CN109679818A

CN109679818A - A kind of crocodile bone and flesh health liquor and preparation method thereof

Info

Publication number: CN109679818A
Application number: CN201910016311.XA
Authority: CN
Inventors: 洪鹏志; 周春霞; 邓楚津; 刘唤明; 杨萍
Original assignee: Guangdong Ocean University
Current assignee: Guangdong Ocean University
Priority date: 2019-01-08
Filing date: 2019-01-08
Publication date: 2019-04-26

Abstract

The invention discloses a kind of crocodile bone and flesh health liquors, including components such as crocodile meat enzymolysis liquid, crocodile bone meal and white wine, the invention also discloses a kind of preparation methods of crocodile bone and flesh health liquor, alligator bone is made ultra micro bone meal, crocodile meat is made to crocodile meat enzymolysis liquid by the present invention, crocodile bone meal and crocodile meat enzymolysis liquid are put into white wine and are impregnated, crocodile bone and flesh health liquor is made, the nutritional ingredient of crocodile bone and flesh is set to have obtained filling benefit extraction and utilize lipoprotein, a variety of amino acid, the vitamin etc. for being rich in animal, nutritive value is very high.The Chinese medicines such as jujube, lycium ruthenicum, American Ginseng are also added into the present invention, not only entrance is fine and smooth, and without Chinese medicine bitter taste, in good taste, long-term use has the health-care efficacy of tonifying kidney and strengthening yang, life essence-filling, marrow-benefitting, nourishing liver and kidney, blood-supplementing blood-nourishing, nourishing the blood and yin, blood-nourishing brain tonic.In addition preparation method of the invention, process is simple, reduces the loss of material nutrient component, improves the utilization efficiency of raw material, is appropriate for producing in batches, be worthy to be popularized.

Description

A kind of crocodile bone and flesh health liquor and preparation method thereof

Technical field

The present invention relates to health liquor and its preparation technical field, specifically a kind of crocodile bone and flesh health liquor and its preparation side Method.

Background technique

Although the initial drinking behavior of the mankind can't be referred to as health of drinking, there is close connection with health care System.Initial wine is made of the wild fruit of mankind's acquisition obtains suitable condition spontaneous fermentation when residue, due to being permitted More wild fruits inherently have medical value, so initial wine can deserve to be called natural " health liquor ", it is to human body Health has certain protection and facilitation.With the improvement of living standards, demand of the people to health is also higher and higher, pursue The mode of health is also more and more.The health liquor noun completely new as one, just gradually comes into people's lives.

Existing various health liquors mostly use immersion way, this makes after the animal meat impregnated in its hetero-organization Bioactive substance cannot thoroughly be dissolved in wine, and in animal meat bioactive substance only dissolves again in rice wine It can be just released bioactive molecule, so general health liquor lacks nutritional ingredient and health-care effect, also do not have tallow Fragrant mouthfeel.

Summary of the invention

The purpose of the present invention is to provide a kind of crocodile bone and flesh health liquors and preparation method thereof, to solve above-mentioned background technique The problem of middle proposition.

To achieve the above object, the invention provides the following technical scheme:

A kind of crocodile bone and flesh health liquor, including following component by weight: 120-150 parts of crocodile meat enzymolysis liquid, crocodile 150-180 portions of fishbone dust, 40-60 parts of lycium ruthenicum, 20-30 parts of green tea powder, is modified Gorgon fruit 15-25 parts, covers basin 50-70 parts of jujube 10-15 parts sub-, 8-13 parts of Morinda officinalis, 8-12 parts of radix glycyrrhizae preparata, 7-12 parts of American Ginseng, 6-10 parts of dark plum, 5-8 parts of Radix Astragali, radix cynanchi bungei 3-5 parts of extract, 4500-5000 parts of white wine.

