CN107132299A - A kind of multichannel pack tomographic system - Google Patents

Abstract

The invention discloses a multi-channel bunching chromatography system, which consists of a central control system, an extract tank, an elution solvent tank, an elution medium tank, a pressure pump, a multi-channel bunching chromatography column system, a detector and Collection tank composition. Among them, the multi-channel bunching chromatographic column system consists of three parts: a feed storage tank, a bunching chromatographic column composed of ten chromatographic columns, and an elution discharge system. The invention can solve the problems of large occupied area, pressure difference and time difference between separation chromatographic columns, relatively extensive production, lack of intelligence and low monomer target product of separation and purification in the traditional chromatographic separation technology. A multi-channel beam chromatography system device is provided, which can be used for the separation and purification of various biologically active substances such as polyphenols, flavonoids, organic acids, polysaccharides and polypeptides.

Description

A multi-channel beam chromatography system

technical field

The invention relates to a device for separation and purification of an intelligent chromatographic column, which belongs to the field of chemical industry.

Background technique

At present, chromatography and its related application technologies are used in almost all fields, and chromatography technology plays a pivotal role in scientific research and industrial production. It has the characteristics of high separation precision, simple equipment, and convenient operation. According to the different principles of chromatographic separation technology, it can not only be applied to the quantitative analysis and detection of material components, but also can be used for the preparation, separation and purification of biological substances, which is the most important and effective separation for industrial production to obtain high-purity biologically active substances Purification technology. However, the development of chromatographic separation technology on the preparation and industrial scale is still in the initial stage of development. Various chromatographic technologies developed at present have certain shortcomings and deficiencies. The traditional chromatographic separation technology occupies a large area and there is pressure between the separation chromatographic columns. Poor and time difference, relatively extensive production, lack of intelligence. Therefore, the development of continuous operation chromatographic technology with comprehensive characteristics such as intelligence, intensification, precision and wide application has always been expected by people.

This invention solves the problems of large footprint, pressure difference and time difference between separation columns, relatively extensive production, lack of intelligence, and low separation and purification of monomeric target products in traditional chromatographic separation technology. A multi-channel beam chromatography system device is provided, which can be used for the separation and purification of various biologically active substances such as polyphenols, flavonoids, organic acids, polysaccharides and polypeptides.

Contents of the invention

The purpose of the present invention is to solve the problems of large occupied area, pressure difference and time difference between separation chromatographic columns, relatively extensive production, lack of intelligence and low separation and purification of monomeric target products in traditional chromatographic separation technology. A multi-channel beam chromatography system device is provided, which can be used for the separation and purification of various biologically active substances such as polyphenols, flavonoids, organic acids, polysaccharides and polypeptides. The multi-channel bunching chromatography system device of the present invention comprises a central control system, an extract tank, an eluting solvent tank, an eluting medium tank, a pressure pump, a multi-channel bunching chromatography column system, a detector and a collecting tank ; The height h4 and diameter d1 of the collection tanks 1, 2 and 3 are all 1.13m.

The multi-channel bunching column system includes a feed storage tank, a bunching column composed of ten columns and an elution discharge system. The top of the feed storage tank has a water inlet, a feed port and a wide opening window, and the bottom has a medium outlet, and the inside of the feed storage tank communicates with ten chromatographic columns. The height h1 of the feed storage tank is 1.5 m;

The height h2 of the ten chromatographic columns is 3m, the diameter d2 of each chromatographic column is 30cm, and an 80-mesh liner is installed at the bottom of each chromatographic column;

The elution outlet system of the multi-channel bunching chromatographic column system is funnel-shaped, and the height h3 is 15cm. The liquid outlets of the ten chromatographic columns are connected with it. The elution outlet system can be disassembled, and the elution outlet system has backflush valve, waste liquid discharge valve and eluent discharge valve;

The extract tank, the elution solvent tank and the elution medium tank are arranged side by side, and the upper ports of the extract tank, the elution solvent tank and the elution medium tank are respectively connected to the other port of the return pipe through a receiving return valve;

The lower ports of the extract tank, the elution solvent tank and the elution medium tank are respectively connected through a discharge valve and a port of a feed pipe, and a pressure pump is arranged on the feed pipe.

