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CN106726557B - Blood collection bag and method for removing fibrinogen by using same - Google Patents

Blood collection bag and method for removing fibrinogen by using same Download PDF

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Publication number
CN106726557B
CN106726557B CN201710046727.7A CN201710046727A CN106726557B CN 106726557 B CN106726557 B CN 106726557B CN 201710046727 A CN201710046727 A CN 201710046727A CN 106726557 B CN106726557 B CN 106726557B
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blood
bag body
bag
pipe
blood collection
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CN106726557A (en
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周建国
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Hangzhou Poly Medical Products Co Ltd
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Hangzhou Poly Medical Products Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61JCONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A61J1/10Bag-type containers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/15Devices for taking samples of blood
    • A61B5/150007Details
    • A61B5/150366Blood collection bags, e.g. connected to the patient by a catheter comprising means for removing a small sample of collected blood from the bag
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61JCONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14Details; Accessories therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)

Abstract

The invention discloses a blood collection bag which comprises a bag body, wherein a porous material is arranged in the bag body, and the porous material is porous cotton. The invention has the following advantages and effects: the porous cotton serving as the porous material is arranged in the bag body, after the bag body is placed on a shaking table to shake, when fibrinogen in blood in the bag body is converted into insoluble fibrin, the generated insoluble fibrin can be adsorbed after contacting the porous cotton, the fibrinogen in the blood is removed in the closed bag body, and the effects of avoiding blood coagulation in the bag body, simplifying the preparation process of defibered blood and reducing the pollution hidden trouble in the preparation process are achieved.

