CN109536296B - Antibacterial biological detergent for infants and preparation method thereof - Google Patents
Antibacterial biological detergent for infants and preparation method thereof Download PDFInfo
- Publication number
- CN109536296B CN109536296B CN201811641083.7A CN201811641083A CN109536296B CN 109536296 B CN109536296 B CN 109536296B CN 201811641083 A CN201811641083 A CN 201811641083A CN 109536296 B CN109536296 B CN 109536296B
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- parts
- water
- soapberry
- fermentation liquor
- stirring
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- 239000003599 detergent Substances 0.000 title claims abstract description 66
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 94
- 238000000855 fermentation Methods 0.000 claims abstract description 72
- 230000004151 fermentation Effects 0.000 claims abstract description 72
- 240000003377 Shepherdia canadensis Species 0.000 claims abstract description 60
- 235000018324 Shepherdia canadensis Nutrition 0.000 claims abstract description 60
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 53
- 229930182490 saponin Natural products 0.000 claims abstract description 53
- 150000007949 saponins Chemical class 0.000 claims abstract description 53
- 229920001661 Chitosan Polymers 0.000 claims abstract description 45
- 238000003809 water extraction Methods 0.000 claims abstract description 39
- 239000006041 probiotic Substances 0.000 claims abstract description 36
- 235000018291 probiotics Nutrition 0.000 claims abstract description 36
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229930186217 Glycolipid Natural products 0.000 claims abstract description 34
- 239000003876 biosurfactant Substances 0.000 claims abstract description 28
- 239000003094 microcapsule Substances 0.000 claims abstract description 26
- 229920000805 Polyaspartic acid Polymers 0.000 claims abstract description 21
- 108010064470 polyaspartate Proteins 0.000 claims abstract description 21
- 239000008367 deionised water Substances 0.000 claims abstract description 19
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 19
- 108091005804 Peptidases Proteins 0.000 claims abstract description 18
- 239000004365 Protease Substances 0.000 claims abstract description 18
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 18
- 229940080421 coco glucoside Drugs 0.000 claims abstract description 14
- 239000004475 Arginine Substances 0.000 claims abstract description 13
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims description 53
- 241001122767 Theaceae Species 0.000 claims description 46
- 239000002245 particle Substances 0.000 claims description 32
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 24
- 239000000284 extract Substances 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 239000000661 sodium alginate Substances 0.000 claims description 18
- 229940005550 sodium alginate Drugs 0.000 claims description 18
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 17
- 235000010413 sodium alginate Nutrition 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 14
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 12
- 241000186660 Lactobacillus Species 0.000 claims description 12
- 235000018597 common camellia Nutrition 0.000 claims description 12
- 239000000839 emulsion Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 12
- 229940039696 lactobacillus Drugs 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 238000007873 sieving Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 241000186000 Bifidobacterium Species 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 108091005658 Basic proteases Proteins 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 7
- 230000001804 emulsifying effect Effects 0.000 claims description 7
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims description 7
- 244000199866 Lactobacillus casei Species 0.000 claims description 6
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 6
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 6
- 229940037003 alum Drugs 0.000 claims description 6
- 239000006286 aqueous extract Substances 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229940017800 lactobacillus casei Drugs 0.000 claims description 6
- 229940057995 liquid paraffin Drugs 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 5
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 5
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- -1 mannose erythritol lipid Chemical class 0.000 claims description 3
- 241000209507 Camellia Species 0.000 claims 2
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims 1
- 239000004386 Erythritol Substances 0.000 claims 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims 1
- 230000000845 anti-microbial effect Effects 0.000 claims 1
- 229940009714 erythritol Drugs 0.000 claims 1
- 235000019414 erythritol Nutrition 0.000 claims 1
- 238000000967 suction filtration Methods 0.000 claims 1
- 239000006260 foam Substances 0.000 abstract description 24
- 241000894006 Bacteria Species 0.000 abstract description 10
- 230000036541 health Effects 0.000 abstract description 7
- 239000008233 hard water Substances 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 230000002147 killing effect Effects 0.000 abstract description 2
- 231100000956 nontoxicity Toxicity 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract description 2
- 244000269722 Thea sinensis Species 0.000 abstract 1
- 231100000053 low toxicity Toxicity 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 48
- 230000000694 effects Effects 0.000 description 18
- 239000004744 fabric Substances 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 13
- 230000032798 delamination Effects 0.000 description 13
- 230000003385 bacteriostatic effect Effects 0.000 description 12
- 240000001548 Camellia japonica Species 0.000 description 10
- 239000004094 surface-active agent Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- 230000000529 probiotic effect Effects 0.000 description 8
