CN109136091A - A kind of tablet culture medium and preparation method thereof - Google Patents
A kind of tablet culture medium and preparation method thereof Download PDFInfo
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- CN109136091A CN109136091A CN201811036236.5A CN201811036236A CN109136091A CN 109136091 A CN109136091 A CN 109136091A CN 201811036236 A CN201811036236 A CN 201811036236A CN 109136091 A CN109136091 A CN 109136091A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims description 16
- 239000002609 medium Substances 0.000 claims abstract description 54
- 239000000314 lubricant Substances 0.000 claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims description 26
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 24
- 229920002472 Starch Polymers 0.000 claims description 21
- 239000008107 starch Substances 0.000 claims description 21
- 235000019698 starch Nutrition 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 235000019359 magnesium stearate Nutrition 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 9
- 239000011734 sodium Substances 0.000 claims description 9
- 229910052708 sodium Inorganic materials 0.000 claims description 9
- 229920001223 polyethylene glycol Polymers 0.000 claims description 8
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims description 7
- 239000007884 disintegrant Substances 0.000 claims description 7
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 7
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 7
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 6
- 108010010803 Gelatin Proteins 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 229940014259 gelatin Drugs 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 239000008172 hydrogenated vegetable oil Substances 0.000 claims description 4
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 4
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 4
- 229940069328 povidone Drugs 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 3
- 229920002785 Croscarmellose sodium Polymers 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229960000913 crospovidone Drugs 0.000 claims description 3
- 229940031703 low substituted hydroxypropyl cellulose Drugs 0.000 claims description 3
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 claims description 3
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 claims description 3
- 235000010413 sodium alginate Nutrition 0.000 claims description 3
- 239000000661 sodium alginate Substances 0.000 claims description 3
- 229940005550 sodium alginate Drugs 0.000 claims description 3
- 235000010980 cellulose Nutrition 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 239000000454 talc Substances 0.000 claims description 2
- 229910052623 talc Inorganic materials 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- VJHCJDRQFCCTHL-UHFFFAOYSA-N acetic acid 2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)=O.OCC(O)C(O)C(O)C(O)C=O VJHCJDRQFCCTHL-UHFFFAOYSA-N 0.000 claims 1
- 229960005168 croscarmellose Drugs 0.000 claims 1
- 239000001767 crosslinked sodium carboxy methyl cellulose Substances 0.000 claims 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims 1
- 229940032147 starch Drugs 0.000 claims 1
- 239000000853 adhesive Substances 0.000 abstract description 14
- 230000001070 adhesive effect Effects 0.000 abstract description 14
- 230000002776 aggregation Effects 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000005054 agglomeration Methods 0.000 abstract description 3
- 239000000428 dust Substances 0.000 abstract description 3
- 206010020751 Hypersensitivity Diseases 0.000 abstract description 2
- 208000030961 allergic reaction Diseases 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 31
- 241000894006 Bacteria Species 0.000 description 21
- 230000012010 growth Effects 0.000 description 20
- 244000068988 Glycine max Species 0.000 description 16
- 235000010469 Glycine max Nutrition 0.000 description 16
- 229920001817 Agar Polymers 0.000 description 15
- 239000001888 Peptone Substances 0.000 description 15
- 108010080698 Peptones Proteins 0.000 description 15
- 239000008272 agar Substances 0.000 description 15
- 210000000496 pancreas Anatomy 0.000 description 15
- 235000019319 peptone Nutrition 0.000 description 15
- 239000000306 component Substances 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- CWERGRDVMFNCDR-UHFFFAOYSA-N thioglycolic acid Chemical class OC(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- 239000000725 suspension Substances 0.000 description 10
- 239000012530 fluid Substances 0.000 description 9
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- 241000191967 Staphylococcus aureus Species 0.000 description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 5
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 5
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 4
- 241000607762 Shigella flexneri Species 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
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- 238000005259 measurement Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- 239000004575 stone Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 230000001332 colony forming effect Effects 0.000 description 3
- 239000013065 commercial product Substances 0.000 description 3
- 239000008121 dextrose Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 238000012009 microbiological test Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- 241000193470 Clostridium sporogenes Species 0.000 description 2
- 206010067482 No adverse event Diseases 0.000 description 2
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229960001681 croscarmellose sodium Drugs 0.000 description 2
- 235000010947 crosslinked sodium carboxy methyl cellulose Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- YCSMVPSDJIOXGN-UHFFFAOYSA-N CCCCCCCCCCCC[Na] Chemical compound CCCCCCCCCCCC[Na] YCSMVPSDJIOXGN-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- -1 microelement) Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011076 safety test Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of tablet culture mediums, belong to culture medium technical field.The tablet culture medium includes the component of following parts by weight: 84~96 parts of dehydrated medium, 2~6 parts of adhesive, 2~8 parts of disintegrating agent and 0.5~2 part of lubricant.Tablet culture medium prepared by the present invention, quality are stablized, and the shortcomings that dehydrated medium is easy moisture absorption agglomeration is overcome.Meanwhile tablet culture medium prepared by the present invention can significantly reduce sucking of the user to toxic component, reduce allergic reaction, also pollution of the reduction dust to laboratory precision instrument and equipment.Tablet culture medium prepared by the present invention never degenerates, microculture works well for 3 years under room temperature, closed environment.
