CN109276759A - A biomimetic antibacterial abdominal wall repair material - Google Patents

Abstract

The invention discloses a biomimetic antibacterial abdominal wall repair material. The abdominal wall repair material comprises a porcine small intestine submucosa and a chitosan-elastin fiber mesh layer coated on the porcine small intestine submucosa; and the chitosan-elasticity The protein fiber mesh layer is an electrospinning nano-scale chitosan-elastin fiber mesh. In the present invention, the antibacterial ability of the abdominal wall repair material is effectively improved by combining the chitosan-elastin fibrous mesh layer with the porcine small intestine submucosa membrane; at the same time, the mechanical strength and degradation performance of the abdominal wall repair material meet the requirements of abdominal wall repair. According to the requirements, it can also promote angiogenesis and facilitate tissue regeneration, help to solve the existing technical bottleneck of abdominal wall repair materials, and effectively improve the therapeutic effect of abdominal wall defect repair.

Description

A biomimetic antibacterial abdominal wall repair material

technical field

The invention belongs to the technical field of medical consumables, and in particular relates to a bionic antibacterial abdominal wall repair material.

Background technique

The development and application of abdominal wall repair materials is an important direction of current hernia and abdominal wall surgery research. The current abdominal wall repair patch still has some problems that need to be solved urgently. Their main disadvantage remains low biological activity, resulting in poor vascularization and uneven cellularization of the implant itself, ultimately resulting in a lack of integration with surrounding tissues and tissue regeneration. The recent use of acellular matrices in abdominal wall repair represents an effort to develop biomimetic grafts characterized by higher biocompatibility and improved in vivo performance. After implantation, the graft undergoes one of the following fates: (i) neovascularization and new ECM deposition; (ii) fibrotic encapsulation; (iii) resorption/degradation. Therefore, ideal grafts should favor early neovascularization, be fully biocompatible, and biomimetic to promote regenerative immune responses, rather than inflammation that can lead to fibrotic encapsulation. Furthermore, it should allow the recruitment of stem cells and adult tissue-specific cells to ultimately restore tissue function. Some scholars have studied biologically inspired methods of bionic patches for abdominal wall reconstruction.

This clinical need is addressed using the overarching principles of biomimicry to create materials that better resemble the composition, structure, and mechanical properties of target tissues. Collagen and elastin are the most abundant components of the extracellular matrix (ECM) of nearly all tissues in the body, providing essential cues for cell attachment, migration, and organization. Collagen and elastin are also two major components of the extracellular matrix of the abdominal wall, and changes in their ratio or metabolism are associated with the occurrence of abdominal wall hernias. Decellularized porcine intestinal submucosa is a natural biomaterial rich in active factors, mainly composed of collagen and some growth factors and signal molecules. It has excellent biological activity and is used in the repair of various tissue defects. The biological advantage of the tissue structure and function can be obtained. Elastin provides elasticity and deformability to tissues. The properties of elastin are critical for specific tissue functions (eg, dermis, blood vessels, muscle). Furthermore, some evidence suggests that soluble elastin facilitates tissue regeneration by promoting angiogenesis. Therefore, porcine small intestinal submucosa and elastin composites can be used for biomimetic repair of abdominal wall structure and composition.

There is an urgent need for a hernia repair material that can be actively antibacterial and can be applied to contaminated and infected wounds. Abdominal wall defects under the condition of pollution and infection are very common in combat trauma, traffic injury, and intestinal surgery at the same time. At present, most of these abdominal wall defects are debridement in one stage, and then repaired with materials in the second stage. It not only prolongs the treatment time and causes long-term pain to patients, but also causes the loss of health economy and social labor force. To solve the above problems, it is necessary to introduce antibacterial materials into abdominal wall repair materials. Chitosan is a kind of degradable material, which belongs to natural polycationic biopolysaccharide and has broad-spectrum antibacterial effect. There are two main viewpoints on the antibacterial mechanism of chitosan. One is a mechanism based on the polycationic properties of chitosan, which targets the negatively charged cell membrane of bacteria. Make it dented, deformed and leaked; another antibacterial mechanism is based on the DNA in the bacterial molecule as the target, which points out that chitosan enters the cell after passing through the damaged cell wall, and further interacts with the cytoplasm to disrupt the transcription of bacterial DNA molecules. Chitosan also has good biocompatibility and biodegradability, which can significantly improve the proliferation and migration speed of fibroblasts, promote the synthesis of extracellular matrix, and create a good healing environment for the defect site.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a bionic antibacterial abdominal wall repair material, which is suitable for hernia and abdominal wall surgical repair, and effectively improves the therapeutic effect of clinical abdominal wall defect repair.

In order to solve the above-mentioned technical problems, the present invention is specifically realized through the following technical solutions:

A biomimetic antibacterial abdominal wall repair material comprises a porcine small intestine submucosa and a chitosan-elastin fibrous mesh layer, wherein the chitosan-elastin fibrous mesh layer is coated on one side of the pig small intestine submucosa.

The chitosan-elastin fiber mesh layer is a nano-scale chitosan-elastin fiber mesh layer, and the fiber diameter of the polysaccharide-elastin fiber mesh layer is 80-230 nanometers, and the thickness is 50-300 nanometers. microns. The nano-scale chitosan-elastin fiber mesh is beneficial to better exert the antibacterial effect of the chitosan-elastin fiber layer on the repair effect of the porcine small intestine submucosa.

