CN107536037A - A kind of brown sugar prebiotics neokestose comprehensive enzyme and preparation method thereof - Google Patents
A kind of brown sugar prebiotics neokestose comprehensive enzyme and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to sugar cane food processing technique field, and in particular to a kind of brown sugar prebiotics neokestose comprehensive enzyme and preparation method thereof, its material includes：Brown sugar, aspergillus niger mycelia, black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn, dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, banana, resistant dextrin, navy bean and compound probiotic.Its preparation method is：（1）Other materials in addition to brown sugar, aspergillus niger mycelia and compound probiotic etc. are smashed to pieces, slurrying；（2）Brown sugar is added to mixed serum
Description
Technical field
The invention belongs to sugar cane food processing technique field, and in particular to a kind of brown sugar prebiotics neokestose comprehensive enzyme
And preparation method thereof.
Background technology
Neokestose is a kind of ketose race FOS, is had compared with the more preferable growth-promoting probiotics of 1F- FOSs
Ability, it is referred to as " efficient prebiotics ", while is also a kind of new type functional sweetener.And it is prebiotic that brown sugar is prepared with brown sugar
First neokestose, there is the function of prebiotics, also maintain the nutritional ingredient of brown sugar.Ferment is the health care production newly risen
Product, because ferment there are the functions such as beauty, net blood, fat-reducing, removing free radical, toxin expelling, favored by consumers in general, be even more in recent years
Sweep the country.Enterprise, R＆D institution put into a huge sum of money to develop in the research and development of ferment product one after another, and the time has emerged in large numbers many ferment
Product.It is contemplated that for ferment market supplement it is a kind of with black food nutrition feature, have the functional characteristics of ferment and prebiotic
The brown sugar prebiotics neokestose comprehensive enzyme product of meta function feature.
The content of the invention
It is an object of the present invention to it is a kind of function that there is black food nutrition feature, have ferment of ferment market supplement
The brown sugar prebiotics neokestose comprehensive enzyme product of feature and prebiotics functional characteristics.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of brown sugar prebiotics neokestose comprehensive enzyme, is mainly made up of the material of following parts by weight：It is brown sugar 31-37 parts, black
Aspergillus hyphae 0.2-0.5 parts, black grape 2-3 parts, Rosa roxburghii 1.5-2 parts, black mulberries 3.8-4.6 parts, black soya bean 2-3 parts, black glutinous rice 2-
2.5 parts, Semen sesami nigrum 1-1.5 parts, black fungus 0.8-1.2 parts, blank corn 5-7 parts, dateplum persimmon 0.7-1.2 parts, rye 5-8 parts, black dragon
Eye core 1.1-1.4 parts, black semen litchi 2.2-3.4 parts, Angelica Keiskei 6-11 parts, Exocarpium Citri Rubrum tire fruit 0.6-0.9 parts, walnut kernel 0.3-0.6
Part, fructus cannabis 1.2-1.6 parts, old ginger 1-2 parts, pawpaw 4-6 parts, dragon fruit 2-5 parts, banana 2-4 parts, resistant dextrin 10-13 parts,
Navy bean 2-2.8 parts and compound probiotic 1.6-1.8 parts.
In the present invention, further instruction, the brown sugar prebiotics neokestose comprehensive enzyme is mainly by following weight
The material of part is made：35 parts of brown sugar, 0.4 part of aspergillus niger mycelia, 2.5 parts of black grape, 1.8 parts of Rosa roxburghii, 4 parts of black mulberries, black soya bean 2.5
Part, 2.3 parts of black glutinous rice, 1.3 parts of Semen sesami nigrum, 1 part of black fungus, 6 parts of blank corn, 1 part of dateplum persimmon, 7 parts of rye, 1.2 parts of black longan seed,
Black 2.8 parts of semen litchi, 8 parts of Angelica Keiskei, 0.7 part of Exocarpium Citri Rubrum tire fruit, 0.5 part of walnut kernel, 1.4 parts of fructus cannabis, 1.5 parts of old ginger, pawpaw 5
Part, 3 parts of dragon fruit, 3 parts of banana, 11 parts of resistant dextrin, 2.4 parts of navy bean and 1.7 parts of compound probiotic.
In the present invention, further instruction, the compound probiotic include Lactobacillus rhamnosus, bifidobacterium adolescentis,
Long discrimination Bifidobacterium, Lactobacillus plantarum, lactobacillus fermenti, lactobacillus bulgaricus, bifidobacterium breve, bifidobacterium infantis and thermophilic
Lactobacillus lactis.
