CN107749936A - A kind of imaging system for embryo, egg mother cell or stem cell - Google Patents
A kind of imaging system for embryo, egg mother cell or stem cell Download PDFInfo
- Publication number
- CN107749936A CN107749936A CN201710944970.0A CN201710944970A CN107749936A CN 107749936 A CN107749936 A CN 107749936A CN 201710944970 A CN201710944970 A CN 201710944970A CN 107749936 A CN107749936 A CN 107749936A
- Authority
- CN
- China
- Prior art keywords
- disk
- embryo
- stem cell
- egg mother
- imaging system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000130 stem cell Anatomy 0.000 title claims description 59
- 210000001161 mammalian embryo Anatomy 0.000 title claims description 33
- 238000003384 imaging method Methods 0.000 title claims description 32
- 230000003287 optical effect Effects 0.000 claims description 20
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 238000013519 translation Methods 0.000 claims description 5
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims 1
- 240000002853 Nelumbo nucifera Species 0.000 claims 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 210000002257 embryonic structure Anatomy 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N17/00—Diagnosis, testing or measuring for television systems or their details
- H04N17/002—Diagnosis, testing or measuring for television systems or their details for television cameras
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
- H04N23/54—Mounting of pick-up tubes, electronic image sensors, deviation or focusing coils
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
- H04N23/55—Optical parts specially adapted for electronic image sensors; Mounting thereof
Landscapes
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Signal Processing (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to one kind to be used for embryo, egg mother cell or the imaging system of stem cell, including disk, some pallets being arranged on the disk, and the optical system taken pictures for some pallets, the disk is provided with some dividing plates from its center of circle is outwards radial, and some dividing plates are uniformly arranged, the disk is separated into some sector regions by some dividing plates, some pallets are uniformly arranged in some sector regions respectively, each pallet is evenly arranged with some depressions, the optical system includes light source successively, beam condensing unit, object lens, with charge coupling device imaging sensor.The imaging system for being used for embryo, egg mother cell or stem cell can carry out evaluation of taking pictures to multiple embryos, egg mother cell or stem cell simultaneously, save the time, highly efficient.
Description
Technical field
The present invention relates to a kind of biotechnology medical auxiliary equipment, more particularly to one kind to be used for embryo, egg mother cell or dry
The imaging system of cell.
Background technology
In Past 30 Years, auxiliary procreation technology (ART) revolutionizes the mankind sterile (infertility) treatment,
And become universal.However, ART needs to use multiple embryos, multiple embryos result in frequently-occurring multifetation, so as to
So that the death rate and pain of baby and mother greatly increase, and produce very big financial cost.
The low success rate of one of the main reasons of ART is to lack the method for being used for evaluating embryo quality.Use at present
In prediction embryo quality most reliable method be that embryo morphology is entered using standard transmitted light microscopic system before transfer
Capable inspection of taking pictures.Between generally on embryo's pallet of a certain circumference of top surface provided with an annular of a certain disk, embryo's pallet
Every being provided with some embryos, by being taken a picture some embryos to evaluate the quality of some embryos.However, such one
The evaluated embryo's negligible amounts of secondary operation, it is less efficient.
The content of the invention
The purpose of the present invention is to be directed to above-mentioned present situation, there is provided it is a kind of multiple embryos are evaluated efficient be used for embryo
Tire, egg mother cell or the imaging system of stem cell.
The technical solution adopted by the present invention：A kind of imaging system for embryo, egg mother cell or stem cell, including circle
Disk, some pallets being arranged on the disk and the optical system taken pictures for some pallets, often
One pallet is evenly arranged with some be recessed for carrying embryo, egg mother cell or stem cell, and each loading
The center and the center of circle of the disk of some depressions on pallet are located along the same line, and the disk rotates around its center of circle, institute
State optical system includes light source, beam condensing unit, object lens and charge coupling device imaging sensor, the beam condensing unit, circle successively
Disk, object lens and charge coupling device imaging sensor are successively set on the optical path of the light source, when the disk does not revolve
When turning, the straight line relative translation of the optical system and disk along the center of circle by the disk；When the disc rotary, institute
State optical system and disk is relatively motionless.
The present invention effect be：This be used for embryo, egg mother cell or stem cell imaging system can simultaneously to multiple embryos,
Egg mother cell or stem cell carry out evaluation of taking pictures, and have saved the time, highly efficient.
