CN107693482A - A kind of special anti-bacteria wet keeping wet tissue of infant - Google Patents
A kind of special anti-bacteria wet keeping wet tissue of infant Download PDFInfo
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- 230000000844 anti-bacterial effect Effects 0.000 title claims description 30
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- 238000000855 fermentation Methods 0.000 claims description 60
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- 102000004190 Enzymes Human genes 0.000 claims description 53
- 108090000790 Enzymes Proteins 0.000 claims description 53
- 229940088598 enzyme Drugs 0.000 claims description 53
- 239000000284 extract Substances 0.000 claims description 51
- 239000000463 material Substances 0.000 claims description 51
- 239000007788 liquid Substances 0.000 claims description 46
- 238000013329 compounding Methods 0.000 claims description 44
- 241000894006 Bacteria Species 0.000 claims description 33
- 238000002156 mixing Methods 0.000 claims description 33
- 229920001661 Chitosan Polymers 0.000 claims description 32
- 235000015097 nutrients Nutrition 0.000 claims description 31
- 230000001954 sterilising effect Effects 0.000 claims description 24
- 239000002002 slurry Substances 0.000 claims description 23
- 239000002994 raw material Substances 0.000 claims description 20
- 244000005700 microbiome Species 0.000 claims description 19
- 239000004745 nonwoven fabric Substances 0.000 claims description 19
- 239000004094 surface-active agent Substances 0.000 claims description 19
- 102000019280 Pancreatic lipases Human genes 0.000 claims description 17
- 108050006759 Pancreatic lipases Proteins 0.000 claims description 17
- 239000003431 cross linking reagent Substances 0.000 claims description 17
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- 239000002344 surface layer Substances 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
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- -1 alkyl poly glucoside Chemical class 0.000 claims description 8
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- DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 claims description 8
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 8
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- 238000000605 extraction Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- UMHJEEQLYBKSAN-UHFFFAOYSA-N Adipaldehyde Chemical compound O=CCCCCC=O UMHJEEQLYBKSAN-UHFFFAOYSA-N 0.000 claims description 3
- KUCOHFSKRZZVRO-UHFFFAOYSA-N terephthalaldehyde Chemical compound O=CC1=CC=C(C=O)C=C1 KUCOHFSKRZZVRO-UHFFFAOYSA-N 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 2
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 claims 1
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- 150000008131 glucosides Chemical class 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
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- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of special anti-bacteria wet keeping wet tissue of infant, belong to household chemicals technical field.The special anti-bacteria wet keeping wet tissue of infant that the present invention develops includes non-woven fabrics and stoste, wherein, stoste is by water, bacterium solution extract, surfactant, complex enzyme and fig extract composition, and bacterium solution extract is the material such as cellulose membrane as caused by the product and microbial fermentation of compounding strain fermentation decline phase is combined, in addition, fig extract is by alcohol precipitation after fig and water mixing, obtained again after water dissolves, after non-woven fabrics is mixed with stoste during preparation, ultrasonic immersing, the special anti-bacteria wet keeping wet tissue of infant is produced.While its fungistatic effect is improved, it is also significantly improved the special anti-bacteria wet keeping wet tissue of infant prepared by technical solution of the present invention for skin moisturization；In addition, the also effect with swelling and pain relieving, to skin sparing excellent.
Description
Technical field
The invention discloses a kind of special anti-bacteria wet keeping wet tissue of infant, belong to household chemicals technical field.
Background technology
With the progress of people's medical knowledge and the maturation of baby wipes product, young mothers meet certainly for maximum
Oneself baby, gradually clean the tender skin of baby with the baby wipes containing various nutritional ingredients and sterilization component.It is wet
Direct effect of the towel to skin make it that requirement of the mothers to baby wipes is more and more high.Under the promotion of such market demand,
Baby wipes are just developed with swift and violent speed.
Infant skin is delicate, vulnerable, is very different with adult skin property.First, the moisture content of skin of baby
Height, pH value are high the effect of moisture (skin without reserve)；Secondly, perspired in unit area more；In addition, baby's sebum amount is low, skin
Relatively dry.Therefore, wet tissue for baby, in addition to not having toxicity to skin, eyes, nursing and security, baby should be also especially particular about
Youngster has the characteristics that high protectiveness, high security, low excitatory with wet tissue.The formula of baby wipes is designed exclusively for baby
's.Because infant skin is very tender, the barrier function of skin is also perfect without developing, easily allergy.So wet tissue formula is all used
Distilled water, pure water, and a certain amount of skin conditioner is added, sterilization component is also advocated nonirritant.Traditional baby wipes formula
In typically contain alcohol and essence, alcohol can be skin degerming, and on the one hand essence can cover the stink of formula material, another
Aspect can also provide various fragrance effects for product.This formula has used for a long time.But research is found, because neonate
The permeability of skin is higher than adult, and due to stool and urine, the wash number of baby's buttocks is especially more, and alcohol type solvent holds
Easily take this opportunity to penetrate into skin.And alcohol and essence do very big harm to skin, their addition eventually results in skin mistake
Go the harmful effects such as elastic, coarse, dry and other are stimulated.
