CN101133785A - A kind of production method of concentrated barley wort - Google Patents
A kind of production method of concentrated barley wort Download PDFInfo
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- CN101133785A CN101133785A CNA2007100171931A CN200710017193A CN101133785A CN 101133785 A CN101133785 A CN 101133785A CN A2007100171931 A CNA2007100171931 A CN A2007100171931A CN 200710017193 A CN200710017193 A CN 200710017193A CN 101133785 A CN101133785 A CN 101133785A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 240000005979 Hordeum vulgare Species 0.000 title 1
- 241000209219 Hordeum Species 0.000 claims abstract description 58
- 238000000034 method Methods 0.000 claims abstract description 21
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 11
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical group OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 3
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- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 2
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- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 2
- 101710094661 Beta-amylase 8 Proteins 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- LUEWUZLMQUOBSB-UHFFFAOYSA-N UNPD55895 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(O)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O LUEWUZLMQUOBSB-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- UYQJCPNSAVWAFU-UHFFFAOYSA-N malto-tetraose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(CO)O1 UYQJCPNSAVWAFU-UHFFFAOYSA-N 0.000 description 1
- LUEWUZLMQUOBSB-OUBHKODOSA-N maltotetraose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O[C@@H]3[C@@H](O[C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-OUBHKODOSA-N 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 150000004043 trisaccharides Chemical class 0.000 description 1
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- Non-Alcoholic Beverages (AREA)
Abstract
本发明公开了一种浓缩大麦麦汁的生产方法，它是以大麦为原料，采用脱壳去皮、粉碎调浆、预水解、喷射连续液化、两段糖化、过滤、浓缩、罐装一系列步骤完成的，在预水解工序中添加了以耐高温α－淀粉酶、蛋白酶、β－葡聚糖酶组成的复合水解酶，在二段糖化工序中，第一段糖化添加了以中温α－淀粉酶、蛋白酶、β－葡聚糖酶、木聚糖酶、植酸酶组成的复合水解酶，第二段糖化添加了以β－淀粉酶、植酸酶组成的复合水解酶。本发明能实现大麦中可溶性蛋白质水解充分、植酸盐类物质得到分解、β－葡聚糖降解率高，大麦麦汁中的α－氨基氮和可溶性氮含量较高，所生产的产品不仅营养性高、口感好，而且完全符合啤酒酿造的要求。The invention discloses a production method of concentrated barley wort, which uses barley as raw material, adopts a series of methods of shelling and peeling, pulverizing and pulping, pre-hydrolysis, spraying continuous liquefaction, two-stage saccharification, filtration, concentration and canning. After the steps are completed, a compound hydrolase composed of high-temperature-resistant α-amylase, protease, and β-glucanase is added in the pre-hydrolysis process. In the second-stage saccharification process, a medium-temperature α- A compound hydrolase composed of amylase, protease, β-glucanase, xylanase, and phytase, and a compound hydrolase composed of β-amylase and phytase is added in the second stage of saccharification. The invention can realize sufficient hydrolysis of soluble protein in barley, decomposition of phytate substances, high degradation rate of β-glucan, high content of α-amino nitrogen and soluble nitrogen in barley wort, and the produced products are not only nutritious High stability, good taste, and fully meet the requirements of beer brewing.
Description
Technical field
The present invention relates to a kind of production method of concentrated barley juice, particularly a kind of production method of brewing barley wheat juice.
Background technology
With barley is the concentrated barley juice that raw material (rather than fructus hordei germinatus) is produced by enzyme engineering technology, is a kind of auxotype syrup with nutrition, fragrance, can be widely used in the production and processing of food such as cake, candy, beverage.At present to be with starch be the syrup that raw material is produced to the used syrup of these food service industrys, composition single (100% starch sugar), and the food nutrition that adopts this syrup to process is complete, do not have characteristic and local flavor.