Brief description of the drawings
Fig. 1 is a kind of structural representation of imaging system for embryo, egg mother cell or stem cell provided by the invention.
Fig. 2 is the top view of disk in Fig. 1.
Embodiment
The principle and feature of the present invention are described below in conjunction with accompanying drawing, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Referring to Fig. 1, the structure for a kind of imaging system for embryo, egg mother cell or stem cell provided by the invention
Schematic diagram.This be used for embryo, egg mother cell or stem cell imaging system couple with outer computer, so as to the calculating
Machine communicates, and has the software for being used for analyzing the image obtained by the imaging system in the computer.
The imaging system for being used for embryo, egg mother cell or stem cell includes disk 1, some loads being arranged on disk 1
Thing pallet 2 and the optical system 3 taken pictures for pallet 2.
Please refer to Fig. 2, disk 1 is provided with some dividing plates 11, and some dividing plates 11 from its center of circle is outwards radial
It is uniformly arranged.Disk 1 is separated into some sector regions by some dividing plates 11, and disk 1 is all provided with the middle part of each sector region
There is a groove 12.The each growth of groove 12 strip, and the direction from close to the circle centre position of disk 1 to away from the center of circle is extended, from
And make some grooves 12 from the outwards radial setting of the center of circle of disk 1, and some grooves 12 are uniformly arranged.In the present embodiment
In, dividing plate 11 and groove 12 have 12.
Disk 1 rotates around its center of circle, and the angle rotated every time is relevant with the quantity of dividing plate 11.That is the quantity of dividing plate 11 is
During N, the angle that disk 1 rotates every time is 360/N degree.In the present embodiment, the angle that disk 1 rotates every time is 30 degree.
Pallet 2 is arranged in the groove 12 of disk 1, for carrying some embryos, egg mother cell or stem cell.If should
Dry pallet 2 is from the outwards radial setting of the center of circle of disk 1, and some pallets 2 are uniformly arranged.Each pallet
2 are evenly arranged with some circular depressions 21, and the center of some depressions 21 on each pallet 2 and the center of circle of disk 1
It is located along the same line.In the present embodiment, nine circular depressions 21 are evenly arranged with each pallet 2, it is each recessed
Fall into one embryo of carrying, egg mother cell or stem cell in 21.
Optical system 3 includes light source 31, beam condensing unit 32, object lens 33, refracting means 34 and charge coupling device image and passed
Sensor 35 (CCD), beam condensing unit 32, disk 1, object lens 33, refracting means 34 and charge coupling device imaging sensor 35 are successively
It is arranged in the light path of light source 31.
Light source 31 can be LED light source.
Beam condensing unit 32 is arranged on the underface of light source 31.Beam condensing unit 32 is condenser, for caused by light source 31
Light is converged, because disk 1 is arranged on the underface of beam condensing unit 32, so as to which beam condensing unit 32 makes the light of light source 31 exist
Pooled a bit on disk 1.
Object lens 33 are arranged on the lower section of disk 1, and corresponding with beam condensing unit 32.
Refracting means 34 are arranged on the lower section of object lens 33, and corresponding with object lens 33.
Charge coupling device imaging sensor 35 is arranged on the side of refracting means 34, and the refraction of corresponding refracting means 34
Face.
It is to be appreciated that whole imaging system can also be not provided with refracting means 34, the settings of refracting means 34 be in order to
Shorten the vertical distance of whole imaging system.
In addition, optical system 3, as an entirety, when disk 1 rotates, optical system 3 and disk 1 are relatively motionless.When
When disk 1 does not rotate, specifically, there can be following two modes along the straight line relative translation in the center of circle by disk 1：Disk 1 is not
Translation, straight line of the optical system 3 along the center of circle by disk 1 are translated；Optical system 3 does not translate, and disk 1 is along passing through disk
The straight line in 1 center of circle is translated.
During concrete operations, optical system 3 is first to the embryo in first depression 21 on one of pallet 2, ovum
Mother cell or stem cell are taken pictures, now translation round disk 1 or optical system 3, successively to other depressions on the pallet 2
21 are taken pictures.After the completion of all depressions 21 on the pallet 2 are taken pictures, rotated to an angle (the angle around the center of circle of disk 1
Spend pre-set) after, all depressions 21 on adjacent another pallet 2 are taken pictures, successively repeatedly, until disk
Embryo, egg mother cell or the stem cell in all depressions 21 on 1 is taken pictures untill completion.