Therefore, it is antibacterial bad with moistening effect how to improve traditional infant's wet tissue, it is only single with cleaning effect, function
The shortcomings that, to obtain infant's wet tissue of more high combination property, it is that it is promoted and is applied to broader field, meets that industry is raw
Production demand urgent problem to be solved.
The content of the invention
The present invention solves the technical problem of：It is antibacterial bad with moistening effect for traditional infant's wet tissue, only have
There is the shortcomings that cleaning effect, function is single, there is provided a kind of special anti-bacteria wet keeping wet tissue of infant.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of special anti-bacteria wet keeping wet tissue of infant, including non-woven fabrics and stoste,
The stoste is made up of the raw material of following parts by weight：30~40 parts of water, 8~10 parts of bacterium solution extracts, 4~10 parts of tables
Face activating agent, 0.1~0.2 part of complex enzyme, 3~5 parts of fig extracts；
The specific preparation process of bacterium solution extract is：
（1）Nutrient solution is added into fermentation tank, and the compounding strain of nutrient solution quality 1~3% is added into fermentation tank, is stirred
Afterwards, constant temperature is left to ferment 25~40 days, during the fermentation, nutrient solution was added into fermentation tank every 3~5 days, obtains fermentation material；
（2）Fermentation ends are treated, grip fermentation material surface layer film, after sterilizing, dries to constant weight, obtains dried microorganism film；
（3）It is 1 in mass ratio by dried microorganism film and compounding enzyme liquid：10~1：15 mix, and after supersound process, stand enzymolysis 4
~8h, then through enzyme deactivation, material must be digested；
（4）The chitosan liquid of enzymolysis material quality 8~10% and the crosslinking agent of enzymolysis material quality 8~10% are added in expecting to enzymolysis, it is permanent
Warm 45~60min of ultrasonic reaction, filter, washing, dry and grind, obtain abrasive, as bacterium solution extract；
The special anti-bacteria wet keeping wet tissue preparation process of infant is：It is 1 in mass ratio by non-woven fabrics and stoste：10~1：12 is mixed
Close, after 2~4h of ultrasonic immersing, non-woven fabrics is taken out, encapsulate, produce the special anti-bacteria wet keeping wet tissue of infant.
It is 1 in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin that the surfactant, which is,：5：1
Configuration forms.
It is 1 in mass ratio by pancreatic lipase and keratinase that the complex enzyme, which is,：1~3：1 mixes.
The fig extract extraction process is：It is 1 in mass ratio by fig and water：10~1：Ground after 20 mixing
Into fig slurries, then filtered, filtrate is obtained, then by filtrate and absolute ethyl alcohol is 1 in mass ratio：After 1 is stirred, centrifugation point
From collecting lower sediment thing, then by lower sediment thing and water be 1 in mass ratio：10~1：15 mixing, produce fig extraction
Liquid.
The nutrient solution is made up of the raw material of following parts by weight：3~5 parts of tea seed cakes, 8~10 parts of bagasse powder, 10~15
Part brewer's wort, 2~4 parts of yeast extracts, 2~4 parts of glucose, 2~4 parts of potassium dihydrogen phosphates, 30~40 parts of biogas slurries, 800~1000 parts
Water.
It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli that the compounding strain, which is,：1：1 compounding and
Into.
The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：3~5 parts of pancreatic lipases, 4~8 portions of stomach eggs
White enzyme, 4~6 parts of trypsase, 200~220 parts of water.
The chitosan liquid configuration process is：It is 1 in mass ratio：10~1：20 gather the shell that deacetylation is 90~95%
After sugar is stirred with water, 2~4h of swelling, then heated stirring and dissolving are stood, obtains chitosan liquid.
The crosslinking agent is any one in glutaraldehyde, hexandial or terephthalaldehyde.
The beneficial effects of the invention are as follows：
（1）Technical solution of the present invention provides production by adding fig extract in wet tissue product, using fig extract
The special delicate fragrance of product, the addition of compound essence is avoided to have undesirable effect infant's body, in addition, day in fig extract
Right composition can effectively protect infant's skin, be advantageous to the breeding of skin surface probiotics, while can play the effect of swelling and pain relieving
Fruit；
（2）A small amount of complex enzyme is added in technical solution of the present invention, complex enzyme in use, can produce skin surface metabolism
Grease and keratin hydrolysis, grease hydrolysis produces glycerine and unrighted acid, and glycerine can play the effect of auxiliary moisturizing, and
Unrighted acid can play good antioxidation, aliphatic acid caused by amino acid grease hydrolysis caused by keratin hydrolysis
Emulsifying effectiveness can be played, improves the cleaning effect of wet tissue；
（3）Technical solution of the present invention contains microorganism generation by adding bacterium solution extract in wet tissue product in bacterium solution extract
Thank to caused bacteria cellulose film, good moistening effect can be played, be advantageous to skin moisture-keeping of the wet tissue after storage and use,
In addition, considered critical uses the bacterium solution extract in fermentation later stage in technical solution of the present invention, and compounded using different strain
Fermentation, the decline phase that microorganism now be in growth period, containing largely being grown to compounding microorganism in zymotic fluid
" disadvantage factor ", and disadvantage factor can be adsorbed bacteria cellulose film absorption of good performance and fix and be scattered in wet tissue product
In, the effect of long-acting bacteriostatic is stored and is played after in product, can be with bacteria cellulose film in infant's skin table after use
Face forms the moisture film with good bacteriostasis property, and is somebody's turn to do " disadvantage factor " and only the harmful bacterias such as compounding strain and fungi are played
Growth inhibition effect, human body is had no toxic side effect.