A kind of preparation method of crocodile bone and flesh health liquor, specifically includes the following steps:

S1, preparation crocodile meat enzymolysis liquid: fresh crocodile meat is taken, the preparation of crocodile meat enzymolysis liquid is carried out；

S2, preparation crocodile bone meal: fresh alligator bone is taken, crocodile bone meal is prepared；

S3, preparation green tea powder: green tea fresh, high-quality, without rotten leaf is taken, the preparation of green tea powder is carried out；

S4, it prepares modified Gorgon fruit: taking appropriate Gorgon fruit, be modified the preparation of Gorgon fruit；

S5, it prepares bunge auriculate root extract: selecting high-quality complete, the radix cynanchi bungei root tuber without mildew, carry out bunge auriculate root extract Preparation；

S6, other pretreatments of raw material: the raw material of other components are pre-processed to obtain mixed powder；

S7, an immersion treatment: taking white wine, crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder by weight, by white wine, Crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder are placed in wine jar, sealed soak 30d, and soaking temperature is 20-24 DEG C, are produced Object A；

S8, secondary immersion treatment: taking modified Gorgon fruit, bunge auriculate root extract by weight, is added in product A, is added simultaneously The mixed powder that step S6 is obtained continues to impregnate 45d, and soaking temperature is 18-22 DEG C, obtains product B；

S9, ultracentrifugation processing: product B is placed in Ultracentrifuge, ultracentrifugation is carried out, takes supernatant i.e. Obtain semi-finished product health liquor；

S10, filling process: it is filling to the progress of semi-finished product health liquor using aseptic filling technology, bottle is carried out after filling Pasteurize obtains crocodile bone and flesh health liquor.

It is further: in the step S1, to prepare the detailed process of crocodile meat enzymolysis liquid are as follows: crocodile meat is cleaned, It drains, sterilize, crushing and be placed in sterile reaction kettle, the pure water of 2 times of crocodile meat weight is added into reaction kettle, albumen is added Enzyme and lipase, the dosage of enzyme are the 5% of total weight, are heated up to 65 DEG C, and be stirred continuously, and form crocodile meat enzymatic hydrolysis after digesting 5h Liquid.

It is further: in the step S2, to prepare the detailed process of crocodile bone meal are as follows: take fresh alligator bone, fleshing is gone forward side by side Row is preliminary broken, is put into saucepan the 5min that precooks, cleans up；Alligator bone after precooking is put into pressure cooker at 110 DEG C Boiling 2-3h, removal upper layer grease simultaneously drain, after alligator bone is put into baking oven, 2-3h is dried at 100-120 DEG C, after drying Alligator bone is taken out and carries out coarse crushing using pulverizer；Broken skeletal grain is put into micronizer after coarse crushing and carries out Ultramicro-powder It is broken, it sieves with 100 mesh sieve, obtains ultra micro crocodile bone meal, the repetition not being sieved continues to be sieved after crushing.

It is further: in the step S3, to prepare the detailed process of green tea powder are as follows: to take green tea to be placed under sunlight and carry out Spread, spread with a thickness of 2-4cm, time for spreading is 6-16h, and during which every 2-3h is stirred once；Tealeaves after sunning is put Enter in green-keeping machine and finish, control fixing temperature is 70-80 DEG C during water-removing, and the time of water-removing is 10min；After finishing Tealeaves be placed in rolling machine, then made in rolling machine using vacuum generator in negative pressure state, to tea under condition of negative pressure Leaf is rubbed, until so that the percentage of damage of tealeaves is reached 55-65%；Tealeaves after rubbing is placed in freeze drying box, -30 DEG C 3-5min is freezed under freezing environment, 3-5min is freezed under -40 DEG C of freezing environments, and microwave is carried out to freeze drying box after freezing Heat drying, dry temperature are 105-115 DEG C；Tealeaves after drying is placed in micronizer and is pulverized to obtain the final product Green tea powder.

It is further: in the step S3, to prepare the detailed process of modified Gorgon fruit are as follows: weigh appropriate Gorgon fruit be placed in it is sterile anti- It answers in pot, the purified water of 1.5 times of Gorgon fruit weight is then added, adds appropriate cellulase and pectase, reaction kettle is carried out Heating, temperature control are 46-50 DEG C, and the time of enzymatic hydrolysis is 1h；Gorgon fruit after enzymolysis processing is taken out from reaction pot and is placed in newly Reaction pot in, then be added Gorgon fruit total weight 120-160% Yoghourt, the citric acid of 4-8%, the malic acid of 3-5%, 4- 5% tomatin stirs and evenly mixs the microwave treatment for being placed on and carrying out specific frequency in micro-wave oven；By microblogging treated Gorgon fruit from It takes out in micro-wave oven, is dried with being placed in baking oven for pure water elution up to modified Gorgon fruit.

