CN106879592A - Glutaraldehyde Deciquam composite disinfectant - Google Patents

Abstract

The invention discloses a glutaraldehyde decyl ammonium bromide composite disinfectant, the main components of which are glutaraldehyde and decyl ammonium bromide, wherein the mass concentration ratio of glutaraldehyde to decyl ammonium bromide is 5:1. The disinfectant with this ratio has a good bactericidal effect, and the synergistic bactericidal effect of the two components can reach the best; the bactericidal effect is strong, the action time is short and the applicability is good; the safety is good, and it has no mutagenic effect on the tk gene of L5178Y cells; The metal has low corrosion and has little impact on port containers, and can be widely used in the disinfection and sterilization of port containers without affecting the reusability of containers.

Description

Glutaraldehyde Decyl Ammonium Bromide Compound Disinfectant

technical field

The invention belongs to the field of disinfectants, in particular to a compound disinfectant of glutaraldehyde decyl ammonium bromide.

Background technique

According to international rules, port sanitation is an important part of entry-exit health and quarantine work, and it is an effective means and measure to prevent the spread and spread of human infectious diseases internationally and to protect human health. Port disinfection is an important part of sanitation treatment at entry-exit ports. Port disinfection includes disinfection and epidemic prevention of port containers, animal and plant products, goods, food, etc. The disinfectant used should meet the following requirements: 1) safe, non-toxic, and low residue. For the disinfection of food contact materials or daily necessities, it is necessary to ensure the safety of the disinfectants used. 2) Quick results. Due to the need for rapid customs clearance at ports, it is impossible to disinfect for a long time, so efficient and rapid anti-virus performance is particularly important. 3) No corrosion. Port containers need to be used repeatedly, and the material is mostly metal, so the disinfectant used should not cause metal corrosion. At the same time, the goods being transported must not be corroded. The daily consumption of port disinfection is very large. Generally, the high-concentration disinfectant is diluted with water in a certain proportion before use. Otherwise, there will be big problems in the daily storage and transportation of the disinfectant. Moreover, the disinfectant is generally an aqueous solution, which is easy to dilute. If Yes other solvents such as alcohol etc. also cause storage and safety issues.

Glutaraldehyde is a commonly used disinfectant component, which can achieve the purpose of disinfection and sterilization by destroying the cell wall, inhibiting the activity of enzymes and the synthesis of DNA and RNA. However, glutaraldehyde has a long action time, is irritating to the mucous membrane, and has a high cost. It is mainly used for the disinfection of medical devices, and it is limited by the cost when used as an environmental disinfectant. Decyl ammonium bromide is a double-chain quaternary ammonium compound, which is also a commonly used disinfectant component. It can change the permeability of bacterial cell membranes, make intracellular components extravasate, hinder cell metabolism, and cause bacterial death. However, decyl bromide has a small antibacterial spectrum, is easily affected by external conditions, and has a limited disinfection range.

There is already a glutaraldehyde decyl ammonium bromide composite disinfectant on the market, the concentration of which is 5g of glutaraldehyde and 5g of decyl ammonium bromide per 100mL disinfectant, which is mainly used for disinfection of farms, public places, and equipment. It needs to be diluted with water in a certain proportion before use. Spraying: routine environmental disinfection, 1:2000-4000 dilution; environmental disinfection 1:500-1000 when epidemics occur. Soaking: disinfection of instruments and equipment, 1:1500～3000. The mass concentration ratio of its two main components, glutaraldehyde and decyl ammonium bromide, is 1:1, but in actual use, it is found that its disinfection effect on various bacteria and fungi is not very good.

Therefore, the present invention improves the existing formula and develops an environmentally friendly formula with better killing effect, more efficient, safe, low residue and non-corrosive for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Type glutaraldehyde decyl ammonium bromide compound disinfectant.

Contents of the invention

The technical problem to be solved by the present invention is to improve a more efficient, safe, low-residue, and non-corrosive environment-friendly glutaraldehyde-decylammonium bromide composite disinfectant suitable for port disinfection.

In order to solve the above-mentioned technical problems, a kind of glutaraldehyde decyl ammonium bromide compound disinfectant provided by the invention, its main component is glutaraldehyde and decyl ammonium bromide, wherein, the mass concentration ratio of glutaraldehyde and decyl ammonium bromide It is 5:1. The disinfectant with this proportion has a good bactericidal effect, and the synergistic bactericidal effect of the two components can reach the best.

