CN100376665C - Cell culturation apparatus - Google Patents
Cell culturation apparatus Download PDFInfo
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- CN100376665C CN100376665C CNB2005101344892A CN200510134489A CN100376665C CN 100376665 C CN100376665 C CN 100376665C CN B2005101344892 A CNB2005101344892 A CN B2005101344892A CN 200510134489 A CN200510134489 A CN 200510134489A CN 100376665 C CN100376665 C CN 100376665C
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Abstract
The present invention discloses a cell culture device which comprises a cell culture tank, a nutrient solution circulating device, a cell supplying device and a cell collecting device, wherein one end of the cell culture tank is provided with an inlet for seed cells, an inlet for nutrient solution and an adding port for matrix cells, and the other end is provided with an outlet for cultured cells, an outlet for metabolized waste solution and a discharge port for the matrix cells; at least two supplying tubes for the nutrient solution, one recovery tube for the metabolized waste solution and two cell culture tubes are arranged in the cell cultivation tank of which other places are filled with the matrix cells. The nutrient solution circulating device is respectively communicated with the inlet for nutrient solution and the outlet for metabolized waste solution of the cell cultivation tank. The cell supplying device is connected with the inlet for seed cells of the cell culture tank and the cell collecting device is connected with the outlet for cultured cells of the cell culture tank. The present invention has the advantages of simple structure, easy operation, single use or combined use of multiple devices, cell culture cost much lower than that of a combined method by singly adding exogenous cell factors.
Description
Technical field
The present invention relates to cell culture apparatus, particularly relate to a kind of can the cultivation rapidly in a large number, obtain the device of the daughter cell of specific cells, particularly stem cell and different differentiation degrees thereof external.
Background technology
Stem cell is regarded as the desirable seed cell of cell therapy, organizational project because of having self, multidirectional differentiation potential.Be that cell therapy or organizational project all need a considerable amount of stem cells, yet source of human stem cell is limited, complicated operation is cultivated on planes such as current common porous plate, square vase, growth microenvironment discontinuity changes huge, the cell growth influence is big, and the cell number of results does not reach clinical needs again far away.Simultaneously, in culture system, directly add combination of cytokines the cultivation cost is increased greatly, be difficult to push stem-cell therapy to clinical application.Therefore, suddenly need a kind of can be in external a large amount of culturing stem cells rapidly, and can significantly not increase the device of cultivating cost and come out.
Simultaneously, be induced to differentiate in the method for particular cell types (as neurocyte, muscle cell, insulin secretory cell, scleroblast, adipocyte etc.) present stem cell, also mostly be and add the microenvironment that external source chemical reagent, combination of cytokines are built the stem cell directional differentiation, cultivate cost according to height not under the time also exist various factor reagent to consume in time and appear at different time points and have concentration gradient, the shortcoming that inducible system is stable inadequately.Therefore, urgent need is a kind of can replenish the device that stem cell directional is induced the various factors of differentiation needs by endogenous in real time in culture system.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can obtain specific cells, particularly stem cell and can significantly not increase the cell culture apparatus of cultivating cost external a large amount of the cultivation rapidly.
For achieving the above object, the present invention takes technical scheme: a kind of cell culture apparatus, it comprises: a cell cultures jar, the one end is provided with seed cell inlet, nutritive medium inlet and a stroma cell and adds mouth, and its other end is provided with one and cultivates back cell outlet, a metabolism waste liquid outlet and a stroma cell relief outlet; Be provided with at least 2 nutritive medium supply pipes in this cell cultures jar, each nutritive medium supply pipe is a blind pipe, and near the end sealing of metabolism waste liquid outlet, and the other end and described nutritive medium inlet are connected; Be provided with a metabolism waste liquid recovery tube in this cell cultures jar, this metabolism waste liquid recovery tube is a blind pipe, and near the end sealing of nutritive medium inlet, and the other end and described metabolism waste liquid outlet are connected; Also be provided with at least 2 root cells culture tubes in this cell cultures jar, an end of each cell culture tube and seed cell inlet are connected, and cell outlet is connected after the other end and the cultivation; Elsewhere in this cell cultures jar is filled the suspension cell of expiring stroma cell, attached cell or direct growth; One nutritive medium circulation device, it connects the nutritive medium inlet and the metabolism waste liquid outlet of described cell cultures jar respectively; One cell generator, it connects the seed cell inlet of described cell cultures jar; One cell collection device, it connects cell outlet after the cultivation of described cell cultures jar.
