CN109336995A - A method of extracting chitosan from Pleurotus eryngii - Google Patents
A method of extracting chitosan from Pleurotus eryngii Download PDFInfo
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- CN109336995A CN109336995A CN201811554773.9A CN201811554773A CN109336995A CN 109336995 A CN109336995 A CN 109336995A CN 201811554773 A CN201811554773 A CN 201811554773A CN 109336995 A CN109336995 A CN 109336995A
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- chitosan
- pleurotus eryngii
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 58
- 244000252132 Pleurotus eryngii Species 0.000 title claims abstract description 34
- 235000001681 Pleurotus eryngii Nutrition 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 19
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 36
- 239000000243 solution Substances 0.000 claims abstract description 24
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 17
- 238000010992 reflux Methods 0.000 claims abstract description 17
- 230000008961 swelling Effects 0.000 claims abstract description 13
- 239000002253 acid Substances 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 12
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 238000005119 centrifugation Methods 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 238000000498 ball milling Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000009826 distribution Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract 2
- 239000000284 extract Substances 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 229920001410 Microfiber Polymers 0.000 description 2
- 239000003658 microfiber Substances 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 1
- 244000234623 Coprinus comatus Species 0.000 description 1
- 235000004439 Coprinus comatus Nutrition 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012668 chain scission Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
A kind of method for extracting chitosan from Pleurotus eryngii provided by the invention, including Pleurotus eryngii is pre-processed into powder processed, it is added in the mixed solution of NaOH solution and isopropanol, is stirred at reflux into powder raw material, filter, washing obtains chitosan swelling body；Chitosan swelling body is mixed with mixed acid solution again, is stirred at reflux；Finally using suspension is obtained after ultrasonic cleaner ultrasonic treatment, centrifuge washing obtains chitosan after dry.Not only content is high for the chitosan that the method that the present invention extracts chitosan from Pleurotus eryngii obtains, quality, and products obtained therefrom narrow molecular weight distribution, molecular weight are low.
Description
Technical field
The present invention relates to a kind of extracting methods of chitosan, and in particular to a kind of side that chitosan is extracted from Pleurotus eryngii
Method.
Background technique
Chitosan is the deacetylated product of chitin, the unique a large amount of existing alkaline polysaccharides of nature, due to having life
Object compatibility, biodegradable, without side effect and natural broad-spectrum antiseptic power etc., therefore be widely used in agricultural, medicine, food
And the fields such as weaving.In industrial production, chitosan is mainly using shrimp, crab shell as raw material, but the chitosan extracted has de- second
The defects of acyl degree is unstable.Research finds that the content of chitosan in fungal cell wall is very considerable, and the product matter extracted
It measures higher.
The study found that chitosan can reduce the intake of heat and triglycerides accumulates in liver, and can press down
Blood lipid level processed, the especially chitosan in Pleurotus eryngii, protein, ash content, arsenic equal size are lower, are different from shrimp crab
The chitosan in shell source.Therefore, Pleurotus eryngii chitosan has the function of preventing fatty liver formation.In addition, Pleurotus eryngii and other one
As the difference of oyster mushroom, mushroom, coprinus comatus etc. of kind be: the time saved after tissue tight, high resilience, picking more general mushroom
It grows, and the yield of factory edible fungi Pleurotus eryngii also increased rapidly in recent years.Therefore, chitosan is extracted from Pleurotus eryngii is
An important channel in chitosan source at present.
Summary of the invention
For above content in the prior art, the object of the present invention is to provide one kind, and higher quality is extracted from Pleurotus eryngii
And the method for the chitosan of higher amount.
In order to achieve the above object, the invention provides the following technical scheme:
A method of extracting chitosan from Pleurotus eryngii, comprising the following steps:
S1: ethanol solution is added in appropriate Pleurotus eryngii, reflux pretreatment, crushed after being dried, ball milling system are carried out to mixture
Powder；
S2: it is added in the powder obtained in step sl in the mixed solution of NaOH solution and isopropanol, at 60-80 DEG C
It is stirred at reflux, then filters, be washed to neutrality with distillation and obtain chitosan swelling body；
S3: chitosan swelling body obtained in step S2 is mixed with 40-50% mixed acid solution, at 100-110 DEG C
It is stirred at reflux 2h；
S4: the mixed liquor of S3 being ultrasonically treated after 2h in ultrasonic cleaner and obtains suspension, is washed to after centrifugation
Property, chitosan is obtained after dry.
In a preferred embodiment, further include in step S4, by the S3 and S4 back flow reaction step and
Sonicating step repeats 3-4 times, obtains suspension, and neutrality is washed to after centrifugation, obtains chitosan after dry.
In a preferred embodiment, further include step S5: the obtained chitosan of step S4 being purified, specifically
Are as follows: sodium hydroxide is added and is uniformly mixed, 20-30min is impregnated at a temperature of 40-50 DEG C, neutrality is washed to after centrifugation, is obtained after dry
To the chitosan of high-purity.
