CN109549734A - A kind of mouse periodontitis modeling method based on photocuring fluid resin - Google Patents
A kind of mouse periodontitis modeling method based on photocuring fluid resin Download PDFInfo
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- CN109549734A CN109549734A CN201811267858.9A CN201811267858A CN109549734A CN 109549734 A CN109549734 A CN 109549734A CN 201811267858 A CN201811267858 A CN 201811267858A CN 109549734 A CN109549734 A CN 109549734A
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- 229920005989 resin Polymers 0.000 title claims abstract description 31
- 239000011347 resin Substances 0.000 title claims abstract description 31
- 201000001245 periodontitis Diseases 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000012530 fluid Substances 0.000 title claims abstract description 20
- 238000000016 photochemical curing Methods 0.000 title claims description 17
- 238000011049 filling Methods 0.000 claims abstract description 12
- 238000005530 etching Methods 0.000 claims abstract description 11
- 239000000853 adhesive Substances 0.000 claims abstract description 9
- 230000001070 adhesive effect Effects 0.000 claims abstract description 9
- 239000000523 sample Substances 0.000 claims abstract description 4
- 241000699666 Mus <mouse, genus> Species 0.000 claims abstract 6
- 241000699670 Mus sp. Species 0.000 claims abstract 6
- 210000004195 gingiva Anatomy 0.000 claims abstract 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract 2
- 210000003254 palate Anatomy 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 8
- 210000000214 mouth Anatomy 0.000 claims description 8
- 238000001723 curing Methods 0.000 claims description 5
- 206010002091 Anaesthesia Diseases 0.000 claims description 4
- 230000037005 anaesthesia Effects 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 210000003128 head Anatomy 0.000 claims description 3
- 208000002599 Smear Layer Diseases 0.000 claims description 2
- 238000005115 demineralization Methods 0.000 claims description 2
- 230000002328 demineralizing effect Effects 0.000 claims description 2
- 210000004268 dentin Anatomy 0.000 claims description 2
- 238000007912 intraperitoneal administration Methods 0.000 claims description 2
- 210000004357 third molar Anatomy 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims 1
- 239000004840 adhesive resin Substances 0.000 claims 1
- 229920006223 adhesive resin Polymers 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 229960001412 pentobarbital Drugs 0.000 claims 1
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 claims 1
- 238000010172 mouse model Methods 0.000 abstract description 3
- 210000000683 abdominal cavity Anatomy 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 210000000988 bone and bone Anatomy 0.000 description 12
- 210000004261 periodontium Anatomy 0.000 description 8
- 208000010266 Aggressive Periodontitis Diseases 0.000 description 4
- 241000605862 Porphyromonas gingivalis Species 0.000 description 4
- 201000006727 periodontosis Diseases 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 210000001909 alveolar process Anatomy 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 238000010603 microCT Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 2
- 240000001624 Espostoa lanata Species 0.000 description 2
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- 238000011161 development Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 230000003239 periodontal effect Effects 0.000 description 2
- 239000003504 photosensitizing agent Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 206010004053 Bacterial toxaemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000025157 Oral disease Diseases 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
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- 238000010171 animal model Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 208000001277 chronic periodontitis Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
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- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000002050 maxilla Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61C—DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE
- A61C19/00—Dental auxiliary appliances
- A61C19/003—Apparatus for curing resins by radiation
Landscapes
- Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dentistry (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)
- Dental Preparations (AREA)
Abstract
本发明公开了一种基于光固化流体树脂的小鼠牙周炎建模方法，包括：待建模小鼠腹腔麻醉，固定小鼠头部，用开口器使小鼠稳定张口，暴露上颌左上或右上磨牙区，生理盐水清洁所述上磨牙区内磨牙腭面、龈沟和腭侧牙龈并充分干燥，用自酸蚀树脂粘接剂涂布磨牙腭面、龈沟和腭侧牙龈，光固化5‑10s，用光固化流体树脂沿磨牙龈沟充填，使光固化流体树脂覆盖腭面、龈沟和腭侧牙龈且不覆盖磨牙咬合面，光固化30s，形成局部充填物，用探针确定其稳定度即可，成模仅需一次操作，小鼠模型死亡率低且成功率高，对临床治疗牙周炎的研究具有重要推动作用。
The invention discloses a method for modeling periodontitis in mice based on light-cured fluid resin, comprising: anesthetizing the mouse to be modeled in the abdominal cavity, fixing the head of the mouse, using an opener to stably open the mouse's mouth, and exposing the upper left or right upper molar of the upper jaw. area, the molar palatal surface, gingival sulcus and palatal gingiva in the upper molar area were cleaned with saline and fully dried, and the molar palatal surface, gingival sulcus and palatal gingiva were coated with self-etching resin adhesive, and light-cured 5‑ For 10s, fill along the molar sulcus with light-cured fluid resin, so that the light-cured fluid resin covers the palatal surface, gingival sulcus and palatal gingiva and does not cover the occlusal surface of the molars, light-cured for 30s to form a local filling, and use a probe to confirm its stability Only one operation is needed to form a model, and the mouse model has a low mortality rate and a high success rate, which plays an important role in promoting the clinical treatment of periodontitis.