In the above-mentioned cell culture apparatus, described nutritive medium circulation device includes a nutritive medium hold-up vessel, the liquid outlet of this nutritive medium hold-up vessel is connected with the nutritive medium inlet of described cell cultures jar by a nutritive medium supply pump, and the liquid return hole of this nutritive medium hold-up vessel is connected with the metabolism waste liquid outlet of described cell cultures jar by a metabolism waste liquid extraction pump; Described cell generator includes a seed cell hold-up vessel, and its outlet is connected with the seed cell inlet of described cell cultures jar by a cell supply pump; Described cell collection device includes a cell harvesting jar, its inlet by the cultivation of a cell harvesting pump and described cell cultures jar after cell outlet be connected.
In the above-mentioned cell culture apparatus, set up a cell detection device between described cell harvesting pump and the cell harvesting jar, this cell detection device is a control valve, its liquid outlet has two, one connects described cell harvesting jar, another is connected between described seed cell hold-up vessel and the cell supply pump, size when cell detection device pair cell, endochylema endoparticle situation, cell proportion detects when qualified, then with cell transfered cell holding tank, size when cell detection device pair cell, endochylema endoparticle situation, cell proportion detects when defective, then cell is led and get back to the nutritive medium supply pump, make it enter the cell cultures jar once more; The exit of described seed cell hold-up vessel also is provided with check valve.
In the above-mentioned cell culture apparatus, outside described metabolism waste liquid outlet, set up a metabolism waste liquid proofing unit.
In the above-mentioned cell culture apparatus, the tube wall of described nutritive medium supply pipe, metabolism waste liquid recovery tube and cell culture tube is a semi-permeable membranes.
In the above-mentioned cell culture apparatus, described nutritive medium supply pipe equidistantly is evenly distributed on the position of the outer wall of cell cultures jar, and it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described metabolism waste liquid recovery tube is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described cell culture tube then equidistantly is distributed between each nutritive medium supply pipe and the metabolism waste liquid recovery tube, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.
The present invention is owing to take above design, it has the following advantages: 1, cell culture apparatus provided by the present invention mainly adopts tubular fibre and microcarrier, simulated liver, marrow structure, can secrete the cell of the required factor of target cell with stroma cell or other, comprising that transgenic cell is cultivated altogether reproduces specific cell micro-environment in the body, can effectively breed and break up various stem cells, for the stem-cell therapy clinical application provides competent cell source, the frontier of opening up stem-cell therapy.2, the proofing unit that the pair cell size that is provided with among the present invention, endochylema endoparticle situation, cell proportion etc. carry out can judge whether cultivate the cell that comes out can reach requirement, if reach requirement then collect, if do not reach requirement then return subculture, this cell that has just guaranteed that the present invention finally collects meets requirements for clinical application fully.3, simple in structure, the processing ease of the present invention, both can use separately also can many covering devices applied in any combination, can use unified condition, also can form different environment in different units, can be different with the environment requirement expansion according to purpose, produce and amplify easily; Particularly the cost of cell cultures is compared simple external source and is added the method for combination of cytokines and reduce a lot, the present invention is beneficial to more promotes the use of.
