CN1095377C - Medicine for treating skin ringworm and dermatitis and preparation process - Google Patents

Abstract

A medicine for treating skin ringworm and inflammation and a preparation method thereof, which is a medicament made of the following raw materials in proportion by weight: 25-35 grams of dried blood, 80-120 grams of curcuma, 100-200 grams of rhizoma coptis, 80-120 grams of alum, 100-200 grams of betel nut, 80-120 grams of aloe, 40-60 grams of Fructus Dafeng, 100-150 grams of Kochia scoparia, 40-60 grams of Mylabris, the present invention adopts the preparation of Chinese medicine Coptidis Rhizoma as the main agent, The dosage form is tincture or vinegar, which does not contain antibiotics, has low toxicity, small side effects, high curative effect and long validity period. It not only has obvious antibacterial effect on dermatophyte, but also has better bactericidal effect.

Description

Medicine for treating skin ringworm and inflammation and preparation method thereof

The invention relates to a medicine for treating skin, especially various skin inflammations and ringworm, and also relates to a preparation method of the medicine.

Skin ringworm is a common and frequently-occurring disease in skin diseases. It not only has severe pain and itching, which makes people unbearable, but also secondary infection sometimes leads to systemic symptoms. Though the medicine of present treatment is a lot, clean once as athlete's foot, often need long-term repeated medication, and many are irritating, corrosivity is big, and phenomenons such as skin comes off, rotten foot occur, and what have is only effective to exterminate tinea pedis. Bacteria are ineffective.

The purpose of the present invention is to provide a medicine for treating skin ringworm and inflammation with high curative effect, little irritation, good effect and convenient use and a preparation method thereof.

Another object of the present invention is to provide a preparation method of the medicine.

The object of the present invention is achieved like this: a kind of medicine for the treatment of dermatophytosis, inflammation and preparation method thereof, is characterized in that: it is the medicament that is made by the raw material of following weight ratio:

Dried blood 25-35 grams Turmeric 80-120 grams

Coptis 100-200 grams Alum 80-120 grams

Betel nut 100-200 grams Aloe vera 80-120 grams

Dafengzi 40-60 grams Kochia 100-150 grams

Spotted beetle 40-60 grams

Its optimal weight ratio:

Dried Blood 30g Turmeric 100g Coptidis Rhizome 150g

Alum 100g Betel nut 150g Aloe vera 100g

50 grams of Dafengzi 150 grams of Kochia chinensis 50 grams

The above-mentioned medicament is tincture or vinegar. Its tincture adopts 10000 milliliters, and concentration is that 70% alcohol soaks above-mentioned medicine, normal temperature and normal pressure 7-10 days, gets final product in filtration. The preparation method of vinegar adopts conventional method to prepare.

The present invention adopts the preparation of Coptis chinensis as the main medicine, and the dosage form is tincture or vinegar, which does not contain antibiotics, has low toxicity, small side effects, high curative effect and long validity period. It not only has obvious antibacterial effect on dermatophytes, but also has relatively Good bactericidal effect.

Describe the present invention in detail below in conjunction with implementation:

Embodiment one: take raw material by following proportioning:

Dried Blood 25g Turmeric 80g Coptidis Rhizome 100g

Alum 80g Betel nut 100g Aloe vera 80g

40 grams of Dafengzi 100 grams of Kochia chinensis 40 grams

Embodiment two: take raw material by following proportioning

Dried Blood 35g Turmeric 120g Coptis Rhizoma 200g

Alum 120g Betel nut 200g Aloe vera 120g

60 grams of Dafengzi 150 grams of Kochia chinensis 60 grams

Embodiment three: take by weighing raw material according to following optimal proportioning

Dried Blood 30g Turmeric 100g Coptidis Rhizome 150g

Alum 100g Betel nut 150g Aloe vera 100g

50 grams of Dafengzi 150 grams of Kochia chinensis 50 grams

The preparation of the above-mentioned raw materials all adopts the Pharmacopoeia of the People's Republic of China (95 editions).

The preparation method of the above-mentioned tincture is as follows: 10,000 milliliters of alcohol with a concentration of 75% is used to soak the above-mentioned component medicaments, soak for 7-10 days at normal temperature and under normal temperature, filter and bottle for use.

Anti-dermatophyte experiment of the present invention

Medium: Sabouraud's Dextrose Agar) Strains: Candida albicans, Trichophyton rubrum, T.interdigitale, Epidermophylon floccosu

method:

1. Prepare Sabouraud medium, fill it into a Erlenmeyer flask, and sterilize it with high pressure.

2. Get the dermatophyte and inject it into a sterilized petri dish (the diameter of the petri dish is 10cm) with an aseptic method, then pour into Sabouraud's base (every dish 20ml).