Description
Technical field
The invention belongs to microbiological culture media technical fields, and in particular to a kind of tablet culture medium and preparation method thereof.
Background technique
Microbiological culture media, the mixture of nutriment needed for referring to supply microbial growth, by Different Nutrition
Substance formulated in combination forms, generally all containing carbohydrate, nitrogen substance, inorganic salts (including microelement), vitamin and
Water etc..Culture medium had both been to provide cytotrophy and had promoted the basic substance of cell Proliferation and the existence of cell growth and breeding
Environment.Due to the uniqueness rich in nutriment and application, food and drug microbiological Test all to the preparation of culture medium,
Storage and quality control are proposed and are clearly required.
Early in four the fifties, American and Britain, Deng developed country largely use dehydrated medium, and become commercialization
Product.China also sets about developing in the fifties, and medicine unit is widely used now.Presently commercially available microbiological culture media is main
To be dehydrated synthetic media, it is divided into dehydrated medium and particulate media.
Dehydrated medium is in powdered, is by the various compositions of culture medium by proper treatment, according to various culture mediums
Formula aequum mixes well.Dehydrated medium is easy in moisture absorption agglomeration, transport and storage process easily dispersion unevenly, and
It is also easy to produce dust in use, a possibility that there are the environmental pollutions of clean area and there are security risks to operator.
To solve this problem, it is proposed granular pattern culture medium successively in the market, particulate media is the base in dried powder culture medium
By being granulated on plinth, have the advantages that soluble, non-dusting, be conducive to laboratory environment and personnel safety, by
The welcome and receiving of more and more users.But there is also some disadvantages for particulate media, such as pelletize unstable, culture medium
The problems such as capability and performance declines, protrusion are reflected in growth ability, Indication Characteristics of microorganism etc., not as good as dehydrated medium exhibition
The excellent culture performance revealed.And culture medium is the basis of microbiological test, directly affects microbial test results, unavoidably
There are the erroneous judgements of inspection result.
Summary of the invention
In view of this, the culture medium quality is stablized the present invention provides a kind of tablet culture medium, simplicity is used.
To solve the above-mentioned problems, the present invention provides following technical schemes:
The present invention provides a kind of tablet culture medium, the component including following parts by weight: 84~96 parts of dehydrated medium is glued
2~6 parts of mixture, 2~8 parts of disintegrating agent and 0.5~2 part of lubricant.
Preferably, the component including following parts by weight: 88~92 parts of dehydrated medium, 3~5 parts of adhesive, disintegrating agent 4~
6 parts and 1~1.5 part of lubricant.
Preferably, described adhesive includes: starch slurry, microcrystalline cellulose, povidone, gelatin, polyethylene glycol and alginic acid
One or more of sodium.
Preferably, the disintegrating agent includes: dried starch, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, the poly- dimension of crosslinking
One or more of ketone, croscarmellose sodium and gas-producing disintegrant.
Preferably, the lubricant includes: magnesium stearate, superfine silica gel powder, talcum powder, hydrogenated vegetable oil, polyethylene glycols
One or more of with lauryl sodium sulfate.