Description

Blood collection bag and method for removing fibrinogen by using same
Technical Field
The invention relates to the technical field of blood defibration, in particular to a blood collection bag.
Background
Blood is a viscous liquid, consisting of plasma and blood cells. Defibrinated blood is a mixture obtained by removing fibrinogen from blood, and its components are mostly known, but some of them are not clear. Defibrinated animal blood, such as defibrinated sheep whole blood, is often used to provide nutrients, hormones, growth factors, and the like in bacterial culture. The blood agar culture medium containing defibrinated sheep whole blood is a main means for detecting whether a patient is infected by bacteria in a hospital clinical laboratory, and a doctor judges whether the patient is pathogenic by the bacterial infection according to a detection result and performs a drug sensitive experiment on detected infectious bacteria to determine an effective antibiotic for treatment, so that the potential hazard of antibiotic abuse can be effectively prevented. In the preparation process of the defibered blood, the animals need to be subjected to blood collection through a blood collection bag, and the defibered blood is obtained after fibrinogen in the blood is removed. There are many methods for separating fibrinogen from blood, and the fibrinogen in blood is generally removed by a glass bead method.
The glass bead method is to inject blood into a blood collection bottle with glass beads, and rotate the blood collection bottle continuously to wind fibrin on the glass beads, so that fibrinogen can be removed and blood coagulation can be prevented. Blood needs to flow into a blood collection bottle, after fibrin is separated by continuous rolling of glass beads, the blood needs to pass through a special filtering device, the fibrin and the glass beads are filtered, and then the obtained blood flows into a closed storage system for storage, the operation process is long, the manufacturing procedures are multiple and complicated, a large number of blood cells are damaged due to the mechanical action generated by the collision and rolling of the glass beads (the blood cells are one of important effective components for judging whether bacteria are infected or not and judging the type of the infected bacteria in hospital inspection), containers and pipelines which are contacted in the defibered blood preparation process are more, and a plurality of procedures are open operation in the preparation process. In fact, the defibrinated sheep whole blood supplied in the market at present has high pollution rate, and the safety and the effectiveness of the blood agar culture medium are seriously influenced, and the reliability of the bacteria test result is also influenced.
Disclosure of Invention
The invention aims to provide a blood collection bag which has the effects of continuously completing the processes of separating and filtering fibrin in a sealing system at one time, simplifying the preparation process of defibered blood, basically eliminating the influence of the environment on the safety of the blood in the collection process, completely eradicating the damage of the effective components of the blood due to the mechanical action and reducing the uncertain influence of vessels on the blood due to the fact that the used materials are disposable consumables.
The technical purpose of the invention is realized by the following technical scheme: a blood collection bag comprises a bag body, wherein a porous material is arranged in the bag body.
By adopting the technical scheme, the porous material is a material with a certain size and number of pore structures, and the porous material usually has adsorption capacity, and because atoms on the surface of the porous material are acted by asymmetric force, a residual force field is formed, and the expression of an attraction effect on external substances is generated, wherein the expression comprises two chemical bond forces of physical adsorption force Van der Waals force and chemical adsorption force. After the porous material is placed in the bag body, after soluble fibrinogen in blood is converted into insoluble fibrin, the insoluble fibrin contacts the porous material and enters or partially enters the porous material to be adsorbed by the porous material, so that blood coagulation in the blood collection bag is avoided, fibrinogen can be directly removed in the closed blood collection bag body, defibered blood is obtained, the preparation flow of the defibered blood is shortened, and the hidden danger of pollution in the preparation process is reduced.
The invention is further provided with: the porous material is porous cotton.
By adopting the technical scheme, the porous material is porous cotton which is a porous material and has good water absorption and elasticity. The porous cotton is placed in the bag body, and can adsorb insoluble fibrin in blood in the bag body.
The invention is further provided with: the porous cotton is fixed in the middle of the bag body.
Through adopting above-mentioned technical scheme, porous cotton is fixed at bag body middle part, and the internal blood of bag can contact porous cotton when rocking the bag body.
The invention is further provided with: the blood collection bag is characterized in that the bag body is provided with a liquid inlet pipe communicated with the inside of the bag body, a blood collection pipe communicated with the middle part of the liquid inlet pipe, and a blood collection needle arranged on the blood collection pipe.
Through adopting above-mentioned technical scheme, puncture into the animal blood vessel with the blood taking needle in, blood in the animal blood vessel flows into the heparin tube from the blood taking needle, flows into the feed liquor pipe from the heparin tube again, and the rethread feed liquor pipe enters into the internal of bag.
The invention is further provided with: the blood sampling tube is connected with the liquid inlet tube at a joint, and a pair of tube clamps are arranged on the liquid inlet tube and distributed on two sides of the joint.
Through adopting above-mentioned technical scheme, when needs acquire animal blood sample, open the pipe clamp that lies in connected node and keep away from bag body one side on the feed liquor pipe, the one end that the bag body was kept away from to the blood of feed liquor pipe from the feed liquor pipe flows out, is convenient for acquire the blood sample.
The invention is further provided with: the bag body is provided with a sample bag, the sample bag is communicated with the inside of the bag body through a connecting pipe, and the connecting pipe is provided with a pipe clamp.
By adopting the technical scheme, the bag body is provided with the connecting pipe and the pipe clamp, whether fibrinogen in blood generated in the bag body needs to be removed or not is detected, the pipe clamp on the connecting pipe is opened, the blood in the bag body flows into the sample bag, then the pipe clamp is used for connecting the pipe clamp, the connecting pipe is sealed, and the sample bag is taken down from the bag body, so that a sample in the bag body can be obtained.
Another object of the present invention is to provide a method for removing fibrinogen using the above blood collection bag.
The technical purpose of the invention is realized by the following technical scheme: comprises the following steps of A, collecting blood, namely collecting animal blood by using a blood collecting needle; and step B, continuously shaking the bag body to enable fibrinogen in blood in the bag body to be adsorbed on the porous material.
