CN109699537B

CN109699537B - Breeding method of stichopus japonicus artificial seedlings

Info

Publication number: CN109699537B
Application number: CN201810027951.6A
Authority: CN
Inventors: 冯永勤
Original assignee: Hainan University
Current assignee: Hainan University
Priority date: 2018-01-11
Filing date: 2018-01-11
Publication date: 2021-11-26
Anticipated expiration: 2038-01-11
Also published as: CN109699537A

Abstract

The invention provides a breeding method of stichopus japonicus artificial offspring seeds, which comprises the steps of parent stichopus japonicus cultivation, artificial stimulation egg collection, fertilized egg incubation, larva cultivation, seedling collection, young stichopus japonicus cultivation and the like. The method explores the optimal time and method for artificially stimulating and collecting the stichopus japonicus selenka roe and the cultivation method of the ear-shaped larva, the young stichopus japonicus selenka and the young stichopus japonicus selenka, optimizes the collecting tool in the pond-dumping process of the young stichopus japonicus selenka and the young stichopus japonicus selenka, and obviously improves the survival rate of the young stichopus japonicus selenka and the young stichopus japonicus selenka.

Description

Breeding method of stichopus japonicus artificial seedlings

Technical Field

The invention belongs to the field of aquaculture, and particularly relates to a breeding method of stichopus japonicus artificial seedlings.

Background

Stichopus japonicus selenka, school name: stichopus variegatus is a marine animal of Apostichopus genus of Apostichopus family, commonly known as "yellow meat" and "square ginseng", is thick and tender in meat quality, and is a high-quality edible sea cucumber. The protein content of the stichopus japonicus selenka is 73.81 percent, the fat content is 1.51 percent, the total amount of amino acid is 66.46 percent, the unsaturated fatty acid content is 39.25 percent, and the polyunsaturated fatty acid content is 5.56 percent. Has the characteristics of high protein and low fat, has very high nutritional value, health care function and economic value, and the product is not in demand in the domestic market. In the world, stichopus japonicus is also an important edible tropical sea cucumber variety and a top-grade aquatic product for earning foreign exchange in export. Geographic distribution: taiwan, Guangxi 28064, Zhou island, Guangdong island of Salmo, Hainan island and Xisha island; the west has distribution from madagascar, sang-barr and red sea, eastern to galolin archipels, north to southern japan, south to lode-hao islands in australia.

However, according to the current Stichopus japonicus fry breeding technical research, a second report about the successful breeding of Stichopus japonicus fry is not seen in China except the news report of the inventor. There are two main problems: firstly, the success rate of egg collection by stimulation of the parent ginseng is low, and a large amount of fertilized eggs are difficult to obtain; secondly, the percentage of 5 pairs of spheroids appearing in the stage of the big ear-shaped larva is low, and the percentage of the grown metamorphosis to the goblet-shaped larva is low; thirdly, the phenomena of ginseng body atrophy, skin ulceration and autolysis are easy to occur to the young stichopus japonicus, and the survival rate of the young stichopus japonicus is low.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provide the breeding method of the stichopus japonicus artificial offspring seeds, which has the advantages of high success rate of stimulating egg collection, high survival rate of young stichopus japonicus cultivation and high-quality stichopus japonicus offspring seeds cultivated.

The invention is realized by the following steps:

a breeding method of stichopus japonicus artificial seedlings is characterized by comprising the following steps:

(1) parent ginseng cultivation:

catching natural flower stichopus japonicus, selecting a person with a plump and non-injured ginseng body as a parent stichopus japonicus, breeding in an indoor cement pond or an outdoor pond, and placing a concealed object at the bottom of the pond; taking sargassum thunbergii powder, gulfweed powder, stichopus japonicus parent sea compound feed and sea mud as baits, and feeding for 1 time every evening, wherein the feeding amount is 2-3% of the weight of the parent sea cucumbers; the indoor cement pond culture keeps continuous flowing water and aeration, and residual bait and excrement at the bottom of the pond are periodically removed; changing water for 1 time every other day in the outdoor pond;

(2) artificial stimulation egg taking:

in the egg laying season of stichopus japonicus, artificial stimulation egg collection is carried out within 3 days before and after the first one of each month of the lunar calendar.