3. Each dish was inoculated with the above four kinds of molds (a 0.5 mm diameter cluster).

4. Moisturize and cultivate at 37°C, and observe the results after one week.

5. Observation method: measure the colony diameter (mm) and compare it with the control, observe the growth state of the colony and whether there is growth of miscellaneous bacteria.

As can be seen from Table 1, the present invention has obvious antibacterial action to dermatophytes (Trichomyces rubrum, Trichophyton gypsum and Epidermophyton flocculus) and deep fungus Candida albicans the same as clotrimazole ringworm medicine , making the colony shrink (n=5, P<0.001). Also get from table 1 and find out, antifungal effect of the present invention is greater than clotrimazole tinea potion, mainly shows in two aspects: one: when clotrimazole tinea potion is diluted 60 times, its antibacterial effect promptly disappears, and bacterial colony The diameter is close to that of the control group (n=5, P>0.05), while the present invention still has obvious antibacterial effect when diluted 120 times (n=5, P<0.001). Its two: clotrimazole ringworm liquid medicine and 10 times of dilution thereof can only make bacterium colony dwindle, and the present invention and 60 times of dilution thereof then make the bacterium colony of dermatophyte (red, stone, floc) disappear in the culture medium, 120 times The diluent still caused the colonies of Epidermophyton flocculus to disappear. It is suggested that the clotrimazole tinea potion can only inhibit the growth of fungi, but the present invention may also have a bactericidal effect. Can also find out from the table, the present invention has substantially disappeared (n=5, P＞0.05) to the effect of Candida albicans when 60 times of dilutions, and 120 times of dilutions are to dermatophyte (red, stone, floc) ) still had obvious antibacterial effect (n=5, P<0.001). It is suggested that the antibacterial effect of the present invention on epidermal fungi is greater than that on deep fungi.

Table 2: The present invention treats the curative effect analysis of dermatophytosis case disease name Number of cases cure (person) Significantly effective (person) Get better (person) invalid (person) Efficient(%) Relapse (person) Recurrence rate(%) athlete's foot 268 180 60 28 0 100 67 25 ringworm 54 26 20 8 0 100 19 35 Jock itch twenty one 16 3 5 0 100 5 23.8 Tinea capitis 9 4 2 2 1 1 11 total 352 226 85 40 1 99.7 92 26.1

Observation of clinical efficacy:

From May 1992 to February 1998, in the Dermatology Department of Hubei Three Gorges University School of Medicine, the Dermatology Department of Yichang Central Hospital, and the outpatient department of Yichang Cadre Rest Hospital Hospital, a total of 352 cases of skin ringworm were treated, including 182 cases of males and 182 cases of females. 170 cases.

Usage: Clean the skin, apply this medicine on the affected surface after the skin is dry, 2-3 times a day.

Judgment criteria for curative effect: ①Clinical cure: the itching, desquamation and peeling of skin ringworm disappeared, and the skin color returned to normal; The chapped symptoms disappear, desquamation and desquamation are relieved, and pruritus is still present; ④ invalid: no obvious improvement after medication;

Coptis chinensis is the rhizome, roots and leaves of Copitis chinesis Franch, a perennial herb of Ranunculaceae. Chinese medicine believes that Coptidis is bitter and cold in nature, and has the effects of clearing away heat and dampness, purging fire and detoxifying. It is mainly used to treat carbuncle, furuncle, fire poison, eczema, itching, red eyes, swelling and pain, etc. It can be taken orally or externally. Modern medicine believes that Coptidis Rhizome has a broad-spectrum antibacterial effect, and it is effective against various G+, G - Bacteria have a strong antibacterial effect, and it also has a wide and significant inhibitory effect on more than ten common pathogenic fungi. The active ingredient in Coptis chinensis is also antipyretic, analgesic and local anesthetic.

Analysis of the antibacterial effect of the present invention and treatment of skin infectious diseases:

Experimental Study

Material: Media Broth Agar

Strains Staphylococcus aureus, Bacillus typhi, Escherichia coli, and Pseudomonas aeruginosa are all strains preserved in the Department of Microbiology, School of Medicine, Hubei Three Gorges University.

Drug Pikang Tincture is composed of more than a dozen traditional Chinese medicines such as Coptis chinensis, all of which are provided by the Chinese Pharmacy of Yichang Central Hospital; Gentamicin Sulfate Injection is produced by Hubei Yichang Pharmaceutical Factory.

Animals Rabbits were provided by the Experimental Animal Center of Tongji Medical University, Wuhan.

