CN107581159A - A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee - Google Patents

Abstract

A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee, and the cloth moths attracted by lamplight of collection worm bag of surviving the winter is placed into raising box, sprouts wings and outstanding cocoon honeybee；Collection figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae are brought into laboratory and cultivated；Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used after figured cloth moths attracted by lamplight worm's ovum turns to larva, to be put into feeding box and raise, obtain the low instar larvae of figured cloth moths attracted by lamplight as host；By outstanding cocoon honeybee into honeybee is female, male pairing, it is introduced into raising box, allows its natural mating, and the ovipositing hosts in host；Then change to the new feeding box for having not parasitic host and relay continued access honeybee, until queen bee is dead；Parasitic wasp feeds natural feed or man-made feeds, you can sprouts wings and outstanding cocoon honeybee of future generation；Cocoon honeybee can be hanged using this method by large-scale breeding indoors, and practical biological prevention means are provided for Stand control figured cloth moths attracted by lamplight or other Lepidoptera leafeating insects.

Description

A kind of artificial reproduction method of the natural enemy of the calico moth, the chrysalis

technical field

The invention relates to the field of biological control of agricultural and forestry pests, in particular to an artificial propagation method of a scutellaria parasitic on the larvae of the moth moth.

Background technique

Camptoloma interiorata (Walker) is an important leaf-eating pest against oak trees, and it is also a forest pest species with strong adaptability and outbreak. The insect is widely distributed in our country. The insect will cause serious damage to the growth of local trees and forest landscape, and seriously affect the development of local economy and tourism. At present, the means of controlling the calico moth in my country are still relatively traditional, and the artificial physical control and chemical control are the main methods in general, while the research on biological control is relatively weak. In order to explore the biological control technology of the moth moth, and to provide new ways and means for the future control of this insect. When we studied the parasitic natural enemies of the larvae of the moth moth, we found that there was a parasitic wasp with the highest parasitic rate, which should be a dominant natural enemy of the larvae of the moth moth in Northeast China. The parasitic wasp was identified as Meteorus sp. by experts. Up to the present, there is no artificial breeding technique of this chrysalis, and there are no relevant articles published for reference.

Contents of the invention

The object of the present invention is to provide a method for artificial propagation of the moth moth, which can be used to reproduce the wasp on a large scale indoors, and provide a practical method for preventing and controlling the moth or other leaf-eating pests of the order Lepidoptera in the forest. biological control techniques.

The invention provides a method for artificial reproduction of the natural enemy of the calico moth, the natural enemy of the scutellaria, which comprises the following steps:

a. Collection of bee species: In spring, collect the overwintering insect packs of the calico moth in the wild, bring them back to the laboratory, place them in a breeding box, and place them in a room with a temperature of 22-28°C, a relative humidity of 50-70%, and a natural photoperiod. Cultivated under the same conditions, the bee can be eclosion. After the adult bee emerges, use 10-15% honey water every day to supplement the nutrition for the adult bee, so as to increase the number of eggs laid and prolong the life of the adult bee. It can also be used in the second year. Directly use the imago of the calamari moth that is subcultured as the bee breeding;

b. Collect and raise the host of the wasps: In mid-to-late June, collect the egg masses or newly hatched larvae of the calico moth in the field, and bring the collected eggs of the calico moth into the laboratory for cultivation; or use the laboratory to continue The subcultured calico moth is used as the host, and 2 to 3 layers of absorbent paper are spread in the aseptic rearing box, and the eggs are put into it, and then placed in a light incubator for cultivation after airtight. The culture temperature is 22-28°C, the photoperiod L:D=14h:10h, and the relative humidity is 50-70%. After the larvae of the calico moth are hatched into larvae, the larvae of the calico moth are put into a feeding box to raise, with 10-15 larvae in each box, to obtain young larvae of the calico moth;

c. Receiving bees: Pair the female and male adult bees of the scutellaria, introduce them into the breeding box, let them mate naturally under room temperature and natural light, and lay eggs in the host to parasitize; Eggs, the bee receiving ratio is 10 heads/box, take out the host after 24 hours, the bee receiving ratio is 20 heads/box, take out the host after 48 hours, and then replace it with a new feeding box with unparasitized hosts to continue receiving bees , until the female bee dies;

d. Development management of parasitoids: take out the hosts that have been parasitized, place them at 20-28°C, photoperiod L:D=14h:10h, and cultivate them under the conditions of relative humidity of 50-70%, and feed them with natural feed or artificial feed , to emerge the next generation of chrysalis;

The positive effect that the present invention has:

1. The present invention successfully realizes the indoor artificial large-scale reproduction of the natural enemy of the moth, the scutellaria spp., so that the large-scale reproduction of the bee is not restricted by the season, can effectively improve labor productivity and equipment use efficiency, and save costs , which ensures a long-term and large supply source of parasitic wasps, and is conducive to long-term and effective biological control of the calico moth or other Lepidopteran leaf-eating pests.