Description
Technical field
The invention belongs to Periodontics technical fields, and in particular to a kind of mouse periodontitis based on photocuring fluid resin
Modeling method.
Background technique
Periodontosis is the principal element for endangering one of common oral disease of human health, and adult is caused to lose tooth.
Periodontium includes gum, parodontium, alveolar bone, cementum, and the occurrence and development of periodontosis are mainly shown as the thin of periodontium
The chronic infection of bacterium property causes the progressive inflammatory destruction of periodontium, ultimately causes absence of tooth, greatly compromises oral cavity chewing
The function of system affects the whole body health of patient.Bacterial plaque microorganism is the initiating agent of chronic periodontitis, and main pathogenic bacteria is
Porphyromonas gingivalis, by epidemiological survey, domestic periodontosis illness rate is high, and patient groups' radix is big, and early stage periodontal
Scorching clinical manifestation is unobvious, and patient easily ignores the timely diagnosis and treatment to the disease.Representative of the mouse as laboratory rodent,
Fast with reproduction speed, the local tissue structures feature similar with the mankind, therefore, utilization are built by the experimental animal of representative of mouse
Vertical Experimental Periodontitis model inquires into the cause of disease of periodontitis for researcher and pathologic process provides advantageous study condition.
Traditional mouse Periodontitis Model method for building up includes locally injecting LPS method, locally injecting porphyromonas gingivalis
Bacterium solution method and silk thread desmurgia etc..Locally injecting LPS causes transient toxaemia when having the disadvantage that 1. injection, models small
The mouse death rate is high；2. the next day of needing, injects, with injection need to anaesthetize mouse systemic, continuous general anesthesia easily lead to mouse because
Intestinal obstruction and it is dead, modeling success rate it is low.Locally injecting porphyromonas gingivalis bacterium solution method for locally injecting LPS,
Although modeling period shortens, additional culture porphyromonas gingivalis is needed, is operated more many and diverse.The technology of silk thread desmurgia is sensitive
Property it is higher, and since mouse buccal vestibule ditch is shallower, when modeling, easily causes the big bleeding in part.
Summary of the invention
Technical problem underlying solved by the invention be evade current mouse periodontitis modeling method there are the shortcomings that, provide
A kind of mouse periodontitis modeling method based on photocuring fluid resin.
