CN108669566A

CN108669566A - A kind of functional composition and its application

Info

Publication number: CN108669566A
Application number: CN201810205335.5A
Authority: CN
Inventors: 王羚郦
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-03-13
Filing date: 2018-03-13
Publication date: 2018-10-19

Abstract

Embodiment of the disclosure provides a kind of functional composition, including basic ingredient, and the basic ingredient includes salinity, vitamin, sugar；Functional component, including the first functional component and/or the second functional component, first functional component include Sessileflower Acanthopanax Bark, sealwort, matrimony vine；The second composition includes soya-bean polypeptides, olive polyphenol.The functional composition that the embodiment of the present disclosure provides as functional health product, functional medicines, functional food etc. at least partly it is antifatigue, regain one's strength, improve constitution and other effects.

Description

A kind of functional composition and its application

Technical field

The embodiment of the present disclosure is related to a kind of functional composition and its application, belongs to medical health field.

Background technology

The intensified competition of modern society, tempo increase are chronically at nervous living environment, and operating pressure is big and excessively tired Labor so that part population is chronically at the sub-health state of fatigue, over fatigue, constitution difference, brings long-term pathogenic risk. Therefore, how delay fatigue, dispelling fatigue, regaining one's vigor, improve constitution etc. has urgent demand.

For the demand, the health products form such as drinks, functional food has been greatly developed in recent years, market On there is various different forms, this kind of product of different levels, to meet different types of health care demand.Common such production Product include：1, red ox, for supplementing physical efficiency；2, Jia get Le, it is core to supplement water and electrolyte, to meet the physical efficiency of sport people Expending equilibrium；3, it pulses, for supplementing lot of trace vitamin, improves nutrition；4, lung health is protected in true field, is improved specific Organ function.

Applicant have observed that many health foods, the drink etc. sold on the market at present, past in order to realize its function Toward using a variety of parahormone chemical compositions, such as taurine, lysine, caffeine, vitamin etc., these chemical additives are long-term There may be potential safety, additive risks for intake.

Invention content

Embodiment of the disclosure provides a kind of functional composition and its application, is at least partially and regains one's strength, Dispelling fatigue nourishes body, improves the function of constitution.

In one aspect of the present disclosure, a kind of functional composition, including basic ingredient, the basic ingredient packet are provided Include salinity, vitamin, sugar；Functional component, including the first functional component and/or the second functional component, first function at It includes Sessileflower Acanthopanax Bark, sealwort, matrimony vine to divide；The second composition includes soya-bean polypeptides, olive polyphenol.

Optionally, in the basic ingredient, the dosage number of each component is 0.5-3 parts of salinity, vitamin 0.005- 0.03 part, 5-10 parts of sugar.

Optionally, in first functional component, the dosage number of each component is 2-5 parts of Sessileflower Acanthopanax Bark, sealwort 8-15 Part, 8-15 parts of matrimony vine.

Optionally, in second functional component, the dosage number of each component is 1-3 parts of soya-bean polypeptides, olive polyphenol 0.05-0.3 parts.

Optionally, the functional composition, including basic ingredient, the first functional component and the second functional component, respectively The dosage number of component be 1 part of salinity, 0.015 part of vitamin, 8 parts of sugar, 4 parts of Sessileflower Acanthopanax Bark, 10 parts of sealwort, 10 parts of matrimony vine, 2 parts of soya-bean polypeptides, 0.1 part of olive polyphenol.

Optionally, the salinity includes sea salt, and the vitamin includes vitamin B2 and/or vitamin B6, the sugar Including glucose.

In another aspect of the present disclosure, provides above-mentioned functional composition and preparing antifatigue medicine as active constituent Application in product, health products or food.

In the further aspect of the disclosure, above-mentioned functional composition is provided as active constituent in the medicine for preparing resist oxygen lack Application in product, health products or food.

Optionally, the drug, health products or food are Oral liquid forms, the mass-volume concentration of functional composition For 0.1-0.2g/ml.

Optionally, the drug, health products or food are Oral liquid forms, dosage 1-100ml/kg.

