CN100339014C - Application of N-acetylglucosamine in dairy product and dairy product containing N-acetylglucosamine - Google Patents

Abstract

The invention discloses the application of N-acetylglucosamine as an additive in dairy products and a novel dairy product containing N-acetylglucosamine. The new dairy product produces new beneficial effects on the body, and the effect is remarkable in crowd trials.

Description

Application of N-acetylglucosamine in dairy products and dairy products containing N-acetylglucosamine

Technical field

The present invention relates to the application of N-acetylglucosamine or its acceptable salt for dairy products as an additive in dairy products, and dairy products containing N-acetylglucosamine or its acceptable salt for dairy products.

Background technique

Dairy products are rich in nutrients, widely used, closely related to human survival, and become the main object of nutrition research. The formation of new biological theories is naturally also related to dairy products. Biological wave theory is a new theory of biology. It is the latest biological theory established and confirmed through experimental research on the basis of the latest progress in the field of contemporary physics and mathematics—complexity exploration, combined with the actual situation of biology and medicine (see, " Research on Biological Wave Theory", Chinese Journal of Microecology, 1994, 6(6): 40-49). The biological wave theory believes that the human body is an open system, and the metabolic process promotes biological dissipation and ecological unity, and performs the functions of life activities. Applying the biological wave theory to nutrition, the point of view that the condition of nutrient utilization is determined, that is, biological wave regulation factors promote the absorption and utilization of nutrients.

FR2101030 discloses a complex comprising porcine gastric mucin hydrolyzate and 15% w/w N-acetyl-D-glucosamine. The complex is a growth factor of Lactobacillus Bifidus and is used as a dietary supplement for infants fed cow's milk, thus providing a diet with effects similar to human milk and establishing a normal intestinal flora.

After research, the present inventor surprisingly found that N-acetylglucosamine or its milk product acceptable salt is used as a new type of dairy product prepared as a dairy product additive. Compared with traditional dairy products, it has the characteristics of fast absorption and good effect .

Contents of the invention

One object of the present invention is to provide the application of N-acetylglucosamine or its acceptable salt for dairy products as an additive in dairy products.

Another object of the present invention is to provide a dairy product comprising N-acetylglucosamine or a dairy acceptable salt thereof.

Another object of the present invention is to provide a method for preparing dairy products, which includes the step of adding N-acetylglucosamine or a dairy acceptable salt thereof to raw milk products.

The full name of the N-acetylglucosamine is N-acetyl-D-glucosamine (N-acetyl-D-glucosamine), the molecular formula is C ₈ H ₁₅ NO ₆ , and the structural formula (I) is as follows:

Examples of dairy-acceptable salts of N-acetyl-D-glucosamine that may be used in the present invention include, but are not limited to, those salts with inorganic acids, such as hydrochloride, phosphate, hydrogen phosphate, and those with organic acids. Those salts formed by acids such as citrate, benzoate, ascorbate.

There is virtually no restriction on the dairy raw materials that can be used in the present invention and the finished dairy products of the present invention. The dairy product of the present invention can be any form of dairy product that people usually eat in life, for example, it can be liquid such as liquid milk; solid such as milk powder and cheese; semi-solid such as yogurt and ice cream. The dairy products can be ordinary dairy products, or various nutritionally fortified dairy products such as calcium-added milk and the like.

There is also virtually no restriction on the way in which N-acetyl-D-glucosamine or its dairy product-acceptable salts can be added to dairy products, as long as the method of addition is effective for N-acetyl-D-glucosamine or its dairy products. Acceptable salts and other active ingredients in dairy products have no adverse effects.

In one embodiment of the present invention, the N-acetyl-D-glucosamine or a dairy acceptable salt thereof can be added to milk powder in the form of powder.

In another embodiment of the present invention, the N-acetyl-D-glucosamine or its acceptable salt for dairy products can be added to the liquid milk, and then the milk is sold as it is, or further adopt known methods in the art. Prepared with advanced technology such as milk powder, yogurt, etc.

The content of N-acetylglucosamine or its acceptable salt for dairy products in dairy products is generally 0.001g/100ml to 10g/100ml. Preferably it is 0.01 g/100ml to 2g/100ml. Based on the weight of solid effective substances in dairy products, the content of N-acetylglucosamine or acceptable salts thereof in dairy products is generally 0.1-10%, preferably 0.1-2%.

The novel dairy product containing N-acetylglucosamine or its acceptable salt for dairy products of the present invention has a new beneficial effect on the body, and the effect is remarkable in crowd trials.

Detailed ways

The present invention and its beneficial effects will be further described below in conjunction with the examples, but it should be understood that these examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention in any way.

Embodiment 1. The wave-promoting test of the compound of formula (I)

1. Test materials and methods:

1.1 Sample: pure compound of formula (I).

1.2 Test material:

Strains: Proteus mirabilis should meet the following biochemical reaction characteristics: motility (+), urease (+), lactose (-), glucose (+), H ₂ S (-), phenylalanine deaminase (+ ).

Medium: modified LB medium (composition: 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 0.1% glucose, 0.002% TTC, pH7.2-7.4).

1.3 Test method:

Control sample: plant Proteus mirabilis in the center of the LB plate and culture at 37°C for 9 hours;

Test sample: Add the compound of formula (I) at a final concentration of 0.5% to the LB plate, plant Proteus mirabilis in the same way, and culture at 37° C. for 9 hours.