Embodiment
It is 1 in mass ratio by fig and water：10~1：Grinder is poured into 20 mixing, after grinding 45~60min, discharging,
Fig slurries are obtained, then gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：
1 mixing, after being stirred 10~15min with glass bar, centrifuge, collect lower sediment thing, and by gained lower sediment thing
It is 1 in mass ratio with water：10~1：15 mixing, are stirred 45~60min with 300~500r/min rotating speeds with agitator, obtain
Fig extract；Nutrient solution is added in fermentation tank, and the compounding strain of nutrient solution quality 1~3% is added into fermentation tank,
After being stirred 10~30min with sterilization waddy, under the conditions of temperature is 28~32 DEG C, constant temperature is left to ferment 25~40 days,
In fermentation process, the nutrient solution of material gross mass 0.6~0.8% in fermentation tank was added into fermentation tank every 3~5 days, waits to ferment
Terminate, obtain fermentation material, and fermentation material surface layer film is gripped with tweezers, and surface layer film is soaked in mass fraction as 10~20%
Sodium hydroxide solution in, after 20~30min of immersion sterilizing, obtain sterilizing film, then gained is sterilized into film drying to constant weight, obtain
Dried microorganism film；It is then 1 in mass ratio by gained dried microorganism film and compounding enzyme liquid：10~1：15 pour into flask,
And flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 45~50kHz, after 45~60min of sonic oscillation, stand enzyme
4~8h is solved, with the conditions of temperature is 75~80 DEG C, 10~15min of enzyme deactivation is incubated, material must be digested；Digest and expect to gained again
It is middle add enzymolysis material quality 8~10% chitosan liquid and enzymolysis material quality 8~10% crosslinking agent, in temperature be 55~60 DEG C,
Under the conditions of supersonic frequency is 50~55kHz, thermostatic ultrasonic reacts 45~60min, filtering, obtains filter cake, and be washed with deionized
Filter cake 3~5 times, then the filter cake after washing is transferred in baking oven, 6~8h is dried under the conditions of being 75~80 DEG C in temperature, obtains drying
Filter cake, and gained dry cake is poured into mortar, 20~30min is ground, obtains abrasive, as bacterium solution extract；By weight
Number meter, takes 30~40 parts of water successively, 8~10 parts of bacterium solution extracts, 4~10 parts of surfactants, 0.1~0.2 part of complex enzyme,
3~5 parts of fig extracts, after water and bacterium solution extract first are stirred into 10~15min with glass bar, standing swelling 3~
5h, surfactant, complex enzyme and fig extract are added, be 35~40 DEG C in temperature, rotating speed is 800~1200r/
Under the conditions of min, constant temperature is stirred 2~4h, discharging, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：10~1：12
After mixing, under the conditions of supersonic frequency is 45~60kHz, after 2~4h of ultrasonic immersing, non-woven fabrics is taken out, encapsulated, produce baby children
The special anti-bacteria wet keeping wet tissue of youngster.The surfactant is by matter by alkyl poly glucoside, lauryl betaine and Tea Saponin
Amount is than being 1：5：1 configuration forms.It is 1 in mass ratio by pancreatic lipase and keratinase that the complex enzyme, which is,：1~3：1 mixing and
Into.The nutrient solution is made up of the raw material of following parts by weight：3~5 parts of tea seed cakes, 8~10 parts of bagasse powder, 10~15 parts of malt
Juice, 2~4 parts of yeast extracts, 2~4 parts of glucose, 2~4 parts of potassium dihydrogen phosphates, 30~40 parts of biogas slurries, 800~1000 parts of water.It is described
It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli that compounding strain, which is,：1：1 compounding forms.The compound enzyme
Liquid is formed by the raw material configuration of following parts by weight：3~5 parts of pancreatic lipases, 4~8 parts of pepsins, 4~6 parts of tryptoses
Enzyme, 200~220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：10~1：20 by deacetylation be 90~
After 95% chitosan is stirred with water, 2~4h of swelling, then heated stirring and dissolving are stood, obtains chitosan liquid.The crosslinking
Agent is any one in glutaraldehyde, hexandial or terephthalaldehyde.
