CN102600967A - Intelligent control magnetic separator - Google Patents

Abstract

The invention relates to an intelligent control magnetic separator, comprising a magnet (1) and a sample pool (2), characterized in that there are a monochromatic light generator (3) and a monochromatic light generator (3) on one side of the sample pool (2). ) is connected with a wavelength selector (4), the corresponding side of the sample cell is equipped with a photoelectric detector (5), and the photodetector (5) is connected with a signal processing and display system (6); the magnet (1) is close to the sample pool (2), the monochromatic light generator (3) emits monochromatic light, after the wavelength is selected by the wavelength selector (4), the light of a single specific wavelength is shot through the sample pool (2), and the light passes through the photoelectric detector ( 5) Quality testing, and then display by signal processing and photoacoustic alarm system (6). The present invention does not need to observe the separation of the sample with the naked eye, but can directly observe the separation effect through the display screen, and an alarm signal is sent by the alarm system to indicate that the separation is completed, which is very convenient to use.

Description

An Intelligently Controlled Magnetic Separator

technical field

The invention belongs to the field of separation equipment, in particular to a magnetic separator.

Background technique

Magnetic bead separation technology is a new separation method based on surface functionalized magnetic beads (Magnetic Beads, MBs) as the separation medium. Magnetic beads are usually made of superparamagnetic nano-ferric oxide as the core and wrapped by non-magnetic polymer materials. Targets can be captured specifically or non-specifically by modifying different biological and chemical functional groups on the surface of magnetic beads. This method combines the simplicity of magnetic bead separation with the high efficiency of dispersed capture of micro-nano particles (large specific surface area), adopts the separation mode combined with magnetic beads, and overcomes the shortcomings of traditional separation operations (such as compared with column separation, based on The separation of the column principle takes a long time, is easy to block, the separation efficiency will decrease if the flow rate is too fast, and the short circuit caused by the uneven packing of the packing when packing the column); at the same time, because the particle size of the magnetic beads is between nanometers and submicrons Between, the specific surface area is much larger than the solid phase material, the amount of magnetic beads is small, the enrichment multiple is high, and the adaptability to the matrix is strong; the magnetic beads have good dispersibility in the solution, can be evenly distributed in the solution, and the specific surface area is large. It improves the rate of capturing isolates in the separation process and shortens the capture time; at the same time, it can process large-volume samples in a short time, which is beneficial to increase the enrichment factor and reduce the detection limit by increasing the sample processing volume.

After the magnetic beads are combined with the sample, a magnetic separator is commonly used to absorb the magnetic beads bound to the target object on the magnetic separator, and then separate it from the solution. Through multiple washings and elutions, a high concentration of the target object is obtained to achieve The effect of enriching the target substance.

However, in the process of use, the separation effect of the magnetic separator needs to be judged by the naked eye, which is very troublesome, with large errors and poor effect.

Contents of the invention

Aiming at the disadvantage of needing to observe the separation effect of the magnetic separator with the naked eye, the present invention provides a magnetic separator capable of detecting the separation effect.

The specific plan is:

The magnetic separator includes a magnet, a sample cell, a monochromatic light generator and a wavelength selector on one side of the sample cell, a photoelectric detector on the corresponding side of the sample cell, a signal processing display and a photoacoustic alarm system; the magnet is close to the sample cell. The monochromatic light generator emits monochromatic light, which is selected by the wavelength selector to form a single specific wavelength light that passes through the sample cell, is absorbed by the photoelectric detector, and then displayed by the signal processing and display system. After signal processing and display, the change of OD value of the solution in the sample cell can be observed, so as to know whether the sample is completely separated.

In order to achieve better separation and detection results, the sample cell is a cylinder with a cross-section of a circle, an ellipse, or a polygon with a pair or more of parallel sides. This allows light to pass through the center of the cross section without being blocked by the magnets. The cross-section is circular, monochromatic light passes through the center of the circle, and the light can be directly incident on the photoelectric detector without refraction of the interface; The cross-section is square, and the monochromatic light is parallel to the side of the square and passes through the center of the square, and hits the photodetector without interface refraction; the cross-section has a pair or more A polygon with sides, such as a regular hexagon, the light passes through the center and is perpendicular to the parallel sides; the light passes through the center of the cross-section of the sample cell, and the magnets are arranged close to the edge of the sample cell so as not to block the light from passing through the sample cell The center of the cross section shall prevail.

In order to achieve a better separation effect, the magnetic separator contains multiple magnets, the shape of which is suitable for the sample cell, and it is also close to the sample cell, and the placement position should not block the light.

