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CN109566626A - A kind of bactericidal composition - Google Patents

A kind of bactericidal composition Download PDF

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Publication number
CN109566626A
CN109566626A CN201710909455.9A CN201710909455A CN109566626A CN 109566626 A CN109566626 A CN 109566626A CN 201710909455 A CN201710909455 A CN 201710909455A CN 109566626 A CN109566626 A CN 109566626A
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CN
China
Prior art keywords
plant
bactericidal composition
cyflufenamid
prevention
disease
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CN201710909455.9A
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Chinese (zh)
Inventor
罗昌炎
詹姆斯.T.布里斯托
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Jiangsu Rotam Chemical Co Ltd
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Jiangsu Rotam Chemical Co Ltd
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Priority to CN201710909455.9A priority Critical patent/CN109566626A/en
Publication of CN109566626A publication Critical patent/CN109566626A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/52—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing groups, e.g. carboxylic acid amidines

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of bactericidal composition, and the bactericidal composition contains active constituent fluorine azoles bacterium acyl azanol and cyflufenamid, and wherein the weight percent of fluorine azoles bacterium acyl azanol and cyflufenamid is 50:1-1:50.The invention further relates to the bactericidal composition be used to control Cereal, veterinary antibiotics, on ornamental plant and grapevine harmful fungoid and bacterium purposes.The purposes of pathogenic or saprophytic fungi and bacterium in the place prevention and treatment soil or cultivation medium prevented and treated needed for being applied to the invention further relates to the bactericidal composition.It is used to handle seed the invention further relates to the bactericidal composition to be protected from the purposes of the phytopathogen invasion of seed carrying.

Description

A kind of bactericidal composition
Technical field
The present invention relates to a kind of bactericidal compositions；The invention further relates to use the bactericidal composition agricultural or gardening Application in terms of field prevents and treats or prevention plant pathogenic fungi infects plant.
Background technique
About pesticide activity, especially to crop protection, the key problem for the research carried out in the technical field first is that Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.
Fluorine azoles bacterium acyl azanol (Pydiflumetofen), chemical name are 3- difluoromethyl -1- methyl-1 H- pyrazoles -4- Carboxylic acid methoxy-[1- methyl -2-(2,4,6- trichlorophenyl)-ethyl]-amide.Fluorine azoles bacterium acyl hydroxylamine compound is by Syngenta The formamide microbicide of company's exploitation.Fluorine azoles bacterium acyl azanol discloses in WO2010/063700.Fluorine azoles bacterium acyl azanol There is higher activity to wheat scab, powdery mildew of strawberry, leaf muld of tomato, sigatoka, banana freckle.Fluorine azoles bacterium Acyl azanol structural formula is as follows:
A kind of long-term reactive compound of single administration, which carrys out controlling disease, will lead to disease drug resistance generation, cause under compound preventive effect Drop, or even thoroughly lose preventive effect.In addition, there are also plant protection art, there are also to activity profile, toxicity, selectivity, rate of application, remnants Requirement in terms of object composition and advantageous preparation feasibility.Therefore, exploitation is killed better than the new of existing fungicide in some aspects Microbial inoculum is lasting task.
Summary of the invention
Object of the present invention is to provide a kind of bactericidal composition in view of the above shortcomings, the composition is by by fluorine azoles bacterium acyl Azanol and cyflufenamid are combined, so that obtained composition has gain effect in control efficiency.
It has now unexpectedly been found that simultaneously, i.e., jointly or separately applying fluorine azoles bacterium acyl azanol and cyflufenamid, or successively apply Fluorine azoles bacterium acyl azanol and cyflufenamid preferably to prevent and treat plant pathogenic fungi than each compound is administered alone.
The combination of fluorine azoles bacterium acyl azanol and cyflufenamid, not only further expansion is to it is generally desirable to by the plant prevented and treated The action spectrum of pathogen, and obtain synergistic effect.
The present invention provides a kind of bactericidal composition, the composition is by carrying out group for fluorine azoles bacterium acyl azanol and cyflufenamid It closes, so that obtained composition has gain effect in control efficiency, and has expanded fungicidal spectrum, effectively slowed down or avoid disease Bacterium develops drug resistance.
Astoundingly, the bactericidal activity of bactericidal composition of the invention is brighter than the active adduction of each reactive compound It is aobvious higher.In other words, there are unpredictable, necessary being synergistic effects, rather than just active supplement.
A kind of bactericidal composition of the present invention is to adopt the following technical solutions to achieve:
A kind of bactericidal composition, it is characterised in that: the bactericidal composition contains active constituent fluorine azoles bacterium acyl azanol and ring fluorine bacterium Amine, wherein the weight percent of fluorine azoles bacterium acyl azanol and cyflufenamid be 50:1-1:50, further preferably 40:1-1:40, then Preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 10:1-1:10, more preferable 5:1-1:5.
In the present invention, the weight percent of fluorine azoles bacterium acyl azanol and cyflufenamid can also be 50:1,49:1,48:1,47: 1,46:1,45:1,44:1,43:1,42:1,41:1,40:1,39:1,38:1,37:1,36:1,35:1,34:1,33:1,32:1, 31:1,30:1,29:1,28:1,27:1,26:1,25:1,24:1,23:1,22:1,21:1,20:1,19:1,18:1,17:1, 16:1,15:1,14:1,13:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1.5:1,1:1, 1:1.5,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1: 17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31,1: 32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1: 47,1:48,1:49,1:50。
Fluorine azoles bacterium acyl azanol is also possible to its tautomer；Or exist in the form of hydrate or its solvate.
A kind of bactericidal composition also includes filler and/or table containing active constituent fluorine azoles bacterium acyl azanol and cyflufenamid Face activating agent.
The bactericidal composition, dosage form be aerosol, capsule suspension, harl concentrating agents, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, instant solution, sprayable pulvis, emulsifiable concentrating agents, oil-in-water emulsion, water-in-oil emulsion, Bulky grain agent, fine granule, the dispersible powder of oil, the miscible flowable concentrating agents of oil, oily miscible liquids, foaming agent, paste, Suspension concentrating agents, solvable concentrating agents, suspending agent, seed coat agent, wettable powder, water dispersible granules, soluble powder, microcapsule suspension Agent, squeezes out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids, suspoemulsion, super-low capacity at coated granule Measure cold atomization preparation, super-low capacity calorimetric atomization preparation, double pack (twin pack), seed treatment dry powder doses, seed treatment cream Agent, seed treatment suspending agent, seed treatment liquor, seed treatment dispersible pulvis, seed treatment microcapsule suspending agent, seed treatment Gel, suspoemulsion, milk particle agent, ultra-low volume suspending agent, ultra low volume liquids, dispersibility concentrating agents.
The bactericidal composition, wherein the content of fluorine azoles bacterium acyl azanol and cyflufenamid active constituent accounts for composition 1%-90%, preferably 5%-80%, more preferable 10%-80%, more preferable 15%-70%.
In bactericidal composition of the invention, the quality of fluorine azoles bacterium acyl azanol and cyflufenamid accounts for the bactericidal composition with weight Meter can also be, for example:
1%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%, 21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%, 40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%, 59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%, 78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%。
Bactericidal composition of the invention has very strong activity to various phytopathogens, and can draw to by phytopathogen The prevention and treatment of the plant disease risen play very strong preventive effect.
Cereal, veterinary antibiotics, pathogenic is true on ornamental plant and grapevine for preventing and treating for the bactericidal composition The purposes of bacterium and bacterium.
Plant of the bactericidal composition to following classifications on Cereal, veterinary antibiotics, ornamental plant and grapevine Disease fungus has remarkable activity:
Fungi Imperfecti such as Botrytis (Botrytis), Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), chain Lattice spore category (Alternaria), Pyricularia Sacc. (Pyricularia), false Cercosporalla (Pseudocercospora)；
Basidiomycetes class such as Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), Puccinia (Puccinia), Phakopsora (Phakopsora), Ustilago (Llstilaginalcs)；
Ascomycetes class such as Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Uncinula (Uncinula), mycosphaerella (Mycosphaerella))；
Oomycete class such as Phytophthora (Phytophthora), pythium (Pythium), Plasmopara (Plasmopara), frost Mould category germ (Peronospora), Pseudoperonospora (Pseudoperonospora), disk stalk mould (Bremialactucae).
Bactericidal composition of the invention be also effective against phytopathogenic bacterium and virus (such as confrontation Xanthomonas (Xanthomonasspp), pseudomonas (Pseudomonas spp), erwinia amylovora (Erwinia amylovora) And tobacco mosaic virus (TMV)).
The important plant of the present invention be wheat, barley, rye, oat, potato, rice, sorghum, beet, a kind of fruit, such as apple, pear, etc., Drupe, apple, pears, Lee, peach, almond, cherry, strawberry, trichoderma, blackberry, blueberry, cucumber, muskmelon, cabbage, grape, hot red pepper, green pepper, Watermelon, pumpkin, tobacco, citrus, tomato, banana, corn, asparagus, hare's-lettuce, common calla, cauliflower, cabbage, konjaku, rape, Celery, onion, green stem vegetable, eggplant, carrot, green onion, Chinese cabbage, cabbage, romaine lettuce, oilseed rape, Kidney bean, lens, Pea, soybean, nut, coffee, eggplant, sugarcane, tea, pepper, liana, hops, cotton, flax, hemp, jute, spinach Dish, onion, leaf mustard, olive, sunflower, castor oil are with plant, cocoa bean and ornamental plant.
Bactericidal composition of the invention is especially suitable for controlling Botrytis (Botrytis), Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), Pyricularia Sacc. (Pyricularia), false Cercosporalla (Pseudocercospora), Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), Puccinia (Puccinia), Phakopsora (Phakopsora), Ustilago (Llstilaginalcs), Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Uncinula (Uncinula), mycosphaerella (Mycosphaerella)), it is Phytophthora (Phytophthora), pythium (Pythium), single The mould category (Plasmopara) of axis, peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora), disk stalk Mould (Bremialactucae).
