CN109712862A

CN109712862A - Light path system suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument

Info

Publication number: CN109712862A
Application number: CN201910078983.3A
Authority: CN
Inventors: 宋家玉; 李康康; 刘文钊; 王晓龙
Original assignee: Autobio Labtec Instruments Zhengzhou Co Ltd
Current assignee: Autobio Experimental Instrument Zhengzhou Co Ltd; Autobio Labtec Instruments Zhengzhou Co Ltd
Priority date: 2019-01-28
Filing date: 2019-01-28
Publication date: 2019-05-03

Abstract

The invention discloses an optical path system suitable for a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, comprising an ion source chamber, a vacuum chamber, a mass analyzer, a detector, a laser, a laser position regulator, and a CCD imaging device; the CCD imaging device and the laser position adjuster are respectively arranged on the two sides of the mass analyzer on the outer wall of the ion source chamber; the laser reflector and the imaging reflector are respectively arranged on both sides of the ion flight tube in the ion source chamber through the angle adjustment bracket; The optical axis of the convex lens coincides with the optical axis of the laser focusing lens through the laser mirror to form a laser optical path; the optical axis of the filter of the CCD imaging device overlaps with the optical axis of the CCD imaging lens through the imaging mirror to form the CCD imaging optical path. The advantage of the invention lies in that the light emitted by the laser system is utilized to the maximum extent, and a high-definition image is formed to be presented on the operation interface, which is convenient for the user to observe the state of the sample target in real time, and the operation is convenient.

Description

Light path system suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument

Technical field

The present invention relates to Matrix-Assisted Laser Desorption Ionization Time of Flight instrument, swash more particularly, to suitable for Matrix-assisted The light path system of photodissociation analysis ionization time-of-flight mass spectrometer.

Background technique

Matrix-Assisted Laser Desorption Ionization Time of Flight instrument (MALDI-TOFMS；Hereinafter referred to as mass spectrograph) in need Sample target is bombarded with laser emission pulse laser.Existing laser system scheme is to generate pulsed laser ablation using laser Sample excitation goes out ion, and laser system and CCD imaging system are fixed on ion source cavity, passes through one standing wave of laser system fires Long light is transmitted on sample target through optical path, and proton obtains energy in a short time and is broken down into various fragment ions, due to respectively from The mass-to-charge ratio of son is different, and the movement velocity in tof tube (channel) is also different, the detector of flight tube terminal The ion flight map detected be also it is different, using judging the classification side of protein by analysis ion flight map Method come realize to differentiate object identification.CCD imaging system is observed under the cooperation of LED light source system by terminal presentation facility The case where sample target and position, currently, Laser emission optical path is tilted a certain angle respectively with CCD imaging optical path in ion source light path It is mounted on ion source cavity, angle is non-adjustable after installation is fixed, this allows for the position of Laser emission optical path and CCD imaging optical path The full machining accuracy by structural member and assembly precision are set to guarantee, therefore causes mass spectrometric consistency poor；Simultaneously as swashing Photosystem and CCD imaging system are that inclination is assemblied on ion source cavity, considerably increase the difficulty of installation and debugging.

Summary of the invention

It is an object of that present invention to provide a kind of optical path systems suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument System realizes that Matrix-Assisted Laser Desorption Ionization Time of Flight instrument is continuously adjusted laser irradiating position.

To achieve the above object, the present invention takes following technical proposals:

Light path system of the present invention suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument, including source housing, Vacuum chamber, mass analyzer, detector, laser, laser position adjuster, CCD imaging device；In the mass analyzer Ion flight pipe passes through the ion outlet position that the source housing extends to the vacuum chamber, is located at the ion outlet Ion flight pipe two sides are respectively arranged with laser focusing lens and CCD imaging lens；It is located at ion flight pipe in vacuum chamber Ion lens and sample target plate are sequentially arranged at intervals with immediately below import；The CCD imaging device and the laser position tune Section device is separately positioned on the two sides that source housing outer wall is located at mass analyzer；It is located at ion flight pipe two sides point in source housing Laser mirror and imaging mirror are not provided with by angle-adjusting bracket；The plano-convex lens optical axis of laser position adjuster is logical It crosses the laser mirror to coincide with the laser focusing lens optical axis, to constitute laser optical path；The filter of CCD imaging device Mating plate optical axis is coincided by imaging mirror and CCD imaging lens optical axis, to constitute CCD imaging optical path.