Method and Results:

Anti-bacterial test Take the cultured broth for 6 hours (the number of bacteria is 900 million/mL), and dilute it to 1/500 with sterile saline. Take 50 sterile plates with a diameter of 9 cm, add 1 mL of the four diluted bacterial solutions, and then pour 30 mL of agar medium that has been cooled to 45 °C, and immediately rotate and mix. After solidification, use a sterilized glass tube with an outer diameter of 6mm to punch 5 holes on the plate, and take out the agar in the holes. Add 75% alcohol, gentamicin sulfate (gentamicin) injection, 100% medicament of the present invention (stock solution), 1.67% medicament of the present invention (1:60 dilution) and 0.83% medicament of the present invention (1:60 dilution) in 5 holes respectively. : 120 dilution) each 100uL. Cultivate in a 37°C incubator for 24 hours, and measure the inhibition zone.

As seen from Table 3, 100%, 1.67% and 0.83% of the medicament of the present invention have obvious bacteriostasis to Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, and this effect is all greater than 75% alcohol, suggesting the present invention The traditional Chinese medicine ingredients and 75% alcohol have antibacterial synergistic effect. It can also be seen from the table that the antibacterial effect of its stoste on Pseudomonas aeruginosa and Staphylococcus aureus is greater than that of gentamicin sulfate injection, which may be the theoretical basis for the good curative effect of the present invention on burns and wound infection after scalding.

Skin irritation test 25 rabbits, weighing 1.5-2kg, both male and female. They were randomly divided into five groups; group I rubbed both ears of rabbits with 75% alcohol; group II rubbed both ears of rabbits with gentamicin sulfate injection. Group III rubbed both ears of rabbits with the stock solution of the present invention; group IV rubbed both ears of rabbits with 1.67% of the present invention; group V rubbed both ears of rabbits with 0.83% of the present invention. Observe and record whether there are skin irritations such as erythema and edema on the skin of both ears.

The test results found that, except that the gentamicin sulfate injection did not have any skin irritation symptoms, the stock solution of the present invention, 1.67% and 0.83% concentrations could cause transient erythema in rabbit ears as well as 75% alcohol, but without edema and blisters , disappeared after an hour, which may be caused by the vasodilator effect of alcohol. It is suggested that Pikang tincture has no obvious skin irritation.

2. Observation of clinical efficacy

During the five-year period from May 1992 to February 1998, a total of 2,648 cases of various skin infections were treated in the outpatient departments of the Affiliated Hospital of Hubei Three Gorges University School of Medicine, Yichang Central Hospital, and Yichang Cadre Rest Hospital, including 865 male cases. 1783 cases were female. Method of use: clean the skin, apply this medicine on the lesion surface after the skin is dry, 2-3 times a day. Precautions: It is forbidden to make this drug into a drug gauze and apply it to the wound or stuff it deep into the wound. Judgment criteria for curative effect: ①Clinical cure: skin inflammation subsides, and wound surface heals; ②Marked effect: skin inflammation is significantly reduced, and wound surface shrinks by more than 80%; ③Improved: skin inflammation is alleviated, and wound surface shrinks by more than 60%; No significant improvement in inflammation; ⑤Recurrence: Acne patients relapsed within 1 month of continuous medication.

As can be seen from Table 4, among the 2648 patients, 216 were cured, 312 were markedly effective, and the total effective rate was 99.8%. 87 acne patients relapsed within one month of continuous medication, the recurrence rate was 71%, and the total recurrence rate was only 3.3%. Continuous use is still effective.

For general skin inflammation, it can be effective within 24 hours after taking the medicine, and it can be cured after three days. For skin ulcers that have not healed for a long time, it shows a good astringent effect after medication, which makes the wound surface dry and the ulcer surface shrinks. Generally, it can be cured after two weeks of continuous medication. It also has a good therapeutic effect on wound infection (including Pseudomonas aeruginosa infection) after burns and scalds, and most patients heal after continuous medication for 1-2 weeks.

In summary, the present invention is a traditional Chinese medicine preparation made of Chinese medicine Coptidis Rhizoma as the main medicine and formulated in a compatible formula, which does not contain antibiotics, has low toxicity, small side effects and high curative effect. During the trial process of more than 5 years, we found that the drug has the following advantages: ① High curative effect: For hands, tinea pedis and skin ringworm, it can produce antipruritic effect after 10 minutes of medication, and it will be effective after one week of continuous medication, and after one month Most of the symptoms of skin lesions disappeared, and most patients recovered after three months of continuous medication, and the recurrence rate was low (26.1%). Recurrence, for general skin inflammation, can be markedly effective within 24 hours after medication, and can be cured after three days. For skin ulcers that have not healed for a long time, it shows a good astringent effect after medication, which makes the wound surface dry and the ulcer surface shrinks. Generally, it can be cured after two weeks of continuous medication. It also has a good therapeutic effect on wound infection (including Pseudomonas aeruginosa infection) after burns and scalds, and most patients heal after continuous medication for 1-2 weeks. ②Slightly irritating to the skin, because the medicine is a tincture containing alcohol, it will not cause redness and swelling of the skin, and large flakes of skin except for short-term pain stimulation on the wound; ③This product does not contain corticosteroids and will not cause Pigmentation (this medicine is a traditional Chinese medicine preparation, it will leave the light yellow color of the traditional Chinese medicine itself on the skin after rubbing, and it will disappear after washing with soapy water); ④ This product does not contain antibiotics, and long-term use of this product will not produce drug resistance and Allergic reaction; ⑤ The drug has strong skin penetration and is easy to use, just rub it gently on the wound surface, and the drug can penetrate into the tissue to play a role; ⑥ The validity period of this product is more than 5 years.