Description of drawings
Fig. 1 is the structural representation of cell cultures jar among the present invention
Fig. 2 is the A-A sectional view of Fig. 1
Fig. 3 is an one-piece construction synoptic diagram of the present invention
Embodiment
Please arrange in pairs or groups Fig. 1, Fig. 2, shown in Figure 3 are a kind of cell culture apparatus provided by the present invention, and it mainly includes:
One cell cultures jar 1, one end are provided with seed cell inlet 11, one nutritive medium inlet 12 and one stroma cell and add mouth 5, and its other end is provided with one and cultivates back cell outlet 13, a metabolism waste liquid outlet 14 and a stroma cell relief outlet 6; Be provided with at least 2 nutritive medium supply pipes 15 in this cell cultures jar 1, each nutritive medium supply pipe 15 is a blind pipe, and near the end sealing of metabolism waste liquid outlet 14, and the other end and nutritive medium inlet 12 are connected; Be provided with a metabolism waste liquid recovery tube 16 in this cell cultures jar 1, metabolism waste liquid recovery tube 16 also is a blind pipe, and near the end sealing of nutritive medium inlet 12, and the other end and metabolism waste liquid outlet 14 are connected; Also be provided with at least 2 root cells culture tubes 17 in this cell cultures jar 1, an end of each cell culture tube 17 and seed cell inlet 11 are connected, and cell outlet 13 is connected after the other end and the cultivation; Elsewhere in this cell cultures jar 1, fill the suspension cell 18 that completely grows in stroma cell, other attached cells or direct growth on the microcarrier, the suspension cell 18 of these stroma cells, other attached cells or direct growth can enter or 6 discharges of stroma cell relief outlet by stroma cell interpolation mouth 5;
One nutritive medium circulation device 2, it connects the nutritive medium inlet 12 and the metabolism waste liquid outlet 14 of cell cultures jar 1 respectively;
One cell generator 3, it connects the seed cell inlet 11 of cell cultures jar 1;
One cell collection device 4, cell outlet 13 after the cultivation of its connection cell cultures jar 1.
In the present embodiment, specifically, nutritive medium circulation device 2 includes a nutritive medium hold-up vessel 21, the liquid outlet 211 of this nutritive medium hold-up vessel 21 is connected with the nutritive medium inlet 12 of cell cultures jar 1 by a nutritive medium supply pump 22, and the liquid return hole 212 of this nutritive medium hold-up vessel 21 is connected with the metabolism waste liquid outlet 14 of cell cultures jar 1 by a metabolism waste liquid extraction pump 23; Cell generator 3 includes a seed cell hold-up vessel 31, and its outlet 311 is connected with the seed cell inlet 11 of cell cultures jar 1 by a cell supply pump 32; Cell collection device 4 includes a cell harvesting jar 41, its inlet 411 by the cultivation of a cell harvesting pump 42 and cell cultures jar 1 after cell outlet 13 be connected.In addition, as required, can also between cell harvesting pump 42 and cell harvesting jar 41, set up a pair cell size, endochylema endoparticle situation, the device 43 that cell proportion etc. detects, this cell detection device 43 is a control valve, its liquid outlet has two, one connects cell harvesting jar 41, another is connected between seed cell hold-up vessel 31 and the cell supply pump 32, when cell detection device 43 detection cells are qualified, then with cell transfered cell holding tank 41, when cell detection device detection cell is defective, then cell is led and get back to nutritive medium supply pump 32, make it enter cell cultures jar 1 once more; For preventing that cell from flowing backwards in seed cell hold-up vessel 31, the special check valve 33 that is provided with in the exit of seed cell hold-up vessel 31.
In addition, a metabolism waste liquid proofing unit 24 can also be set outside metabolism waste liquid outlet 14, by its detection to the metabolism waste liquid, regulate the feed speed of nutritive medium supply pump 22, microenvironment is stable in the holding tube; The also detection by 24 pairs of metabolism waste liquids of metabolism waste liquid proofing unit, judge nutrient what it remains, when the residue nutrient is fewer, then can replenish nutrient to the nutritive medium in the nutritive medium hold-up vessel 21, perhaps all, part is changed the nutritive medium in the nutritive medium hold-up vessel 21, the nutritive medium nutrient abundance that assurance enters nutritive medium supply pipe 15.This metabolism waste liquid proofing unit 24 can be existing proofing unit or detection chip, and it can directly be buied from market, does not do too much at this and gives unnecessary details.