The experiment that applicant carries out shows that the functional composition of the embodiment of the present disclosure has the preferable oxygen of resistance to institute and resists tired Work is used, and showing can regain one's strength faster in short term, improves fatigue, and long-time service can nourish, improve constitution.

Specific implementation mode

To keep the purpose, technical scheme and advantage of the embodiment of the present disclosure clearer, below in conjunction with the embodiment of the present disclosure Composition, experiment, the technical solution of the embodiment of the present disclosure is clearly and completely described.Obviously, described embodiment is A part of this disclosure embodiment, instead of all the embodiments.Based on described embodiment of the disclosure, this field is common The every other embodiment that technical staff is obtained under the premise of without creative work belongs to the model of disclosure protection It encloses.

The functional composition that each Ingredient Amount mentioned in following embodiments does not provide the disclosure constitutes special It limits, also not limiting the embodiment of the present disclosure must be realized with mentioned dosage, be only schematically illustrate present disclosure.

In some embodiments, present disclose provides a kind of functional composition, including basic ingredient, the basic ingredients Including salinity, vitamin, sugar；Functional component, including the first functional component and/or the second functional component, first function Ingredient includes Sessileflower Acanthopanax Bark, sealwort, matrimony vine；The second composition includes soya-bean polypeptides, olive polyphenol.

For example, salinity also refers to as biologically edible salt, including but not limited to well salt, rock salt, sea salt, Black salt etc..

For example, salinity also refers to sea salt.

For example, salinity is also referred to by drying the salinity in the seawater that seawater obtains.

For example, salinity also refers to the sea salt with following mass ratio composition：Chlorion 55.03%, sodium ion 30.59%, sulfate radical, 7.68%, magnesium ion 3.68%, calcium ion 1.18%, potassium ion, 1.11%, bicarbonate radical 0.41%, Bromide ion 0.19%, borate 0.08%, strontium ion 0.04%, other (impurity) 0.01%.

For example, vitamin also refers to the vitamin ingredients that multiple beneficial is needed in function of human body, including but not limited to Vitamin B complex, D, A, E etc..

For example, vitamin may include vitamin B2 and/or vitamin B6.

For example, sugar also refer to it is various for absorption of human body, decompose to be converted to the carbohydrate of energy, packet Include but be not limited to glucose, maltose, lactose etc..

For example, sugar may include glucose.

For example, in the first functional component, its crude drug may be used in Sessileflower Acanthopanax Bark, sealwort, matrimony vine, and can also use will Medicinal material crush after medicinal powder, its extract can also be used.For example, water-soluble or fat-soluble extract；For example, alcohol or/ And water extract.

For example, in the second functional component, soya-bean polypeptides, olive polyphenol can directly use such active ingredient, Extract of soybean, the olive extract for including these active ingredients may be used, the powder after soybean, olive can also be used to crush End.

For example, in basic ingredient, the dosage number of each component is 0.5-3 parts of salinity, 0.005-0.03 parts of vitamin, sugar Divide 5-10 parts.

For example, vitamin includes vitamin B2, vitamin B6, the dosage portion rate of the two is (3-7)：(7-15).

For example, in basic ingredient, the dosage number of each component is 1 part of sea salt, 0.005 part of vitamin B2, vitamin B60.01 parts, 8 parts of glucose.

For example, in the first functional component, the dosage number of each component is 2-5 parts of Sessileflower Acanthopanax Bark, 8-15 parts of sealwort, matrimony vine 8-15 parts.

For example, with crude drug gauge, in the first functional component, the dosage number of each component is 2-5 parts of Sessileflower Acanthopanax Bark, sealwort 8-15 parts, 8-15 parts of matrimony vine.

For example, with crude drug gauge, in the first functional component, the dosage number of each component is 4 parts of Sessileflower Acanthopanax Bark, sealwort 10 Part, 10 parts of matrimony vine.

For example, in the second functional component, the dosage number of each component is 1-3 parts of soya-bean polypeptides, olive polyphenol 0.05- 0.3 part.

For example, in terms of compound amount, in the second functional component, the dosage number of each component be 1-3 parts of soya-bean polypeptides, 0.05-0.3 parts of olive polyphenol.