The chitosan-elastin fiber mesh layer is prepared by the method of electrospinning, and is combined with the submucosa membrane of the small intestine of pigs, which further optimizes the medical performance of the abdominal wall repair material.

The porcine small intestine submucosa is 4 layers of decellularized porcine small intestine submucosa material.

The beneficial effects of the present invention are:

In the invention, the chitosan-elastin fiber mesh layer is combined with the submucosa membrane of the small intestine of pigs, so that the obtained abdominal wall repair material has biomimetic properties, antibacterial properties and biological activity. At the same time, the mechanical strength and degradation performance of the abdominal wall repair material meet the requirements of abdominal wall repair, and can also promote angiogenesis to facilitate tissue regeneration, help to solve the existing technical bottleneck of abdominal wall repair materials, and effectively improve the therapeutic effect of abdominal wall defect repair.

Description of drawings

1 is a schematic structural diagram of a biomimetic antibacterial abdominal wall repair material in Example 1 of the present invention; wherein 1 is the submucosa of pig small intestine, and 2 is the chitosan-elastin fiber mesh layer;

Fig. 2 is the scanning electron microscope picture of the pig small intestine submucosa in Example 1 of the present invention, wherein Fig. 2 (a) is a low magnification, and Fig. 2 (b) is a high magnification;

3 is a cross-sectional view of the porcine small intestine submucosa in Example 1 of the present invention;

Fig. 4 is the scanning electron microscope image of the bionic antibacterial abdominal wall repair material in Example 1 of the present invention, wherein Fig. 4(a) is a low magnification, and Fig. 4(b) is a high magnification;

5 is a cross-sectional view of a bionic antibacterial abdominal wall repair material in Example 1 of the present invention.

Detailed ways

The technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

A biomimetic antibacterial abdominal wall repair material, as shown in Figure 1, comprises a porcine small intestine submucosa and a chitosan-elastin fibrous mesh layer coated on one side of the porcine small intestine submucosa.

The thickness of the above-mentioned chitosan-elastin fiber mesh layer is 50 microns, the fiber diameter of the nano-scale chitosan-elastin fiber is 100 nanometers, and the nano-scale chitosan-elastin fiber mesh is prepared by electrospinning And get. As shown in Fig. 2 and Fig. 3, the above-mentioned porcine small intestinal submucosa is a 4-layer decellularized porcine small intestinal submucosa material.

As shown in Figure 4 and Figure 5, the scanning electron microscope images of the obtained biomimetic antibacterial abdominal wall repair material are prepared. It can be seen from Figure 4 that the chitosan-elastin fibers in the biomimetic antibacterial abdominal wall repair material have a smooth surface, and the fiber diameter is on the order of nanometers; Figure 5 shows that the two layers of materials in the biomimetic antibacterial abdominal wall repair material are closely combined.

Example 2

A biomimetic antibacterial abdominal wall repair material, comprising a porcine small intestine submucosa and a chitosan-elastin fibrous mesh layer coated on one side of the porcine small intestine submucosa

In this embodiment, the thickness of the nano-scale chitosan-elastin fiber mesh layer is 100 microns, the fiber diameter of the nano-scale chitosan-elastin fiber is 150 nanometers, and the nano-scale chitosan-elastin fiber mesh is Prepared by electrospinning. The porcine small intestinal submucosa in this embodiment is a 4-layer decellularized porcine small intestinal submucosa material.

Example 3

A biomimetic antibacterial abdominal wall repair material comprises a porcine small intestine submucosa and a nano-scale chitosan-elastin fiber mesh layer coated on one side of the porcine small intestine submucosa.

In this embodiment, the thickness of the nano-scale chitosan-elastin fiber mesh layer is 150 microns, the fiber diameter of the nano-scale chitosan-elastin fiber is 200 nanometers, and the nano-scale chitosan-elastin fiber mesh is Prepared by electrospinning. The porcine small intestinal submucosa in this embodiment is a 4-layer decellularized porcine small intestinal submucosa material.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principle and spirit of the invention Variations, the scope of the invention is defined by the appended claims and their equivalents.

Claims (5)

1. a biomimetic antibacterial abdominal wall repair material, is characterized in that, comprises pig small intestine submucosa and chitosan-elastin fibrous mesh layer, and described chitosan-elastin fibrous mesh layer is coated on pig small intestine submucosa one. side. 2 . The bionic antibacterial abdominal wall repair material according to claim 1 , wherein the chitosan-elastin fiber mesh layer is a nano-scale chitosan-elastin fiber mesh layer. 3 . 3 . The bionic antibacterial abdominal wall repair material according to claim 2 , wherein the fiber diameter of the polysaccharide-elastin fiber mesh layer is 80-230 nanometers, and the thickness is 50-300 micrometers. 4 . 4 . The bionic antibacterial abdominal wall repair material according to claim 3 , wherein the chitosan-elastin fiber mesh layer is prepared by the method of electrospinning. 5 . 5 . The bionic antibacterial abdominal wall repair material according to claim 1 , wherein the porcine small intestine submucosa is 4 layers of acellular porcine small intestine submucosa material. 6 .