The CCD imaging device and the laser position adjuster are respectively perpendicular the down-set roof in source housing On.

The CCD imaging device and the laser position adjuster are horizontally set on the opposite two sidewalls of source housing respectively On.

The laser mirror is made of first laser reflecting mirror and second laser reflecting mirror, first, second laser Reflecting mirror is arranged in the source housing by angle-adjusting bracket respectively；The plano-convex lens light of the laser position adjuster Axis is coincided by the first, second laser mirror and the laser focusing lens optical axis, to constitute laser optical path.

The invention has the advantages that utilizing the light of laser system fires to greatest extent, the image for forming high-resolution is presented on behaviour Make on interface, it is easy to operate convenient for users to observing in real time sample target state.CCD imaging device and laser position are adjusted Device is respectively perpendicular down-set on the roof of source housing, or is horizontally set on the opposite two sidewalls of source housing, solves Existing be tilted a certain angle of having determined is mounted on deficiency present on ion source chamber body, meets Human Engineering Principle, significantly facilitates Actual installation and debugging, the user experience is improved to greatest extent.

Detailed description of the invention

Fig. 1 is structural schematic diagram of the invention.

Fig. 2 is CCD imaging device of the present invention and the laser position adjuster be respectively perpendicular it is down-set in ion Light path principle schematic diagram on source chamber roof.

Fig. 3 is that CCD imaging device of the present invention and the laser position adjuster are horizontally set on source housing respectively Schematic illustration in two lateral walls.

Specific embodiment

It elaborates with reference to the accompanying drawing to the embodiment of the present invention, the present embodiment before being with technical solution of the present invention It puts and is implemented, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to down State embodiment.

As shown in Figure 1, 2, the optical path system of the present invention suitable for Matrix-Assisted Laser Desorption Ionization Time of Flight instrument System, including by source housing 1, vacuum chamber 2, mass analyzer 3, detector 4, laser 5, laser position adjuster 6 and CCD at The mass spectrograph ontology formed as device 7；The ion flight pipe 8 of mass analyzer 3 passes through source housing 1 and extends to vacuum chamber 2 Ion outlet position, is located at that 8 arranged on left and right sides of ion flight pipe is respectively arranged with CCD imaging lens 9 and laser is poly- at ion outlet Focus lens 10；It is located at immediately below 8 import of ion flight pipe in vacuum chamber 2 and is sequentially arranged at intervals with ion lens 11 and sample target Plate 12；CCD imaging device 7 and laser position adjuster 6, which are respectively perpendicular, down-set is located at quality analysis in 1 roof of source housing The arranged on left and right sides of device 3.

It is located on the right side of ion flight pipe 8 in source housing 1 and is provided with first angle adjusting bracket and second angle adjusting branch Frame respectively corresponds on the first, second angle-adjusting bracket and is provided with first laser reflecting mirror 13 and second laser reflecting mirror 14, swashs The light that 15 optical axis of plano-convex lens of optical position adjuster 6 passes through the first, second laser mirror 13,14 and laser focusing lens 10 Axis coincides, to constitute laser optical path.

It is located on the left of ion flight pipe 8 in source housing 1 and is provided with third angle adjusting bracket and CCD optical path shading pipe 16, CCD imaging lens 9 are arranged in CCD optical path shading pipe 16, and imaging mirror 17 is provided in third angle adjusting bracket； 18 optical axis of optical filter of CCD imaging device 7 is coincided by the optical axis of imaging mirror 17 and CCD imaging lens 9, to constitute CCD imaging optical path.

As shown in figure 3, CCD imaging device 7 and laser position adjuster 6 can also be horizontally set on source housing 1 relatively Two sidewalls on.

Laser position adjuster 6 can use China Patent No. only for horizontal X Y-direction disclosed in ZL201620948406.7 The laser fiber head regulating device of adjusting is found to realize；Laser focusing lens 10 can use China Patent No. for Laser desorption ion source laser optical path disclosed in ZL201621168337.4 is realized.