In this way, this be used for embryo, egg mother cell or stem cell imaging system can simultaneously to multiple embryos, egg mother cell or
Stem cell carries out evaluation of taking pictures, and has saved the time, highly efficient.
It is to be appreciated that the presence or absence of dividing plate 11, the quantity of dividing plate 11, disk 1 rotate every time angle, each pallet
The quantity of depression 21 on 2 does not limit, and can be adjusted according to actual conditions.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (10)
- A kind of 1. imaging system for embryo, egg mother cell or stem cell, it is characterised in that：Including disk, be arranged on it is described Some pallets on disk and the optical system taken pictures for some pallets, each loading support Disk is evenly arranged with some be recessed and is used to carry embryo, egg mother cell or stem cell, and some on each pallet The center of depression and the center of circle of the disk are located along the same line, the disk around its center of circle rotate, the optical system according to It is secondary including light source, beam condensing unit, object lens and charge coupling device imaging sensor, the beam condensing unit, disk, object lens and electricity Lotus coupled device imaging sensor is successively set on the optical path of the light source, when the disk does not rotate, the light The straight line relative translation of system and disk along the center of circle by the disk；When the disc rotary, the optical system It is relatively motionless with disk.
- A kind of 2. imaging system for embryo, egg mother cell or stem cell as claimed in claim 1, it is characterised in that：Institute State disk and be provided with some dividing plates from its center of circle is outwards radial, and some dividing plates are uniformly arranged, some dividing plates will The disk is separated into some sector regions, and some pallets are uniformly arranged in some sector regions respectively.
- A kind of 3. imaging system for embryo, egg mother cell or stem cell as claimed in claim 2, it is characterised in that：Institute State disk and be equipped with a groove in the middle part of each sector region, each groove is from the circle centre position close to the disk to remote The direction in the center of circle is extended, so that some grooves are from the outwards radial setting of the center of circle of the disk, and if described Dry groove is uniformly arranged, and some pallets are housed in some grooves.
- A kind of 4. imaging system for embryo, egg mother cell or stem cell as claimed in claim 3, it is characterised in that：Institute Stating dividing plate and groove has 12, and the angle that the disk rotates every time is 30 degree.
- A kind of 5. imaging system for embryo, egg mother cell or stem cell as claimed in claim 4, it is characterised in that：Often 9 depressions, one embryo of each depression carrying, egg mother cell or stem cell are evenly arranged with one pallet.
- A kind of 6. imaging system for embryo, egg mother cell or stem cell as claimed in claim 2, it is characterised in that：Institute The quantity for stating dividing plate is N, and the angle that the disk rotates every time is 360/N degree.
- A kind of 7. imaging system for embryo, egg mother cell or stem cell as claimed in claim 1, it is characterised in that：Institute Stating optical system also includes refracting means, the refracting means be arranged on the object lens and charge coupling device imaging sensor it Between.
- A kind of 8. imaging system for embryo, egg mother cell or stem cell as claimed in claim 7, it is characterised in that：Institute The underface that beam condensing unit is arranged on the light source is stated, the beam condensing unit is condenser, and the object lens are arranged on the disk Lower section, and corresponding with the beam condensing unit, the refracting means are arranged on the lower section of the object lens, and with the object lens pair Should, the charge coupling device imaging sensor is arranged on the side of the refracting means, and the folding of the corresponding refracting means Penetrate face.
- A kind of 9. imaging system for embryo, egg mother cell or stem cell as claimed in claim 1, it is characterised in that：When When the disk does not rotate, the disk is not translated, and straight line of the optical system along the center of circle by the disk is put down Move.
