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CN109601058B - Method for cultivating strong seedlings of delayed-cultivation watermelons in autumn - Google Patents

Method for cultivating strong seedlings of delayed-cultivation watermelons in autumn Download PDF

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CN109601058B
CN109601058B CN201910051854.5A CN201910051854A CN109601058B CN 109601058 B CN109601058 B CN 109601058B CN 201910051854 A CN201910051854 A CN 201910051854A CN 109601058 B CN109601058 B CN 109601058B
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watermelon
autumn
seedlings
methyl jasmonate
seeds
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CN109601058A (en
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黄雯
魏猷刚
甘小虎
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NANJING INSTITUTE OF VEGETABLE SCIENCE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps: soaking watermelon seeds in a methyl jasmonate aqueous solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the watermelon seeds germinate; the germinated watermelon seeds are soaked in methyl jasmonate aqueous solution for 1-3 hours and then used for plug seeding, and the watermelon seedlings can be transplanted after growing into 3 leaves and 1 heart, and are used for cultivation production of delayed cultivation watermelons in autumn. The method for cultivating strong watermelon seedlings in late autumn can effectively inhibit the spindly growth of the watermelon seedlings in late autumn, enhance the heat resistance and disease resistance of the watermelon seedlings, improve the strong seedling rate, and is simple to operate, time-saving, labor-saving and low in cost.

Description

Method for cultivating strong seedlings of delayed-cultivation watermelons in autumn
Technical Field
The invention belongs to the field of plant cultivation, and particularly relates to a method for cultivating strong seedlings of delayed-cultivation watermelons in autumn.
Background
9-11 months per year is the traditional low season of watermelon production, however, in the mid-autumn and national holidays, and at this time, partial residents in the southeast coastal areas have the habit of biting autumn watermelon, the consumption demand of the watermelon is still large, and therefore, in order to meet the market demand of the watermelon, the area of the watermelon cultivated in autumn in a delayed manner is gradually increased. The seedlings of the delayed watermelon cultivation in autumn are generally cultivated in summer (summer seedling cultivation), the problems of excessive growth of seedlings, heat stress injury and diseases, extremely low strong seedling rate and the like are most easily caused in the high-temperature and high-humidity environment, and the development of the delayed watermelon cultivation in autumn is greatly limited.
The excessive growth of watermelon seedlings is mainly characterized by slender watermelon stems, poor root system development, poor disease resistance and resistance, low survival rate after planting, long seedling revival time, easy formation of weak seedlings and serious influence on the quality of seedlings. Because the morphogenesis of watermelon seedlings is an irreversible process, the robustness of the cultured seedlings directly influences the growth and development of plants and is closely related to the yield and quality of crops. The traditional technology for controlling the excessive growth of watermelon seedlings is to spray paclobutrazol in the seedling stage, and in the actual production, because of variable environmental factors and difference among cultivated varieties, the effect is not good or the later-stage growth of watermelon seedlings is affected because of improper selection of the paclobutrazol. The dosage of the pesticide for spraying in the seedling stage is very large, the pesticide is difficult to absorb by plants, soil residue is easy to cause, the growth of the next-stubble crops can be influenced, and the pesticide is not suitable for long-term continuous use. Therefore, the invention provides a safe and effective method for inhibiting excessive growth of watermelon seedlings in autumn, and has important significance for cultivating strong seedlings.
Heat stress is a common environmental stress, and is most likely to occur in the late autumn watermelon seedling culture in summer high-temperature weather. The watermelon seedlings have weak resistance, if water is not supplied timely in high-temperature weather, the watermelon seedlings easily cause certain damage to seedling roots and leaves, the water and nutrition are not completely absorbed, the leaves are blocked from growing, the seedlings grow slowly, and strong seedlings are difficult to form. Therefore, the resistance of the watermelon seedlings to heat stress is improved, and the method has important significance for cultivating strong seedlings.
