CN101066409A - A pharmaceutical composition for killing sperm and treating vaginitis - Google Patents

Abstract

The present invention provides one kind of medicine composition for killing sperm and treating vaginitis simultaneously. The medicine composition consists of active components comprising zedoary oil 0.5-20 wt% and hyodeoxycholic acid 0.5-20 wt% and pharmaceutically acceptable supplementary material. The medicine composition can kill sperm, change the physical and chemical properties of sperm and cervical mucus to prevent conception and kill microbes causing vaginal infection, so as to contracept and prevent and treat microbe caused viginitis.

Description

A pharmaceutical composition for killing sperm and treating vaginitis

technical field

The invention relates to the field of traditional Chinese medicines, in particular to traditional Chinese medicine compositions.

Background technique

In today's society, phenomena such as unplanned pregnancy and genitourinary tract infection have seriously affected the daily life of women and become one of the urgent problems in the medical field. At present, there are many methods of contraception. Condoms are currently the most common contraceptive method in the world, but their usage rate in developing countries is very low. In addition, because condoms can cause allergic reactions in the human body and affect pleasure, a considerable number of men resist their use. Contraceptives are widely used, mainly oral or injectable contraceptives and intravaginal drugs. Now tens of millions of people in the world are using oral contraceptives for women, but they have serious cardiovascular diseases, liver nephritis, endocrine diseases, tumors or Patients with blood diseases should not take it, as it may easily cause dizziness, nausea, vomiting, menstrual cycle disorders and other adverse reactions. The current use rate of Chinese women's oral contraceptives is on a downward trend. Female external contraceptives have dosage forms such as vaginal suppositories and vaginal diaphragms, but they are not accepted by many women because of complicated operations.

Since the 1990s, research on birth control technology has shifted from focusing solely on contraceptive effects to contraception, disease prevention and treatment. Spermicide, as one of the barrier contraceptive methods, has the dual functions of contraception and prevention of sexually transmitted diseases, and has the advantage that women can choose to use it independently. The currently commonly used surfactant contraceptives such as NP-9 have a certain damage effect on the vaginal epithelial tissue of women, and some will also affect the normal flora in the vagina. Therefore, the development of a compound Chinese medicine contraceptive that can not only prevent pregnancy, but also antibacterial and anti-inflammatory, and has little mucosal irritation has broad application prospects.

Zedoary oil is the extract of the rhizome of Zedoary. It has the effects of breaking blood, promoting qi and relieving pain, eliminating stagnation, breaking blood and removing stasis. Zedoary oil is widely used in the field of medicine, such as: compound zedoary oil suppository, each compound zedoary oil suppository contains 50mg of econazole nitrate, 0.21ml of zedoary oil and 3mg of borneol, used to treat various vaginal infections; zedoary oil injection , containing 1% zedoary oil, used for the treatment of early cervical cancer; compound zedoary oil soft capsule, the main active ingredients are zedoary oil and tangerine peel oil, used to treat stomach problems caused by indigestion. The effect of treating vaginitis with curcuma oil alone is not very satisfactory, so it is generally used in combination with antifungal agents to enhance its antibacterial and anti-inflammatory effects.

Hyodeoxycholic acid is a cholic acid monomer compound extracted from pig bile, which can inhibit the formation of cholic acid and dissolve fat, reduce blood cholesterol and triglyceride; etc. have a certain antibacterial effect and can be used as anti-inflammatory drugs; studies have shown that hyodeoxycholic acid also has a disintegrating effect on Trichomonas vaginalis cultured in vitro (Zhan Zhizeng, New Medicine, 1979, 10(2): 66) And strong spermicidal effect (Hu Qingfa et al., Shaanxi New Medicine, 1986, 15(8): 3).

Contents of the invention

The technical problem to be solved by the present invention is to provide a pharmaceutical composition for killing sperm and treating vaginitis, so as to further improve the antibacterial, anti-inflammatory and spermicidal effects of zedoary oil and hyodeoxycholic acid.