In the present invention, further instruction, it is the black grape, the Rosa roxburghii, the black mulberries, the black soya bean, described
Black fungus, the blank corn, the Angelica Keiskei, the pawpaw, the dragon fruit, part banana and the navy bean are new
Fresh wet feed.
In the present invention, further instruction, it is the brown sugar, the black glutinous rice, the Semen sesami nigrum, the dateplum persimmon, described
Rye, the black longan seed, the black semen litchi, the Exocarpium Citri Rubrum tire fruit, the walnut kernel, the fructus cannabis, the old ginger, institute
State the siccative of resistant dextrin and the compound probiotic for market sale.
A kind of method for preparing brown sugar prebiotics neokestose comprehensive enzyme, comprises the following steps：
（1）Slurrying：By weight, weigh black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn,
Dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, perfume (or spice)
Any of several broadleaf plants, resistant dextrin and navy bean, above material mixing is stirred, and 15-18min is handled with tissue mashing machine, ground with sand mill
Into all passing through the mixed serum of 20 mesh；
（2）First step microbe conversion：By weight, brown sugar and black-koji mould filament are weighed, brown sugar is added to step（1）Gained
Mixed serumIn and mix, it is 55-62% to make solid content, obtains mixed serum, adjust mixed serumTemperature extremely
65-70 DEG C, make mixed serumBe cooled to 44-50 DEG C after keeping 90-100min under conditions of being maintained at 65-70 DEG C, then toward its
Middle addition black-koji mould filament simultaneously stirs, and obtains mixed serum, make mixed serumIn 42-48 DEG C of temperature conditionss
Under carry out fermentation 68-72h, after its filtrate of leaching, obtain zymotic fluid；
（3）Second step microbe conversion：By weight, compound probiotic is weighed, and adds it to step（2）Obtained zymotic fluid
In, stirring mixes both, obtains mixed serum, then by mixed serumSealing, is placed in being fermented under normal temperature, ferments
Unpacking obtains brown sugar prebiotics neokestose comprehensive enzyme after 150-180 days.
The present invention has the advantage that compared with prior art：
1. product of the present invention is not only nutritious, also the growth-promoting probiotics work(of the nutritive effect with black-food, neokestose
The health-care efficacy of effect and ferment, is a kind of product for integrating multiple efficacies.Black-food is nutritious, wherein big containing three
Nutrient, vitamin, trace element and abundant melanin class thing, have good health-care efficacy, the material of product of the present invention
Most black edible raw material is used in material, has continued the nutritional activities of black-food raw material.Neokestose has oligomeric compared with 1F-
The more preferable growth-promoting probiotics ability of fructose, is referred to as " efficient prebiotics ", at the same be also a kind of new type functional sweetener, this hair
It is bright that prebiotics neokestose is changed into using brown sugar, there is the function of prebiotics, also maintain the nutritional ingredient of brown sugar.Ferment can
Improve invading for the dynamic equilibrium of internal enteric microorganism, the metabolic function of promotion digestive system, suppression harmful bacteria and toxin
Enter, further assist various nutrients to be absorbed by human body, build the excellent environment that probiotics breeds in vivo, it is strong so as to reach
Body is healthy and strong, the good result of beautiful appearance, and the present invention ensure that product enzyme activity thing using composite probiotics ferment production ferment
Risk that is pure, avoiding spontaneous fermentation miscellaneous bacteria from staining.
2. the material source used in the preparation method of product of the present invention is wide, preparation method is simple, and equipment used is normal
Equipment is advised, suitable for large-scale popularization.
Embodiment
The invention provides a kind of preparation method of brown sugar prebiotics neokestose comprehensive enzyme is provided, to make the present invention's
Purpose, technical scheme and effect are clearer, clear and definite, and the present invention is described in more detail below.It should be appreciated that this place is retouched
The specific embodiment stated only to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of brown sugar prebiotics neokestose comprehensive enzyme provided by the invention and preparation method thereof is as follows：
A kind of brown sugar prebiotics neokestose comprehensive enzyme, is mainly made up of the material of following parts by weight：31 parts of brown sugar, aspergillus niger
0.2 part of mycelia, 2 parts of black grape, 1.5 parts of Rosa roxburghii, 3.8 parts of black mulberries, 2 parts of black soya bean, 2 parts of black glutinous rice, 1 part of Semen sesami nigrum, black fungus
0.8 part, 5 parts of blank corn, 0.7 part of dateplum persimmon, 5 parts of rye, 1.1 parts of black longan seed, 2.2 parts of black semen litchi, 6 parts of Angelica Keiskei, Exocarpium Citri Rubrum
0.6 part of tire fruit, 0.3 part of walnut kernel, 1.2 parts of fructus cannabis, 1 part of old ginger, 4 parts of pawpaw, 2 parts of dragon fruit, 2 parts of banana, resistant dextrin
1.6 parts of 10 parts, 2 parts of navy bean and compound probiotic.