It is further: in the step S5, to prepare the detailed process of bunge auriculate root extract are as follows: select it is high-quality it is complete, without mould The radix cynanchi bungei root block of change, cleaning are diced after drying, and the radix cynanchi bungei root block after dicing is put into Titian impurity elimination, time in grilled machine For 5min, pulverization process is carried out afterwards, radix cynanchi bungei root block fine powder is made, then in the ratio of 1:8, be added into radix cynanchi bungei root tuber fine powder The ethyl alcohol that concentration is 78%, heats and carries out refluxing extraction 2 times, after extracting 1h, filtering, then filtrate is rotated to steaming at 58 DEG C Hair removes solvent, obtains bunge auriculate root extract.

Further: the revolving speed of Ultracentrifuge is 10000-12000rpm in the step S9, ultracentrifugation Time is 6-8min.

Further: in the step S10, all devices, pipeline, charging basket and whole outlet progress high temperature are killed before bottling Bacterium carries out filling, tamponade etc. with aseptic water washing bottle wine after bottling department sterilization processing, bottle placer stores vinasse with 100 DEG C of hot water It sterilizes half an hour, storage wine tube head is cleaned with 70% alcohol and sterilized, bottling department air ultraviolet sterilization；Pasteurizing temperature is 100 DEG C, time 10min.

Compared with prior art, the beneficial effects of the present invention are:

Alligator bone is made ultra micro bone meal, crocodile meat is made to crocodile meat enzymolysis liquid by the present invention, by crocodile bone meal and crocodile Meat enzymolysis liquid, which is put into white wine, to be impregnated, and crocodile bone and flesh health liquor is made, the nutritional ingredient of crocodile bone and flesh is made to have obtained filling sharp extraction And utilization, the bioactivity of the bioactive substance in animal tissue is maintained, lipoprotein, a variety of amino acid rich in animal, Vitamin etc., nutritive value is very high.

Green tea powder, Gorgon fruit and radix cynanchi bungei is added in the present invention during the preparation process, and tea polyphenols are most important activity in tealeaves One of ingredient, the bioactivity of tea polyphenols include anti-oxidant, anticancer, radiation protection, anti-mutation, the protection of cardiovascular disease, reduction Blood plasma cholesterol level, weight-reducing, increase bone density etc., the present invention is in the preparation process of green tea powder, and can adequately protect tea The activity of polyphenol, and the stability of chemical substance in tealeaves can be kept to the maximum extent；Gorgon fruit has supplementing the kidney to control the nocturnal, tonifying spleen stops Rush down, eliminating dampness and arresting leucorrhea the effect of, Gorgon fruit is impregnated by modified, and drug effect is easier to be absorbed by the body；Radix cynanchi bungei has nourishing Strong, blood-nourishing enriches blood, crow palpus dark hair, restrains the effect of vital essence, myogenic sore, relax bowel and defecation, joined hoary head in the present invention The extract of black root block is impregnated with white wine, and the pharmacological property of radix cynanchi bungei is easier to be absorbed by white wine.

The Chinese medicines such as jujube, lycium ruthenicum, American Ginseng are also added into the present invention, not only entrance is fine and smooth, and without Chinese medicine bitter taste, It is in good taste, it takes for a long time, there is the guarantor of tonifying kidney and strengthening yang, life essence-filling, marrow-benefitting, nourishing liver and kidney, blood-supplementing blood-nourishing, nourishing the blood and yin, blood-nourishing brain tonic Strong effect suitable for tired spirit, deficiency of vital energy and acking in strength, loss of appetite, insomnia forgetfulness, has a dizzy spell, has a pain in the back, fatigue and weakness etc. Disease.

Preparation method of the invention, process is simple, reduces the loss of material nutrient component, and improve raw material utilizes effect Rate is appropriate for producing in batches, be worthy to be popularized.

Specific embodiment

In order to deepen the understanding of the present invention, the present invention will be described in further detail with reference to the examples below, implements below Example for explaining only the invention, is not intended to limit the scope of the present invention..

Embodiment 1

A kind of crocodile bone and flesh health liquor, including following component by weight: 120 parts of crocodile meat enzymolysis liquid, alligator bone 150 parts of powder, 50 parts of jujube, 40 parts of lycium ruthenicum, 20 parts of green tea powder, modified 15 parts of Gorgon fruit, 10 parts of raspberry, 8 parts of Morinda officinalis, toast are sweet Careless 8 parts, 7 parts of American Ginseng, 6 parts of dark plum, 5 parts of Radix Astragali, 3 parts of bunge auriculate root extract, 4500 parts of white wine.