China's beer volume of production and marketing develops rapidly and becomes the first in the world production and marketing big country.At present, the wheat juice that brewing is used, be to make fructus hordei germinatus through a series of courses of industrialization such as immersion, germination, oven dry earlier with the quality beer barley, and then obtain through pulverizing, saccharification, process such as boil, this technical process overall complicated, water consumption are big, the active principle loss can reach more than 10%, more distinct issues are that China mainly relies on import quality beer barley to satisfy the wine brewing needs, though and the domestic barley price is low, exist a lot of problems such as germination percentage is low and irregular, substance decomposition is not thorough traditionally.Therefore, with homemade vulgare is that raw material adopts enzyme engineering method production brewing can be expected to overcome the deficiency of domestic barley with wheat juice without germination process, thereby obtained paying attention to and research, but the production method of existing open report, only do not rest on laboratory or pilot scale stage, and the protein that causes because of the defective of its process decomposes insufficient, AAN is obviously not enough, the beta glucan decomposition does not thoroughly wait brewing and the serious problem of beer quality influence is not solved at all yet, this has not only reduced the trophism of barley juice, and is used for brewing and also is restricted.
Summary of the invention
The objective of the invention is to want to overcome above-mentioned the deficiencies in the prior art, a kind of production method of new concentrated barley juice is provided, adopt this method to produce barley juice, can realize that the soluble protein hydrolysis is abundant in the barley, the phytic acid salts substances obtains decomposing, beta glucan degradation rate height, AAN in the barley juice and soluble nitrogen content are higher, the product of being produced not only trophism height, mouthfeel is good, and meets the requirement of brewing fully.
For realizing that the technical scheme that above-mentioned purpose of the present invention adopts is: a kind of production method of concentrated barley juice is a raw material with the barley, it is characterized in that being undertaken by following step:
1, shelling peeling: adopt barley huller to remove shell sclerderm and other foreign material of barley;
2, pulverizing is sized mixing: the pearling cone meal after will shelling is broken into the following powder of 60 orders, adds 3-3.5 doubly to the water that meets the food standard for drinking of powder, adjusts pH between 6.0-6.5, and the formation feed liquid pumps into feed liquid in the prehydrolysis jar then;
3, prehydrolysis: in the prehydrolysis jar, in feed liquid, add the composite hydrolytic enzyme A of 500-1000 gram, after stirring, keep carrying out in 0.5-1 hour prehydrolysis after being warming up to 45-55 ℃ by powder per ton;
4, spray liquefaction continuously: with the high steam is thermal source, adopts Jet liquefier and liquefaction process continuously, and the temperature control is kept the feed liquid after the prehydrolysis and liquefied continuously in 0.5-1 hour in 100-108 ℃ of scope;
5, two sections saccharification: the feed liquid after at first will liquefying cools to 5 ℃ of 45-5, adjust pH is 6.0-6.5, adds the composite hydrolytic enzyme B that 600-1200 restrains by powder per ton, keeps 1.5-2 hour, finishes first section saccharification; Adjust feed temperature then and be 55-65 ℃, pH value and be 5.5-5.0, add the composite hydrolytic enzyme C of 1000-1500 gram, kept 3-5 hour, finish second section saccharification by powder per ton;
6, filter: will filter through the feed liquid after two sections saccharification, the filtrate of acquisition is the barley juice that dry matter content is 18-20%;
7, concentrate: barley juice is condensed into the high concentration barley juice that dry matter content is 78-82%;
8, canned: as to adopt the packing tube that meets the packaging for foodstuff requirement, can under sanitary condition, envelope storage;
Contain among every gram composite hydrolytic enzyme A: high temperature resistant AMS 5000-10000 enzyme live unit, a protease 25000-40000 enzyme live unit, a 1,4 beta-glucanase 200-500 enzyme work unit;
Contain among every gram composite hydrolytic enzyme B: 100-300 enzyme of middle temperature AMS unit alive, protease 25000-40000 enzyme unit alive, 1,4 beta-glucanase 200-500 enzyme unit alive, zytase 2000-5000 enzyme unit alive, phytase 100-200 enzyme unit alive;
Contain among every gram composite hydrolytic enzyme C: β--amylase 8 0000-100000 enzyme live unit, a phytase 100-150 enzyme work unit.
In the described enrichment process, most preferred embodiment is for adopting two sections to concentrate: adopting multi-effect evaporator is that the barley juice of 18-20% is concentrated to 40-60% with dry matter content, finish elementary concentrate, and then adopt rotor segment formula evaporimeter barley juice to be concentrated into the high concentration barley juice that dry matter content is 78-82% being lower than under 70 ℃.
