CN109984365A - A kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform - Google Patents

Abstract

The invention discloses the use of a biotinylated pullulan polysaccharide derivative for improving the biotin transfer rate of cigarette cut tobacco. Compared with the added biotin monomer, the biotinylated pullulan polysaccharide derivative added in the cut tobacco of the present invention is more resistant to high temperature and can adapt to the high temperature thermal processing process. The biotinylated pullulan polysaccharide derivative added to the cut tobacco can be Significantly improve the biotin transfer rate of cut tobacco. At the same time, the biotin transfer rate of the mainstream smoke produced by heating the cigarettes rolled from the cut tobacco of the biotinylated pullulan derivative added in the present invention is high, which truly enhances the biotin functional properties of the cigarettes.

Description

A biotinylated pullulan derivative for improving biotin conversion of cigarette cut tobacco The use of mobility

technical field

The invention belongs to the application field of tobacco industry, in particular to the use of a biotinylated pullulan polysaccharide derivative for improving the biotin transfer rate of cigarette cut tobacco.

Background technique

Biotin is one of the B vitamins, also known as vitamin H, vitamin B7, coenzyme R (Coenzyme R), with a molecular weight of 244Da. Nutrients necessary for healthy human function. Adding biotin to the shredded cigarette can make the smoke produced by the rolled cigarettes have biotin, so that the cigarettes have the functional properties of biotin. At present, domestic cigarette companies mainly increase the content of biotin in cut cigarettes by adding biotin monomer to cut tobacco. However, from the actual situation, the cut tobacco after adding biotin monomer can obtain cigarette cigarettes after thermal processing and rolling. After smoking, the biotin transfer rate in the cut tobacco of the cigarette is not high, and the biotin transfer rate in the mainstream smoke of the cigarette during the smoking process is low, wherein, the biotin transfer rate in the cut tobacco = the content of Biotin content/the biotin content of the cut tobacco x 100%, the biotin transfer rate in the mainstream smoke = the biotin content in the mainstream smoke produced by the burning of the cut tobacco/the biotin content in the cut tobacco before the burning of the cut tobacco × 100%.

Therefore, it is hoped that cigarettes have good biotin functional properties. The most fundamental thing is to investigate the biotin content in the cut tobacco, not the biotin content in the cut tobacco after adding biotin monomer, but the appearance of cigarettes. The reason why the biotin content in the cut tobacco is not high may be that in the actual production process, after adding biotin to the cut tobacco, it is not directly used for cigarette rolling, but is subjected to high temperature (130-180 ℃) Therefore, whether the biotin added in the shredded tobacco can withstand high temperature and whether it can adapt to the high temperature thermal processing process is an important reason for the level of biotin retained in the shredded tobacco of rolled cigarettes.

Therefore, it is particularly important to develop a method for the biotin transfer rate of cigarette cut tobacco for the cigarette industry.

SUMMARY OF THE INVENTION

The present invention provides the use of a biotinylated pullulan polysaccharide derivative for improving the biotin transfer rate of cigarette cut tobacco, and the preparation method of the biotinylated pullulan polysaccharide derivative comprises the following steps:

(1) Add anhydrous dimethyl sulfoxide, biotin, dicyclohexylcarbodiimide and 4-dimethylaminopyridine in turn to the conical flask, react at room temperature for 0.5-1h, and then add pullulan , continue to react at room temperature for 0.5-1h, until the white turbidity is precipitated to obtain a turbid solution;

(2) filter the turbid solution obtained in step (1), filter and remove the white turbidity, collect the filtrate and put it into a dialysis bag with a molecular weight cut-off of 9000Da, add deionized water for dialysis for 4-8d, and replace it every 0.5h. Ionized water, after the dialysis, suction filtration to obtain a white precipitate, which is washed with dichloromethane and deionized water in turn, and dried to obtain a biotinylated pullulan derivative.

Preferably, in step (1), the molar ratio of anhydrous dimethyl sulfoxide, biotin, dicyclohexylcarbodiimide and 4-dimethylaminopyridine is 704:10:20:1.

Preferably, in step (1), the molar ratio of biotin to pullulan is 1/2-1.

Preferably, a biotinylated pullulan derivative is added to the cut tobacco, and the mass of the added biotinylated pullulan derivative accounts for 1/10 to 1/15 of the mass of the cut tobacco.

