Have tobacco seedling medium of strong sprout and reduction Cd uptake and preparation method thereof and purposes
Technical field
The invention belongs to agriculture field, it is related to the cigarette for improving sound tobacco production, reducing tobacco leaf heavy metal cadmium (Cd) harm
Grass safety in production, and in particular to a kind of to have both strong sprout and reduce tobacco leaf to the Cd tobacco seedling medium absorbed and its preparation and its answer
Use technology.
Background technique
Culturing and transplanting seedlings are the tobacco cultivation methods that countries in the world carry out extensively, and nursery occupies important position in tobacco leaf production
It sets.Cultivating enough strong sprouts is to complete the precondition of leaf tobacco production plan, and obtain the basis of good quality and high output tobacco leaf.Mesh
Before, the plantation of tobacco is generallyd use after nutritive cube carries out floating seedlings and is transplanted, since the bowl of floating disc nursery is little, such as
Fruit seedling medium does not have plentiful nutrient, it usually needs constantly carries out top dressing.Currently, in tobacco seedling cultivation, seedling medium
The agricultural wastes compost fermentation product such as turf, domestic sludge compost, flyash is generallyd use, due to planting in above-mentioned seedling medium
Microbial activity etc. Shortcomings in harmony and matrix between the deficiency of object nutrient element content, nutrient element, thus
Influence the cultivation of strong sprout.In addition, survey data is shown, China plants smoke hallway Cd pollution exceeding standard rate and reaches 12.4%.Tobacco pair
Cd enriched character with higher in soil, Cd Pollution in Soil not only generate inhibition to the growth and development of tobacco, can also reduce cigarette
Leaf chemical quality.A large number of studies show that Cd Pollution in Soil has significant inhibiting effect to tobacco growing, Cd can inhibit green in tobacco leaf
Acid esters reductase and the synthesis of amino-Laevulinic Acid in element, cause tobacco leaf photosynthetic parameter and fluorescence parameter decline and chlorophyll a/
The reduction of b and carotenoid content, lead to tobacco leaf yellow chlorosis, and tobacco leaf weight per leaf is remarkably decreased;In addition, Cd passes through shadow
The metabolism of amino acid, transport activity in tobacco leaf are rung, and then influences tobacco leaf chemical quality, showing as sugar/alkali, nitrogen/alkali ratio cannot reach
The other index of quality of requirement and tobacco leaf to sound tobacco index it is uncoordinated;Cd to tobacco macronutrient (including K, Ca,
Mg, Si, Fe etc.) and the absorption of microelement (such as Zn, Se etc.) also have an impact, to influence the quality of tobacco, it is seen then that effectively drop
Low tobacco is of great significance to the absorption and transport technology of heavy metal (Cd) to the safety in production of less harmful tobacco.
Summary of the invention
In view of the drawbacks of the prior art, the purpose of the present invention is to provide a kind of tool strong sprouts educates with the tobacco for reducing Cd uptake
Seedling matrix and preparation method thereof and purposes.
To achieve the goals above, the invention adopts the following technical scheme:
A kind of tobacco seedling medium of tool strong sprout and reduction Cd uptake, the seedling medium includes wormcast and attapulgite
Powder.
In above-mentioned tobacco seedling medium, the wormcast and the attapulgite as a preferred implementation manner,
The weight ratio of powder are as follows: 10:1-1:1 (such as 9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1), it is highly preferred that the earthworm
The weight ratio of excrement and the attapulgite powder are as follows: 5:1-1:1, more preferably 3:1-2:1, further preferably 7:3.Two kinds of raw materials
Amount ratio to strong sprout and reduce maturity period tobacco leaf Cd uptake resultant effect have larger impact, in weight ratio range of the invention
It is interior to obtain ideal resultant effect.Inventor is by a large number of experiments discovery, the weight of wormcast and attapulgite powder
Resultant effect is ideal when than for 7:3.