Through adopting above-mentioned technical scheme, at first penetrate the animal blood vessel with the blood taking needle, the blood in the animal blood vessel flows into the internal of bag from the blood taking needle, and the bag body that shakes constantly again makes the internal blood of bag fully contact porous material, and the insoluble fibrin that produces in the blood adsorbs on porous material, gets rid of fibrinogen from the blood, obtains the defibrination blood.
The invention is further provided with: and B, fixing the bag body on a shaking table, starting the shaking table, and continuously shaking the bag body.
By adopting the technical scheme, the shaking table is called as an oscillator, is a common laboratory device, belongs to a biochemical instrument, and is widely used for shaking up liquid and culturing micro-animals and cells. The bag body is fixed on the shaking table, the shaking is uniform, and the manual operation is reduced.
In conclusion, the invention has the following beneficial effects: the porous cotton serving as the porous material is arranged in the bag body, after the bag body is placed on a shaking table to shake, when fibrinogen in blood in the bag body is converted into insoluble fibrin, the generated insoluble fibrin can be adsorbed after contacting the porous cotton, the fibrinogen in the blood is removed in the closed bag body, and the effects of avoiding blood coagulation in the bag body, shortening the preparation flow of defibered blood and reducing the pollution hidden trouble in the preparation process are achieved.
Drawings
FIG. 1 is a schematic structural view of example 1;
fig. 2 is a schematic view of the pipe clamp structure of embodiment 1.
In the figure: 1. a bag body; 11. a liquid inlet pipe; 111. connecting the nodes; 12. a liquid outlet pipe; 2. a porous material; 3. a blood collection tube; 31. a blood collection needle; 4. a sample bag; 5. a connecting pipe; 51. a first pipe body; 52. a second tube body; 6. a pipe clamp; 61. a clip body; 611. a limiting surface; 62. locking the hook; 621. hooking a groove; 622. a guide surface; 63. an elastic section; 631. an arc-shaped surface; 64. a clamping block; 65. and a through hole.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The specific embodiments are only for explaining the present invention, and the present invention is not limited thereto, and those skilled in the art can make modifications without inventive contribution to the present embodiments as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Example 1: the utility model provides a blood collection bag, as shown in figure 1, includes bag body 1, communicates feed liquor pipe 11 and drain pipe 12 in bag body 1, and bag body 1 middle part is provided with porous material 2, and porous material 2 is porous cotton, and the porous cotton is put into bag body 1 back and is extruded and take place elastic deformation, and both ends terminal surface supports tightly on the inner wall of bag body 1. Feed liquor pipe 11 middle part intercommunication has heparin tube 3, and feed liquor pipe 11 is connected for connected node 111 with heparin tube 3 junction, is provided with two pipe clamps 6 on the feed liquor pipe 11, and 6 distributions of pipe clamp are in connected node 111 both sides. The liquid outlet pipe 12 is also provided with a pipe clamp 6. The bag body 1 is also connected with a sample bag 4, and the sample bag 4 is communicated with the bag body 1 through a connecting pipe 5. The connecting tube 5 comprises a first tube 51 communicated with the bag body 1 and a second tube 52 communicated with the sample bag 4, the first tube 51 is inserted into the second tube 52, and the outer wall of the first tube 51 abuts against the inner wall of the second tube 52. The first pipe body 51 is provided with a pipe clamp 6.
As shown in fig. 2, the pipe clamp 6 includes a clamp body 61, a locking hook 62 integrally disposed at one end of the clamp body 61, and an elastic section 63 integrally disposed at the other end of the clamp body 61, wherein the locking hook 62 and the elastic section 63 are both provided with a through hole 65 for the liquid inlet pipe 11 or the liquid outlet pipe 12 to pass through. The lock hook 62 is provided with an arc-shaped guide surface 622 and a groove, one end of the elastic section 63, which is far away from the clamp body 61, is provided with an arc-shaped surface 631, the elastic section 63 is integrally provided with a clamping block 64, and the clamp body 61 is provided with a limiting surface 611 corresponding to the clamping block 64.
When the pipe clamp 6 is placed on the liquid inlet pipe 11, the liquid inlet pipe 11 sequentially passes through the through hole 65 on the lock hook 62, the gap between the fixture block 64 and the limiting surface 611, and the through hole 65 on the elastic section 63, so that the elastic section 63 is bent towards the lock hook 62, the arc-shaped surface 631 on the elastic section 63 contacts the guide surface 622 on the lock hook 62, the guide surface 622 on the lock hook 62 is stressed to drive the lock hook 62 to be away from the elastic section 63, when the elastic section 63 is located at the hook 621 of the lock hook 62 after leaving the guide surface 622, the guide surface 622 on the lock hook 62 is no longer under the acting force of the elastic section 63, the lock hook 62 moves towards the elastic section 63, and the end of the elastic section 63 is embedded into the hook 621. Meanwhile, the fixture block 64 moves towards the limiting surface 611, and the liquid inlet pipe 11 is tightly abutted against the limiting surface 611, so that the pipeline of the liquid inlet pipe 11 is sealed. When the locking hook 62 is opened in a direction away from the elastic section 63, the elastic section 63 recovers elastic deformation after the end of the elastic section 63 leaves the groove, and the fixture block 64 is away from the limiting surface 611, so as to loosen the pipeline of the liquid inlet pipe 11.
Example 2: a method for removing fibrinogen by using the blood collection bag comprises the step A of collecting blood, wherein the blood collection needle 31 penetrates into an animal blood vessel to collect blood, the blood enters the bag body 1 after passing through the blood collection needle 31, the blood collection tube 3 and the liquid inlet tube 11 in sequence, the tube clamp 6 on the liquid inlet tube 11 is closed, the liquid inlet tube 11 is sealed, and then the blood collection needle 31 is taken out of the animal blood vessel. And step B, fixing the bag body 1 on a shaking table, selecting an animal constant-temperature culture shaking table as the shaking table, starting the shaking table to shake for 10 hours, and taking down the bag body 1 to obtain the defibered blood.
Fibrinogen detection assay: the sheep is collected with the blood collection bag, the tube clamp 6 on the connecting tube 5 is opened to make the blood in the bag body 1 flow into the sample bag 4, and the tube clamp 6 on the first tube body 51 is used for clamping the first tube body 51 to seal the first tube body 51. The second tube 52 is forcibly pulled out from the first tube 51, fibrinogen in the sample bag 4 is detected by a hemagglutination meter, and the arithmetic mean value is taken after three detections, and the detection result is that the fibrinogen content in the sample is 0.12 g/L. Indicating that the porous material 2 can effectively remove fibrinogen in blood.

