CN109839375A - A kind of paper substrate micro-fluidic chip and detection method for examination of glucose concentration - Google Patents
A kind of paper substrate micro-fluidic chip and detection method for examination of glucose concentration Download PDFInfo
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- CN109839375A CN109839375A CN201910094129.6A CN201910094129A CN109839375A CN 109839375 A CN109839375 A CN 109839375A CN 201910094129 A CN201910094129 A CN 201910094129A CN 109839375 A CN109839375 A CN 109839375A
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Abstract
本发明基于颜色带长度变化的可用于葡萄糖浓度检测的纸基微流控芯片。不同浓度的葡萄糖溶液在条状的梯度阵列与楔形通道所构成的润湿梯度的自驱动型纸基微流控芯片的检测流道内所形成的颜色带长度的不同这一性质，建立葡萄糖浓度和其在条状的梯度阵列与楔形通道所构成的润湿梯度的自驱动型纸基微流控芯片上流动的颜色带长度一一对应的标准库。在需要检测葡萄糖浓度时，用滴管吸取待检测的葡萄糖溶液。测量其在检测流道内所形成的颜色带长度，并将这一长度与建立的葡萄糖标准库中的颜色带长度进行对比。匹配出相同长度的葡萄糖浓度的颜色带长度。即可检测出待测葡萄糖溶液的浓度。该方法操作简单，便捷经济，且可以较准确的检测出葡萄糖溶液的浓度。
The present invention is a paper-based microfluidic chip that can be used for glucose concentration detection based on the change of the length of the color band. The difference in the length of the color bands formed in the detection channel of the self-driven paper-based microfluidic chip of the self-driven paper-based microfluidic chip composed of the strip-shaped gradient array and the wedge-shaped channel of the glucose solution with different concentrations establishes the relationship between the glucose concentration and the wedge-shaped channel. It is a standard library of one-to-one correspondence to the lengths of the color bands flowing on the self-driven paper-based microfluidic chip of the wetting gradient formed by the strip-shaped gradient array and the wedge-shaped channel. When it is necessary to detect the glucose concentration, use a dropper to draw the glucose solution to be detected. Measure the length of the color band formed in the detection flow channel, and compare this length with the length of the color band in the established glucose standard library. Match out the lengths of the color bands for glucose concentrations of the same length. The concentration of the glucose solution to be tested can be detected. The method is simple to operate, convenient and economical, and can more accurately detect the concentration of the glucose solution.
Description
Technical field
The present invention relates to a kind of paper substrate micro-fluidic chips and detection method for examination of glucose concentration.
Background technique
Surge in Past 30 Years according to domestic diabetes morbidity is investigated, the abnormal people of blood glucose has 2.64 hundred million at present.
Glucose is indispensable substance in human life activity, and content is to measure metabolic capabilities and monitoring disease state of an illness shape
The important indicator of condition, especially in the diagnosis of clinical diabetes, the real-time monitoring of blood sugar concentration is the weight for controlling conditions of patients
Want means.Currently, the measuring method of glucose mainly has spectrophotometry, electrochemical process and high performance liquid chromatography etc., but this
In the presence of analysis speed, certain limitation such as more difficult is detected at relatively slow, higher cost, scene to a little technologies immediately.Therefore, it establishes fast
The glucose sensing approach of fast simple, economic accurate, live at-once monitor, can effectively solve the reality in existing glucose detection
Problem monitors important in inhibiting and wide application prospect to the prevention of human health and disease, diagnosing and treating.
Paper substrate micro-fluidic chip energy with unique advantages such as simple and convenient, easy processing, low cost, micromation, portability
Enough accomplish the primary prevention of diabetes, mid-term control and anaphase.The present invention is based on the gradient array of strip and wedgy passages
The color strip length of the self-driven type paper substrate micro-fluidic chip combination different glucose flowing of the wetting gradient constituted becomes
Change realizes it in micro- detection of concentration of glucose.Face is formed by detection channel according to the glucose solution of various concentration
The difference of colour band length can intuitively distinguish the size of concentration.In addition, due to combining wedge structure and Surface Energy Gradients, liquid
Drop in runner will there is bigger driving force to be easier to realization system to simplify the ancillary equipments such as external pressure system
Micromation and portability.