- 238000005202 decontamination Methods 0.000 description 5
- 230000003588 decontaminative effect Effects 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 201000004624 Dermatitis Diseases 0.000 description 4
- 238000005562 fading Methods 0.000 description 4
- 238000005187 foaming Methods 0.000 description 4
- 230000007794 irritation Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 210000002374 sebum Anatomy 0.000 description 3
- 238000013112 stability test Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 239000006229 carbon black Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000011056 performance test Methods 0.000 description 2
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- 125000000647 trehalose group Chemical group 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000010495 camellia oil Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
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- 238000005238 degreasing Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
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- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
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- 230000000622 irritating effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/825—Mixtures of compounds all of which are non-ionic
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin
- C11D3/227—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin with nitrogen-containing groups
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/33—Amino carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/37—Polymers
- C11D3/3703—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C11D3/3719—Polyamides or polyimides
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/381—Microorganisms
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/662—Carbohydrates or derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Emergency Medicine (AREA)
- Microbiology (AREA)
- Detergent Compositions (AREA)
Abstract
The invention discloses an infant antibacterial biological detergent which is prepared from the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water. The infant antibacterial biological detergent disclosed by the invention is simple to prepare, has no toxicity or extremely low toxicity on the used components, has no stimulation to skin, has low residue, does not threaten the health of infants, is rich and fine in foam, strong in detergency, strong in hard water resistance and good in solubility, and has good killing and inhibiting effects on various bacteria and moulds.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to an infant antibacterial biological detergent and a preparation method thereof.
Background
Detergents and disinfectants are indispensable commodities in human social life and bear important duties of keeping environment clean and sanitary and ensuring human health and safety. With the gradual increase of the health consciousness of people, new requirements on detergents and disinfectants are also put forward. At present, the detergent and the disinfectant mainly have the problems that the dual requirements of washing and disinfection cannot be met simultaneously, the sterilizing effect of synthetic and plant-source bactericides is poor, unreasonable use causes harm to human health and the like.
The liquid detergent has the advantages of good solubility, no dust generation, less residue, convenient use and the like, and the demand in production, life and use is continuously increased. In order to meet the performance requirements of detergency, solubility, easy rinsing and the like, the existing liquid detergent is added with too many synthetic compounds, and some of the compounds are difficult to degrade and some of the compounds have toxic effects and are harmful to the environment and human health. The use of large amounts of synthetic surfactants in existing detergents has led to the annual expansion of contaminated waters. Although the surfactants with poor biodegradability are basically eliminated at present, the widely used surfactants are biodegradable. However, even if it isSome surfactants with low concentration can also generate a large amount of foam, and the large amount of foam which is not easy to disappear forms an isolation layer on the water surface, so that the gas exchange between the water body and the atmosphere is weakened, the water body smells, the aesthetic feeling of the natural water body landscape is influenced, and the biotoxicity of the surfactant also directly threatens the survival of aquatic animals and plants. The waste water containing the detergent is discharged into the water environment in large quantity, and the components thereof can be biodegraded to cause the exhaustion of dissolved oxygen and mineral substances in the water, so that the dissolved oxygen in the water is obviously reduced, and the concentration exceeds 8 mg.L-1It also can poison aquatic organisms and fish.
Most of stains, sweat, milk, oil and the like of baby products are protein, and most of the existing detergents adopt a mode of directly adding protease, and the protease is easy to inactivate or react with other detergents due to environmental pollution in the processes of storage, transportation and use, so that the stain removal effect is difficult to exert.
The detergent added with live probiotics and biological enzyme develops rapidly in recent years and obtains better effect, but the safety problem of the live probiotics also arouses attention of people, the risk of increasing pathogenic bacteria exists in the mutation and transfer of thalli, and the risk is higher especially for patients with low immunity. In addition, the proper conditions required by the activity of the live bacteria and the biological enzyme greatly limit the production, transportation and use range, and the cost is correspondingly improved.
