CN100372522C - skin care products - Google Patents

Abstract

The invention provides a skin care product, the main components of the skin care product and the content of the components by weight ratio are: recombinant human epidermal growth factor 5×10 ^-7 to 5×10 ^-4 %, recombinant human alkali Sexual fibroblast growth factor 3×10 ^-7 ～7×10 ^-4 %, zinc ion 1.3×10 ^-5 ～6.8×10 ^-4 %, calcium ion 0.01～1.5%, magnesium ion 0.05～5.0%, N- 0.01-2.0% of 2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, 0.01-10.0% of L-glutamine, and the balance of excipients; the skin care products provided by the invention can rapidly improve skin cell Survival microenvironment, the skin becomes ruddy; fade trauma spots, sunburn, freckles, chloasma; fade depigmentation spots; eliminate wrinkles; eliminate dark circles and bags under the eyes; smooth scars; relieve hyperkeratosis of the skin More than a month, it can make the skin of the whole body gradually change, become fine and smooth, keep the epithelial tissue healthy, enhance the disease resistance, no allergies and other adverse reactions during use, continue to be effective for long-term use, and will not rebound after stopping use.

Description

skin care products

technical field

The invention relates to a cosmetic, in particular to a skin care product for nourishing the skin.

Background technique:

The nourishing care of the skin has always been the focus of attention of people, especially women, and people have been looking for skin care products that can whiten and nourish the skin and have no side effects. The manufacturing technology of skin care products started relatively late in my country, probably in the 1980s, and has a long history of research and development abroad. However, due to the limitations of scientific development, most of them are still at the level of experience, and the actual efficacy is limited or not significant. Skin care products composed of chemical raw materials are not perfect. It shows that the prescription is unscientific or contains substances harmful to the human body, such as containing a large amount of phosphorus. Phosphorus will hinder the body's absorption of calcium, an important substance in the human body. For example, world-renowned brands and some new domestic brand skin care products contain acrylamide. This raw material has a highly toxic effect on the nervous system and is a carcinogen. It is recognized; there is also the widespread use of mercury-containing compounds in skin care products as whitening agents and preservatives, etc., which makes people worry about this type of skin care products on the market today, and their efficacy is not satisfactory. Products formulated with plant extracts, because the extraction method is simple, the components in the crude extract are complex and uncertain, and the efficacy is naturally uncertain. Skin care products formulated with animal embryo tissue ingredients. Contains hormones, although it has significant effects, but its hidden dangers are also obvious, and it will rebound after stopping use.

Chinese patent 96119195.3 provides a cosmetic additive to nourish the skin. This solution uses a variety of amino acids with high purity to nourish the skin, but amino acids alone are obviously not enough for nourishing the skin: it cannot moderately activate the proliferation of skin stem cells and improve the replacement of old and new cells ; Can not effectively regulate the enzyme reaction of skin cells; For protecting skin cells from oxidation and keeping skin moist, the effect of filling skin tissue fluid is insufficient.

Contents of the invention

The present invention aims to solve the above technical problems and provide a safe and effective skin nourishing and skin care product.

The present invention achieves the above object through the following technical solutions.

The invention provides a skin care product, the components of which include recombinant human epidermal growth factor, recombinant human basic fibroblast growth factor, zinc ion, calcium ion, magnesium ion, N-2-hydroxyethylpiperazine-N '-2-ethanesulfonic acid, L-glutamine and excipients.

Wherein the content of described composition by weight ratio is:

Recombinant human epidermal growth factor 5x10 ^-7 ～5x10 ^-4 %

Recombinant human basic fibroblast growth factor 3x10 ^-7 ～7x10 ^-4 %

Zinc ion 1.3x10 ^-5 ～6.8x10 ^-4 ％

Calcium ions 0.01～1.5%

Magnesium ions 0.05～5.0%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 0.01～2.0%

L-Glutamine 0.01～10.0%

Excipients Surplus.

The zinc ion source is selected from zinc sulfate, zinc citrate, zinc carbonate, zinc chloride and zinc glucose. The calcium ion source is selected from calcium chloride, calcium sulfate, calcium gluconate, calcium citrate, calcium fruitate, calcium malate, calcium carbonate and vitamin C calcium. The magnesium ion source is selected from magnesium sulfate, magnesium chloride magnesium hydrogen phosphate, magnesium dihydrogen phosphate and magnesium carbonate.