In a preferred embodiment, in step S1, the Pleurotus eryngii powder of 40 mesh obtained after ball milling.
In a preferred embodiment, in step S2, NaOH solution and isopropanol are added into Pleurotus eryngii powder
In mixed solution, 4-5h is stirred at reflux at 80 DEG C.
In a preferred embodiment, in step S3, chitosan swelling body obtained in step S2 is mixed with 40%
Acid solution mixing is closed, is stirred at reflux 2h at 110 DEG C；Wherein, the mixed acid solution be volume ratio be 2:1 the concentrated sulfuric acid with it is dense
Hydrochloric acid mixed solution.
Pleurotus eryngii is first carried out pretreatment powder processed by a kind of method that chitosan is extracted from Pleurotus eryngii of the invention, then will
The mixed solution of powder raw material and NaOH solution and isopropanol flows back.Since chitosan is existed in the form of microfibre
, and microfibre is mainly made of micromeritics and amorphous area two parts, the chitosan using isopropanol as dispersing agent, in Pleurotus eryngii
After alkali process, swelling body is formed, keeps its crystal structure loose, easily penetrates solvent, the acid being then added keeps part poly-
Close object chain scission of link, chitosan of gradually degrading, to obtain the chitosan particle of nano-scale.The present invention uses ultrasonic degradation shell
Glycan, reaction is mild, the narrow molecular weight distribution for the chitosan that can be carried out at low temperature, and obtain, and molecular weight is low.
Specific embodiment
Technical solution of the present invention is clearly and completely described below, it is clear that described embodiment is only this
Invention a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art exist
Every other embodiment obtained under the premise of creative work is not made, shall fall within the protection scope of the present invention.
A kind of embodiment 1: method for extracting chitosan from Pleurotus eryngii of the present embodiment, comprising the following steps:
S1: ethanol solution is added in appropriate Pleurotus eryngii, reflux pretreatment, crushed after being dried, ball milling system are carried out to mixture
Powder；Wherein, the Pleurotus eryngii powder of 40 mesh obtained after ball milling；
S2: it is added in the powder obtained in step sl in the mixed solution of NaOH solution and isopropanol, is stirred at 60 DEG C
Reflux 5h is mixed, is then filtered, is washed to neutrality with distillation and obtains chitosan swelling body；
S3: chitosan swelling body obtained in step S2 is mixed with 50% mixed acid solution, is stirred at reflux at 100 DEG C
2h；Wherein, above-mentioned mixed acid solution is the concentrated sulfuric acid and concentrated hydrochloric acid mixed solution that volume ratio is 2:1；
S4: being ultrasonically treated 2h for the mixed liquor of S3 in ultrasonic cleaner, and repeats returning in above-mentioned S3 and S4 again
Stream reaction step and sonicating step are repeated 3 times, after obtain suspension, be washed to neutrality after centrifugation, obtain shell after dry
Glycan；
S5: the obtained chitosan of step S4 is purified, specifically: sodium hydroxide is added and is uniformly mixed, 40 DEG C of temperature
Degree is lower to impregnate 30min, and neutrality is washed to after centrifugation, obtains the chitosan of high-purity after dry.
A kind of embodiment 2: method for extracting chitosan from Pleurotus eryngii of the present embodiment, comprising the following steps:
S1: ethanol solution is added in appropriate Pleurotus eryngii, reflux pretreatment, crushed after being dried, ball milling system are carried out to mixture
Powder；Wherein, the Pleurotus eryngii powder of 40 mesh obtained after ball milling.
S2: it is added in the powder obtained in step sl in the mixed solution of NaOH solution and isopropanol, is stirred at 80 DEG C
Reflux 4h is mixed, is then filtered, is washed to neutrality with distillation and obtains chitosan swelling body；
S3: chitosan swelling body obtained in step S2 is mixed with 40% mixed acid solution, is stirred at reflux at 110 DEG C
2h；Wherein, above-mentioned mixed acid solution is the concentrated sulfuric acid and concentrated hydrochloric acid mixed solution that volume ratio is 2:1；
S4: being ultrasonically treated 2h for the mixed liquor of S3 in ultrasonic cleaner, and repeats returning in above-mentioned S3 and S4 again
Stream reaction step and sonicating step are repeated 3 times, after obtain suspension, be washed to neutrality after centrifugation, obtain shell after dry
Glycan；
S5: the obtained chitosan of step S4 is purified, specifically: sodium hydroxide is added and is uniformly mixed, 40 DEG C of temperature
Degree is lower to impregnate 30min, and neutrality is washed to after centrifugation, obtains the chitosan of high-purity after dry.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. a kind of method for extracting chitosan from Pleurotus eryngii, it is characterised in that: the following steps are included:
S1: ethanol solution is added in appropriate Pleurotus eryngii, reflux pretreatment, crushed after being dried, ball powder-grinding are carried out to mixture；
S2: it is added in the powder obtained in step sl in the mixed solution of NaOH solution and isopropanol, is stirred at 60-80 DEG C
Reflux, then filters, is washed to neutrality with distillation and obtains chitosan swelling body；
S3: chitosan swelling body obtained in step S2 is mixed with 40-50% mixed acid solution, is stirred at 100-110 DEG C
Flow back 2h；
S4: the mixed liquor of S3 being ultrasonically treated after 2h in ultrasonic cleaner and obtains suspension, is washed to neutrality after centrifugation, is done
Chitosan is obtained after dry.