Most of infant laundry detergents in the market at present mainly emphasize the decontamination function of detergent and neglects mildness and nontoxicity, and in addition, because the mouth is a tool for infants to explore the world, such as a piece of saliva towel, clothes, toys and the like, the infants are licked by mouth, and the residual irritative and toxic components on the infant laundry detergents after being washed pose threats to the health of the infants.
Disclosure of Invention
Aiming at the defects of the existing detergent, the invention provides the infant antibacterial biological detergent which has the advantages of sterilization and odor removal, good decontamination effect, easy biodegradation, no toxic or side effect and low cost.
The purpose of the invention is realized by the following technical scheme:
an antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water.
Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.
Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.
Further, the glycolipid biosurfactant is one or more of rhamnolipid, sophorolipid, trehalose glycolipid, cellobiolipid and mannosylerythritol lipid.
Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.
Further, theThe preparation method of the microcapsule for encapsulating protease comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.
A preparation method of an infant antibacterial biological detergent comprises the following steps:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.
The tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor used in the invention do not relate to an organic solvent in the whole preparation process, the fermentation liquor contains natural nonionic surfactant tea saponin and soapberry saponin, the toxicity and the irritation to the skin are very low, the surface tension of water can be reduced, the foam is rich and fine, the detergency is strong, the hard water resistance is strong, the dissolubility is good, and the degreasing property is moderate. Can also remove metal poisons on the surface of the human body, and has a high elution rate of lead, mercury, manganese, arsenic, chromium and other heavy metals up to 90 percent.
The glycolipid biosurfactant used in the invention can obviously reduce surface tension and has multiple functions of decontamination, emulsification, washing, dispersion, wetting, permeation, diffusion, foaming, antioxidation, viscosity regulation, sterilization, antistatic property and the like. The glycolipid biosurfactant has good thermal and chemical stability, and in addition, the trehalose glycolipid is resistant to strong acid and strong alkali, and the rhamnolipid has strong anti-mildew activity. The coco glucoside has excellent detergency, foaming, foam stabilizing, emulsifying, dispersing, solubilizing, wetting and penetrating abilities, acid resistance, alkali resistance and insensitivity to electrolyte. Can reduce irritation of other surfactants, and has good compatibility with skin and lower irritation.
The nano degradable chitosan used in the invention has the particle size of 100-500 nm, has good solubility in water, can rapidly enter the interior of the fabric, and is combined with the fabric through the protonated amino group to form a self-assembled film on the surface of the fabric, so that the fabric can be prevented from hardening, the skin-friendly property of the fabric is improved, and the nano degradable chitosan can play a role in moisturizing and skin care when contacting with the skin. The polyaspartic acid used in the invention can play a role in softening water, can cooperate with chitosan to soften fabrics, has certain moisturizing, sterilizing and softening effects on skin, has low irritation and toxicity, and has good biocompatibility with eyes and skin.
The encapsulated protease microcapsule is prepared by adopting chitosan-sodium alginate as a wall material and alkaline protease as a core material through a one-step emulsification method, has a controllable structure and good balling property, has an embedding rate as high as 65 percent, achieves a good encapsulating effect and a release function, and solves the problem that the activity of the alkaline protease is reduced due to interference of other washing components and influence of a washing environment in the storage and application processes of a detergent.
The tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor used in the invention greatly improve the purity of excellent natural nonionic surfactants tea saponin and soapberry saponin in the fermentation liquor through fermentation, the two surfactants have strong decontamination capability, stable property, good foamability and long foam fading time, have good removal effect on hydrophobic organic compounds, sebum, oil stain and the like, have low cost, safety and rapid fermentation and decomposition, do not cause any pollution to the environment, and do not bring negative problems of fluorescent agent, surfactant, environmental hormones, river eutrophication and the like. In addition, the tea saponin water extraction fermentation liquor contains tea saponin, various active ingredients with strong inhibition effect on escherichia coli, staphylococcus aureus and aspergillus niger, and the soapberry water extraction fermentation liquor contains a large amount of rhamnolipid besides soapberry saponin, and the rhamnolipid and the soapberry saponin are cooperated to enhance the decontamination effect.