The technical scheme that the present invention realizes above-mentioned purpose is:

A pharmaceutical composition for killing sperm and treating vaginitis, the composition is composed of active ingredients and medically acceptable auxiliary materials, and is characterized in that it contains the following active ingredients in weight percent: zedoary oil 0.5-20%, hyodeoxycholic acid 0.5-20%; preferably turmeric oil 4%, hyodeoxycholic acid 2%.

Zedoary oil of the present invention is the dry rhizome of Zingiberaceae plant Curcuma phaeocaulis Valeton (Curcuma phaeocaulis Valeton), Wen Yujin (Curcumawenyujin YHChen et C.Ling) or Guangxi Zedoary (Curcuma kwangsiensis SGLee et C.F.Liang) etc. through steam distillation or The fat-soluble part extracted by supercritical carbon dioxide extraction technology; hyodeoxycholic acid is 3α, 6α-dihydroxy 5β-cholanic acid extracted from healthy pig bile, and the content of C ₂₀ H ₄₀ O ₄ should not be less than 98.0 %.

The pharmaceutical composition of the present invention is a clinically acceptable dosage form, such as lotion, ointment, gel, foam, suppository, aerosol or vaginal effervescent tablet. The adjuvant of described lotion is emulsifier and water, and wherein emulsifier selects Tween-80 for use; The adjuvant of described ointment is ointment base, humectant and emulsifier, wherein, ointment base selects carbomer for use, and moisturizer selects glycerol for use , emulsifier selects Tween-80 for use; The adjuvant of described gel is gel base, surfactant, humectant and emulsifier, wherein, gel base selects Poloxamer 407 for use, and surfactant selects methyl cellulose for use, moisturizing Glycerol is selected as the agent, and Tween-80 is selected as the emulsifier; the auxiliary materials of the foaming agent are emulsifier, humectant, preservative, acid-base neutralizer, plasticizer and surfactant, wherein the emulsifier is selected from Tween-80. 80. Glycerin is selected as the moisturizing agent, benzyl alcohol is selected as the preservative, triethanolamine is selected as the acid-base neutralizer, stearic acid is selected as the plasticizer, and flat plus O is selected as the surfactant; the auxiliary material of the suppository is the suppository base, such as cocoa soybean fat; the adjuvants of the aerosol are emulsifier, humectant, antiseptic, acid-base neutralizer, plasticizer, propellant and surfactant, wherein, emulsifier selects Tween-80 for use, and moisturizer selects Glycerin, antiseptic selects benzyl alcohol for use, acid-base neutralizer selects triethanolamine for use, plasticizer selects stearic acid for use, surfactant selects Pingping and O for use, and propellant selects dimethyl ether for use; Compounding agent and CO _Generating agent, wherein, inclusion agent selects cyclodextrin for use, CO _Generator selects boric acid, tartaric acid and sodium bicarbonate for use.

The active ingredient of the pharmaceutical composition of the present invention is composed of two traditional Chinese medicines, zedoary oil and hyodeoxycholic acid, and the zedoary oil is used as the king drug. "; 2. In the vagina, it can inhibit the survival rate of sperm, reduce the chance of conception, and achieve the contraceptive effect. With hyodeoxycholic acid as the ministerial drug, hyodeoxycholic acid mainly returns to the liver meridian, and is good at clearing damp-heat in the liver and gallbladder to eliminate pathogenic factors. The two medicines are used together to achieve the function of "clearing away heat and antibacterial, relieving pain and contraception". After compatibility, the antibacterial and anti-inflammatory effects are enhanced, and the effective spermicidal concentration is lower than that of hyodeoxycholic acid alone. If the "pruritus of vulvae" is exterminated, the symptoms of genital itching will be eliminated; if the excess heat in the liver and gallbladder is cleared, the amount of leucorrhea will be large, yellow in color, thick in quality, and smelly; Then sexual intercourse pain can be relieved.

In summary, the pharmaceutical composition of the present invention has two major advantages: first, as a preparation for vaginal administration, it not only has the effect of treating vaginitis, but also because the medicine monosodium glutamate is suitable, the effect is moderate, and it does not have irritation and corrosion. Simultaneously, it has therapeutic properties and practicality; secondly, the preparation can also reduce the survival rate of sperm in the vagina to achieve the purpose of contraception.