The auxiliary materials are selected from thickeners, preservatives, cooling agents, buffers, pH regulators, colorants, surfactants, astringents, stabilizers, solvents and spices.

The skin care product provided by the present invention also includes the following ingredients: 0.01-10.0% of L-glutamine, 0.03-7.0% of isoleucine, 0.05-6.0% of proline, 0.03-8.5% of asparagine, 0.01-2.0% of potassium ions, 0.01-4.0% of sodium ions, 0.01-2.0% of glucose and the balance of auxiliary materials.

Skin care product provided by the present invention, its component and percentage by weight are preferably:

Recombinant human epidermal growth factor 5x10 ^-7 ～5x10 ^-4 %

Recombinant human basic fibroblast growth factor 3x10 ^-7 ～7x10 ^-4 %

Zinc ion 1.3x10 ^-5 ～6.8x10 ^-4 ％

Calcium ions 0.01～1.5%

Magnesium ions 0.05～5.0%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 0.01～2.0%

L-Glutamine 0.01～10.0%

L-Isoleucine 0.03～7.0%

L-proline 0.05～6.0%

L-Asparagine 0.03～8.5%

L-cysteine 0.01～6.5%

L-Arginine 0.03～6.0%

L-Hydroxyproline 0.02～9.0%

L-histidine 0.01～5.5%

L-Leucine 0.01～7.5%

L-Lysine 0.01～8.5%

L-Methionine 0.03～5.5%

L-Phenylalanine 0.03～8.5%

L-serine 0.01～6.7%

L-Threonine 0.02～7.6%

L-Tryptophan 0.01～8.4%

L-Tyrosine 0.03～9.1%

L-Valine 0.01～7.1%

L-glutamic acid 0.03～6.4%

L-Aspartic Acid 0.02～5.8%

L-alanine 0.01～7.2%

Glycine 0.01～8.2%

KCl 0.01～2

NaCl 0.01～1.0%

NaHCO ₃ 0.01～1.2%

Na ₂ HPO ₄ 0.01～1.0%

Sodium pyruvate 0.01～0.8%

Glucose 0.01～2.0%

Vitamin 1x10 ^-4 ～3x10 ^-3 %

Excipients Surplus.

After the skin care product provided by the present invention is used on the surface of human skin, it has the following effects:

1) Rapidly improve the living microenvironment of skin cells, and the tissue cells can be seen to become clear under the microscope;

2) Facial skin becomes ruddy;

3) Fades trauma spots, sunburn, freckles, and chloasma caused by endocrine disorders;

4) Fades depigmentation spots;

5) Eliminate wrinkles;

6) Eliminate dark circles and eye bags;

7) smooth scars;

8) Improve hyperkeratosis of the skin. Continuous use for more than three months can make the skin of the whole body gradually change, become fine and smooth, keep the epithelial tissue healthy and enhance the disease resistance;

9) There are no allergies and other adverse reactions during use, and it will continue to be effective after long-term use, and there will be no rebound after stopping use.

The skin care product provided by the present invention has the following advantages with respect to the prior art, especially Chinese patent No. 96119195.3:

1. When skin cells perform normal physiological functions, they need the participation of many messenger molecules. These molecules can be proteins, polypeptides, small organic molecules and inorganic ions. Epidermal growth factor and basic fibroblast growth factor are involved in the regulation of cell proliferation cycle, and they can effectively initiate cell proliferation. The skin care product provided by the present invention contains these two ingredients, which solves the technical problem of moderately activating the proliferation of skin stem cells, accelerates the replacement of old and new skin cells, and presents a youthful state, with remarkable technical effects.

2. The Zn ²⁺ and Ca ²⁺ contained in the skin care products provided by the present invention are very important substances for skin cells and are activators of various enzyme reactions in the body.

3. The Mg ²⁺ contained in the skin care products provided by the present invention participates in the biological oxidation process of sugar in cells, and provides sufficient ATP energy supply for skin cells to carry out normal life activities.

4. To protect skin cells from oxidation, the damage of ultraviolet radiation to the structure of genetic material DNA, protein and polypeptide in cells is also an important issue. Reduced glutathione is a good antioxidant for cells in the body. The skin care product provided by the present invention adds natural amino acid L-glutamine, which is one of the important raw materials for synthesizing reduced glutathione in vivo.