(3) Hatching fertilized eggs:

after the sperm and eggs are discharged from the parent sea cucumber, fishing out the parent sea cucumber by using a small dip net made of nylon thread, siphoning and changing water by using a 300-mesh silk net cage for 1-2 times, wherein the water change amount is 50-70% each time, and hatching fertilized eggs in an original stimulation egg taking pond;

(4) larva cultivation:

after the fertilized eggs are hatched and developed into primary ear larvae, collecting the primary ear larvae, and then putting the primary ear larvae into a seedling pond for cultivation; feeding Chaetoceros muelleri, Isochrysis galbana, Spirulina powder and yeast for 2 times per day; adding sand-filtered seawater of 9-11% of the water body for seedling culture every day from the 1 st to the 3 rd days of larva stocking, and changing water by 20-30% every day after the 4 th day;

(5) seedling picking:

when 10-20% of big ear larvae are transformed into goblet-shaped larvae, putting polyethylene corrugated plate seedlings for collection; putting corrugated plates, and feeding 2 times per day with flagellates such as Chaetoceros muelleri, Strongylocentrotus globulus, Spirulina powder, yeast and Bacillus as bait; stopping changing water during seedling picking, and starting flowing water to improve water quality after the goblet-shaped larvae are attached;

(6) culturing young sea cucumbers:

in 10 days after the attachment and metamorphosis of the goblet-shaped larva is young sea cucumber, except for feeding spirulina, yeast and bacillus, young sea cucumber compound feed, sargassum thunbergii powder, sargassum powder and sea mud are fed for 1 time respectively in the morning and evening, and the young sea cucumber is cultivated under the conditions of micro-flowing water and continuous aeration; stopping adding spirulina and yeast on days 11-40 after young sea cucumber is attached, and adding for 1 time in the morning and evening respectively; culturing under the conditions of micro-flowing water and continuous aeration; removing residual bait and feces at the bottom of the pond 1 time every 5-7 days, collecting young sea cucumbers by adopting a 40-mesh silk net cage, and timely transferring the collected young sea cucumbers into a seedling pond; when the young stichopus japonicus grows to reach 1.0cm, starting to enter a young stichopus japonicus cultivation stage;

(7) cultivating young sea cucumbers:

feeding young sea cucumber compound feed, sargassum thunbergii powder, gulfweed powder, sea mud and bacillus for 1 time respectively in the morning and evening; culturing under the conditions of micro-flowing water and continuous aeration; removing residual bait and feces at the bottom of the pond for 1 time every 3-5 days, and collecting young ginseng by using a 20-mesh silk net cage; the culture was carried out for about 40 days.

Preferably, step (1) is: selecting 400-850g natural flower stichopus japonicus, stocking in an indoor cement pond or an outdoor pond for cultivation, wherein the stocking density of the indoor pond is 5-8 heads/m²Outdoor pond stocking density is 1-2 heads/m²The bottom of the pool is provided with a concealed object; feeding 1 time in the evening every day, wherein the daily feeding amount is 2-3% of the weight of the parent ginseng, and the feeding bait for the parent ginseng comprises 22-27 parts by weight of sargassum thunbergii powder, 22-27 parts by weight of gulfweed powder, 38-42 parts by weight of stichopus japonicus parent ginseng compound feed and 8-12 parts by weight of sea mud; the indoor cement pond culture keeps continuous flowing water and aeration, the daily flow rate is 2-4 times of the culture water body, and residual baits and feces at the bottom of the pond are periodically removed; changing water for 1 time every other day in the outdoor pond, wherein the daily water change amount is 10-20% of the water body of the pond; the salinity of the seawater is 26-33, and the dissolved oxygen is more than 5 mg/L.

Further preferably, in the step (1), the bait for feeding the parent sea cucumber comprises 25 parts by weight of sargassum thunbergii powder, 25 parts by weight of sargassum powder, 40 parts by weight of stichopus japonicus parent sea cucumber compound feed and 10 parts by weight of sea mud.