Claims (5)

1. A blood collection bag, includes bag body (1), its characterized in that:
a porous material (2) is arranged in the bag body (1);
the blood collection bag is characterized in that a liquid inlet pipe (11) communicated in the bag body (1), a blood collection pipe (3) communicated with the middle part of the liquid inlet pipe (11) and a blood collection needle (31) installed on the blood collection pipe (3) are arranged on the bag body (1), the blood collection pipe (3) is connected to the liquid inlet pipe (11) to form a connection node (111), and a pair of pipe clamps (6) distributed on two sides of the connection node (111) are arranged on the liquid inlet pipe (11);
the bag body (1) is provided with a sample bag (4), the sample bag (4) is communicated with the bag body (1) through a connecting pipe (5), the connecting pipe (5) comprises a first pipe body (51) communicated with the bag body (1) and a second pipe body (52) communicated with the sample bag (4), the first pipe body (51) is inserted into the second pipe body (52), and the first pipe body (51) is provided with a pipe clamp (6).
2. A blood collection bag according to claim 1, wherein: the porous material (2) is porous cotton.
3. A blood collection bag according to claim 2, wherein: the porous cotton is fixed in the middle of the bag body (1).
4. A method of removing fibrinogen using the blood collection bag of any one of claims 1 or 2, comprising: comprises the steps of A, collecting blood, namely collecting animal blood by using a blood collecting needle (31); and step B, continuously shaking the bag body (1) to enable fibrin in blood in the bag body (1) to be adsorbed on the porous material (2).
5. The method of claim 4, wherein the fibrinogen is removed from the blood collection bag by: and in the step B, the bag body (1) is fixed on a shaking table, and the shaking table is started to continuously shake the bag body (1).
CN201710046727.7A 2017-01-22 2017-01-22 Blood collection bag and method for removing fibrinogen by using same Active CN106726557B (en)

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CN107202899A (en) * 2017-07-10 2017-09-26 四川省医学科学院·四川省人民医院实验动物研究所 A kind of authentication method of good chief of a tribe monkey blood group
CN110063731A (en) * 2019-04-02 2019-07-30 青岛中科爱博生物科技有限公司 The sterile de- fiber animal's whole blood acquisition device of one kind and method
CN113069613A (en) * 2021-03-22 2021-07-06 广州市天河诺亚生物工程有限公司 Navel cord blood rewarming leak protection box

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CN2249612Y (en) * 1995-11-28 1997-03-19 中国人民解放军第一五七医院 Blood collecting bag with filter
JP2000309539A (en) * 1999-04-28 2000-11-07 Toyobo Co Ltd Obtaining blood serum from blood plasma
CN100343667C (en) * 2003-01-22 2007-10-17 积水化学工业株式会社 Filter for removing fibrinogen, filter device for removing fibrinogen, and method of removing fibrinogen using the same
EP1621220B1 (en) * 2003-05-08 2014-11-19 Kaneka Corporation Low density lipoprotein/fibrinogen adsorbent and adsorption apparatus capable of whole blood treatment
US8796017B2 (en) * 2003-05-21 2014-08-05 Jms Co., Ltd. Container for preparing serum and regenerative medical process using the same
EP2679302A1 (en) * 2012-06-28 2014-01-01 Zentrum für biomedizinische Technologie der Donau- Universität Krems Selective sorption agent for extracorporeal blood purification
CN204411291U (en) * 2014-12-24 2015-06-24 内蒙古维克生生物科技有限公司 A kind of production equipment of former blood of defibrinating for new born bovine blood

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