The main diseases of watermelon in seedling stage are damping-off and damping-off of watermelon, and the watermelon is damaged and suddenly falls down to die or stands up to die, so that huge seedling loss is caused. The method for preventing and treating damping-off and damping-off of the watermelons comprises the steps of treating seeds with a bactericide or spraying the bactericide after emergence of the seedlings, but the treatment of the seeds with the bactericide easily affects the germination of the seeds and the growth vigor of the seedlings at the later stage, the spraying of the bactericide after emergence of the seedlings can increase the labor cost and the economic cost, and the prevention and treatment effect is poor.
Methyl jasmonate is widely present in plants, and researches show that methyl jasmonate has the functions of inhibiting seed germination, inhibiting growth, inducing expression of defense genes, enhancing stress resistance and disease resistance of plants and the like. Although some reports related to exogenous application of methyl jasmonate for inhibiting plant growth or enhancing plant resistance exist, the application mode mostly adopts a liquid spraying mode, the method is difficult for plants to absorb medicaments, the spraying mode is mostly repeated, the waste is large, the medicament demand is very large, and the methyl jasmonate cannot be applied in the actual production due to the high price of the methyl jasmonate. Methyl jasmonate has the function of inhibiting seed germination, is easy to cause the problems of low seed germination rate or poor effect of inhibiting plant growth and the like due to improper use concentration during seed soaking, and has not been reported in the cultivation of autumn strong watermelon seedlings. Therefore, a reasonable use method of methyl jasmonate is developed and used for cultivating strong autumn watermelon seedlings, the functions of inhibiting growth and enhancing resistance of the strong autumn watermelon seedlings are fully utilized, and the method is significant in the watermelon seedling cultivation industry.
Therefore, on the premise of not changing the existing seedling culture process, a time-saving, labor-saving and low-cost method for culturing strong autumn watermelon seedlings is developed, the problems of excessive growth, poor heat resistance, serious diseases and the like in the existing seedling culture are solved, and the method has important significance for the development of the autumn delayed watermelon cultivation industry.
Disclosure of Invention
The technical problem is as follows: in order to solve the defects of the prior art, the invention provides a method for cultivating strong seedlings of delayed watermelon in autumn.
The technical scheme is as follows: a method for cultivating strong seedlings of delayed-cultivated watermelons in autumn comprises the following steps:
step 1, soaking watermelon seeds in a low-concentration methyl jasmonate solution for 5-7 hours, wrapping the watermelon seeds with a wrapper with good air permeability and moisture retention, and putting the wrapped watermelon seeds into a germination accelerating box until the watermelon seeds germinate;
and 2, placing the germinated watermelon seeds in a low-concentration methyl jasmonate solution, continuously soaking for 1-3 hours, sowing, transplanting after the sowed watermelon seedlings grow into strong seedlings, and carrying out cultivation production of delayed watermelons in autumn.
As a preferable scheme: in the step 1, watermelon seeds are soaked in a low-concentration methyl jasmonate solution for 6 hours, wrapped by a wrapper with good air permeability and moisture retention, and placed in a germination accelerating box until the watermelon seeds germinate.
As a further preferable scheme: the methyl jasmonate solution in the step 1 and the step 2 has the same concentration and comprises the following components in parts by volume: 0.01-0.03 part of methyl jasmonate, 0.1 part of ethanol and 99.87-99.89 parts of water.
As a further preferable scheme: and 2, placing the germinated watermelon seeds in a low-concentration methyl jasmonate solution, continuously soaking for 2 hours, and then sowing.
As a further preferable scheme: the methyl jasmonate solution comprises the following components in parts by volume: 0.02 part of methyl jasmonate, 0.1 part of ethanol and 99.88 parts of water.
As a further preferable scheme: the preparation process of the methyl jasmonate solution is as follows: weighing a certain amount of methyl jasmonate, adding the methyl jasmonate into a certain amount of ethanol, fully and uniformly mixing the methyl jasmonate and the ethanol, transferring the mixture into a constant volume device, and continuously adding water into the constant volume device to a required volume to obtain a methyl jasmonate solution with a required concentration.