2. The technical solution provided by the present invention adopts breeding and utilizes natural hosts, which is conducive to maintaining the parasitic ability of the wasps, ensuring the forest control effect, and reducing the frequency of rejuvenation of natural enemy insect populations. At the same time, artificial feed is used to raise hosts successfully and effectively It avoids the problem of difficult access to natural feed (Quercus mongolica and Quercus liaotungen) due to seasonal reasons.

3. The size of the female and male individuals of the bred wasp of the present invention is not significantly different from the size of the wild-type wasp collected in the field. In addition, there is no significant difference with the wild type in terms of life span, sex ratio, etc. . Experiments show that the progeny of the wasps propagated by the method of the present invention are very similar to the progeny of natural reproduction.

4. The artificial breeding method of the wasps of the present invention is simple and easy, especially suitable for indoor breeding and observation of their life history. Even if the breeders have no relevant professional knowledge, they can learn and operate independently in a short period of time.

The present invention will be described in further detail below through specific examples, but the present invention is not limited thereto.

detailed description

Example 1

a. In early and mid-April every spring, collect the overwintering insect packs of the calico moth in the wild Quercus liaotungensis or Quercus mongolica forest, bring them back to the laboratory, and place them in sterilized rearing boxes, (the density is 10 per box ), cultured under the conditions of temperature 22-28°C, relative humidity 50-70%, and natural photoperiod, and the bee can be eclosion. After the adult bee eclosion, add 10% honey to the absorbent cotton ball with a diameter of 1cm every day Water, so that the cotton balls are moist but no liquid seeps out, put them into the feeding box to supplement nutrition for the adult bees, so as to increase the number of eggs laid and prolong the life of the adult bees. The second year also can directly utilize the imago of the flower cloth moth that is subcultured as a kind of bee.

b. In mid-to-late June, the adult moths collected egg masses or newly hatched larvae from the wild, and brought the collected eggs into the laboratory for cultivation. Or use the calico moth subcultured in the laboratory as the host, spread 2 to 3 layers of absorbent paper in the aseptic rearing box, put the eggs in it, seal it and put it in the light incubator for cultivation. The culture temperature is 22-28°C, the photoperiod L:D=14h:10h, and the relative humidity is 50-70%. After the calico moth larvae have hatched into larvae, put the calico lamp moth larvae into a feeding box to raise them. There are 10 larvae per box, and fresh leaves of Quercus liaotungensis and Quercus mongolica are replaced every day, or fed with American white moth larvae feed (purchased and used American white moth larvae feed developed by the Chinese Academy of Forestry, patent number: 200510127628.9;), and cleaned Box feces, leftover feed, and dead larvae. Obtain the young larvae (1-2 instars) of the calico lamp moth.

c. Pair the female and male adult bees of the wasps, introduce them into the breeding box, let them mate naturally under room temperature and natural light, and lay eggs and parasitize in the host. The ratio of female bees to hosts is 1:10 (one rearing box). After 24 hours of laying eggs, the female bee wasps were taken out and the adult bees were taken out, and then replaced in a new feeding box with unparasitic hosts to continue receiving bees until the female bees died.

d. Take out the host that has been parasitized, and culture it under the conditions of 20-28°C, photoperiod L:D=14h:10h, and relative humidity of 50-70%, and replace fresh leaves of Quercus liaotungensis, Quercus mongolica or American The white moth larva feed is raised, and the feces, remaining feed, and dead larvae are cleaned up in the box. Then the next generation of wasps can be eclosion.

The feeding box is an organic plastic box with a length, width, and height of 12cm×9cm×4.5cm. There is no cover on the top of the box, and it is covered with a 30-mesh window screen.

Example 2

a. In early and mid-April every spring, collect overwintering insect packs of the calico moth from Quercus liaotungensis or Quercus mongolica in the wild, bring them back to the laboratory, and place them in sterilized rearing boxes (the density is 20 per box) , put it under the conditions of temperature 22-28℃, relative humidity 50-70%, and natural photoperiod, and the bee can emerge from the cocoon. After the adult bee emerges, add 15% honey water to the absorbent cotton ball with a diameter of 1cm every day. , so that the cotton balls are moist but no liquid seeps out, put them into the feeding box to supplement nutrition for the adult bees, so as to increase the number of eggs laid and prolong the life of the adult bees. The second year also can directly utilize the adult worms of the subculture to be used as kind of bees.