In above-mentioned tobacco seedling medium, the granularity of the wormcast is not more than 1mm as a preferred implementation manner,
(such as 0.8mm, 0.5mm, 0.1mm, 0.01mm), preferably not greater than 0.2mm, and/or, the attapulgite Powder Particle Size is
(- 200 mesh, such as 250 mesh, 300 mesh, 350 mesh) below 200 mesh, it is preferable that the attapulgite Powder Particle Size is 300 mesh or less
(- 300 mesh).Wormcast carefully will increase very much cost of labor, is slightly unfavorable for very much the mixing with attapulgite powder, influences overall effect,
Attapulgite belongs to clay mineral, wherein not only containing the plant nutrients element such as K, Ca, Mg, Al, Si needed for plant, Er Qiezuo
For inorganic mineral colloid, particle is thinner, and colloid absorption specific surface is bigger, and effect is better, and the validity of nutrient element is also higher, but
It is, in order to control the preferred 200 mesh extracting screen underflow of mistake of cost.
In above-mentioned tobacco seedling medium, the seedling medium also includes nutrient solution as a preferred implementation manner,
Contain N, P in the nutrient solution2O5,K2O, Fe, B, Cu, Zn, Mo, Mn, S and Mg;Preferably, in the nutrient solution, N, P2O5,
K2The concentration of O, Fe, B, Cu, Zn, Mo, Mn, S and Mg are followed successively by 30mg/L, 15mg/L, 30mg/L, 0.10mg/L, 0.05mg/L,
0.02mg/L,0.02mg/L,0.01mg/L,0.05mg/L,0.05mg/L,0.05mg/L。
In above-mentioned tobacco seedling medium, the pH value of the nutrient solution is 6.0- as a preferred implementation manner,
6.5.Its pH has certain influence to tobacco seedlings development, and pH is too high or too low to generate inhibition to root system development.
In above-mentioned tobacco seedling medium, the nutrient solution is in the tobacco seedling as a preferred implementation manner,
Dosage in matrix is the maximum water-holding capacity for making the mixture of the wormcast and the attapulgite powder have 60-80%, excellent
Selection of land, dosage of the nutrient solution in the tobacco seedling medium are to make the mixing of the wormcast Yu the attapulgite powder
Object has 70% maximum water-holding capacity.
A kind of preparation method of above-mentioned tobacco seedling medium, the preparation method include: by wormcast and attapulgite powder
Mixing.
In the above preparation method, the preparation step of the wormcast includes: as a preferred implementation manner,
Compost maturity fermentation is carried out to cow dung, forms the product of decomposed fermentation;
With the product breeding earthworm of the decomposed fermentation;
Product to the decomposed fermentation is separated earthworm with wormcast by manual type after being eaten by earthworm, by
This obtains wormcast;
Optionally, it air-dries, obtains dried earthworms excrement;
Optionally, the dried earthworms excrement is sieved, obtains the satisfactory wormcast of granularity (i.e. no more than 1mm);And/or
The preparation step that the attapulgite crushes includes:
After attapulgite is tentatively crushed with Mechanical Method, ball-milling method is recycled to crush;
Optionally, smashed attapulgite is sieved, obtains the satisfactory attapulgite powder of granularity (- 200 mesh).
In the above preparation method, as a preferred implementation manner,
Above-mentioned nutrient solution is added into the mixture of the wormcast and the attapulgite powder, is stirred evenly;
Optionally, the additional amount of the nutrient solution is to have the wormcast and the mixture of the attapulgite powder
The maximum water-holding capacity of 60-80%,
Preferably, the additional amount of the nutrient solution is to have the wormcast and the mixture of the attapulgite powder
70% maximum water-holding capacity.
The preparation method of above-mentioned tobacco seedling medium or above-mentioned tobacco seedling medium is improving tobacco leaf weight and/or is reducing cigarette
Purposes in leaf in metal cadmium content.
In such use, as a preferred implementation manner,
The tobacco seedlings and its matrix cultivated using the tobacco seedling medium are transplanted in Cd contaminated soil jointly and are carried out
Plantation;Preferably, the content of Cd is 2-2.5mg/kg in the Cd contaminated soil, and the pH of the Cd contaminated soil is 6-7.