As a further preferable scheme: in the step 1, the wrapping material with good air permeability and moisture retention is gauze.
As a further preferable scheme: the set temperature of the germination accelerating box in the step 1 is 25 ℃.
As a further preferable scheme: and 2, the standard of strong seedling transplanting of the Chinese and western melon seedlings is that the Chinese and western melon seedlings grow to 3 leaves and 1 heart.
As a further preferable scheme: and in the step 2, the medium for sowing the watermelon seeds is a hole tray.
Has the advantages that: according to the method for cultivating strong watermelon seedlings in late autumn provided by the invention, in the conventional method for cultivating watermelon seedlings, excessive growth of watermelon seedlings can be inhibited and the heat resistance and disease resistance of the watermelon seedlings can be enhanced through two simple soaking treatments of methyl jasmonate, so that the aim of cultivating strong watermelon seedlings in autumn is fulfilled. The method for cultivating the strong watermelon seedlings in the delayed autumn with time, labor and low cost has important significance for the development of the industry for cultivating the watermelons in the delayed autumn.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to limit the scope of the invention, as various equivalent modifications of the invention will occur to those skilled in the art upon reading the present disclosure and fall within the scope of the appended claims.
The invention relates to a method for cultivating strong seedlings of delayed-cultivated watermelons in autumn, which comprises the following steps of:
step 1, soaking watermelon seeds in a low-concentration methyl jasmonate solution for 6 hours, wrapping the watermelon seeds with gauze, and putting the watermelon seeds into a germination accelerating box at 25 ℃ until the watermelon seeds germinate;
and 2, placing the germinated watermelon seeds in a low-concentration methyl jasmonate solution, continuously soaking for 2 hours, performing plug seeding, transplanting the watermelon seedlings after the sowed watermelon seedlings grow to 3 leaves and 1 heart, and performing cultivation production of delayed watermelons in autumn.
Preparing a methyl jasmonate solution: weighing 20 μ L of methyl jasmonate, adding into 100 μ L of ethanol, mixing the two solutions, transferring into a volumetric flask, adding 99.88ml of water until the total volume of the solution is 100ml, and mixing.
In the embodiment, the methyl jasmonate and the ethanol are both pure methyl jasmonate and pure ethanol which are purchased from laboratories, and the manufacturers and the types are as follows (the same below):
methyl jasmonate (Sigma-Aldrich) (CAS number 39924-52-2);
ethanol (national pharmaceutical group chemical Co., Ltd.) (CAS number: 64-17-5).
Example 1
The embodiment provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 5 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of methyl jasmonate solution for 1 hour and then used for plug seeding, and the watermelon seedlings can be transplanted after growing to 3 leaves and 1 heart, and can be used for cultivation production of autumn delayed cultivation watermelons.
The methyl jasmonate solution in the step 1 and the step 2 is the same water solution, and the preparation method is that 10 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Example 2
The embodiment provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of methyl jasmonate solution for 2 hours and then used for plug seeding, and the watermelon seedlings can be transplanted after growing to 3 leaves and 1 heart, and can be used for cultivation production of autumn delayed cultivation watermelons.
The methyl jasmonate solution in the step 1 and the step 2 is the same water solution, and the preparation method is that 20 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Example 3
The embodiment provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 7 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of methyl jasmonate solution for 3 hours and then used for plug seeding, and the watermelon seedlings can be transplanted after growing to 3 leaves and 1 heart, and can be used for cultivation production of autumn delayed watermelon.
The methyl jasmonate solution in the step 1 and the step 2 is the same water solution, and the preparation method is that 30 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Example 4
The embodiment provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of methyl jasmonate solution for 3 hours and then used for plug seeding, and the watermelon seedlings can be transplanted after growing to 3 leaves and 1 heart, and can be used for cultivation production of autumn delayed cultivation watermelons.