Preferably, in the glutaraldehyde decyl ammonium bromide composite disinfectant, glutaraldehyde is 0.05-0.10 mg/mL, decyl ammonium bromide is 0.01-0.02 mg/mL, and the balance is water. The disinfectant with this concentration ratio has a good antibacterial effect on Escherichia coli and Staphylococcus aureus, the killing logarithm value is greater than 5, and the killing rate can reach more than 99.999%.

Preferably, in the glutaraldehyde decyl ammonium bromide composite disinfectant, glutaraldehyde is 0.05 mg/mL, decyl ammonium bromide is 0.01 mg/mL, and the balance is water.

Preferably, in the glutaraldehyde decyl ammonium bromide composite disinfectant, glutaraldehyde is 0.10-0.50 mg/mL, decyl ammonium bromide is 0.02-0.10 mg/mL, and the balance is water. The disinfectant with this concentration ratio has a good antibacterial effect on Pseudomonas aeruginosa and Candida albicans, the killing logarithm value of Pseudomonas aeruginosa is >5, and the killing logarithm value of Candida albicans is >4 .

Preferably, in the glutaraldehyde decyl ammonium bromide composite disinfectant, glutaraldehyde is 0.10 mg/mL, decyl ammonium bromide is 0.02 mg/mL, and the balance is water.

The compound binary disinfectant based on glutaraldehyde decyl ammonium bromide of the present invention has the following advantages:

(1) Strong bactericidal effect. This formula is based on the compound binary disinfectant of glutaraldehyde decyl ammonium bromide, and the suspension quantitative sterilization experiment of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans is carried out, and the results show that the compound disinfectant has a good bactericidal effect , short action time and good applicability.

(2) Good security. This formula is based on the compound binary disinfectant of glutaraldehyde decyl ammonium bromide. The tk gene mutation test of mouse lymphoma cells was carried out to detect the mutagenic effect of the compound disinfectant on the tk gene. The results showed that glutaraldehyde decyl ammonium bromide composite disinfectant had no mutagenic effect on the tk gene of L5178Y cells and was safe.

(3) Metal corrosion is low, and the impact on port containers is small. This formula is based on the composite binary disinfectant of glutaraldehyde decyl ammonium bromide. According to the metal corrosion test results, the composite disinfectant is effective for stainless steel, Common metals such as aluminum and copper are non-corrosive, and can be widely used in the disinfection and sterilization of port containers without affecting the reusability of containers.

detailed description

The technical solution of the present invention will be clearly and completely described below, obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

1. Detection of bactericidal effect of compound disinfectant

Taking Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 8095 and Pseudomonas aeruginosa ATCC 15442 as representatives of the most commonly isolated bacterial propagules in nosocomial infections, and Candida albicans ATCC 10231 as the representative of pathogenic fungi, according to " According to the test method and judgment basis of Disinfection Technical Specifications (2008 Edition), the bactericidal effect test is carried out. The logarithmic value KL of killing bacteria is greater than 5, and the logarithmic value of killing fungi is greater than 4.

1.1. Detect the bactericidal effect of glutaraldehyde decyl ammonium bromide composite disinfectant with different concentration ratios on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans:

Proportion 1: 0.01mg/mL glutaraldehyde and 0.02mg/mL decylmethylammonium bromide (0.5:1);

Proportion 2: 0.02mg/mL glutaraldehyde and 0.02mg/mL decylmethylammonium bromide (1:1);

Proportion 3: 0.06mg/mL glutaraldehyde and 0.02mg/mL decylmethylammonium bromide (3:1);

Proportion 4: 0.10mg/mL glutaraldehyde and 0.02mg/mL decylmethylammonium bromide (5:1);

Proportion 5: 0.14mg/mL glutaraldehyde and 0.02mg/mL decylmethylammonium bromide (7:1);

The action time is 10min, repeats the test and gets the average value for 3 times, the experimental results of each proportioning of glutaraldehyde decyl ammonium bromide composite disinfectant are as shown in table 1:

Table 1: The bactericidal effect of different concentration ratios of glutaraldehyde decyl ammonium bromide composite disinfectant