S1, preparation crocodile meat enzymolysis liquid: crocodile meat is cleaned, is drained, is sterilized, crushes and is placed on sterile reaction kettle It is interior, the pure water of 2 times of crocodile meat weight is added into reaction kettle, protease and lipase is added, the dosage of enzyme is total weight 5%, 65 DEG C are heated up to, and be stirred continuously, form crocodile meat enzymolysis liquid after digesting 5h.

S2, preparation crocodile bone meal: taking fresh alligator bone, and fleshing simultaneously be tentatively crushed, and is put into saucepan the 5min that precooks, clearly Wash clean；Alligator bone after precooking is put into pressure cooker the boiling 2h at 110 DEG C, removal upper layer grease simultaneously drains, after by crocodile Fish-bone is put into baking oven, and 2h is dried at 100 DEG C, is taken out alligator bone after drying and is carried out coarse crushing using pulverizer；Coarse crushing After broken skeletal grain be put into micronizer pulverize, sieve with 100 mesh sieve, obtain ultra micro crocodile bone meal, be not sieved Repetition crush after continue to be sieved.

S3, preparation green tea powder: taking green tea to be placed under sunlight and spread, spread with a thickness of 2cm, time for spreading For 6h, during which every 2h is stirred once；Tealeaves after sunning is put into green-keeping machine and is finished, control water-removing temperature during water-removing Degree is 70 DEG C, and the time of water-removing is 10min；Tealeaves after water-removing is placed in rolling machine, then makes to rub using vacuum generator It is in negative pressure state in twister, tealeaves is rubbed under condition of negative pressure, until so that the percentage of damage of tealeaves is reached 55%；It will rub Tealeaves after twirl is placed in freeze drying box, and 3min is freezed under -30 DEG C of freezing environments, and 3min is freezed under -40 DEG C of freezing environments, cold Micro-wave drying is carried out to freeze drying box after jelly, dry temperature is 105 DEG C；Tealeaves after drying is placed in ultra micro It carries out pulverizing up to green tea powder in pulverizer.

S4, it prepares modified Gorgon fruit: weighing appropriate Gorgon fruit and be placed in sterile reaction pot, 1.5 times of Gorgon fruit weight are then added Purified water adds appropriate cellulase and pectase, heats to reaction kettle, and temperature control is 46 DEG C, the time of enzymatic hydrolysis For 1h；Gorgon fruit after enzymolysis processing is placed in new reaction pot from taking-up in reaction pot, Gorgon fruit total weight 120% is then added Yoghourt, 4% citric acid, 3% malic acid, 4% tomatin, stir and evenly mix to be placed in micro-wave oven and carry out specific frequency Microwave treatment；By microblogging, treated that Gorgon fruit takes out from micro-wave oven, is dried with being placed in baking oven for pure water elution Up to modified Gorgon fruit.

S5, it preparing bunge auriculate root extract: selecting high-quality complete, the radix cynanchi bungei root block without mildew, cleaning is diced after drying, Radix cynanchi bungei root block after dicing is put into Titian impurity elimination in grilled machine, and time 5min carries out pulverization process afterwards, and radix cynanchi bungei is made Root block fine powder, then in the ratio of 1:8, the ethyl alcohol that concentration is 78% is added into radix cynanchi bungei root tuber fine powder, heats and flows back It extracts 2 times, after extracting 1h, filtering, then rotary evaporation, removing solvent obtain bunge auriculate root extract at 58 DEG C by filtrate.

S6, other pretreatments of raw material: jujube, lycium ruthenicum, raspberry, Morinda officinalis, radix glycyrrhizae preparata, the West are taken according to parts by weight Ginseng, dark plum, Radix Astragali are put into baking oven after cleaning, 30min are dried at 120 DEG C, is put into ball mill after drying and carries out ball milling, Mixture powder is obtained, mixture powder is stirred uniformly, mixed powder is obtained.

S7, an immersion treatment: taking white wine, crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder by weight, by white wine, Crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder are placed in wine jar, sealed soak 30d, and soaking temperature is 20 DEG C, obtain product A.

S8, secondary immersion treatment: taking modified Gorgon fruit, bunge auriculate root extract by weight, is added in product A, is added simultaneously The mixed powder that step S6 is obtained continues to impregnate 45d, and soaking temperature is 18 DEG C, obtains product B.