The course of work of the present invention is summarized as follows:

Laser 5 is according to program instruction emission pulse laser, between laser wavelength 290nm-385nm, what laser 5 issued Light is transmitted in laser optical path by optical fiber 19.The pulsed light that laser 5 emits passes through plano-convex lens 15 for the pulsed light of input It is irradiated on first laser reflecting mirror 13 after carrying out collimation processing, reflects the light onto second laser on first laser reflecting mirror 13 On reflecting mirror 14, second laser reflecting mirror 14 is reflected the light onto again on laser focusing lens 10, is gathered light beam by focusing At sample on coke to sample target plate 12.Within the time of Laser emission, sample substrate moment obtains higher-energy and is divided Solution, centre bore under the action of ion lens 11 along ion lens 11 flies up upwards, by mass spectrograph ontology high voltage electric field come The flight of charged ion is controlled, charged ion is finally moved on detector 4 by the ion flight pipe 8 of mass analyzer 3, is examined It surveys device 4 and passes through the electronic signal detected, cooperate corresponding software library file, detectable substance is distinguished, reach testing goal.

For the ease of observing the situation of detection process and sample target spot, have with the design of the other side of laser optical path CCD imaging optical path, the laser 5 strike the fluorescence generated when sample substrate and are transmitted to imaging mirror by CCD imaging lens 9 On 17, which is conducted light to CCD by the reflection of imaging mirror 17 using a biconvex lens In the optical filter 18 of imaging device 7, light is imaged to operating software by circuit and software for CCD imaging device 7, realizes sample The real time monitoring of operation.The optical filter 18 of the sensitive film front end setting of CCD imaging device 7, it is 390nm- that effect, which is by wavelength, Light cut-off except 780nm wave band is fallen.The focus axis of laser optical path and CCD imaging optical path is on the surface of sample target plate 12.

In application debugging, it can realize that the position of input light is mobile by adjusting laser position adjuster 6；Pass through fine tuning First, second angle-adjusting bracket come adjust the first, second laser mirror 13,14 angle realize laser optical path incidence angle Degree is adjusted；By 17 angle of imaging mirror in fine tuning third angle adjusting bracket, realizes and observation position and angle are changed Become, preferably to observe.

In the description of the present invention, it should be noted that term "left", "right", " vertical ", "horizontal", "inner", "outside" The orientation or positional relationship of equal instructions is to be based on the orientation or positional relationship shown in the drawings, be merely for convenience of the description present invention and Simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with specific orientation construction And operation, therefore be not considered as limiting the invention.

Claims

1. An optical path system suitable for a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, comprising an ion source chamber, a vacuum chamber, a mass analyzer, a detector, a laser, a laser position regulator, and a CCD imaging device; the mass analyzer The ion flight tube extends through the ion source chamber to the ion outlet position of the vacuum chamber, and a laser focusing lens and a CCD imaging lens are respectively provided on both sides of the ion flight tube at the ion outlet; An ion lens and a sample target plate are arranged at intervals directly below the entrance of the ion flight tube; the CCD imaging device and the laser position regulator are respectively arranged on both sides of the mass analyzer on the outer wall of the ion source; it is characterized in that: The source chamber is provided with a laser mirror and an imaging mirror on both sides of the ion flight tube through angle adjustment brackets; the optical axis of the plano-convex lens of the laser position adjuster coincides with the optical axis of the laser focusing lens through the laser mirror, so that Constitute the laser light path; the optical axis of the filter of the CCD imaging device coincides with the optical axis of the CCD imaging lens through the imaging mirror, thereby forming the CCD imaging light path.

2. The optical path system suitable for matrix-assisted laser desorption ionization time-of-flight mass spectrometer according to claim 1, characterized in that: the CCD imaging device and the laser position regulator are respectively arranged vertically downward on the top of the ion source chamber on the wall.

3. The optical path system suitable for matrix-assisted laser desorption ionization time-of-flight mass spectrometer according to claim 1, wherein the CCD imaging device and the laser position adjuster are arranged horizontally on opposite sides of the ion source chamber respectively on the wall.

4. The optical path system suitable for matrix-assisted laser desorption ionization time-of-flight mass spectrometer according to claim 1, 2 or 3, wherein the laser mirror is composed of a first laser mirror and a second laser mirror, The first and second laser mirrors are respectively arranged in the ion source chamber through an angle adjustment bracket; the optical axis of the plano-convex lens of the laser position adjuster passes through the first and second laser mirrors and the laser focusing lens light The axes coincide to form the laser light path.