For example, in terms of compound amount, in the second functional component, the dosage number of each component is 2 parts of soya-bean polypeptides, olive 0.1 part of olive polyphenol.

For example, functional composition, including basic ingredient, the first functional component and the second functional component, the use of each component Amount number is 1 part of salinity, 0.015 part of vitamin, 8 parts of sugar, 4 parts of Sessileflower Acanthopanax Bark, 10 parts of sealwort, 10 parts of matrimony vine, soya-bean polypeptides 2 Part, 0.1 part of olive polyphenol.

For example, functional composition, including basic ingredient, the first functional component and the second functional component, the use of each component Amount number is 1 part of sea salt, 0.005 part of vitamin B2,0.01 part of vitamin B6,8 parts of glucose, Sessileflower Acanthopanax Bark (with crude drug amount Meter) 4 parts, 10 parts of sealwort (with crude drug gauge), 10 parts of matrimony vine (with crude drug gauge), 2 parts of soya-bean polypeptides (with compound gauge), olive 0.1 part of olive polyphenol (with compound gauge).

Embodiment of the disclosure additionally provides the preparation process of above-mentioned functional composition, can be various by the field of Chinese medicines It is prepared by common technology.

For example, it may be Sessileflower Acanthopanax Bark, sealwort, matrimony vine, soybean, olive etc. are crushed respectively, by powder and salinity, Sugar, vitamin etc. are mixed.

For example, can be carried to crude drug powders such as Sessileflower Acanthopanax Bark, sealwort, matrimony vine, soybean, olives by water, ethyl alcohol etc. Extract is obtained, then mixes it with salinity, sugar, vitamin etc..

For example, it may be Sessileflower Acanthopanax Bark, sealwort, matrimony vine etc. are crushed as crude drug, it is more with soya-bean polypeptides, olive Phenol, salinity, sugar, vitamin etc. are mixed.

Embodiment of the disclosure additionally provide above-mentioned functional composition as active constituent prepare antifatigue drug, Application in health products or food.

Embodiment of the disclosure additionally provide above-mentioned functional composition as active constituent the drug for preparing resist oxygen lack, Application in health products or food.

For example, the concrete form of drug, health products or food can be functional composition is used in combination with auxiliary material with Obtain corresponding dosage form or product form.

For example, auxiliary material may include any nutrition, the biologies such as excipient, antioxidant, PH conditioning agents, preservative, isotonic agent Acceptable on, the guaranteed auxiliary material of safety.

For example, the concrete form of drug, health products or food can be capsule, tablet, pulvis, granule, oral solution or Syrup etc., the appearance existence form for changing product belongs to the technical solution that those skilled in the art can associate, therefore changes The shape of final products belongs to the coverage area of the disclosure.

For example, the drug, health products or food are Oral liquid forms, by mixing functional composition in water It uniformly obtains, the mass-volume concentration of functional composition is 0.1-0.2g/ml.

For example, the drug, health products or food are Oral liquid forms, dosage 1-100ml/kg.Referred to herein Dosage refers to the weight relative to organism weight, such as people, in terms of daily, the oral solution dosage volume drunk.

For example, the drug, health products or food are Oral liquid forms, dosage 2-50ml/kg.

For example, the drug, health products or food are Oral liquid forms, dosage 8-40ml/kg.

In order to illustrate the effect of disclosure functional composition, applicant carried out following experiments.

1 materials and methods

1.1 animal subject

SPF grades of kunming mices, male, 20 ± 2g of weight, are provided by Guangdong Province's Experimental Animal Center.Mouse is 18 in temperature ~25 DEG C, relative humidity be 50%~70% environment in raise.

1.2 basal feed

It is provided by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center.

1.3 test sample

Blank control group：Distilled water；

Positive controls：Commercially available red ox drinks；

1 group of the side of tearing open：Sessileflower Acanthopanax Bark 4g (crude drug amount), sealwort 10g (crude drug amount), matrimony vine 10g (crude drug amount), glucose 8g, Sea salt 1g, vitamin B2 5mg, vitamin B6 10mg；

2 groups of the side of tearing open：Soya-bean polypeptides 2g, olive polyphenol 100mg, glucose 8g, sea salt 1g, vitamin B2 5mg, vitamin B6 10mg。

Test group：Sessileflower Acanthopanax Bark 4g (crude drug amount), sealwort 10g (crude drug amount), matrimony vine 10g (crude drug amount), soya-bean polypeptides 2g, Olive polyphenol 100mg, glucose 8g, sea salt 1g, vitamin B2 5mg, vitamin B6 10mg.