Preferably, step (2) is: in the spawning season of stichopus japonicus in 5-9 months, selecting to induce spawning within 3 days before and after the first lunar calendar of each month, stopping feeding 2 days before artificial stimulation spawn collection, selecting healthy parent stichopus japonicus with plump abdomen in the afternoon of the day of stimulation spawn collection, washing the surfaces of the ginseng bodies with filtered seawater, then placing the ginseng bodies in an indoor seedling raising pond for exposing to the air and drying in the shade for 40-60min, then stimulating with filtered seawater flowing water for 30-40min, then injecting seawater, and waiting for sperm and spawn to discharge;

preferably, step (3) is: after the sperm egg is discharged, fishing out the parent sea cucumber by adopting a nylon wire hand-made net, siphoning to remove excrement, then manufacturing a water exchanger by adopting a 300-mesh silk net, changing water for 1-2 times, wherein the water changing amount is 50-70% each time, and hatching the fertilized egg in the original stimulation egg taking pool.

Preferably, step (4) is: after the fertilized eggs are hatched and developed into primary ear larvae, collecting the primary ear larvae by adopting a siphon method, and then putting the primary ear larvae into a seedling pond for cultivation, wherein the stocking density of the primary ear larvae is 0.1-0.3 per mL; using Chaetoceros muelleri, Isochrysis galbana, spirulina powder and yeast as bait, throwing bait for 2 times every day, each time throwing Chaetoceros muelleri cell 1-2 ten thousand cells/mL, Isochrysis galbana cell fine-breaking 1000 cells/mL 2000 cells/mL, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L, bacillus 0.05-0.10 mg/L; adding sand-filtered seawater of 9-11% of the water body for seedling culture every day from the 1 st to the 3 rd days of larva stocking, and changing water by 20-30% every day after the 4 th day; culturing and developing the larva for 10-14 days to obtain goblet-shaped larva;

further preferably, in the step (4), 10% sand-filtered seawater of the nursery water body is added every day from the 1 st to the 3 rd days of the larva stocking.

Preferably, step (5) is: when 10-20% of the big ear larva is transformed into a goblet-shaped larva, 100 corrugated plates and 120 corrugated plates with the specification of 40cm multiplied by 30cm are put in each square meter. After the corrugated plate is put in until the goblet-shaped larvae are attached, 2 times of bait feeding are carried out every day, 2 ten thousand cells/mL of Chaetoceros muelleri cells, 2000 cells/mL of Isochrysis galbana, 0.12mg/L of spirulina powder, 0.12mg/L of yeast and 0.10mg/L of bacillus are put in each time; and stopping changing water during seedling picking, and starting flowing water to improve the water quality after the goblet-shaped larvae are attached.

Preferably, the first and second electrodes are formed of a metal,the step (6) is as follows: within 10 days after the attachment and metamorphosis of the goblet-shaped larva is young sea cucumber, the young sea cucumber compound feed, the sargassum thunbergii powder, the sargassum powder and the sea mud are fed in addition to the spirulina, the yeast and the bacillus, and the feeding amount is 0.4 to 0.6g/m of the spirulina powder each time²0.1-1.2g/m yeast²Bacillus 0.1-1.2g/m²0.3-2.3g/m of young sea cucumber compound feed²Sargassum thunbergii powder 0.3-2.3g/m²Sargassum powder 0.3-2.3g/m²Sea mud 1.0-3.0g/m²1 time in the morning and evening; culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flow is 0.5-1.0 time of the water body for seedling culture; stopping feeding spirulina and yeast from 11 th to 40 th days after the young stichopus japonicus is attached, wherein the feeding concentration is 2.5-5.0g/m of the young stichopus japonicus compound feed each time²2.5-5.0g/m of sargassum thunbergii powder²2.5-5.0g/m of Sargassum powder²Sea mud 3.5-5.0g/m²Bacillus 0.2-1.0g/m²The administration is given 1 time in the morning and evening respectively. Culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flowing water is 1.5-2.0 times of the water body for seedling culture, and the dissolved oxygen is kept above 5 mg/L. Removing residual bait and feces at the bottom of the pond for 1 time every 5-7 days; when the young sea cucumber grows to reach 1.0cm of body length, the young sea cucumber starts to enter a young sea cucumber cultivation stage.