Preferably, the granularity of the dehydrated medium is 200~300 mesh.
Preferably, by weight percentage, the water content of each component stands alone as 8.0%.
The present invention provides a kind of preparation methods of tablet culture medium described in above scheme, include the following steps:
1) it by adhesive, disintegrating agent, lubricant and accounts for the dehydrated medium of dehydrated medium gross mass 15%~20% and mixes
It closes, obtains premix；
2) premix of the step 1) is mixed, tabletting with surplus dehydrated medium, obtains tablet culture medium.
Preferably, the step 1) or 2) in mix mode stand alone as stirring；The time of the stirring stands alone as 4~
The revolving speed of 6min, the stirring stand alone as 5~10r/min.
Preferably, the pressure of the tabletting is 39~41kN, and the diameter of tablet culture medium is 22mm.
The present invention provides a kind of tablet culture medium, the component including following parts by weight: 84~96 parts of dehydrated medium is glued
2~6 parts of mixture, 2~8 parts of disintegrating agent and 0.5~2 part of lubricant.Dehydrated medium provides required nutrition for microorganism growth
Ingredient and suitable pH environment etc., auxiliary material adhesive, disintegrating agent and lubricant are played a supporting role in tablet forming process,
Stability with higher, does not occur any physical-chemical reaction with major ingredient, nontoxic to microorganism, have no adverse reaction.Wherein, it sticks
Mixture makes no stickiness or the insufficient material powders of stickiness be gathered into particle, so that the further processing of particle is more easy.Disintegration
Agent can promote preparation to be disintegrated into junior unit rapidly, and then faster dissolve.The effect of lubricant is between reducing particle, particle and solid
Frictional force between preparation manufacturing equipment.Tablet culture medium prepared by the present invention will not cause to disperse in transport and storage process
Unevenly, to make unstable quality.The shortcomings that dehydrated medium is easy moisture absorption agglomeration is overcome simultaneously.Meanwhile system of the present invention
Standby tablet culture medium can significantly reduce sucking of the user to toxic component, reduce allergic reaction；Dust is reduced to laboratory
The pollution of precision instrument and equipment.And under room temperature, closed environment, never degenerate within 3 years, microculture works well.
Further, the present invention uses tablet, and the quality of tablet is fixed value, in use every be dissolved in it is quantitatively pure
Change in water, it is easy to operate without weighing.
Specific embodiment
The present invention provides a kind of tablet culture medium, the component including following parts by weight: 84~96 parts of dehydrated medium is glued
2~6 parts of mixture, 2~8 parts of disintegrating agent and 0.5~2 part of lubricant.
Tablet culture medium provided by the invention includes dehydrated medium.By weight, the dehydrated medium be 84~
96 parts, preferably 88~92 parts, more preferably 90 parts.In the present invention, the granularity of the dehydrated medium is preferably 200~300
Mesh, more preferably 250 mesh.In the present invention, the water content of commercially available dehydrated medium is preferably 7.5%~8.5%, more preferably
8%.Nutritional ingredient and suitable pH environment etc. needed for heretofore described dehydrated medium provides microorganism growth.This hair
The bright source category to the dehydrated medium is not particularly limited, drug involved in the existing pharmacopeia of this field conventional commercial
Examine all culture mediums involved in culture medium and food, cosmetics, drinking water Micro biological Tests current standard.
Tablet culture medium provided by the invention includes adhesive.By weight, described adhesive includes 2~6 parts, preferably
It is 3~5 parts, more preferably 4 parts.In the present invention, described adhesive preferably includes starch slurry, microcrystalline cellulose, povidone, bright
One or more of glue, polyethylene glycol and sodium alginate.In the present invention, the water content of the water content of described adhesive is preferably
7.5%~8.5%, more preferably 8%.In the present invention, described adhesive makes no stickiness or the insufficient material powders aggregation of stickiness
At particle, so that the further processing of particle is more easy.The present invention is not particularly limited the source of described adhesive, uses
This field conventional commercial product.Starch slurry, microcrystalline cellulose and povidone are bought from the Anhui mountains and rivers in the embodiment of the present invention
Pharmaceutic adjuvant limited liability company；Gelatin is bought from Hangzhou Qun Li gelatin Chemical Co., Ltd.；Polyethylene glycol is bought from Beijing
Haidian fellow member of an association or organization's Fine Chemical Works；Sodium alginate is bought from Qingdao Ding Li seaweed Co., Ltd.