The inactivated probiotics is added into the infant detergent, and in the washing process, the probiotic cells can be combined with the fabric and the chitosan self-assembly layer on the surface of the fabric and can be adhered to the surface of the skin when contacting the skin, so that other harmful bacteria, heavy metals and the like are prevented from being combined with the skin, and the incidence rate of infant eczema and dermatitis is reduced.
Compared with the prior art, the invention has the following beneficial effects:
(1) the components of the infant antibacterial biological detergent provided by the invention are nontoxic or extremely low in toxicity, have no stimulation to skin, are safe to use, have low residue, do not threaten the health of infants, and have light tea oil fragrance, the used nano degraded chitosan and polyaspartic acid can soften and make fabrics skin-friendly, and when the nano degraded chitosan and polyaspartic acid are used as a clothes detergent, the nano degraded chitosan and inactivated probiotics can prevent clothes of infants from hardening and rubbing the skin after being washed, and can protect the skin, so that the incidence rate of eczema and dermatitis of infants is reduced;
(2) the infant antibacterial biological detergent provided by the invention can reduce the surface tension of water, has rich and fine foam, strong detergency, strong hard water resistance and good solubility, has good killing and inhibiting effects on various bacteria and moulds, can ensure the activity of protease in the storage and application processes of the detergent, has a good removing effect on most common milk stains, sweat stains and the like on infant products, can be applied to cleaning of infant clothes, and can also be applied to cleaning of infant tableware, toys and other products;
(3) according to the infant antibacterial biological detergent provided by the invention, the tea saponin water-extraction fermentation liquor and the soapberry water-extraction fermentation liquor have high purity of the tea saponin and the soapberry saponin, and also contain the glycolipid surfactant and the antibacterial active substance, so that the detergent has good detergency and antibacterial activity, is low in cost and safe, can be rapidly fermented and decomposed, and cannot cause any pollution to the environment;
(4) the preparation method of the infant antibacterial biological detergent is simple to operate, clearly limits the feeding sequence, ensures the uniformity and stability of the detergent, and avoids the layering phenomenon.
Detailed Description
To further illustrate the technical measures taken by the present invention and the effects thereof, the following detailed description is given with reference to preferred embodiments of the present invention.
Example 1
An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 2 parts of tea saponin water extraction fermentation liquor, 3 parts of soapberry water extraction fermentation liquor, 5 parts of glycolipid biosurfactant, 5 parts of nano degraded chitosan, 0.05 part of polyaspartic acid, 0.57 part of arginine, 0.3 part of coco glucoside, 5 parts of encapsulated protease microcapsule, 2 parts of inactivated probiotics and 45 parts of deionized water.
Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.
Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.
Further, the glycolipid biosurfactant is rhamnolipid or sophorolipid.
Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.
Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.
A preparation method of an infant antibacterial biological detergent comprises the following steps:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.
Example 2
An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 5 parts of tea saponin water extraction fermentation liquor, 6 parts of soapberry water extraction fermentation liquor, 8 parts of glycolipid biosurfactant, 8 parts of nano degraded chitosan, 0.08 part of polyaspartic acid, 1.68 parts of arginine, 2.5 parts of coco glucoside, 8 parts of encapsulated protease microcapsule, 3 parts of inactivated probiotics and 52 parts of deionized water.
Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.
Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.
Further, the glycolipid biosurfactant is a trehalose glycolipid.
Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.
Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying sodium alginate solution in liquid paraffin containing span80Stirring at a warm temperature to form emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.
A preparation method of an infant antibacterial biological detergent comprises the following steps:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.
Example 3
An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 5 parts of tea saponin water extraction fermentation liquor, 6 parts of soapberry water extraction fermentation liquor, 8 parts of glycolipid biosurfactant, 8 parts of nano degraded chitosan, 0.08 part of polyaspartic acid, 2.49 parts of arginine, 3.4 parts of coco glucoside, 8 parts of encapsulated protease microcapsule, 3 parts of inactivated probiotics and 52 parts of deionized water.
Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.
Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.
Further, the glycolipid biosurfactant is a trehalose glycolipid.
Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.
Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.
A preparation method of an infant antibacterial biological detergent comprises the following steps:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.
Example 4
An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 7 parts of tea saponin water extraction fermentation liquor, 8 parts of soapberry water extraction fermentation liquor, 10 parts of glycolipid biosurfactant, 10 parts of nano degraded chitosan, 0.1 part of polyaspartic acid, 3.02 parts of arginine, 7 parts of coco glucoside, 10 parts of encapsulated protease microcapsule, 4 parts of inactivated probiotics and 65 parts of deionized water.
Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.
Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.
Further, the glycolipid biosurfactant is rhamnolipid, cellobiolipid and mannosylerythritol lipid.
Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.
Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.
A preparation method of an infant antibacterial biological detergent comprises the following steps:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.
Stability test
The detergents prepared in examples 1 to 4 were subjected to stability tests according to national standard GB9985-200, and the experimental results are shown in Table 1.
TABLE 1 stability test results
| Temperature of | Example 1 | Example 2 | Example 3 | Example 4 |
| -5℃ | Without delamination | Without delamination | Without delamination | Without delamination |
| 25℃ | Without delamination | Without delamination | Without delamination | Without delamination |
| 40℃ | Without delamination | Without delamination | Without delamination | Without delamination |
As can be seen from Table 1, the antibacterial biological detergents for infants prepared in examples 1 to 4 have no delamination at-5 ℃, 25 ℃ and 40 ℃, which indicates that the detergents provided by the invention have stable states at various temperatures.
Routine index detection
The results of the tests conducted with reference to the national standards for the requirements of hand dishwashing detergents are shown in Table 2.
TABLE 2 results of conventional index detection
As can be seen from Table 2, the infant antibacterial bio-detergent prepared in the examples 1-4 does not contain fluorescent agent, methanol, formaldehyde and other harmful substances, and meets the national standard and middle index requirements of tableware detergent.
Comparative example 1
The procedure is as described in example 3, except that the glycolipid biosurfactant is not included.
Comparative example 2
The procedure is as in example 3 except that polyaspartic acid is not contained.
Comparative example 3
The procedure is as in example 3 except that the probiotic is not inactivated.
Performance testing
The following performance tests were performed on the detergents obtained in examples 1 to 4 and comparative examples 1 to 3, respectively, and the test methods and results were as follows:
1 foam Property measurement
The measurement was carried out according to the method for measuring foaming power of detergent in GB/T13173-2008 (Ross-Mile method). Hard water formulations are described in GB/T6367-1997. The foaming ability and foam stability of the test specimens were determined by measuring the foam height (mm) at 0, 5 and 10 min. The test conditions were w (detergent) 0.2% in aqueous solution, water hardness 250 and 150mg/L, respectively, and temperature (40 ± 0.5) ° c. The measurement results are shown in Table 3.
TABLE 3 foam Performance test results
As can be seen from Table 3, the water hardness is 250 and 150mg/L respectively, the temperature is (40 +/-0.5) DEG C, the foam height of the examples 1-4 is greater than 110mm at 0min, and the foam height is still more than 90mm at 10min, which shows that the detergent provided by the invention has good foamability, the foam fading is slow, and the foam performance is hardly influenced by the change of the water hardness. Comparative example 1 contains no glycolipid biosurfactant, and compared with example 3, the foam height is greatly reduced and the foam fading is faster, compared with example 3, which shows that the glycolipid biosurfactant has synergistic effect with tea saponin water extraction fermentation liquor and soapberry water extraction fermentation liquor in terms of foamability and foam fading rate. Comparative example 2 contains no poiyamenine, and compared to example 3, the foam height and fade were not significantly different from example 3, indicating that poiyamenine had no effect on the foam of the system. Comparative example 3 contained no inactivated probiotic bacteria, and compared to example 3, the foam height was slightly reduced and the foam regressed considerably, indicating that the inactivated probiotic bacteria had some effect on the system foam system.
2 detergency test
The determination is carried out according to the determination method of the detergency and the cycle washing performance of the washing agent for the clothing in GB/T13174-2008. Hard water formulations are described in GB/T6367-1997. The detergency of the samples was determined by measuring the washing conditions of the samples with respect to carbon black soiled cloth, protein soiled cloth and sebum soiled cloth. The test conditions were w (detergent) 0.2% in aqueous solution, water hardness 250 and 150mg/L, respectively, and temperature (40 ± 0.5) ° c. The measurement results are shown in Table 4.