Preferably, step (7) is: the growth length of the young sea cucumber seedlings reaches more than 1cm about 40 days after the attachment and metamorphosis of the goblet-shaped larvae is the young sea cucumber, and the number of the young sea cucumber cultured per square meter is 0.2-0.5 ten thousand at the stage that the young sea cucumber body is 1-8 cm. The concentration of the bait is 5.5-8.0g/m of young sea cucumber compound feed²5.5-8.0g/m of sargassum thunbergii powder²5.5-8.0g/m of Sargassum powder²5.5-10.0g/m of sea mud²Bacillus 1.0-1.2g/m². The administration is given 1 time in the morning and evening respectively. Culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flowing water is 1.0-2.0 times of the water body for seedling culture, and the dissolved oxygen is kept above 5 mg/L. Removing residual bait and feces at the bottom of the pond for 1 time every 3-5 days; when the bottom of the pond is washed, the washed young sea cucumbers are collected by a silk net box with a 20-mesh sieve and are timely moved back to the seedling raising pond, so that the young sea cucumbers are prevented from overstocking, being injured and dying.

The invention explores the optimal opportunity and the induction method for inducing the stichopus japonicus to spawn and the cultivation method for the ear-shaped larvae, the young stichopus japonicus and the young stichopus japonicus, optimizes the collecting tool in the process of pouring the young stichopus japonicus and the young stichopus japonicus into the pond, and obviously improves the survival rate of the young stichopus japonicus and the young stichopus japonicus.

Detailed Description

The invention will be further understood by reference to the following examples.

Example 1

(1) Parent ginseng cultivation:

selecting 400-850g natural flower stichopus japonicus, stocking in an indoor cement pond or an outdoor pond for cultivation, wherein the stocking density of the indoor pond is 5-8 heads/m²Outdoor pond stocking density is 1-2 heads/m²The bottom of the pool is provided with a concealed object; feeding 1 time every evening, wherein the daily feeding amount is 2-5% of the weight of the parent ginseng, and the feeding baits for the parent ginseng comprise sargassum thunbergii powder, gulfweed powder, stichopus japonicus parent ginseng compound feed, sea mud and the like in parts by weight, and respectively account for 25%, 40% and 10% of the feeding amount. The indoor cement pond culture keeps continuous flowing water and aeration, the daily flow rate is 2-4 times of the culture water body, and residual baits and feces at the bottom of the pond are periodically removed; the water change of the outdoor pond is carried out for 1 time every other day, and the daily water change amount is 10-20% of the water body of the pond. The salinity of the seawater is 26-33, and the dissolved oxygen is more than 5 mg/L.

(2) Artificial stimulation egg taking:

in the spawning season of stichopus japonicus in 5-9 months, artificial stimulation spawn collection is carried out within 3 days before and after the first lunar calendar of each month, bait feeding is stopped 2 days before stimulation spawn collection, healthy parent stichopus japonicus with plump abdomen is selected in the afternoon of the day of spawning induction, the surfaces of the stichopus japonicus are washed clean by filtered seawater, then the stichopus japonicus is placed in an indoor seedling raising pond to be exposed and dried in the air for 60min, then the stichopus japonicus is stimulated by filtered seawater flowing water for 30min, then seawater is injected, and the sperm and spawn are discharged. The success rate of artificial stimulation egg taking reaches 100 percent.

(3) Hatching fertilized eggs:

after the sperm egg is discharged, fishing out the parent sea cucumber, siphoning to remove excrement, adopting a 300-mesh silk net to manufacture a water exchanger, changing water for 1-2 times, wherein the water changing amount is 70% each time, and hatching the fertilized egg in an original induced spawning pond.