2. Test results and evaluation:

In the control sample, there are concentric rings that continuously expand outwards at intervals of 3 hours; in the test sample, not only the concentric rings that appear every 3 hours are formed, but also many small rings are displayed on each ring compared with the control sample. Volatility ring.

This test adopts the biological wave fluctuation test model to study the wave-promoting effect of the compound of formula (I). The results show that the compound of formula (I) can not only make the bacterial cells exhibit normal biological wave characteristics, but also make this fluctuation show more subtle Fluctuation mode, shows that the compound of formula (I) has promoting effect to biological fluctuation. The wave-promoting effect of the compound of formula (I) helps to explain the effect of N-acetylglucosamine on promoting the absorption of active ingredients in dairy products.

The toxicological test of embodiment 2. formula (I) compound

Carry out the toxicology test of formula (I) compound, comprise:

1. Acute toxicology test: including oral, intravenous injection and maximum dose administration test;

2. Ames test;

3. Mouse bone marrow cell micronucleus test;

4. Mouse sperm teratogenic test;

5. Chromosomal aberration test of mouse testis;

6. Chronic lethal test;

7. Subchronic toxicity (90-day feeding) test;

8. Traditional teratogenicity test;

Test conclusion shows: when formula (I) compound acute toxicity test dose exceeds 2g/kg, acute poisoning reaction does not yet occur; In long-term toxicity test, the highest dose has reached 1g/kg, through four weeks test observation, no toxic reaction occurs; In the reproduction test, mice were fed from a conventional dose of 7 mg/kg, and after three passages, it was proved that the compound of formula (I) had no effect on the conception, pregnancy, childbirth, lactation and development of offspring of mice. These tests demonstrate that the compound of formula (I) is a non-toxic substance.

Embodiment 3. Animal test

(1) increase appetite

Kunming mice were used for the experiment. The control group was orally gavaged with milk, and N-acetylglucosamine (addition amount: 0.1g/100ml milk) was added to the milk of the test group. As a result, it was observed that the foraging cycle of the mice in the test group was shortened, and more food was crushed (representing that the animals were actively foraging). ), increased food intake, and significantly increased body weight.

(2) Promote the development of suckling mice

The Kunming mice were used for the experiment, and the pregnant female mice were firstly taken and divided into a test group and a control group. The test group was orally gavaged the dairy product of the present invention (in the commercially available milk powder, N-acetylglucosamine 0.1g/100g milk powder was added), and the control group was orally gavaged with the commercially available milk powder, and were fed until the suckling mice were born and suckled for 15 days, and the two groups were weighed. The average body weight of offspring mice was significantly higher in the experimental group than in the control group.

Example 4. Preliminary population trial trial

36 voluntary population experiments showed that compared with drinking ordinary milk, those who drank the dairy product of the present invention (adding N-acetylglucosamine 0.1g/100ml in the sterilized fresh milk) reported increased appetite and good appetite, especially those with gastrointestinal When you are sick, the effect is more obvious. After drinking, you feel that your physical strength will recover quickly, your actions will be strong, and your mental state will be good. The effective rate is over 90%.

Embodiment 5. the preparation embodiment of dairy product of the present invention

1. Add N-acetylglucosamine into milk in solid form to prepare liquid milk with an N-acetylglucosamine content of 0.1g/100ml.

2. Add N-acetylglucosamine hydrochloride in solid form to milk to prepare liquid milk with an N-acetylglucosamine content of 0.1 g/100ml.

3. Adding N-acetylglucosamine into milk powder in solid form to prepare solid milk powder with an N-acetylglucosamine content of 0.2 g/100 g.

4. Adding N-acetylglucosamine in solid form to the fermented yogurt to prepare yogurt with an N-acetylglucosamine content of 0.1 g/100ml.

5. Add N-acetylglucosamine citrate into milk in the form of 10% by weight aqueous solution to prepare liquid milk with a final content of N-acetylglucosamine of 0.1 g/100 ml.

The observation of the above dairy products found that the addition of N-acetylglucosamine or its salt did not have a significant impact on the appearance of the dairy products.

Claims (7)

1.N-acetylglucosamine or the acceptable salt of its dairy products are as the application of additive in dairy products, wherein do not comprise the application in the dairy products of being combined in of N-acetylglucosamine and HGM hydrolysate, and described dairy products not baby foods.

2. the application of claim 1, wherein N-acetylglucosamine or the acceptable salt of its dairy products add in the powder dairy products.

3. the application of claim 1, wherein N-acetylglucosamine or the acceptable salt of its dairy products add in the liquid diary product.

4. each application among the claim 1-3, wherein N-acetylglucosamine or the content of the acceptable salt of its dairy products in dairy products are 0.001g/100ml to 10g/100ml.

5. comprise the dairy products of N-acetylglucosamine or the acceptable salt of its dairy products, described dairy products do not comprise the HGM hydrolysate, and described dairy products are not baby foods.

6. the dairy products of claim 5, wherein N-acetylglucosamine or the content of the acceptable salt of its dairy products in dairy products are 0.001g/100ml to 10g/100ml.

7. a method for preparing dairy products comprises the step that adds N-acetylglucosamine or the acceptable salt of its dairy products in the raw milk goods, do not comprise the HGM hydrolysate in the dairy products that wherein obtain, and described dairy products is not baby food.