Adopt method of the present invention to produce concentrated barley juice, the technical process and the process conditions of the concentrated barley juice of suitability for industrialized production have clearly been solved, particularly prehydrolysis, two sections saccharification two procedures and the plurality of enzymes preparation that is adopted, increased the decomposition of phytic acid material in the barley, guaranteed the hydrolysis requirement of each material in the barley, thereby realized that the soluble nitrogen hydrolysis is thorough in the barley juice, the soluble protein hydrolysis is abundant, comparatively rationally (the A district is 8-12% to lundin fraction, the B district is 20-25%, the C district is 60-70%), AAN (mg/L) and soluble nitrogen are all from the hydrolysis of barley itself, and AAN and soluble nitrogen content are higher, can reach 180 mg/L and 990mg/L above (by 12 ° of p wheat juice conversions) respectively, this index is inaccessible in disclosed method; Its beta glucan degradation rate reaches more than 90%, the starch percent hydrolysis reaches 100%, hydrolysate glucose is 5-10%, maltose is 45-55%, maltotriose is 15-20%, the above compound sugar of maltotetraose level is 20-25%, such index is the trophism height not only, mouthfeel is good, improved the nutrition degree of barley juice, and meet the requirement of brewing fully, simultaneously, the concentrate of this wheat juice also is a kind of good auxotype raw-food material, because the concentrated barley juice (syrup) that adopts this method to produce, compare with the syrup that with starch is raw material production, not only contain same saccharic composition, and contain more protein hydrolysate nutriments such as (comprising amino acids), the existence of these materials, not only increased trophism, and in food processing, the Mei Lande reaction has easily taken place and enrich the fragrance of food.
Adopt method production brewing of the present invention to reach the standard that fructus hordei germinatus that brewery adopts is produced wheat juice fully with its component of wheat juice, following table is the contrast between the two:
| The contrast project | Product of the present invention | The wheat juice that fructus hordei germinatus is produced |
| Product dry matter content (g/L) | Inspissated juice 78-80 | 12 |
| Glucose/total reducing sugar (%) maltose/total reducing sugar (%) maltotriose/total reducing sugar (%) fermentable sugar/total reducing sugar (%) trisaccharide is above/and total reducing sugar (%) colourity (being diluted to 12P/EBC) final attenuation (%) AAN is (by 12 ° of p, mg/L) the solubility total nitrogen is (by 12 ° of p, mg/L) macromolecule nitrogen/total nitrogen (%) macromolecule nitrogen/total nitrogen (%) macromolecule nitrogen/total nitrogen (%) | 8.31 52.75 17.23 71.29 28.71 3-4 66.3 184.4 994.6 12.56 24.04 63.4 | About≤10 43～53 10～20 70～75 15～35 3-5 65～70 180～200 800～1200＜25 15～25 60 |
The specific embodiment
Below in conjunction with the concrete example of implementing the present invention is done further detailed description.
1, shelling peeling: selecting barley for use is raw material, adopts barley huller to remove shell sclerderm and other foreign material of barley, require husking yield at 70-90%, must not destroy seed.
2, pulverize and to size mixing: the barley after will shelling (can add 5-10% baking after burnt odor barley) is ground into powder below 60 orders with beater grinder, add 3-3.5 doubly to the water that meets the food standard for drinking of powder, adjust pH between 6.0-6.5 with edible soda acid, form feed liquid, then feed liquid is pumped in the prehydrolysis jar.
3, prehydrolysis: in the prehydrolysis jar, add the composite hydrolytic enzyme A of 500-1000 gram, after stirring, be warmed up to 45-55 ℃, keep carrying out in 0.5-1 hour prehydrolysis by powder per ton.
4, spray liquefaction continuously: with the high steam is thermal source, adopts Jet liquefier and continuous liquefaction process, and the control temperature liquefied the feed liquid after the above-mentioned prehydrolysis 0.5-1 hour in 100-108 ℃ of scope continuously.
5, two sections saccharification: the feed liquid after at first will liquefying cools to 45-55 ℃, adjust pH to 6.0-6.5, adds the composite hydrolytic enzyme B of 600-1200 gram by powder per ton, keeps 1.5-2 hour, finishes first section saccharification; Adjust feed temperature then and be 55-65 ℃, pH value and be 5.5-5.0, add the composite hydrolytic enzyme C of 1000-1500 gram, kept 3-5 hour, finish second section saccharification by powder per ton.