The advantages and attainable technical effects of the present invention will be further illustrated through experiments below.

1. Experiments on the spermicidal effect and contraceptive effect of the pharmaceutical composition of the present invention

(1) Test drug:

Pharmaceutical composition emulsion of the present invention: get pharmaceutical composition of the present invention, be mixed with turmeric oil 4%, hyodeoxycholic acid 2% and Tween-80 2% emulsion with 0.9% sodium chloride solution;

(2) Experimental materials:

Sperm: collected from adult healthy men (age 25-38 years old), abstinence for 3-7 days, masturbation method to collect semen in a clean test tube, liquefied within 0.5 hours at 37°C, routine inspection under light microscope, sperm density 50×10 ⁹ ～ 80×10 ⁹ L ^-1 , motility rate over 70%, motility IV, deformed sperm <30%, no sperm agglutination.

Cervical mucus: taken from a healthy adult ovulating woman, it has a stringiness, and fern-like crystals can be seen under the microscope after the smear is dried.

(3) Experimental method:

a. the effect of the composition of the present invention on seminal fluid: respectively add seminal fluid 0.5ml in 3 clean test tubes, add pharmaceutical composition emulsion 0.01ml, 0.02ml, 0.03ml of the present invention to these 3 tubes respectively in a certain interval time, Simultaneously, parallel control was performed with the semen that did not add the emulsion of the pharmaceutical composition of the present invention. Timing and shaking or blowing with a straw, and observed with the naked eye, pipette 20 μl of the mixed liquid in the test tube at 20 seconds, 1 minute, 15 minutes, 30 minutes, and 60 minutes after adding the drug solution, respectively, and put it on a glass slide, and observe under the microscope after adding a cover glass. Sperm motility, motility, density, etc. were recorded respectively.

b. The effect of the composition of the present invention on cervical mucus: add 0.15ml of cervical mucus to three clean test tubes, add 0.01ml, 0.02ml, and 0.03ml of liquid medicine respectively, shake well or blow several times with a straw. Observe with the naked eye to see the degree of stringiness, dry the smear, and see fern-shaped crystals under a light microscope.

c. The effect of the composition of the present invention on the mixed liquid of semen and cervical mucus: get 0.1ml of semen and 0.1ml of cervical mucus and mix well, add 0.02ml of the pharmaceutical composition emulsion of the present invention and mix well, get a drop of the mixed solution on a glass slide, add Observe under the microscope after covering the glass, and record the sperm motility, vitality, density, etc.

d. Glass slide penetration test: take 20 μl of the emulsion of the pharmaceutical composition of the present invention and cervical mucus mixture in the three test tubes described in 3 respectively on the slide, take an equal amount of semen and put it on the same slide at a distance of 4mm, press the cover The slides were observed with a phase-contrast microscope.

e. Capillary climbing test: Take the first tube of semen in b in a special plastic semen storage tank, fill the capillary with cervical mucus, seal the top with plasticine, insert the other end into the semen storage tank, and immerse the semen at a depth of about 2mm. The cervical mucus column is more than 5cm long. Put it into a 37°C environment, time it, and place the capillary flat on an inverted phase-contrast microscope at 30 minutes, 60 minutes, and 120 minutes respectively for observation. The semen test method of the 2nd tube and the 3rd tube is the same as above, and the semen that does not add the pharmaceutical composition emulsion of the present invention is used as a parallel control simultaneously.

(4) Experimental results:

a. the composition of the present invention is to the effect of seminal fluid, as shown in table 3, the motility and viability of sperm in each group's seminal fluid are all reduced to 0, and density is reduced to 20-40 after pharmaceutical composition emulsion medication of the present invention 1 hour; After taking 3ml of the inventive pharmaceutical composition emulsion, the motility and motility rate of the sperm are both 0 after 20s, and the density is 20×10 ⁹ -40×10 ⁹ L ^-1 . When the concentration is 0.024%, sperm can be inactivated instantly, and when the concentration is 0.008%, all sperm can be inactivated within 1 hour, and the spermicidal effect is better than that used alone. It can be seen that the composition of the present invention has a good spermicidal effect.