Beneficial effects of the present invention:
(1) this method are as follows: by carrying out compost maturity fermentation to cow dung, then cultivate earthworm with the cow dung after decomposed fermentation
Earthworm, obtains wormcast, then after wormcast is air-dried, obtains compost from earthworm after sieving;By attapulgite (clay mineral) ball
Mill method grinding and sieving is prepared into attapulgite;Then it is mixed thoroughly after mixing compost from earthworm, attapulgite according to a certain percentage.Match
The nutrient solution of seedling medium and floating pool processed, nutrient composition include the N of various concentration, P2O5,K2O,Fe,B,Cu,Zn,Mo,
Mn, S and Mg ingredient, with the H of 0.1mol/L2SO4Or the pH value of KOH solution adjustment nutrient solution is between 6.0-6.5, to wormcast
With maximum water-holding capacity (preferably 70% of the above-mentioned nutrient solution to 60-80% (preferably 70%) is added in attapulgite mixture
MWHC), nursery is carried out for tobacco seedling alms bowl after then stirring, after the end of nursery, carries out cigarette together with seedling medium
Transplantation of seedlings.The economical character of tobacco seedlings before transplanting can be made to greatly improve using the seedling medium in the present invention.With traditional seedling medium
Compare, before different seedling mediums of the invention can make to transplant tobacco seedlings stem girth, plant height and overground part fresh weight maximum increase by 19.9%,
25.9% and 57.3%;In addition, the SPAD value and root activity (ug/ root .h) of different seedling medium processing tobacco seedlings chlorophyll compare
Increase by 26.5% and 35.9% according to maximum.In addition to this, the maturity period tobacco leaf weight per leaf of different seedling medium processing is more maximum than control
75.5% is increased, Cd content maximum reduces by 72.0% in tobacco leaf.The present invention is low using prepared tobacco seedling medium centering
The production for producing tobacco field High Quality Tobacco and the production for planting less harmful tobacco leaf in cigarette district Cd contaminated soil have important value.
(2) wormcast is that the edible organic matter of earthworm is ground by earthworm gizzard, then passes through progress biology in earthworm body cavity
Excreta after being decomposed and converted after chemistry, microorganism synergistic effect, is the organic fertilizer that one kind has " life ".Earthworm enteron aisle
In containing a variety of biological enzyme such as protease, amylase, lipase, can be good at degradation of organic substances and generate good earthworm
Excrement.Wormcast has good physics, chemistry and biological nature, can not only be retained, fertilizer conservation, ventilation, and organic matter, plant
Object nutrient and microorganism equal size are very high, can significantly improve the growth and development of plant.Wormcast is compared with common organic fertilizer, no
Only have the characteristics that other organic fertilizers, and not to be that various organic fertilizers are simply mixed institute attainable for fertility effect, studies have shown that
Wormcast water conservation, venting capability are 1.5-2.0 times of general soil, and beneficial microbe is up to 200,000,000/gram soil.It can be fine
The relationship adjusted between soil, plant and microorganism, improve plant rhizosphere circle microenvironment, plant made to be finally reached disease-resistant, growth-promoting
Long purpose.The characteristics of wormcast is that edaphon, nutrient and active factors combine, firstly, wormcast is
A kind of solid particulate matter, porosity is larger, has good ventilative, water holding capacity, is very suitable to microorganism growth;Secondly, earthworm
Humic acid, organic matter, CEC, soluble-salt equal size are very high in earthworm excrement, are very beneficial for the growth and development of plant;Again, earthworm
Comprising the microorganism that there are many quantity, type in excrement, has wormcast due to the competition between microorganism and inhibit harmful bacteria breeding
Effect, to reduce the generation of pest and disease damage.As the clay mineral ingredient in matrix, attapulgite is a kind of by silicon oxygen four sides
The aqueous rich magnesium aluminosilicate clays mineral of the 2:1 type of body and alumina octahedral composition, variable amount of K is contained in crystal+、Ca2+、
Fe3+、Mg2+、Al3+Equal nutrients ion.The results show can make to transplant preceding tobacco seedlings using the tobacco seedling medium in the present invention
Economical character greatly improve.Compared with the control, different seedling mediums can be such that the stem girth (cm) of tobacco seedlings before transplanting increases
15.8%-19.9%, plant height (cm) increase 17.9%-25.9%, and overground part fresh weight increases 31.0%-57.3%;In addition, different
SPAD value and root activity (the ug/ root .h) of seedling medium processing tobacco seedlings compare compare increase separately 15.0%-26.5% and
27.4%-35.9%.Other than it can remarkably promote tobacco seedling growth, compared with the control, the tobacco leaf list of different seedling medium processing
Ye Chong increases 51.3%-75.5%, and Cd content has dropped 59.5%-72.0% respectively in tobacco leaf.In general, wherein with earthworm
Earthworm excrement/attapulgite=7:3 ratio processing matrix (T2) effect is best.