S9, ultracentrifugation processing: product B is placed in Ultracentrifuge, ultracentrifugation is carried out, takes supernatant i.e. Semi-finished product health liquor is obtained, wherein the revolving speed of Ultracentrifuge is 10000rpm, and the time of ultracentrifugation is 6min.

S10, filling process: filling, all devices, pipe before bottling are carried out to semi-finished product health liquor using aseptic filling technology Line, charging basket and all outlet carry out high temperature sterilization, with aseptic water washing bottle wine, carry out filling, tamponade after bottling department sterilization processing It is store the hot-water sterilization function half an hour that vinasse uses 100 DEG C Deng, bottle placer, the 70% alcohol scouring sterilization of storage wine tube head, bottling department air Use ultraviolet sterilization；Pasteurize is carried out to bottle after filling, obtains crocodile bone and flesh health liquor, pasteurizing temperature is 100 DEG C, when Between be 10min.

Embodiment 2

A kind of crocodile bone and flesh health liquor, including following component by weight: 135 parts of crocodile meat enzymolysis liquid, alligator bone 165 parts of powder, 60 parts of jujube, 50 parts of lycium ruthenicum, 25 parts of green tea powder, modified 20 parts of Gorgon fruit, 12 parts of raspberry, 10 parts of Morinda officinalis, toast 10 parts of Radix Glycyrrhizae, 10 parts of American Ginseng, 8 parts of dark plum, 6 parts of Radix Astragali, 4 parts of bunge auriculate root extract, 4750 parts of white wine；

S2, preparation crocodile bone meal: taking fresh alligator bone, and fleshing simultaneously be tentatively crushed, and is put into saucepan the 5min that precooks, clearly Wash clean；Alligator bone after precooking is put into pressure cooker the boiling 2.5h at 110 DEG C, removal upper layer grease simultaneously drains, after will Alligator bone is put into baking oven, and 2.5h is dried at 110 DEG C, is taken out alligator bone after drying and is carried out coarse crushing using pulverizer；Slightly Broken skeletal grain is put into micronizer after crushing to pulverize, is sieved with 100 mesh sieve, ultra micro crocodile bone meal is obtained, does not have The repetition of sieving continues to be sieved after crushing.

S3, preparation green tea powder: taking green tea to be placed under sunlight and spread, spread with a thickness of 3m, time for spreading For 10h, during which every 2.5h is stirred once；Tealeaves after sunning is put into green-keeping machine and is finished, control is killed during water-removing Green temperature is 75 DEG C, and the time of water-removing is 10min；Tealeaves after water-removing is placed in rolling machine, vacuum generator is then utilized Make to rub tealeaves under condition of negative pressure, until making the percentage of damage of tealeaves reach 60% in rolling machine in negative pressure state； Tealeaves after rubbing is placed in freeze drying box, 4min is freezed under -30 DEG C of freezing environments, is freezed under -40 DEG C of freezing environments 4min, carries out micro-wave drying to freeze drying box after freezing, and dry temperature is 110 DEG C；By the tealeaves after drying It is placed in micronizer and pulverize up to green tea powder.

S4, it prepares modified Gorgon fruit: weighing appropriate Gorgon fruit and be placed in sterile reaction pot, 1.5 times of Gorgon fruit weight are then added Purified water adds appropriate cellulase and pectase, heats to reaction kettle, and temperature control is 48 DEG C, the time of enzymatic hydrolysis For 1h；Gorgon fruit after enzymolysis processing is placed in new reaction pot from taking-up in reaction pot, Gorgon fruit total weight 140% is then added Yoghourt, 6% citric acid, 4% malic acid, 4.5% tomatin, stir and evenly mix to be placed in micro-wave oven and carry out specific frequency The microwave treatment of rate；By microblogging, treated that Gorgon fruit takes out from micro-wave oven, is done with being placed in baking oven for pure water elution Dry to obtain the final product modified Gorgon fruit.

S7, an immersion treatment: taking white wine, crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder by weight, by white wine, Crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder are placed in wine jar, sealed soak 30d, and soaking temperature is 22 DEG C, obtain product A.

S8, secondary immersion treatment: taking modified Gorgon fruit, bunge auriculate root extract by weight, is added in product A, is added simultaneously The mixed powder that step S6 is obtained continues to impregnate 45d, and soaking temperature is 20 DEG C, obtains product B.