Bactericidal composition of the invention is particularly suitable for controlling following plant disease: grape grey mould, wheat leaf blight, peanut Early pinta, target, the late blight of potato, wheat powdery mildew, net blotch of barley, wheat glume blotch, cucumber downy mildew, Downy mildew of garpe, soybean rust, wax gourd downy mildew, wax gourd anthracnose, tomato late blight, leaf muld of tomato, mandarin tree shot hole, Mandarin tree anthracnose, scab of cucumber, cucumber blight dis-ease, pepper anthracnose, capsicum epidemic disease, black scurf of potato, grape white rot Disease, downy mildew of garpe, sponge gourd downy mildew, brown rust of wheat, early blight of tomato, uncinula necator, watermelon anthrax, banana tikka Disease, powdery mildew of cucumber, wheat avenge mould leaf blight, wheat scab, lawn snow mold, rice sheath blight disease, rice blast, rice False smut, powdery mildew of strawberry, leaf muld of tomato, cucumber target disease, cucumber gray leaf spot, potato target spot, ring rot of apple, Apple tree early blight, apple peels and cores, scab of apple, Spike-stalk Brown Spot of Grape, watermelon powdery mildew, watermelon are climing withered Disease, banana brown spot, banana freckle, sigatoka, take-all, jujube tree rust, the leaf blight of corn, corn stigma Disease, watermelon anthrax, watermelon grafting, rice green smut, target, tea tree anthracnose, graw mold of tomato, lawn are brown Pinta, coffee rust, wheat rust, sclerotinia sclerotiorum, scab of cucumber, pear scab, lichee anthracnose, grape anthrax Disease, rice green smut, maize head smut, head smut of sorghum, loose smut of barley, barley banded sclerotial blight, Penicillium italicum, mandarin orange Tangerine green mould, powdery mildew of strawberry, wheat leaf rust, gray mold of cucumber.
The bactericidal composition is used to protect the purposes of plant, plant propagation material and the plant organ then grown.
It causes a disease in the place prevention and treatment soil or cultivation medium that the bactericidal composition is prevented and treated needed for being applied to or saprophytic The purposes of harmful fungoid.
The bactericidal composition is used to handle seed the purposes of the phytopathogen invasion to protect seed from carrying.
The bactericidal composition is used to protect the purposes of stock.
The bactericidal composition is for protecting stock in storage period from fungi or the purposes of bacterial invasion.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by sterilization of the invention Composition act on plant pathogen and/or its environment or plant, plant propagation material and the plant organ then grown, In soil or cultivation medium, material or space.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention Bactericidal composition is applied to plant leaf surface.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention The plant organ that bactericidal composition is applied to plant propagation material and then grows.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention Bactericidal composition is applied to soil or cultivation medium.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method is included in cultivated plant and is invaded Before dye or the bactericidal composition is acted on into plant pathogen and/or its environment after infecting or plant, plant are numerous Grow material and the plant organ, soil or the cultivation medium, material or space that then grow in.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by sterilization of the invention Composition with agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instil, The methods of casting, injection, spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant In propagation material and the plant organ then grown, soil or cultivation medium, material or space.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by fluorine azoles bacterium acyl azanol It is administered simultaneously with cyflufenamid or sequential application.
Bactericidal composition of the invention plants the wide scopes such as such as Basidiomycetes, Ascomycetes, Oomycete and deuteromycetes Object pathogenic epiphyte has excellent activity.
Oomycete, including Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthorainfestans), phytophthora sojae kaufmann&gerdemann (Phytophthoramegasperma), foot rot of citrus bacterium (Phytophthoraparasitica), camphor tree phytophthora (Phytophthoracinnamomi) and phytophthora capsici (Phytophthoracapsici) disease；Careless rotten mould withered category (Pythium) such as level ground grass Pythium aphanidermatu (Pythiumaphanidermatum) disease；And Peronosporaceae (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola)；Peronospora (Peronospora) (including Peronosporatabacina (Peronosporatabacina) and Peronospora parasitic bacterium (Peronosporaparasitica))；Pseudoperonospora (Pseudoperonospora category) germ (including bacterium of downy mildew of cucumber (Pseudoperonosporacubensis) and disk stalk it is mould Bacterium germ (Bremialactucae), pythium (Pythium) such as scraping and printing unit (Pythiumaphanidermatum)； Plasmopara (Plasmopara).
Ascomycetes, including Alternaria (Alternaria) disease such as tomato early blight bacterium (Alternariasolani) and black spot of cabbage bacterium (Alternariabrassicae), ball seat Pseudomonas (Guignardia) are sick Evil such as black rot of grape bacterium (Guignardiabidwelli), Venturia (Venturia) disease such as apple black star bacteria (Venturiainaequalis), Septoria (Septoria) disease such as glume blight bacterium (Septorianodorum) and leaf Blight bacterium (Septoriatritici), powdery mildew such as Erysiphe (Erysiphe) (including wheat powdery mildew (Erysiphegraminis) and trailing plants Powdery Mildew (Erysiphepolygoni)), grape powdery mildew (Uncinulanecatur), cucumber powdery mildew's pathogen (Sphaerothecafuligena) and apple mildew bacterium (Podosphaeraleucotricha), wheat Phyllostachys pubescens (Pseudocercosporellaherpotrichoides) object Kind, grey mold Pseudomonas (Botrytis) species disease such as Botrytis cinerea germ (Botrytiscinerea), Monilinia fructicola (Moniliniafructicola) disease, sclerotium Pseudomonas (Sclerotinia) species disease such as Sclerotinia sclerotiorum (Sclerotiniasclerotiorum), Pyricularia oryzae (Magnaporthegrisea), grape branch-rot bacterium (Phomopsisviticola) disease, compacted shape Pseudomonas (Helminthosporium) species disease such as Exserohilum turcicum (Helminthosporiumtriticirepentis), reticulate pattern germ (Pyrenophorateres) species, anthrax disease is for example Black fruit bacterium (Glomerella) or colletotrichum (Colletotrichum category) disease (such as fine strain of millet anthrax bacteria (Colletotrichumgraminicola) and watermelon anthrax bacteria (Colletotrichumorbiculare))；Wheat total eclipse Germ (Gaeumannomycesgraminis);Podosphaera (Podosphaera)；Chain sclerotinia sclerotiorum belong (Monilinia)； Uncinula (Uncinula)；Mycosphaerella (Mycosphaerella).
Basidiomycetes, including the rest fungus disease as caused by Rust (Puccinia category) (such as Puccinia recondita (Pucciniarecondita), strip rust bacteria (Pucciniastriiformis), leaf rust (Pucciniahordei), puccinia graminis bacterium (Pucciniagraminis) and handle rest fungus (Pucciniaarachidis)), coffee rest fungus (Hemileiavastatrix) and Phakopsora pachyrhizi sydow (Phakopsorapachyrhizi)；Rhizoctonia (Rhizoctonia)；Ustilago (Llstilaginalcs).
Deuteromycetes, including Rhizoctonia (Rhizoctonia category) species (such as Rhizoctonia solani Kuhn (Rhizoctoniasolani) and redness of the skin or complexion hyphal cluster germ (Rhizoctoniaoryzae));Fusarium (Fusarium) disease, Such as F.graminearum schw (Fusariumgraminearum), beads Fusariumsp (Fusariummoniliforme), fusarium oxysporum (Fusariumoxysporum, fusarium moniliforme (Fusariumproliferatum), fusariun solani (Fusariumsolani);Verticillium dahliae (Verticilliumdahliae);Sclerotiumrolfsii (Sclerotiumrolfsii)；Cloud Line bacterium (Rynchosporiumsecalis);Black puckery germ (Cercosporidiumpersonatum), alternaria (Cercosporaarachidicola) and brown patch germ (Cercosporabeticola);Silver dollar pinta bacterium (Rutstroemiafloccosum)；Botrytis (Botrytis)；Pyricularia Sacc. (Pyricularia)；Helminthosporium (Helminthosporium)；Fusarium (Fusarium)；Septoria (Septoria)；Cercospora (Cercospora)；Chain Lattice spore category (Alternaria)；Pyricularia Sacc. (Pyricularia);False Cercosporalla (Pseudocercospora).
Bactericidal composition of the invention is especially effective to the plant pathogenic fungi of following type: Botrytis (Botrytis), Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), shell Needle spore category (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), Pyricularia Sacc. (Pyricularia), False Cercosporalla (Pseudocercospora), Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), handle rust Pseudomonas (Puccinia), Phakopsora (Phakopsora), Ustilago (Llstilaginalcs), Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Uncinula (Uncinula), mycosphaerella (Mycosphaerella)), Phytophthora (Phytophthora), pythium (Pythium), Plasmopara (Plasmopara), peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora), disk stalk mould (Bremialactucae).