Detailed description of the invention
Fig. 1 difference seedling medium to the influence comparative diagram of tobacco leaf weight per leaf fresh weight (FW) (different letters indicate significant differences,
P<0.05)。
(different letters indicate that difference is aobvious to influence comparative diagram of Fig. 2 difference seedling medium to Cd content (mg/kg) in tobacco leaf
It writes, P < 0.05).
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention
Formula is described in further detail.
Embodiment 1: the preparation of tobacco seedling medium
Compost maturity fermentation is carried out to cow dung, then with the product breeding earthworm of decomposed fermentation, the cultivation of earthworm is using PVC
Slot (long * wide * depth=1.6 meters * 1.2 meters * 0.3 meter) carries out: the cow dung after decomposed fermentation early period being first layered on PVC slot about 15cm
Thickness, according to cow dung (air dry weight): water (weight ratio)=4:1 ratio sprays moisture, is then placed in the earthworm of about 6kg (fresh weight)
On cow dung, in order to keep appropriate aqueous amount, a small amount of moisture is sprayed every 1 circumferential breeding trough, (cow dung is substantially completely within about 45 days for cultivation
By feeding) after, the artificial earthworm separated in breeding trough obtains wormcast, then after wormcast is air-dried, takes under sieve after crossing 1-mm sieve
Object is as compost from earthworm;After attapulgite is tentatively crushed with Mechanical Method, ball-milling method is recycled to crush, takes sieve after crossing 200 meshes
Lower object is spare as concave convex rod pulvis;In this example, using wormcast and attapulgite according to three kinds of different weight ratios (w/w), system
For at 3 kinds of different seedling mediums, being respectively as follows: wormcast/attapulgite (w/w) is 6:4 (T1), wormcast/attapulgite (w/w)
It is 8:2 (T3) for 7:3 (T2) and wormcast/attapulgite (w/w).
Embodiment 2: the compounding in tobacco floating seed rearing pond
The preparation of nutrient solution is the demand characteristic according to tobacco to nutrition in tobacco seedling floating pool, is utilized respectively nitric acid
Ammonium, potassium dihydrogen phosphate, ferrous sulfate heptahydrate, cupric sulfate pentahydrate, white vitriol, potassium molybdate, manganese sulfate monohydrate, magnesium sulfate, boron
Acid, sulfuric acid etc. are dissolved in distilled water, prepare N, P2O5,K2The concentration (mg/L) of O, Fe, B, Cu, Zn, Mo, Mn, S and Mg are respectively 30,
15,30,0.10,0.05,0.02,0.02,0.01,0.05,0.05,0.05 nutrient solution, with the H of 0.1mol/L2SO4Or KOH is molten
Liquid adjusts the pH value of nutrient solution between 6.0-6.5.In this example, the preparation of nutrient solution is the demand and Zn according to tobacco to nutrition
To Cd agonist character.