The methyl jasmonate solution in the step 1 and the step 2 is the same water solution, and the preparation method is that 10 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Example 5
The embodiment provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of methyl jasmonate solution for 1 hour and then used for plug seeding, and the watermelon seedlings can be transplanted after growing to 3 leaves and 1 heart, and can be used for cultivation production of autumn delayed cultivation watermelons.
The methyl jasmonate solution in the step 1 and the step 2 is the same water solution, and the preparation method is that 30 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Comparative example 1
The comparative example provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of water for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are used for plug seeding, and the watermelon seedlings can be transplanted after growing into 3 leaves and 1 heart, and can be used for cultivation production of delayed autumn watermelon cultivation.
Comparative example 2
The comparative example provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of water for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
(2) the germinated watermelon seeds are soaked in 100ml of water for 2 hours and then used for plug seeding, and the watermelon seedlings can be transplanted after growing into 3 leaves and 1 heart, and can be used for cultivation production of delayed autumn watermelon cultivation.
Comparative example 3
The comparative example provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
the preparation method of the methyl jasmonate solution in the step 1 is that 20 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
Comparative example 4
The comparative example provides a method for cultivating late-cultivated strong watermelon seedlings in autumn, which comprises the following steps:
(1) soaking 100 watermelon seeds in 100ml of methyl jasmonate solution for 6 hours, wrapping the seeds with gauze, and putting the wrapped seeds into a germination accelerating box at about 25 ℃ until the seeds germinate;
the preparation method of the methyl jasmonate aqueous solution in the step 1 is that 60 mu L of methyl jasmonate is added into 100 mu L of ethanol, fully and uniformly mixed, and then water is added to 100 ml.
And (4) counting results:
the germination rates of the seeds in the examples 1-5 and the comparative examples 1-4 are measured, and the height, the stem thickness, the strong seedling index and the disease incidence rate of the watermelon seedling are measured when the watermelon seedling has three leaves and one heart, wherein the strong seedling index calculation method comprises the following steps: (stem thickness/plant height + dry root weight/dry aerial part weight) × dry whole plant weight. The watermelon seedlings are placed in an artificial climate box for simulation high-temperature treatment when the watermelon seedlings have three leaves and one heart, the temperature is 35 ℃ day and night, the water conditions are kept consistent among the treatments, and sampling is carried out after high-temperature stress is carried out for 5 days to measure the conductivity for identifying the heat resistance.
Table 1: growth status, Heat resistance and disease resistance of the seedlings of the Chinese and Western melons in each example and comparative example
Figure BDA0001950994540000061
As can be seen from Table 1, the stem thickness and strong seedling indexes of the examples 1 to 5 are obviously higher than those of the comparative example 1 and the comparative example 2, and the conductivity and the disease incidence are obviously lower than those of the comparative example 1 and the comparative example 2, which shows that the method for cultivating late-cultivated watermelon strong seedlings in autumn of the invention is obviously superior to the existing seedling method, and has the effects of inhibiting excessive growth, cultivating strong seedlings and enhancing the heat resistance and disease resistance of seedlings.
The seedling growth index of example 2 is slightly higher than that of example 1, example 3, example 4 and example 5, which shows that the concentration of methyl jasmonate and the treatment time are optimal in example 2.
The seed germination rate of example 2 was the same as that of comparative example 2, indicating that the jasmonic acid solution seed soaking at low concentration did not affect the seed germination rate.
The stem thickness and the seedling strengthening index of example 2 are both higher than those of comparative example 3, which shows that the effect of two treatments with jasmonic acid solution under the same concentration is obviously better than that of a single treatment for soaking seeds.
Comparative example 4 the treated strong seedling index is obviously higher than comparative example 2 and degree ratio 3, but the seed germination rate is lower than comparative example 2 and comparative example 3, which shows that the seed soaking with high-concentration jasmonic acid solution can inhibit the excessive growth of seedlings, but can inhibit the seed germination and influence the germination rate.
