CN100345837C - Extraction of soya isoflavone, concentrated soya protein and soya oligose - Google Patents
Extraction of soya isoflavone, concentrated soya protein and soya oligose Download PDFInfo
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Abstract
The present invention discloses a technology for extracting soybean isoflavone, soybean concentrated protein and soybean oligosaccharide, which comprises: step (1), soybean meal is ground, and ground soybean meal powder are mixed with a solvent by the ratio of 1 to 10; the soybean meal powder and the solvent are extracted in the mode of ultrasonic circulation, and are filtrated by centrifugation so as to obtain extracting solution and soybean meal dregs; step (2), the extracting solution is concentrated, and the solvent is recovered; proteinase is added to the concentrated solution, and purified liquor and protein curd are obtained by winterization and centrifugation; step (3), the purified liquor is made to pass through a macroporous adsorptive resin bed to obtain water eluate and alcohol eluate; step (4), the alcohol eluate is concentrated, and ethanol is recovered; concentrated solution is dried by spray to obtain soybean isoflavone; step (5), soybean meal dregs and the protein curd are mixed, fried and pulverized to obtain soybean concentrated protein; step (6), water eluate is decolorized, filtrated and concentrated to obtain soybean oligosaccharide; step (7), the used processing accessory agent is recovered, and regenerated or purified for cyclic utilization. When soybean isoflavone is extracted in the present invention, the separated byproducts are used for preparing soybean concentrated protein and soybean oligosaccharide; acid and alkali wastewater and the used processing accessory agent are regenerated and purified for cyclic utilization; because waste is not exhausted, the technology is favorable to environmental protection and resource utilization.
Description
Technical field
The invention belongs to soybean deep processing field, particularly a kind ofly can rationally utilize its by product, and the technology of the extraction soybean isoflavones of no waste discharge and soybean protein concentrate, soybean oligosaccharide.
Background technology
Soybean isoflavones, soybean protein, soybean oligosaccharide are the important component in the soybean.
Soybean isoflavones is the contained main biologically active substance of soybean, and soybean isoflavones is the secondary metabolism product in the soybeans they grow, is present in plumular axis and the cotyledon.Present known soybean isoflavones has 12 kinds, comprises the glucosides of the former and 9 kinds of mating types of the glycosides of 3 kinds of free types.Soybean isoflavones can be converted into the compound that is similar to hormone by bacterium in the intestines, have oestrogenic hormon and anti-oxidant activity, in the breeding of malignant tumour, can block blood vessel hyperplasia effectively, break off the nutriment source, thereby delay or stop neoplastic disease to become cancer, can effectively suppress the generation of leukemia, colorectal carcinoma, lung cancer, cancer of the stomach, mammary cancer, prostate cancer.Simultaneously soybean isoflavones also has preventing osteoporosis, alleviates climacteric syndrome, regulating blood fat, prevent and treat cardiovascular disorder, medical health care function such as delay senility.Since extract the method complexity of soybean isoflavones, the cost height, and the price of domestic and international market is all high at present.
Soybean protein concentrate is rich in eight kinds of amino acid and other nonessential amino acid that human body is necessary such as Methionin, tryptophane, Xie Ansuan, soybean protein concentrate has characteristics such as water-retentivity, tackiness, gelation, oil absorbency and emulsifying property, can extensively be used as the basic material of numerous food, can also directly enter the consumption market, for the human consumer provides good vegetable-protein, soybean protein concentrate can also be widely used in feed processing, pharmacy, plastics industry field in addition.
Soybean oligosaccharide can not be absorbed in people's digestive tube, can be used as the sweeting agent that does not have energy uses, and soybean oligosaccharide can promote the propagation of intestinal beneficial bacterium, improve environment in the intestines, has defaecation, the anti-effect that treats constipation is widely used at food, restriction and medical health field.