b. In mid-to-late June, the adult moths collected egg masses or newly hatched larvae from the wild, and brought the collected eggs into the laboratory for cultivation. Or use the calico moth subcultured in the laboratory as the host, spread 2 to 3 layers of absorbent paper in the aseptic rearing box, put the eggs in it, seal it and put it in the light incubator for cultivation. The culture temperature is 22-28°C, the photoperiod L:D=14h:10h, and the relative humidity is 50-70%. After the calico moth larvae have hatched into larvae, put the calico lamp moth larvae into a feeding box to raise them. There were 20 larvae in each box, and fresh Quercus liaotungensis, Quercus mongoliana young leaves or white moth larvae feed were replaced every day for rearing. And clean up the feces, remaining feed, and dead larvae in the box. Obtain the young larvae (1-2 instars) of the calico lamp moth.

c. Pair the female and male adult bees of the wasps, introduce them into the breeding box, let them mate naturally under room temperature and natural light, and lay eggs and parasitize in the host. The ratio of female bees to hosts is 1:20 (one rearing box). After 48 hours after the female bee started laying eggs, the adult wasps were taken out, and then they were replaced with new feeding boxes with unparasitic hosts to continue receiving bees until the female bees died.

d. Take out the host larvae that have been parasitized, and culture them under the conditions of 20-28°C, photoperiod L:D=14h:10h, and relative humidity of 50-70%, and replace fresh leaves of Quercus liaotungensis or Quercus mongolica every day. The American white moth larvae feed was raised, and the feces, remaining feed, and dead larvae were cleaned up in the box. Then the next generation of wasps can be eclosion.

The feeding box is an organic plastic box with a length, width, and height of 12cm×9cm×4.5cm. There is no cover on the top of the box, and it is covered with a 30-mesh window screen.

Claims (4)

1. a kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee, it is characterised in that:Comprise the following steps：

A. honeybee kind gathers：In spring, survived the winter worm bag in field acquisition figured cloth moths attracted by lamplight, take back laboratory, be placed into raising box, It is placed in 22-28 DEG C of temperature, relative humidity 50-70%, cultivated under conditions of the natural photoperiod, you can sprouts wings and outstanding cocoon honeybee, into honeybee 10% hydromel is used after emergence daily, is to be supplemented the nutrients into honeybee, to improve spawning quantity, and extension is into the life-span of honeybee；Second Year Also cocoon bee imago directly can be hanged as kind of a honeybee by the use of the figured cloth moths attracted by lamplight of squamous subculture；

B. gather and raise the host of outstanding cocoon honeybee：Mid or late June, field acquisition figured cloth moths attracted by lamplight pieces of an egg or newly hatched larvae, will be adopted The figured cloth moths attracted by lamplight worm's ovum collected is brought into laboratory and cultivated；Or the figured cloth moths attracted by lamplight of laboratory squamous subculture are used as host, 2~3 layers of blotting paper is spread in sterile raising box, worm's ovum is put into wherein, is placed in illumination box and cultivates after closed；

22~28 DEG C of cultivation temperature, photoperiod L:D=14h:10h, under conditions of relative humidity is 50-70%；

After figured cloth moths attracted by lamplight worm's ovum turns to larva, figured cloth moths attracted by lamplight larva is put into feeding box and raised, obtains the low of figured cloth moths attracted by lamplight Instar larvae；

C. honeybee is connect：By outstanding cocoon honeybee into honeybee is female, male pairing, it is introduced into raising box, under room temperature condition and natural lighting, allows it Natural mating, and the ovipositing hosts in host；Outstanding cocoon honeybee queen bee takes out outstanding cocoon honeybee into honeybee after starting spawning 24 hours, then change Continued access honeybee is relayed to the new feeding box for having not parasitic host, until queen bee is dead；

D. parasitic wasp development management：Take out by the host of parasitism, be placed in 20-28 DEG C, photoperiod L:D=14h:10h is relatively wet Spend to be cultivated under conditions of 50-70%, feed natural feed or man-made feeds, you can sprout wings and outstanding cocoon honeybee of future generation.

2. figured cloth moths attracted by lamplight according to claim 1 hang cocoon honeybee artificial fecundation method, it is characterised in that outstanding cocoon honeybee is female into honeybee Quantitative proportion with host is 1:10-20.

3. figured cloth moths attracted by lamplight according to claim 1 hang the artificial fecundation method of cocoon honeybee, it is characterised in that described queen bee into When worm starts spawning, it is 10/box to connect honeybee ratio, and host is taken out after 24 hours, and it is 20/box to connect honeybee ratio, rear after 48 hours to take out Host, a collection of new host is then changed, change a collection of host within hereafter every 24 hours or 48 hours, until queen bee is dead.

4. figured cloth moths attracted by lamplight according to claim 1 hang the artificial fecundation method of cocoon honeybee, it is characterised in that feeding box is organic Plastic casing, length, width and height size are 12cm × 9cm × 4.5cm, box top uncovered, cover 30 Muchuang gauzes.