Table 1: the antibacterial effect of the present invention (dermatosis net) on skin fungus

( x±S)

Colony (mm) Grouping Drug Concentration n

Rosary albicans Trichomonad rubrum Trichophyton gypsum Epidermophyma flocculus1 Blank - 12 8.3±3 36±9 22.9±9 21.0±62 Clotrimazole stock solution 5 0.5±0.2 ^*** 0.6±0.3 ^*** 0.8±0.2 ^{* **} 0.5±0.2 ^*** 3 Clotrimazole 1∶10 dilution 5 1.8±0.4 ^*** 0.8±0.4 ^*** 0.6±0.3 ^*** 1.0±0.1 ^*** 4 Clotrimazole 1∶60 dilution 5 6.5±2 42.0±3 ^24.0 ±4 23.2±25 Pishaojing stock solution 5 0.8±0.3 *** 0 ^*** 0 ^*** 0 ^*** 6 Pishaojing 1∶60 dilution 5 5.8±0.8 0 ^{** *} 0 ^*** 0 ^*** 7 Dishinajing 1∶120 5 5.8±0.8 0.18±0.08 ^*** 0.18±0.08 ^*** 0 ^*** 8 Dishinajing 1∶240 5 6.8±1 41.0±2 24.8±3 21.2±2

Compared with blank control: ***P<0.001

Table 3: Antibacterial effect of the present invention

Zone of Inhibition (mm) x±SD drug concentration n

(%) Staphylococcus aureus Typhi Escherichia coli Pseudomonas aeruginosa Alcohol 75 10 7.6±2 7.3±0.8 5.9±0.6 2.0±0.1 Gentamicin 6.78 10 15.2±2 ^*** 10.5±0.9 ^** 23.0±2 ^*** 12.7±2 ^*** Invention 100 10 23.0±2 ^*** 18.5±0.9 ^*** 22.7±1.5 ^*** 18.2±0.6 ^*** Invention 1.67 10 15.6±0.7 ^*** 12.6±0.9 ^** 12.0 ±1 ^*** 12.5±0.7 ^*** Invention 0.83 10 12.6±1 ^*** 10.3±0.8 9.7±0.9 ^*** 8.2±0.6 ^***

Table 4: Analysis of the curative effect of the present invention on treating skin infectious diseases

(Person) (person) (person) (person) (person) (person) (person) (person) (percent) ( %) ( %) youth acne 1230 986 174 70 0 100 87 7.1 sore 910 758 83 69 0 100 0 0 surgery and trauma infection and trauma infection 230 220 8 2 0 100 0 0 burns Burning infection 192 148 27 17 0 100 0 0 Do not heal skin ulcer 86 56 20 6 4 95 0 0 combination 2648 312 164 49.8 87 3.3

Claims (4)

1, a kind of medicine for the treatment of dermatophytosis, inflammation is characterized in that: it is the medicament that is made by the raw material of following weight ratio: Dried blood 25-35 grams Turmeric 80-120 grams Coptidis 100-200 grams Alum 80-120 grams Betel nut 100-200 grams Aloe vera 80-120 grams Dafengzi 40-60 grams Kochia 100-150 grams Cantharidin 40-60 grams. 2. A medicine for treating skin ringworm and inflammation according to claim 1, characterized in that: it is a medicament made of the following raw materials in weight ratio: Dried blood 30g turmeric 100g Coptis chinensis 150 grams Alumen 100 grams Betel nut 150g Aloe vera 100g Dafengzi 50g Kochia 150g Cantharidin 50 grams. 3. A medicine for treating skin ringworm and inflammation according to claim 1, characterized in that said medicine is tincture or vinegar. 4. The method for preparing a kind of medicine for treating skin ringworm and inflammation according to claim 1 or 3 is characterized in that: its tincture adopts 10000 milliliters, and the concentration is 70% alcohol to soak the above-mentioned medicament, and the 7- 10 days, just filter.