- A kind of 10. imaging system for embryo, egg mother cell or stem cell as claimed in claim 1, it is characterised in that：When When the disk does not rotate, the optical system is not translated, and straight line of the disk along the center of circle by the disk is put down Move.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710944970.0A CN107749936A (en) | 2017-10-12 | 2017-10-12 | A kind of imaging system for embryo, egg mother cell or stem cell |
| PCT/CN2017/106976 WO2019071648A1 (en) | 2017-10-12 | 2017-10-20 | Imaging system for embryos, oocytes or stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710944970.0A CN107749936A (en) | 2017-10-12 | 2017-10-12 | A kind of imaging system for embryo, egg mother cell or stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107749936A true CN107749936A (en) | 2018-03-02 |
Family
ID=61252503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710944970.0A Pending CN107749936A (en) | 2017-10-12 | 2017-10-12 | A kind of imaging system for embryo, egg mother cell or stem cell |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN107749936A (en) |
| WO (1) | WO2019071648A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795762A (en) * | 2018-07-25 | 2018-11-13 | 太平洋康泰科学仪器（济南）有限公司 | A kind of time difference imaging culture systems and its method |
| CN109946825A (en) * | 2019-02-15 | 2019-06-28 | 武汉互创联合科技有限公司 | A kind of bidirectional imaging system for embryo, egg mother cell and stem cell |
| CN115558593A (en) * | 2022-09-02 | 2023-01-03 | 广州市华粤行医疗科技有限公司 | A medium culture observation device and its gas path control method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800164A (en) * | 1984-07-12 | 1989-01-24 | Institut National De La Sante Et De La Recherche Medicale-Inserm | Automatic device for the analysis and cloning of cellular cultures as well as for bacteriological analysis |
| WO2002033047A2 (en) * | 1998-04-28 | 2002-04-25 | Nexgenics Bioscience Corp. | In vitro embryo culture device |
| CN1894397A (en) * | 2003-12-18 | 2007-01-10 | 株式会社日立医药 | Device for cell culture |
| CN101802166A (en) * | 2007-06-29 | 2010-08-11 | 尤尼森斯繁殖技术公司 | Device, system and method for monitoring and/or culturing microscopic objects |
| CN103517676A (en) * | 2010-09-27 | 2014-01-15 | 奥克索金股份有限公司 | Devices, methods and systems for automated imaging and evaluation of embryos, oocytes and stem cells |
| US20150343439A1 (en) * | 2014-06-02 | 2015-12-03 | Seahorse Bioscience | Single column microplate system and carrier for analysis of biological samples |
| CN105136665A (en) * | 2015-08-17 | 2015-12-09 | 宁波键一电子信息技术有限公司 | Network-based intelligent imaging analyzer for living cell culture in box |
| CN106164245A (en) * | 2014-02-03 | 2016-11-23 | 尤尼森斯繁殖技术公司 | Cultivating container device and method |
-
2017
- 2017-10-12 CN CN201710944970.0A patent/CN107749936A/en active Pending
- 2017-10-20 WO PCT/CN2017/106976 patent/WO2019071648A1/en not_active Ceased
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800164A (en) * | 1984-07-12 | 1989-01-24 | Institut National De La Sante Et De La Recherche Medicale-Inserm | Automatic device for the analysis and cloning of cellular cultures as well as for bacteriological analysis |
| WO2002033047A2 (en) * | 1998-04-28 | 2002-04-25 | Nexgenics Bioscience Corp. | In vitro embryo culture device |
| CN1894397A (en) * | 2003-12-18 | 2007-01-10 | 株式会社日立医药 | Device for cell culture |
| CN101802166A (en) * | 2007-06-29 | 2010-08-11 | 尤尼森斯繁殖技术公司 | Device, system and method for monitoring and/or culturing microscopic objects |
| CN103517676A (en) * | 2010-09-27 | 2014-01-15 | 奥克索金股份有限公司 | Devices, methods and systems for automated imaging and evaluation of embryos, oocytes and stem cells |
| CN106164245A (en) * | 2014-02-03 | 2016-11-23 | 尤尼森斯繁殖技术公司 | Cultivating container device and method |
| US20150343439A1 (en) * | 2014-06-02 | 2015-12-03 | Seahorse Bioscience | Single column microplate system and carrier for analysis of biological samples |
| CN105136665A (en) * | 2015-08-17 | 2015-12-09 | 宁波键一电子信息技术有限公司 | Network-based intelligent imaging analyzer for living cell culture in box |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795762A (en) * | 2018-07-25 | 2018-11-13 | 太平洋康泰科学仪器（济南）有限公司 | A kind of time difference imaging culture systems and its method |
| CN109946825A (en) * | 2019-02-15 | 2019-06-28 | 武汉互创联合科技有限公司 | A kind of bidirectional imaging system for embryo, egg mother cell and stem cell |
| CN115558593A (en) * | 2022-09-02 | 2023-01-03 | 广州市华粤行医疗科技有限公司 | A medium culture observation device and its gas path control method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019071648A1 (en) | 2019-04-18 |
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Application publication date: 20180302 |