Table 3 The results of the effect of medicinal liquid on semen time blank control Composition of the present invention/0.02ml Composition of the present invention/0.02ml Composition of the present invention/0.03ml vitality Survival rate% Density×10 ⁹ L ^-1 vitality Survival rate% Density×10 ⁹ L ^-1 vitality Survival rate% Density×10 ⁹ L ^-1 vitality Survival rate% Density×10 ⁹ L ^-1 20S IV 70 >50 IV 70 >50 II 40 >50 0 0 20-40 1min IV 70 >50 III 60 >50 I 20 >50 0 0 20-40 15min IV 70 >50 II 40 >50 0 0 20-40 0 0 20-40 30min IV 70 >50 I 20 >50 0 0 20-40 0 0 20-40 60min III 60 >50 0 0 20-40 0 0 20-40 0 0 20-40

b. The effect of the composition of the present invention on cervical mucus: immediately after adding the emulsion of the composition of the present invention, white flocs appear, and milky white solidified masses can be seen when blowing with a straw. degrees disappear. After drying the smear of the mixture, no fern-shaped crystals can be seen in the coagulated mass under the light microscope, and fern-shaped crystals can be seen in the eluate, indicating that the composition of the present invention can change the physical and chemical properties of cervical mucus.

c. The effect of the composition of the present invention on the mixed liquid of semen and cervical mucus: add 0.02 ml of the emulsion of the pharmaceutical composition of the present invention and mix well, then observe under the microscope. It can be seen that all the spermatozoa are immobilized. Density decreases and filaments increase.

d. Result of slide penetration test: After adding the emulsion of the pharmaceutical composition of the present invention, none of them made the sperm penetrate. Under the microscope, it can be seen that there are finger-like protrusions at the contact part of the semen, but the sperm at each protruding interface is also braked. And as the emulsion flows in, the motile sperm that come into contact with the emulsion are also braked.

e. The results of the capillary climbing test, the results are shown in Table 4.

Table 4 Capillary climb test results time/min Composition of the present invention/0.02ml Composition of the present invention/0.02ml Composition of the present invention/0.03ml normal control group 30 no penetration no penetration no penetration A large number of motile sperm can be seen at 1.5cm 60 Several motile spermatozoa were seen at 0.5cm no penetration no penetration A large number of motile sperm can be seen at 3cm 120 Several motile spermatozoa were seen at 0.5cm Several motile spermatozoa were seen at 0.5cm no penetration A large number of motile sperm can be seen at 5cm

Conclusion: The combination of zedoary oil and hyodeoxycholic acid not only has a strong spermicidal effect, but also can change the physical and chemical properties of semen and cervical mucus to prevent conception.

2. Anti-vaginal infection experiment

(1) Test drug:

Pharmaceutical composition of the present invention: A. the emulsion of turmeric oil 4%, hyodeoxycholic acid 2%, Tween-80 2% and 0.9% sodium chloride,

B. Emulsion of turmeric oil 2%, hyodeoxycholic acid 1%, Tween-80 2% and 0.9% sodium chloride;

Povidone-iodine ointment (batch number 06060305, produced by Guangdong Zhongsheng Pharmaceutical Co., Ltd.): use physiological saline to prepare a solution containing 10% povidone-iodine.

(2) Experimental materials:

New Zealand white rabbits were purchased from the Experimental Animal Center of Guangzhou University of Traditional Chinese Medicine.

Staphylococcus aureus, Escherichia coli, B streptococcus, Candida albicans. Standard strains were provided by China National Institute for the Control of Pharmaceutical and Biological Products.