The tube wall of the nutritive medium supply pipe 15 in the foregoing description, metabolism waste liquid recovery tube 16 and cell culture tube 17 is semi-permeable membranes, can guarantee the normal nutrition supply of cell, the nutritive substances such as cytokine that the stroma cell secretion provides in the culture tank can be entered in the cell culture tube 17, the purpose cell is played a role; Provide pressure a little more than metabolism waste liquid extraction pump 23 swabbing pressures by nutritive medium supply pump 22, the coordination of associational cells supply pump 32, cell harvesting pump 42, can make to press in the culture tank and keep a little more than normal atmosphere, when preventing that seepage and external source from polluting, the part pot liquid is discharged by cell culture tube, can guarantee fully that the cytokine that the stroma cell secretion provides can abundant and purpose cytosis.
In the foregoing description, each nutritive medium supply pipe 15 equidistantly is evenly distributed on the position of cell cultures jar 1 outer wall, and it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Metabolism waste liquid recovery tube 16 is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; 17 of cell culture tubes equidistantly are distributed between each nutritive medium supply pipe 15 and the metabolism waste liquid recovery tube 16, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.Being provided with like this is beneficial to nutrient solution fully through being arranged at a jar inner cell culture tube, makes cell obtain the sufficient nutrition supply.
In the present embodiment, specifically, the tube wall diameter of nutritive medium supply pipe 15 is 200 μ m, and its quantity is 18; The tube wall diameter of metabolism waste liquid recovery tube 16 is 800 μ m; And the tube wall diameter of cell culture tube 17 is 500 μ m, and its quantity is 12.
The present invention is an example to cultivate embryonic stem cell in concrete the use:
The amplification in vitro of human embryo stem cell ES
50ml tubular fibre culture tank is used cobalt Co
60Radiation sterilization is used 1%H again
2O
2Sterilization, it is standby that sterilized water, PBS damping fluid wash twice back.In inoculated stromal cell the day before yesterday, connect each pipeline, will be full of serum-free medium in the culture tube, opening each circulation trial run, detect that ne-leakage is arranged and pollute may.
Behind microcarrier autoclaving, the substratum neutral equilibrium, with Co
60The stroma cell MEF of irradiation back forfeiture multiplication capacity is inoculated on the microcarrier; form microcarrier-stroma cell complex body; in application of sample mouth inoculation daughter cell culture tank; substratum is a serum free culture system, and by the control to nutritive medium, temperature is 35 ℃～38 ℃ in the adjustable pipe; the pH value is 7.1～7.5; oxygen concn is 0.01mmol/L～0.30mmol/L, regulates nutritive medium supply pipe and metabolism waste liquid recovery tube pressure, makes liquid in pipe so that speed is mobile very slowly.Cultivate in treating jar stable after, human embryo stem cell is inserted cell culture tube, the adjustable pipe internal pressure makes embryonic stem cell wriggle in culture tube with slow speed, propagation and keep its differentiation potential in its process.Detect the cytodifferentiation state at the cell harvesting pipe, cell space is become big, the tangible cells exclude of spontaneous differentiation is not broken up the ES cell cycle and is cultivated propagation.This culture cycle was 3～6 weeks.
Experiment shows, cultivates inoculation 5 * 10 when initial
5The ES cell cultivating through week age, can obtain 3 * 10
7ES cell, productive rate are 12.3 times that common square vase is cultivated.Flow cytometer detects, and obtain cell and all express Oct-4, SSEA-3, SSEA-4, TRA-1-61, the surface marker of ES cell-specifics such as telomerase activation, karyotyping is normal; The cell that obtains is transplanted to the teratoma that can form polyembryony layer source in the SCID mouse body.Above-mentioned detection shows that all the cell that obtains has kept the potential of multidirectional differentiation.
The present invention can also be by changing different stroma cells, even can not add stroma cell or the purpose cell directly carried out mode such as disposable cultivation outside tubular fibre ordinary cells is cultivated.As utilize co-culture of cells such as tumour cell and dendritic cell, T lymphocyte, and utilize the antigen activates immunocyte of tumour cell, making the cell that utilizes immuno-stimulating carry out the tumour cell treatment becomes possibility.