6, filter: will filter through plate and frame filter press through the feed liquid after two sections saccharification, can obtain dry matter content is the barley juice filtrate of 18-20%.
7, two sections concentrate: adopting the falling film type triple effect evaporator is that the barley juice of 18-20% is concentrated to the barley juice that dry matter content is 40-60% with dry matter content, finish elementary concentrate, and then to adopt rotor segment formula evaporimeter be that the barley juice of 40-60% is concentrated into the high concentration barley juice that dry matter content is 78-82% with dry matter content.Concentrator is the colourity requirement that concentrates in conjunction with wheat juice, and the falling film type triple effect evaporator of generally use and the rotor segment formula of single-action are used in combination, and like this, has both realized the repeatedly use of steam, has reduced the look range degree of wheat juice again.
8, canned: as to adopt the packing tube that meets the packaging for foodstuff requirement, can under sanitary condition, envelope storage.
Employed composite hydrolytic enzyme A, composite hydrolytic enzyme B, composite hydrolytic enzyme C are composited by the live enzyme of unit of following enzyme in the operation
Composite hydrolytic enzyme A: calculating to make 1 gram composite hydrolytic enzyme A, is that the live solid enzyme powder amount of unit, protease 25000-40000 enzyme unit alive, 1,4 beta-glucanase 200-500 enzyme unit alive of high temperature resistant AMS 5000-10000 enzyme is evenly mixed promptly.
Composite hydrolytic enzyme B: calculating to make 1 gram composite hydrolytic enzyme B, is that 100-300 enzyme of middle temperature AMS live solid enzyme powder amount of unit, 1,4 beta-glucanase 200-500 enzyme unit alive, zytase 2000-5000 enzyme unit alive, phytase 100-200 enzyme unit alive of unit, a protease 25000-40000 enzyme of living is evenly mixed promptly.
Composite hydrolytic enzyme C: calculating to make 1 gram composite hydrolytic enzyme C, is with β--the live solid enzyme powder amount of unit, a phytase 100-150 enzyme work unit of amylase 8 0000-100000 enzyme evenly mixes promptly.
Product yield of the present invention: extract content in the products obtained therefrom/raw materials used middle extract content 〉=98%.
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| CNA2007100171931A CN101133785A (en) | 2007-09-26 | 2007-09-26 | A kind of production method of concentrated barley wort |
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| CNA2007100171931A CN101133785A (en) | 2007-09-26 | 2007-09-26 | A kind of production method of concentrated barley wort |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892135A (en) * | 2010-07-15 | 2010-11-24 | 大连工业大学 | A method for promoting germination of wheat |
| CN102805289A (en) * | 2011-06-01 | 2012-12-05 | 中粮集团有限公司 | Preparation method for concentrating corn steep liquor |
| WO2014008708A1 (en) * | 2012-07-12 | 2014-01-16 | 淮北酚醌健康咨询服务有限公司 | Treatment method for dissolving tumour and transforming cancer cells with phenol and quinone |
| CN103555484A (en) * | 2013-10-25 | 2014-02-05 | 华南理工大学 | Preparation method for ultrahigh-concentration wort |
| CN104328156A (en) * | 2014-10-27 | 2015-02-04 | 山东广博生物技术服务有限公司 | Method of liquefying high-concentration starch milk by enzymatic process |
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| CN103555484B (en) * | 2013-10-25 | 2015-09-16 | 华南理工大学 | A kind of preparation method of ultrahigh-concentratwort wort |
| CN104328156A (en) * | 2014-10-27 | 2015-02-04 | 山东广博生物技术服务有限公司 | Method of liquefying high-concentration starch milk by enzymatic process |
| CN107937291A (en) * | 2017-12-22 | 2018-04-20 | 广州南沙珠江啤酒有限公司 | A kind of wheat juice and its preparation process for yeast spread cultivation |
| CN107937291B (en) * | 2017-12-22 | 2021-06-04 | 广州南沙珠江啤酒有限公司 | Wheat juice for yeast propagation and preparation process thereof |
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| CN113150061A (en) * | 2021-04-21 | 2021-07-23 | 广州市合利源食品有限公司 | Barley malt extract produced by multi-enzyme synergistic process and production process thereof |
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