CN101692999A - Mouth wash containing grape pip procyanidin extract and application - Google Patents

Abstract

The invention provides mouth wash containing a grape pip procyanidin extract, which is prepared from the grape pip procyanidin extract and a common mouth wash component by a traditional preparation process. The pH value of the mouth wash is 5.5-7.5, wherein the grape pip procyanidin extract accounts for 0.1-30% of the total amount in percent by weight, and the content of procyanidin in the grape pip procyanidin extract is not less than 95%. The provided mouth wash containing the grape pip procyanidin extract is used as a daily health-care nursing product of the oral cavity for daily use, can prevent dental caries and parodontitis and also can be used as an auxiliary treatment medicine for a dentist to treat caries and parodontopathy.

Description

Mouthwash containing grape seed proanthocyanidin extract and its application

technical field

The invention belongs to a health care product and relates to a mouthwash containing grape seed proanthocyanidin extract, which plays a role in the prevention and control of dental caries and periodontal disease and the technical field of daily oral care and health care.

Background technique

Mouthwash, as an oral health care product, has been widely used in people's daily life and oral adjuvant therapy. It can prevent and control caries and periodontal disease, and has a good effect on common oral diseases such as toothache, gingivitis, and bleeding gums. , has obvious heat-clearing, anti-inflammatory, hemostatic, antibacterial, analgesic effects, and removes bad breath.

Caries and periodontal disease are the most common oral diseases, and the research on these two diseases has always been the focus of stomatology research in my country. The theory of caries ecology believes that the bacteria related to the occurrence of caries are all normal oral microflora. Under physiological conditions, the bacteria and the host are in a dynamic balance, and no disease occurs; Changes in food factors lead to changes in the oral ecological environment, the physiological combination between normal bacteria and the host changes into a pathological combination, the oral ecological balance is transformed into dysbiosis, and normal bacteria become conditional pathogens, causing dental caries Streptococcus is the oral streptococcus most closely related to caries.

The source of natural medicine is wide, and the toxic and side effects are small. The development and development of natural herbal medicine is a key point in the current medicine and food research. For more than 100 years, grapes have been the subject of enduring research among many plants. Since the 1950s, people have isolated and identified polyphenolic compounds, fatty acids, vitamins, enzymes, carbohydrates, amino acids, polypeptides and proteins, terpenes and volatile oil components, as well as lipids, pectin and waxes from grape fruits, leaves and other parts Among the many ingredients, the medical efficacy of grapes is mostly related to the pharmacological activity of proanthocyanidins. As a source of proanthocyanidins, grape seed proanthocyanidin extracts have received the most in-depth and extensive attention. Many studies at home and abroad have found that its It has a variety of important biological activities and pharmacological effects, has strong anti-oxidation and free radical scavenging activities, and can resist cardiovascular diseases through various mechanisms such as protecting vascular endothelial function, resisting myocardial oxidative stress damage and inhibiting the oxidation of low-density lipoprotein. Disease has become a research hotspot in recent years.

Bacterial experiments and animal experiments have found that grape seed proanthocyanidins extract has an inhibitory effect on Streptococcus mutans. Some foreign scholars have suggested that grape seed proanthocyanidin extract has the effect on the production of reactive oxygen species and reactive nitrogen in mice, as well as the expression of inducible nitric oxide synthase stimulated by lipopolysaccharide macrophages in periodontal disease mice. Seed proanthocyanidin extract can be considered as a drug development for the prevention of periodontal disease.

Contents of the invention

The invention provides a mouthwash containing grape seed proanthocyanidin extract, which is mixed with ethanol, medicinal glycerin, natural peppermint oil, additives (edible flavor, sweetener), deionized water, etc., Filtration to obtain a clear, transparent light reddish-brown liquid with a pH value of 5.5-7.5, wherein the grape seed proanthocyanidin extract accounts for 0.1%-30% of the total amount by weight percentage,

The main components are as follows in proportion by weight:

Grape Seed Proanthocyanidins Extract 0.1～30%

95% ethanol 0.1～0.4%

Medicinal glycerin 0.5～5%

Natural peppermint oil 0.03～0.2%

Additives (flavors, sweeteners) 0.1～0.4%

Deionized water is mixed to 100%.

Grape seed proanthocyanidin extract is obtained by drying and crushing grape seeds, separating the kernel shell, extracting with ethanol in the shell, filtering, and spray drying to obtain a crude extract. The crude extract is dried by solvent fractionation or activated carbon adsorption to obtain red Brown powder.

Another object of the present invention is to provide the application of the mouthwash containing grape seed proanthocyanidin extract in the preparation of adjuvant medicines for preventing dental caries and periodontitis.

The present invention effectively utilizes the active ingredient proanthocyanidins in the grape seed extract, and adopts a preparation process to prepare a mouthwash containing grape seed proanthocyanidin extract, which has preventive and control effects on common dental caries and periodontal diseases, and can effectively kill Kill Streptococcus mutans, affect the progress of periodontitis, not only can be used for adjuvant treatment of common oral diseases, but also for daily oral health care.