To achieve the above object, the present invention adopts the following technical scheme:
Mouse periodontitis modeling method based on photocuring fluid resin, comprising the following steps:
(1) mouse peritoneal to be modeled is anaesthetized with the anesthetic that mass concentration is 1.5% yellow Jackets, anaesthesia dosage is
40mg/kg；
(2) fixed mouse head makes mouse stabilization dehisce with opener, and exposure upper jaw upper left or upper right are ground one's teeth in sleep area, physiology
Salt water cleans the palatal surface ground one's teeth in sleep in area of grinding one's teeth in sleep on described, gingival sulcus and palate side gum, sufficiently dry with dry cotton ball；
(3) with from palatal surface, gingival sulcus and the palate side gum ground one's teeth in sleep described in the coating of acid etching resin adhesive, photocuring 5- is carried out
10s；
(4) it is filled, is made described in the photocuring fluid resin covering with the gingival sulcus that light curing liquid resin is ground one's teeth in sleep described in
Palatal surface, gingival sulcus and palate side gum and occlusal surface of grinding one's teeth in sleep is not covered, after photosensitizer is added, is in shortwave blue light, light source penetration capacity
Under the oral cavity light-solidified lamp of 4mm, for distance to photocuring 30s at photocuring plane 2mm, being formed has the nearly palatal surface high and close
Partial stowage's object on the low inclined-plane in the gum face；
(5) stability of partial stowage's object is determined with probe；If filling is firm to complete the modeling of mouse periodontitis,
If filling built on the sand or falling off, step (1) to step (4) is repeated until filling is firm.
Preferably, described to grind one's teeth in sleep including one or two or more in first molar, second molar or third molar.
Preferably, described to be made of from acid etching resin adhesive weak etching agent, silane coupling agent and adhering resin, being formed includes changing
The bonding mixed layer of property smear layer, dentine demineralization layer and priming coat.
Preferably, in step (3), coating is described to carry out acid etching to the palatal surface ground one's teeth in sleep from before acid etching resin adhesive.
The beneficial effects of the present invention are:
Mouse represents as laboratory rodent, the spy fast with reproduction speed, periodontium structure is similar with the mankind
Point, but also because it is dissected, small volume, inflammation resistance is weaker and oral cavity self-cleaning action is preferably not easy to establish Periodontitis Model, this hair
The unclean mouse periodontitis mould for causing periodontitis in part caused by bright initiative simulation building is overhang because of bonding agent residual with charges
Type has and simulates clinical periodontitis occurrence and development pathogeny and characteristics, and Cheng Mo only needs once-through operation, and the mouse model death rate is low and successfully
Rate is high, has important impetus to the research of clinical treatment periodontitis.
Detailed description of the invention
Fig. 1 is the mouse alveolar bone Overall View (crown root to) of control sides.
Fig. 2 is the mouse alveolar bone Overall View (crown root to) of experimental side.
Fig. 3 is the mouse palate side alveolar bone Overall View (cheek palate to) of control sides.
Fig. 4 is the mouse palate side alveolar bone Overall View (cheek palate to) of experimental side.
Fig. 5 is the mouse molar teeth sagittal section periodontium Overall View of control sides.
Fig. 6 is the mouse molar teeth sagittal section periodontium Overall View of experimental side.
Fig. 7 is mouse modeling schematic diagram (left side is control sides, and right side is experimental side).
Fig. 8 is to scheme (left side is control sides, and right side is experimental side) in mouse model mouth.
Specific embodiment
The technical solution that the invention will now be described in detail with reference to the accompanying drawings, but protection scope of the present invention is not limited to following realities
Apply example.
Embodiment
8 week old C57/BL6 mouse 12,24-26g are provided by Nanjing south model animal, are raised in the court's animal center
SPF grade environment in, this experiment obtain Ethics Committee, the court license.
Photocuring fluid resin is provided by Japanese illusion-colour company.
It is provided from acid etching resin adhesive by Japanese illusion-colour company.
Micro-CT is provided by Scanco Medical company, Switzerland, model Micro-CT50.