Soybean isoflavones is many at present to be raw material with the dregs of beans, is solvent with certain density ethanol, methyl alcohol or water, adopts methods such as percolation process, counter-current extraction or ultrasonic broken wall extraction to extract, and the macroporous adsorbent resin method is separated and made.In some technology, soybean isoflavones is not main target product, only is one of extract.Chinese patent 00107606.X, patent name is " extracting the technology of soybean protein, isoflavones, saponin, oligose continuously ", Chinese patent 01128012.3, patent name all belongs to this type of for " the high and low temperature dregs of beans extracts the method for albumen, small peptide, nucleic acid, isoflavones, saponin, oligose ".Though the latter has extracted multiple composition, the production cycle is longer, and as only ultrasonic broken wall lixiviate just need be more than 6 hours, operation is numerous and diverse, though extract is many, the separation loss of energy consumption and each product is all higher.Other technologies are then only extracted soybean isoflavones, and by product is rationally utilized, and then give it up with, the water elution liquid that contains oligose for feed to be lower than value of the meal as the dregs of beans slag.This not only makes the production cost of soybean isoflavones higher, has also increased the environmental protection load.What more must pay close attention to is, using the macroporous adsorbent resin method at all separates in the technology of soybean isoflavones, common defects below also existing: the acid that resin regeneration produced, alkali waste water (at every turn producing the waste water of 3～4 times of material quantities approximately) do not purify, and can cause environmental pollution, increase expenses of environmental protection; Washing removal of impurities elutriant (organic liquid waste) does not purify, and can cause environmental pollution equally.Increase expenses of environmental protection; After extracting solution reclaims solvent, the purification of concentrated solution (deproteinated) processing many employings iso-electric point precipitation methods or calcium, magnesium salts condensation method, this not only takes off albumen not to the utmost, also certainly will increase desalination operation and brine waste.In sum, these technologies all do not meet the requirement of recycles such as environmental protection technology and water resources.
Summary of the invention
Purpose of the present invention provides a kind of technology of extracting soybean isoflavones and soybean protein concentrate, soybean oligosaccharide.It is when extracting soybean isoflavones; utilize isolating by product to produce soybean protein concentrate and soybean oligosaccharide; and, do not have waste discharge with the recycle after regeneration, purifying treatment of acid, alkali waste water and exhausted processing aid, help the environment protection and the utilization of resources.
The technical scheme that realizes the object of the invention is:
(1) dregs of beans is pulverized, the crushed bean powder carries out the ultrasonic circulating extraction with 70% ethanol by 1: 10 material ratio and obtains extracting solution and dregs of beans slag;
(2) the concentrated and recovery ethanol with described extracting solution adds 0.02～0.05% proteolytic enzyme (W/V) in concentrated solution, is incubated after abundant the stirring and leaves standstill, and with the continuous flow centrifuge separation, gets scavenging solution and albumen curd; Scavenging solution is separated with macroporous adsorbent resin, treats that resin absorption is near when saturated, use deionized water wash-out and 70% ethanol elution successively, water elution liquid and alcohol eluen; Alcohol eluen reclaims ethanol and concentrates, and concentrated solution spray-dried (inlet temperature is 125～130 ℃, and air outlet temperature is 80～85 ℃) obtains soybean isoflavones;
(3) dregs of beans slag and albumen curd combination drying are pulverized to moisture＜5% (reclaiming the adsorbed organic solvent of dregs of beans slag simultaneously) and with the micro mist pulverizer, obtained 350～400 purpose the concentrated albumen-powder of soybeans;
(4) in water elution liquid, add 2～2.5% powdered carbon and 1% diatomite W/V, under 85～90 ℃ condition, stir decolouring 30～40 minutes, be cooled to below 60 ℃ and filter, concentrate, obtain soybean oligosaccharide.
(5) to the in addition recycle of exhausted processing aid: ethanol utilizes after underpressure distillation is reclaimed again; Gac and diatomite cure through 630～640 ℃ of high temperature, treat that adsorbed organic matter carbonizing is complete, utilize after the cooling again; Acid, alkali waste water that macroporous adsorbent resin regeneration is produced with powdered carbon adsorption cleaning (consumption is 0.5～3%), utilize after the filtration again.
Advantage of the present invention is:
1, the present invention is primary product with the soybean isoflavones, rationally utilize isolating by product to produce soybean protein concentrate and soybean oligosaccharide, and efficient, practical apparatus and method fors such as ultrasonic circulating extraction, papoid condensation method deproteinated have been adopted, thereby technology is simple and direct, smooth, production cycle is shorter, and energy consumption and separation losses are lower.
2, the present invention has carried out utilizing after recycling and the purification as ethanol, gac, diatomite etc. to spent acid, alkali waste water and processing aid again, so no waste discharge, thereby can make full use of resource and help environment protection.
3 the present invention have realized that with lower technology cost the complete feed increment of dregs of beans utilizes and the recycle of related resource, thereby have the great potential that produces better economic benefit and social benefit.
4. the present invention is applicable to suitability for industrialized production, and its technology cost is relatively low.
The present invention is described further below in conjunction with embodiment.