Wherein, the cut tobacco added with the biotinylated pullulan derivative is dried in an oven at 100 to 150° C. for 5 to 10 minutes to keep the moisture content of the cut tobacco at 12±1%, and then placed at a temperature of 22±1° C. Equilibrate in a humidity environment of 60±2% for 24-48 hours, and then carry out high-temperature drying process. Weigh 25g of balanced cut tobacco and place it in an oven at 150-180°C for 5-10 minutes to obtain cut tobacco for cigarettes. Among them, placing the balanced cut tobacco in an oven at 150-180°C for 5-10 minutes is to check whether the biotin added to the cut tobacco can withstand high temperature and whether it can adapt to the high-temperature thermal processing process.

Wherein, the biotin content in the sample is determined by high performance liquid chromatography, and the biotin transfer rate of the cut tobacco = the content of biotin contained in the cut tobacco/the content of biotin added to the cut tobacco x 100%. Then, the shredded tobacco that has undergone the high-temperature drying process is rolled into cigarettes, heated with a heat-not-burn cigarette, and the mainstream smoke generated by the cigarettes is collected. The content of biotin contained in the flue gas/the content of biotin remaining in the cut tobacco before the cut tobacco is burned × 100%.

Compared with the prior art, the present invention has the following beneficial effects:

1. Compared with the added biotin monomer, the biotinylated pullulan derivative added in the cut tobacco of the present invention is more resistant to high temperature and can adapt to the high temperature thermal processing process. After the high temperature drying process, the biotin monomer is added. The biotin transfer rate in the cut tobacco is only 38.02%, while the biotin transfer rate in the cut tobacco with biotinylated pullulan derivatives is 89.34%. Improve the biotin transfer rate of cut tobacco.

2. The biotin transfer rate in the mainstream smoke produced by heating the cigarettes made from the cut tobacco of the biotinylated pullulan derivative added in the present invention is the tobacco cut made of the added biotin monomer. The transfer rate of biotin in the mainstream smoke produced by the heating of the cigarette is 1.59 times higher, and the biotin in the shredded tobacco is not destroyed during the heating process of the cigarette of the present invention, but is volatilized with the mainstream smoke. Biotin is a necessary nutrient to maintain the natural growth, development and normal function of the human body. The increase in the transfer rate of biotin in mainstream smoke is the real enhancement of the biotin functional characteristics of cigarettes.

Description of drawings

none

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

Comparative Example 1

Take 100g of cut tobacco and evenly add 10g of biotin monomer, and dry it in an oven at 100 to 150°C for 5 to 10 minutes to keep the moisture content of the cut tobacco at 12±1%, then place it at a temperature of 22±1°C and a relative humidity of 60±2 % in the environment for 24-48 hours, and then carry out the high-temperature drying process. Weigh 25g of the balanced cut tobacco and place it in an oven at 150-180°C for 5-10 minutes to obtain a cut tobacco comparative sample 1.

Example 1

Take 100g of cut tobacco and evenly add 10g of biotinylated pullulan derivatives, and dry it in an oven at 100 to 150°C for 5 to 10 minutes to keep the moisture content of the cut tobacco at 12±1%, then place it at a temperature of 22±1°C, Equilibrate in an environment of relative humidity of 60±2% for 24-48 hours, and then carry out high-temperature drying process. Weigh 25g of balanced cut tobacco and place it in an oven at 150-180°C for 5-10 minutes to obtain cut tobacco sample 1.

Wherein, the preparation method of biotinylated pullulan derivatives is as follows: 100 mL of anhydrous dimethyl sulfoxide, 2 mmol of biotin, 2.4 mmol of dicyclohexylcarbodiimide and 0.2 mmol of 4-dimethyl sulfoxide are sequentially added to a 200 mL conical flask. Methylaminopyridine, react at room temperature for 0.7h, then add 4mmol pullulan, continue to react at room temperature until white turbidity is precipitated, remove the white turbidity by filtration, and put the filtrate into a dialysis bag with a molecular weight cut-off of 9000Da , add 3L deionized water for dialysis for 6d, and replace the dialysate every 0.5h. After the dialysis, the white precipitate was filtered with suction, the white solid obtained on the filter paper was washed with dichloromethane and deionized water in turn, and the product in the form of a white powder after drying was the biotinylated pullulan derivative.

