CN108181148A - The tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method - Google Patents
The tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method Download PDFInfo
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- CN108181148A CN108181148A CN201711421907.5A CN201711421907A CN108181148A CN 108181148 A CN108181148 A CN 108181148A CN 201711421907 A CN201711421907 A CN 201711421907A CN 108181148 A CN108181148 A CN 108181148A
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- guided percutaneous
- glass slide
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Abstract
The invention belongs to in-vitro diagnosis method fields, and in particular to a kind of tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method, including following preparation process：1) CT guided percutaneous transthoracic biopsies obtain biopsy；2) tabletting method is organized；The tissue of selection is laid on a glass slide by the fresh biopsy obtained with sharp cutter fine segmentation step 1), is superimposed thereon using another glass slide and gently pressed；3) Wright's staining provides instant result；4) analysis result, guidance operate in next step.The present invention overcomes CT guided percutaneous transthoracic biopsies there are the limitation of certain false negative rate, provide it is a kind of it is easy to operate, can live instant evaluation liver mass method.
Description
Technical field
The invention belongs to in-vitro diagnosis method fields, and in particular to a kind of tissue pressure of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies
Piece and field evaluation method.
Background technology
CT guided percutaneous transthoracic biopsies are as a kind of external Biopsy method, since wound is small, and extensive use.But because
It, may also there are a degree of false negative for lower operation cannot be looked at straight completely.With regard to needing research one kind that can effectively improve work
The method for examining quality can effectively improve positive rate, reduce patient suffering, accelerate diagnosis process.Since biopsy amount is opposite
It is less, subsequent pathological diagnosis is had been unable to meet for the tissue mass after Tissue cell smears, so traditional Tissue cell smears can only be
It is used in specimens from pri.Enough, good histological specimen, accurate histological classification are obtained by safety, minimally invasive means
And molecular Biological Detection is direction and the urgent problem to be solved of Medical development.
Invention content
The purpose of the present invention is to overcome the defects in the prior art, provides a kind of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies
Organize tabletting and field evaluation method
The present invention to achieve the above object, using following technical scheme：
A kind of tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method, including following preparation process：
1) CT guided percutaneous transthoracic biopsies obtain biopsy；
2) tabletting method is organized；The fresh biopsy obtained with sharp cutter fine segmentation step 1), by selection
Tissue is laid on a glass slide, is superimposed thereon using another glass slide and gently pressed；
3) Wright's staining provides instant result；
4) analysis result, guidance operate in next step；Analysis result judges this aspiration biopsy quality, such as satisfied, stops wearing
Thorn；As being unsatisfied with, continue same position or different parts puncture again.
Preferably, the glass slide described in step 2) is the glass slide of warm, and temperature is 25-35 DEG C.With what is slightly heated
Glass slide prepares printingout, can obtain compared with many cells.
Wright's staining wherein in step 3) uses following step：3A) smear is spontaneously dried；3B) by smear in Rui Shi
1min is dyed in liquid；Continue to infiltrate 5min in phosphate buffer after 3C) taking out；3D) deionized water is dried up after rinsing with ear washing bulb,
Tentatively judged under microscope.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention overcomes CT guided percutaneous transthoracic biopsies there are the limitation of certain false negative rate, provides a kind of operation letter
It is single, can live instant evaluation liver mass method, using CT guided percutaneous needle biopsy tissues, printed on the glass slide of warm
Piece, it is easy to operate, effectively improve the success rate of CT Conducted Punctures.Reduce false negative rate, and minimal loss biopsy group
It knits, reliable guarantee is provided for clinic follow-up work.
Description of the drawings
Fig. 1 is the result schematic diagram of live tabletting in the embodiment of the present invention.
Specific embodiment
In order to which those skilled in the art is made to more fully understand technical scheme of the present invention, below in conjunction with the accompanying drawings and most
The present invention is described in further detail for good embodiment.
A kind of tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method, including following preparation process：
1) CT guided percutaneous transthoracic biopsies obtain biopsy；
2) tabletting method is organized；The fresh biopsy obtained with sharp cutter fine segmentation step 1), by selection
Tissue is laid on the glass slide of a warm, is superimposed thereon using the glass slide of another Zhang Wenre and gently pressed；Glass slide
Temperature prepare printingout with the glass slide slightly heated for 25-35 DEG C, can obtain compared with many cells.