(4) Larva cultivation:

after the fertilized eggs are hatched and developed into primary ear larvae, collecting the primary ear larvae by a siphon method, and then putting the primary ear larvae into a seedling pond for cultivation, wherein the stocking density of the primary ear larvae is 0.1-0.3 per mL. Using Chaetoceros muelleri, Isochrysis galbana, spirulina powder and yeast as bait, throwing bait for 2 times every day, each time throwing Chaetoceros muelleri cell 1-2 ten thousand cells/mL, Isochrysis galbana cell fine-breaking 1000 cells/mL 2000 cells/mL, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L, bacillus 0.05-0.10 mg/L; 3, during the 1 st to 3 rd days of larva stocking, adding sand-filtered seawater of 10 percent of seedling-raising water body every day, and changing water by 20-30 percent every day after 4 th day; the larva is cultivated, developed and transformed into a goblet-shaped larva after 10-14 days. The seedling rate of the young ear is 1.5-2.5%.

(5) Seedling picking:

when 10-20% of the big ear larva is transformed into a goblet-shaped larva, 100 corrugated plates and 120 corrugated plates with the specification of 40cm multiplied by 30cm are put in each square meter. After the corrugated plate is put in the vessel-shaped larva, 2 times of bait feeding are carried out every day, 2 ten thousand cells/mL of Chaetoceros muelleri cells, 2000 cells/mL of Isochrysis galbana, 0.12mg/L of spirulina powder, 0.12mg/L of yeast and 0.10mg/L of bacillus are fed in each time. And stopping changing water during seedling picking, and starting flowing water to improve the water quality after the goblet-shaped larvae are attached.

(6) Culturing young sea cucumbers:

within 10 days after the attachment and metamorphosis of the goblet-shaped larva is young sea cucumber, the young sea cucumber compound feed, the sargassum thunbergii powder, the sargassum powder and the sea mud are fed in addition to the spirulina, the yeast and the bacillus, and the feeding amount is 0.4 to 0.6g/m of the spirulina powder each time²0.1-1.2g/m yeast²Bacillus 0.1-1.2g/m²0.3-2.3g/m of young sea cucumber compound feed²Sargassum thunbergii powder 0.3-2.3g/m²Sargassum powder 0.3-2.3g/m²Sea mud 1.0-3.0g/m²1 time in the morning and evening; culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flow is 0.5-1.0 time of the water body for seedling culture,

stopping feeding spirulina and yeast from 11 th to 40 th days after the young stichopus japonicus is attached, wherein the feeding concentration is 0.2-1.0g/m for each time²2.5-5.0g/m of young sea cucumber compound feed²2.5-5.0g/m of sargassum thunbergii powder²2.5-5.0g/m of Sargassum powder²Sea mud 3.5-5.0g/m²The administration is given 1 time in the morning and evening respectively. Culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flowing water is 1.5-2.0 times of the water body for seedling culture, and the dissolved oxygen is kept above 5 mg/L. Removing residual bait and feces at the bottom of the pond for 1 time every 5-7 days; when the young sea cucumber grows to reach 1.0cm of body length, the young sea cucumber starts to enter a young sea cucumber cultivation stage.

(7) Cultivating young sea cucumbers:

the concentration of the bait is 5.5-8.0g/m of young sea cucumber compound feed²5.5-8.0g/m of sargassum thunbergii powder²5.5-8.0g/m of Sargassum powder²5.5-10.0g/m of sea mud²Bacillus 1.0-1.2g/m²The administration is given 1 time in the morning and evening respectively. Culturing under the conditions of micro-flowing water and continuous aeration, wherein the daily flowing water is 1.0-2.0 times of the water body for seedling culture, and the dissolved oxygen is kept above 5 mg/L. The residual baits and the feces at the bottom of the pond are removed for 1 time every 3 to 5 days. About 40 days after the attachment of the goblet-shaped larvae, the growth length of the young ginseng seedlings reaches more than 1cm, the number of the young ginseng cultivated per square meter is 0.2-1.0 ten thousand at the stage that the young ginseng bodies are 1-8cm long, wherein the length of the young ginseng cultivated in the indoor seedling raising pool of one square meter is about 2000.

The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.