The suitable crop plants of bactericidal composition of the invention specifically include that cereal crops, for example, wheat, barley, oat, Naked barley, triticale, rice, corn, sorghum and millet；Vine crop, such as Table Grape and vinifera；Field crop, example Such as rape (Canola), sunflower；Sugar beet, sugarcane, soybean, peanut (peanut), tobacco, clover, clover, Hu Zhi Son, clover and vetch；Pip fruit, such as apple, pears, crabapple, loquat, hawthorn and quince；Stone fruit, example Such as peach, cherry, plum, apricot, honey peach；Citrus fruit, such as lemon, bitter orange, orange, shaddock, mandarin orange (orange) and kumquat； Radicant and field crop (and their leaf), such as arithoke, common beet and sugar beet, carrot, cassava, life Ginger, ginseng, horseradish, parsnip, potato, little radish, turnip, sweet potato, turnip and Chinese yam；Bulb bearing plant, such as Garlic, leek, onion and verdant；Leaf vegetables plant, for example, mustard seed Chinese Ixeris (rocket salad), celery, celery, Chinese celery, witloof (thatch dish), Fennel, bile ingredients and leaf lettuce, parsley, red witloof (red witloof), rheum officinale, spinach and Swiss chard；Btassica (Koryo Dish) leaf vegetables, for example (,) it is broccoli, cauliflower (broccoli), Brussels sprouts, cabbage, Chinese cabbage, cauliflower, wild cabbage, collard, big Head dish, leaf mustard and green vegetables；Bean (succulence or without juice) such as lupin, Kidney bean) (including semen viciae fabae, kidney bean, dish Beans, Hua Dou, scarlet runner bean, snap beans, wide leaf vegetables beans), Kidney bean (including red bean, asparagus bean, cow gram, black cowpea, long bean, cowpea Beans, mung bean, cowpea, urd bean and speciality cowpea), semen viciae fabae, chick-pea, melon ear, sword bean, Lens culinaris and pea (including kidney bean, food Pod pea, field pea, pea, green pea, snow bean, sweet tea beans, pigeonpea and soybean)；Fruit vegetables, such as eggplant, ground-cheery, muskmelon Eggplant and capsicum (including bell pepper, capsicum, capsicum for cooking, pimiento, pimento；Small tomato and tomato)；Cucurbit class vegetables, such as Chocho (fruit), wax gourd, citron watermelon, cucumber, young cucumber, marrow squash (including cucurbit, lagenaria sicerariae), sponge gourd, gumbo, glue are bitter Melon, dioecism momordica root, balsam pear and Chinese cucumber, muskmelon, cucurbita pepo and winter squash and watermelon；Berries, such as blackberry, blueberry, the red fruit certain kind of berries, dew the certain kind of berries, The purplish blue certain kind of berries, blueberry, Cranberry, currant, the wild certain kind of berries, Loganberry, raspberry and strawberry；Set raw nut, such as almond, beech heavily fortified point Fruit, Brazil nut, butternut, cashew nut, Chinese chestnut, fibert (filbert), hickory nut, Queensland nut, pecan and English walnut；Tropical water Fruit and other crops, such as banana, plantain, mango, coconut, pawpaw, avocado, lichee, American aloe, coffee, cocoa, sugarcane, oil Palm fibre, sesame, rubber and fragrance；Fibre crops, such as cotton, flax and hemp；Sod grass (including season type and shitivi type turf Grass).
The suitable crop plants of preferred bactericidal composition of the invention generally include following kind of platymiscium: Cereal is (small Wheat, barley, rye, oat, rice, corn, sorghum and related type)；Beet (sugar beet and fodder beet)；The operatic circle, core Fruit and berry (apple, pears, Lee, peach, apricot, cherry, strawberry, raspberry and blackberry, blueberry)；Leguminous plant (Kidney bean class, hyacinth bean class, pea Class, big beans)；Oily section plant (rape, leaf mustard, opium poppy, olive, sunflower, coconut, castor-oil plant, cocoa bean, peanut)；It is melon Plant (pumpkin, cucumber, muskmelon)；Fiber-like plant (cotton, flax, hemp, jute)；Tangerine fruit (orange, lemon, grape, citrus)； Greengrocery (spinach, lettuce, asparagus, cabbage, carrot, onion, tomato, potato, pimiento)；Lauraceae (avocado, cortex cinnamomi, camphor tree Brain) or such as tobacco, nut, coffee, eggplant, sugarcane, tea, pepper, grape, banana and natural rubber plant etc plant, and Ornamental plant.
The particularly suitable crop plants of bactericidal composition of the invention include rice, cucumber, muskmelon, cabbage, grape, red Green pepper, green pepper, watermelon, pumpkin, tobacco, citrus, apple, tomato, banana, corn, asparagus, lettuce, common calla, cauliflower, the volume heart Dish, konjaku, rape, celery, japanese radish, tobacco, onion, green stem vegetable, tomato, eggplant, carrot, green onion, Chinese cabbage, balling Wild cabbage, potato, romaine lettuce, oilseed rape.
Bactericidal composition of the invention is particularly effective to following item: powdery mildew；Rust；Leaf spot；Early blight；Cereal On Septoria, Puccinia, powdery mildew, Pyrenophora；Layer rest fungus on soybean；Aecidium on coffee；On rose More born of the same parents' Rusts；Alternaric bacteria on potato, tomato and cucurbit；Sclerotinite on lawn, vegetables, sunflower and rape；Rattan Black rot, flourishing disease, powdery mildew, gray mold and withered rattan disease on this plant；Botrytis cinerea on fruit；Chain core on fruit Cup fungi and Penicillium.
Bactericidal composition of the invention is particularly suitable for controlling following plant disease: grape grey mould, wheat leaf blight, peanut Early pinta, target, the late blight of potato, wheat powdery mildew, net blotch of barley, wheat glume blotch, cucumber downy mildew, Downy mildew of garpe, soybean rust, wax gourd downy mildew, wax gourd anthracnose, tomato late blight, leaf muld of tomato, mandarin tree shot hole, Mandarin tree anthracnose, scab of cucumber, cucumber blight dis-ease, pepper anthracnose, capsicum epidemic disease, black scurf of potato, grape white rot Disease, downy mildew of garpe, sponge gourd downy mildew, brown rust of wheat, early blight of tomato, uncinula necator, watermelon anthrax, banana tikka Disease, powdery mildew of cucumber, wheat avenge mould leaf blight, wheat scab, lawn snow mold, rice sheath blight disease, rice blast, rice False smut, powdery mildew of strawberry, leaf muld of tomato, cucumber target disease, cucumber gray leaf spot, potato target spot, ring rot of apple, Apple tree early blight, apple peels and cores, scab of apple, Spike-stalk Brown Spot of Grape, watermelon powdery mildew, watermelon are climing withered Disease, banana brown spot, banana freckle, sigatoka, take-all, jujube tree rust, the leaf blight of corn, corn stigma Disease, watermelon anthrax, watermelon grafting, rice green smut, target, tea tree anthracnose, graw mold of tomato, lawn are brown Pinta, coffee rust, wheat rust, sclerotinia sclerotiorum, scab of cucumber, pear scab, lichee anthracnose, grape anthrax Disease, rice green smut, maize head smut, head smut of sorghum, loose smut of wheat, barley banded sclerotial blight, Penicillium italicum, mandarin orange Tangerine green mould, powdery mildew of strawberry, wheat leaf rust, gray mold of cucumber.
Bactericidal composition of the invention can be used as foliage fungicide in crop protection, can also be used as fungicide for mixing Plant and be used as soil fungicides.Bactericidal composition of the invention is for protecting plant, plant propagation material and the plant then grown The purposes of sundries official.Bactericidal composition of the invention is for handling seed to protect seed from the plant pathogenic fungi of carrying The purposes of invasion.It causes a disease in the place prevention and treatment soil or cultivation medium that bactericidal composition of the invention is prevented and treated needed for being applied to or rotten The purposes of raw phytopathogen.
Bactericidal composition of the invention can also particularly effectively prevent or control kind of biography or a soil-borne disease.What kind biography or soil passed The example of aquacultural fungal pathogen includes Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), grey Portugal Grape spore (Botrytis cinerea, Cercospora (Cercospora spp., ergot (Clavicepspurpurea), standing grain rotation Spore chamber bacterium (Cochliobolussativus), Colletotrichum (colletotrichum spp., Epicoccum (Epicoccum Spp., F.graminearum schw (Fusariumgraminearum), beads Fusariumsp (Fusariummoniliforme), fusarium oxysporum (Fusariumoxysporum, fusarium moniliforme (Fusariumproliferatum), fusariun solani (Fusariumsolani), glue chain spore (Fusariumsubglitinans) is tieed up, Helminthosporium (Helminthosporiumspp), the withered bacterium of mould leaf (Gerlachia nivalis), Penicillium (Pencilliumspp), stem are avenged The mould category (Phoma spp.) of point, wheat nuclear cavity bacteria (Pyrenophoragraminea), rice blast Pyricularia Sacc. (Pyriculariaoryzae), Rhizoctonia solani Kuhn (Rhizoctoniasolani), Rhizoctonia cerealis (Rhizoctoniacerealis), Sclerotinia (Sclerotinia spp.), Septoria (Septoria spp.), silk axis Smut (Sphacelothecareilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula Incarnate), hidden smut (Urocystisocculta), Ustilago (Ustilago spp.), Verticillium (Verticillium spp.), Phytophthora (Phytophthora), the rotten mould withered category (Pythium) of grass, Peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora).
The bactericidal composition is used to protect the purposes of stock.
The bactericidal composition is for protecting stock in storage period from fungi or the purposes of bacterial invasion.