3) Wright's staining provides instant result；Wright's staining uses following step：3A) smear is spontaneously dried；It will 3B) apply
Piece dyes 1min in Rui Shi liquid；Continue to infiltrate 5min in phosphate buffer after 3C) taking out；With washing after 3D) deionized water is rinsed
Ear ball dries up, and is tentatively judged under microscope.Fig. 1 shows the coloration result of live tabletting (small cell carcinoma).
4) analysis result, guidance operate in next step；Analysis result judges this aspiration biopsy quality, such as satisfied, stops wearing
Thorn；As being unsatisfied with, continue same position or different parts puncture again.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method, which is characterized in that including as follows
Preparation process：
1) CT guided percutaneous transthoracic biopsies obtain biopsy；
2) tabletting method is organized；The fresh biopsy obtained with sharp cutter fine segmentation step 1), by the tissue of selection
It is laid on a glass slide, is superimposed thereon using another glass slide and gently presses；
3) Wright's staining provides instant result；
4) analysis result, guidance operate in next step；Analysis result judges this aspiration biopsy quality, such as satisfied, stops puncturing；
As being unsatisfied with, continue same position or different parts puncture again.
2. the tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies according to claim 1 and field evaluation method, special
Sign is that the glass slide described in step 2) is the glass slide of warm, and temperature is 25-35 DEG C.
3. the tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies according to claim 1 and field evaluation method, special
Sign is that the Wright's staining in step 3) uses following step：3A) smear is spontaneously dried；3B) smear is contaminated in Rui Shi liquid
Color 1min；Continue to infiltrate 5min in phosphate buffer after 3C) taking out；3D) deionized water is dried up after rinsing with ear washing bulb, microscope
It is lower tentatively to be judged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711421907.5A CN108181148A (en) | 2017-12-25 | 2017-12-25 | The tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711421907.5A CN108181148A (en) | 2017-12-25 | 2017-12-25 | The tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method |
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| CN108181148A true CN108181148A (en) | 2018-06-19 |
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| CN201711421907.5A Pending CN108181148A (en) | 2017-12-25 | 2017-12-25 | The tissue tabletting of CT Guided Percutaneous Transthoracic Needle Aspiration Biopsies and field evaluation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103558150A (en) * | 2013-09-18 | 2014-02-05 | 石河子大学 | Cervical carcinoma screening method |
| CN103869057A (en) * | 2012-12-13 | 2014-06-18 | 泰州医药城博奥邦科生物科技有限公司 | Needle biopsy tissue chip |
| CN107075559A (en) * | 2014-09-29 | 2017-08-18 | 深圳华大基因股份有限公司 | The pre-treating method of clinical micro biopsy paraffin-embedded tissue |
| CN107374678A (en) * | 2017-08-15 | 2017-11-24 | 陈晓阳 | A kind of virtual positioning CT guiding lower lung biopsy methods |
-
2017
- 2017-12-25 CN CN201711421907.5A patent/CN108181148A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103869057A (en) * | 2012-12-13 | 2014-06-18 | 泰州医药城博奥邦科生物科技有限公司 | Needle biopsy tissue chip |
| CN103558150A (en) * | 2013-09-18 | 2014-02-05 | 石河子大学 | Cervical carcinoma screening method |
| CN107075559A (en) * | 2014-09-29 | 2017-08-18 | 深圳华大基因股份有限公司 | The pre-treating method of clinical micro biopsy paraffin-embedded tissue |
| CN107374678A (en) * | 2017-08-15 | 2017-11-24 | 陈晓阳 | A kind of virtual positioning CT guiding lower lung biopsy methods |
Non-Patent Citations (3)
| Title |
|---|
| A. FASSINA, ET AL.: "Role and accuracy of rapid on-site evaluation of CT-guided fine needle aspiration cytology of lung nodules", 《CYTOPATHOLOGY》 * |
| 余永强等: "《现代医院诊疗常规 医技分册》", 30 September 2012, 安徽科学技术出版社 * |
| 阮庆大 等: "CT导引技术在肺外周肿块穿刺细胞学检查中的临床应用", 《中国医药指南》 * |
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Application publication date: 20180619 |
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