Claims

1. a breeding method for artificial seed of Apostichopus japonicus, is characterized in that, comprises the following steps:

(1) Parent ginseng cultivation:

Harvest natural sea cucumbers, select full-bodied and uninjured individuals as parent ginseng, stock them in indoor cement ponds or outdoor ponds, and place hidden objects at the bottom of the pool; Compound feed and sea mud are used as bait, and the bait is fed once every evening, and the feeding amount is 2-3% of the body weight of the ginseng; the indoor cement pond is cultivated to maintain continuous water flow and aeration, and the residual bait and feces at the bottom of the pond are regularly removed; the outdoor pond is separated Change the water once a day;

(2) Artificially stimulated egg collection:

In the spawning season of A. japonicus from May to September, choose to induce spawning within 3 days before and after the first day of the lunar calendar each month, stop feeding 2 days before artificial stimulation of egg collection, and select the abdomen in the afternoon of the day of stimulation for egg collection. For plump and healthy ginseng, rinse the surface of the ginseng with filtered seawater, then put it in the indoor nursery pond to dry in the shade for 40-60min, then stimulate it with filtered seawater running water for 30-40min, and then inject seawater until the sperm and eggs are discharged;

(3) The fertilized egg hatches:

After the sperm and eggs are discharged from the ginseng, the ginseng is taken out with a cheat net made of nylon thread, and the feces are removed by siphoning; the water is changed by siphoning with a 300-mesh silk net box, and the water is changed 1-2 times, and the amount of water is 50-70% each time. , the fertilized eggs hatch in the original stimulated egg collection pool;

(4) Nurturing of larvae:

After the fertilized eggs hatched and developed into larvae, the larvae were collected by the siphon method, and then the larvae were placed in the nursery tank for cultivation. The stocking density of the larvae was 0.1-0.3/mL; Algae, spirulina powder and yeast are used as bait, feeding twice a day, 1-20,000 cells/mL of Chaetoceros muzieri each time, 1000-2000 cells/mL of Isochrysis globosa, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L, Bacillus 0.05-0.10mg/L; from the 1st to the 3rd day of larvae stocking, add 9-11% sand filter seawater of the nursery water every day, after the 4th day Change the water 20-30% every day; after 10-14 days of cultivation, the larvae metamorphose into bottle-shaped larvae;

(5) Picking seedlings:

When 10-20% of the big-ear larvae have metamorphosed into bottle-shaped larvae, put corrugated board to pick seedlings, and put 100-120 pieces of corrugated board with a size of 40cm × 30cm per square meter; 2 times, 20,000 cells/mL of Chaetoceros muffini, 2,000 cells/mL of Isochrysis globosa, 0.12mg/L of spirulina powder, 0.12mg/L of yeast, 0.10mg/L of Bacillus; seedling collection Stop changing the water during the period, and after the bottle-shaped larvae are attached, start running water to improve the water quality;

(6) Cultivation of young ginseng:

Within 10 days after the bottle-shaped juveniles attach and metamorphose to juvenile ginseng, in addition to feeding spirulina, yeast and bacillus, start feeding juvenile ginseng compound feed, salina powder, sargassum powder and sea mud, in the morning and evening. 1 injection, cultivated under micro-flow water and continuous aeration conditions; from the 11th to 40th day after the attachment of juvenile ginseng, stop the injection of spirulina and yeast, and once in the morning and evening; cultivated under micro-flow water and continuous aeration conditions; every interval Remove the residual bait and feces at the bottom of the pool once every 5-7 days, collect the juvenile ginseng with a 40-mesh sieve cage, and transfer the collected juvenile ginseng to the nursery tank in time; when the juvenile ginseng grows to a body length of 1.0cm, start Enter the stage of young ginseng cultivation;

(7) Cultivation of young ginseng:

