preparation method thereof
CN103981590A (en) * 2014-05-07 2014-08-13 重庆大学 Method for preparing PCL micro-nano doublet fibers through one-step emulsification electrospinning
CN104027842A (en) * 2014-06-20 2014-09-10 东华大学 Preparation method of axially oriented nanofiber nerve conduit
CN106668938A (en) * 2017-03-06 2017-05-17 山东赛克赛斯生物科技有限公司 Nerve conduit and preparation method thereof
CN108048943A (en) * 2018-01-15 2018-05-18 苏州大学 Highly oriented porous nano-fibre and preparation method thereof
CN108653806A (en) * 2018-05-04 2018-10-16 北京化工大学 A kind of multichannel conduction nerve rehabilitating tube and preparation method thereof with fluorescent characteristic
CN109758617A (en) * 2019-03-11 2019-05-17 宁波光远致信生物科技有限公司 A kind of neural restoration film and its preparation method and application
CN110219096A (en) * 2019-07-10 2019-09-10 福州大学 A kind of preparation method of PCL/CNTs composite electrostatic spinning film

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005045122A1 (en) * 2003-11-04 2005-05-19 Drexel University Electrospun carbon nanotube reinforced silk fibers
WO2006105478A2 (en) * 2005-03-31 2006-10-05 New York University Conducting polymer nanowire brain-machine interface systems and methods
CN101543645A (en) * 2009-05-04 2009-09-30 东华大学 Polycaprolactone (PCL) static spinning nerve conduit and preparation and application thereof
CN101912318A (en) * 2010-07-30 2010-12-15 东华大学 A three-layer electrospun ordered fiber nerve conduit and its preparation and application
CN102000363A (en) * 2010-11-10 2011-04-06 东华大学 Preparation method of CA/CS/CNTs (cellulose acetate/chitosan/ carbon nano tubes) composite nano fiber with good biocompatibility
CN102274510A (en) * 2011-07-15 2011-12-14 华南理工大学 Preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005045122A1 (en) * 2003-11-04 2005-05-19 Drexel University Electrospun carbon nanotube reinforced silk fibers
WO2006105478A2 (en) * 2005-03-31 2006-10-05 New York University Conducting polymer nanowire brain-machine interface systems and methods
CN101543645A (en) * 2009-05-04 2009-09-30 东华大学 Polycaprolactone (PCL) static spinning nerve conduit and preparation and application thereof
CN101912318A (en) * 2010-07-30 2010-12-15 东华大学 A three-layer electrospun ordered fiber nerve conduit and its preparation and application
CN102000363A (en) * 2010-11-10 2011-04-06 东华大学 Preparation method of CA/CS/CNTs (cellulose acetate/chitosan/ carbon nano tubes) composite nano fiber with good biocompatibility
CN102274510A (en) * 2011-07-15 2011-12-14 华南理工大学 Preparation method of carbon nanotube-chitosan-phycocyanin nanoparticles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
唐圣奎等: "多壁碳纳米管/ 聚己内酯超细复合纤维的制备及性能", 《复合材料学报》 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103432628A (en) * 2013-07-22 2013-12-11 山东省立医院 Making method of carbon nanotube reinforced composite material nerve conduit
CN103432628B (en) * 2013-07-22 2016-07-06 山东省立医院 The preparation method of carbon-nanotube enhanced composite nerve trachea
CN103751839A (en) * 2013-12-17 2014-04-30 中国人民解放军第二军医大学 Polylactic acid-chitosan composite nerve conduit and preparation method thereof
CN103751839B (en) * 2013-12-17 2015-10-28 中国人民解放军第二军医大学 A kind of polylactic acid and chitosan composite nerve conduit and preparation method thereof
CN103877612A (en) * 2014-04-01 2014-06-25 大连医科大学附属第一医院 Cell stent with carbon nano tube and preparation method thereof
CN103877612B (en) * 2014-04-01 2016-03-09 大连医科大学附属第一医院 Cytoskeleton of a kind of carbon nanotubes and preparation method thereof
CN103981590A (en) * 2014-05-07 2014-08-13 重庆大学 Method for preparing PCL micro-nano doublet fibers through one-step emulsification electrospinning
CN104027842A (en) * 2014-06-20 2014-09-10 东华大学 Preparation method of axially oriented nanofiber nerve conduit
CN106668938A (en) * 2017-03-06 2017-05-17 山东赛克赛斯生物科技有限公司 Nerve conduit and preparation method thereof
CN106668938B (en) * 2017-03-06 2019-12-06 赛克赛斯生物科技股份有限公司 Nerve conduit and preparation method thereof
CN108048943A (en) * 2018-01-15 2018-05-18 苏州大学 Highly oriented porous nano-fibre and preparation method thereof
CN108048943B (en) * 2018-01-15 2024-02-23 苏州大学 Highly oriented porous nanofibers and method of making the same
CN108653806A (en) * 2018-05-04 2018-10-16 北京化工大学 A kind of multichannel conduction nerve rehabilitating tube and preparation method thereof with fluorescent characteristic
CN108653806B (en) * 2018-05-04 2021-04-06 北京化工大学 Multi-channel conductive nerve repair catheter with fluorescence characteristic and preparation method thereof
CN109758617A (en) * 2019-03-11 2019-05-17 宁波光远致信生物科技有限公司 A kind of neural restoration film and its preparation method and application
CN109758617B (en) * 2019-03-11 2022-06-03 宁波光远致信生物科技有限公司 Nerve repair membrane and preparation method and application thereof
CN110219096A (en) * 2019-07-10 2019-09-10 福州大学 A kind of preparation method of PCL/CNTs composite electrostatic spinning film

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Application publication date: 20120919