There are n collection tanks in total, n is a natural number, and n=3~10, the upper port of each collection tank is connected to the elution outlet respectively, and the switch of the elution outlet to each collection tank is controlled by the central control system;

The lower port of the collection tank is respectively connected with a solvent recovery system through a discharge valve, and a pressure pump is arranged on the recovery system.

Based on the above-mentioned multi-channel beam-forming chromatographic column system device, it is characterized in that the method involves the following steps:

Step 1, make the ten chromatographic columns in the multi-channel bunching chromatographic column system in parallel mode, transfer the separation and purification medium in the elution medium tank to the feed storage tank through a pressure pump, and then fill it into each chromatographic column column;

Step 2, the material in the extract tank is transferred to the feed storage tank through a pressure pump, and then loaded into each chromatographic column;

Step 3, the elution solvent in the elution solvent tank is transferred to the feed storage tank through a pressure pump, and then each chromatographic column is eluted simultaneously;

Step 4, the detector automatically classifies and collects the eluted biological substances by analyzing and detecting the eluent, and correspondingly enters the collection tanks 1~n;

Step 5, after the elution is completed, open the backflush valve on the elution outlet system and the medium outlet on the feed storage tank to backflush the elution medium in all chromatographic columns.

Advantages of the present invention:

1. It can be used for the separation and purification of various biologically active substances such as polyphenols, flavonoids, organic acids, polysaccharides and polypeptides;

2. It can ensure the pressure consistency of multiple chromatographic columns and the synchronization of feed elution;

3. Integrated design greatly saves space and floor space;

4. The replacement of solid phase medium is convenient and quick;

5. Basically realize the intelligence and automation of chromatographic separation and purification.

Description of drawings

Fig. 1 is a schematic structural view of the multi-channel beamforming chromatography system device of the present invention;

Fig. 2 is the front sectional view of the multi-channel bunching chromatography column of the present invention;

Fig. 3 is a schematic diagram of the central control system of the multi-channel beamforming chromatography system of the present invention.

detailed description

The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

Example 1

As shown in Figure 1, the multi-channel buncher chromatography system provided by the present invention consists of 1 extract storage tank, 2 elution solvent storage tanks, 3 elution medium storage tanks, 4 pressure pumps, 5 central control systems, 8 multi-channel It is composed of bunching column system, 9 detectors, 11 collection tank 3, 12 collection tank 2 and 13 collection tank 1; the height h4 and diameter d1 of collection tanks 1, 2 and 3 are all 1m ~ 4m.

As shown in Figure 1, the multi-channel bunching chromatographic column system consists of three parts: 6 feed storage tanks, 7 dozen chromatographic columns, and 10 elution discharge systems. As shown in Figure 2, the entire multi-channel The channel bunching column system includes 10 chromatographic columns, the medium filled in each chromatographic column can be replaced, 2 refers to the solid area, and the 10 chromatographic columns are integrated to form a cylindrical multi-channel bunching chromatographic column System; the relevant parameters of each chromatographic column: diameter 20 ~ 60 cm, length: 1m ~ 4m, capacity 8L ~ 20L;

As shown in Figure 1, F1, F2 and F3 are respectively the valve switches of 3 elution medium storage tanks, 2 elution solvent storage tanks and 1 extract storage tank; F4 is the medium outlet valve switch; F5 is the recoil valve switch; F6 F7 is the eluent discharge valve switch; F8 is the eluent discharge into the collection tank 1 valve switch; F9 is the eluent discharge into the collection tank 2 valve switch; F10 is the eluent discharge Into the collection tank 3 valve switch;

As shown in Figures 1 and 3, the central control system of the multi-channel bunching chromatographic column system can control valves F1, F2, and F3, thereby operating the entire separation process;

As shown in Figures 1 and 3, the central control system of the multi-channel bunching chromatographic column system can control the valves F8, F9 and F10 after detection and analysis by the detector, so as to realize the classification of different eluting biologically active substances;

The temperature required for the operation of the detector device: 18 ~ 26°C;

The operating pressure of the detector device is 1MPa ~ 8MPa;

The pressure pump 4 of the multi-channel bunching chromatography system is simultaneously used for the substances in the extract tank 1, the elution solvent storage tank 2 and the elution medium storage tank 3 to enter the system, and the relevant parameters of the pressure pump 4: the flow rate is 0.1 mL /min ~ 200 mL/min; working voltage is 220V, 50Hz; power consumption is 1.5 kW ~3.0 kW.