Tablet culture medium provided by the invention includes disintegrating agent.By weight, the disintegrating agent includes 2~8 parts, preferably
It is 4~6 parts, more preferably 5 parts.In the present invention, the disintegrating agent preferably includes dried starch, sodium carboxymethyl starch, low substitution hydroxyl
One or more of third cellulose, crospovidone, croscarmellose sodium and gas-producing disintegrant.In the present invention, institute
The water content for stating disintegrating agent is preferably 8.0%.In the present invention, the disintegrating agent can promote preparation to be disintegrated into junior unit rapidly,
And then it faster dissolves.The present invention is not particularly limited the source of the disintegrating agent, using this field conventional commercial product.
Dried starch, crospovidone are bought from Anhui Shanhe Medicinal Subsidiary Material Co., Ltd. in the embodiment of the present invention；Carboxymethyl starch
Sodium, low-substituted hydroxypropyl cellulose are bought from Distributions in Liaocheng of Shandong Province A Hua Pharmacy stock Co., Ltd；Gas-producing disintegrant is bought from traditional Chinese medicines collection
Chemical reagent Co., Ltd of group.
Tablet culture medium provided by the invention includes lubricant.By weight, the lubricant includes 0.5~2 part, excellent
It is selected as 1~1.5 part, more preferably 1.25 parts.In the present invention, the lubricant preferably includes magnesium stearate, superfine silica gel powder, talcum
One or more of powder, hydrogenated vegetable oil, polyethylene glycols and lauryl sodium sulfate.In the present invention, the lubricant
Water content is preferably 7.5%~8.5%, and more preferably 8%.In the present invention, the lubricant be can reduce between particle, particle and
Frictional force between solid pharmaceutical preparation manufacturing equipment such as tablet punch and the metal contact surface of punch die.The present invention is to the lubricant
Source is not particularly limited, using this field conventional commercial product.Magnesium stearate is bought from Shandong in the embodiment of the present invention
Liaocheng A Hua Pharmacy stock Co., Ltd)；Superfine silica gel powder is bought from Xi'an Yue Lai Pharmaceutical Technology Co., Ltd；Talcum powder purchase is certainly
Fu Da talcum powder Co., Ltd of Laizhou City；Hydrogenated vegetable oil purchase matches hundred million Biotechnology Co., Ltd from Hebei；Polyethylene glycols
It buys from Beijing Haidian fellow member of an association or organization's Fine Chemical Works；Lauryl sodium sulfate is bought from the limited public affairs of Anhui mountains and rivers pharmaceutic adjuvant share
Department.
In the present invention, nutritional ingredient and suitable pH environment etc. needed for dehydrated medium provides microorganism growth are auxiliary
Material adhesive, disintegrating agent and lubricant are played a supporting role in tablet forming process, stability with higher, not with major ingredient
Any physical-chemical reaction occurs, culture medium clarity is not impacted, it is nontoxic to microorganism, have no adverse reaction.
The present invention provides a kind of preparation methods of tablet culture medium described in above scheme, include the following steps:
1) it by adhesive, disintegrating agent, lubricant and accounts for the dehydrated medium of dehydrated medium gross mass 15%~20% and mixes
It closes, obtains premix；
2) premix of the step 1) is mixed, tabletting with surplus dehydrated medium, obtains tablet culture medium.
The present invention is by adhesive, disintegrating agent, lubricant and the dry powder culture for accounting for dehydrated medium gross mass 15%~20%
Base mixing, obtains premix.By mass percentage, the present invention preferably mixes 18% dehydrated medium with auxiliary material.This hair
In bright, the mixed mode is preferably stirred.The time of the stirring is preferably 4~6min, more preferably 5min.It is described to stir
The revolving speed mixed is preferably 5~10r/min, more preferably 8r/min.In the present invention, SBH-600 three-dimensional oscillating is used in embodiment
Mixing machine is stirred.