The present invention also provides the preparation method of brown sugar prebiotics neokestose comprehensive enzyme described above, including following step
Suddenly：
（1）Slurrying：By weight, weigh black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn,
Dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, perfume (or spice)
Any of several broadleaf plants, resistant dextrin and navy bean, above material mixing is stirred, and 15min is handled with tissue mashing machine, worn into entirely with sand mill
The mixed serum that portion passes through 20 mesh；
（2）First step microbe conversion：By weight, brown sugar and black-koji mould filament are weighed, brown sugar is added to step（1）Gained
Mixed serumIn and mix, make solid content be 55%, obtain mixed serum, adjust mixed serumTemperature to 65
DEG C, make mixed serum44 DEG C are cooled to after keeping 100min under conditions of being maintained at 65 DEG C, then is charged with black-koji mould
Filament simultaneously stirs, and obtains mixed serum, make mixed serumFermentation 68h is carried out under 42 DEG C of temperature conditionss, then
Its filtrate is filtered to take, obtains zymotic fluid；
（3）Second step microbe conversion：By weight, compound probiotic is weighed, and adds it to step（2）Obtained zymotic fluid
In, stirring mixes both, obtains mixed serum, then by mixed serumSealing, is placed in being fermented under normal temperature, ferments
Unpacking obtains brown sugar prebiotics neokestose comprehensive enzyme after 150 days.
Embodiment 2
A kind of brown sugar prebiotics neokestose comprehensive enzyme provided by the invention and preparation method thereof is as follows：
A kind of brown sugar prebiotics neokestose comprehensive enzyme, is mainly made up of the material of following parts by weight：35 parts of brown sugar, aspergillus niger
0.4 part of mycelia, 2.5 parts of black grape, 1.8 parts of Rosa roxburghii, 4 parts of black mulberries, 2.5 parts of black soya bean, 2.3 parts of black glutinous rice, 1.3 parts of Semen sesami nigrum,
1 part of black fungus, 6 parts of blank corn, 1 part of dateplum persimmon, 7 parts of rye, 1.2 parts of black longan seed, 2.8 parts of black semen litchi, 8 parts of Angelica Keiskei, tangerine
Red 0.7 part of tire fruit, 0.5 part of walnut kernel, 1.4 parts of fructus cannabis, 1.5 parts of old ginger, 5 parts of pawpaw, 3 parts of dragon fruit, 3 parts of banana, resistance
1.7 parts of 11 parts of dextrin, 2.4 parts of navy bean and compound probiotic.
The present invention also provides the preparation method of brown sugar prebiotics neokestose comprehensive enzyme described above, including following step
Suddenly：
（1）Slurrying：By weight, weigh black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn,
Dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, perfume (or spice)
Any of several broadleaf plants, resistant dextrin and navy bean, above material mixing is stirred, and 16min is handled with tissue mashing machine, worn into entirely with sand mill
The mixed serum that portion passes through 20 mesh；
（2）First step microbe conversion：By weight, brown sugar and black-koji mould filament are weighed, brown sugar is added to step（1）Gained
Mixed serumIn and mix, make solid content be 58%, obtain mixed serum, adjust mixed serumTemperature to 68
DEG C, make mixed serum47 DEG C are cooled to after keeping 90min under conditions of being maintained at 68 DEG C, then is charged with aspergillus niger mycelia
Body simultaneously stirs, and obtains mixed serum, make mixed serumFermentation 70h is carried out under 45 DEG C of temperature conditionss, after
Its filtrate of leaching, obtains zymotic fluid；
（3）Second step microbe conversion：By weight, compound probiotic is weighed, and adds it to step（2）Obtained zymotic fluid
In, stirring mixes both, obtains mixed serum, then by mixed serumSealing, is placed in being fermented under normal temperature, ferments
Unpacking obtains brown sugar prebiotics neokestose comprehensive enzyme after 165 days.