The above-mentioned side's of tearing open combination and test group are prepared as 250ml oral solutions.

1.4 reagents and instrument

Blood lactase acid assay kit, serum urea nitrogen assay kit are built up institute of biological products by Nanjing and are provided.

722 type spectrophotometers, XHF-1 types high speed disperser are provided by Traditional Chinese Medicine University Of Guangzhou experimental center.

1.5 experimental method

It presses《Function of health food assessment process and the method for inspection》It carries out.

1.5.1 experimental animal is grouped

Dosage grouping is equivalent to 2ml/kg according to daily life function beverage human intaking amount 120ml/d by 60kg batheroom scales, Folding and the equivalent dose conversion coefficient of mouse are 9.1, are equivalent to 18.2ml/kg, and test group is designed by 0.5,1,2 times of equivalent 1,2 group of basic, normal, high three dosage group, " red ox " positive controls and the side of tearing open are designed by 1 times of equivalent, as follows：

(1) blank control group：18.2ml/kg

(2) positive controls：18.26ml/kg

(3) 1 group of the side of tearing open：18.2ml/kg

(4) 2 groups of the side of tearing open：18.2ml/kg

(5) test group low dosage：9.1ml/kg

(6) test group middle dosage：18.2ml/kg

(7) test group high dose：36.4ml/kg

1.5.2 hypoxia endurance test method

Tested material 30d continuously is given, each group mouse is respectively put into and fills 15g sodium stones by the 1h after last gives tested material In the 250mL wide-mouth bottle of ash (every bottle only puts 1 mouse), bottleneck is smeared with vaseline, covers tightly, is allowed to air tight, immediately timing, Stopped as index with breathing, the observation mouse time dead due to anoxic.

1.5.3 swimming with a load attached to the body is tested

Continuous to give tested material 30d, after test last is to sample 30min, the sheet lead of mouse 5% weight of heavy burden is placed in water temperature 25 DEG C, the water tank went swimming of 35~40cm of the depth of water, record mouse from swimming to the time of death, when swimming as mouse Between (min).

1.5.4 the measurement of blood lactase acid

1.5.4.1 blood lactase acid measures before swimming

It is grouped and to sample:Same 1.5.2, last give sample 30min posterior orbits blood sampling, take serum with 722 type spectrophotometers into Row Plasma lactate.

1.5.4.2 0min blood lactase acids measure after swimming

It is grouped and to the same 1.5.2 of sample.Last is adopted to the water went swimming 90min in 30 DEG C of temperature after sample 30min, immediately eye socket Blood takes serum to carry out Plasma lactate with 722 spectrophotometers.

1.5.4.3 30min blood lactase acids measure after swimming

It is grouped and to the same 1.5.2 of sample.Last is to water the went swimming 90min, rest 30min in 30 DEG C of temperature after sample 30min Eye socket is taken a blood sample immediately afterwards, and serum is taken to carry out Plasma lactate with 722 spectrophotometers.

1.5.4.4 the measurement of serum urea nitrogen

It is grouped and to sample:Same 1.5.2.Last to after sample 30min after the water went swimming 90min of 30 DEG C of temperature, eye immediately Socket of the eye is taken a blood sample, and serum is taken to carry out determination of urea nitrogen with 722 spectrophotometers.