Bactericidal composition of the invention apply also for prevent or control harvest after and storage disease.According to the present invention, it harvests Afterwards can be for example by caused by following fungi with the disease of storage period: Colletotrichum kind, such as banana pierce disk spore (Colletotrichum musae), Colletotrichum gloeosporiodes (Colletotrichum gloeosporioides), capsicum thorn Disk spore (Colletotrichum coccodes)；Fusarium kind, such as fusarium semitectum (Fusarium semitectum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum)；Verticillium dahliae belongs to kind, such as cocoa verticillium sp (Verticillium theobromae)；It is black The mould category kind of spore；Botrytis kind, such as the pathogen of Botrytis cinerea (Botrytis cinerea)；Geotrichum, such as geotrichum candidum (Geotrichum candidum)；Phomopsis kind, Natal Phomopsis (Phomopsis natalensis)；Color two Spore belongs to kind, such as two spore of citrus color (Diplodia citri)；Alternaria kind, such as Altemaria citri (Alternaria Citri), mutually every Alternariaspp (Alternaria alternata)；Phytophthora kind, such as phytophthora brown rot of citrus (Phytophthora citrophthora), strawberry phytophthora (Phytophthora fragariae), Phytophthora cactorum (Phytophthora cactorum), Phytophthora nicotianae (Phytophthora parasitica);Septoria (Septoria ), such as Septoria depressa spp.；Mucor (Mucor spp.), such as pyriform Mucor (Mucor piriformis)；Chain sclerotinia sclerotiorum belong (Monilinia spp.), such as the raw chain sclerotinia sclerotiorum (Monilinia of fruit Fructigena), drupe chain sclerotinia sclerotiorum (Monilinia laxa)；Venturia (Venturia spp.), such as the black star of apple Bacterium (Venturia inaequalis), Pear scab (Venturia pyrina)；Rhizopus (Rhizopus spp.), such as Rhizopus stolonifer (Rhizopus stolonifer), Rhizopus oryzae (Rhizopus oryzae)；It is small from shell category (Glomerella Spp.), such as enclose small from shell (Glomerella cingulata)；Sclerotinia (Sclerotinia spp.), such as fruit are raw Sclerotinite (Sclerotinia fruiticola)；Long beak shell category (Ceratocystis spp.), such as unusual long beak shell (Ceratocystis paradoxa)；Penicillium (Penicillium spp.), such as penicillium funiculosum (Penicillium Funiculosum), penicillium expansum (Penicillium expansum), Penicillium digitatum (Penicillium digitatum), Italian mould (Penicillium italicum)；The long spore category of disk (Gloeosporium spp.), such as the long spore of white disk (Gloeosporium album), Gloeosporium perennans, the raw long spore (Gloeosporium of disk of fruit fructigenum),Gloeosporium singulata；Shell snake spore category (Phlyctaena spp.), such as Phlyctaena vagabunda；Cylindrocarpon (Cylindrocarpon spp.), such as apple column spore (Cylindrocarpon mali)；It crawls handle Mould category (Stemphyllium spp.), such as day lily stemphylium botryosum (Stemphyllium vesicarium)；Star check shell spore Belong to (Phacydiopycnis spp.), such as Phacydiopycnis malirum；Thiclaviopsis (Thielaviopsis Spp.), such as singular root string strain is mould (Thielaviopsis paradoxy)；Aspergillus (Aspergillus spp.) is such as black Aspergillus (Aspergillus niger), Aspergillus carbonerius (Aspergillus carbonarius);Nectria (Nectria ), such as the red shell bacterium of dry cancer clump (Nectria galligena) spp.；Stockless Peziza (Pezicula spp.).
Bactericidal composition of the invention can also be used to prevent and treat fruit vegetables storing period disease, and obtain unexpected collaboration The effect of synergy.Such as the fruit rot as caused by following pathogen:
Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthorainfestans), phytophthora sojae kaufmann&gerdemann (Phytophthoramegasperma), foot rot of citrus bacterium (Phytophthoraparasitica)；
Peronospora (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola)；
Pythium (Pythium) such as scraping and printing unit (Pythiumaphanidermatum).
After bactericidal composition according to the present invention can also being applied when plant or plant part grow to protect harvest Stock.
Bactericidal composition of the invention is especially effective to prevention and treatment following plants disease:
Alternaria in fruits and vegetables；
Ascochyta in legume crop；
Gray botrytis (grey mold) in strawberry, tomato, sunflower, legume crop, vegetables and grape；
Peanut Cercospora bacteria on peanut；
Peanut tail spore in peanut；
Standing grain rotation spore in cereal is mould；
Hair disc spore category in legume crop；
Erysiphe in cereal；
Anthrax-bacilus species on legume crop；
Two spore powdery mildews and Siberian cocklebur monofilament shell bacterium in cucurbit；
Fusarium in cereal and corn；
Gaeumannomyce in cereal and lawn；
Helminthosporium in corn, rice and potato；
Coffee rust on coffee；
Micro- tubercle Pseudomonas in wheat and rye；
Coffee rust on wheat and rye；
Phakopsora in soybean；
Puccinia in cereal, broad leaf crop and perennial plant；
False Cercosporalla in cereal；
The more born of the same parents rest fungus of short point in rose；
Magnaporthe grisea on rice；
Swan rubber column gel column on barley is every spore；
Caulococcus in fruit；
Pyrenophora in barley；
Pyricularia oryzae in rice；
Rhizoctonia in cotton, soybean, cereal, corn, potato, rice and lawn；
Mouth spore category in barley and rye；
Sclerotinia in lawn, lettuce, vegetables and oily seed rape；
Septoria in cereal, soybean and vegetables；
Powdery mildew species in fruit；
Head smut bacterium in corn；
Tilletia in cereal；
Uncinula necator snag shell in liana is mould, skin committee's Richter scale Guignardia and grape Phomopsis；
A smut is hidden in rye；
Ustilago in cereal and corn；
Venturia in fruit；
The mould category of beads on fruit；
Penicillium on citrus and apple.
Bactericidal composition of the invention is particularly effectively fought harvest after following harvest after and storage period disease disease: such as ash Grape spore, banana anthracnose, curved spore, F.semitectum, geotrichum candidum, peach brown rot mould, the raw chain sclerotinia sclerotiorum of fruit, drupe chain sclerotinia sclerotiorum, Pyriform Mucor, Italian mould, from raw mould.
Bactericidal composition of the invention can handle all plants and plant part." plant " refers to all plants and plant Population, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant Cultivate the protection of human rights benefit).Cultivated plant and plant variety can be the plant obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique methods, such as use dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Part and organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, running roots Plant part is also belonged to seed.
Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use In breeding the latter and vegetable matter such as cuttage item or stem tuber (such as potato).Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.To The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by sterilization of the invention Composition act on plant pathogen and/or its environment or plant, plant propagation material and the plant organ then grown, In soil or cultivation medium, material or space.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention Bactericidal composition is applied to plant leaf surface.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention The plant organ that bactericidal composition is applied to plant propagation material and then grows.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes will be of the present invention Bactericidal composition is applied to soil or cultivation medium.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method is included in cultivated plant and is invaded Before dye or the bactericidal composition is acted on into plant pathogen and/or its environment after infecting or plant, plant are numerous Grow material and the plant organ, soil or the cultivation medium, material or space that then grow in.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by sterilization of the invention Composition with agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instil, The methods of casting, injection, spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant Partially, in plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by fluorine azoles bacterium acyl azanol It is administered simultaneously with cyflufenamid or sequential application.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and the bactericidal composition is acted on Plant propagation material and the plant organ then grown.
Currently preferred plant propagation material is seed.Bactericidal composition of the invention is also particularly suitable for processing seed.
Crop damage caused by most harmful fungoid is due to sending out during storage or after sowing and in plant Caused by the infringement of seed during bud or after germination.Due to the root and branch of growth period plant are especially sensitive and even if Small damage can result in the death of plant.Another aspect of the present invention provides a kind of method for protecting seed and germinating plants, should Method make after planting or after plant germination without additional application crop protection agents or the additional application crop guarantor at least significant ground Protect agent.On the other hand, optimize the amount of used reactive compound, using bactericidal composition of the invention farthest to mention For seed and germinating plants protection against the invasion of plant pathogenic fungi, and plant itself not will receive and use activity The damage of compound.
Therefore, the present invention is also in particular to by handling seed with bactericidal composition of the invention to protect seed and hair The method that bud plant invades from plant pathogenic fungi.The invention further relates to bactericidal compositions according to the present invention in processing kind Son is to protect seed and germinating plants from the purposes of plant pathogenic fungi.
Harm budding after plant plant pathogenic fungi control mainly by using crop protection agents processing soil and The aerial part of plant carries out.Healthy issuable influence in view of crop protection agents on environment and humans and animals, It is therefore desirable to reduce the amount of application of reactive compound to the greatest extent.
Bactericidal composition according to the present invention is suitable for protection in agricultural, in greenhouse, in forestry or gardening-or vinegrowing The seed of any plant variety of kind application.Particularly, the seed form used is cereal (such as wheat, barley, rye, black Wheat, millet, oat), soybean, sorghum, pea, lens, corn, cotton, soybean, rice, potato, sunflower, Kidney bean, coffee Coffee, beet, peanut, rape, olive, cocoa, sugarcane, tobacco, vegetables (such as tomato, cucumber, onion and lettuce), turfgrass and Decoration uses plant.The processing of the seed of cereal and greengrocery is vital.
Active constituent fluorine azoles bacterium acyl azanol and cyflufenamid in bactericidal composition of the invention is individually or with suitable system Dosage form formula is applied to seed.It is preferably handled in the state of substantially stabilized so that processing will not cause any damage to seed Evil.In general, can any point-in-time between picking and sowing carry out processing seed.Commonly used seed is separated from plant And it is isolated from cob, shell, stem, epidermis, hair or pulp.Therefore, it is possible to use for example, extremely by picking, cleaning and drying Water content is lower than 15% seed.Alternatively, it is also possible to for example with water process after using drying, the kind then dried once more Son.
The medicament of the method for seed treatment, such as can there are, diluent liquid or solid-like does not have to dilution directly general Seed is immersed in liquid condition solution the method for making medicament be impregnated with seed, is blended in solid chemicals or liquid preparation and seed Together, being coated processing makes the method for the surface of the seed attachment medicament, the methods of sprinkling near seed while plantation.
Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed Part.Plant part, plant organ and plant also can benefit from by the way that bactericidal composition is applied to plant propagation material institute The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as Material, its own can apply (or processing) with bactericidal composition；To by processed plant part and processed plant Plant, other plant parts and the other plant organs that organ generates also can benefit from by applying bactericidal composition With.
It causes a disease in the place prevention and treatment soil or cultivation medium that bactericidal composition of the invention is prevented and treated needed for being applied to or saprophytic Fungi and bacterium purposes.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and the bactericidal composition is acted on Soil or cultivation medium.
Under normal circumstances, soil germ can generate a large amount of thallus, as long as condition develops favorably and host pathogen growth Be again it is susceptible, germ mass propagation and can infect host, and in the presence of susceptible host, these germs can enter The lasting pathogenic phase, with the continuous cropping of crop mass propagation spread, but later nutrient be depleted or edaphic condition such as temperature, When humidity etc. is unfavorable to germ, germ can enter dormant period again.In the absence of susceptible host, soil-borne disease bacterium is in the soil It can survive, in addition to soil germ has extensive host range, moreover it is possible to be deposited on the root surface of non-host or the broken branches and fallen leaves It is living, it is undivided with it with saprophytic competitiveness.But different germs be it is discrepant, as sickle-like bacteria almost may be used in the soil It is survived with indefinite duration.
Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as: soil, water etc., Specific raw material can be used for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Sawdust, bark etc..
The method that medicament is applied into soil, such as liquid preparation is diluted in water or is not diluted and is directly applied to plant The methods of in the root of body or the rice seedling bed of seedling, granule is disseminated to the side in the root of plant or the rice seedling bed of seedling Method has the method that pulvis, water dispersible granules etc. are sprayed in soil prior to seeding and are integrally mixed with soil, before sowing or plants Implantation hole will be sprayed on before kind of plant after pulvis, water-dispersible grain dilution agent, in seed furrow, carrying out method of sowing etc..
Processing method according to the present invention can also be used for the invasion for protecting stock from fungi and microorganism.According to this hair It is bright, it is understood as referring to term " stock " to have originated from natural life cycle and it is desirable that the plant of long-term preservation or animality is come The natural materials in source and its processed form.Stock, such as plant of plant origin or part thereof, as stem, leaf, stem tuber, Seed, fruit or seed, can be with the state of fresh harvesting or with form processing such as (pre-) dry, wetting, crushing, grinding, pressure System or baking are protected.It is also possible to timber, thick timber form such as construction timber, electric pole and fence；Or final product form, such as by Furniture or article made of timber.The stock of animal origin is animal skin, leather, hair, hair etc..Composition according to the present invention It can prevent the invasion such as burn into colour fading or mouldy of the fungi or bacterium of storage period.It is preferred that " stock " is understood as referring to plant The natural materials in object source and its form processing, more preferable fruit and its form processing, as the operatic circle, drupe, soft fruit and Citrus fruit and its form processing.
The present invention provides a kind of method for preventing and treating or preventing plant pathogenic fungi and infect cultivated plant, and this method includes that incite somebody to action this The bactericidal composition of invention with agronomy is effective and the amount of application of basic plant-less toxicity with seed treatment, foliage applying, stem application, Be impregnated with, instil, being poured, spraying, being sprayed, dusting, the methods of distribution or smoke are applied to plant pathogen and/or its environment, or In person plant, plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.
Bactericidal composition of the invention can be applied by different processing methods, these methods for example:
Liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；
Dusting mixes particle or powder in the soil, sprays in the surrounding plants, and the tree injection or smearing the case where Under；
Cladding or film coated are carried out to the seed of plant.
When for postharvest fruit and vegetable anti-corrosive fresh-keeping, 200-2000 times of liquid is usually diluted with water, is leached after soaking fruit.
The present invention provides a kind of prevention and treatment or prevention plant pathogenic fungi infects the method for cultivated plant, can be treatment, in advance Anti- or method of eradication.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, and this method includes by fluorine azoles bacterium acyl azanol It is administered simultaneously with cyflufenamid or sequential application.
A method of prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, before infecting or can be infected in plant The bactericidal composition is acted on into plant pathogen and/or its environment or plant, plant propagation material and then later In plant organ, soil or cultivation medium, material or the space grown.
There may be super plus (" collaboration ") effects for processing according to the present invention.For example, the sterilization group used according to the present invention It closes the rate of application of object and/or widens its field of activity and/or increase its activity, it is possible to obtain following effect: better plant Growth increases the tolerance of high temperature or low temperature, increases the tolerance of arid or water or soil salt content, blooming performance mentions It is high, it is easier to harvest, the maturation of quickening, higher yield rate, bigger fruit, higher plant height, the color of leaf is more It is green, it blooms earlier, the quality or nutritive value of the product of harvest are higher, and sugared concentration is higher in fruit, the storage of the product of harvest More preferably, these benefits have been more than the effect actually estimated for stability and/or processability.
Processing method of the invention can also be used in handle propagation material such as stem tuber or rhizome, and can be used for handling seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention Processing method can also be used for the aerial part of processing plant such as dry, stem or stalk, leaf, flower and fruit in relation to plant.
The dosage of bactericidal composition of the present invention depends on various factors, compound as used；The object of processing, such as Plant, soil or seed；The type of processing, such as be sprayed, dust or dress seed；The purpose of processing, such as prevention or treatment；It is intended to prevent The type or administration time for the fungi controlled.
Generally for leaf portion processing: 5-2000g/ha, preferably 10-1000g/ha, more preferable 20-300g/ha；For irrigating Or be added dropwise for application, the dosage can also even reduce, especially when applying inert base such as asbestos or pearl rock；
For seed treatment: 0.1-2500g/100kg seed, preferably 3-1000g/100kg seed, more preferable 5-500g/100kg Seed, even more preferably 5-250g/100kg seed, even more preferably 5-100g/100kg seed, more preferable 5-50g/ 100kg seed
For soil or processing used for ponds: 0.1-10000g/ha, preferably 1-5000g/ha.
It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.
Fluorine azoles bacterium acyl azanol and cyflufenamid combination/combined administration of the invention.Including separating, sequentially or simultaneously applying fluorine Azoles bacterium acyl azanol and cyflufenamid.Preferably, the fluorine azoles bacterium acyl azanol and cyflufenamid group are combined into comprising fluorine azoles bacterium acyl azanol With the form of the composition of cyflufenamid.
Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.
Bactericidal composition of the invention can be used with any conventionally form, including aerosol, capsule suspension, harlization are dense Contracting agent, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, instant solution, sprayable pulvis, emulsifiable concentrating agents, oil-in-water type Emulsion, water-in-oil emulsion, bulky grain agent, fine granule, the dispersible powder of oil, the miscible flowable concentrating agents of oil, oil can mix Solution body, foaming agent, paste, suspension concentrating agents, solvable concentrating agents, suspending agent, seed coat agent, wettable powder, water dispersible granules, Soluble powder, coated granule, squeezes out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, super-low capacity at microcapsule suspending agent Measure liquor, suspoemulsion, the cold atomization preparation of ultra-low volume, super-low capacity calorimetric atomization preparation, double pack (twin pack), at seed Manage dry powder doses, seed treatment emulsion, seed treatment suspending agent, seed treatment liquor, seed treatment dispersible pulvis, seed treatment Microcapsule suspending agent, seed treatment gel, suspoemulsion, milk particle agent, ultra-low volume suspending agent, ultra low volume liquids, dispersibility are dense Contracting agent.
It is living comprising fluorine azoles bacterium acyl azanol and cyflufenamid, filler and/or surface in bactericidal composition of the present invention Property agent.
Bactericidal composition of the present invention, wherein the content of fluorine azoles bacterium acyl azanol and cyflufenamid accounts for bactericidal composition 1%-90%。
Bactericidal composition of the present invention, wherein the content of fluorine azoles bacterium acyl azanol and cyflufenamid accounts for bactericidal composition 5%-80%。
The bactericidal composition, wherein the content of fluorine azoles bacterium acyl azanol and cyflufenamid accounts for the 10%- of bactericidal composition 80%。
The bactericidal composition, wherein the content of fluorine azoles bacterium acyl azanol and cyflufenamid accounts for the 15%- of bactericidal composition 70%。
According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.
The nonactive medium that can be used in the present invention can be used as solid matchmaker either solid is also possible to liquid What dielectric material used has for example: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut Shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, the residue etc. after extracting plant essence), paper It opens, sawdust, crushes synthesized polymer body, the clay class (such as kaolin, bentonite, acid china clay etc.), talcum powder of synthetic resin etc. Class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class (float stone, Lv Po Thread stone and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride etc.), chlorination such as plastics medium Chemical fertilizer, the soil of the inorganic mineral powder of potassium, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, ammonium chloride etc. Fertilizer, these substances can be used alone or two or more is mixed.
It can be used as can selecting in llowing group of materials for liquid media materials'use, such as water, alcohol type (such as first Alcohol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng), ethers (such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene, Mineral oil etc.), aromatic hydrocarbons class (such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro rouge Fat hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amides, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal oil etc..
To make the emulsification of effective component compound, dispersion and/or wetting, surfactant can be used for example can be with Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine combination.
To make effective component compound dispersion stability, attachment and/or combination, such as xanthan gum, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid (such as cephalin and lecithin) with And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.
The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.
Organic silicon defoamer can be used as defoaming agent.
The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff: alizarin dyes, azo dyes and metallized phthalocyanine dye；And microelement, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.
Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.
The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention. The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing Thick dose, water and other processing aids.
These compositions not only include can or dusting equipment such as spraying by suitable equipment be suitable for immediately it is to be processed Object, but also including needing the concentration commercial composition being diluted before being applied to object.
Fluorine-containing azoles bacterium acyl azanol of the invention and cyflufenamid can also be applied with other active ingredient combinations, such as Expand activity profile or prevents from forming resistance.Other active constituents such as fungicide, bactericide, attractant, insecticidal Agent, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..
Active constituent in bactericidal composition of the present invention can also with itself or with its dosage form with it is known antifungal Agent, bactericide, acaricide, nematicide or insecticide are used in mixed way, for example to widen its action spectrum or prevent resistance It generates.