After obtaining premix, the present invention mixes the premix with surplus dehydrated medium, and tabletting obtains tablet culture
Base.In the present invention, the mixed mode is preferably stirred.The time of the stirring is preferably 4~6min, more preferably
5min.The revolving speed of the stirring is preferably 5~10r/min, more preferably 8r/min.In the present invention, SBH- is used in embodiment
600 three-dimensional oscillating mixers are stirred.The present invention mixes raw material by several times, it is ensured that the uniformity of product.In the present invention,
The pressure when tabletting is preferably 39~41kN, more preferably 40kN.The diameter of obtained tablet is preferably 22mm.
In order to further illustrate the present invention, technical solution provided by the invention is retouched in detail below with reference to embodiment
It states, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
It is in air humidity respectively by pancreas junket soya peptone dehydrated medium, starch, sodium carboxymethylcellulose and magnesium stearate
200 meshes are crossed in 35% room, the water content for adjusting each component respectively is 8%.Weigh 905g pancreas junket soya peptone dry powder
Culture medium, 40g starch, 40g sodium carboxymethylcellulose and 15g magnesium stearate.Tool in the pancreas junket soya peptone dehydrated medium
Body component is as shown in table 1.
1 pancreas junket soya peptone dehydrated medium component list of table
| Components Name | Percentage (%) | The content (g/L) of ingredient in every liter of culture medium |
| Soybean papain hydrolysis object | 10.1 | 3.0 |
| Trypticase | 57.0 | 17.0 |
| Sodium chloride | 16.8 | 5.0 |
| DEXTROSE ANHYDROUS | 7.7 | 2.3 |
| Dipotassium hydrogen phosphate | 8.4 | 2.5 |
By 140g pancreas junket soya peptone dehydrated medium and above-mentioned load weighted starch, sodium carboxymethylcellulose and magnesium stearate
After stirring 4min with the revolving speed of 5r/min, remaining pancreas junket soya peptone dehydrated medium is being added in mixing.Turned again with 10r/min
Speed stirring 6min, is pressed into diameter with the pressure of 39kN as the tablet of 22mm.
Embodiment 2
Weightlessness, pH value, sensitivity and growth rate is dried to the tablet culture medium being prepared in embodiment 1 respectively to examine
It surveys, to determine the quality for the tablet culture medium being prepared.Specific examination criteria is as follows:
The preparation of 1.1 test cultures base fluids
The dissolution of 300mL purified water is added in pancreas junket soya peptone fluid nutrient medium (TSB) piece that 4 embodiments 1 are prepared,
Packing is into container.121 DEG C of high pressure sterilization 15min.
1.2 strain
The passage number of test bacterial strain must not exceed for 5 generations, and (the freeze-drying strain obtained from Culture Collection Center is 0
Generation), the biological characteristics of guarantee test bacterial strain.
The preparation of 1.3 bacterium solutions
Pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis are inoculated in the training of pancreas junket soya peptone liquid respectively
It supports in base or on pancreas junket soya peptone agar medium inclined-plane, 30~35 DEG C are cultivated 18~24 hours.0.9% nothing of above-mentioned culture
The dissolution of bacterium sodium chloride solution, is made 10 compared with normal bacterial opacity tube8Bacteria suspension, then every 1mL is made containing bacterium in 10 times of dilutions
The bacteria suspension of number < 100CFU (Colony Forming Unit).
The fresh cultured object of Candida albicans is inoculated with to Sabouraud dextrose fluid nutrient medium or Sabouraud's dextrose agar culture
On base inclined-plane, 20~25 DEG C are cultivated 24~48 hours, and above-mentioned culture is dissolved with 0.9% aseptic sodium chloride solution, thin with standard
Bacterium opacity tube is relatively made 108Bacteria suspension, then 10 times dilution be made every 1mL containing bacterium number be < 100CFU (Colony Forming Unit)
Bacteria suspension.
On the fresh cultured object of inoculated aspergillus niger to Sabouraud's dextrose agar slant medium inclined-plane.20~25 DEG C of cultures 5
~7 days.0.9% aseptic sodium chloride solution of the 3~5mL containing 0.05% (v/v) polyoxyethylene sorbitan monoleate is added to dissolve, with normal bacterial ratio
Turbid pipe is relatively made 108Bacteria suspension, then it is < 100CFU (Colony Forming Unit) that every 1mL, which is made, containing spore count in 10 times of dilutions
Spore suspension.