Example 1
It is 1 in mass ratio by fig and water：Grinder is poured into 20 mixing, after grinding 60min, discharging, obtains fig slurries, then
Gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：1 mixing, uses glass bar
After being stirred 15min, centrifuge, collect lower sediment thing, and be 1 in mass ratio by gained lower sediment thing and water：15
Mixing, is stirred 60min with 500r/min rotating speeds with agitator, obtains fig extract；Nutrient solution is added in fermentation tank,
And the compounding strain of nutrient solution quality 3% is added into fermentation tank, after being stirred 30min with sterilization waddy, in temperature be 32 DEG C
Under the conditions of, constant temperature is left to ferment 40 days, during the fermentation, material gross mass in fermentation tank is added into fermentation tank every 5 days
0.8% nutrient solution, treats fermentation ends, obtains fermentation material, and grips fermentation material surface layer film with tweezers, and surface layer film is soaked
In the sodium hydroxide solution that mass fraction is 20%, after immersion sterilizing 30min, sterilizing film is obtained, then gained sterilizing film is done
It is dry to constant weight, obtain dried microorganism film；It is then 1 in mass ratio by gained dried microorganism film and compounding enzyme liquid：15 pour into burning
In bottle, and flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 50kHz, after sonic oscillation 60min, stand enzymolysis
8h, with the conditions of temperature is 80 DEG C, enzyme deactivation 15min is incubated, material must be digested；Enzymolysis material matter is added into gained enzymolysis material again
The chitosan liquid of amount 10% and the crosslinking agent of enzymolysis material quality 10%, in temperature be 60 DEG C, it is permanent under the conditions of supersonic frequency is 55kHz
Warm ultrasonic reaction 60min, filtering, obtains filter cake, and filter cake is washed with deionized 5 times, then the filter cake after washing is transferred into baking oven
In, 8h is dried under the conditions of being 80 DEG C in temperature, obtains dry cake, and gained dry cake is poured into mortar, 30min is ground,
Obtain abrasive, as bacterium solution extract；Count in parts by weight, take 40 parts of water, 10 parts of bacterium solution extracts, 10 parts of surface-actives successively
Agent, 0.2 part of complex enzyme, 5 parts of fig extracts, after water and bacterium solution extract first are stirred into 15min with glass bar, stand
5h is swelled, adds surfactant, complex enzyme and fig extract, is 40 DEG C in temperature, rotating speed is 1200r/min conditions
Under, constant temperature is stirred 4h, discharging, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：After 12 mixing, in supersonic frequency
Under the conditions of 60kHz, after ultrasonic immersing 4h, non-woven fabrics is taken out, encapsulates, produces the special anti-bacteria wet keeping wet tissue of infant.It is described
It is 1 in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin that surfactant, which is,：5：1 configuration forms.It is described
It is 3 in mass ratio by pancreatic lipase and keratinase that complex enzyme, which is,：1 mixes.The nutrient solution is by following parts by weight
Raw material composition：5 parts of tea seed cakes, 10 parts of bagasse powder, 15 portions of brewer's worts, 4 parts of yeast extracts, 4 parts of glucose, 4 parts of potassium dihydrogen phosphates, 40
Part biogas slurry, 1000 parts of water.It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli that the compounding strain, which is,：1：
1 compounding forms.The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：5 parts of pancreatic lipases, 8 parts of pepsins,
6 parts of trypsase, 220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：20 by deacetylation be 95% shell
After glycan is stirred with water, swelling 4h, then heated stirring and dissolving are stood, obtains chitosan liquid.The crosslinking agent is glutaraldehyde.
Example 2
Nutrient solution is added in fermentation tank, and the compounding strain of nutrient solution quality 3% is added into fermentation tank, is stirred with sterilization waddy
After mixing mixing 30min, under the conditions of temperature is 32 DEG C, constant temperature is left to ferment 40 days, during the fermentation, every 5 days to fermentation
The nutrient solution of material gross mass 0.8% in fermentation tank is added in tank, treats fermentation ends, obtains fermentation material, and fermentation material is gripped with tweezers
Surface layer film, and surface layer film is soaked in the sodium hydroxide solution that mass fraction is 20%, after immersion sterilizing 30min, it must go out
Bacterium film, then gained is sterilized into film drying to constant weight, obtain dried microorganism film；Then by gained dried microorganism film and compounding
Enzyme liquid is 1 in mass ratio：15 are poured into flask, and flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 50kHz,
After sonic oscillation 60min, enzymolysis 8h is stood, with the conditions of temperature is 80 DEG C, enzyme deactivation 15min is incubated, material must be digested；Again to
Gained enzymolysis material in add enzymolysis material quality 10% chitosan liquid and enzymolysis material quality 10% crosslinking agent, in temperature be 60 DEG C,
Under the conditions of supersonic frequency is 55kHz, thermostatic ultrasonic reaction 60min, filtering, filter cake is obtained, and filter cake is washed with deionized 5 times,
The filter cake after washing is transferred in baking oven again, 8h is dried under the conditions of being 80 DEG C in temperature, obtains dry cake, and gained is dried and filtered
Cake is poured into mortar, is ground 30min, is obtained abrasive, as bacterium solution extract；Count in parts by weight, take 40 parts of water successively, 10 parts
Bacterium solution extract, 10 parts of surfactants, 0.2 part of complex enzyme, water and bacterium solution extract are first stirred 15min with glass bar
Afterwards, stand swelling 5h, add surfactant, complex enzyme, in temperature be 40 DEG C, under the conditions of rotating speed is 1200r/min, constant temperature
4h is stirred, discharges, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：12 mixing after, in supersonic frequency be 60kHz
Under the conditions of, after ultrasonic immersing 4h, non-woven fabrics is taken out, encapsulates, produces the special anti-bacteria wet keeping wet tissue of infant.The surface-active
It is 1 in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin that agent, which is,：5：1 configuration forms.The complex enzyme is
It is 3 in mass ratio by pancreatic lipase and keratinase：1 mixes.The nutrient solution is the raw material group by following parts by weight
Into：5 parts of tea seed cakes, 10 parts of bagasse powder, 15 portions of brewer's worts, 4 parts of yeast extracts, 4 parts of glucose, 4 parts of potassium dihydrogen phosphates, 40 parts of biogas slurries,
1000 parts of water.It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli that the compounding strain, which is,：1：1 compounding and
Into.The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：5 parts of pancreatic lipases, 8 parts of pepsins, 6 portions of pancreas eggs
White enzyme, 220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：20 by deacetylation be 95% chitosan and water
After being stirred, swelling 4h, then heated stirring and dissolving are stood, obtains chitosan liquid.The crosslinking agent is glutaraldehyde.