1.5.4.5 the measurement of hepatic glycogen

It is grouped and to sample:Same 1.5.2.Last is put to death immediately to the water went swimming 90min in 30 DEG C of temperature after sample 30min, Liver is taken to be blotted with filter paper after physiological saline rinses.Liver 100mg is weighed, 8mL TCA are added, 1min is homogenized, by homogenate Centrifuge tube is poured into, 15min is centrifuged with 3000r/min, supernatant 1mL is taken, adds 95% ethyl alcohol 4mL, mix well to two kinds of liquid Between there are interfaces, beyond the Great Wall with clean plug, at room temperature erect stand overnight.Next day centrifuges 15min with 3000r/min.Carefully It outwells supernatant and test tube is made to stand upside down and place 10min.Glycogen is dissolved with 2mL distilled water.Reagent blank pipe:2mL distilled water is inhaled to arrive Clean centrifuge tube standard pipe:It inhales 0.5mL glucose standards (100mg/dL) and 1.5mL distilled water is put into same pipe.It will Each pipe is added in 10mL anthrone reagents, boils 15min, then moves on to ice-water bath and uses reagent blank after cooling under 620nm wavelength Absorbance is measured after pipe zeroing.Hepatic glycogen content is calculated as follows:

Hepatic glycogen (mg/100g livers)=(DU/DS) × 0.5 × (V/G) × 100 × 0.9

In formula：DU --- sample cell absorbance；V --- extracting liquid volume (mL)；DS --- standard pipe absorbance； 0.5 --- the glucose content in 0.5mL glucose standards；G --- hepatic tissue weight (g)；0.9 --- glucose is converted At the coefficient of glycogen.

1.6 data processing

Data statistic analysis is carried out with SPSS10.0 for windows.

2 results and analysis

Influence of 2.1 drinks to mouse weight

Influence (X ± SD) of 1 drinks of table to mouse weight

As shown in Table 1, after mouse einnehmen function beverage 30d, mouse weight is compared with the control group without significant difference (p> 0.05).Each group will not cause weight excessively rapid growth, will not inhibit the normal development of mouse.

Influence of 2.2 drinks to mouse hypoxia-bearing capability

Influence (X ± SD) of 2 drinks of table to mouse hypoxia-bearing capability

*：With blank control group ratio, P<0.05；#：Ratio, P are organized with red ox control group, the side of tearing open<0.05

**：With blank control group ratio, P<0.01；##：Ratio, P are organized with red ox control group, the side of tearing open<0.01

Anoxic is a kind of pessimal stimulation to body, influences the various metabolism of body, as can be seen from Table 2,6 groups of drinks and blank Control group can more significantly improve mouse hypoxia-bearing capability (P<0.01).Meanwhile each dosage group of test group and red ox control group, The side's of tearing open control group ratio, significant difference, prompt test group group to be substantially better than in terms of hypoxia-bearing capability red ox control group and The side's of tearing open control group.

Influence of 2.3 drinks to the mice burden swimming time

Influence (X ± SD) of 3 drinks of table to the mice burden swimming time

As shown in Table 3, after gavaging drinks 30d, there is significant extension (p in each group mice burden swimming time<0.05). For wherein high, middle dose group compared with red ox and the side's of tearing open group control group, the extension time is longer, there is notable difference (P<0.05) it, shows Influence of the test group to the mice burden swimming time will be substantially better than red ox and the side's of tearing open control group.

Influence of 2.4 drinks to mouse movement biochemical indicator

Influence (X ± SD) of 4 drinks of table to mouse blood lactase acid

As shown in table 4, the measurement of blood lactase acid, after swimming after 0min and 30min, other than 1 group of the side of tearing open, remaining each group function Beverage mouse blood lactase acid is significantly lower than blank control group (p ＜ 0.01), the high, medium and low dosage group of test group and red ox control group Compare no significant difference between 2 groups of the side of tearing open.

Influence (X ± SD) of 5 drinks of table to mice serum urea nitrogen, hepatic glycogen

Shown in table 5, the urea nitrogen levels of 1 group of the middle and high dosage of test group and the side of tearing open are significantly lower than blank control group and red ox Control group (P<0.01)；The hepatic glycogen content of 1 group of the basic, normal, high dosage of test group and the side of tearing open is apparently higher than blank control group and red Ox control group.As it can be seen that violent fortune has also been effectively relieved while helping to improve mouse hepatic glycogen reserve in test group drinks The dynamic protein metabolism occurred.