Embodiment 3
A kind of brown sugar prebiotics neokestose comprehensive enzyme provided by the invention and preparation method thereof is as follows：
A kind of brown sugar prebiotics neokestose comprehensive enzyme, is mainly made up of the material of following parts by weight：37 parts of brown sugar, aspergillus niger
0.5 part of mycelia, 3 parts of black grape, 2 parts of Rosa roxburghii, 4.6 parts of black mulberries, 3 parts of black soya bean, 2.5 parts of black glutinous rice, 1.5 parts of Semen sesami nigrum, black wood
1.2 parts of ear, 7 parts of blank corn, 1.2 parts of dateplum persimmon, 8 parts of rye, 1.4 parts of black longan seed, 3.4 parts of black semen litchi, 11 parts of Angelica Keiskei, tangerine
Red 0.9 part of tire fruit, 0.6 part of walnut kernel, 1.6 parts of fructus cannabis, 2 parts of old ginger, 6 parts of pawpaw, 5 parts of dragon fruit, 4 parts of banana, resistance paste
1.8 parts of 13 parts of essence, 2.8 parts of navy bean and compound probiotic.
The present invention also provides the preparation method of brown sugar prebiotics neokestose comprehensive enzyme described above, including following step
Suddenly：
（1）Slurrying：By weight, weigh black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn,
Dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, perfume (or spice)
Any of several broadleaf plants, resistant dextrin and navy bean, above material mixing is stirred, and 18min is handled with tissue mashing machine, worn into entirely with sand mill
The mixed serum that portion passes through 20 mesh；
（2）First step microbe conversion：By weight, brown sugar and black-koji mould filament are weighed, brown sugar is added to step（1）Gained
Mixed serumIn and mix, make solid content be 62%, obtain mixed serum, adjust mixed serumTemperature to 70
DEG C, make mixed serum50 DEG C are cooled to after keeping 70min under conditions of being maintained at 70 DEG C, then is charged with aspergillus niger mycelia
Body simultaneously stirs, and obtains mixed serum, make mixed serumFermentation 72h is carried out under 48 DEG C of temperature conditionss, after
Its filtrate of leaching, obtains zymotic fluid；
（3）Second step microbe conversion：By weight, compound probiotic is weighed, and adds it to step（2）Obtained zymotic fluid
In, stirring mixes both, obtains mixed serum, then by mixed serumSealing, is placed in being fermented under normal temperature, ferments
Unpacking obtains brown sugar prebiotics neokestose comprehensive enzyme after 180 days.
Finished product is made by the formula and preparation method of 3 embodiments of the above respectively, determines the new Coase of brown sugar prebiotics respectively
Containing for various features thing measures its average in sugared comprehensive enzyme solid beverage, as a result as follows：
（1）SOD enzyme activity determinations：By in GB5009.171 health foods superoxide dismutase (SOD) activity measure, as a result
SOD enzyme activities are 2230U/g;
（2）Amylase activity：Determined by GBT/T5521 assay method, as a result amylase activity is 3304U/g;
（3）Lipase activity：By the methods of GB23535 first（Potentiometry）Measure, as a result lipase activity is 2U/g;
（4）Prolease activity：Determined by GB/T23527 prescriptive procedures, as a result prolease activity is 12307U/g;
（5）Prebiotics neokestose content：High performance liquid chromatography（HPLC）Method is measured, chromatographic condition：Using differential refraction
Detector is detected, and chromatographic column is Kromasil-NH2, column temperature is 40 DEG C, and mobile phase is 0.1mol/LCa- ultra-pure waters, flow velocity
For 0.8mL/min, content is calculated using areas of peak normalization method.As a result neokestose content is 30.2%.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (6)
1. a kind of brown sugar prebiotics neokestose comprehensive enzyme, it is characterised in that be mainly made up of the material of following parts by weight：It is black
Sugared 31-37 parts, aspergillus niger mycelia 0.2-0.5 parts, black grape 2-3 parts, Rosa roxburghii 1.5-2 parts, black mulberries 3.8-4.6 parts, black soya bean 2-3
Part, black glutinous rice 2-2.5 parts, Semen sesami nigrum 1-1.5 parts, black fungus 0.8-1.2 parts, blank corn 5-7 parts, dateplum persimmon 0.7-1.2 parts, rye
5-8 parts, black longan seed 1.1-1.4 parts, black semen litchi 2.2-3.4 parts, Angelica Keiskei 6-11 parts, Exocarpium Citri Rubrum tire fruit 0.6-0.9 parts, walnut
Benevolence 0.3-0.6 parts, fructus cannabis 1.2-1.6 parts, old ginger 1-2 parts, pawpaw 4-6 parts, dragon fruit 2-5 parts, banana 2-4 parts, resistance paste
Smart 10-13 parts, navy bean 2-2.8 parts and compound probiotic 1.6-1.8 parts.