(3) Experimental method:

a. Antibacterial test in vitro

Get the pharmaceutical composition A of the present invention and do 2 times ratio dilution (petri dish method), multi-point quantitative inoculation adds standard bacterial liquid, cultivates 7d in 37 ℃ of incubators, simultaneously controls and bacterial classification control same cultivation 7d, observes the bacteriostasis of each concentration Effect.

b. The therapeutic effect of experimental rabbit vaginitis

Take 36 female rabbits with a body weight of about 3kg, 4 of which are used as the normal control group, and the remaining 32 are respectively given the culture solution of Streptococcus beta, Staphylococcus aureus, and Candida albicans mixed at a ratio of 1:1:1, and the ratio of 0.4 ml/only, inject the mixed bacterial solution into the vagina of rabbits. After injecting the bacterial solution, move freely, and repeat the infection again in the same way after 8 hours. After 3 days, the vaginal secretions were taken for microscopic examination and culture, and those whose vaginal secretions were all positive for the three bacterial tests were qualified as test animals. Qualified animals with positive test results were randomly divided into 4 groups, model group, original concentration zedoary oil, hyodeoxycholic acid composition group, 50% concentration zedoary oil, hyodeoxycholic acid composition group and 10% povidone-iodine ointment control group Group. Each rat was administered once a day at 8:00Am and 8:00Pm for 10 consecutive days, and the control group was given equal volume of normal saline. The administration method is that the animal is placed in the supine position, and the vagina is rinsed with a latex tube. The volume of administration is 2mL per animal. After each administration, the animal moves freely immediately and allows the liquid to flow out without cleaning and washing. 2 hours after the last administration, all animals were killed, and the vaginas of each rabbit were dissected, cut open along the longitudinal axis of the vaginas, and observed with the naked eye for redness, swelling, edema, and abscesses in the mucous membranes of the vaginas and cervix, and then fixed with 10% formaldehyde for histopathological examination .

(4) Results

a. Antibacterial test in vitro: See Table 1 and Table 2 for specific results.

Table 1. In vitro antibacterial test results of the pharmaceutical composition of the present invention Dilution times 1:2 1:4 1:8 1:16 1:32 1:64 Strain control E. J E. J E. J E. J E. J E. J Staphylococcus aureus - - - - - - - - + + + + + + Escherichia coli - - - - - - ± ± + + + + + + B Streptococcus - - - - - - ± ± + + + + + + Candida albicans - - - - - - - - + + + + + +

Note: E indicates the composition of turmeric oil and hyodeoxycholic acid, J indicates povidone-iodine ointment; - indicates no growth of corresponding bacteria, + indicates growth of corresponding bacteria, ± indicates weak growth of corresponding bacteria.

The results showed that zedoary oil, hyodeoxycholic acid composition and povidone-iodine ointment could all inhibit the growth of various bacteria.

Table 2 The MIC (g/ml) of the composition of the present invention, zedoary oil and hyodeoxycholic acid to various pathogenic bacteria bacteria Curcuma Oil hyodeoxycholic acid Composition of the invention Staphylococcus aureus 0.005 >0.01 0.001 Beta-hemolytic Streptococcus 0.005 0.01 0.01 Escherichia coli 0.01 0.05 0.01 Pseudomonas aeruginosa >0.05 0.01 >0.01 Candida albicans 0.005 0.05 0.005 Gardnerella vaginalis 0.005 0.01 0.001 Neisseria gonorrhoeae 0.001 0.01 0.001 Average MIC >0.011 >0.021 >0.005

Note: MIC refers to the minimum drug concentration that completely inhibits the growth of the test bacteria.

The results show that the MIC of the composition of the present invention to various vaginitis pathogenic bacteria is lower than that of zedoary oil and hyodeoxycholic acid, and some of the reductions are as high as 10 times. It can be seen that the composition of the present invention has a strong antibacterial effect.

b. Experimental Rabbit Vaginitis Experiment

The inflammatory infiltration of the vaginal wall, mucosal hyperplasia and interstitial edema were used as the main histological indicators, and the semi-quantitative grading was carried out, and the model control group was used as the reference system (grade index M=0), and the significance of the difference between groups was carried out by the grade index method The test results are shown in Table 2.

