CN108812698A - A kind of bactericidal composition and its application containing jinggangmeisu - Google Patents
A kind of bactericidal composition and its application containing jinggangmeisu Download PDFInfo
- Publication number
- CN108812698A CN108812698A CN201810488707.XA CN201810488707A CN108812698A CN 108812698 A CN108812698 A CN 108812698A CN 201810488707 A CN201810488707 A CN 201810488707A CN 108812698 A CN108812698 A CN 108812698A
- Authority
- CN
- China
- Prior art keywords
- jinggangmeisu
- bei laisi
- bactericidal composition
- laisi bacillus
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 17
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 9
- 239000000470 constituent Substances 0.000 claims abstract description 4
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 19
- 241000221785 Erysiphales Species 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 6
- 238000013329 compounding Methods 0.000 claims description 5
- 241000726221 Gemma Species 0.000 claims description 4
- 244000299906 Cucumis sativus var. sativus Species 0.000 claims 1
- 230000000857 drug effect Effects 0.000 abstract description 6
- 239000000575 pesticide Substances 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000002045 lasting effect Effects 0.000 abstract description 4
- 229940079593 drug Drugs 0.000 abstract description 2
- 230000010534 mechanism of action Effects 0.000 abstract description 2
- 240000008067 Cucumis sativus Species 0.000 description 18
- 238000000034 method Methods 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000009036 growth inhibition Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 231100000820 toxicity test Toxicity 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 208000025309 Hair disease Diseases 0.000 description 1
- 231100000605 Toxicity Class Toxicity 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000034653 disorder of pilosebaceous unit Diseases 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of bactericidal composition and its application containing jinggangmeisu, belongs to cultural control technical field.The bactericidal composition contains active constituent jinggangmeisu and Bei Laisi bacillus, is set as 100% female medicine with the Bei Laisi bacillus containing 100,000,000,000 cfu/g, the weight ratio of the jinggangmeisu and Bei Laisi bacillus is 15:1~1:15.The present invention have the following advantages that with significant technical effect, the mechanism of action of jinggangmeisu and Bei Laisi bacillus is different, and the two mixture is synergistic obvious, can increase substantially drug effect, reduces Pesticide use amount and drug cost.Meanwhile composition of the invention can improve the lasting period of jinggangmeisu.
Description
Technical field
The invention belongs to cultural control technical fields, and in particular to a kind of bactericidal composition containing jinggangmeisu and its answer
With.
Background technique
Powdery mildew of cucumber is commonly called as " white hair disease ", is one of Common Diseases in cucumber cultivation, because powdery mildew influences the light of blade
Cooperation is used, and very big production loss is caused to the cucumber growth later period.Certain drug resistance is especially generated in recent years, makes the general time
10% or so, the popular time underproduction prevents and treats powdery mildew of cucumber at present and generallys use chemical agent, exist up to 40% or so for the underproduction
The risk of pesticide residue, need the biological pesticide for developing a kind of high-efficiency low-toxicity.
Jinggangmeisu is also known as valida, is a kind of antibiotic that actinomyces generate, has stronger absorbability, easily by bacterium
Body cell is absorbed and is conducted rapidly in it, interferes and inhibit somatic cells growth and development.Belong to less toxic fungicide.Sterling size
Mouse acute oral LD50 is all larger than 2000 mgs/kg, and subcutaneous injection LD50 is all larger than 1500 mgs/kg.5000 milligrams/public
Jin smears rat skin without toxic reaction.To fish low toxicity, carp TLM (96h) LD50>40 mg/litres.Jinggangmeisu cucumber
Powdery mildew preventive effect is preferable, but since it is single dose fungicide, there are resistance risks.
Bei Laisi bacillus is the biocontrol bacteria obtained from ocean.Bei Laisi bacillus can not only effectively inhibit
The growth of pathogen, and stimulation plant growth can be generated and induce the compound of plant defense response, soil root can be improved
The microenvironment of system enhances the growing way and disease resistance of plant, improves crop yield and income.