Example 3
It is 1 in mass ratio by fig and water：Grinder is poured into 20 mixing, after grinding 60min, discharging, obtains fig slurries, then
Gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：1 mixing, uses glass bar
After being stirred 15min, centrifuge, collect lower sediment thing, and be 1 in mass ratio by gained lower sediment thing and water：15
Mixing, is stirred 60min with 500r/min rotating speeds with agitator, obtains fig extract；Nutrient solution is added in fermentation tank,
And the compounding strain of nutrient solution quality 3% is added into fermentation tank, after being stirred 30min with sterilization waddy, in temperature be 32 DEG C
Under the conditions of, constant temperature is left to ferment 40 days, during the fermentation, material gross mass in fermentation tank is added into fermentation tank every 5 days
0.8% nutrient solution, treats fermentation ends, obtains fermentation material, and grips fermentation material surface layer film with tweezers, and surface layer film is soaked
In the sodium hydroxide solution that mass fraction is 20%, after immersion sterilizing 30min, sterilizing film is obtained, then gained sterilizing film is done
It is dry to constant weight, obtain dried microorganism film；It is then 1 in mass ratio by gained dried microorganism film and compounding enzyme liquid：15 pour into burning
In bottle, and flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 50kHz, after sonic oscillation 60min, stand enzymolysis
8h, with the conditions of temperature is 80 DEG C, enzyme deactivation 15min is incubated, material must be digested；Enzymolysis material matter is added into gained enzymolysis material again
The chitosan liquid of amount 10% and the crosslinking agent of enzymolysis material quality 10%, in temperature be 60 DEG C, it is permanent under the conditions of supersonic frequency is 55kHz
Warm ultrasonic reaction 60min, filtering, obtains filter cake, and filter cake is washed with deionized 5 times, then the filter cake after washing is transferred into baking oven
In, 8h is dried under the conditions of being 80 DEG C in temperature, obtains dry cake, and gained dry cake is poured into mortar, 30min is ground,
Obtain abrasive, as bacterium solution extract；Count in parts by weight, take 40 parts of water, 10 parts of bacterium solution extracts, 10 parts of surface-actives successively
Agent, 5 parts of fig extracts, after water and bacterium solution extract first are stirred into 15min with glass bar, swelling 5h is stood, then add
Entering surfactant and fig extract, be 40 DEG C in temperature, under the conditions of rotating speed is 1200r/min, constant temperature is stirred 4h,
Discharging, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：After 12 mixing, under the conditions of supersonic frequency is 60kHz, ultrasound
After impregnating 4h, non-woven fabrics is taken out, encapsulates, produces the special anti-bacteria wet keeping wet tissue of infant.The surfactant is by alkyl
Poly glucoside, lauryl betaine and Tea Saponin are 1 in mass ratio：5：1 configuration forms.The nutrient solution is by following weight
The raw material composition of number：5 parts of tea seed cakes, 10 parts of bagasse powder, 15 portions of brewer's worts, 4 parts of yeast extracts, 4 parts of glucose, 4 parts of biphosphates
Potassium, 40 parts of biogas slurries, 1000 parts of water.It is described compounding strain be by staphylococcus aureus, saccharomycete and Escherichia coli in mass ratio
For 1：1：1 compounding forms.The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：5 parts of pancreatic lipases, 8 parts of stomaches
Protease, 6 parts of trypsase, 220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：Deacetylation is by 20
After 95% chitosan is stirred with water, swelling 4h, then heated stirring and dissolving are stood, obtains chitosan liquid.The crosslinking agent is
Glutaraldehyde.