1.4 operating procedure
1. final ph
Tablet culture medium after preparing and sterilize according to the above method after placing room temperature, (presses pH using acidometer measurement in accordance with the law
Measuring method), pH value should be 7.3 ± 0.2.
2. loss on drying
This product 1 is taken, is baked to constant weight (weight in 105 DEG C of specific temperature using Mettler HG53 fast tester for water content
Measure difference and be no more than 0.3mg), register instrument correlation values, at least 3 parts of parallel testing seek mean value, and less loss weight must not mistake
6.0%.
3. sensitivity
Sample is prepared according to 1.3 method, test strain is inoculated with direct vaccination ways, takes and trained equipped with pancreas junket soya peptone liquid
11, test tube for supporting base are inoculated with above-mentioned bacterium solution each 2 of < 100CFU respectively, and another 1 is not inoculated with as blank control；Similarly
Test strain is inoculated into control medium by method, synchronous to be counted using the suitable culture medium that counts.Set 30~35 DEG C
In incubator, cultivates 18~48 hours, observe and record result.Blank control pipe answers asepsis growth, be detected culture base tube with it is right
Compare according to culture base tube, test organisms pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis, Candida albicans and black
The equal well-grown of aspergillus, is judged to qualification, is otherwise judged to unqualified.
According to above-mentioned examination criteria detect respectively the tablet culture medium that embodiment 1 is prepared be dried weightlessness, pH value,
Sensitivity and growth rate, specific testing result are as shown in table 2.
The quality measurements of the tablet culture medium of 2 pancreas junket soya peptone fluid nutrient medium of table
Note: the above test stone is based on depending on Chinese Pharmacopoeia 2015 editions.
As can be seen from Table 1, properties after tablet are made in the pancreas junket soya peptone fluid nutrient medium that the present invention is prepared
Meet standards of pharmacopoeia, the results showed that, the tablet culture medium that the present invention is prepared is qualified.
Embodiment 3
Mai Kangkai (MAC) agar dry powder, microcrystalline cellulose, sodium carboxymethylcellulose and magnesium stearate is wet in air respectively
For degree to cross 300 meshes in 35% room, the water content for adjusting each component respectively is 8%.885g MAC is weighed, 60g is micro-
Crystalline cellulose, 50g sodium carboxymethylcellulose and 5g magnesium stearate.Concrete component such as 3 institute of table in the MAC dehydrated medium
Show.
Table 3 Mai Kangkai (MAC) agar dry powder formulation table
177g MAC agar dry powder and above-mentioned load weighted microcrystalline cellulose, carboxymethyl cellulose and magnesium stearate are mixed
It closes, after stirring 5min with the revolving speed of 8r/min, remaining pancreas junket soya peptone liquid dehydrated medium is being added.Turned again with 8r/min
Speed stirring 6min, is pressed into diameter with the pressure of 41kN as Mai Kangkai (MAC) agar tablet of 22mm.
Embodiment 4
The tablet culture medium being prepared using embodiment 3 as experimental group, while using MAC agar dehydrated medium as pair
According to group, weightlessness, pH value, selection performance and growth rate are dried respectively and is detected, to determine the tablet culture being prepared
The quality of base.Specific examination criteria is as follows:
The preparation of 1.1 test cultures base fluids
100mL purified water, dissolution, 121 DEG C of height are added in Mai Kangkai (MAC) the agar tablet that 2 embodiments 3 are prepared
Pressure sterilizing 15min.It is cooled to 50 DEG C or so after sterilizing, topples in 15~20mL to plate, lets cool spare to solidifying.
100mL purified water, dissolution, as control medium is added in 5.15g Mai Kangkai (MAC) agar dry powder.121 DEG C of height
Pressure sterilizing 15min.It is cooled to 50 DEG C or so after sterilizing, topples in 15~20mL to plate, lets cool spare to solidifying.
1.2 strain
The passage number of test bacterial strain must not exceed for 5 generations, and (the freeze-drying strain obtained from Culture Collection Center is 0
Generation), the biological characteristics of guarantee test bacterial strain.