5. Keeping the skin moist, filling the skin tissue fluid, and maintaining the neutral pH microenvironment of the skin are the prerequisites for the skin cells to perform the above important physiological functions. The skin care product provided by the present invention uses N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid for the first time, which has strong moisturizing and pH buffering effects.

Based on the above points, the skin care product provided by the present invention is a comprehensive and complete nutritional system, from the protection of DNA in the nucleus of skin cells, to the regulation of cell proliferation at the gene level, to the activation of cell metabolism, to the strengthening of cell energy supply, It has given good maintenance to the stability of skin tissue structure.

Detailed ways:

The following examples and experimental examples are intended to better explain the technical solutions provided by the present invention, rather than to limit the present invention. All modifications and variations based on the idea of the present invention belong to the protection scope of the present invention.

Example 1.

Recombinant Human Epidermal Growth Factor 5x10 ^-7 %

Recombinant Human Basic Fibroblast Growth Factor 7x10 ^-4 %

ZnSO ₄ ^6.8x10-4 %

_CaCl2 1.5%

_MgSO4 5.0%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 2.0%

L-Glutamine 10.0%

Spices 2.0%

Hydagen_CMF Chitin 9.5%

Deionized water balance

A proper amount of emulsifier is mixed with dipropylene glycol and perfume in advance, and the above raw materials are mixed to obtain a finished skin care product.

Example 2.

Recombinant Human Epidermal Growth Factor 5x10 ^-4 %

Recombinant Human Basic Fibroblast Growth Factor 3x10 ^-7 %

Zinc gluconate 1.3x10 ^-5 %

Calcium citrate 0.01%

Magnesium Chloride 0.05%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 0.01%

L-Glutamine 0.01%

L-Glutamine 7.0%

Isoleucine 7.0%

Proline 6.0%

Asparagine 8.5%

Potassium ions 2.0%

Sodium ion 4.0%

Glucose 2.0%

Spices 0.2%

Methyl Hydroxypropyl Cellulose 0.3%

Chitin 0.3%

Deionized water balance.

A proper amount of emulsifier is mixed with dipropylene glycol and perfume in advance, and the above raw materials are mixed to obtain a finished skin care product.

Example 3.

Recombinant Human Epidermal Growth Factor 2x10 ^-5 %

Recombinant Human Basic Fibroblast Growth Factor 5x10 ^-5 %

Zinc citrate 3.2x10 ^-4 %

Calcium Carbonate 0.7%

Magnesium Carbonate 2.0%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 1.0%

L-Glutamine 5.0%

L-Isoleucine 7.0%

L-Proline 6.0%

L-Asparagine 8.5%

L-cysteine 6.5%

L-Arginine 6.0%

L-Hydroxyproline 9.0%

L-Histidine 5.5%

L-Leucine 7.5%

L-Lysine 8.5%

L-Methionine 0.03%

L-Phenylalanine 0.03%

L-serine 0.01%

L-Threonine 0.02%

L-Tryptophan 0.01%

L-Tyrosine 0.03%

L-Valine 0.01%

L-glutamic acid 0.03%

L-Aspartic Acid 0.02%

L-alanine 0.01%

Glycine 0.01%

KCl 0.01%

NaCl 0.01%

NaHCO ₃ 0.01%

Na ₂ HPO ₄ 0.01%

Sodium pyruvate 0.01%

Glucose 0.01%

Carbomer 0.5%

Glycerol 9%

Spices 0.5%

Propylparaben 0.08%

Deionized water balance.

Dissolve carbomer in an appropriate amount of deionized water, add glycerin to obtain solution A, make solution B from other raw materials, slowly add solution B to solution A and stir, then add propylparaben, and stir rapidly, Makes into a clear gel.

Example 4.