Example 4
It is 1 in mass ratio by fig and water：Grinder is poured into 20 mixing, after grinding 60min, discharging, obtains fig slurries, then
Gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：1 mixing, uses glass bar
After being stirred 15min, centrifuge, collect lower sediment thing, and be 1 in mass ratio by gained lower sediment thing and water：15
Mixing, is stirred 60min with 500r/min rotating speeds with agitator, obtains fig extract；Count in parts by weight, take 40 successively
Part water, 10 parts of surfactants, 0.2 part of complex enzyme, 5 parts of fig extracts, first by water, surfactant, complex enzyme and nothing
Flowers and fruits extract solution, it is 40 DEG C in temperature, under the conditions of rotating speed is 1200r/min, constant temperature is stirred 4h, discharging, obtains stoste；Again will
Non-woven fabrics is 1 in mass ratio with stoste：After 12 mixing, under the conditions of supersonic frequency is 60kHz, after ultrasonic immersing 4h, by nonwoven
Cloth takes out, and encapsulation, produces the special anti-bacteria wet keeping wet tissue of infant.The surfactant is by alkyl poly glucoside, bay
Base glycine betaine and Tea Saponin are 1 in mass ratio：5：1 configuration forms.The complex enzyme is by matter by pancreatic lipase and keratinase
Amount is than being 3：1 mixes.The nutrient solution is made up of the raw material of following parts by weight：5 parts of tea seed cakes, 10 parts of bagasse powder, 15
Part brewer's wort, 4 parts of yeast extracts, 4 parts of glucose, 4 parts of potassium dihydrogen phosphates, 40 parts of biogas slurries, 1000 parts of water.It is described compounding strain be by
Staphylococcus aureus, saccharomycete and Escherichia coli are 1 in mass ratio：1：1 compounding forms.The compounding enzyme liquid is by following heavy
The raw material configuration of amount number forms：5 parts of pancreatic lipases, 8 parts of pepsins, 6 parts of trypsase, 220 parts of water.The chitosan liquid
Configuration process is：It is 1 in mass ratio：After 20 are stirred the chitosan that deacetylation is 95% with water, swelling 4h is stood, then
Heated stirring and dissolving, obtain chitosan liquid.The crosslinking agent is glutaraldehyde.
Example 5
It is 1 in mass ratio by fig and water：Grinder is poured into 20 mixing, after grinding 60min, discharging, obtains fig slurries, then
Gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：1 mixing, uses glass bar
After being stirred 15min, centrifuge, collect lower sediment thing, and be 1 in mass ratio by gained lower sediment thing and water：15
Mixing, is stirred 60min with 500r/min rotating speeds with agitator, obtains fig extract；Nutrient solution is added in fermentation tank,
And the compounding strain of nutrient solution quality 3% is added into fermentation tank, after being stirred 30min with sterilization waddy, in temperature be 32 DEG C
Under the conditions of, constant temperature is left to ferment 5 days, treats fermentation ends, obtains fermentation material, and grips fermentation material surface layer film with tweezers, and by table
Layer film is soaked in the sodium hydroxide solution that mass fraction is 20%, after immersion sterilizing 30min, obtains sterilizing film, then by gained
Film drying sterilize to constant weight, obtains dried microorganism film；It is in mass ratio by gained dried microorganism film and compounding enzyme liquid then
1：15 are poured into flask, and flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 50kHz, sonic oscillation 60min
Afterwards, enzymolysis 8h is stood, with the conditions of temperature is 80 DEG C, enzyme deactivation 15min is incubated, material must be digested；Again into gained enzymolysis material
The chitosan liquid of enzymolysis material quality 10% and the crosslinking agent of enzymolysis material quality 10% are added, is 60 DEG C in temperature, supersonic frequency is
Under the conditions of 55kHz, thermostatic ultrasonic reaction 60min, filtering, filter cake is obtained, and filter cake is washed with deionized 5 times, then by after washing
Filter cake be transferred in baking oven, in temperature be 80 DEG C under the conditions of dry 8h, obtain dry cake, and gained dry cake is poured into mortar
In, 30min is ground, obtains abrasive, as bacterium solution extract；Count in parts by weight, take 40 parts of water, 10 parts of bacterium solution extractions successively
Thing, 10 parts of surfactants, 0.2 part of complex enzyme, 5 parts of fig extracts, first water and bacterium solution extract are stirred with glass bar
After mixing 15min, swelling 5h is stood, adds surfactant, complex enzyme and fig extract, be 40 DEG C in temperature, turn
Under the conditions of speed is 1200r/min, constant temperature is stirred 4h, discharging, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：12
After mixing, under the conditions of supersonic frequency is 60kHz, after ultrasonic immersing 4h, non-woven fabrics is taken out, encapsulates, it is special to produce infant
Anti-bacteria wet keeping wet tissue.The surfactant is to be in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin
1：5：1 configuration forms.It is 3 in mass ratio by pancreatic lipase and keratinase that the complex enzyme, which is,：1 mixes.The nutrition
Liquid is made up of the raw material of following parts by weight：5 parts of tea seed cakes, 10 parts of bagasse powder, 15 portions of brewer's worts, 4 parts of yeast extracts, 4 portions of grapes
Sugar, 4 parts of potassium dihydrogen phosphates, 40 parts of biogas slurries, 1000 parts of water.The compounding strain is by staphylococcus aureus, saccharomycete and big
Enterobacteria is 1 in mass ratio：1：1 compounding forms.The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：5 parts
Pancreatic lipase, 8 parts of pepsins, 6 parts of trypsase, 220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：
After 20 are stirred the chitosan that deacetylation is 95% with water, swelling 4h, then heated stirring and dissolving are stood, obtains chitosan
Liquid.The crosslinking agent is glutaraldehyde.