Example 2

As shown in Figure 1, open the valve F1, open the pressure pump 4, and ensure that other valves are closed, so that the ten chromatographic columns in the multi-channel bunching chromatographic column system are in parallel mode, and the separation and purification in the elution medium tank 3 The medium is transferred to the feed storage tank 6 through a pressure pump, and then filled into each chromatographic column by gravity to ensure that the medium is compacted in the chromatographic column;

Open the valve F3, turn on the pressure pump 4, ensure that other valves are closed, transfer the material in the 1 extract tank to the feed storage tank through the pressure pump, and then load the sample into each chromatographic column;

Open valves F2 and F7, turn on pressure pump 4, ensure that other valves are closed, transfer the elution solvent in the elution solvent tank to the feed storage tank through the pressure pump, and then simultaneously elute each chromatographic column; Through the analysis and detection of the eluent, the eluted biological substances are automatically classified and collected, and the valves F8, F9 and F10 are controlled by the PLC central control system, so that the corresponding biologically active substances enter the collection tanks 1~n respectively;

After the elution is completed, open the backflush valve F5 on the elution outlet system and the medium outlet F4 on the feed storage tank to ensure that other valves are closed. By injecting the corresponding washing solvent into the backflush valve F5, all chromatographic The elution medium in the column is backflushed; and the entire chromatographic column system is cleaned.

Claims (7)

1. multichannel pack tomographic system device of the present invention, it is characterised in that the system by central control system, carry Take thing tank, eluting solvent tank, elution media tank, compression pump, multichannel pack chromatogram column system, detector and collecting tank etc. eight Part is constituted.

2. multichannel pack tomographic system according to claim 1, it is characterised in that with multichannel pack chromatographic column system System, pack chromatographic column and elution discharge that the multichannel pack chromatogram column system is made up of feed storage tank, ten root chromatogram columns System is constituted.Feed storage tank is located at the upper end of whole pack chromatographic column, the top of feed storage tank have water inlet, charging aperture and Wide-mouth form, its bottom has inside media outlet, feed storage tank and ten root chromatogram column UNICOMs.The height h1 of feed storage tank is 1.5 m；The height h2 of chromatographic column is 3m；The height h3 for eluting discharge system is 15cm;The height h4 and tank of collecting tank 1,2 and 3 Diameter d1 is 1.13m.

3. central control system according to claim 1, it is characterised in that central control system is by computer, PLC centers Controller and detector composition.The instruction that PLC central controller is obtained according to detector is controlled to valve F1, F2, F3, from And the operation required for being carried out to whole multichannel pack tomographic system.

4. central control system according to claim 1, it is characterised in that central control system is by computer, PLC centers Controller and detector composition.The instruction that PLC central controller is obtained according to detector is controlled to valve F8, F9 and F10, So as to classify to the bioactive substance afforded.

5. multichannel pack tomographic system according to claim 1, it is characterised in that in multichannel pack thin layer chromatography post A variety of solid-phase medias can be filled, various bioactivators are isolated and purified so as to realize.

6. multichannel pack tomographic system according to claim 1, it is characterised in that multichannel pack tomographic system is washed De- tank can be multiple, so as to realize that different solvents are isolated and purified to bioactive substance.

7. multichannel pack tomographic system according to claim 1, it is characterised in that the multichannel pack tomographic system After being isolated and purified to bioactive substance, valve F4 and F5 can be opened, by F5 valves by closing valve F6 and F7 Injection cleaning solvent is cleaned to whole chromatogram column system.