The biotin content in the cut tobacco comparative sample 1 and cut tobacco sample 1 was determined by high performance liquid chromatography. Before the high temperature drying process, the biotin content in the comparative sample 1 and cut tobacco sample 1 was the same, and recorded as 100.00%. After the drying process, the biotin transfer rate of the cigarette cut tobacco = the biotin content contained in the cigarette cut tobacco/the cigarette cut tobacco content of the same quality plus the biotin content × 100%. The biotin transfer rates of different cut tobacco samples are shown in Table 1.

Then, the shredded tobacco sample 1 and the shredded tobacco sample 1 were rolled into cigarettes, and the cigarette reference sample 1 and the cigarette sample 2 were obtained. Collect mainstream smoke produced by cigarettes, biotin transfer rate in mainstream smoke = sum of biotin content in mainstream smoke produced by shredded cigarettes / shredded tobacco with the same quality as cigarette cigarettes plus biotin Content × 100%. The biotin transfer rates of mainstream smoke produced by different cigarette samples are shown in Table 2.

Table 1 Biotin transfer rate of different cut tobacco samples

It can be seen from Table 1 that the biotin transfer rate in the cut tobacco after adding the same mass of biotin monomer and biotinylated pullulan derivative has no significant difference before the high temperature drying process, but after high temperature After the drying process, the biotin transfer rate in the tobacco cut sample 1 added with biotin monomer was only 38.02%, while the biotin transfer rate in the tobacco cut sample 1 with biotinylated pullulan derivatives was 89.34%, and The biotin transfer rate of cut tobacco is closer to that before the high-temperature drying process, so the biotinylated pullulan derivatives added to the cut tobacco are more resistant to high temperature and can adapt to the high-temperature thermal processing process. Vegetated pullulan derivatives can improve the biotin transfer rate of cut tobacco.

Table 2 Biotin transfer rate of mainstream smoke produced by different cigarette samples

sample Biotin transfer rate (%) Cigarette comparison sample 1 8.04 Cigarette sample 1 12.79

As can be seen from Table 2, the biotin transfer rate in the mainstream smoke generated by the heating of the cigarette sample 1 of the present invention is 12.79%, that is, in the process of heating the cigarette sample 1 to generate smoke, 12.79% of the tobacco sample 1 has Biotin is volatilized with the smoke, and the biotin transfer rate in the mainstream smoke generated by the heating of the cigarette sample 1 of the present invention is 1.59 times that of the mainstream smoke generated by the heating of the cigarette control sample 1, that is to say In the process of heating the cigarettes of the present invention to generate flue gas, the biotin retained in the cut tobacco is not destroyed, but is volatilized with the mainstream flue gas. Biotin functional properties of branches.

Claims (3)

1. a kind of biotinylation pulullan polysaccharide derivative is used to improve the purposes of the cigarette shreds biotin rate of transform, feature exists In, the biotinylation pulullan polysaccharide derivative preparation method the following steps are included:

(1) anhydrous dimethyl sulphoxide, biotin, dicyclohexylcarbodiimide and 4- dimethylamino are sequentially added in conical flask Pyridine reacts 0.5-1h at room temperature, and pulullan polysaccharide is then added, and 0.5-1h is reacted in continuation at room temperature, until white casse Object is precipitated, and obtains turbid solution；

(2) turbid solution obtained in filtration step (1), white casse object is filtered to remove, and is collected filtrate and is put into retention molecule Amount is that deionized water dialysis 4-8d is added in the bag filter of 9000Da, replaces a deionized water every 0.5h, dialysis terminates Afterwards, it filters and obtains white precipitate, successively wash white precipitate with methylene chloride and deionized water, it is dry, it is general to obtain biotinylation Shandong orchid polysaccharide derivates.

2. the method according to claim 1, wherein in step (1), anhydrous dimethyl sulphoxide, biotin, two rings The molar ratio of hexyl carbodiimide and 4-dimethylaminopyridine is 704:10:20:1.

3. the method according to claim 1, wherein in step (1), the molar ratio of biotin and pulullan polysaccharide For 1/2-1.