Use the compound feed of young ginseng, Sargassum powder, Sargassum powder, sea mud and Bacillus as bait, once in the morning and once in the evening; cultivate under the condition of micro-flow water and continuous aeration; remove the residues at the bottom of the pool every 3-5 days The bait and feces were used once, and the young ginseng was collected in a 20-mesh silk net box; cultured for 40 days; the specific operation was: 40 days after the bottle-shaped larvae were attached to the metamorphic juvenile ginseng, the ginseng seedlings grew to a length of more than 1 cm, and when the young ginseng grew In the stage of 1-8cm, the amount of juveniles per square meter is 0.2-0.5 million; the concentration of each feeding is 5.5-8.0g/m ² of juvenile ginseng compound feed, 5.5-8.0g/m ² of Sargassum powder, horsetail Algal powder 5.5-8.0g/m ² , sea mud 5.5-10.0g/m ² , Bacillus 1.0-1.2g/m ² ; once in the morning and once in the evening; cultivated under micro-flow water and continuous aeration conditions, the daily flow of water It is 1.0-2.0 times of the water body for seedlings, and the dissolved oxygen is kept above 5mg/L; the residual bait and feces at the bottom of the pool are removed every 3-5 days; , and promptly moved back to the nursery pond to avoid backlog of young ginseng injury and death.

2. the breeding method of the artificial seed of Apostichopus japonicus according to claim 1, is characterized in that, step (1) is: select natural sea cucumber of body weight 400-850g, stocking in indoor cement pond or outdoor pond and cultivate, The stocking density of indoor pools is 5-8 heads/m ² , and the stocking density of outdoor ponds is 1-2 heads/m ² , and shelters are placed at the bottom of the pools; bait is fed once a day in the evening, and the daily feeding amount is 2-3% of the body weight of the parent ginseng , wherein, according to parts by weight, the feeding bait for ginseng includes 22-27 parts of sage powder, 22-27 parts of sargassum powder, 38-42 parts of compound feed of sea cucumber and ginseng and 8-12 parts of sea mud; The indoor cement pond is cultivated to maintain continuous water flow and aeration. The daily water flow is 2-4 times that of the cultivated water body, and the residual bait and feces at the bottom of the pool are regularly removed; the outdoor pond is changed every other day, and the daily water change is 10-20% of the pond water body. ; The salinity of seawater is 26-33, and the dissolved oxygen content is above 5mg/L.

3. The breeding method of artificial seedlings of Apostichopus japonicus according to claim 2, characterized in that, in step (1), according to the parts by weight, the feeding bait of the parent ginseng comprises 25 parts of Sargassum powder, horsetail 25 parts of algae powder, 40 parts of Apostichopus japonicus parent and ginseng compound feed and 10 parts of sea mud.

4. The breeding method of the artificial seed of Apostichopus japonicus according to claim 1, characterized in that, step (6) is: within 10 days after the juveniles are attached and metamorphosed into juvenile ginseng, except for feeding spirulina, yeast and In addition to Bacillus, start to feed juvenile ginseng compound feed, Salina powder, Sargassum powder and sea mud, each feeding amount is Spirulina powder 0.4-0.6g/m ² , Yeast 0.1-1.2g/m ² , Bacillus 0.1-1.2g/m ² , Juvenile ginseng compound feed 0.3-2.3g/m ² , Salina powder 0.3-2.3g/m ² , Sargassum powder 0.3-2.3g/m ² , Sea mud 1.0- 3.0g/m ² , once in the morning and once in the evening; cultivated under the conditions of micro-flow water and continuous aeration, the daily water flow is 0.5-1.0 times that of the seedling water body; from the 11th to the 40th day after the juvenile ginseng is attached, stop the injection of spirulina and Yeast, each feeding concentration is juvenile ginseng compound feed 2.5-5.0 g/m ² , Salina powder 2.5-5.0 g/m ² , Sargassum powder 2.5-5.0 g/m ² , sea mud 3.5-5.0 g/ m ² , Bacillus 0.2-1.0g/m ² , once in the morning and once in the evening; cultivated under the conditions of micro-flow water and continuous aeration, the daily flow of water is 1.5-2.0 times that of the seedling water body, and the dissolved oxygen is kept above 5mg/L; Remove the residual bait and feces at the bottom of the pool once every 5-7 days; when the juvenile ginseng grows to a body length of 1.0 cm, it begins to enter the young ginseng cultivation stage.