The above results show that the glutaraldehyde decyl ammonium bromide compound disinfectant with different concentration ratios, when the concentration ratio of glutaraldehyde and decyl ammonium bromide gradually increases, is effective against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and the bactericidal effect of Candida albicans; when the concentration ratio of glutaraldehyde and decyl ammonium bromide was 5:1, the best bactericidal effect could be achieved compared with other groups of concentration ratios, and the synergistic bactericidal effect of the two components could Reached the best; when the concentration ratio of glutaraldehyde and decyl ammonium bromide continued to increase to 7:1, the bactericidal effect was not significantly improved compared with that of 5:1. And considering the higher cost of glutaraldehyde in the two components, it is more preferred that 5:1 is the optimal ratio.

1.2. Detect the bactericidal effect of different concentrations of glutaraldehyde decyl ammonium bromide composite disinfectant (5:1) on Escherichia coli and Staphylococcus aureus:

Proportion 6: 0.025mg/mL glutaraldehyde and 0.005mg/mL decyl ammonium bromide;

Proportion 7: 0.05mg/mL glutaraldehyde and 0.01mg/mL decyl bromide;

Proportion 8: 0.075mg/mL glutaraldehyde and 0.015mg/mL decylmethylammonium bromide;

Proportion 9: 0.10mg/mL glutaraldehyde and 0.02mg/mL decyl bromide;

The action time is 10min, repeats the test and gets the average value for 3 times, the experimental results of each proportioning of glutaraldehyde decyl ammonium bromide composite disinfectant are as shown in table 2:

Table 2: Bactericidal effect of different concentrations of glutaraldehyde decyl ammonium bromide composite disinfectant (5:1)

The above results illustrate that under the condition that the concentration ratio is 5:1, when the concentration of glutaraldehyde and decyl ammonium bromide reaches 0.05mg/mL and 0.01mg/mL respectively, its killing of Escherichia coli and Staphylococcus aureus The logarithm values are all greater than 5, and the killing rate can reach more than 99.999%, meeting the requirements. When the concentration continues to increase, the bactericidal effect can still be improved. However, considering the cost of use and the high concentration of disinfectant that may cause residual problems, the bactericidal concentration for Escherichia coli and Staphylococcus aureus can be controlled as glutaraldehyde 0.05-0.10 mg/mL and decyl ammonium bromide 0.01-0.02 mg/mL, preferably glutaraldehyde 0.05 mg/mL and decyl ammonium bromide 0.01 mg/mL.

1.3. Detect the bactericidal effect of different concentrations of glutaraldehyde decyl ammonium bromide composite disinfectant (5:1) on Pseudomonas aeruginosa and Candida albicans:

Proportion 10: 0.05mg/mL glutaraldehyde and 0.01mg/mL decylmethyl ammonium bromide;

Proportion 11: 0.10mg/mL glutaraldehyde and 0.02mg/mL decylmethyl ammonium bromide;

Proportion 12: 0.20mg/mL glutaraldehyde and 0.04mg/mL decyl bromide;

Proportion 13: 0.50mg/mL glutaraldehyde and 0.10mg/mL decyl bromide;

The time of action is 10min, repeats the test and gets the average value for 3 times, the experimental results of the different concentrations of glutaraldehyde decyl ammonium bromide composite disinfectant are as shown in table 3:

Table 3: The bactericidal effect of different concentrations of compound disinfectant (5:1) on Pseudomonas aeruginosa and Candida albicans

The above results illustrate that under the condition that the concentration ratio is 5:1, when glutaraldehyde and decyl ammonium bromide are respectively 0.10mg/mL and 0.02mg/mL, the logarithmic value of killing Pseudomonas aeruginosa > 5, The logarithmic value of killing Candida albicans is more than 4, which meets the requirements, and the disinfectant has a good antibacterial effect. When the concentration continues to increase, the bactericidal effect can still be improved.

1.4. detect the sterilization time of glutaraldehyde decyl ammonium bromide composite disinfectant of the present invention:

Use the compound disinfectant of 0.10mg/mL glutaraldehyde and 0.02mg/mL decyl ammonium bromide to screen the sterilization time according to the action time of 2.5min, 5min, 10min, and 20min, repeat the test 3 times and take the average value, the results are shown in the table 4. Based on the "Technical Specifications for Disinfection" (2008 edition).