3. conclusion

3.1 above-mentioned experiments show, the functional composition mouse hypoxia-bearing capability that the embodiment of the present disclosure provides and blank pair It is compared according to group, death time difference extremely significantly (P<0.01).The hypoxia-bearing capability of mouse can be improved by illustrating two kinds of drinks, Can enhance the function that body resists this poor environment of anoxic, and test group hypoxia-bearing capability be significantly better than it is other.

3.2 by 3~table of table 5 as it can be seen that in a series of antifatigue experiments, the functional composition experimental result of the disclosure is The positive, and difference extremely significantly (P<0.01).By table 3 as it can be seen that in Loaned swimming test, walking weight load is considerably longer than blank Control group, and while the hypoxia-bearing capability of test group be significantly better than it is other.By table 4 as it can be seen that the functional composition of the disclosure is small The accumulation of blood lactase acid is significantly lower than blank control group (P in mouse body<0.01) aerobic capacity, can be obviously increased, breast is promoted Acid metabolic.As seen from Table 5, the functional composition mouse of the disclosure is after swimming, and internal hepatic glycogen is obviously improved, and test group Higher than blank control group, red ox control group and 2 groups of the side of tearing open (P<0.01), serum urea nitrogen level significantly lower than blank control group, Red ox control group and 2 groups of the side of tearing open (P<0.01), display test group group drinks can increase the storage level of hepatic glycogen, hepatic glycogen storage Standby increase can maintain the blood glucose level of long period, and more energy are provided for body, reduce glycogen after intense physical labour Consumption, to promote physical efficiency, enhancing endurance, antifatigue effect.

3.3 during above-mentioned experiment, and the functional composition of the disclosure is applied does not occur ill-effect in mouse.

3.4 according to experimental result, shows that the functional composition of the disclosure has the function of having resist oxygen lack, antifatigue, and And the effect of test group be substantially better than it is other.

Obviously, those skilled in the art can carry out the disclosure essence of the various modification and variations without departing from the disclosure God and range.In this way, if these modifications and variations of the disclosure belong to the range of disclosure claim and its equivalent technologies Within, then the disclosure is also intended to include these modifications and variations.

Claims

1. a kind of functional composition, it is characterised in that including basic ingredient, the basic ingredient includes salinity, vitamin, sugar Point；Functional component, including the first functional component and/or the second functional component, first functional component include Sessileflower Acanthopanax Bark, Huang Essence, matrimony vine；The second composition includes soya-bean polypeptides, olive polyphenol.

2. functional composition according to claim 1, it is characterised in that in the basic ingredient, the dosage of each component Number is 0.5-3 parts of salinity, 0.005-0.03 parts of vitamin, 5-10 parts of sugar.

3. functional composition according to claim 1, it is characterised in that in first functional component, each component Dosage number is 2-5 parts of Sessileflower Acanthopanax Bark, 8-15 parts of sealwort, 8-15 parts of matrimony vine.

4. functional composition according to claim 1, it is characterised in that in second functional component, each component Dosage number is 1-3 parts of soya-bean polypeptides, 0.05-0.3 parts of olive polyphenol.

5. functional composition according to claim 1, it is characterised in that including basic ingredient, the first functional component and The dosage number of two functional components, each component is 1 part of salinity, 0.015 part of vitamin, 8 parts of sugar, 4 parts of Sessileflower Acanthopanax Bark, sealwort 10 Part, 10 parts of matrimony vine, 2 parts of soya-bean polypeptides, 0.1 part of olive polyphenol.

6. functional composition according to claim 1, it is characterised in that the salinity includes sea salt, the vitamin packet Vitamin B2 and/or vitamin B6 are included, the sugar includes glucose.

7. functional composition described in claim 1 is preparing antifatigue drug, health products or food as active constituent In application.

8. functional composition described in claim 1 is as active constituent in the drug, health products or food for preparing resist oxygen lack In application.

9. application according to claim 7 or 8, it is characterised in that the drug, health products or food are the oral solution bodily form The mass-volume concentration of formula, functional composition is 0.1-0.2g/ml.

10. application according to claim 9, it is characterised in that the drug, health products or food are Oral liquid forms, Dosage is 1-100ml/kg.