Example 6
It is 1 in mass ratio by fig and water：Grinder is poured into 20 mixing, after grinding 60min, discharging, obtains fig slurries, then
Gained fig slurries are filtered, obtain filtrate, and are 1 in mass ratio by gained filtrate and absolute ethyl alcohol：1 mixing, uses glass bar
After being stirred 15min, centrifuge, collect lower sediment thing, and be 1 in mass ratio by gained lower sediment thing and water：15
Mixing, is stirred 60min with 500r/min rotating speeds with agitator, obtains fig extract；Nutrient solution is added in fermentation tank,
And the compounding strain of nutrient solution quality 3% is added into fermentation tank, after being stirred 30min with sterilization waddy, in temperature be 32 DEG C
Under the conditions of, constant temperature is left to ferment 15 days, during the fermentation, material gross mass in fermentation tank is added into fermentation tank every 5 days
0.8% nutrient solution, treats fermentation ends, obtains fermentation material, and grips fermentation material surface layer film with tweezers, and surface layer film is soaked
In the sodium hydroxide solution that mass fraction is 20%, after immersion sterilizing 30min, sterilizing film is obtained, then gained sterilizing film is done
It is dry to constant weight, obtain dried microorganism film；It is then 1 in mass ratio by gained dried microorganism film and compounding enzyme liquid：15 pour into burning
In bottle, and flask is moved into sonic oscillation instrument, under the conditions of supersonic frequency is 50kHz, after sonic oscillation 60min, stand enzymolysis
8h, with the conditions of temperature is 80 DEG C, enzyme deactivation 15min is incubated, material must be digested；Enzymolysis material matter is added into gained enzymolysis material again
The chitosan liquid of amount 10% and the crosslinking agent of enzymolysis material quality 10%, in temperature be 60 DEG C, it is permanent under the conditions of supersonic frequency is 55kHz
Warm ultrasonic reaction 60min, filtering, obtains filter cake, and filter cake is washed with deionized 5 times, then the filter cake after washing is transferred into baking oven
In, 8h is dried under the conditions of being 80 DEG C in temperature, obtains dry cake, and gained dry cake is poured into mortar, 30min is ground,
Obtain abrasive, as bacterium solution extract；Count in parts by weight, take 40 parts of water, 10 parts of bacterium solution extracts, 10 parts of surface-actives successively
Agent, 0.2 part of complex enzyme, 5 parts of fig extracts, after water and bacterium solution extract first are stirred into 15min with glass bar, stand
5h is swelled, adds surfactant, complex enzyme and fig extract, is 40 DEG C in temperature, rotating speed is 1200r/min conditions
Under, constant temperature is stirred 4h, discharging, obtains stoste；It is again 1 in mass ratio by non-woven fabrics and stoste：After 12 mixing, in supersonic frequency
Under the conditions of 60kHz, after ultrasonic immersing 4h, non-woven fabrics is taken out, encapsulates, produces the special anti-bacteria wet keeping wet tissue of infant.It is described
It is 1 in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin that surfactant, which is,：5：1 configuration forms.It is described
It is 3 in mass ratio by pancreatic lipase and keratinase that complex enzyme, which is,：1 mixes.The nutrient solution is by following parts by weight
Raw material composition：5 parts of tea seed cakes, 10 parts of bagasse powder, 15 portions of brewer's worts, 4 parts of yeast extracts, 4 parts of glucose, 4 parts of potassium dihydrogen phosphates, 40
Part biogas slurry, 1000 parts of water.It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli that the compounding strain, which is,：1：
1 compounding forms.The compounding enzyme liquid is formed by the raw material configuration of following parts by weight：5 parts of pancreatic lipases, 8 parts of pepsins,
6 parts of trypsase, 220 parts of water.The chitosan liquid configuration process is：It is 1 in mass ratio：20 by deacetylation be 95% shell
After glycan is stirred with water, swelling 4h, then heated stirring and dissolving are stood, obtains chitosan liquid.The crosslinking agent is glutaraldehyde.
Comparative example：The baby wipes of voluntary certain commodity Co., Ltd production.
The special anti-bacteria wet keeping wet tissue of infant of the gained of example 1 to 6 and comparative example product are subjected to performance detection, specific inspection
Survey method is as follows：
1. bacteriostasis property：Test specimen print (2cm × 3cm) is taken respectively, is placed in sterilized petri dishes, and examination is uniformly added dropwise on each print
Bacteria suspension 0.1mL is tested, acts on 5min, print is put into the test tube of the nertralizer containing 5.0mL, fully mixes simultaneously neutralization
10min；Then its fully shaking is eluted, takes eluent 0.5mL to pour into inoculation plate, pour into the nutrient agar culture of thawing
Base, mix, 37 DEG C of culture 48h are placed in after solidification, makees count plate, calculates bacteriostasis rate；Experiment is repeated 3 times；
2. performance of keeping humidity：It is lost in Tewameter TM300 skin moistures after the detection epidermis use of (TEWL) tester through epidermis
Percentage of water loss；With transepidermal water loss rate test probe measure subject's inner forearm mark point test specimen use skin after 2h through epidermis
Percentage of water loss, each test point are surveyed 20s, averaged；Transepidermal water loss rate is smaller, then performance of keeping humidity is better.