Embodiment 3: effect experiment of the different substrates to tobacco seedling
(1) pre-treating method of tobacco seedling medium of the present invention is as follows: a) by the compost from earthworm for crossing 1-mm sieve of preparation
Grain is respectively that 6:4 (T1), 7:3 (T2) and 8:2 (T3) ratio mix according to weight ratio (w/w) with the attapulgite for crossing 200 meshes,
Stir, obtain the wormcast and attapulgite mixture of 3 kinds of different ratios, i.e. three kinds of seedling mediums (T1, T2 and
T3);B) according to " soil agrochemistry analysis method " (Lu Rukun, Beijing: Chinese agriculture Science Press, 2000) in record
Funnel method (funnel method is as follows), determine the maximum water-holding capacity (%) of wormcast Yu attapulgite mixture;C) according to step 2
The maximum water-holding capacity of measurement is as a result, add nutrient solution described in embodiment 2 into wormcast and attapulgite mixture to 70%
Maximum water-holding capacity (70%MWHC), it is stand-by after then stirring.
Wherein, the funnel method of the step b) in the pre-treating method of matrix, concrete operation step are as follows:
1) one layer of filter paper will be padded in the funnel of diameter 11cm, is connected under funnel with rubber tube, is clamped with clip;2) it weighs
50g baking substrate is fitted into funnel, takes 100ml water with graduated cylinder correct amount, and part water is added and is saturated into funnel to water supply in media,
Balance half an hour;3) rubber tube clip is opened, connects the water that matrix is leaked down with graduated cylinder;4) maximum water-holding capacity MWHC=100- filter paper is inhaled
The water volume that spills of water-.
The control matrix (CK) of tobacco seedling described in the present embodiment is derived from Hengyang, Hunan Province area field seedling raising and commonly educates
Seedling matrix, main component is the turfy soil after herbaceous plant compost fermentation, also according to the place of tobacco seedling medium of the present invention
Reason method carries out pre-treatment.
The main physical and chemical of the different seedling mediums of above-mentioned pre-treatment is not carried out referring to table 1.
The main physical and chemical of the different seedling mediums of table 1
Note: T1: wormcast/attapulgite (w/w) is 6:4;T2: wormcast/attapulgite (w/w) is 7:3;T3: earthworm
Excrement/attapulgite (w/w) is 8:2;The difference letter of same column indicates significant difference (P < 0.05), if after the data of same column
, which there is same letter in face then, indicates that difference is not significant, such as: there were significant differences by a and b, and ab and b are without significant difference, and ab and a are without significant
Difference, a and a are without significant difference, and b and b are without significant difference.
(2) tobacco seedling medium after using step (1) pre-treatment carries out nursery with the tobacco seedlings before being transplanted:
A) tobacco seed vernalization: 87 tobacco seed of cloud and mist is immersed in the Bordeaux mixture solution of 1:150 (V/V) 20 minutes
After take out, then seed is immersed in 20 DEG C of water 6-8 hours, is shelved on the filter paper of wetting, in 20-25 DEG C of vernalization 48-72
Hour, the sowing of pot for growing seedlings is carried out as Baconic length about 2-mm.
B) nursery is carried out using plastic seeding culturing plate, each plastic seeding culturing plate has 5 × 10=50 pot for growing seedlings, Mei Geyu respectively
The length of seedling alms bowl × wide × depth is respectively 4.5cm × 4.5cm × 5.0cm, and the low side of pot for growing seedlings has the leak hole of about diameter 2mm.
3 kinds of tobacco seedling mediums through step (1) pre-treatment and control matrix are respectively charged into different pot for growing seedlings, each
Plastic seeding culturing alms bowl sows the tobacco seed of 2 germinations, waits thinning after emergence in 7 days, and every alms bowl stays a tobacco seedling.Every kind of matrix treatments
It is repeated 15 times (15 nursery caves, wherein the tobacco seedlings that 10 nursery caves obtain are used for tobacco seedlings economical character measurement when culturing and transplanting seedlings, separately
Outer 5 cave tobacco seedlings are that 4 potted plant experiment of embodiment uses seedling), 60 nursery caves altogether.Pot for growing seedlings after planting is placed in plastics floating
Chi Zhong, in plastics pond plus nutrient solution to depth be about 2cm, by above-mentioned plastics floating pool be placed in greenhouse (25 ± 2 DEG C, natural light
According to) after culture 60 days, every kind of matrix treatments harvest 10 cave tobacco seedlings and carry out economical character, SPAD value and the measurement of root activity value, separately
Outer a part of tobacco seedlings are used for the potted plant experiment of embodiment 4.Different phase cultivation condition is referring to table 2.