S9, ultracentrifugation processing: product B is placed in Ultracentrifuge, ultracentrifugation is carried out, takes supernatant i.e. Semi-finished product health liquor is obtained, wherein the revolving speed of Ultracentrifuge is 11000rpm, and the time of ultracentrifugation is 7min.

Embodiment 3

A kind of crocodile bone and flesh health liquor, including following component by weight: 150 parts of crocodile meat enzymolysis liquid, alligator bone 180 parts of powder, 70 parts of jujube, 60 parts of lycium ruthenicum, 30 parts of green tea powder, modified 25 parts of Gorgon fruit, 15 parts of raspberry, 13 parts of Morinda officinalis, toast 12 parts of Radix Glycyrrhizae, 12 parts of American Ginseng, 10 parts of dark plum, 8 parts of Radix Astragali, 5 parts of bunge auriculate root extract, 5000 parts of white wine.

S2, preparation crocodile bone meal: taking fresh alligator bone, and fleshing simultaneously be tentatively crushed, and is put into saucepan the 5min that precooks, clearly Wash clean；Alligator bone after precooking is put into pressure cooker the boiling 3h at 110 DEG C, removal upper layer grease simultaneously drains, after by crocodile Fish-bone is put into baking oven, and 3h is dried at 120 DEG C, is taken out alligator bone after drying and is carried out coarse crushing using pulverizer；Coarse crushing After broken skeletal grain be put into micronizer pulverize, sieve with 100 mesh sieve, obtain ultra micro crocodile bone meal, be not sieved Repetition crush after continue to be sieved.

S3, preparation green tea powder: taking green tea to be placed under sunlight and spread, spread with a thickness of 4cm, time for spreading For 16h, during which every 3h is stirred once；Tealeaves after sunning is put into green-keeping machine and is finished, control water-removing during water-removing Temperature is 80 DEG C, and the time of water-removing is 10min；Tealeaves after water-removing is placed in rolling machine, is then made using vacuum generator It is in negative pressure state in rolling machine, tealeaves is rubbed under condition of negative pressure, until so that the percentage of damage of tealeaves is reached 65%；It will Tealeaves after rubbing is placed in freeze drying box, and 5min is freezed under -30 DEG C of freezing environments, freezes 5min under -40 DEG C of freezing environments, Micro-wave drying is carried out to freeze drying box after freezing, dry temperature is 115 DEG C；Tealeaves after drying is placed in super It carries out pulverizing up to green tea powder in atomizer.

S4, it prepares modified Gorgon fruit: weighing appropriate Gorgon fruit and be placed in sterile reaction pot, 1.5 times of Gorgon fruit weight are then added Purified water adds appropriate cellulase and pectase, heats to reaction kettle, and temperature control is 50 DEG C, the time of enzymatic hydrolysis For 1h；Gorgon fruit after enzymolysis processing is placed in new reaction pot from taking-up in reaction pot, Gorgon fruit total weight 160% is then added Yoghourt, 8% citric acid, 5% malic acid, 5% tomatin, stir and evenly mix to be placed in micro-wave oven and carry out specific frequency Microwave treatment；By microblogging, treated that Gorgon fruit takes out from micro-wave oven, is dried with being placed in baking oven for pure water elution Up to modified Gorgon fruit.

S7, an immersion treatment: taking white wine, crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder by weight, by white wine, Crocodile meat enzymolysis liquid, crocodile bone meal and green tea powder are placed in wine jar, sealed soak 30d, and soaking temperature is 24 DEG C, obtain product A.

S8, secondary immersion treatment: taking modified Gorgon fruit, bunge auriculate root extract by weight, is added in product A, is added simultaneously The mixed powder that step S6 is obtained continues to impregnate 45d, and soaking temperature is 22 DEG C, obtains product B.

S9, ultracentrifugation processing: product B is placed in Ultracentrifuge, ultracentrifugation is carried out, takes supernatant i.e. Semi-finished product health liquor is obtained, wherein the revolving speed of Ultracentrifuge is 12000rpm, and the time of ultracentrifugation is 8min.

Comparative example

Common commercially available health liquor.