Claims (10)
1. cell culture apparatus is characterized in that it comprises:
One cell cultures jar, one end are provided with seed cell inlet, nutritive medium inlet and a stroma cell and add mouth, and its other end is provided with one and cultivates back cell outlet, a metabolism waste liquid outlet and a stroma cell relief outlet; Be provided with at least 2 nutritive medium supply pipes in this cell cultures jar, each nutritive medium supply pipe is a blind pipe, and near the end sealing of metabolism waste liquid outlet, and the other end and described nutritive medium inlet are connected; Be provided with a metabolism waste liquid recovery tube in this cell cultures jar, this metabolism waste liquid recovery tube is a blind pipe, and near the end sealing of nutritive medium inlet, and the other end and described metabolism waste liquid outlet are connected; Also be provided with at least 2 root cells culture tubes in this cell cultures jar, an end of each cell culture tube and seed cell inlet are connected, and cell outlet is connected after the other end and the cultivation; Elsewhere in this cell cultures jar is filled the suspension cell of expiring stroma cell, attached cell or direct growth;
One nutritive medium circulation device, it connects the nutritive medium inlet and the metabolism waste liquid outlet of described cell cultures jar respectively;
One cell generator, it connects the seed cell inlet of described cell cultures jar;
One cell collection device, it connects cell outlet after the cultivation of described cell cultures jar.
2. cell culture apparatus according to claim 1, it is characterized in that: described nutritive medium circulation device includes a nutritive medium hold-up vessel, the liquid outlet of this nutritive medium hold-up vessel is connected with the nutritive medium inlet of described cell cultures jar by a nutritive medium supply pump, and the liquid return hole of this nutritive medium hold-up vessel is connected with the metabolism waste liquid outlet of described cell cultures jar by a metabolism waste liquid extraction pump; Described cell generator includes a seed cell hold-up vessel, and its outlet is connected with the seed cell inlet of described cell cultures jar by a cell supply pump; Described cell collection device includes a cell harvesting jar, its inlet by the cultivation of a cell harvesting pump and described cell cultures jar after cell outlet be connected.
3. cell culture apparatus according to claim 2, it is characterized in that: set up a cell detection device between described cell harvesting pump and the cell harvesting jar, this cell detection device is a control valve, its liquid outlet has two, one connects described cell harvesting jar, another is connected between described seed cell hold-up vessel and the cell supply pump, size when cell detection device pair cell, endochylema endoparticle situation, cell proportion detects when qualified, then with cell transfered cell holding tank, size when cell detection device pair cell, endochylema endoparticle situation, cell proportion detects when defective, then cell is led and get back to the nutritive medium supply pump, make it enter the cell cultures jar once more; The exit of described seed cell hold-up vessel also is provided with check valve.
4. according to claim 2 or 3 described cell culture apparatus, it is characterized in that: outside described metabolism waste liquid outlet, set up a metabolism waste liquid proofing unit.
5. according to claim 1 or 2 or 3 described cell culture apparatus, it is characterized in that: the tube wall of described nutritive medium supply pipe, metabolism waste liquid recovery tube and cell culture tube is a semi-permeable membranes.
6. cell culture apparatus according to claim 4 is characterized in that: the tube wall of described nutritive medium supply pipe, metabolism waste liquid recovery tube and cell culture tube is a semi-permeable membranes.
7. according to claim 1 or 2 or 3 described cell culture apparatus, it is characterized in that: described nutritive medium supply pipe equidistantly is evenly distributed on the position of the outer wall of cell cultures jar, it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described metabolism waste liquid recovery tube is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described cell culture tube then equidistantly is distributed between each nutritive medium supply pipe and the metabolism waste liquid recovery tube, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.
8. cell culture apparatus according to claim 4, it is characterized in that: described nutritive medium supply pipe equidistantly is evenly distributed on the position of the outer wall of cell cultures jar, it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described metabolism waste liquid recovery tube is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described cell culture tube then equidistantly is distributed between each nutritive medium supply pipe and the metabolism waste liquid recovery tube, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.
9. cell culture apparatus according to claim 5, it is characterized in that: described nutritive medium supply pipe equidistantly is evenly distributed on the position of the outer wall of cell cultures jar, it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described metabolism waste liquid recovery tube is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described cell culture tube then equidistantly is distributed between each nutritive medium supply pipe and the metabolism waste liquid recovery tube, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.