Summary of the invention
For existing problem, the present invention propose a kind of paper substrate micro-fluidic chip for examination of glucose concentration and
Detection method, this method can go out the concentration of glucose solution according to the accurate detection that color strip length changes, need not rely on
More equipment.
The purpose of the present invention is what is be achieved through the following technical solutions: a kind of paper base miniflow for examination of glucose concentration
Chip is controlled, the paper substrate micro-fluidic chip is that following base chips are carried out dyeing to handle to obtain, and the base chip includes wedge
Shape runner, the key groove of cuniform channel are 4 °；The direction of runner are as follows: from relatively narrow initial end to wider end；The wedge shape
There is functional pattern, the functional pattern is divided into multiple sectional areas along runner direction inside runner；In each sectional area
Functional pattern is formed by the hydrophilic pattern of several belt-like zones and the hydrophobic pattern alternative splicing of several strips；The strip
Hydrophobic pattern about cuniform channel jackshaft bilateral symmetry, the hydrophobic pattern and cuniform channel of the strip of each sectional area
Central axial direction it is parallel；From the beginning of cuniform channel to end, the width of the hydrophobic pattern of strip is constant, two neighboring to dredge
Spacing between water patterns is gradually increased, and strip hydrophobic pattern gradually decreases, so that hydrophilic area increases.The cuniform channel mentions
For La Pula pressure；By changing the hydrophobe area fraction inside cuniform channel, wetting gradient force is provided.The dyeing processing
Are as follows:
Liquor kalii iodide is paved on entire runner, is air-dried at room temperature, by the Portugal of the 100U/mg mixed with 1:1 volume ratio
The mixed solution of the horseradish peroxidase of grape carbohydrate oxidase and activity greater than 300unit/mg is paved on entire runner, room temperature
It air-dries.
Further, the functional layer patterning is divided into 10 segmentations.
Further, the runner outermost is hydrophobicity strip pattern structure, constitutes wedgy passage, prevents drop from spreading
To outside runner.
Further, the preparation method of the base chip: photoetching is carried out to a hydrophobic wedge bases, is constructed hydrophilic
Pattern.
A kind of examination of glucose concentration method based on the variation of color strip length, this method are based on described in claim 1
Chip realization, this method are as follows: glucose solution to be measured is dripped in runner initial end, is existed according to the glucose solution of various concentration
It is formed by the difference of color strip length in detection channel, can intuitively distinguish the size of concentration.
The present invention has the following advantages and beneficial effects:
(1) making apparatus needed for the present invention is simple, easy to operate, i.e. implementable in air；Mask plate is using printing skill
Art, cost of manufacture are cheap；
(2) the color strip length flowed on paper substrate micro-fluidic chip runner using the glucose solution of various concentration is different
This property, to detect glucose concentration, method is simple, easy to operate, and does not need complicated equipment.
Detailed description of the invention
Fig. 1 drop orients the schematic diagram sprawled in the gradient array and cuniform channel of strip.
Fig. 2 is the gradient array of strip and the structural schematic diagram of cuniform channel.
Fig. 3 is that the glucose solution of various various concentrations soaks the length of flow figure on surface in gradient.
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawing
Fig. 1,2 be base chip of the present invention structure chart, including cuniform channel, the key groove of cuniform channel is 4 °；Runner
Direction are as follows: from relatively narrow initial end to wider end；There is functional pattern, the functional pattern inside the cuniform channel
It is divided into multiple sectional areas along runner direction；If functional pattern in each sectional area by several band-like hydrophilic patterns and
The hydrophobic pattern alternative splicing of dry strip forms；The jackshaft of the hydrophobic pattern of the strip about cuniform channel or so pair
Claim, the hydrophobic pattern of the strip of each sectional area and the central axial direction of cuniform channel are parallel；From the beginning of cuniform channel
To end, the width of the hydrophobic pattern of strip is constant, and the spacing between two neighboring hydrophobic pattern is gradually increased, the hydrophobic figure of strip
Case gradually decreases, so that hydrophilic area increases.The cuniform channel provides La Pula pressure；By changing inside cuniform channel
Hydrophobe area fraction provides wetting gradient force.