Detailed ways

The present invention is described further in conjunction with embodiment.

Example 1

The preparation of the mouthwash containing grape seed proanthocyanidin extract is first to extract the grape seed proanthocyanidin extract, that is, the grape seed is dried and pulverized, and after the kernel and shell are separated, the crude extract is obtained by extracting in the shell with ethanol, filtering, and spray drying. The crude extract is dried by solvent fractionation or activated carbon adsorption to obtain a reddish-brown powder. Then mix the grape seed proanthocyanidin extract with ethanol, medicinal glycerin, natural peppermint oil, additives (edible flavors, sweeteners), deionized water, etc., and filter to obtain a clear, transparent and light-colored product with a pH value of 5.5 to 7.5. Reddish-brown liquid, the main components are as follows by weight:

Grape Seed Proanthocyanidins Extract 0.1～30%

95% ethanol 0.1～0.4%

Medicinal glycerin 0.5～5%

Natural peppermint oil 0.03～0.2%

Additives (flavors, sweeteners) 0.1～0.4%

Deionized water mixed to 100%

Wherein the proanthocyanidin content in the grape seed proanthocyanidin extract is more than 95%, and the additives are edible spices and sweeteners.

The mouthwash containing grape seed proanthocyanidin extract is used as a daily oral health care product to prevent dental caries and periodontitis, and can also be used by stomatologists as an auxiliary treatment for caries and periodontal disease.

Example 2

The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of grape seed proanthocyanidin extract (GSPE) against Streptococcus mutans were determined by bacterial experiments. Select the international standard strains, Streptococcus mutans (S.mutans Ingbritt, serotype c) and Streptococcus farreus (S.Sobrinus 6715, serotype g) of the Streptococcus mutans family, and digest soybean liquid with 1% sucrose in trypsin Anaerobic culture (CO ₂ 80%, N ₂ 10%, H ₂ 10%) in culture medium (TSB), using liquid/agar dilution method in vitro antibacterial test to determine the effect of grape seed proanthocyanidin extract (GSPE) on the two mutants. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of streptobacter were MIC 4.00mg/ml and MBC 8.00mg/ml respectively.

Example 3

The anti-caries effect of grape seed proanthocyanidin extract (GSPE) was further confirmed by animal experiments. Select 16 weaned Sprague-Dawby (SD) rats at the age of 21 days, and randomly divide them into 2 groups, 8 rats in each group, half male and half male, respectively grape seed proanthocyanidin extract (GSPE) solution and distilled water (H ₂ O) group . During the period of 21 to 23 days, the cariogenic feed 2000# with ancient broad-spectrum antibiotics (2 kg feed plus 1 g of ampicillin and 1 g of chloramphenicol) was given, and no antibiotics were added to the feed at other times. During the age of 24-28 days, the rats were inoculated with Streptococcus mutans S. Sobrinus 6715 ( ^{10 5-6} CFU/ml) in the oral cavity for 5 consecutive days, 100ul each time. From 29 days onwards, the two groups were treated with GSPE solution with MIC concentration of 4 mg/ml and distilled water. The rats were placed in a supine position, and 0.3ml of the drug was injected into the tooth surface and oral cavity of the rats with a blunt needle syringe for 15 seconds, once a day, and food and drink were prohibited within 30 minutes after the treatment. After continuous treatment for 60 days, the rats were sacrificed, and the caries condition of rat molar occlusal surface was observed and evaluated by Keyes dental caries scoring method. SPSS 13.0 statistical software was used for one-way analysis of variance and mean comparison, and the effect of GSPE on the Sprague-Dawley scale was summarized. Preventive effect of pit and fissure caries on the occlusal surface of molars in rats. Animal experiments found that GSPE can inhibit the occurrence and development of caries in rats (P<0.05); Dx grade caries was only found in the distilled water group, and no Dx grade caries occurred in the grape seed proanthocyanidin extract (GSPE) group.

Claims (4)

1. a collutory that contains grape pip procyanidin extract is characterized in that, each component is by weight:

Grape pip procyanidin extract 0.1～30%

95% ethanol 0.1～0.4%

Medicinal glycerin 0.5～5%

Natural menthence 0.03～0.2%

Additives 0.1～0.4%

Deionized water is assigned to 100%,

PH value is 5.5～7.5.

2. a kind of collutory that contains grape pip procyanidin extract according to claim 1 is characterized in that additives are flavorant or sweeting agent.

3. a kind of collutory that contains grape pip procyanidin extract according to claim 1, it is characterized in that, described grape pip procyanidin extract is to be pulverized by the Semen Vitis viniferae drying, after the core shell separates, with ethanol in shell, extract, filtration, spray drying get crude extract, crude extract is through solvent staging or dry bronzing powder, procyanidin content in the grape pip procyanidin extract 〉=95% of getting of active carbon adsorption.

4. a kind of application of collutory in preparation auxiliary treatment caries prevention and periodontitis medicine that contains grape pip procyanidin extract according to claim 1.