            Table 2 Effects on the Pathomorphology of Infectious Vaginitis

table 2-1 group N (only) Inflammatory infiltration #(Only) +++(only) ++(only) +(only) m T normal group 4 not obvious model group 8 5 3 0 0 0 Composition A group of the present invention 8 0 0 3 5 5.83 2.71 Composition B group of the present invention 8 0 0 5 3 4.94 2.28 povidone iodine group 8 0 0 4 4 5.43 2.38

Table 2-2 group N (only) mucosal hyperplasia #(Only) +++(only) ++(only) +(only) m T normal group 4 not obvious model group 8 6 2 0 0 0 Composition A group of the present invention 8 0 0 4 4 5.43 2.38 Composition B group of the present invention 8 0 0 6 2 4.47 2.43 povidone iodine group 8 0 0 3 5 5.83 2.71

Table 2-3 group N (only) edema #(Only) +++(only) ++(only) +(only) m T normal group 4 not obvious model group 8 4 4 0 0 0 Composition A group of the present invention 8 0 0 3 5 5.83 2.71 Composition B group of the present invention 8 0 0 4 4 5.43 2.38 povidone iodine group 8 0 0 4 4 5.43 2.38

Note: M is grade index, a positive value indicates that the morphology indicator is better than the model group, and the larger the value, the better the morphology. T is the value of the t test with the model group, T _0.05 =1.98. + means occasionally a small amount of white blood cell infiltration, mucous membrane redness, swelling and congestion, and slight edema; ++ means a small amount of inflammatory leachate, slight hyperplasia of mucosa, and edema; +++ means obvious exudation of inflammatory leachate, obvious mucosal hyperplasia, and obvious edema; # Indicates severe exudation of inflammatory leachate, severe mucosal hyperplasia, and severe edema.

The results are shown in Table 2, the composition of the present invention can have obvious protective effect on the vaginal mucosal lesion of rabbit vaginitis caused by mixed bacterial infection, can effectively treat vaginitis, and its curative effect is equivalent to that of povidone-iodine.

In summary, the composition of the invention can kill various vaginal pathogenic bacteria and treat experimental vaginitis caused by mixed infection of various bacteria.

Detailed ways

Example 1 (lotion)

1. The pharmaceutical composition of the present invention: turmeric oil 0.5g, hyodeoxycholic acid 20g

2. Preparation of lotion: take 70ml of deionized water, add hyodeoxycholic acid, add about 20ml of 10% sodium hydroxide solution dropwise to dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid aqueous solution; take turmeric oil and 2g Mix with Tween 80, add to hyodeoxycholic acid aqueous solution, adjust the weight to 100g with deionized water, mix well, and obtain. For external use, take 1-10ml of this product each time, add 1000ml of water to dilute, and then wipe clean or take a hip bath.

Example 2 (lotion)

1. The pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of lotion: take 70ml of deionized water, add hyodeoxycholic acid, add about 2ml of 10% sodium hydroxide solution dropwise to dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid aqueous solution; take turmeric oil and 8g Mix with Tween 80, add to hyodeoxycholic acid aqueous solution, adjust the weight to 100g with deionized water, mix well, and obtain. For external use, take 1-10ml of this product each time, add 1000ml of water to dilute, and then wipe clean or take a hip bath.

Example 3 (ointment)

1. The pharmaceutical composition of the present invention: turmeric oil 20g, hyodeoxycholic acid 0.5g.

2. Preparation of ointment: Take 20ml of deionized water, add hyodeoxycholic acid, add about 0.5ml of 10% sodium hydroxide solution dropwise to dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid aqueous solution, add 1g of carbopol Glycerin, 4g glycerin, mix well to get the water phase; take zedoary oil and mix it with 20g Tween 80 to get the oil phase, mix the water phase and the oil phase, emulsify with a high-speed homogenizer, adjust the weight to 100g with deionized water, add Hyodeoxycholic acid aqueous solution, mixing, that is. For external use, take 1-2g of this product each time and apply it to the affected area.

Example 4 (ointment)

1. The pharmaceutical composition of the present invention: turmeric oil 10g, hyodeoxycholic acid 2g.

2. Preparation of ointment: Take 20ml of deionized water, add hyodeoxycholic acid, add about 2ml of 10% sodium hydroxide solution dropwise to dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid aqueous solution, add 1g of carbomer , 4g glycerin, mix well to get the water phase; take zedoary oil and mix it with 10g Tween 80 to get the oil phase, mix the water phase and the oil phase, emulsify with a high-speed homogenizer, adjust the weight to 100g with deionized water, mix well , that is. For external use, take 1-2g of this product each time and apply it to the affected area.