Other active constituents for example coumoxystrobin, fluorine bacterium mite ester, amine benzene pyrrole bacterium ketone, isopropyl metsulfovax, chlorine nalidixic bacterium ester, Mandipropamid, mepanipyrim, SSF 126, orysastrobin, pyraclostrobin, trifloxystrobin, oxygen ring azoles, bromuconazole, Cyproconazole, benzene Ether methyl cyclic-azole, olefin conversion, olefin conversion-M, epoxiconazole, benzoxazole, Fluquinconazole, Flusilazole, Flutriafol, hexaconazole, imazalil, kind Bacterium azoles, metconazole dislike imidazoles, pefurazoate, penconazole, Prochloraz, propiconazole, prothioconazoles, simeconazoles, Tebuconazole, ring fluorine bacterium Amine, triazolone, Triadimenol, fluorine bacterium azoles, triticonazole, diclobutrazol, etaconazole, furconazole, azoles oxolinic acide, fenpiclonil cough up bacterium Nitrile, cyprodinil, mepanipyrim, pyrimethanil, dodemorph, butadiene morpholine, tridemorph, fenpropidin, volution bacterium amine are anti-fall Ester, cycocel, ethephon (CEPHA),2-(chloroethyl) phosphonic acid, Bravo, Famoxate, Fenamidone, benomyl, carbendazim, probenazole, thiophanate, methyl support Cloth saliva, iprodione, procymidone, vinclozolin, bitertanol, chlorofluorobenzene ancymidol, metalaxyl, Metalaxyl-M (metalaxyl- M), ofurace, Wakil, different rice blast net (IBP), Isoprothiolane, carboxin, fenfuram, flutolanil, furan pyrazoles spirit, mebenil, Oxycarboxin, thiophene fluorine bacterium amine, bupirimate, ethirimol, diethofencarb, quinoxyfen, dimethomorph, zarilamid, tetrachlorobenzene Phthalein, pyroquilon, tricyclazole, fenhexamid, polyoxin, Pencycuron, cyazofamid, zoxamide, blasticidin S, spring thunder are mould Element, cymoxanil, Propamocarb, prothiocarb, fluazinam, Fentin chloride, oxolinic acid, octhilinone, captan, Bravo, cupric octoate, sulphur Sour copper, Kocide SD, dichlofluanid, dithianon, folpet, Guanoctine, iminoctadine, Mancozeb, maneb, Dai Sen Connection, thiram, zineb, iprovalicarb, fluorine mepanipyrim, Guardian, flusulfamide, methasulfocarb, Silthiopham, withered grass gemma Bar, dichloropropylene, iprovalicarb, polyoxin, Boscalid, hexaconazole, pyrrole metsulfovax, paclobutrazol, avermectin, efficient chlorine Cyano chrysanthemate, Amitraz, benomyl, Bifenazate, bifenthrin, brofenxalerate, bromophos, fenisobromolate, Buprofezin, pyridaben, Thiophene mite amine, chinomethionat, Neotran, Spanon, chlorfenapyr, Qikron, chlorfenizon, chlorfensulphide, chlopyrifos, cypermethrin, butyl ether Urea, basudin, dicofol, elgetol, Fenpropathrin, fenpyroximate, fenvalerate, Fipronil, fluacrypyrim, flucycloxuron, Flucythrinate, flufenoxuron, flumethrin, thiophene mite copper, ivermectin；Lufenuron, Methomyl, bromomethane, MTMC, speed are gone out Phosphorus, milbemectin, oxamyl, parathion, Profenofos, pyridaben, pyridaphethione, rotenone, Envidor, Spiromesifen, fluorine worm Amine, tebufenpyrad, Hostathion, avermectin, emaricin, methylamino first dimension rhzomorph benzoate, ivermectin, Kocide SD, Kasugarnycin, cue-lure, eugenol, avermectin, Acetamiprid, chlorfenapyr, chlopyrifos, chlorpyrifos-methyl, Actellic, ring worm Hydrazides, cyhalothrin；Clothianidin, imidacloprid；Indoxacarb, Isoprothiolane, ivermectin, lufenuron, lythidathion, methoxyfenozide, Milbemectin, Nitenpyram, Nithiazine, oxamyl, parathion；Parathion-methyl, penfluron, pentachlorophenol, phenothrin, Phenthoate dimephenthoate cidial, thimet；Profenofos, the third Flumethrin, pymetrozine, pyridaben, pyridaphethione, pleocidin, Spiromesifen, fluorine worm Amine, Diacloden, thiocyclam, thiodicarb, dimehypo, Tolfenpyrad, triflumuron, avermectin, benomyl, pyridaben, carbofuran, Chlopyrifos, metham-sodium.
Reactive compound fluorine azoles bacterium acyl azanol and cyflufenamid can be administered simultaneously, or apply respectively or sequential application, separate Sequence when application is on the result of prevention and treatment usually without influence.
Bactericidal composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition Ingredient can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with Based on dosage form provided by the invention.
For bactericidal composition of the invention in the case where reducing reactive compound application total amount, having to harmful fungoid improves activity (synergy).And bactericidal composition of the invention shows that the bacterium of tolerance also has excellent kill to existing fungicide Bacterium effect.
A kind of bactericidal composition of the present invention, the composition is by combining fluorine azoles bacterium acyl azanol and cyflufenamid, so that obtaining To composition there is gain effect in control efficiency, and expanded fungicidal spectrum, played the multi-purpose effect of a medicine, effectively Slow down or germ is avoided to develop drug resistance.Astoundingly, the bactericidal activity of bactericidal composition of the invention is than each activation The active adduction for closing object is considerably higher, and there are unpredictable, necessary being synergistic effects, rather than just active increasing It mends.
Bactericidal composition of the present invention is also surprising with plant physiology effect other than antifungal synergistic effect Effect.The plant physiology includes:
Abiotic stress resistance, including after temperature-resistant, drought tolerance and drought stress recovery, water application efficiency is (with water consumption Reduce related), flood resistance, ozone stress and UV resistance, to chemical substance such as heavy metal classes, salt, insecticide (safety Agent) etc. resistance.
Biotic resistance, including increased fungus resistant and the increased resistance for being directed to nematode, virus and bacterium.
Increased plant vigor, including plant health/plant quality and seed vitality, the appearance of reduction toppled over, improved, The increased afforestation effect for restoring, improving and improved photosynthetic efficiency.
Bactericidal composition of the invention at least expands the sphere of action of fluorine azoles bacterium acyl azanol and cyflufenamid both ways. Firstly, reducing the amount of application of fluorine azoles bacterium acyl azanol and cyflufenamid, while the still same holding of its effect is good.Secondly, even if Single compound becomes completely ineffective under low amount of application, and the combination of fluorine azoles bacterium acyl azanol and cyflufenamid still obtains height Phytopathogen control effect.On the one hand the phytopathogen spectrum that can be prevented and treated is expanded, is on the other hand improved using peace Quan Xing.
On the other hand, bactericidal composition of the invention can also effectively antagonize various can lead to microorganism infection in animal Microbial species.E.g. cause the microorganism of aspergillosis (Aspergillosis), such as aspergillus fumigatus (Aspergillusfumigatus), aspergillus flavus (A.fIavus), Aspergillus terreus (A.terrus), structure nest aspergillus (A.nidulans) With aspergillus niger (A.niger)；Cause blastomycosis (Blastomycosis), such as Blastomyces dermatitidis (Blastomycesdermatitidis)；Cause candidiasis (Candidiasis), such as Candida albicans (Candidaalbicans), Candida glabrata (C.glabrata), Candida tropicalis (C.tropicalis), nearly smooth beads Bacterium (C.parapsilosis), candida krusei (C.krusei) and Candida lusitaniae (C.1usitaniae);Cause ball Pityrosporion ovale disease (Coccidioidomycosis), such as posadasis spheriforme (Coccidioides immitis);Cause hidden ball Bacterium disease (Cryptococcosis), such as neogenesis cryptococcus (Cryptococcus neoformans);Cause histoplasma capsulatum Sick (Histoplasmosis's), for example capsule tissue's spore slurry bacterium (Histoplasma capsulatum) and cause zygomycete Sick (Zygomycosis's), such as absidia corymbifera (Absidia corymbifera), Rhizomucor pusillus (Rhizomucor ) and Rhizopus arrhizus (Rhizopus arrhizus) pusillus.There are also such as Fusariums (Fusarium Spp), such as sharp spore Fusarium (Fusarium oxysporum) and fusariun solani (Fusarium solani) and the more spore category (Scedosporium of match ), Spp for example more spores (Scedosporium apiospermum) are matched at tip and more spore (Scedosporium are matched in many births Prolificans), Microsporon (Microsporum Spp), hair moss Pseudomonas (Trichophyton Spp), epidermis moss bacterium Belong to (Epidermophyton Spp), mucor (Mucor Spp), Sporothrix (Sporothorix Spp), Saksenaea (Phialophora Spp), branch spore category (Cladosporium Spp), the mould category (Petriellidium spp) of Petrie, secondary ball Spore Pseudomonas (Paracoccidioides Spp) and tissue spore slurry Pseudomonas (Histoplasma Spp).
Specific embodiment
Below with reference to embodiment, the invention will be further described:
Example of formulations
+ 6% cyflufenamid wettable powder of 1 18% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 18%
Cyflufenamid 6%
Lauryl sodium sulfate 3%
Sodium lignin sulfonate 5%
Height silicic acid 5%
Kaolin Complement to 100%
By active constituent, various auxiliary agents and filler etc. by formula proportional components mix, after ultra-fine pulverizer disintegrating to get To+6% cyflufenamid wettable powder of 18% fluorine azoles bacterium acyl azanol.
+ 10% cyflufenamid missible oil of 2 10% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 10%
Cyflufenamid 10%
Octyl phenolic group polyglycol ether 3%
Castor oil polyglycol ether (36mol ethylene oxide) 5%
Calcium dodecyl benzene sulfonate 3%
Methyl oleate Complement to 100%
Mentioned component is proportionally prepared, uniform phase is uniformly mixing to obtain.
+ 5.5% cyflufenamid water dispersible granules of 3 14.5% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 14.5%
Cyflufenamid 5.5%
MODIFIED LIGNOSULPHONATE 3%
Lauryl sodium sulfate 2%
Urea 1%
Kaolin Complement to 100%
Fluorine azoles bacterium acyl azanol, cyflufenamid active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed in the ratio of formula It closes uniformly, by air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.It is obtained after dry screening + 5.5% cyflufenamid water dispersible granules of 14.5% fluorine azoles bacterium acyl azanol.