The seed germination rate and the seedling strengthening index of example 2 are obviously superior to those of comparative example 4, which shows that 2 times of low-concentration methyl jasmonate treatment is superior to 1 time of high-concentration seed soaking treatment.
In summary, the following steps: aiming at the problems of excessive growth of seedlings, poor heat resistance and disease resistance, low seedling strengthening rate and the like in the conventional method for cultivating late cultivation watermelon seedlings in autumn (summer seedling cultivation), on the basis of the conventional seedling cultivation process, substances which are easy to cause damage such as paclobutrazol, bactericide and the like are not used, and the functions of inhibiting growth and enhancing resistance of methyl jasmonate are exerted only by 2 times of soaking treatment of methyl jasmonate with lower concentration, so that the aim of cultivating late cultivation watermelon strong seedlings in autumn is fulfilled. The same solution is used for soaking before and after germination respectively, so that the problem that the seed germination inhibition or growth inhibition effect is not obvious when methyl jasmonate is soaked once is solved, and the problems of difficult plant absorption, large waste, high cost and the like in the existing spraying and using process of methyl jasmonate are solved. The method can inhibit the excessive growth of the seedling stage of the delayed cultivation watermelon in autumn, simultaneously enhance the heat resistance and the disease resistance of the watermelon seedlings, achieve the aim of cultivating the strong seedlings of the delayed cultivation watermelon in autumn, and is a time-saving, labor-saving, low-cost and safe method for cultivating the strong seedlings of the delayed cultivation watermelon in autumn.

Claims (9)

1. A method for cultivating strong seedlings of delayed watermelon in autumn is characterized in that: the method comprises the following steps:
step 1, soaking watermelon seeds in a low-concentration methyl jasmonate solution for 5-7 hours, wrapping the watermelon seeds with a wrapper with good air permeability and moisture retention, and putting the wrapped watermelon seeds into a germination accelerating box until the watermelon seeds germinate;
step 2, placing the germinated watermelon seeds into a low-concentration methyl jasmonate solution, continuing to soak for 1-3 hours, then sowing, transplanting after the sowed watermelon seedlings grow strong, carrying out cultivation production of delayed watermelons in autumn,
the low-concentration methyl jasmonate solution in the step 1 and the step 2 comprises the following components in parts by volume: 0.01-0.03 part of methyl jasmonate, 0.1 part of ethanol and 99.87-99.89 parts of water.
2. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: in the step 1, the watermelon seeds are soaked in a low-concentration methyl jasmonate solution for 6 hours, wrapped by a wrapper with good air permeability and moisture retention, and placed in a germination accelerating box until the watermelon seeds germinate.
3. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: and 2, placing the germinated watermelon seeds in a low-concentration methyl jasmonate solution, continuously soaking for 2 hours, and then sowing.
4. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: the methyl jasmonate solution comprises the following components in parts by volume: 0.02 part of methyl jasmonate, 0.1 part of ethanol and 99.88 parts of water.
5. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 4, wherein the method comprises the following steps: the preparation process of the methyl jasmonate solution is as follows: weighing a certain amount of methyl jasmonate, adding the methyl jasmonate into a certain amount of ethanol, fully and uniformly mixing the methyl jasmonate and the ethanol, transferring the mixture into a constant volume device, continuously adding water into the constant volume device to a required volume, and uniformly mixing to obtain a methyl jasmonate solution with a required concentration.
6. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: and in the step 1, the wrapping with good air permeability and moisture retention is gauze.
7. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: the temperature set in the germination accelerating box in the step 1 is 25 ℃.
8. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: and in the step 2, the standard of strong seedling transplantation of the Chinese and western melon seedlings is that the Chinese and western melon seedlings grow to 3 leaves and 1 heart.
9. The method for cultivating the strong watermelon seedlings delayed for cultivation in autumn as claimed in claim 1, wherein the method comprises the following steps: and in the step 2, the medium for sowing the watermelon seeds is a hole tray.
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