Specific testing result is as shown in table 1：
Table 1
From the testing result of table 1, its is antibacterial in raising for the special anti-bacteria wet keeping wet tissue of infant prepared by technical solution of the present invention
While effect, it is also significantly improved for skin moisturization；In addition, the also effect with swelling and pain relieving, to skin
Protecting effect is splendid, is had broad prospects in the development of commodity industry.
Claims (9)
1. a kind of special anti-bacteria wet keeping wet tissue of infant, including non-woven fabrics and stoste, it is characterised in that：
The stoste is made up of the raw material of following parts by weight：30~40 parts of water, 8~10 parts of bacterium solution extracts, 4~10 parts of tables
Face activating agent, 0.1~0.2 part of complex enzyme, 3~5 parts of fig extracts；
The specific preparation process of bacterium solution extract is：
（1）Nutrient solution is added into fermentation tank, and the compounding strain of nutrient solution quality 1~3% is added into fermentation tank, is stirred
Afterwards, constant temperature is left to ferment 25~40 days, during the fermentation, nutrient solution was added into fermentation tank every 3~5 days, obtains fermentation material；
（2）Fermentation ends are treated, grip fermentation material surface layer film, after sterilizing, dries to constant weight, obtains dried microorganism film；
（3）It is 1 in mass ratio by dried microorganism film and compounding enzyme liquid：10~1：15 mix, and after supersound process, stand enzymolysis 4
~8h, then through enzyme deactivation, material must be digested；
（4）The chitosan liquid of enzymolysis material quality 8~10% and the crosslinking agent of enzymolysis material quality 8~10% are added in expecting to enzymolysis, it is permanent
Warm 45~60min of ultrasonic reaction, filter, washing, dry and grind, obtain abrasive, as bacterium solution extract；
The special anti-bacteria wet keeping wet tissue preparation process of infant is：It is 1 in mass ratio by non-woven fabrics and stoste：10~1：12 is mixed
Close, after 2~4h of ultrasonic immersing, non-woven fabrics is taken out, encapsulate, produce the special anti-bacteria wet keeping wet tissue of infant.
A kind of 2. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The surfactant
It is 1 in mass ratio by alkyl poly glucoside, lauryl betaine and Tea Saponin to be：5：1 configuration forms.
A kind of 3. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The complex enzyme be by
Pancreatic lipase and keratinase are 1 in mass ratio：1~3：1 mixes.
A kind of 4. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The fig extraction
Liquid extraction process is：It is 1 in mass ratio by fig and water：10~1：Fig slurries are ground into after 20 mixing, then it is filtered,
Filtrate, then by filtrate and absolute ethyl alcohol be 1 in mass ratio：After 1 is stirred, centrifuge, collect lower sediment thing, then will
Lower sediment thing is 1 in mass ratio with water：10~1：15 mixing, produce fig extract.
A kind of 5. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The nutrient solution be by
The raw material composition of following parts by weight：3~5 parts of tea seed cakes, 8~10 parts of bagasse powder, 10~15 portions of brewer's worts, 2~4 parts of yeast extracts, 2
~4 parts of glucose, 2~4 parts of potassium dihydrogen phosphates, 30~40 parts of biogas slurries, 800~1000 parts of water.
A kind of 6. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：It is described compounding strain be
It is 1 in mass ratio by staphylococcus aureus, saccharomycete and Escherichia coli：1：1 compounding forms.
A kind of 7. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：It is described compounding enzyme liquid be
Formed by the raw material configuration of following parts by weight：3~5 parts of pancreatic lipases, 4~8 parts of pepsins, 4~6 parts of trypsase, 200
~220 parts of water.
A kind of 8. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The chitosan liquid is matched somebody with somebody
The process of putting is：It is 1 in mass ratio：10~1：After 20 are stirred the chitosan that deacetylation is 90~95% with water, stand molten
Swollen 2~4h, then heated stirring and dissolving, obtain chitosan liquid.
A kind of 9. special anti-bacteria wet keeping wet tissue of infant according to claim 1, it is characterised in that：The crosslinking agent is penta
Any one in dialdehyde, hexandial or terephthalaldehyde.
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| CN112842959A (en) * | 2021-01-30 | 2021-05-28 | 亿茂环境科技股份有限公司 | Formula and manufacturing process of traditional Chinese medicine and western medicine synthesized disinfection wet tissue |
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|---|---|---|---|---|
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| CN112842959B (en) * | 2021-01-30 | 2022-04-22 | 亿茂环境科技股份有限公司 | Formula and manufacturing process of traditional Chinese medicine and western medicine synthesized disinfection wet tissue |
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