Experimental example

In order to compare effect of the present invention, the present invention carries out stomach-filling experiment to SPF grades of mouse, specifically:

1, experimental system is established: buying 50 from Wuhan University's animal experimental center, constitution size is almost completely the same They are randomly divided into 5 groups, every group after SPF grades of animal houses adapt to feed 7 days by the SPF grade mouse at 50 2 healthy monthly ages 10 mouse, then respectively with the health care of embodiment 1, embodiment 2, the health liquor of the method for embodiment 3 preparation and comparative example Wine distinguishes stomach-filling to every group of mouse after diluting 100 times respectively, and the stomach-filling time is every morning 9:00-9:30, continues stomach-filling 6 weeks, All free diet drinking-water of mouse during experiment.

2, the processing of serum: fasting 12h after mouse last time stomach-filling, free water, eyeball take blood, are placed in containing 1% In the centrifuge tube of heparin sodium, 40C, 5000rpm are centrifuged 15min, collect blood plasma.

3, SOD viability examination: according to SOD kit, the blood plasma prepared with step 2, the SOD for measuring every group of experiment mice lives Power, and statistical experiment result.

Specific experiment correlation data is as shown in table 1 below:

Table 1

	SOD in serum content (U/mL)
		Embodiment 1	151±5.12
Embodiment 2	149±5.23
		Embodiment 3	153±4.68
Comparative example	111±4.85

As seen from the above table, the present invention obtained by health liquor have anti-oxidant, delaying senility, nourishing liver and kidney, blood-supplementing blood-nourishing, Equal health-care efficacies, have promotional value.

The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and what is described in the above embodiment and the description is only the present invention Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these variation and Improvement is both fallen in the range of claimed invention.The present invention claims protection scope by appended claims and its Equivalent defines.

Claims

1. a crocodile bone meat health-care wine, is characterized in that, comprises the following components by weight: 120-150 parts of crocodile meat enzymolysis solution, 150-180 parts of crocodile bone meal, 50-70 parts of red dates, 40-150 parts of black wolfberry 60 servings, green tea powder 20-30 servings, modified Gorgon 15-25 servings, raspberry 10-15 servings, Morinda officinalis 8-13 servings, licorice root 8-12 servings, American ginseng 7-12 servings, ebony 6-10 servings parts, 5-8 parts of Astragalus, 3-5 parts of Baishouwu extract, 4500-5000 parts of liquor.

2. a preparation method of crocodile bone meat health wine, is characterized in that, specifically comprises the following steps:

S1, prepare crocodile meat enzymolysis solution: take fresh crocodile meat, and prepare crocodile meat enzymolysis solution;

S2, prepare crocodile bone meal: take fresh crocodile bone to prepare crocodile bone meal;

S3, prepare green tea powder: take fresh, high-quality green tea leaves without rotten leaves, and prepare green tea powder;

S4, preparation of modified Gorgon: take an appropriate amount of Gorgon, and prepare the modified Gorgon;

S5, preparation of Baishouwu extract: select high-quality, intact, mildew-free tuberous roots of Baishouwu, and prepare Baishouwu extract;

S6. Pretreatment of other raw materials: pretreatment of raw materials of other components to obtain mixed powder;

S7. One-time soaking treatment: take liquor, crocodile meat enzymolysis solution, crocodile bone meal and green tea powder by weight, put the liquor, crocodile meat enzymolysis solution, crocodile bone meal and green tea powder in a wine jar, seal and soak for 30 days, soaking temperature is 20-24 ° C to obtain product A;

S8, secondary soaking treatment: take the modified Gorgon fruit and Baishouwu extracts by weight, add them to the product A, add the mixed powder obtained in step S6 at the same time, continue soaking for 45d, and the soaking temperature is 18-22 ° C to obtain the product B;

S9, ultra-high-speed centrifugation: product B is placed in the ultra-high-speed centrifuge, carries out ultra-high-speed centrifugation, gets supernatant and promptly obtains semi-finished health care wine;

S10. Filling treatment: The semi-finished health wine is filled with aseptic filling technology, and the wine bottle is pasteurized after filling to obtain crocodile bone meat health wine.

3. the preparation method of a kind of crocodile meat health wine according to claim 2, is characterized in that, in described step S1, the concrete process of preparing crocodile meat enzymolysis solution is: crocodile meat is cleaned, drained, sterilized 2. After crushing, place it in a sterile reactor, add 2 times the weight of crocodile meat in pure water, add protease and lipase, the amount of enzyme is 5% of the total weight, heat to 65 ° C, and keep stirring , the crocodile meat enzymolysis solution was formed after enzymolysis for 5h.