Escherichia coli (Escherichia coli) (ATCC 25922)
Shigella flexneri (Shigellaflexneri) (CMCC (B) 51572)
Staphylococcus aureus (Staphylococcus aureus) (ATCC 6538)
1.3. prepared by bacterium solution
By escherichia coli, shigella flexneri, S. aureus Inoculate to TSB overnight incubation, with 0.9% nothing
Bacterium sodium chloride solution prepares 10 times of bacteria suspensions being serially diluted.
1.4 operating procedure
1. final ph
Tablet culture medium after preparing and sterilize according to the above method after placing room temperature, (presses pH using acidometer measurement in accordance with the law
Measuring method), pH value should be 7.2 ± 0.2, and with compareing, Mai Kangkai (MAC) agar dry powder is consistent.
2. loss on drying
This product 1 is taken, is baked to constant weight in 105 DEG C of specific temperature using Mettler HG53 fast tester for water content, is remembered
Instrument correlation values, at least 3 parts of parallel testing are recorded, mean value is sought, less loss weight must not cross 6.0%, with control Mai Kangkai (MAC)
Agar dry powder is consistent.
3. growth promotion ability+Indication Characteristics
Select escherichia coli, shigella flexneri bacteria suspension 0.1mL (the inoculation level of every plate of appropriate dilution
For 20CFU~200CFU), even spread is inoculated in master plate and control plate TSA respectively.Each dilution is inoculated with two and puts down
Plate.It is placed in constant temperature incubation 20 in 36 ± 1 DEG C of incubators~for 24 hours.1 ring of staphylococcus aureus bacteria suspension is taken with 1 μ L oese,
Media surface to be measured draw six parallel lines, while be inoculated with two plates, be placed in constant temperature incubation 20 in 36 ± 1 DEG C of incubators~
24h。
According to above-mentioned examination criteria respectively to the MAC agar tablet culture medium that embodiment 3 is prepared be dried it is weightless,
PH value, sensitivity and growth rate detection, specific testing result are as shown in table 4.
The quality measurements of table 4MAC agar tablet culture medium
Remarks: the above test stone is based on depending on state food safety verification standard.PR is the rate of recovery in table 4, and G is growth
Index.Calculation method is as follows:
Object bacteria growth rate quantitative measuring method:
The moderate plate of selection clump count is counted, and calculates growth rate by following formula.
In formula:
PR--- growth rate；
NS--- the total plate count obtained on culture medium flat plate to be measured；
N0--- the total plate count obtained on reference culture medium flat plate (total plate count answers >=100CFU).
2 non-targeted bacterium (selectivity) semidefinite weight testing methods
Growth index G is calculated to culture medium by the following method after culture.
Then G is 1 for every scribing line for having denser bacterium colony to grow, up to 6 points on each culture dish.If only half
Line has dense bacterium colony to grow, then G is 0.5.If no bacterium colony growth, increment are raw less than the half or bacterium colony of scribing line in scribing line
Length is faint, then G is 0.The score summation for recording each plate just obtains G.
As can be seen from Table 4, Mai Kangkai (MAC) agar dry powder that the present invention is prepared is made after tablet and dry powder form
Same kind properties it is almost the same, and growth rate is better than the same veriety of common dehydrated medium, meets state food safety
Test stone.
Embodiment 5
By THIOGLYCOLLIC ACID salt broth dry powder, starch, sodium carboxymethyl starch and magnesium stearate respectively in air humidity
To cross 250 meshes in 35% room, the water content for adjusting each component respectively is 8%.Weigh 890g THIOGLYCOLLIC ACID salt stream
Body culture medium dry powder, 50g starch, 50g sodium carboxymethyl starch and 10g magnesium stearate.In the THIOGLYCOLLIC ACID salt dehydrated medium
Concrete component it is as shown in table 5.
5 THIOGLYCOLLIC ACID salt dehydrated medium component list of table
By 160g THIOGLYCOLLIC ACID salt broth dry powder and above-mentioned load weighted starch, sodium carboxymethyl starch and stearic acid
After stirring 5min with the revolving speed of 8r/min, remaining pancreas junket soya peptone liquid dehydrated medium is being added in magnesium mixing.Again with 8r/min
Revolving speed stir 5min, diameter is pressed into as the THIOGLYCOLLIC ACID salt broth tablet of 22mm with the pressure of 40kN.