Embodiment
One. extract soybean isoflavones
1. dregs of beans being pulverized with pulverizer is 20 purpose meal, and dregs of beans meal and 70% ethanol drop in the ultrasonic circulating extracting machine by 1: 10 solid-liquid ratio (W/V), at frequency 20～25KHz, and the sound intensity 1.5～2.0W/cm
2, the ultrasonic circulating extraction is 25～30 minutes under the condition that temperature is 35～40 ℃;
With the mixed liquor in the ultrasonic circulating extracting machine through centrifugal basket drier centrifuging (1500～2000 rev/mins) and dry filter residue, extracting solution and dregs of beans slag;
3. extracting solution is 0.08～0.09MPa with centrifugal-film evaporator in vacuum tightness, reclaims ethanol under the condition that temperature is 55～66 ℃ and is concentrated into 1.2～1.4 times (weight/volume) that are equivalent to material quantity, gets concentrated solution;
4. take by weighing the papoid that is equivalent to concentrate liquid measure 0.02～0.05% (weight/volume), contain in the insulation jar of concentrated solution with deionized water dissolving that is equivalent to 100 times of amounts of papoid and adding, fully stirring the back left standstill 30～50 minutes in 37～40 ℃ of insulations, separate (5500～6000 rev/mins) with continuous flow centrifuge then, get scavenging solution and albumen curd;
With scavenging solution by macroporous adsorbent resin bed (resin can be selected NKA-9, AB-8, D-101) soybean isoflavones by resin absorption, effluent liquid then contains soybean oligosaccharide, treat that resin absorption is near when saturated, earlier be eluted to effluent liquid solid content＜0.5% (analyzing) with compound microcapsule with deionized water, the back to effluent liquid isoflavone-containing＜0.01% (using spectrophotometric analysis), gets water elution liquid and alcohol eluen with 70% ethanol elution;
6. alcohol eluen uses centrifugal-film evaporator at vacuum tightness 0.08～0.09MPa, reclaim ethanol under the condition that temperature is 55～66 ℃, and be concentrated into solid content and reach 25～30%, spray-dried (125～130 ℃ of the inlet temperature of concentrated solution, 80～85 ℃ of air outlet temperatures), get the soybean isoflavones of content 45～60%, recovery rate reaches more than 90% of material content.
Two. produce soybean protein concentrate
1. the dregs of beans slag is mixed with albumen curd to drop in the rotary type Vacuumdrier that installs the condensation ware additional and be dried to moisture＜5%, (vacuum tightness 0.09～0.095MPa, 50～60 ℃ of temperature), reclaim the adsorbed ethanol of dregs of beans slag (be equivalent to approximately material quantity 25～30%) simultaneously, dry dregs of beans slag;
2. with the micro mist pulverizer dry dregs of beans ground-slag is broken to 350～400 orders, gets subalbous micro mist shape soybean protein concentrate, its protein content is equivalent to more than 70% of dry-matter;
Three. produce soybean oligosaccharide
1. in extracting soybean isoflavones, add 2～2.5% powdered carbon and 1% diatomite (weight/volume) in the 5th resulting water elution liquid of step (containing the effluent liquid of scavenging solution) by resin bed, under the condition of 85～90 ℃ of temperature, stir decolouring 30～40 minutes, be cooled to below 60 ℃, filter with diatomite filter, filter cake proper amount of deionized water filter wash, washing filtrate is incorporated in the filtrate, gets destainer;
2. be 0.08～0.09MPa with centrifugal-film evaporator in vacuum tightness with destainer, be concentrated into soluble solid content under 75～80 ℃ of conditions of temperature and reach 74～76%, obtain clear and bright melicera soybean oligosaccharide;
With soybean oligosaccharide slurry and dextrin in 2.5: 1 ratio mixing and add deionized water solid content is adjusted into 25～30%; carry out spraying drying then; can obtain the powdery soybean oligose of content about 60%, also soybean oligosaccharide dilution back can be cooperated an amount of dextrin to make granular soya oligose about 50% with the fluidized granulating machine.
Four. exhausted processing aid and recycle sour, alkali waste water
1. ethanol uses for next after transferring to 70% (V/V) concentration through the distillation recovery and with deionized water;
2. gac and diatomite will dry in the rearmounted High Temperature Furnaces Heating Apparatus in 630～640 ℃ through centrifugal basket drier and cure with crossing and having adsorbed organic gac and diatomite, treat that adsorbed organic matter carbonizing is complete, and the cooling back is for use next time;
3. in the alkaline waste water that macroporous adsorbent resin regeneration is produced, add and add 0.5～1% powdered carbon in 2～3% powdered carbon, the acid waste water, under room temperature, stirred 1～2 hour then, filter (the filter cake filter wash is the same) with diatomite filter respectively again, filtrate is for using next time.