10. want 6 described cell culture apparatus according to right, it is characterized in that: described nutritive medium supply pipe equidistantly is evenly distributed on the position of the outer wall of cell cultures jar, it is that the tubular fibre boundling of 200 μ m～1000 μ m is formed by diameter, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described metabolism waste liquid recovery tube is positioned at the central authorities of cell cultures jar, and its tubular fibre boundling by external diameter 200 μ m～800 μ m is formed, and its tube wall aperture is 0.01 μ m～0.1 μ m; Described cell culture tube then equidistantly is distributed between each nutritive medium supply pipe and the metabolism waste liquid recovery tube, and it is made of the tubular fibre boundling, and internal diameter is 100 μ m～500 μ m, and the tube wall small aperture is 0.5 μ m～5 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101344892A CN100376665C (en) | 2005-12-15 | 2005-12-15 | Cell culturation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101344892A CN100376665C (en) | 2005-12-15 | 2005-12-15 | Cell culturation apparatus |
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| CN1814745A CN1814745A (en) | 2006-08-09 |
| CN100376665C true CN100376665C (en) | 2008-03-26 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100848938B1 (en) * | 2007-04-20 | 2008-07-29 | 코아스템(주) | Cell culture tube and mass cell culture apparatus including the same |
| CN101130744B (en) * | 2007-07-26 | 2011-07-27 | 上海交通大学 | Airlift type bioreactor system for continuously pouring and culturing medicinal plant histiocyte |
| CN103146578B (en) * | 2013-03-29 | 2014-11-05 | 东北农业大学 | Transgenic cell culture device |
| JP6291429B2 (en) * | 2015-01-20 | 2018-03-14 | 富士フイルム株式会社 | Cell culture device and cell culture method |
| CN104893975B (en) * | 2015-05-28 | 2016-06-01 | 深圳富利鑫健康产业发展有限公司 | A kind of two room selective penetrated property cell culture apparatus and application thereof |
| CN105670993B (en) * | 2016-04-22 | 2022-04-01 | 福州创方医药科技有限公司 | Integrated T cell culture extraction method |
| CN106497784B (en) * | 2016-09-01 | 2019-03-22 | 奥凯（苏州）生物技术有限公司 | A kind of control pressurer system and its compress control method for realizing culture solution and waste liquid exchange |
| CN106635790B (en) * | 2016-09-01 | 2019-02-26 | 奥凯（苏州）生物技术有限公司 | A kind of fully enclosed automatic cell culture system |
| CN106497783B (en) * | 2016-09-01 | 2019-03-22 | 奥凯（苏州）生物技术有限公司 | A kind of dynamic circulation cell culture apparatus and cell culture processes |
| EP3556842A4 (en) * | 2017-01-20 | 2020-01-15 | FUJIFILM Corporation | Cell culture apparatus and cell culture method |
| CN107460115B (en) * | 2017-07-24 | 2021-08-31 | 大连理工大学 | Slow-release feed photobioreactor and microalgae culture method |
Citations (4)
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| CN2486557Y (en) * | 2001-01-19 | 2002-04-17 | 中国科学院化学研究所 | Cell cultivator |
| CN1435482A (en) * | 2002-01-31 | 2003-08-13 | 赛宇细胞科技股份有限公司 | Cell culture device |
| CN1471574A (en) * | 2000-10-24 | 2004-01-28 | ����ɽ�����ء������� | cell culture device |
| CN2861165Y (en) * | 2005-12-15 | 2007-01-24 | 中国人民解放军军事医学科学院野战输血研究所 | Cell cultivator |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471574A (en) * | 2000-10-24 | 2004-01-28 | ����ɽ�����ء������� | cell culture device |
| CN2486557Y (en) * | 2001-01-19 | 2002-04-17 | 中国科学院化学研究所 | Cell cultivator |
| CN1435482A (en) * | 2002-01-31 | 2003-08-13 | 赛宇细胞科技股份有限公司 | Cell culture device |
| CN2861165Y (en) * | 2005-12-15 | 2007-01-24 | 中国人民解放军军事医学科学院野战输血研究所 | Cell cultivator |
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