Embodiment 6
Weightlessness, pH value, spirit are dried to the THIOGLYCOLLIC ACID salt broth tablet being prepared in embodiment 5 respectively
Sensitivity and validity period are detected, to determine the quality for the tablet culture medium being prepared.Specific examination criteria is as follows:
1.1 testing program
Based on the understanding to product property and to the expection of validity period, long term test is chosen 0,3,6,12,18 in storage period,
24,36 months products are investigated.Referring to " Chinese Pharmacopoeia " and ICH (human drugs registration technology requires international coordination meeting) rule
Fixed, long-term stable experiment uses temperature for 25 DEG C ± 2 DEG C, relative humidity 60% ± 10%.
The preparation of test sample: 4 THIOGLYCOLLIC ACID salt broth pieces, which are added the heating of 300mL purified water and are boiled, keeps its complete
Fully dissolved, packing is spare to sterile test tube, and every pipe loading amount is 12mL.121 DEG C of high pressure sterilization 15min.
1.2 verification methods and content
1. physico-chemical examination
Measuring test sample, pH value, each sample are measured 3 times and are averaged as final ph, final pH at room temperature in 25 DEG C
Value should be 7.1 ± 0.2.
2. loss on drying
This product 1 is taken, is baked to constant weight in 105 DEG C of specific temperature using Mettler HG53 fast tester for water content, is remembered
Instrument correlation values, at least 3 parts of parallel testing are recorded, seek mean value, less loss weight must not cross 6.0%.
3. following test
The passage number of test bacterial strain must not exceed for 5 generations, and (the freeze-drying strain obtained from Culture Collection Center is 0
Generation), the biological characteristics of guarantee test bacterial strain.
Staphylococcus aureus, pseudomonas aeruginosa, escherichia coli are inoculated in pancreas junket soya peptone Liquid Culture respectively
In base, 30~35 DEG C are cultivated 18~24 hours.Clostridium sporogenes is inoculated in THIOGLYCOLLIC ACID salt broth, 30~35 DEG C of trainings
Support 18~24 hours, above-mentioned culture with 0.9% aseptic sodium chloride solution be made every 1mL containing bacterium number for no more than bacteria suspension.
It is inoculated with above-mentioned bacterium solution respectively to tested culture medium, each test strain 3 is managed, and another 1 is not inoculated with as blank control,
It sets 30~35 DEG C to cultivate 24~72 hours, observes and records result.
Result judgement: blank control pipe answers asepsis growth, test organisms pseudomonas aeruginosa, staphylococcus aureus, large intestine
Angstrom uncommon bacterium and the equal well-grown of clostridium sporogenes, are judged to qualification, are otherwise judged to unqualified.The above test stone is based on Chinese Pharmacopoeia
Depending on 2015 editions.
The THIOGLYCOLLIC ACID salt fluid tablet culture medium that embodiment 5 is prepared is done respectively according to above-mentioned examination criteria
Dry weightlessness, pH value, sensitivity and growth rate detection, specific testing result is as shown in tables 6 and 7.
PH value, the loss on drying of 6 THIOGLYCOLLIC ACID salt fluid tablet culture medium of table
| Holding time | PH value | Loss on drying value |
| 0 month | 7.11 | 2.46% |
| 3 months | 7.12 | 2.46% |
| 6 months | 7.11 | 2.45% |
| 12 months | 7.13 | 2.44% |
| 18 months | 7.12 | 2.44% |
| 24 months | 7.11 | 2.44% |
| 36 months | 7.12 | 2.43% |
The sensitivity test result of 7 THIOGLYCOLLIC ACID salt fluid tablet culture medium of table
It is 25 DEG C ± 2 DEG C, stores this product validity period under relative humidity 60% ± 10% in temperature it can be seen from table 6 and 7
It can achieve 3 years, and indices reach Chinese Pharmacopoeia 2015 editions requirements.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
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| CN114555776A (en) * | 2019-09-19 | 2022-05-27 | 生命技术公司 | Cell culture medium tablet and manufacturing method |
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