Recombinant Human Epidermal Growth Factor 1x10 ^-5 %

Recombinant Human Basic Fibroblast Growth Factor 1x10 ^-5 %

Zinc carbonate 1.3x10 ^-5 %

Calcium Fruit Acid 0.01%

Magnesium dihydrogen phosphate 0.05%

N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid 0.01%

L-Glutamine 0.01%

L-Isoleucine 0.03%

L-Proline 0.05%

L-Asparagine 0.03%

L-cysteine 0.01%

L-Arginine 0.03%

L-Hydroxyproline 0.02%

L-Histidine 0.01%

L-Leucine 0.01%

L-Lysine 0.01%

L-Methionine 5.5%

L-Phenylalanine 8.5%

L-serine 6.7%

L-Threonine 7.6%

L-Tryptophan 8.4%

L-Tyrosine 9.1%

L-Valine 7.1%

L-glutamic acid 6.4%

L-Aspartic Acid 5.8%

L-alanine 7.2%

Glycine 8.2%

KCl 2.0%

NaCl 1.0%

NaHCO ₃ 1.2%

Na ₂ HPO ₄ 1.0%

Sodium pyruvate 0.8%

Glucose 2.0%

Carbomer 0.5%

Spices 0.5%

Propylparaben 0.08%

Vitamin C ^1x10-4 %

Vitamin E ^2x10-3 %

Deionized water balance.

Dissolve carbomer in an appropriate amount of deionized water, add glycerin to obtain solution A, make solution B from other raw materials, slowly add solution B to solution A and stir, then add propylparaben, and stir rapidly, Makes into a clear gel.

Experiment 1: Experiment of promoting epithelial stem cell proliferation of skin care products provided by the present invention

Method: Take 3 pieces of ^adult abdominal surface and base skin with a size of 1×1 mm, wash, mix and cut them into two petri dishes, use the skin care product nutrient solution provided by the present invention for No. They were cultured separately with common laboratory cell culture medium. After 3 days, the suspended tissue mass was discarded, leaving the adherent cells. After digestion, the epithelial stem cells were screened by 96-well plate clone screening method, and the growth of the clone groups in the two dishes was observed daily. Two weeks later, immunocytochemical staining of epithelial stem cell markers CK19 and PCNA was performed to identify the size of epithelial stem cells and clonal populations.

Experimental results: The epithelial stem cells grown on the No. 1 96-well culture plate spread out in a large clonal group, and the number of cells and the adherent area were significantly larger than those grown on the No. 2 96-well culture plate. There was a significant difference between the two.

Experiment 2: Observation experiment of moisturizing effect of skin care products provided by the present invention

Methods: 35-73 years old women were selected, 5 cases each with dry skin, normal skin and oily skin. Left and right hand ring fingers are observation objects, the left is a control group, the right is a moisturizing group, the moisturizing group uses the skin care products provided by the present invention, and the control group uses pure double distilled water. Under a microscope, observe the smoothness, glossiness and depth of skin grooves on the skin surface.

result:

dry skin normal skin oily skin Moisturizing group (5 cases each) control group (5 cases each) Markedly effective in 3 cases and better in 2 cases- Significantly effective in 5 cases- Significantly effective in 5 cases-

Conclusion: The moisturizing effect of the moisturizing group was significantly better than that of the control group, and none of them became worse.

Experiment 3: overall evaluation of skin care products provided by the present invention

Experimental method: Select 21 females and 19 males around 40 years old, and randomly divide them into four groups of 10 each, and each group includes males and females. Wherein the first group uses the skin care product A prepared by the amino acid component in the skin care product formula provided by the present invention; the second group uses the skin care product B prepared by the cell growth factor used in the skin care product A and the skin care product provided by the present invention ; The third group uses skin care product A and Zn ²⁺ , Ca ²⁺ , Mg ²⁺ formulated skin care product C; the fourth group uses the skin care product provided by the present invention. Each group of formulations was used separately for two months. Before use, one month and two months after use, use the Japanese NAUMP professional skin detection and analysis system to test and analyze the ruddyness, elasticity, pigmentation, and wrinkles of the skin on the same part of the face. The results are shown in the table below. Conclusion: the skin care product provided by the invention can significantly improve the nutritional efficacy of skin cells.

Ruddy degree elasticity faded spots wrinkle removal Ability to repair damage The first group, the second group, the third group, the fourth group +++++++++ -++++++++ -++++++ -++++++++ +++++++++

Efficacy indication: no effect -, average +, more obvious ++, very obvious +++.

Claims (9)

1. skin care item is characterized in that comprising recombinant human epidermal growth factor, recombination human basic fibroblast growth factor, zinc ion, calcium ion, magnesium ion, N-2-hydroxyethyl piperazine-N '-2-ethanesulfonic acid, L-glutaminate and adjuvant in the component of described skin care item.