2. a kind of brown sugar prebiotics neokestose comprehensive enzyme according to claim 1, it is characterised in that mainly by following
The material of parts by weight is made：It is 35 parts of brown sugar, 0.4 part of aspergillus niger mycelia, 2.5 parts of black grape, 1.8 parts of Rosa roxburghii, 4 parts of black mulberries, black
2.5 parts of beans, 2.3 parts of black glutinous rice, 1.3 parts of Semen sesami nigrum, 1 part of black fungus, 6 parts of blank corn, 1 part of dateplum persimmon, 7 parts of rye, black longan seed
1.2 parts, 2.8 parts of black semen litchi, 8 parts of Angelica Keiskei, 0.7 part of Exocarpium Citri Rubrum tire fruit, 0.5 part of walnut kernel, 1.4 parts of fructus cannabis, old ginger 1.5
1.7 parts of part, 5 parts of pawpaw, 3 parts of dragon fruit, 3 parts of banana, 11 parts of resistant dextrin, 2.4 parts of navy bean and compound probiotic.
A kind of 3. brown sugar prebiotics neokestose comprehensive enzyme according to claim 1 or 2, it is characterised in that：It is described multiple
Probiotics is closed including Lactobacillus rhamnosus, bifidobacterium adolescentis, long discrimination Bifidobacterium, Lactobacillus plantarum, lactobacillus fermenti, guarantor to add
Leah lactobacillus, bifidobacterium breve, bifidobacterium infantis and lactobacillus acidophilus.
A kind of 4. brown sugar prebiotics neokestose comprehensive enzyme according to claim 1 or 2, it is characterised in that：It is described black
Grape, the Rosa roxburghii, the black mulberries, the black soya bean, the black fungus, the blank corn, the Angelica Keiskei, the pawpaw,
The dragon fruit, part banana and the navy bean are fresh wet feed.
A kind of 5. brown sugar benefit according to claim 1 or 2 first neokestose comprehensive enzyme of life, it is characterised in that：Institute
State the brown sugar, black glutinous rice, the Semen sesami nigrum, the dateplum persimmon, the rye, the black longan seed, the black semen litchi, described
Exocarpium Citri Rubrum tire fruit, the walnut kernel, the fructus cannabis, the old ginger, the resistant dextrin and the compound probiotic are sold for market
The siccative sold.
A kind of 6. method for preparing the brown sugar prebiotics neokestose comprehensive enzyme of claim 1 or 2, it is characterised in that bag
Include following steps：
（1）Slurrying：By weight, weigh black grape, Rosa roxburghii, black mulberries, black soya bean, black glutinous rice, Semen sesami nigrum, black fungus, blank corn,
Dateplum persimmon, rye, black longan seed, black semen litchi, Angelica Keiskei, Exocarpium Citri Rubrum tire fruit, walnut kernel, fructus cannabis, old ginger, pawpaw, dragon fruit, perfume (or spice)
Any of several broadleaf plants, resistant dextrin and navy bean, above material mixing is stirred, and 15-18min is handled with tissue mashing machine, ground with sand mill
Into all passing through the mixed serum of 20 mesh；
（2）First step microbe conversion：By weight, brown sugar and black-koji mould filament are weighed, brown sugar is added to step（1）Gained
Mixed serumIn and mix, it is 55-62% to make solid content, obtains mixed serum, adjust mixed serumTemperature extremely
65-70 DEG C, make mixed serum44-50 DEG C is cooled to after keeping 90-100min under conditions of being maintained at 65-70 DEG C, then thereto
Add black-koji mould filament and stir, obtain mixed serum, make mixed serumEnter under 42-48 DEG C of temperature conditionss
Row fermentation 68-72h, after its filtrate of leaching, obtains zymotic fluid；
（3）Second step microbe conversion：By weight, compound probiotic is weighed, and adds it to step（2）Obtained zymotic fluid
In, stirring mixes both, obtains mixed serum, then by mixed serumSealing, is placed in being fermented under normal temperature, ferments
Unpacking obtains brown sugar prebiotics neokestose comprehensive enzyme after 150-180 days.
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