TABLE 4 detergency test results
As can be seen from Table 4, the detergents obtained in examples 1 to 3 have good detergency on carbon black soiled cloth, protein soiled cloth and sebum soiled cloth, and the hardness of water has no influence on the detergency. Comparative example 1 does not contain glycolipid biosurfactant, and the detergency of the detergent is reduced compared with that of example 3, which shows that the glycolipid biosurfactant has a synergistic effect with tea saponin water extraction fermentation liquor and soapberry saponin water extraction fermentation liquor; comparative example 2 contains no polyaspartic acid, and the detergency is slightly reduced, indicating that polyaspartic acid has a promoting effect on the detergency efficacy of the surfactant in addition to the polysuppleness efficacy; comparative example 3, which contained no inactivated probiotic bacteria, had a slight decrease in detergency, probably due to the presence of detersive active ingredients in the metabolites contained in the live probiotic bacteria.
3 bacteriostatic test
According to QB/T2738-. Respectively adopting stock solution and solution with the mass fraction of 1%, acting for 20min, respectively determining the bacteriostatic rate of the detergent on staphylococcus aureus and escherichia coli by a suspension quantitative method, repeating the experiment for 3 times, and taking the average value as the final result, wherein the result is shown in table 5.
TABLE 5 results of the bacteriostatic test
As can be seen from Table 5, the detergents obtained in examples 1 to 3 have good bacteriostatic effects on Staphylococcus aureus and Escherichia coli, and have almost no influence on bacteriostatic activity after being diluted by 200 times. Comparative example 1 in addition to the absence of glycolipid biosurfactant, the bacteriostatic activity of the detergent was reduced compared to example 3, indicating that the glycolipid biosurfactant is closely related to the bacteriostatic activity of the detergent; comparative example 2 contains no polyaspartic acid, and the bacteriostatic activity is slightly reduced compared with example 3, which shows that polyaspartic acid plays an important role in the aspect of good bacteriostatic activity of the detergent; comparative example 3 contains no inactivated probiotic bacteria and the bacteriostatic activity is also reduced, probably because the bacteriostatic metabolic products contained in the inactivated probiotic bacteria play a key role in the bacteriostatic activity of the detergent.
In conclusion, the components of the baby antibacterial biological detergent provided by the invention have synergistic effect, the foamability is good, the foam retention time is long, the effect of uniformly and well removing various stains is achieved, the antibacterial biological detergent is strong in antibacterial activity, and the baby antibacterial biological detergent can be directly used as a baby product detergent or applied to baby tableware, toys and clothes detergent products.
The above description is only for the specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and other modifications or equivalent substitutions made by the technical solution of the present invention by the ordinary skilled in the art should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (2)
1. An antibacterial biological detergent for infants is characterized by comprising the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water;
the glycolipid biosurfactant is one or more of rhamnolipid, sophorolipid, trehalose glycolipid, cellobiose and mannose erythritol lipid; the particle size of the nano degradable chitosan is 100-500 nm; the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio is 2:2: 1;
the preparation method of the tea saponin aqueous extract fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth;
the preparation method of the soapberry water extraction fermentation liquid comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain soapberry water extract fermentation liquor;
the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl2And (3) dripping the mixture into the emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after dripping, demulsifying and layering by using isopropanol, performing suction filtration, and washing by using distilled water for multiple times to obtain the microcapsule.
2. A method of preparing an infant antimicrobial biobased detergent according to claim 1, comprising the steps of:
step one, taking raw materials according to the weight part ratio;
dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;
and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco-glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH, and continuously uniformly stirring to obtain the product.
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| CN111304015A (en) * | 2020-03-04 | 2020-06-19 | 广州衣鸣日化科技有限公司 | Antibacterial high-concentration laundry detergent and preparation method thereof |
| CN113304105A (en) * | 2021-06-16 | 2021-08-27 | 江南大学 | Quaternary ammonium salt washing-free disinfection gel with skin mildness and preparation method thereof |
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| CN116509827A (en) * | 2023-04-28 | 2023-08-01 | 天津大学 | A kind of cleaning type skin disinfectant and its application |
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Denomination of invention: A baby antibacterial biological detergent and its preparation method Granted publication date: 20210202 Pledgee: Guangzhou Baiyun Branch of China Construction Bank Co.,Ltd. Pledgor: Guangdong Jumei daily necessities Co.,Ltd. Registration number: Y2024980040825 |
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