We construct drop and orient the schematic diagram sprawled in the cuniform channel with stripe array gradient in Fig. 1,
The net axial laplace pressure gradient of middle liquid protrusion may be calculated:
α is the key groove of cuniform channel, and δ (x) is intrinsic channel width, and barometric gradient will drive drop to flow to channel more
Wide place.Show that laplace pressure gradient is directly proportional to α, with square being inversely proportional for δ (x).
In addition, gradient array, which is formed by Surface Energy Gradients equally, can provide a driving force Fd:
Initial contact radius when R is drop contact substrate, k are contact angle gradient of the drop along liquid drop movement direction, θ
For along the contact angle in liquid drop movement direction.
The resistance of liquid drop movement is equally the lag power F generated by contact angle hysteresish, expression formula are as follows:
Fh=2R γlv(cosθr-cosθa)
Wherein θrAnd θaIt is receding angle and advancing angle of the drop along wetting gradient direction.
By reasonable design surface soak gradient variation, can be good at control drop flow velocity and flowing away from
From.
In Fig. 2, w1 is cuniform channel start width, and L is runner total length,For cuniform channel angle, w0 is band
Width, l0 are the length of band, and p0 is the band center in first array of cuniform channel away from Δ p is band in adjacent array
Center away from variation be 0.04mm.By the structural parameters more than reasonably adjusting can change flow velocity of the drop in runner and
Length of flow, to adapt to different actual demands.
It is of the present invention it is hydrophilic refer to contact angle less than 5 °, on the contrary, it is hydrophobic refer to contact angle be greater than 150 °.
50 μ L liquor kalii iodides are paved on entire runner as shown in Figure 3, after being air-dried at room temperature 8min, by 0.8 μ L with
The horseradish peroxidase of glucose oxidase and activity greater than 300unit/mg of the 100U/mg of 1:1 volume ratio mixing mixes
It closes solution to be paved on entire runner, be air-dried at room temperature to get the paper substrate micro-fluidic core of examination of glucose concentration is used for the present invention
Piece.
In use, in runner section start at this moment one system then will be occurred for 2 μ L concentration glucose solution drop to be detected
The chemical reaction of column, glucose will be generated gluconic acid and hydrogen peroxide, subsequent hydrogen peroxide by glucose oxidase
Iodide are aoxidized to form brick-red iodine, at the same horseradish peroxidase also can by the hydrogen-peroxide reduction Cheng Shui of generation, then
The difference of color strip length is formed by detection channel according to the glucose solution of various concentration, can intuitively distinguish concentration
Size.
Embodiment 1
Method of the present invention using color strip length variation paper substrate micro-fluidic chip detection concentration of glucose, including
Following steps:
1) it prepares titanium dioxide nano-particle suspension: the titania powder that 0.1g particle size is 25nm or so is dissipated
In the ethyl alcohol of 10mL, ultrasound 1 hour makes it be uniformly dispersed.By the TMOS of 20 μ L (trimethoxyoctadecylsilane,
Octadecyl trimethoxysilane) being added to ultrasound in dispersion liquid, after ten minutes, reaction 12 hours, can be obtained silanization at room temperature
Titanium dioxide nano-particle suspension afterwards.
2) prepare the super-hydrophobic filter paper of micro-nano compound structure: filter paper be cut into the small pieces of 1.5cm × 3cm, then
Filter paper is immersed in the dispersion liquid that silane treatment is crossed, and is taken out after five minutes, is spontaneously dried at room temperature, repeatedly operation 3 times, i.e.,
It can obtain the super-hydrophobic filter paper with micro-nano compound structure.
3) it prepares mask plate: the wedge shape being pre-designed is printed on quartz glass by high-resolution laser printer
Pattern, wherein the parameter of pattern includes the width of wedge shaped pattern start width, pattern overall length, the angle of wedge of pattern, band, band
Length, band center in first array of cuniform channel away from band center in, adjacent array away from variation etc..Obtain strip
The quartz glass of gradient array and wedgy passage pattern is mask plate；The pattern is generally wedge-shaped, the transparent area
Entirety is divided into multiple regions, and the same area is uniformly distributed several about intermediate axial symmetry and to be parallel to the stripe-shape mask figure of vertical direction
Case, whole region outermost are angles at 4 ° and about two mask patterns of intermediate axial symmetry.The light-transparent pattern face in each region
Fraction is different, and pattern is from the initial end in relatively narrow region to end, and stripe-shape mask pattern is at gradient reduction appropriate, band-like light transmission
The area in region is gradually increased at gradient.