Example 5 (Gel)

1. The pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of the gel: Take 80ml of deionized water, add hyodeoxycholic acid, drop about 2ml of 10% sodium hydroxide solution to dissolve the hyodeoxycholic acid, filter to obtain a hyodeoxycholic acid aqueous solution, add 40g of Poloxamer 407 , 3g methyl cellulose, 4g glycerin, mix well to get the water phase; take zedoary oil and mix it with 8g Tween 80 to get the oil phase, mix the water phase and the oil phase, emulsify with a high-speed homogenizer, adjust with deionized water The weight reaches 100g, mix well, and you get it. For external use, take 1-2g of this product each time and apply it to the affected area.

Example 6 (Aerosol)

1. The pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of foam agent: Take 20ml of ethanol, add hyodeoxycholic acid, dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid ethanol solution, add 5g of benzyl alcohol, 4g of turmeric oil, 808g of Tween, 4g of glycerin, Triethanolamine 1g, stearic acid 2g and Pingjiao 10g, mix well, emulsify with a high-speed homogenizer, add distilled water to adjust to 100g; pour it into a sealed container together with dimethyl ether, and obtain. For external use, take 1-2g of this product each time and spray it on the affected area.

Example 7 (foaming agent)

1. The pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of aerosol: Take 20ml of ethanol, add hyodeoxycholic acid, dissolve hyodeoxycholic acid, filter to obtain hyodeoxycholic acid ethanol solution, add 5g of benzyl alcohol, 4g of zedoary oil, Tween 80 8g, glycerin 4g, 1g of triethanolamine, 2g of stearic acid, and 10g of Oxygenate, mix well, emulsify with a high-speed homogenizer, add distilled water to adjust to 100g; pour into a sealed container to obtain. For external use, take 1-2g of this product each time and spray it on the affected area.

Example 8 (suppository)

1, pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of suppositories: take 80 g of cocoa butter, add hyodeoxycholic acid and zedoary oil to make 100 suppositories in total. For external use, take 1 capsule of this product each time and place it in the female vagina.

Example 9 (Effervescent Tablets)

1. The pharmaceutical composition of the present invention: turmeric oil 4g, hyodeoxycholic acid 2g.

2. Preparation of effervescent tablets: Take 20g of cyclodextrin, add zedoary oil, stir to make cyclodextrin include zedoary oil, add 2g of hyodeoxycholic acid, 7.5g of boric acid, 22.5g of tartaric acid, 30g of sodium bicarbonate, add starch Adjust the weight to 100g, press into tablets, 1-2g per tablet, and you get it. For external use, take 1 capsule of this product each time and place it in the female vagina.

Claims (10)

1. A pharmaceutical composition for killing sperm and treating vaginitis, the composition is composed of active ingredients and medically acceptable auxiliary materials, and is characterized in that it contains the following active ingredients in weight percent: zedoary oil 0.5-20%, porcine deoxygenation Cholic acid 0.5-20%. 2. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 1, which is characterized in that it contains the following active ingredients in weight percentage: zedoary oil 4%, hyodeoxycholic acid 2%. 3. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 1 or 2, characterized in that the composition is lotion, ointment, gel, foam, suppository, aerosol or vaginal effervescent tablet. 4. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the auxiliary materials of the lotion are Tween-80 and water. 5. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the auxiliary materials of the ointment are carbomer, glycerin and emulsifier Tween-80. 6. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the excipients of the gel are Poloxamer 407, methylcellulose, glycerin and Tween-80. 7. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the auxiliary material of the suppository is cocoa butter. 8. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the auxiliary materials of the aerosol are Tween-80, glycerin, benzyl alcohol, triethanolamine, stearic acid, Pingpingjiao and dimethyl ether. 9. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the excipients of the vaginal effervescent tablet are cyclodextrin, boric acid, tartaric acid and sodium bicarbonate. 10. The pharmaceutical composition for killing sperm and treating vaginitis according to claim 3, characterized in that the excipients of the foaming agent are Tween-80, glycerin, benzyl alcohol, triethanolamine, stearic acid and Pingjiao O.