Jinggangmeisu and Bei Laisi bacillus have the advantages that small toxicity as biological pesticide, but its disadvantage is also very bright
Aobvious, the jinggangmeisu prevention and treatment powdery mildew of cucumber lasting period is short, and Bei Laisi bacillus prevention and treatment powdery mildew of cucumber drug effect is poor, therefore, prevents
The share for controlling chemical pesticide in the medicament of powdery mildew of cucumber is increasing, but chemical pesticide toxicity is bigger than biological pesticide, influences to eat
Therefore product safety needs to develop the biological bactericide that a kind of effect is good, and toxicity is low, and the lasting period is grown.
Summary of the invention
In view of the problems of the existing technology, it is an object of the invention to design to provide a kind of sterilization group containing jinggangmeisu
Close the technical solution of object and its application.
A kind of bactericidal composition containing jinggangmeisu, it is characterised in that contain active constituent jinggangmeisu and shellfish Lay
This bacillus is set as 100% female medicine, the jinggangmeisu and shellfish Lay with the Bei Laisi bacillus containing 100,000,000,000 cfu/g
The weight ratio of this bacillus is 15:1~1:15.
A kind of bactericidal composition containing jinggangmeisu, it is characterised in that the jinggangmeisu and Bei Laisi gemma
The weight ratio of bacillus is 5:1~1:8.
A kind of bactericidal composition containing jinggangmeisu, it is characterised in that the jinggangmeisu and Bei Laisi gemma
The weight ratio of bacillus is 1:1.
A kind of bactericidal composition containing jinggangmeisu, it is characterised in that the jinggangmeisu is in compounding agent
Content is 4-30%, and content of the Bei Laisi bacillus in compounding agent is 500,000,000 cfu/g-20, hundred million cfu/g.
A kind of application of the bactericidal composition containing jinggangmeisu in prevention and treatment powdery mildew of cucumber.
The bactericidal composition containing jinggangmeisu is added corresponding according to method known in those skilled in the art
The dosage forms such as aqueous suspension agent, wettable powder are made in adjuvant.
The adjuvant includes filler, dispersing agent, wetting agent, stabilizer or other is beneficial to effective component in the formulation
The substance that stable and drug effect plays, be all commonly used in pesticidal preparations or allow using various composition, specific ingredient and dosage
It is determined according to recipe requirements by simple experiment.
The present invention has the following advantages that and significant technical effect, the mechanism of action of jinggangmeisu and Bei Laisi bacillus
Difference, the two mixture is synergistic obvious, can increase substantially drug effect, reduces Pesticide use amount and drug cost.Meanwhile this hair
Bright composition can improve the lasting period of jinggangmeisu.
Specific embodiment
Further illustrate the present invention with reference to embodiments.Percentage of the present invention is weight percentage.This
The embodiment of invention is the method combined using indoor toxicity test and field trial.
Indoor toxicity test of 1 composite jinggangmycin of embodiment to powdery mildew of cucumber
Cucumber powdery mildew bacteria is acquired by field in the present embodiment, is cultivated through indoor purifying.
Test method：After prerun determines the effective inhibition concentration range of each medicament, by Bei Laisi bacillus, jinggangmeisu
And its mixture preparation dilutes 5 concentration by active constituent content gradient series, is compared with sterile water, using mycelial growth rate
Method measurement, various concentration medicament is added in sterilizes culture dish, every ware 1mL, is added the PDA culture medium 9mL that sterilizing is melted, is filled
Divide to mix and plate is made, then the bacterium colony to spread cultivation is reached to the bacteria cake of 0.5cm, every ware is a piece of, and 3 repetitions of every processing are placed in 28 DEG C
It is cultivated in insulating box, cultivates 4 days measurement colony diameters, calculate mycelial growth inhibition rate, find out virulence regression equation, calculate EC50, bacterium
Silk growth inhibition ratio formula is as follows：
Mycelial growth inhibition rate（%）=（Blank control colony diameter-chemicals treatment colony diameter）/ blank control colony diameter *
100。
With the co-toxicity coefficient method of Sun Yunpei（CTC）Evaluate the synergistic effect of pharmacy mix, i.e., CTC≤80 are that antagonism is made
With 80<CTC<120 be summation action, and CTC≤120 are synergistic effect.