4) it prepares micro-fluidic chip: under the auxiliary of quartz mask plate, utilizing deep ultraviolet light UV/O3(275W, 390nm) is right
It carries out the exposure of selecting property of region option, and the silane of exposure area obtains hydrophilic runner by photodissociation, can will design on mask plate
Pattern copy on super-hydrophobic filter paper, to prepare the gradient array of strip and wetting gradient that wedgy passage is constituted
Self-driven type paper substrate micro-fluidic chip.
5) color development treatment of paper substrate micro-fluidic chip: 50 μ L liquor kalii iodides are paved on entire runner, to room temperature wind
After 8 minutes dry, glucose oxidase/horseradish peroxidase solution that 0.8 μ L is mixed with 1:1 volume ratio is paved with and is entirely being flowed
On road.A series of chemical reaction will occur when glucose solution drop is on runner, glucose will be by glucose oxidase
Oxidation is to generate gluconic acid and hydrogen peroxide, and subsequent hydrogen peroxide aoxidizes iodide to form brick-red iodine, while horseradish
Peroxidase also can be by the hydrogen-peroxide reduction Cheng Shui of generation.
6) color strip length-concentration of glucose java standard library is established.The glucose solution for taking known concentration draws 2 μ with dropper
L glucose solution drips on above-mentioned runner, a series of chemical reaction at this moment will occur, glucose will be glycoxidative by grape
To generate gluconic acid and hydrogen peroxide, subsequent hydrogen peroxide aoxidizes iodide to form brick-red iodine oxydasis, while peppery
Root peroxidase also can be by the hydrogen-peroxide reduction Cheng Shui of generation.Record the aqueous solution of the glucose corresponding face on runner
Colour band length.According to said method obtain in aqueous solution concentration of glucose corresponding color belt length when (be divided into 1%) from 1% to 99%
Degree.Obtain color belt length-corresponding java standard library of glucose concentration-.
7) it measures glucose concentration to be measured: drawing the grape to be measured of (2 μ L) equal with volume in java standard library with dropper
Sugar juice.Drop is on the runner of paper substrate micro-fluidic chip.Record color strip length of the glucose solution to be measured on runner.Then
It is compared with the color strip length-glucose concentration-java standard library in step 6, matches out the identical java standard library of color strip length
In glucose concentration, the concentration of glucose solution to be measured can be obtained.
The silane is the octadecyl front three oxosilane of 2 μ L, 97wt%.
The titanium dioxide partial size is 25nm.
The liquor kalii iodide is the liquor kalii iodide that 5 μ L purity are not less than 99.0%.
The glucose oxidase/horseradish peroxidase solution is the 100U/mg that 0.8 μ L is mixed with 1:1 volume ratio
Glucose oxidase and activity are greater than the horseradish peroxidase of 300unit/mg.
Embodiment 2
Draw 2.5mM, 5mM, 10mM, 15mM, 20mM, 25mM, 30mM, 40mM, 45mM, 50mM of 2 μ L respectively with dropper
Glucose solution, drip on the runner of paper substrate micro-fluidic chip.Obviously, different concentration of glucose solution corresponds to different length
The color belt of degree.The length that different glucose solution is flowed on runner can be readily measured with steel ruler.According to this side
Method can establish length of flow and the one-to-one java standard library of concentration of glucose, to detect the concentration of unknown glucose solution.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Range should not be construed as being limited to the specific forms stated in the embodiments, and protection scope of the present invention also includes art technology
Personnel conceive according to the present invention it is conceivable that equivalent technologies mean.