2. skin care item as claimed in claim 1, wherein the content in the described composition of weight ratio is:

Recombinant human epidermal growth factor 5x10 ^-7～5x10 ^-4%

Recombination human basic fibroblast growth factor 3x10 ^-7～7x10 ^-4%

Zinc ion 1.3x10 ^-5～6.8x10 ^-4%

Calcium ion 0.01～1.5%

Magnesium ion 0.05～5.0%

N-2-hydroxyethyl piperazine-N '-2-ethanesulfonic acid 0.01～2.0%

L-glutaminate 0.01～10.0%

The adjuvant surplus.

3. skin care item as claimed in claim 1, wherein said zinc ion source is selected from zinc sulfate, zinc citrate, zinc carbonate, zinc chloride and zinc gluconate.

4. the wherein said calcium ion source of skin care item as claimed in claim 1 is selected from calcium chloride, calcium sulfate, calcium gluconate, calcium citrate, calcium citrate malate, calcium malate, calcium carbonate and calcium ascorbate.

5. skin care item as claimed in claim 1, wherein said magnesium ion source is selected from magnesium sulfate, magnesium chloride, magnesium hydrogen phosphate, Magnesium biphosphate and magnesium carbonate.

6. skin care item as claimed in claim 1, wherein said adjuvant are selected from thickening agent, antiseptic, freshener, buffer agent, pH regulator agent, coloring agent, dispersant, surfactant, astringent, stabilizing agent, solvent and spice.

7. skin care item as claimed in claim 1, wherein said component also comprises following composition: isoleucine, proline, agedoite, potassium ion, sodium ion and glucose.

8. skin care item as claimed in claim 7, wherein the content in the described composition of weight ratio is:

Recombinant human epidermal growth factor 5x10 ^-7～5x10 ^-4%

Recombination human basic fibroblast growth factor 3x10 ^-7～7x10 ^-4%

Zinc ion 1.3x10 ^-5～6.8x10 ^-4%

Calcium ion 0.01～1.5%

Magnesium ion 0.05～5.0%

N-2-hydroxyethyl piperazine-N '-2-ethanesulfonic acid 0.01～2.0%

L-glutamy 0.01～10.0%

Isoleucine 0.03～7.0%

Proline 0.05～6.0%

Agedoite 0.03～8.5%

Potassium ion 0.01～2.0%

Sodium ion 0.01～4.0%

Glucose 0.01～2.0%

The adjuvant surplus.

9. as the arbitrary described skin care item of claim 1～8, its component and percentage by weight are:

Recombinant human epidermal growth factor 5x10 ^-7～5x10 ^-4%

Recombination human basic fibroblast growth factor 3x10 ^-7～7x10 ^-4%

Zinc ion 1.3x10 ^-5～6.8x10 ^-4%

Calcium ion 0.01～1.5%

Magnesium ion 0.05～5.0%

N-2-hydroxyethyl piperazine-N '-2-ethanesulfonic acid 0.01～2.0%

L-glutaminate 0.01～10.0%

L-isoleucine 0.03～7.0%

L-proline 0.05～6.0%

Altheine 0.03～8.5%

L-cysteine 0.01～6.5%

L-arginine 0.03～6.0%

L-hydroxyproline 0.02～9.0%

L-histidine 0.01～5.5%

L-leucine 0.01～7.5%

L-lysine 0.01～8.5%

L-methionine 0.03～5.5%

L-phenylalanine 0.03～8.5%

L-serine 0.01～6.7%

L-threonine 0.02～7.6%

L-tryptophan 0.01～8.4%

L-tyrosine 0.03～9.1%

L-valine 0.01～7.1%

L-glutamic acid 0.03～6.4%

L-aspartic acid 0.02～5.8%

L-alanine 0.01～7.2%

Glycine 0.01～8.2%

KCl 0.01～2.0％

NaCl 0.01～1.0％

NaHCO ₃ 0.01～1.2％

Na ₂HPO ₄ 0.01～1.0％

Sodium Pyruvate 0.01～0.8%

Glucose 0.01～2.0%

Vitamin 1x10 ^-4～3x10 ^-3%

The adjuvant surplus.