Claims (6)
1. technology of extracting soybean isoflavones and soybean protein concentrate, soybean oligosaccharide is characterized in that:
(1) dregs of beans is pulverized, crushed bean powder and 70% ethanol are by 1: the material ratio of 10W/V drops into and carries out the ultrasonic circulating extraction in the ultrasonic circulating extracting machine, and centrifuging obtains extracting solution and dregs of beans slag;
(2) the concentrated and recovery ethanol with extracting solution adds proteolytic enzyme in the concentrated solution, and condensation, centrifugal gets scavenging solution and albumen curd;
(3) scavenging solution is passed through the macroporous adsorbent resin bed, water and 70% ethanol elution get water elution liquid and 70% ethanol eluate successively;
(4) the concentrated and recovery ethanol with ethanol eluate, concentrated solution is spray-dried, gets soybean isoflavones;
(5) with dregs of beans slag and albumen curd combination drying, pulverizing, soybean protein concentrate;
(6) water elution liquid gets soybean oligosaccharide through decolouring, filtration, concentrated;
(7) the exhausted processing aid is through reclaiming regeneration or the recycle that purifies.
2. the technology of extraction soybean isoflavones according to claim 1 and soybean protein concentrate, soybean oligosaccharide is characterized in that: the proteolytic enzyme in described (2) is papoid, and its consumption is 0.02～0.05%W/V of concentrated solution; Adopt the continuous flow centrifuge centrifugation.
3. the technology of extraction soybean isoflavones according to claim 1 and soybean protein concentrate, soybean oligosaccharide is characterized in that: the spray-dired inlet temperature of concentrated solution is 125～130 ℃ in described (4), and air outlet temperature is 80～85 ℃.
4. the technology of extraction soybean isoflavones according to claim 1 and soybean protein concentrate, soybean oligosaccharide, it is characterized in that: the combination drying in described (5) adopts the rotary type Vacuumdrier that installs condenser additional to carry out the dynamic vacuum drying, reclaims the adsorbed ethanol of dregs of beans slag simultaneously; Pulverize and adopt the micro mist pulverizer.
5. the technology of extraction soybean isoflavones according to claim 1 and soybean protein concentrate, soybean oligosaccharide, it is characterized in that: the decolouring in described (6) adopts gac and diatomite for mixing discoloring agent, stirs decolouring 30～40 minutes under 85～90 ℃ condition.
6. the technology of extraction soybean isoflavones according to claim 1 and soybean protein concentrate, soybean oligosaccharide is characterized in that: the recycle in described (7)
A. ethanol is through the underpressure distillation recycling;
B. gac and diatomite cure through 630～640 ℃ of high temperature, treat that adsorbed organic matter carbonizing is complete, utilize after the cooling again;
C. the acid, the alkali waste water that are produced of macroporous adsorbent resin regeneration is with the powdered carbon adsorption cleaning, filter the back for use next time.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100203276A CN100345837C (en) | 2005-01-31 | 2005-01-31 | Extraction of soya isoflavone, concentrated soya protein and soya oligose |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100203276A CN100345837C (en) | 2005-01-31 | 2005-01-31 | Extraction of soya isoflavone, concentrated soya protein and soya oligose |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007068143A1 (en) * | 2005-12-13 | 2007-06-21 | Lanqin Yang | Preparation of plant oil, crude protein and fiber by water extraction |
| CN101029320B (en) * | 2007-04-09 | 2011-04-13 | 重庆理工大学 | Method for extracting bean mill cake isoflavone by cellulase synergistic supersonic wave |
| CN101712715A (en) * | 2009-12-18 | 2010-05-26 | 周鑫 | Method for decolorizing full water-soluble soybean glycoprotein |
| CN101845077B (en) * | 2010-05-17 | 2012-10-03 | 天津实发中科百奥工业生物技术有限公司 | Preparation technology of soybean protein concentrate by ultrasonic continuous countercurrent leaching alcohol method |
| CN102002029B (en) * | 2010-11-06 | 2012-01-11 | 黑龙江八一农垦大学 | Method for separating and extracting isoflavone by simulated moving bed adsorption |
| CN102696766A (en) * | 2012-06-12 | 2012-10-03 | 上海大学 | Preparation method for soybean health drink |
| CN102726573A (en) * | 2012-07-04 | 2012-10-17 | 北京绿源求证科技发展有限责任公司 | Health tea granule for preventing dyslipidemia |
| CN102838575B (en) * | 2012-08-30 | 2014-08-20 | 东北农业大学 | Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments |
| CN103734452A (en) * | 2013-12-27 | 2014-04-23 | 临邑禹王植物蛋白有限公司 | Production method of high-gel soy protein concentrate |
| CN103896994B (en) * | 2014-03-11 | 2017-01-18 | 黑龙江八一农垦大学 | Method for separating and extracting oligosaccharide from soybean whey waste liquid |
| CN106282258A (en) * | 2016-09-24 | 2017-01-04 | 合肥信达膜科技有限公司 | A kind of soybean isoflavone isolation technics |
| CN117623884B (en) * | 2023-11-30 | 2025-08-08 | 安徽大学绿色产业创新研究院 | Method for extracting D-pinitol, isolated soy protein and soy isoflavone by comprehensively utilizing soybean meal |
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