Table 4: Bactericidal time of compound disinfectants against Pseudomonas aeruginosa and Candida albicans

The above results show that the compound disinfectant of 0.10mg/mL glutaraldehyde and 0.02mg/mL decyl ammonium bromide can kill Pseudomonas aeruginosa in the shortest time of 5min, and the shortest time of killing Candida albicans is 10min, and the effect is quick , suitable for port disinfection.

Using the same method, kill Escherichia coli and Staphylococcus aureus with a compound disinfectant of 0.05mg/mL glutaraldehyde and 0.01mg/mL decylmethylammonium bromide. Reaching above 5, the killing effect is good and the effect is quick.

2. Toxicity testing of compound disinfectants

Taking L5178Y cells as the experimental object, the tk gene mutation test of mouse lymphoma cells was used to detect the gene mutation effect of compound disinfectants on mammalian cells cultured in vitro. Based on GB 15193.20-2014 "In Vitro Mammalian Cell TK Gene Mutation Test" as the basis for judging tk gene mutation, the test was carried out under two conditions of activation and non-activation, and the positive control in the S9 metabolic activation system was cyclophosphamide (3 μg/mL ); the positive control in the non-activated system is methyl methanesulfonate (10 μg/mL), and the test results are shown in Table 5.

Table 5: Mutagenicity of compound disinfectants on tk gene of L5178Y cells

Above-mentioned result shows, under two kinds of conditions, compound disinfectant group all does not reach more than 3 times of vehicle control, does not have more than one dose significantly higher than vehicle control, does not have dose-response trend, so it is judged as negative. That is to say, glutaraldehyde decyl ammonium bromide composite disinfectant has no mutagenic effect on the tk gene of L5178Y cells under the two conditions of activation and non-activation.

3. Metal corrosion detection of compound disinfectant

The preparation concentration is 0.5mg/mL glutaraldehyde+0.1mg/mL decyl bromide (5 times of the minimum action concentration of Pseudomonas aeruginosa 0.10mg/mL glutaraldehyde and 0.02mg/mL decyl bromide; ) for metal corrosion tests. Select stainless steel, carbon steel, aluminum, and copper four metal discs, hang them in the disinfectant solution, and soak them for 72 hours at a time. After soaking for a specified time, take out the metal sheet, compare the weight after treatment with that before soaking, calculate the metal corrosion rate (R), and judge its metal corrosion according to the "Disinfection Technical Specifications" (2008 edition). The results are shown in Table 6.

Table 6: Metal Corrosivity of Compound Disinfectant at 5 Times Concentration

Above-mentioned result shows, the compound disinfectant (5 times concentration) of 0.5mg/mL glutaraldehyde+0.1mg/mL decyl methyl ammonium bromide is substantially non-corrosive to stainless steel, aluminum, copper, has slight corrosion to carbon steel; But Compared with water, its corrosiveness is equivalent, and there is no significant difference. From this it can be seen that

In summary, the above-mentioned embodiments are only preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements, Improvements and the like should all be included within the protection scope of the present invention.

Claims (5)

1. a kind of glutaraldehyde Deciquam composite disinfectant, its main component is glutaraldehyde and Deciquam, it is characterised in that penta Dialdehyde is 5 with the mass concentration ratio of Deciquam:1.

2. in glutaraldehyde Deciquam composite disinfectant as claimed in claim 1, it is characterised in that glutaraldehyde be 0.05~ 0.10mg/mL, Deciquam is 0.01~0.02mg/mL, balance of water.

3. in glutaraldehyde Deciquam composite disinfectant as claimed in claim 2, it is characterised in that glutaraldehyde is 0.05mg/mL, Deciquam is 0.01mg/mL, balance of water.

4. in glutaraldehyde Deciquam composite disinfectant as claimed in claim 1, it is characterised in that glutaraldehyde be 0.10~ 0.50mg/mL, Deciquam is 0.02~0.10mg/mL, balance of water.

5. in glutaraldehyde Deciquam composite disinfectant as claimed in claim 4, it is characterised in that glutaraldehyde is 0.10mg/mL, Deciquam is 0.02mg/mL, balance of water.