During sample separation, it is often necessary to adjust the temperature of the sample in the sample cell. There is also a temperature control device at the bottom of the magnetic separator, which can adjust the temperature of the sample cell by heating and cooling, so that the sample can be separated better. The temperature control device can be an ordinary heating and temperature regulating device. It stops heating or cooling after the temperature reaches the set value. It can be placed at the bottom of the sample pool or inside the shell of the magnetic separator. Can. Typically, magnetic separators can have an enclosure to protect the magnetic separator components.

The beneficial effects of the present invention are:

1. There is no need to observe the separation of the sample with the naked eye, and the separation effect can be directly observed through the display screen, which is very convenient.

2. The separation effect is displayed by the change of absorption value, and the result is accurate, which is conducive to precise control.

3. The structure is simple and very easy for industrialized production.

Description of drawings

Figure 1 The sample cell is a square magnetic bead separation device.

Figure 2 The sample cell is a circular magnetic bead separation device.

Figure 3 The sample cell is a diamond-shaped magnetic bead separation device.

Figure 4 The sample cell is a regular hexagonal magnetic bead separation device.

Detailed ways

Example 1

It can be seen from accompanying drawing 1 that the magnetic separator has a sample cell for holding samples, a monochromatic light generator and a wavelength selector are arranged on one side of the sample cell, and a photoelectric detector is arranged on the corresponding side of the sample cell and the monochromatic light generator. and signal processing display system. The monochromatic light generator emits monochromatic light, which is selected by the wavelength selector to form a single specific wavelength light that passes through the sample cell, is absorbed by the photoelectric detector, and then displayed by the signal processing and display system. After signal processing and display, the change of OD value of the solution in the sample cell can be observed, so as to know whether the sample is completely separated. In order to improve the adsorption capacity, permanent magnets are placed close to the upper and lower sides of the sample cell, so that the magnetic beads can be quickly adsorbed by the magnets. There is also a temperature control device at the bottom of the sample pool. When the temperature control device senses that the temperature in the magnetic separator is too low or too high, it will heat up or cool down to the set temperature, so that the sample can be kept at an appropriate temperature, which is beneficial Maintain sample properties and improve separation efficiency.

Accompanying drawing 2 is a cylindrical sample cell, and its cross-sectional shape is circular.

Accompanying drawing 3 is a rhombus column sample cell, and its cross-sectional shape is rhombus.

Accompanying drawing 4 is a polyhedral column sample cell, and its cross-sectional shape is a regular hexagon.

In order to achieve a better effect, the sample cell is a cylinder with a cross section of a circle, an ellipse, or a polygon with a pair or more of parallel sides. This allows light to pass through the center of the cross section and not be blocked by the magnets. The cross-section is circular, and the monochromatic light passes through the center of the circle, so that the light can be directly incident on the photoelectric detector without refraction at the interface; the cross-section is elliptical, and the monochromatic light is incident on the There will be no interface refraction on the photodetector; the cross section is a square, and the monochromatic light is parallel to the side of the square and passes through the center of the square, and it will shoot on the photodetector without interface refraction; the cross section is a pair or more than a polygon with parallel sides, such as a regular hexagon, the light passes through the center and is perpendicular to the parallel sides; the light passes through the center of the cross-section of the sample cell, and the magnets are arranged close to the edge of the sample cell so as not to block the light from passing through. The center of the cross-section of the sample cell shall prevail.

Claims (5)

1. Magnetic separator, including magnet (1), sample cell (2), characterized in that there is a monochromatic light generator (3) on one side of the sample cell (2), and the monochromatic light generator (3) is connected with a wavelength selection There is a photoelectric detector (5) on the corresponding side of the sample cell, and a signal processing display system (6) is connected to the photoelectric detector (5); the magnet (1) is close to the sample cell (2), The monochromatic light generator (3) emits monochromatic light, and after the wavelength is selected by the wavelength selector (4), the light of a single specific wavelength is shot through the sample cell (2), and the light is detected by the photoelectric detector (5). Then it is displayed by the signal processing display and the photoacoustic alarm system (6). 2. The magnetic separator according to claim 1, characterized in that the sample cell (2) is a cylinder with a circular, elliptical, or polygonal cross-section with one or more parallel sides; the light passes through the sample cell (2) Center of cross section. 3. The magnetic separator according to claim 1, characterized in that it comprises a plurality of magnets (1) close to the sample cell (2), and the shape of the magnet is adapted to the sample cell. 4. The magnetic separator according to claim 1, characterized in that the magnet (1) is a permanent magnet. 5. The magnetic separator according to any one of claims 1-4, characterized in that there is a temperature control device (7) at the bottom of the sample pool (2).

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