Table 2 tests different disposal number
The measurement of SPAD value: it is measured using SPAD-502 chlorophyll meter.Selection number the down at the top of tobacco seedlings when measurement
The blade that three pieces are grown out completely, and take its average value.Root activity (ug/ root .h): the measurement of root activity uses chlorination three
Phenyl tetrazole (TTC) reduction method is measured.
Experimental result discovery can be such that the economical character of tobacco seedlings before transplanting substantially mentions using the tobacco seedling medium in the present invention
It is high.Compared with CK, the stem girth of tobacco seedlings increases 15.8%-19.9% in different seedling medium processing, and plant height increases 17.9%-
25.9%, overground part fresh weight increases 31.0%-57.3% (table 3).The SPAD value of different seedling mediums processing tobacco seedlings chlorophyll with
Root activity (ug/ root .h) increases separately 15.0%-26.5% and 27.4%-35.9% than control.
The economical character of tobacco seedlings before the different seedling medium processing of table 3 are transplanted
Note: the difference letter of same column indicates significant difference (P < 0.05)
Embodiment 4: different substrates are to the potted plant experiment for reducing tobacco Cd assimilation effect
Tobacco in this example is obtained after the tobacco seedlings that embodiment 3 obtains are carried out potting, and tobacco bred is cloud and mist 87.
The content of Cd is 2.16mg/kg in Cd contaminated soil used in potting described in this example, the Cd contaminated soil
PH is 6.75, soil moisture content 25%-30%.
It uses diameter for the plastic barrel of (25cm) × deep (30cm), adds water (distilled water) to 70% maximum Cd contaminated soil
It is stirred after field capacity, every barreled Cd contaminated soil (being based on air dry weight) 13.0kg, by warp above-mentioned in embodiment 3
The tobacco seedlings for crossing 60 days different disposals are transplanted together with culture substrate to each plastic barrel, one tobacco seedlings of every barrel of transplanting, each processing
It is repeated 3 times, shares 12 basins.Potted plant experiment carries out in plastic greenhouse (25 ± 2 DEG C of daytime, 20 ± 2 DEG C of night, natural lighting),
Naturally it is harvested after growing 90 days, acquisition carries out weight per leaf (fresh weight) measurement from the 8-17 piece leaf of lower part up number when harvest, will
Each processing is repeated to be averaged by the measurement result of the 8-17 piece leaf of every plant of tobacco three times again after being averaged, simultaneously
Tobacco leaf Cd assay is carried out to middle part tobacco leaf (several 9-12 piece leaves from the bottom up).
In this example, Cd assay is carried out referring to the standard method of GB/T17141 in soil;Cd assay is joined in tobacco
According to " soil agrochemistry analysis method " Lu Rukun, Beijing: the method in Chinese agriculture Science Press is measured.
Tobacco leaf biomass (weight per leaf) measurement result is found, in the tobacco leaf weight per leaf and tobacco leaf of different substrates nursery processing
Cd content has significant (P < 0.05) difference.In CK processing, mean univalent functions fresh weight is 42.5g, and different seedling mediums handle (T1-
T3 single leaf fresh weight) is 64.3g-74.6g (Fig. 1), increases 51.3%-75.5% than control.
To Cd assay in tobacco leaf, as a result, it has been found that, in control (CK, traditional matrices turfy soil) processing, tobacco leaf Cd content is
Cd content is respectively 2.47mg/kg, 1.92mg/kg and 2.78mg/kg in 6.86mg/kg, T1, T2, T3 processing tobacco leaf, than control
64.0%, 72.0% and 59.5% (Fig. 2) is had dropped respectively, wherein best to the effect for reducing Cd content in tobacco leaf with T2.Cause
This, prepared seedling medium T2 in the present invention can be used as to have both and cultivate tobacco strong sprout while reduce what tobacco absorbed Cd
A kind of especially desirable material.