4. the preparation method of a kind of crocodile bone and meat health wine according to claim 2, is characterized in that, in described step S2, the concrete process of preparing crocodile bone meal is: get fresh crocodile bone, remove meat and carry out preliminary crushing, put Put the pre-cooked crocodile bones in a pressure cooker for 5 minutes and cook them for 2-3 hours at 110°C, remove the upper layer of grease and drain, and then put the crocodile bones in the oven at 100-120 Dry at ℃ for 2-3 hours. After drying, take out the crocodile bone and use a pulverizer for coarse pulverization; after the coarse pulverization, put the broken bone particles into an ultrafine pulverizer for ultrafine pulverization, and pass through a 100-mesh sieve to obtain an ultrafine crocodile. Bone meal, repeat crushing without sieving and continue sieving.

5. the preparation method of a kind of crocodile bone and meat health wine according to claim 2, is characterized in that, in described step S3, the concrete process of preparing green tea powder is: get green tea leaves and place under sunlight and spread, spread The thickness of the laying is 2-4cm, the laying time is 6-16h, and the tea leaves are turned every 2-3h during the period; the dried tea leaves are put into the fixing machine for fixing, and the fixing temperature is controlled at 70-80 ℃ during the fixing process. The greening time is 10 minutes; the green tea leaves are placed in the rolling machine, and then the vacuum generator is used to make the rolling machine in a negative pressure state, and the tea leaves are rolled under the negative pressure condition, so that the breaking rate of the tea leaves reaches 55-65 %; put the rolled tea leaves in a freeze-drying box, freeze for 3-5 minutes in a freezing environment of -30 °C, freeze for 3-5 minutes in a freezing environment of -40 °C, and dry the freeze-drying box by microwave heating after freezing. The temperature is 105-115°C; the dried tea leaves are placed in an ultrafine pulverizer for ultrafine pulverization to obtain green tea powder.

6. the preparation method of a kind of crocodile bone meat health wine according to claim 2, is characterized in that, in described step S3, the concrete process of preparing modified Gorgon is: take by weighing an appropriate amount of Gorgon and place in aseptic reaction pot, Then add 1.5 times the weight of gorgonians in purified water, then add an appropriate amount of cellulase and pectinase, heat the reaction kettle, control the temperature to 46-50℃, and the enzymatic hydrolysis time is 1h; Take out the reaction pot and place it in a new reaction pot, then add 120-160% yogurt, 4-8% citric acid, 3-5% malic acid, 4-5% tomato in the total weight of Gorgon, stir and mix. After being uniformly placed in a microwave oven, microwave treatment at a specific frequency is carried out; the micro-blog treated gorgon is taken out from the microwave oven, rinsed with pure water, and then placed in an oven for drying to obtain modified gorgon.

7. the preparation method of a kind of crocodile bone and meat health wine according to claim 2, is characterized in that, in described step S5, the concrete process of preparing Baishouwu extract is: select high-quality intact, mildew-free Baishouwu Root block, washed and sun-dried, cut into cubes, put the diced Baishouwu root block into a charcoal roasting machine to remove impurities, the time is 5min, and then pulverized to obtain a finely divided Baishouwu root block. Then add 78% ethanol to the fine powder of Baishouwu root tuber at a ratio of 1:8, heat and perform reflux extraction for 2 times, after extraction for 1 hour, filter, and then rotate the filtrate at 58 °C to evaporate, The solvent was removed to obtain Baishouwu extract.

8. the preparation method of a kind of crocodile bone meat health wine according to claim 2, is characterized in that, in described step S9, the rotating speed of ultracentrifuge is 10000-12000rpm, and the time of ultracentrifugation is 6-8min.

9. the preparation method of a kind of crocodile bone meat health wine according to claim 2, is characterized in that, in described step S10, before bottling, all equipment, pipelines, buckets and all outlets carry out high temperature sterilization, use sterile water Rinse the bottle of wine, and carry out filling and corking after sterilization in the filling room. The wine storage tank of the filling machine is sterilized with 100 ℃ hot water for half an hour. The head of the wine storage pipe is scrubbed and sterilized with 70% alcohol. Ultraviolet sterilization; pasteurization temperature is 100 ℃, time is 10min.