+ 10% cyflufenamid suspoemulsion of 4 18% fluorine azoles bacterium acyl azanol of embodiment
Oily phase:
Cyflufenamid 10%
SOLVESSOTM200 10%
Ethoxylated castor oil 5%
Water phase:
Fluorine azoles bacterium acyl azanol 18%
Polyoxyethylene tri-styryl phenol (10-20mol ethylene oxide) 2%
Water Complement to 100%
Cyflufenamid is dissolved in SOLVESSOTMIn 200, ethoxylated castor oil is added and obtains oily phase；According to formula fluorine azoles bacterium Fluorine is obtained after acyl azanol, polyoxyethylene tri-styryl phenol (10-20mol ethylene oxide), water is ground and/or high speed shear The aqueous suspension agent of azoles bacterium acyl azanol；The oil phase is added to the aqueous phase under stiring obtains suspoemulsion.
+ 1.5% cyflufenamid coating particle agent of 5 9.5% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 9.5%
Cyflufenamid 1.5%
Polyethylene glycol 3%
High dispersive silicic acid 1%
Calcium carbonate Complement to 100%
It in a mixer, will be on levigate active constituent even spread to the carrier soaked by polyethylene glycol.It can obtain by this method Obtain coating particle agent.
+ 1.5% cyflufenamid of 6 10.5% fluorine azoles bacterium acyl azanol of embodiment squeezes out granule
Fluorine azoles bacterium acyl azanol 10.5%
Cyflufenamid 1.5%
Sodium lignin sulfonate 4%
Attapulgite complements to 100%
Fluorine azoles bacterium acyl azanol 10.5%
Cyflufenamid 1.5%
Sodium lignin sulfonate 4%
Attapulgite Complement to 100%
Active component is mixed and ground with auxiliary agent, composition is soaked with water.The composition is squeezed out, then in the air stream It is dry.
+ 5% cyflufenamid suspension seed-coating agent of 7 30% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 30%
Cyflufenamid 5%
Polyoxyethylene tri-styryl phenol (10-20mol ethylene oxide) 2%
Xanthan gum 1%
Hydroxyethyl cellulose 1%
Glycerine 5%
PVP-K30 1%
Water Complement to 100%
Seed coat agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.
Oil-suspending agent can be dispersed in+10% cyflufenamid of 8 40% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 40%
Cyflufenamid 10%
Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Bentonite 1%
Glycerine 5%
PVP-K30 1%
Water 3%
Corn oil Complement to 100%
Dispersible oil-suspending agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.
+ 3% cyflufenamid microcapsule suspension-suspendinagent agent of 9 18% fluorine azoles bacterium acyl azanol of embodiment
Capsule core:
Cyflufenamid 3%
SOLVESSOTM200 4%
Toluene di-isocyanate(TDI) 1.5%
Polymethylene-polyphenyl isocyanates 3.5%
Emulsogen 3510 (from Clariant) 1.5%
Capsule wall:
1,6- hexamethylene diamine 1.3%
Water phase:
Fluorine azoles bacterium acyl azanol 18%
ATLOX ®4913 (from Croda) 2.7%
Synperonic PE/64 1.5%
Xanthan gum 0.25%
Defoaming agent 1%
Urea 1%
1,2- propylene glycol 1.2%
Methylisothiazolinone 2%
Catalyst 0.1%
Water Complement to 100%
By SOLVESSOTM200, toluene di-isocyanate(TDI), cyflufenamid, polymethylene-polyphenyl isocyanates, The oil that Emulsogen 3510 (from Clariant) is formed is added to the aqueous solution of hexamethylene diamine containing 1,6-, ATLOX 4913 In, form emulsion.Then it heats and keeps the temperature 50oIt is added catalyst reaction 2 hours under C.Cyflufenamid is obtained after cooling Microcapsules.
Synperonic PE/64 (from Croda), fluorine azoles bacterium acyl azanol, defoaming agent, urea, water mix in proportion It is even, and be sanded, it is prepared into fluorine-containing azoles bacterium acyl azanol water continuous phase.
In the water continuous phase for the fluorine-containing azoles bacterium acyl azanol that microcapsules containing cyflufenamid is added to, obtain of the invention micro- Capsule suspension-suspending agent.
+ 7% cyflufenamid suspending agent of 10 42% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 42%
Cyflufenamid 7%
Methyl naphthalene sulfonate formaldehyde condensate 5%
Dioctyl succinate disulfonate acid 5%
Xanthan gum 0.3%
Glycerine 5%
Defoaming agent 0.6%
Water Complement to 100%
The each components such as active component, dispersing agent, wetting agent and water are uniformly mixed according to the ratio of formula, it is ground and/or high Speed shearing controls partial size at 2 μm hereinafter, obtaining+7% cyflufenamid suspending agent of 42% fluorine azoles bacterium acyl azanol.
+ 2% cyflufenamid electrostatic oil agent of 11 10% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 10%
Cyflufenamid 2%
Ethoxylated castor oil 2%
Calcium dodecyl benzene sulfonate 3%
SOLVESSOTM100 Complement to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 5% cyflufenamid water dispersible granules of 12 28% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 28%
Cyflufenamid 5%
MODIFIED LIGNOSULPHONATE 2%
Lauryl sodium sulfate 2%
Ammonium sulfate 2%
Attapulgite Complement to 100%
Fluorine azoles bacterium acyl azanol, cyflufenamid active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed in the ratio of formula It closes uniformly, by air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.It is obtained after dry screening + 5% cyflufenamid water dispersible granules of 28% fluorine azoles bacterium acyl azanol.
+ 3.5% cyflufenamid aqueous emulsion of 13 11.5% fluorine azoles bacterium acyl azanol of embodiment
Oily phase:
Fluorine azoles bacterium acyl azanol 11.5%
Cyflufenamid 3.5%
N-Methyl pyrrolidone 20%
Polystyrene 3.5%
Water phase:
Xanthan gum 0.1%
Naphthalene sulfonic acids-formaldehyde condensation products sodium salt of sulfonation 2%
Fungicide 0.2%
Water Complement to 100%
Fluorine azoles bacterium acyl azanol and cyflufenamid are dissolved in N-Methyl pyrrolidone, polystyrene is added and obtains oily phase；According to Component in formula, which is uniformly mixed, obtains water phase；The oil phase is added to the aqueous phase under stiring obtains aqueous emulsion.
+ 30% cyflufenamid microemulsion of 14 5% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 5%
Cyflufenamid 30%
Ethoxylated castor oil 3%
Calcium dodecyl benzene sulfonate 2%
SOLVESSOTM100 20%
Water Complement to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 80% cyflufenamid of 15 20% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 20%
Cyflufenamid 80%
Fluorine azoles bacterium acyl azanol, cyflufenamid are proportionally uniformly mixed.
+ 90% cyflufenamid of 16 10% fluorine azoles bacterium acyl azanol of embodiment
Fluorine azoles bacterium acyl azanol 10%
Cyflufenamid 90%
Fluorine azoles bacterium acyl azanol, cyflufenamid are proportionally uniformly mixed.
Proportion is weight per distribution ratio in above embodiments.
Biological test example
One, virulence is tested:
The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture are calculated according to Sun Yunpei's Method, when CTC≤80, are then combined Object shows antagonism, and when 80<CTC<120, then composition shows summation action, and when CTC>=120, then composition shows Synergistic effect out.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture Percentage composition
Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100
Phytopathogen and host plant used in the test of greenhouse virulence
Test one: the toxicity test of the pathogen of Botrytis cinerea
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days the pathogen of Botrytis cinerea will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 1: to the virulence test result of Botrytis cinerea
As known from Table 1, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to grey grape The co-toxicity coefficient of spore is all larger than 120, shows as synergistic effect.
Test two: the toxicity test of wheat septoria
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days wheat septoria bacterium will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 2: to the virulence test result of wheat septoria
As known from Table 2, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to wheat shell Needle spore co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment three: the indoor toxicity test of fluorine azoles bacterium acyl azanol and cyflufenamid to Cercosporaarachidicola
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days Cercosporaarachidicola bacterium will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 3: to the virulence test result of Cercosporaarachidicola bacterium
As known from Table 3, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to peanut Tail spore bacterium co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment four: the indoor toxicity test of fluorine azoles bacterium acyl azanol and cyflufenamid to rod method apple specialized form bacterium
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days rod method apple specialized forms will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 4: to the virulence test result of rod method apple specialized form
As known from Table 4, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to rod method Apple specialized form co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment five: fluorine azoles bacterium acyl azanol and cyflufenamid are to the indoor toxicity test for justifying nuclear cavity bacteria
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days circle nuclear cavity bacterias will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by fungus block The toxic PDA culture medium center being configured in advance is moved to, culture, every processing in 25 DEG C of incubators is subsequently placed in and is repeated 4 times.3 days Afterwards, each processing colony diameter cm is measured using crossing method slide calliper rule, finds out correction and inhibits percentage.Each bacterium colony cross is handed over Fork surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 5: to the virulence test result of circle nuclear cavity bacteria
As known from Table 5, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to circle nuclear cavity Bacterium co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment six: the indoor toxicity test of fluorine azoles bacterium acyl azanol and cyflufenamid to the withered ball cavity bacteria of wheat grain husk
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days withered ball cavity bacterias of wheat grain husk will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 6: to the virulence test result of the withered ball cavity bacteria of wheat grain husk
As known from Table 6, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to wheat grain husk Withered ball cavity bacteria co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Experiment seven: the indoor toxicity test of fluorine azoles bacterium acyl azanol and cyflufenamid to pear cucumerinum
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days pear cucumerinums will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 7: to the virulence test result of pear cucumerinum
As known from Table 7, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to the black star of pears Germ co-toxicity coefficient is all larger than 120, shows as synergistic effect.
Test eight: to the toxicity test of standing grain white powder wheat specialized form bacterium
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days standing grain powdery mildew wheat specialized forms will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with connecing Fungus block is moved to the toxic PDA culture medium center being configured in advance by kind needle, is subsequently placed in culture in 25 DEG C of incubators, every processing weight It is 4 times multiple.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each Two diameters are surveyed in bacterium colony right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 8: to the virulence test result of standing grain powdery mildew wheat specialized form
As known from Table 8, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to standing grain white powder The co-toxicity coefficient of bacterium wheat specialized form is all larger than 120, shows as synergistic effect.