2. a kind of method for extracting chitosan from Pleurotus eryngii according to claim 1, it is characterised in that: in step S4 also
Including, by the S3 and S4 back flow reaction step and sonicating step repeat 3-4 times, suspension is obtained, after centrifugation
It is washed to neutrality, obtains chitosan after dry.
3. a kind of method for extracting chitosan from Pleurotus eryngii according to claim 1, it is characterised in that: further include step
S5: the obtained chitosan of step S4 is purified, specifically: sodium hydroxide is added and is uniformly mixed, is soaked at a temperature of 40-50 DEG C
20-30min is steeped, neutrality is washed to after centrifugation, obtains the chitosan of high-purity after dry.
4. a kind of method for extracting chitosan from Pleurotus eryngii according to claim 1, it is characterised in that: in step S1,
The Pleurotus eryngii powder of 40 mesh obtained after ball milling.
5. a kind of method for extracting chitosan from Pleurotus eryngii according to claim 1, it is characterised in that: in step S2,
It is added into Pleurotus eryngii powder in the mixed solution of NaOH solution and isopropanol, is stirred at reflux 4-5h at 80 DEG C.
6. a kind of method for extracting chitosan from Pleurotus eryngii according to claim 1, it is characterised in that: in step S3,
Chitosan swelling body obtained in step S2 is mixed with 40% mixed acid solution, is stirred at reflux 2h at 110 DEG C；Wherein, institute
Stating mixed acid solution is the concentrated sulfuric acid and concentrated hydrochloric acid mixed solution that volume ratio is 2:1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811554773.9A CN109336995A (en) | 2018-12-19 | 2018-12-19 | A method of extracting chitosan from Pleurotus eryngii |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811554773.9A CN109336995A (en) | 2018-12-19 | 2018-12-19 | A method of extracting chitosan from Pleurotus eryngii |
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| CN109336995A true CN109336995A (en) | 2019-02-15 |
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| CN201811554773.9A Pending CN109336995A (en) | 2018-12-19 | 2018-12-19 | A method of extracting chitosan from Pleurotus eryngii |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111533824A (en) * | 2020-06-08 | 2020-08-14 | 颜如玉医药科技有限公司 | Preparation method of agaricus bisporus chitin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104926957A (en) * | 2015-06-26 | 2015-09-23 | 广西大学 | Method of extracting polysaccharides from Pleurotus eryngii |
| CN108659145A (en) * | 2018-06-19 | 2018-10-16 | 苏州汉德瑞生物工程有限公司 | The method of extraction fungi chitosan and application in a kind of mushroom |
| CN108991531A (en) * | 2018-08-09 | 2018-12-14 | 合肥福泉现代农业科技有限公司 | A kind of method that Polysaccharide in Pleurotus eryngii collaboration chitosan prepares edible film |
-
2018
- 2018-12-19 CN CN201811554773.9A patent/CN109336995A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104926957A (en) * | 2015-06-26 | 2015-09-23 | 广西大学 | Method of extracting polysaccharides from Pleurotus eryngii |
| CN108659145A (en) * | 2018-06-19 | 2018-10-16 | 苏州汉德瑞生物工程有限公司 | The method of extraction fungi chitosan and application in a kind of mushroom |
| CN108991531A (en) * | 2018-08-09 | 2018-12-14 | 合肥福泉现代农业科技有限公司 | A kind of method that Polysaccharide in Pleurotus eryngii collaboration chitosan prepares edible film |
Non-Patent Citations (4)
| Title |
|---|
| ERYNGIIDUODUO XU等: "Charaterization and immunomodulatory activities of polysaccharideisolated from Pleurotus eryngii", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
| ZHENZHEN REN等: "Anti-hyperlipidemic and antioxidant effects of alkali-extractablemycelia polysaccharides by Pleurotus eryngii var. tuolensis", 《CARBOHYDRATE POLYMERS》 * |
| 张丹慧等: "贵金属-石墨烯纳米复合材料的合成及性能", 《贵金属-石墨烯纳米复合材料的合成及性能》 * |
| 陈若云等: "中国食用药用真菌化学", 《中国食用药用真菌化学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111533824A (en) * | 2020-06-08 | 2020-08-14 | 颜如玉医药科技有限公司 | Preparation method of agaricus bisporus chitin |
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Application publication date: 20190215 |