Mouse Periodontitis Model construction method based on photocuring fluid resin is as follows:
After a week, with 1.5% yellow Jackets intraperitoneal anesthesia mouse, dosage is 12 C57/BL6 mouse adaptable feds
40mg/kg, fixed mouse head make mouse stabilization dehisce with opener, and exposure upper jaw upper left is ground one's teeth in sleep area；It is cleaned with physiological saline
The upper jaw first, second, third is ground one's teeth in sleep palatal surface, gingival sulcus and palate side gum, sufficiently dry with the area dry cotton ball Shi Shu.With from acid etching resin
The bonding agent coating upper jaw first, second, third is ground one's teeth in sleep palatal surface, gingival sulcus and palate side gum, photocuring 5-10s, then mobility in using
Photocuring fluid resin along the upper jaw first, second, third grind one's teeth in sleep palate side gingival sulcus filling, make resin cover palate side facing, gingival sulcus and
Palate side gum, after photosensitizer is added, under shortwave blue light, the oral cavity light-solidified lamp that light source penetration capacity is 4mm, distance is to light
Solidify photocuring 30s at plane 2mm, partial stowage's object is made to form nearly palatal surface height, the low inclined-plane in nearly gum face, photocuring fluid tree
Rouge does not cover occlusal surface of grinding one's teeth in sleep.The stability of charges is determined using probe, completes operation, every two daily test after confirmation filling is firm
Look into whether charges in mouth consolidate, if falling off, re-operation.Wherein, upper jaw upper right is ground one's teeth in sleep as control sides, without any
Processing, as a result referring to Fig. 7 and 8.
Above-mentioned modeling method is shown in Table 1 compared with LPS/ bacterium solution injection method in the prior art and silk thread desmurgia.
Table 1
Effect example
The mouse Periodontitis Model of above-mentioned building is subjected to imaging Analysis, method are as follows: with 4% paraformaldehyde perfusion fixation
It impregnates 24 hours afterwards, dissects mouse maxilla and maxillary molar, micro-CT scanning and reconstruction analysis, layer are swept with a thickness of 10um.
Iconography micro-CT analyzes result as shown in figures 1 to 6, specifically:
1, palate side alveolar bone Overall View (cheek palate to) (Fig. 3 and 4), it is seen that maxillary molar modeling area's palate side alveolar bone is obviously inhaled
It receives, alveolar ridge blur margin, and deposits a large amount of Bone resoiption pits.
2, alveolar bone Overall View (crown root to) (Fig. 1 and 2), it is seen that it is obvious that maxillary molar models area's palate side absorption of alveolar bone,
Alveolar ridge blur margin, it is seen that Bone resoiption pit.
3, it grinds one's teeth in sleep sagittal section periodontium Overall View (Figures 5 and 6), it is seen that it is broadening that maxillary molar models area's periodontal spaces,
Alveolar ridge blur margin, absorption of alveolar bone is into root of the tooth lower 1/3.
In conclusion the present invention is based on photocuring fluid resins to establish mouse Periodontitis Model, by mouse modeling office
Organize row imaging Analysis in portion, it was demonstrated that this method can be successfully established mouse Periodontitis Model, sufficiently simulate the disease of clinical periodontosis
Electrophysiologic procedure.
The above is presently preferred embodiments of the present invention, but the present invention should not be limited to disclosed in the embodiment
Content.So all do not depart from the lower equivalent or modification completed of spirit disclosed in this invention, the model that the present invention protects is both fallen within
It encloses.
Claims (4)
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| CN201811267858.9A CN109549734A (en) | 2018-10-29 | 2018-10-29 | A kind of mouse periodontitis modeling method based on photocuring fluid resin |
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| CN201811267858.9A CN109549734A (en) | 2018-10-29 | 2018-10-29 | A kind of mouse periodontitis modeling method based on photocuring fluid resin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113855305A (en) * | 2021-10-09 | 2021-12-31 | 重庆医科大学附属口腔医院 | Improved rat periodontitis inflammation molding method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350444A (en) * | 1999-04-16 | 2002-05-22 | 登弗泰克斯有限公司 | Method and apparatus for treating dental caries |
| CN1359278A (en) * | 1999-06-04 | 2002-07-17 | 登弗泰克斯有限公司 | Method and apparatus for filling dental root canal |
-
2018
- 2018-10-29 CN CN201811267858.9A patent/CN109549734A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350444A (en) * | 1999-04-16 | 2002-05-22 | 登弗泰克斯有限公司 | Method and apparatus for treating dental caries |
| CN1359278A (en) * | 1999-06-04 | 2002-07-17 | 登弗泰克斯有限公司 | Method and apparatus for filling dental root canal |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113855305A (en) * | 2021-10-09 | 2021-12-31 | 重庆医科大学附属口腔医院 | Improved rat periodontitis inflammation molding method |
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Application publication date: 20190402 |