Test nine: the toxicity test of Puccinia recondita f. sp. tritici
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days Puccinia recondita f. sp. triticis will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4 It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 9: to the virulence test result of Puccinia recondita f. sp. tritici
As known from Table 9, it is hidden to wheat when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50 The co-toxicity coefficient of handle rest fungus hidden is all larger than 120, shows as synergistic effect.
Test ten: the toxicity test of grape snag shell bacterium
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days grape snag shell bacterium will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 10: to the virulence test result of grape snag shell bacterium
As known from Table 10, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to grape hook The co-toxicity coefficient of silk shell bacterium is all larger than 120, shows as synergistic effect.
Test 11: the toxicity test of melon monofilament shell powdery mildew
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days melon monofilament shell powdery mildews will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 11: to the virulence test result of melon monofilament shell powdery mildew
As known from Table 11, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to melon list The co-toxicity coefficient of silk shell powdery mildew is all larger than 120, shows as synergistic effect.
It tests 12: avenging the toxicity test of the withered bacterium of mould leaf
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days withered bacterium of the mould leaf of snow will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 12: to the virulence test result for avenging the mould withered bacterium of leaf
As known from Table 12, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to the mould leaf of snow The co-toxicity coefficient of withered bacterium is all larger than 120, shows as synergistic effect.
Test 13: the toxicity test of fusarium culmorum
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days fusarium culmorums will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 13: to the virulence test result of fusarium culmorum
As known from Table 13, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to yellow sickle The co-toxicity coefficient of knife bacterium is all larger than 120, shows as synergistic effect.
Test 14: the toxicity test of Rhizoctonia solani Kuhn
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days Rhizoctonia solani Kuhns will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 14: the virulence test result of Rhizoctonia solani
As known from Table 14, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, oppose withered silk The co-toxicity coefficient of pyrenomycetes is all larger than 120, shows as synergistic effect.
Test 15: the toxicity test of citrus Penicillium notatum
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days citrus Penicillium notatums will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 15: to the virulence test result of citrus Penicillium notatum
As known from Table 15, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to Citrus The co-toxicity coefficient of mould is all larger than 120, shows as synergistic effect.
Test 16: to the toxicity test of phytophthora infestans
Using inhibition mycelial growth rate method:
Fluorine azoles bacterium acyl azanol and cyflufenamid are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The fluorine azoles bacterium acyl azanol of different ratio and cyflufenamid series of concentrations compound solution be made with same method toxic Culture medium.2 days phytophthora infestans will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC in inhibiting is calculated with least square method50, then according to Sun Yunpei's Method calculating co-toxicity coefficient (CTC).
Table 16: to the virulence test result of phytophthora infestans
As known from Table 16, when the combination of fluorine azoles bacterium acyl azanol and cyflufenamid is in the range for matching 50:1-1:50, to pathogenic epidemic disease The co-toxicity coefficient of mould is all larger than 120, shows as synergistic effect.

Claims (19)

1. a kind of bactericidal composition, it is characterised in that: the bactericidal composition contains active constituent fluorine azoles bacterium acyl azanol and ring fluorine Bacterium amine, wherein the weight percent of fluorine azoles bacterium acyl azanol and cyflufenamid be 50:1-1:50, further preferably 40:1-1:40, It is further preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 10:1-1:10, more preferable 5:1-1:5.
2. bactericidal composition according to claim 1, it is characterised in that: the fluorine azoles bacterium acyl azanol and cyflufenamid quality The sum of account for the 1%-90%, preferably 5%-80%, more preferable 10%-80%, more preferable 15%-70% of the Bactericidal composite amount of substance.
3. bactericidal composition according to claim 1, it is characterised in that: the bactericidal composition, dosage form be aerosol, Capsule suspension, harl concentrating agents, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, instant solution, sprayable pulvis, can Emulsion concentrate, oil-in-water emulsion, water-in-oil emulsion, bulky grain agent, fine granule, the dispersible powder of oil, oil are miscible Flowable concentrating agents, oily miscible liquids, foaming agent, paste, suspension concentrating agents, solvable concentrating agents, suspending agent, seed coat agent, can Wet powder, water dispersible granules, soluble powder, microcapsule suspending agent, coated granule, squeeze out granule, missible oil, microemulsion, The cold atomization preparation of aqueous emulsion, effervescent tablet, ultra low volume liquids, suspoemulsion, ultra-low volume, super-low capacity calorimetric atomization preparation, double-contracting Fill (twin pack), seed treatment dry powder doses, seed treatment emulsion, seed treatment suspending agent, seed treatment liquor, at seed It manages dispersible pulvis, seed treatment microcapsule suspending agent, seed treatment gel, suspoemulsion, milk particle agent, ultra-low volume suspending agent, surpass Low capacity liquor, dispersibility concentrating agents.
4. bactericidal composition according to claim 1, it is characterised in that: also include filler and/or surfactant.
5. a kind of method that prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, it is characterised in that: will be described in claim 1 Bactericidal composition act on plant pathogen and/or its environment or plant, plant propagation material and the plant then grown In organ, soil or cultivation medium, material or space.
6. a kind of method that prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, it is characterised in that: will be described in claim 1 Fluorine azoles bacterium acyl azanol and cyflufenamid be administered simultaneously or apply respectively.
7. a kind of method that prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, it is characterised in that: infected it in plant It is preceding or after infecting by bactericidal composition described in claim 1 act on plant pathogen and/or its environment or plant, In plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.
8. a kind of method that prevention and treatment or prevention plant pathogenic fungi infect cultivated plant, it is characterised in that: will be described in claim 1 Bactericidal composition with agronomy is effective and the amount of application of basic plant-less toxicity is with seed treatment, foliage applying, stem application, leaching Thoroughly, the methods of instillation, casting, injection, spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment, or In plant, plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.
9. a kind of prevention and treatment or prevention plant pathogenic fungi infect the method for cultivated plant, it is characterised in that: including by claim 1 The bactericidal composition is applied to plant leaf surface.
10. a kind of prevention and treatment or prevention plant pathogenic fungi infect the method for cultivated plant, it is characterised in that: including by claim The plant organ that bactericidal composition described in 1 is applied to plant propagation material and then grows.
11. a kind of prevention and treatment or prevention plant pathogenic fungi infect the method for cultivated plant, it is characterised in that: including by claim Bactericidal composition described in 1 is applied to soil or cultivation medium.
12. bactericidal composition described in claim 1 is true on Cereal, veterinary antibiotics, ornamental plant and grapevine for preventing and treating The purposes of bacterium and bacterium.
13. bactericidal composition described in claim 1 is controlled on Cereal, veterinary antibiotics, ornamental plant and grapevine for controlling Botrytis (Botrytis) processed, Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), Pyricularia Sacc. (Pyricularia), false Cercosporalla (Pseudocercospora), Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), Puccinia (Puccinia), Phakopsora (Phakopsora), Ustilago (Llstilaginalcs), Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Uncinula (Uncinula), mycosphaerella (Mycosphaerella)), Phytophthora (Phytophthora), pythium (Pythium), Plasmopara (Plasmopara), peronospora (Peronospora), The purposes of Pseudoperonospora (Pseudoperonospora), disk stalk mould (Bremialactucae) harmful fungoid.
14. bactericidal composition described in claim 1 is for controlling grape grey mould, wheat leaf blight, peanut morning pinta, Ma Ling Potato early blight, the late blight of potato, wheat powdery mildew, net blotch of barley, wheat glume blotch, cucumber downy mildew, downy mildew of garpe, Soybean rust, wax gourd downy mildew, wax gourd anthracnose, tomato late blight, leaf muld of tomato, mandarin tree shot hole, mandarin tree anthrax Disease, scab of cucumber, cucumber blight dis-ease, pepper anthracnose, capsicum epidemic disease, black scurf of potato, fruit white rot of grape, grape downy mildew Disease, sponge gourd downy mildew, brown rust of wheat, early blight of tomato, uncinula necator, watermelon anthrax, sigatoka, cucumber white powder Disease, wheat avenge mould leaf blight, wheat scab, lawn snow mold, rice sheath blight disease, rice blast, rice green smut, strawberry Powdery mildew, leaf muld of tomato, cucumber target disease, cucumber gray leaf spot, potato target spot, ring rot of apple, apple tree early epidemic Disease, apple peels and cores, scab of apple, Spike-stalk Brown Spot of Grape, watermelon powdery mildew, watermelon grafting, banana foxiness Disease, banana freckle, sigatoka, take-all, jujube tree rust, the leaf blight of corn, corn southern leaf blight, watermelon anthrax Disease, watermelon grafting, rice green smut, target, tea tree anthracnose, graw mold of tomato, Brown patch disease, coffee rust Disease, wheat rust, sclerotinia sclerotiorum, scab of cucumber, pear scab, lichee anthracnose, bitter rot or anthracnose of grape, rice rice are bent Disease, maize head smut, head smut of sorghum, loose smut of wheat, barley banded sclerotial blight, Penicillium italicum, citrus common green mold, grass Certain kind of berries powdery mildew, wheat leaf rust, gray mold of cucumber.
15. bactericidal composition described in claim 1 is for protecting plant, plant propagation material and the plant organ then grown Purposes.
16. causing a disease in place prevention and treatment soil or cultivation medium that bactericidal composition described in claim 1 is prevented and treated needed for being applied to Or the purposes of saprophytic fungi and bacterium.
17. bactericidal composition described in claim 1 is for handling seed to protect seed to invade from the phytopathogen of carrying The purposes attacked.
18. the purposes that bactericidal composition described in claim 1 is used to protect stock.
19. bactericidal composition described in claim 1 is for protecting stock in storage period from fungi or the use of bacterial invasion On the way.
CN201710909455.9A 2017-09-29 2017-09-29 A kind of bactericidal composition Withdrawn CN109566626A (en)

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