Claims (5)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304284A (en) * | 2020-03-06 | 2020-06-19 | 西南交通大学 | Method for rapidly detecting glucose by enzyme-polymer array coating microchip and application thereof |
| CN114182035A (en) * | 2021-11-17 | 2022-03-15 | 杭州孚斯泰生物技术有限公司 | Target nucleic acid instant detection method based on distance signal output |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040265171A1 (en) * | 2003-06-27 | 2004-12-30 | Pugia Michael J. | Method for uniform application of fluid into a reactive reagent area |
| US20060004267A1 (en) * | 2003-01-06 | 2006-01-05 | Peter Rule | Layered spectroscopic sample element with microporous membrane |
| CN101571538A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | Patterned biochemical analysis test paper and manufacturing method and application thereof |
| CN103105392A (en) * | 2013-01-20 | 2013-05-15 | 桂林理工大学 | Production method and application of patterned biochemical analysis test paper |
| CN103372469A (en) * | 2012-04-24 | 2013-10-30 | 中国科学院化学研究所 | Micropore thin film micro-fluidic chip as well as preparation method and application thereof |
| CN104931489A (en) * | 2015-05-17 | 2015-09-23 | 桂林理工大学 | Quantitative analysis method for measuring rapid test strip on basis of iodine-starch variable-color distance |
| CN105833814A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Method for manufacturing liquid drop self-driven microreactor and microreactor manufactured through method |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN105854964A (en) * | 2016-04-27 | 2016-08-17 | 浙江工业大学 | Micro-fluidic chip based on SERS detection, preparation method and application thereof |
| CN206082558U (en) * | 2016-04-27 | 2017-04-12 | 浙江工业大学 | Microfluid self -powered dynamic formula paper base micro -fluidic chip |
| CN107449927A (en) * | 2017-08-07 | 2017-12-08 | 厦门大学 | A kind of 3D integrates paper chip and visualization Quantitative detection target method |
| CN107632164A (en) * | 2017-09-18 | 2018-01-26 | 桂林理工大学 | A kind of inexpensive Ag+Detection method |
-
2019
- 2019-01-30 CN CN201910094129.6A patent/CN109839375A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060004267A1 (en) * | 2003-01-06 | 2006-01-05 | Peter Rule | Layered spectroscopic sample element with microporous membrane |
| US20040265171A1 (en) * | 2003-06-27 | 2004-12-30 | Pugia Michael J. | Method for uniform application of fluid into a reactive reagent area |
| CN101571538A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | Patterned biochemical analysis test paper and manufacturing method and application thereof |
| CN103372469A (en) * | 2012-04-24 | 2013-10-30 | 中国科学院化学研究所 | Micropore thin film micro-fluidic chip as well as preparation method and application thereof |
| CN103105392A (en) * | 2013-01-20 | 2013-05-15 | 桂林理工大学 | Production method and application of patterned biochemical analysis test paper |
| CN104931489A (en) * | 2015-05-17 | 2015-09-23 | 桂林理工大学 | Quantitative analysis method for measuring rapid test strip on basis of iodine-starch variable-color distance |
| CN105833814A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Method for manufacturing liquid drop self-driven microreactor and microreactor manufactured through method |
| CN105833926A (en) * | 2016-04-27 | 2016-08-10 | 浙江工业大学 | Microfluid self-driven paper base micro-fluidic chip and preparation method and application thereof |
| CN105854964A (en) * | 2016-04-27 | 2016-08-17 | 浙江工业大学 | Micro-fluidic chip based on SERS detection, preparation method and application thereof |
| CN206082558U (en) * | 2016-04-27 | 2017-04-12 | 浙江工业大学 | Microfluid self -powered dynamic formula paper base micro -fluidic chip |
| CN107449927A (en) * | 2017-08-07 | 2017-12-08 | 厦门大学 | A kind of 3D integrates paper chip and visualization Quantitative detection target method |
| CN107632164A (en) * | 2017-09-18 | 2018-01-26 | 桂林理工大学 | A kind of inexpensive Ag+Detection method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111304284A (en) * | 2020-03-06 | 2020-06-19 | 西南交通大学 | Method for rapidly detecting glucose by enzyme-polymer array coating microchip and application thereof |
| CN111304284B (en) * | 2020-03-06 | 2023-05-05 | 西南交通大学 | A method for rapid detection of glucose on an enzyme-polymer array coated microchip and its application |
| CN114182035A (en) * | 2021-11-17 | 2022-03-15 | 杭州孚斯泰生物技术有限公司 | Target nucleic acid instant detection method based on distance signal output |
| CN114182035B (en) * | 2021-11-17 | 2024-09-10 | 杭州孚斯泰生物技术有限公司 | Target nucleic acid instant detection method based on distance signal output |
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Application publication date: 20190604 |