It is set as 100% female medicine in the embodiment with the Bei Laisi bacillus containing 100,000,000,000 cfu/g to be compounded.
1 jinggangmeisu of table compounds the indoor toxicity test table to powdery mildew of cucumber with Bei Laisi bacillus
As can be seen from Table 1, jinggangmeisu, EC of the Bei Laisi bacillus to powdery mildew of cucumber50Respectively 18.68mg/L and
39.57mg/L, when jinggangmeisu and Bei Laisi bacillus mass ratio are 15:1~1:When 15, co-toxicity coefficient is greater than 120, performance
Synergistic effect out, when jinggangmeisu and Bei Laisi bacillus mass ratio are 1:When 1, co-toxicity coefficient is maximum, and synergistic effect is best.
Embodiment two is by jinggangmeisu and Bei Laisi bacillus by weight 1:2 compoundings, carry out the field of powdery mildew of cucumber prevention and treatment
Between test, different agents are made into respective concentration by the requirement of according to the form below, are administered for the first time in cucumber period, second after 7 days
Application, twice, 7 days after application, 14 days, 21 days measurement disease indexs calculated drug effect for application altogether.
Table 2 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
（Data source was in field control effectiveness test in 2017）.
Embodiment three is by jinggangmeisu and Bei Laisi bacillus by weight 3:1 compounding, carries out powdery mildew of cucumber prevention and treatment
Different agents are made into respective concentration by field trial, the requirement of according to the form below, are administered for the first time in cucumber period, second after 7 days
Secondary application, twice, 7 days after application, 14 days, 21 days measurement disease indexs calculated drug effect for application altogether.
Table 3 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
（Data source was in field control effectiveness test in 2017）.
Claims (5)
1. a kind of bactericidal composition containing jinggangmeisu, it is characterised in that contain active constituent jinggangmeisu and Bei Laisi gemma bar
Bacterium is set as 100% female medicine, the jinggangmeisu and Bei Laisi gemma bar with the Bei Laisi bacillus containing 100,000,000,000 cfu/g
The weight ratio of bacterium is 15:1~1:15.
2. a kind of bactericidal composition containing jinggangmeisu according to claim 1, it is characterised in that the jinggangmeisu
Weight ratio with Bei Laisi bacillus is 5:1~1:8.
3. a kind of bactericidal composition containing jinggangmeisu according to claim 1, it is characterised in that the jinggangmeisu
Weight ratio with Bei Laisi bacillus is 1:1.
4. a kind of bactericidal composition containing jinggangmeisu according to claim 1, it is characterised in that the jinggangmeisu
Content in compounding agent is 4-30%.
5. a kind of bactericidal composition containing jinggangmeisu according to claim 1 to 4 is in prevention and treatment powdery mildew of cucumber
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810488707.XA CN108812698B (en) | 2018-05-21 | 2018-05-21 | Validamycin-containing bactericidal composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810488707.XA CN108812698B (en) | 2018-05-21 | 2018-05-21 | Validamycin-containing bactericidal composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108812698A true CN108812698A (en) | 2018-11-16 |
| CN108812698B CN108812698B (en) | 2020-11-17 |
Family
ID=64148980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810488707.XA Active CN108812698B (en) | 2018-05-21 | 2018-05-21 | Validamycin-containing bactericidal composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108812698B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383797A (en) * | 2021-05-28 | 2021-09-14 | 浙江省桐庐汇丰生物科技有限公司 | Tetramycin-containing bactericidal composition |
| CN119817583A (en) * | 2025-03-18 | 2025-04-15 | 浙江省桐庐汇丰生物科技有限公司 | Bactericide containing validamycin and chinaberry terpene ketolide and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964514A (en) * | 2017-09-08 | 2018-04-27 | 华夏春秋科技发展（北京）有限责任公司 | A kind of Bei Laisi bacillus and its application on plant |
-
2018
- 2018-05-21 CN CN201810488707.XA patent/CN108812698B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964514A (en) * | 2017-09-08 | 2018-04-27 | 华夏春秋科技发展（北京）有限责任公司 | A kind of Bei Laisi bacillus and its application on plant |
Non-Patent Citations (1)
| Title |
|---|
| 徐利剑: ""井冈霉素的研究进展"", 《中国农学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383797A (en) * | 2021-05-28 | 2021-09-14 | 浙江省桐庐汇丰生物科技有限公司 | Tetramycin-containing bactericidal composition |
| CN113383797B (en) * | 2021-05-28 | 2022-07-29 | 浙江省桐庐汇丰生物科技有限公司 | Tetramycin-containing bactericidal composition |
| CN119817583A (en) * | 2025-03-18 | 2025-04-15 | 浙江省桐庐汇丰生物科技有限公司 | Bactericide containing validamycin and chinaberry terpene ketolide and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108812698B (en) | 2020-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105145640B (en) | A kind of bactericidal composition containing trichoderma harzianum | |
| CN106234388B (en) | A kind of composition pesticide of alkene containing benzo fluorine bacterium azoles and jamaicin | |
| CN108812698A (en) | A kind of bactericidal composition and its application containing jinggangmeisu | |
| CN104705326B (en) | A kind of bactericidal composition containing Zhongshengmycin and tiger glue adipic acid copper | |
| CN106342832A (en) | Fluoxastrobin and physcion containing sterilization composition | |
| CN106035334B (en) | A kind of composition pesticide containing amino-oligosaccharide and fludioxonil | |
| CN112772658A (en) | Bactericidal composition of spirosporium roseum spores and boscalid and application of bactericidal composition | |
| CN114027300B (en) | Application of isoeugenol and its methylated metabolites in inhibiting Phytophthora infestans and controlling plant diseases | |
| CN110402929A (en) | A kind of bactericidal composition and its application containing jinggangmeisu | |
| CN101617674A (en) | Bacteria and pest killing compound agent for wheat | |
| CN111109295B (en) | Pesticide composition with kasugamycin and nano-silver as active components and application thereof | |
| CN107318865A (en) | The composition pesticide of captan and furametpyr compounding | |
| CN106561677A (en) | Pesticide composition containing 2-methyl sufone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole and amino-oligosaccharin and application of pesticide composition | |
| CN105165899A (en) | Sterilization composition containing trichoderma harzianum and metalaxyl | |
| CN106342833B (en) | A kind of bactericidal composition containing fluoxastrobin and Osthole | |
| CN117918368B (en) | Use of a fungicide composition containing benzimidazole and pyraclostrobin for the control of anthracnose diseases in plants. | |
| CN118743396B (en) | A bactericidal composition containing benzovinflumizone and fosetyl aluminum and its application | |
| CN109699664A (en) | A kind of seed coat agent containing captan and its application | |
| CN113383797B (en) | Tetramycin-containing bactericidal composition | |
| CN114680121A (en) | Bactericidal composition containing hemin and S-abscisic acid | |
| CN108812700A (en) | A kind of granule containing Zhongshengmycin and bacillus subtilis | |
| CN119014413A (en) | Insecticidal and fungicidal composition and its application | |
| CN108041077A (en) | A kind of soil fungicides | |
| CN106962367A (en) | A kind of composition pesticide containing zinc thiazole and its application | |
| CN105994303B (en) | A kind of bactericidal composition containing fluoxastrobin and chitosan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |